Search Results (958)

Miang, a traditional fermented tea produced from Camellia sinensis var. assamica, is of notable cultural and socio-economic relevance in Northern Thailand. Traditionally, the non-filamentous fungi-based process (NFP) in western Lanna uses only young tea leaves, resulting in substantial amounts of mature leaves being discarded as agricultural waste. This study aimed to utilize the mature tea leaves by adapting the filamentous fungi growth-based process (FFP) of eastern Lanna using selected tannin-tolerant microorganisms, including Aspergillus niger MLF3, Cyberlindera rhodanensis P3, and Lactiplantibacillus pentosus A14-6. Study on fermentation dynamics and bioactive compound formation based on a 2-step fermentation process: 3-day solid-state fermentation with A. niger MLF3, followed by 7-day submerged fermentation by co-culture of C. rhodaninsis P3, and L. pentosus A14-6 in 500 mL sterile distilled water at 30 °C. Increased activities of polysaccharide-degrading enzymes and organic acids were clearly observed during solid-state fermentation, while the significant changes in polyphenol, antioxidant, and reducing sugar content in cell-free supernatant (CFS) were found after submerged fermentation. The obtained CFS shows inhibitory effects of 90 ± 2.5% and 95 ± 1.8% on α-glucosidase and α-amylase, respectively. Analysis of CFS by E-tongue and E-nose clearly indicated the influence of microbial mixture on the taste and aroma of the fermented products. These results demonstrate not only a high-yielding strategy for the effective biotransformation of mature tea leaves into functional drink products but also significant implications for reducing agricultural waste. Full article

Small- and mid-sized municipalities increasingly need to assess the long-term effectiveness of urban greening projects under climate change, but typically lack the resources for extensive measurement campaigns or complex simulation infrastructure. We propose and demonstrate a practical three-component assessment framework that can be deployed with limited resources: one or two UAV thermal surveys to capture the spatial baseline, a small network of fixed monitoring stations for temporal context, and a single microscale model to project future scenarios. We apply this framework to a 0.5 ha parking-to-park conversion in Aalen, Germany (pop. 68,000). A diurnal drone survey (four flights over 14 h) established surface temperature contrasts of up to 34 K between sealed and tree-shaded areas. Six fixed stations operating over six weeks confirmed that an existing mature linden reduced hot days by nearly 50%. The ASMUS_green microscale model, evaluated against the drone observations (mean absolute difference 3.4 K across surface types), was then used to project Physiologically Equivalent Temperature (PET) under EURO-CORDEX scenarios for 2035 (+1.3 K) and 2055 (+2.5 K) combined with tree growth. The results show that young trees provide limited near-term relief, but mature canopy reduces PET by 6–8 K, offsetting the projected warming. We discuss how each component compensates for the limitations of the others, making the combined framework more robust than any single method. The approach is designed to be transferable to other municipalities facing similar planning decisions with comparable resource constraints. Full article

Background: Anterior cruciate ligament (ACL) injuries are increasing in young athletes and many are related to non-contact, spontaneous mechanical fatigue-related ruptures. The objective of this narrative review is to identify and synthesize the anatomical, histological, physiological, and biomechanical basis of extracellular matrix (ECM) factors that contribute to ACL injuries and suggest ways to decrease their occurrence. Methods: The primary investigator searched PubMed, Web of Science, and Google Scholar database titles and abstracts using search phrases with Boolean operators: “anterior cruciate ligament” OR “ACL”, OR “cranial cruciate ligament” AND “disease”; “anterior cruciate ligament” OR “ACL”, OR “cranial cruciate ligament” AND “spontaneous rupture” OR “non-contact injury”; and “anterior cruciate ligament” OR ACL, OR cranial cruciate ligament” AND “crosslink”, “collagen” OR “extracellular matrix”; and “anterior cruciate ligament” OR “ACL”, OR “cranial cruciate ligament” AND “microtrauma”, OR “sudden” OR “fatigue failure”. The primary investigator and a sports orthopedic surgeon reviewed titles and abstracts of diverse evidence sources. From these identified sources, the study team performed full text reviews, selected contributing articles, performed Strength of Recommendation Taxonomy (SORT) grading, and synthesized the following themes: A Hostile Environment, ACL Strain, and Poor Nutrient Delivery; Accumulative ACL Microtrauma and Mechanical Failure; The ACL Differs From Other Ligaments; Collagen, the ECM, and ACL Mechanobiology; Crimps and ACL ECM Stretch; Crosslinks Improve ECM Mechanical Properties; The Delicate Collagen Synthesis and Degradation Balance; Exercise Training and the ACL; Can Nutraceuticals Help Restore the Balance?; Training Induced ACL Hypoxia; Estrogen and the Female Athlete; Counting Pitches or Counting Collagen Fiber Ruptures; and Restoring A Positive Anabolic–Catabolic Collagen Balance. Results: Regular exercise training within a physiologically safe loading range is vital to ACL ECM health. However, low or moderate evidence suggested that poor blood supply, slow metabolism, and a hypoxic environment may unbalance anabolic and catabolic homeostasis. Active rest and recovery concepts that prevent youth baseball shoulder and elbow injuries may help prevent non-contact ACL injuries. Conclusions: More prescriptive active rest and recovery intervals and neuromuscular control training may restore the anabolic–catabolic balance that increases mature crosslink density and improves ACL ECM strength. Confirmatory studies are needed to better establish therapeutic intervention mode(s), timing, dosage, and frequency optimization. Full article

Ventricular repolarization abnormalities are among the most frequent electrocardiographic findings in pediatric athletes undergoing cardiovascular screening, yet their clinical significance remains a major source of diagnostic uncertainty. While most of them represent benign expressions of training-induced cardiac remodeling and developmental maturation, selected patterns may constitute the earliest phenotypic manifestation of cardiomyopathies or primary electrical disease. Distinguishing physiological adaptation from early pathology is therefore essential to prevent both sudden cardiac events and unnecessary restrictions on sports participation. This review integrates contemporary international electrocardiographic interpretation criteria with emerging pediatric evidence to provide a clinically oriented framework for evaluation and risk stratification of ventricular repolarization abnormalities in pediatric athletes. Early repolarization and anterior T-wave inversion are commonly benign when occurring within recognized age- and ethnicity-specific patterns and in the absence of symptoms, concerning family history, or structural abnormalities. Conversely, lateral or inferolateral T-wave inversion, atypical ST-segment morphology, complex ventricular arrhythmias, and abnormal imaging findings represent red flags requiring comprehensive investigation, including multimodality imaging when indicated. Due to the dynamic electrophysiological evolution during adolescence, longitudinal reassessment is crucial. A structured, risk-based approach integrating electrocardiographic features, demographic/familial context, clinical evaluation, imaging findings, and follow-up provides a pragmatic strategy to optimize risk detection while safeguarding appropriate athletic participation in young athletes. Full article

In elite youth soccer, the objective is to identify, develop, and enhance players’ ability to support their progression. During adolescence, players of the same chronological age often show differences in technical, tactical, physical, and psychological performance due to variations in biological maturation. The bio-banding (BB) format tries to reduce these discrepancies by grouping players with maturity-matched peers, promoting development within a maturity-respecting environment. This review synthesizes the effects of BB on soccer-specific performance in comparison to traditional chronological-age (CA) grouping. PubMed, Scopus, Web of Science (Core and Medline), and BASE databases were searched, and experimental studies using crossover, such as those applying both BB and CA in young soccer players, were considered eligible. Eleven experimental studies were included. Most of the investigated outcomes focused on physical performance (n = 9) and technical and tactical characteristics (n = 8), while psychological aspects were less examined (n = 2). Moreover, two studies further assessed how different BB methods influenced the investigated outcomes. The evidence confirms that BB influences youth soccer player characteristics, showing differences compared to CA grouping. BB can be an approach for optimizing individual growth but is not a definitive solution, presenting limits that require careful management, appropriate challenge, and integration with injury prevention and workload monitoring. Further research is needed to clarify its performance-related impact across maturity statuses. Full article

Sympatric butterflies in the same family (Pieridae) utilize the same genus of hostplant (Fabaceae), yet some are only found as caterpillars on young foliage, while others appear to eat mature foliage. Observations conducted over the course of a single year on five species of Inga (Fabaceae) in Monteverde, Costa Rica, revealed three butterflies whose caterpillars consumed their leaves. One species was more common throughout the year with caterpillars encountered on primarily mature foliage; two others were found only on species with new leaves and occurred seasonally, during months of increased new leaf production. In feeding preference tests, those species showed a marked preference for eating new leaves, whereas the more common species showed no preference. The seasonality of occurrence of the two species eating new leaves may be explained, in part, by availability of their preferred foliage. The coexistence of the three butterflies using the same host-plants may be facilitated by their leaf age preferences and differential oviposition patterns of the females. Full article

Breeding in bird species inhabiting the temperate climate zone is strongly seasonally regulated. One such species is the common pheasant (Phasianus colchicus), widely maintained in aviary systems and of considerable importance in game management. The aim of this study was to determine seasonal variability in selected morphological traits of male common pheasants and to assess their relationship with vocalisation parameters during the annual cycle. The study was conducted on males kept under aviary conditions. Traits related to reproductive condition were analysed, including testicular mass and facial wattle height, together with mating vocalisations. The results revealed clear seasonal variation in the analysed traits. A gradual increase in body mass and spur length was observed from summer to winter, reflecting the maturation of young males and accumulation of energy reserves before the breeding season. The highest values of reproductive parameters, particularly testicular mass and wattle height, were recorded in spring, indicating peak hormonal activity and full sexual maturity. In June, a marked regression of these traits and a decline in the somatic condition traits were observed, corresponding to the end of the breeding season. Changes in vocal activity followed a similar pattern, suggesting a link between acoustic parameters and seasonal reproductive dynamics. Full article

The lack of the literature data on the actual CO₂ assimilation and dissimilation in aquatic plants under conditions of high chromium concentrations prompted this study to determine the efficiency of the photosynthetic apparatus and the actual rates of photosynthesis and respiration in Callitriche cophocarpa plants under chromium stress conditions. We hypothesized that C. cophocarpa would need to display an efficient acclimation mechanism that allows for efficient carboxylation and dark respiration in the presence of Cr(VI) ions. Shoots of C. cophocarpa plants were cultured in the control medium (Cr-free) and in the medium with addition of 0.1 mM potassium chromate. Results revealed that young and mature organs of examined plants respond differently to Cr(VI) ions. In young leaves, the decrease in pigment content (in comparison to control, car, chl a, total chl, and chl b by 15, 38, 39, and 49%, respectively) and distorted chloroplast ultrastructure led to lower efficiency of photosynthesis (by 22.5% compared to control). These leaves also exhibited reduced dark respiration efficiency (by 36.2% compared to control). In turn, mature leaves exhibited no change in photosynthesis and respiration efficiency. C. cophocarpa withstands Cr toxicity due to acclimation strategies associated with the reduction in the size of photosynthetic antennas and the effective use of reduced amounts of incoming radiation, as well as efficient dark respiration in mature leaves. Full article

Objective: The purpose of this study was to compare the influence of biological maturity status and training experience on motor performance in young athletes of different sport disciplines. Methods: Youth athletes (n = 84, 23 females) from five different sports (basketball, volleyball, track and field, wrestling, and badminton) participated in this study. Jump height was measured for the squat jump (SJ) and countermovement jump (CMJ). Peak torque during maximal voluntary contractions (MVCs) and torque steadiness at 20% MVC were assessed during plantar flexion (PF) and dorsiflexion (DF). Postural control was assessed with the one-leg test for both the right and left legs. K-means clustered analysis categorized participants into groups of low and high performers. Results: High performers had greater training experience than low performers for the SJ (p < 0.05), with no difference in maturity status (p > 0.05). Similarly, high performers had greater training experience (p < 0.05) than low performers for the CMJ, with no difference in maturity status (p > 0.05). High performers were more mature than low performers for MVC torque of DF (p < 0.001) and PF (p < 0.001), with no group differences in training experience (DF: p > 0.05; PF: p > 0.05). Maturity status for torque steadiness differed only for DF (p < 0.001), whereas there was no difference for PF (p > 0.05). There were no differences in either maturity status or training experience for one-leg-stance time (p > 0.05). Conclusions: These findings suggest that maturity status and training experience are linked to performance, although their relative roles differ across tasks. These findings reflect an interaction between biological maturity, training background and sports performance in youth athletes. Full article

From the witch-like widow on the edges of civilisation to the retired spinster who, after an active but disappointing sexual past, chooses to continue her single life, the celibate women of Edna O’Brien’s fiction have as much to contribute to the author’s career-long examination of the damage done by Irish patriarchy as any of the miserable housewives, resentful mothers, and abused girls who dominate critical analyses of her work. Unlike the many admirable nun characters in O’Brien’s fiction, the women in this study are not consciously renouncing society or deliberately retreating from the world. While they can be vulnerable characters who risk disapproval and even violence, they can also offer alternative models of Irish womanhood, subtle and complex, alternatives not always recognised when the narrator is a young girl and sometimes appreciated too late by more mature narrators and characters. Full article

Myocardial infarction remains a leading cause of mortality worldwide as the most severe clinical presentation of coronary artery disease, with an increasing trend in young adults. In the early phase of myocardial infarction, the mean blood pressure regulates the pressure distal to the occluded artery in the presence of well-developed collateral coronary circulation. Hypotensive medication administered after the myocardial infarction could compromise collateral recruitment and exacerbate myocardial ischemia. Collateral coronary circulation develops through angiogenic processes as a network of small blood vessels. After the myocardial infarction, the collateral arteries open and begin a process of arteriogenesis in order to mature into functional arteries. Although there are several well-known biochemical and molecular biomarkers for both myocardial infarction and angiogenesis, we need to associate these with arteriogenesis biomarkers in order to be able to fully determine the level of collateral coronary circulation development after myocardial infarction. In this review, we summarize some of the most important biomarkers that could provide insight into the collateral coronary arteriogenesis process. Our aim is to identify specific biomarkers that can be identified in the early processes of arteriogenesis after the myocardial event in order to quickly determine the best therapeutic strategy. Full article

Background/Objectives: Emerging evidence shows that dual tasking as well as the restriction of arm movements independently lead to detrimental effects on walking performance. However, it is unclear whether the deteriorations are more pronounced when applied together and if children (i.e., due to ongoing maturation processes) perform differently compared to young adults. This study investigated the influence of different arm movement strategies on subjective and objective markers related to beam walking under single-task (ST) and dual-task (DT) conditions in children and young adults. Methods: Twenty-six children (age: 11.3 ± 0.6 years) and 30 young adults (age: 23.2 ± 2.8 years) walked three meters on a balance beam with free and restricted (i.e., arms crossed over the chest) arm movements in a random order while concurrently performing a cognitive task (i.e., serial subtractions) or not. Walking outcomes (i.e., gait speed, cadence) were measured and used as objective markers. Self-reported task-related perceptions (i.e., balance confidence, fear of falling, perceived instability, conscious balance processing) were assessed and used as subjective indicators. Results: Walking under DT conditions (i.e., main effects of task) detrimentally influenced subjective task-related perceptions and walking outcomes, but using free arm movements (i.e., task × arm interactions) mitigated these deteriorations. Further, children exhibited largely stable levels of conscious balance processing, whereas young adults demonstrated overall higher levels along with pronounced differences between ST and DT walking when arm movements were unrestricted (i.e., group × task × arm interaction). Conclusions: These findings indicate that free arm movements seem to constitute a simple yet effective complementary ‘upper-body strategy’ that enhances postural control during a cognitively demanding walking task. Further, age differences imply that young adults compensate demanding walking conditions (i.e., DT walking with restricted arms) by elevated conscious processing of balance (i.e., a shift from automated to more conscious attention towards postural control). Full article

This study investigated interlimb asymmetries in lower limb performance using both vertical and horizontal jump tests in elite young basketball players. Specifically, it aimed to determine whether (1) unilateral jump performance and (2) the magnitude of interlimb asymmetry differed across maturity groups, whether (3) limb dominance influences performance, and whether (4) asymmetry direction is consistent across tests. One hundred elite male basketball players (U13 to U19) were categorised into three maturational stages: Pre-PHV (n = 19), Circa-PHV (n = 29), and Post-PHV (n = 52). Each athlete performed the following unilateral tests with both the dominant and non-dominant leg: single-leg hop, triple hop for distance, 6 m timed hop, single-leg countermovement jump (SL-CMJ), and single-leg drop jump (SL-DJ) from a 30 cm box. The Bilateral Strength Asymmetry (BSA) index was computed for each test. All tests showed significant differences between Pre-PHV and Circa-PHV groups (p < 0.001), whereas only the 6 m timed hop differed between Circa-PHV and Post-PHV (p < 0.01). BSA did not differ significantly across maturation stages in any test, except for the single-leg hop. Agreement in asymmetry direction between test pairs was slight to fair (kappa ≤ 0.29). BSA values remained largely stable across maturational stages, suggesting that interlimb asymmetries are established before PHV, likely during childhood. Limb dominance did not affect jump performance, and asymmetry direction varied between tests, confirming they are not interchangeable. Full article

Adolescence is a metabolically vulnerable period, during which rapid physiological maturation coincides with the dynamic remodelling of the gut microbiome. This narrative review summarises evidence from 2015 to 2025 to clarify how disturbances to the gut–liver axis driven by dysbiosis contribute to the development and progression of non-alcoholic fatty liver disease (NAFLD) in young people. Based on a systematic search of the databases PubMed, Scopus and Web of Science, we outline the basis of bidirectional communication between the gut and liver and emphasise how microbial imbalance alters the handling of lipids in the liver by enhancing de novo lipogenesis, impairing fatty acid oxidation and disrupting AMPK signalling and mitochondrial function. Consistent findings from clinical and experimental studies show that adolescents with NAFLD exhibit reduced microbial diversity, the enrichment of ethanol- and LPS-producing taxa, and altered short-chain fatty acid profiles. Each of these is associated with hepatic inflammation and metabolic reprogramming. Microbial molecules, including LPS, secondary bile acids and branched-chain amino acid metabolites, activate TLR4–NF-κB pathways, promote Kupffer cell activation and intensify oxidative stress. These mechanisms intersect with factors specific to adolescence, such as increased adiposity, hormonal shifts and diet-induced metabolic strain. Dietary patterns emerge as key modulators of these processes. Westernised diets promote dysbiosis and endotoxemia, whereas Mediterranean, fibre-rich and plant-based diets enhance SCFA production, strengthen epithelial integrity and modulate adiponectin-dependent hepatic metabolism. Micronutrient-sensitive epigenetic regulation, particularly that involving folate, choline and polyphenols, also plays a role in shaping lipid homeostasis and inflammatory tone. We also highlight emerging evidence that the activation of cytoprotective pathways, especially Nrf2, is dependent on lifestyle factors and links antioxidant-rich functional foods and physical activity to improved mitochondrial resilience and microbiome stability. We evaluate therapies targeting the microbiome, including probiotics, prebiotics, synbiotics and postbiotics, which reduce endotoxemia, restore microbial balance and complement dietary strategies. Thus, these findings emphasise the importance of age-specific, mechanistically informed interventions that integrate diet quality, microbial ecology, and the molecular pathways that govern metabolic health in adolescents with NAFLD. Full article

Background: Gut microbiome-derived metabolites, particularly short-chain fatty acids (SCFA) and tryptophan derivatives, are central mediators of the gut–brain axis. This ex vivo study assessed how nutritional interventions impact such metabolites during early life, a critical period for neurodevelopment. Methods: The effects of galacto-oligosaccharides (GOS), nutrient blends (vitamins, minerals and amino acids) and their combinations were evaluated in the gut microbiomes of infants (2–4 months, n = 6) and young children (2–3 years old, n = 6) using the ex vivo SIFR^® technology. Results: Baseline microbiome composition was age-dependent, with infants displaying lower α-diversity and greater interpersonal variability. After ex vivo incubation, nutrient blends increased the propionate/butyrate ratio and branched-chain fatty acids in young children and elevated several B-vitamins and amino acid-derived metabolites, including indole-3-carboxaldehyde, imidazoleacetic acid and pipecolinic acid. Combining nutrient blends with GOS exhibited potential synergistic effects on propionate (infants) and 2-hydroxyisocaproic acid (HICA, both age groups). GOS strongly stimulated Bifidobacteriaceae and increased metabolites linked to bifidobacterial metabolism like acetate, HICA, N-acetylated amino acids, aromatic lactic acids and acetylagmatine; in young children, butyrate and γ-aminobutyric acid (GABA) also increased. Conclusions: Combinations of GOS with nutrient blends impacted microbiome-derived metabolites associated with the gut–brain axis, with potential synergistic increases of metabolites with emerging roles in neurodevelopment, including GABA, acetylagmatine and HICA. Despite shared bifidogenic effects, differences between age groups indicate that microbiome maturity may influence responses to nutritional intervention. Future clinical studies are needed to determine whether these metabolite changes translate into neurodevelopmental benefits in vivo. Full article