Search Results (9)

Cautery disbudding is a painful procedure and major welfare concern in organic dairy production, which lacks effective and easily implemented methods of pain control. Willow bark (WB) has been used to treat chronic pain in humans, but there is no scientific evidence that WB has an analgesic effect in dairy cattle. The objective of this study was to evaluate the effect of an oral WB bolus on heart rate, salivary cortisol concentration, ocular temperature, and lying behavior (time, bouts, and bout duration) of 42 Holstein and crossbred calves. Heifer calves were born at the University of Minnesota West Central Research and Outreach Center, Morris, MN organic dairy. Calves were disbudded from 4 to 7 weeks of age (42 ± 3 d) and randomly assigned to one of three treatments (n = 14 calves/treatment): hot iron disbudding with lidocaine (LID; 5 mL per horn bud), hot iron disbudding with oral WB (200 mg/kg), or sham disbudding (SD). Saliva samples and thermal images were collected 1 h before disbudding, at disbudding, and 5 min, 10 min, and every 30 min until 240 min after disbudding. Cardiac monitors recorded heart rates continuously throughout the 5 h study period. HOBO loggers recorded lying behavior during the 72 h following disbudding. The variables included in the statistical model for analyses were fixed effects of treatment, time, and the interaction between treatment and time, and calf was a random effect. The mean heart rate of LID calves (123.3 ± 2.8 beats/minute) and WB calves (124.5 ± 3.2 beats/minute) were higher than SD calves (110.8 ± 2.3 beats/minute). Salivary cortisol concentrations were higher for the LID calves (103.8 pg/mL) and for the WB calves (103.4 pg/mL) than for the SD calves (85.5 pg/mL). The ocular temperature was not affected by treatment group. There were fewer lying bouts 24 to 48 h after disbudding compared to the first 24 h after disbudding in both the LID and WB treatment groups. Treatment group did not affect lying time or lying bout duration. Neither LID nor WB alleviated the pain of calves throughout the post-disbudding period. The results highlighted the need to discover suitable options for pain mitigation of calves in organic livestock production. Full article

The herbicide fluridone (1-methyl-3-phenyl-5-[3-trifluoromethyl (phenyl)]-4(1H)-pyridone) interferes with carotenoid biosynthesis in plants by inhibiting the conversion of phytoene to phytofluene. Fluridone also indirectly inhibits the biosynthesis of abscisic acid and strigolactones, and therefore, our study indirectly addresses the effect of reduced ABA on the roots and leaf buds development in stem cuttings of Salix babylonica L. ‘Tortuosa’. The stem cuttings were kept in distilled water (control) or in a solution of fluridone (10 mg/L) in natural greenhouse light and temperature conditions. During the experiments, morphological observations were carried out on developing roots and leaf buds, as well as their appearance and growth. After three weeks of continuous treatments, adventitious roots and leaf buds were collected and analysed. Identification and analysis of anthocyanins were carried out using micro-HPLC-MS/MS-TOF, while HPLC-MS/MS was used to analyse phenolic acids, flavonoids and salicinoids. The fluridone applied significantly inhibited root growth, but the number or density of roots was higher compared to the control. Contents of salicortin and salicin were several dozen times higher in leaf buds than in roots of willow. Fluridone increased the content of salicortin in roots and leaf buds and declined the level of salicin in buds. Fluridone also declined the content of most anthocyanins in roots but enhanced their content in buds, especially cyanidin glucoside, cyanidin galactoside and cyanidin rutinoside. Besides, fluridone markedly decreased the level of chlorophylls and carotenoids in the leaf buds. The results indicate that applied fluridone solution reduced root growth, caused bleaching of leaf buds, and markedly affected the content of secondary metabolites in the adventitious roots and leaf buds of S. babylonica stem cuttings. The paper presents and discusses in detail the significance of fluridone’s effects on physiological processes and secondary metabolism. Full article

Salix babylonica L. is a species of willow tree that is widely cultivated worldwide as an ornamental plant, but its medicinal resources have not yet been reasonably developed or utilized. Herein, we extracted and purified the total flavonoids from willow buds (PTFW) for component analysis in order to evaluate their in vitro anti-tumor and hypoglycemic activities. Through Q-Orbitrap LC-MS/MS analysis, a total of 10 flavonoid compounds were identified (including flavones, flavan-3-ols, and flavonols). The inhibitory effects of PTFW on the proliferation of cervical cancer HeLa cells, colon cancer HT-29 cells, and breast cancer MCF7 cells were evaluated using an MTT assay. Moreover, the hypoglycemic activity of PTFW was determined by investigating the inhibitory effects of PTFW on α-amylase and α-glucosidase. The results indicated that PTFW significantly suppressed the proliferation of HeLa cells, HT-29 cells, and MCF7 cells, with IC50 values of 1.432, 0.3476, and 2.297 mg/mL, respectively. PTFW, at different concentrations, had certain inhibitory effects on α-amylase and α-glucosidase, with IC50 values of 2.94 mg/mL and 1.87 mg/mL, respectively. In conclusion, PTFW at different doses exhibits anti-proliferation effects on all three types of cancer cells, particularly on HT-29 cells, and also shows significant hypoglycemic effects. Willow buds have the potential to be used in functional food and pharmaceutical industries. Full article

The present study was designed to evaluate the chemical extraction, chemical composition, and antioxidant and antibacterial properties of the total flavonoids in Willow Buds (TFW). We investigated the optimal extraction of TFW using response surface methodology (RSM). Chemical compounds were analyzed using Q-Orbitrap LC–MS/MS. The DPPH radical scavenging capacity, hydroxy radical inhibitory ability, and superoxide anion radical inhibitory ability were explored to determine the antioxidant properties of flavonoid extractions. The antibacterial effect was assessed via minimal inhibitory concentration. The results demonstrated that the optimal extraction conditions were an ethanol concentration of 50%, a time of 35 min, and a liquid/material ratio of 70:1 mL/g. Under these conditions, the yield of TFW was 7.57%. Eight flavonoids, a phenolic glycoside, and an alkaloid were enriched in the Willow Buds. The TFW exhibited significant antioxidant activity, with IC₅₀ values of 0.18-0.24 mg/mL and antimicrobial activity against Escherichia coli, Salmonella enterica, Staphylococcus aureus, and Streptococcus pneumoniae. TFW may be explored as potential and natural compounds in food and pharmacological applications. Full article

The major disadvantage of setting up a willow coppice is the low survival rate, which reduces economic efficiency and crop sustainability. The aim of this research was to test, under controlled conditions, the impact of water, gibberellic acid A3 (0.05%), and humic acid (0.2%) on the growth and development of two willow clones. Under humic acid treatment, 20 cm cuttings of the Tordis clone developed up to 15 roots, and 25 cm cuttings developed more than 23. In comparison, water stimulated more than 15 roots for both 20 and 25 cm cuttings. Gibberellins acted as an inhibitor, especially on the roots, and the cuttings dried out from the top to the middle, with weak development of shoots and callus formation. For both clones, the highest number of active buds was observed on 20 and 25 cm cuttings grown in water, with more than four for Inger and more than seven for Tordis. Root development of the Inger clone had a maximum of eight for 25 cm cuttings grown in water; it was three times lower in the same variant of Tordis and two times lower for the Tordis clone with humic acid treatment. In general, Inger cuttings of 15 and 25 cm highlighted a delayed root formation when humic acids and gibberellins were applied. In controlled condition experiments, the Tordis clone was more suitable owing to its higher development and increased growth stability. Full article

Ten willow cultivars grown in experimental plots were evaluated for performance, attractiveness to foragers, and the content and composition of soluble carbohydrates. The survival of willow cuttings in a thicket and in browse plots differed subject to cultivar, soil quality, and soil moisture content. The number of stump sprouts varied considerably, from 1.1 shoots in the weakest soils in Słonin, Poland, to 3.43 in the plot in Czempin, Poland. Browse plots were established in 2017. They were cut, and fencing was removed in early spring of 2019. Young shoots (10 cm shoot tip with buds, preferably eaten by animals) were sampled for analyses of soluble carbohydrates as potential attractors for foraging cervids. All willow cultivars contained the same soluble carbohydrates: glucose, fructose, galactose, sucrose, myo-inositol, galactinol, and raffinose. Total carbohydrate content ranged from 21.31 (S. amygdalina 1045) to 69.37 mg/g⁻¹ DM (dry matter) (S. purpurea). Glucose was the predominant soluble sugar in the shoots of all willow cultivars, excluding S. viminalis. The fructose content of the shoots was approximately twice lower than their glucose content in all willow cultivars. Smaller differences were observed in the content of myo-inositol, which ranged from 4.61 (S. amygdalina 1045) to 8.26 mg/g⁻¹ DM (S. fragilis cv. Kamon/Resko). The phloem of all willow species contained small quantities of galactinol and trace amounts of raffinose. Weak negative correlations were noted between total carbohydrate content, the content of glucose, fructose, and galactose vs. the attractiveness of willow shoots to foraging cervids. The remaining carbohydrates that occurred in smaller quantities in willow shoots were not correlated with their attractiveness to cervids. Full article

Shoot branching is regulated by axillary bud activities, which subsequently grow into branches. Phytohormones play a central role in shoot branching control, particularly with regard to auxin, cytokinins (CKs), strigolactones (SLs), and gibberellins (GAs). To further study the molecular basis for the shoot branching in Salix matsudana, how shoot branching responds to hormones and regulatory pathways was investigated, and potential genes involved in the regulation of shoot branching were identified. However, how these positive and inhibitory processes work on the molecular level remains unknown. RNA-Seq transcriptome expression analysis was used to elucidate the mechanisms underlying shoot branching. In total, 102 genes related to auxin, CKs, SLs, and GAs were differentially expressed in willow development. A majority of the potential genes associated with branching were differentially expressed at the time of shoot branching in S. matsudana, which have more number of branching. These findings are consistent with the growth and physiological results. A regulatory network model was proposed to explain the interaction between the four hormones that control shoot branching. Collectively, the results presented here contribute to a more comprehensive understanding of the hormonal effects on shoot branching in S. matsudana. In the future, these findings will help uncover the interactions among auxin, SLs, CKs, and GAs that control shoot branching in willow, which could help improve plant structures through the implementation of molecular techniques in targeted breeding. Full article

DNA methylation, an important epigenetic modification, regulates the expression of genes and is therefore involved in the transitions between floral developmental stages in flowering plants. To explore whether DNA methylation plays different roles in the floral development of individual male and female dioecious plants, we injected 5-azacytidine (5-azaC), a DNA methylation inhibitor, into the trunks of female and male basket willow (Salix viminalis L.) trees before flower bud initiation. As expected, 5-azaC decreased the level of DNA methylation in the leaves of both male and female trees during floral development; however, it increased DNA methylation in the leaves of male trees at the flower transition stage. Furthermore, 5-azaC increased the number, length and diameter of flower buds in the female trees but decreased these parameters in the male trees. The 5-azaC treatment also decreased the contents of soluble sugars, starch and reducing sugars in the leaves of the female plants, while increasing them in the male plants at the flower transition stage; however, this situation was largely reversed at the flower development stage. In addition, 5-azaC treatment decreased the contents of auxin indoleacetic acid (IAA) in both male and female trees at the flower transition stage. These results indicate that hypomethylation in leaves at the flower transition stage promotes the initiation of flowering and subsequent floral growth in Salix viminalis, suggesting that DNA methylation plays a similar role in vegetative–reproductive transition and early floral development. Furthermore, methylation changes during the vegetative–reproductive transition and floral development were closely associated with the biosynthesis, metabolism and transportation of carbohydrates and IAA. These results provide insight into the epigenetic regulation of carbohydrate accumulation. Full article

The production and feasibility of Short Rotation Coppice depend on cutting early performance. The shoot and root biomass production of Salix cuttings in hydroponic conditions was studied. The amount of sprouted biomass after four weeks of growth depended on cutting the diameter, but the original position of the cutting along the rod or number of visible buds was not in correlation with biomass produced. Application of mineral fertilizer or soil originating from the willow plantation did not increase the total production. On the contrary, the addition of soil tended to decrease biomass production and we assumed this was a result of a shortage of light. Under the influence of fertilization, plants allocated greater biomass to roots. Comparison of different clones revealed that those with S. dasyclados genes tended to allocate less biomass to roots and the poorest-performing clone in our experiment, also had the lowest wood production in the plantation. The number of visible buds on the cutting was also clone-specific. Full article