Search Results (598)

Most research on domestic dog (Canis familiaris) behavior has focused on pets with restricted movement. However, free-ranging dogs exist in diverse cultural contexts globally, and their interactions with humans are less understood. Tourists can facilitate unrestricted dog movement into wilderness areas, where they may negatively impact wildlife. This study investigated which stimuli—namely, voice, touch, or food—along with inherent factors (age, sex, sociability) motivate free-ranging dogs to follow a human stranger. We measured the distance (up to 600 m) of 129 free-ranging owned and stray dogs from three villages in southern Chile as they followed an experimenter who presented them one of the above stimuli or none (control). To evaluate the effect of dog sociability (i.e., positive versus stress-related or passive behaviors), we performed a 30 s socialization test (standing near the dog without interacting) before presenting a 10 s stimulus twice. We also tracked whether the dog was in the company of other dogs. Each focus dog was video-recorded and tested up to three times over five days. Generalized linear mixed-effects models revealed that the food stimulus significantly influenced dogs’ motivation to follow a stranger, as well as a high proportion of sociable behaviors directed towards humans and the company of other dogs present during the experiment. Juveniles tended to follow a stranger more than adults or seniors, but no effects were found for the dog’s sex, whether an owner was present, the repetition of trials, the location where the study was performed, or for individuals as a random variable. This research highlights that sociability as an inherent factor shapes dog–stranger interactions in free-ranging dogs when food is given. In the context of wildlife conservation, we recommend that managers promote awareness among local communities and tourists to avoid feeding dogs, especially in the context of outdoor activities close to wilderness. Full article

Antimicrobial resistance (AMR) is a growing global health threat, with wild birds increasingly recognized as potential reservoirs of resistant pathogens and as sentinels of environmental AMR. This study investigated the occurrence and AMR profiles of Gram-negative bacteria isolated from wild birds that died at the Wildlife Rescue Center in Vanzago, Lombardy, in 2024. Cloacal swabs were collected from 112 birds representing various ecological categories. A total of 157 Gram-negative bacteria were isolated and identified, including clinically relevant genera and species, such as Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., Salmonella spp., Pseudomonas aeruginosa, and Acinetobacter baumannii. Antimicrobial susceptibility testing revealed resistance to first-line and critically important antimicrobials, including those exclusively authorized for human use. Notably, a phenotype compatible with Extended-Spectrum Beta-Lactamase (ESBL) production was detected in four out of ten (40%) K. pneumoniae isolates. In addition, 20 out of the 157 (12.7%) isolated bacteria phenotypically exhibited a resistance profile indicative of AmpC beta-lactamase (AmpC) production, including Enterobacter spp. and P. aeruginosa. Resistance patterns were particularly interesting in birds with carnivorous, scavenging, or migratory-associated behaviors. These findings highlight the role of wild birds in the ecology and dissemination of antimicrobial-resistant bacteria (ARB) and highlight the need for wildlife-based AMR monitoring programs as part of a One Health approach. Full article

This paper presents field investigations of a corrugated steel soil–steel arch structure with a span of 25.7 m and a rise of 9.0 m—currently the largest single-span structure of its kind in Europe. The structure, serving as a wildlife crossing along the DK16 expressway in northeastern Poland, was constructed using deep corrugated steel plates (500 mm× 237 mm) made from S315MC steel, without additional reinforcements such as stiffening ribs or geosynthetics. The study focused on monitoring the structural behavior during the critical backfilling phase. Displacements and strains were recorded using 34 electro-resistant strain gauges and a geodetic laser system at successive backfill levels, with particular attention to the loading stage at the crown. The measured results were compared with predictions based on the Swedish Design Method (SDM). The SDM equations did not accurately predict internal forces during backfilling. At the crown level, bending moments and axial forces were overestimated by approximately 69% and 152%, respectively. At the final backfill level, the SDM underestimated bending moments by 55% and overestimated axial forces by 90%. These findings highlight limitations of current design standards and emphasize the need for revised analytical models and long-term monitoring of large-span soil–steel structures. Full article

Porcine circovirus type 3 (PCV3) has been detected in wild boars across many countries in Europe, Asia, and South America. However, data regarding the presence of porcine circoviruses in wild boars and ticks remain limited. In this study, we investigated the presence and genetic characteristics of PCV3 in wild boars and parasitizing ticks in Jiangsu, China. Samples, including whole blood, serum, tissues, feces, and oral fluids from wild boars, as well as ticks collected from 47 wild boars, were obtained between March 2021 and November 2022. PCR results indicated that 34.0% (16/47) of wild boars tested positive for PCV3, while ELISA detected 41.9% (18/43) seropositivity. RT-qPCR results showed that 7.2% (6/83) were positive for PCV3 in 83 analyzed tick samples, with all positive samples identified as Amblyomma testudinarium. The PCV3 genome obtained from wild boars was classified as PCV3a and was closely related to the strain identified in domestic pigs in Nanjing, Jiangsu Province. Collectively, these findings confirm the presence of PCV3 in wild boars in Jiangsu and suggest a possible link of PCV3 infection among domestic pigs, wild boars, and ticks, providing new insights into the transmission risk of PCV3 at wildlife–livestock–human interfaces and highlighting the genetic homology between strains from wild and domestic pigs. Full article

The ecological drivers of wildlife crop-foraging behavior—whether as a compensatory response to natural resource scarcity or as opportunistic exploitation of anthropogenic food sources—remain poorly understood in human–wildlife conflict research. Traditional methodologies, which primarily rely on direct observation and morphological identification, have limitations in comprehensively quantifying wildlife dietary composition, particularly in accurately distinguishing between morphologically similar plant species and conducting precise quantitative analyses. This study utilized DNA metabarcoding technology (rbcL gene markers) to identify and quantify plant dietary components through fecal sample analysis, systematically investigating the dietary composition and patterns of agricultural resource utilization of wild rhesus macaques (Macaca mulatta) in human–wildlife interface zones of southwestern China. A total of 29 rhesus macaque fecal samples were analyzed (15 from spring and 14 from winter), identifying 142 plant genera, comprising 124 wild plant genera, and 18 crop genera. The results revealed distinct seasonal foraging patterns: crops accounted for 32.11% of the diet in winter compared to 7.66% in spring. Notably, rhesus macaques continued to consume crops even during spring when wild resources were relatively abundant, challenging the traditional hypothesis driven by resource scarcity and suggesting that crop-foraging behavior may reflect an opportunistic, facultative resource selection strategy. This study demonstrates the significant value of DNA metabarcoding technology in wildlife foraging behavior research, providing scientific evidence for understanding human–primate conflict ecology and developing effective management strategies. Full article

Amdoparvoviruses, encompassing the well-characterized Aleutian mink disease viruses (AMDV) as well as less investigated viruses infecting both captive and wild animals, are important carnivoran viruses that are significant pathogens in the mink farming industry. We investigated the molecular epidemiology of amdoparvoviruses among Danish wildlife. Spleen samples from 118 animals of seven carnivoran species were screened with a pan-amdoparvovirus PCR, and the identified viruses were molecularly characterized. In one of five European badgers (Meles meles), we identified an AMDV-3 strain whose ancestors were likely of farmed mink origin. This virus was last reported on a mink farm in 2002, demonstrating how farm-derived viruses have established themselves among wildlife. We also discovered and fully characterized a novel virus found in five of 81 (6.2%) foxes (Vulpes vulpes) and one of five badgers (20.0%), which we named fox and badger amdoparvovirus 1 (FBAV-1). FBAV-1 fulfills the criteria for classification as a novel species and phylogenetically is positioned as an intermediate between the North American and Eurasian amdoparvoviral clades. This study provides baseline data and expands our understanding of amdoparvoviral ecology. Further studies including more animals across diverse geographic areas are warranted to clarify amdoparvovirus epidemiology, spread, cross-species transmission, epidemic potential, and evolutionary paths. Full article

The non-traditional companion animal (NTCA) sector, particularly involving avian species, has significantly expanded in Italy, raising concerns over the spread of infectious diseases. These animals can harbor various pathogens and act as reservoirs, posing risks to native wildlife through legal or illegal trade, escapes, or intentional releases. However, the epidemiology of avian pathogens in NTCAs remains poorly understood and is typically investigated only in symptomatic individuals. In the present study, cloacal and choanal cleft swabs were collected from 319 ornamental and raptor birds across 19 families, pooled and tested for beak and feather disease virus (BFDV), avian polyomavirus (APV-1), psittacid herpesvirus 1 (PsHV-1), and avian metapneumovirus (aMPV). BFDV and APV-1 were detected in 13.79% and 2.19% of birds, respectively, with five co-infections. No cases of PsHV-1 or aMPV were found. Both viruses showed a higher prevalence than in previous Italian and most of international studies, with several non-psittacine species, including birds of prey, testing positive—some for the first time. Mixed-species settings and participation in public exhibitions were proven as significant infection risk factors. The study highlights the growing relevance of BFDV and APV-1 in non-commercial birds and recommends improved biosecurity and preventive screening to reduce disease spread and safeguard animal health. Full article

In order to investigate the infection status of Cryptosporidium in O. curzoniae and B. grunniens in Zoige County, Sichuan Province, fecal samples from B. grunniens and gastrointestinal contents from captured O. curzoniae were collected between March and December 2023 from five townships (Dazhasi, Axi, Hongxing, Tangke, and Maixi). Genomic DNA was extracted, and nested PCR targeting the small subunit (SSU) rRNA gene of Cryptosporidium was performed. PCR-positive products were sequenced, trimmed, aligned, and subjected to phylogenetic analysis to determine species and genotypes. A total of 242 samples were obtained, of which 20 were Cryptosporidium SSU rRNA-positive, yielding an overall detection rate of 8.3% (20/242). The detection rates of O. curzoniae and B. grunniens were 7.0% (8/114) and 9.4% (12/128), respectively. Among the five sampling sites, Maixi town exhibited the highest detection rate (32.4%, 11/44), followed by Hongxing town (15.2%, 7/46) and Tangke town (4.6%, 2/44). Phylogenetic analysis detected an unidentified Cryptosporidium sp. in O. curzoniae, while C. bovis (n = 10) and C. ryanae (n = 2) were detected in B. grunniens. These findings demonstrate that Cryptosporidium infections are present in both O. curzoniae and B. grunniens in Zoige County, with notable differences in infection rates and species composition. Continued surveillance of Cryptosporidium in local livestock and wildlife is warranted to provide critical data for regional public health management. Full article

Rodents are the main wildlife species in the Dongting Lake area and can even cause significant biological disasters. In order to investigate the relationship between rodent communities and vegetation, the relationship between rodent population characteristics and vegetation cover between 2000 and 2020 was analyzed. The results showed that the rodent community in Dongting Lake has obvious spatial and temporal variability, and that Microtus fortis and Apodemus agrarius are currently the main rodent species in the area, with a higher population density in eastern Dongting Lake. Meanwhile, rodent fatness varied significantly between seasons, with the highest rodent fatness in winter at most survey sites. There was also significant spatial and temporal variability in vegetation cover, and the overall trend was increasing. There was a significant relationship between vegetation cover and rodent population density and fatness, and the relationship varied by rodent species. Microtus fortis population density was significantly correlated with medium to high levels of vegetation cover, but fatness was significantly correlated with low to medium levels of vegetation cover. The relationship between population density, fatness, and vegetation cover of A. agrarius is opposite to that of M. fortis. The study confirms that M. fortis is still the main pest rodent species in the Dongting Lake area, but the monitoring of A. agrarius and vegetation cover should be strengthened. Full article

Gastrointestinal (GI) parasitic infections in non-human primates are of growing concern due to their implications for both veterinary and public health. Long-tailed macaques (Macaca fascicularis), commonly found in peri-urban and temple environments in Southeast Asia, may act as reservoirs for zoonotic parasites, posing risks to humans and domestic animals. This study investigated the prevalence and species diversity of GI parasites in free-ranging macaques from four provinces in Northeast Thailand (Loei, Khon Kaen, Bueng Kan, and Sisaket). A cross-sectional study was conducted between April and May 2025. A total of 445 fecal samples were examined using two parasitological techniques: agar plate culture (APC) and the formalin–ethyl acetate concentration technique (FECT). The overall prevalence of parasitic infection was 86.5%, with Strongyloides sp. (65.2%) as the most prevalent helminth and Balantioides coli-like (29.5%) and Entamoeba histolytica-like (28.8%) as the predominant protozoa. Other parasites identified included helminths (Trichuris sp., Ascaris sp.) and protozoa (Blastocystis sp., Iodamoeba bütschlii, Entamoeba coli, and Chilomastix mesnili). Mixed infections were frequently observed, with both helminths and protozoa co-occurring in 37.3% of cases. The high infection rates and parasite diversity reflect substantial environmental contamination and sustained transmission cycles. These findings underscore the importance of integrated surveillance in wildlife populations and the need for One Health-based approaches to minimize zoonotic transmission risks at the human–animal–environment interface. Full article

Farmers face increasing pressure to maintain vital populations of the critically endangered field hamster (Cricetus cricetus) while managing crop damage caused by field mice. This challenge is linked to the UN Sustainable Development Goals (SDGs) 2 and 15, addressing food security and biodiversity. Consequently, the reliable detection of hamster activity in agricultural fields is essential. While remote sensing offers potential for wildlife monitoring, commonly used RGB imagery has limitations in detecting small burrow entrances in vegetated areas. This study investigates the potential of drone-based Light Detection and Ranging (LiDAR) data for identifying field hamster burrow entrances in agricultural landscapes. A geostatistical method was developed to detect local elevation minima as indicators of burrow openings. The analysis used four datasets captured at varying flight altitudes and spatial resolutions. The method successfully detected up to 20 out of 23 known burrow entrances and achieved an F1-score of 0.83 for the best-performing dataset. Detection was most accurate at flight altitudes of 30 m or lower, with performance decreasing at higher altitudes due to reduced point density. These findings demonstrate the potential of UAV-based LiDAR to support non-invasive species monitoring and habitat management in agricultural systems, contributing to sustainable conservation practices in line with the SDGs. Full article

The expansion of human activities can degrade natural habitats, thereby increasing threats to wildlife conservation. The wild populations of many species have declined due to the modification of natural habitats by humans. The Arabian wolf (Canis lupus arabs) is a subspecies of the gray wolf that is of conservation concern across its distribution range. The Arabian wolf is understudied in certain habitats (e.g., mountainous areas), which limits understanding of its overall ecology. Given its vulnerable conservation status, this study aimed to collect relevant data and information on incidents and potential threats facing this predator in the rugged mountainous habitats of western Tabuk province, Saudi Arabia, and how the effects of these threats can be minimized. In these mountain habitats Arabian wolves encounter various severe threats that challenge relevant conservation efforts. Observations of such threats—some of which result in wolf mortality—represent serious challenges to the survival of wild Arabian wolves. Conflicts with humans and livestock represent considerable threats that must be appropriately managed. Additionally, the potential association between Arabian wolves and free-ranging dogs requires further investigation. Various conservation scenarios and mitigation approaches can be applied to help reduce negative impacts on Arabian wolf populations and maximize their likelihood of survival. Overall, ensuring the persistence of such a unique desert-adapted apex predator in this ecosystem must become a conservation priority. Full article

Background: The illegal trade in wildlife remains a critical threat to biodiversity, prompting the development of international regulatory frameworks such as the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). One of the key challenges in enforcement is the detection of CITES-listed species in highly processed consumer goods. Methods: This study investigates the use of molecular techniques to detect animal DNA in two selected commercially available medicinal products—a balm and a gel—marketed with ingredients suggestive of protected species such as the brown bear (Ursus arctos) and the medicinal leech (Hirudo medicinalis). Results: Although DNA from these target species was not detected, the analysis revealed the presence of genetic material from the American mink (Neovison vison) and domestic pig (Sus scrofa), indicating the undeclared use of animal-derived substances. While limited in scope, these findings suggest potential ethical and transparency concerns, particularly for consumers adhering to vegetarian, vegan, or religious dietary practices. Conclusions: The study illustrates the feasibility of applying DNA-based screening methods in complex, degraded matrices and their potential as supportive tools in consumer product assessment. However, broader studies are necessary before drawing general regulatory or conservation conclusions. Full article

Sound source localization represents one of the major challenges for soundscapes due to the dynamicity of a large variety of signals. Many applications are found related to ecosystems to study the migration process of birds and animals other than other terrestrial environments to survey wildlife. Other applications on sound recording are supported by sensors to detect animal movement. This paper deals with the immersive 3D soundscape by using a multi-channel spherical microphone probe, in combination with a 360° camera. The soundscape has been carried out in three Italian squares across the city of Parma. The acoustic maps obtained from the data processing detect the directivity of dynamic sound sources as typical of an urban environment. The analysis of the objective environmental parameters (like loudness, roughness, sharpness, and prominence) was conducted alongside the investigations on the historical importance of Italian squares as places for social inclusivity. A dedicated listening playback is provided by the AGORA project with a portable listening room characterized by modular unit of soundbars. Full article

Wildlife serves as a significant reservoir for various pathogens transmissible to domestic animals and humans. Vector-borne diseases represent an increasing concern in Europe, affecting both animal and human health. This pilot study investigated the circulation of endemic and emerging vector-borne viruses in wild ungulates in Hungary, utilizing a One Health approach. Serum samples were obtained from European fallow deer (Dama dama), red deer (Cervus elaphus), and roe deer (Capreolus capreolus) during routine national game management activities between 2020 and 2023. Samples were analyzed for antibodies against the Bluetongue virus (BTV), West Nile virus (WNV), and Epizootic hemorrhagic disease virus (EHDV) using ELISA and neutralization tests. The results revealed a WNV seroprevalence of 22.3% in fallow deer and 31.8% in red deer, while BTV seroprevalence was 2.5% in fallow deer. All samples were negative for EHDV antibodies. These findings confirm the circulation of WNV and BTV in Hungarian wild ungulates. While the study’s design precludes statistical analysis due to non-random sampling, it demonstrates the potential of integrating wild ungulate serosurveillance into disease monitoring programs, leveraging established wildlife management activities for a cost-effective and complementary approach to One Health surveillance, particularly considering the ongoing spread of EHDV in Europe and the importance of BTV serotype monitoring for effective vaccination strategies. Full article