Search Results (1,622)

The increasing demand for sustainable agriculture has intensified interest in beneficial microbes as eco-friendly alternatives to chemical pesticides for plant disease control. Among these, plant growth-promoting rhizobacteria (PGPR) have attracted great interest because they can suppress plant pathogens and strengthen plant health through molecular mechanisms. Recent studies suggest that PGPR protect plants from disease not only by directly attacking pathogens but also by changing how plant immune genes are expressed through epigenetic processes. This review brings together current knowledge on epigenetic regulation in plant–PGPR interactions, focusing on DNA methylation, histone modifications, and non-coding RNA pathways. PGPR colonization activates plant immune signaling through pattern recognition receptors, MAPK cascades, reactive oxygen species, and plant hormones. The review also covers the range of bacterial signals—including lipopolysaccharides, flagellin, cyclic lipopeptides, and volatile organic compounds—that prepare plant defenses, and explains how the recognition of these signals reshapes chromatin structure at defense genes. In addition, the review discusses how these changes may influence induced systemic resistance and examines emerging, though still limited, evidence on whether they could potentially be transmitted to subsequent generations. A better understanding of how microbial signals regulate host epigenetics may reveal new ways to improve plant immunity and balance growth with defense. Overall, available evidence indicates that PGPR-induced epigenetic changes represent a promising and environmentally friendly approach to crop protection; however, field-level validation and mechanistic confirmation in non-model crop species remain necessary before this strategy can be considered practically applicable. Full article

Longmeizi 龍眉子 was an inheritor of the Southern Lineage of Daoism 道教南宗 under Weng Baoguang 翁葆光. By tracing the historical documentation of Longmeizi’s Daoist lineage, it becomes evident that the narrative details were continuously enriched through textual accumulation. By tracing and analyzing the formative history of documents related to Longmeizi’s Daoist lineage, it is evident that in the process of forming this Daoist lineage, lineage identity 宗派認同 was continuously solidified and even “labeled 標籤化” within these layered texts. The transmission genealogy between patriarchs across generations gradually became clear, definite, and verifiable. After Longmeizi compiled the Jinye Huandan Yinzheng Tu 金液還丹印證圖 (Illustrations of the Return of the Liquified Gold to the Cinnabar Field) from the Jiading period (1208–1224) of the Southern Song Dynasty to the beginning of the Yuan Dynasty, this book was initially transmitted within the Daoist lineage: Longmeizi → Bai Yuchan 白玉蟾 → Wang Jinchan 王金蟾. By the end of the Yuan Dynasty, a literatus named Yuanyangzi Lin Jing 元阳子林静 from Wuxing 吴兴 had also read this book. During the Ming and Qing dynasties, the mode of transmission for the Jinye Huandan Yinzheng Tu shifted from being primarily transmitted orally within Daoist circles to being primarily disseminated through the printing and circulation of books. This led to the emergence of many different versions and commentaries of the Jinye Huandan Yinzheng Tu. Through the compilation and printing of book series, the Jinye Huandan Yinzheng Tu gained broad circulation during the Ming and Qing dynasties. Its annotators, publishers, and readers spanned various identities and social classes, while its geographic reach extended to the Central Plains (Zhongyuan 中原), Southwest China, and Jiangnan regions. By examining the textual circulation history of the Jinye Huandan Yinzheng Tu, it can be observed that the development of the book printing industry during the Ming and Qing periods, particularly the flourishing of series publications, facilitated a shift in the primary mode of transmission for Daoist texts and even in the nature of the texts themselves. On the other hand, the case study of the Jinye huandan yinzheng tu is an example that illustrates the diversity and richness in the methods of Daoist cultural transmission and their development during the Ming and Qing dynasties. Full article

Marine controlled source electromagnetic (CSEM) surveys have been proven to be an effective tool in hydrocarbon exploration, principally due to the method’s ability (in the right circumstances) to identify electrical resistivity contrasts between hydrocarbon-saturated and brine-saturated sedimentary units. However, the sensitivity of such surveys decreases in shallow water, for deeper targets, and for targets with limited horizontal extent. In principle, the resolution and sensitivity of a survey can be improved by moving either the transmitting or the receiving dipoles into the sub-surface. We have therefore investigated the sensitivity of Seafloor to Borehole CSEM (sbCSEM) survey geometries, specifically for the case of simplified targets with small lateral dimensions in shallow water areas—including targets whose depth of burial substantially exceeds their lateral extent. The results are encouraging. Neither small target size nor shallow water presents obstacles in principle to the use of this approach. Our models reveal distinct lobes in the patterns of electric field and current density amplitudes around a sub-seafloor transmitting dipole. The shape, positions and amplitudes of these lobes are all strongly modified by the presence of one or more small resistive targets, and they are strongly influenced by the positions of target edges. These effects significantly modify the pattern of electric fields at the seafloor and hence result in good sensitivity for realistic survey geometries. Small targets can be detected by seafloor receivers when the sub-seafloor transmitting dipole is located at some distance laterally outside the targets—leading to potential applications in ‘step-out’ prospecting. The asymmetry of responses at the seafloor from targets that are offset with respect to transmitter location has potential applications in field appraisal, while monitoring of reservoirs during production provides another possible application. Varying the depth of the transmitter down the borehole generates a Vertical EM Profiling (VEMP) survey—analogous to Vertical Seismic Profiling (VSP)—and we demonstrate that this too can have useful applications. Modelling for deeper (3 km sub-seafloor) targets continues to yield encouraging results and suggests that step-out sbCSEM may be effective at depths beyond the detection limit of conventional seafloor–seafloor CSEM. Full article

Wheat streak mosaic (WSM), historically attributed to wheat streak mosaic virus (WSMV) and transmitted by the wheat curl mite (WCM; Aceria tosichella), remains a major cause of yield loss in the Texas High Plains. In recent years, Triticum mosaic virus (TriMV), also transmitted by WCM, has emerged as an increasingly important component of the WSM disease complex. Under field conditions, TriMV is most frequently detected in mixed infections with WSMV. Management of WSM relies primarily on resistant cultivars carrying genes such as Wsm1 or Wsm2. Although synergistic interactions between WSMV and TriMV have been documented under controlled conditions, their dynamics during natural field infections—particularly during the latent phase between initial infection and symptom development—remain poorly understood. Moreover, the extent to which host genotype influences virus–virus interactions and vector acquisition dynamics in the field has not been fully resolved. Replicated field trials conducted over two growing seasons were used to quantify temporal accumulation patterns and relative ratios of WSMV and TriMV in susceptible (TAM 304) and resistant cultivars differing in resistance source (BT [Wsm1] and Joe [Wsm2]) under natural disease spread. WSMV remained the predominant virus in mixed infections across cultivars, sampling times, and disease stages. However, as plants aged and entered senescence, WSMV titers declined more rapidly than TriMV titers, resulting in a progressive reduction in the WSMV-to-TriMV ratio. From early infection through disease development, the Wsm1 cultivar (BT) consistently supported significantly lower TriMV accumulation than the Wsm2 cultivar (Joe), providing a mechanistic explanation for the comparatively stronger disease suppression associated with WSM. Mites feeding on BT also acquired lower TriMV titers. Although viral concentrations in wheat tissue were strongly correlated with those detected in feeding mites, substantial differences in plant-level WSMV-to-TriMV ratios among cultivars were not mirrored within the vector. These findings indicate that while host resistance regulates absolute virus accumulation, vector-associated factors may influence the relative proportions of viruses detected following acquisition, with important implications for WSM epidemiology and resistance deployment in field systems. Full article

Multi-baseline phase unwrapping is a critical procedure in interferometric synthetic aperture radar (InSAR) data processing, and cluster analysis (CA)-based algorithms have become an important research direction in this field. However, traditional CA algorithms suffer from cluster group loss, cluster centerline offset under high noise, and time-consuming search, leading to limited unwrapping performance. To address these issues, this article proposes a multi-baseline phase unwrapping algorithm based on the integrated processing of intercept pre-filtering and ambiguity number vector determination, achieving significant performance improvements through four core technical optimisations. First, the linear relationship model of ambiguity numbers is extended to be compatible not only with the traditional one-transmitter, multi-receiver architecture but also with distributed multi-baseline InSAR systems with independent transmit–receive links for each baseline. Second, through verification from both forward and reverse uniqueness perspectives, a strict one-to-one mapping relationship between reference intercepts and ambiguity number combinations is established and validated. Third, a double constraints screening strategy for ambiguity number combinations combining the single-baseline elevation range intersection constraint and the multi-baseline elevation space common intersection constraint is designed. Integrating the effective elevation range of the observation area, this strategy accurately filters out valid ambiguity number combinations with physical rationality, ensuring the reliability of the reference intercept vector. Fourth, an intercept pre-filtering method based on the reference intercept vector is proposed, which unifies actual intercept pre-filtering and ambiguity number vector determination. To verify the performance of the proposed algorithm, a simulation data experiment under varying noise levels and real data experiments are conducted. Results demonstrate that the algorithm can maintain intact cluster structures under complex noise conditions. It achieves a synergistic improvement in unwrapping accuracy and computational efficiency, and thus significantly outperforms comparative algorithms. The proposed algorithm achieves high precision and efficiency for multi-baseline InSAR processing in complex scenarios, with important application value in practical engineering. Full article

Pesticidal plants are vital for pest management in sub-Saharan Africa, yet knowledge about them is culturally and geographically unevenly documented. This study examined ethnobotanical knowledge and the distribution of pesticidal plants among six ethnic groups (Maasai, Chagga, Iraqw, Pare, Nguu, Zigua) in northern Tanzania. Data related to ethnobotanical knowledge were collected from questionnaire surveys involving 266 participants, 24 focus group discussions, 26 key informant interviews, complemented by field verifications across the six ethnic groups. Pesticidal plant coordinates were accessed from herbarium voucher specimens from The National Herbarium of Tanzania. Chi-square tests of independence assessed associations between ethnic groups and knowledge transmission pathways. Penalized logistic regression analysis was conducted to assess the influence of demographic factors on reported knowledge of pesticidal plants. Spatial overlay was conducted to examine the distribution of pesticidal plant species occurrences across agroecological zones and rainfall gradients. The results revealed a significant association between ethnic group and the source of pesticidal plant knowledge. Across all ethnic groups, knowledge was predominantly acquired through family/community traditional sources, with the highest frequencies recorded among the Maasai, Iraqw, and Zigua. Knowledge is mainly transmitted orally, particularly among the Maasai, Iraqw, and Zigua. A total of one hundred and six distinct species were recorded across the six ethnic groups surveyed, with Tephrosia vogelii and Solanum incanum being the most frequently cited. Leaves were the most frequently used plant part across all ethnic groups, with notably high usage among the Chagga, Iraqw, and Maasai. Perceptions of the declining population of pesticidal plants were the highest among the Maasai. Spatial mapping revealed pesticidal plant hotspots in the Northern Rift and Volcanic Highlands agroecological zones, and they fall within zones receiving moderate to relatively high rainfall. The findings highlight that ethnobotanical knowledge of pesticidal plants in northern Tanzania is strongly shaped by ethnic affiliation, oral knowledge transmission, and localized ecological availability, with clear spatial hotspots aligned to specific agroecological zones and high-rainfall areas. Full article

Background/Objectives: The Achilles tendon enthesis (ATE) is a key load-transmitting structure that is frequently affected in musculoskeletal disorders, including insertional tendinopathy, overuse injuries and inflammatory enthesopathies. Reliable non-invasive assessment of the enthesis structure is therefore of increasing clinical importance. This study evaluated the ability of advanced magnetic resonance (MR) microscopy to depict the ultrastructural organization of the ATE using histology as a reference standard. Methods: Five human ATEs from anatomical body donations were included. Two specimens were used for protocol development of the histological preparation, whereas three specimens underwent the full multimodal pipeline comprising undecalcified methyl methacrylate (MMA) thin-section histology with Giemsa staining, T2*-weighted 3D-variable echo time (vTE) MR microscopy at 7 Tesla, and microradiography. Results: Histological analysis demonstrated excellent preservation of fibrocartilage zones and mineralized interfaces. Corresponding MR microscopy data allowed the identification of major structural components of the enthesis, particularly mineralized regions, although fine ultrastructural details remained beyond the MR microscopy resolution. Microradiography supported interpretation of the mineralized tissue architecture and MR microscopy signal characteristics. Conclusions: These findings indicate that high-field MR microscopy can capture clinically relevant structural features of the Achilles tendon enthesis, while histology remains essential for detailed ultrastructural validation. The combined imaging approach provides a translational framework that may support improved diagnosis, monitoring and treatment evaluation in musculoskeletal disorders involving the osteotendinous junction. Full article

Laser beam combining is essential for achieving high-power and high-radiance output. However, atmospheric turbulence induces independent tip–tilt aberrations across discrete sub-beams in laser array systems, which severely degrades the concentration of far-field energy. Traditional wavefront sensing techniques are primarily designed for the continuous wavefront of a single laser and are not directly applicable to laser array, whereas indirect optimization-based methods often suffer from slow convergence and limited real-time performance. To address these limitations, this study introduces a tip–tilt aberration compensation system for laser array propagation based on cooperative beacons with a shared-aperture transmit–receive configuration. The primary innovation consists of a modified Shack–Hartmann wavefront sensor (SHWFS) tailored to a discrete multi-beam layout, which facilitates the direct, independent, and simultaneous measurement of tip–tilt aberrations for each sub-beam. In conjunction with a segmented deformable mirror (SDM), the architecture can facilitate real-time closed-loop correction with high bandwidth and high precision. Numerical simulations of a 7-, 19-, and 37-beam laser array, together with validation experiments utilizing a 30-beam configuration, demonstrate that the proposed approach effectively suppresses tip–tilt error induced by turbulence. After closed-loop correction, the Strehl ratio (SR) increases above 0.92 ($r_0=5\mathrm{cm}$), while the beam quality factor β reduces below 1.37 ($r_0=5\mathrm{cm}$). Furthermore, the system retains performance stability as the number of sub-beams increases, demonstrating the scalability of the proposed method. In contrast to conventional approaches designed for a continuous wavefront, the proposed method offers a feasible approach for a discrete laser array system, providing robust and scalable tip–tilt correction under varying atmospheric conditions. Full article

Toxoplasma gondii is an intracellular protozoan transmitted via environmentally resistant oocysts present in food and water, as well as through the consumption of meat containing infective bradyzoites. This study evaluated the inactivation of T. gondii oocysts and bradyzoites (ME-49 strain) by Pulsed Electric Field technology (PEF). Treatment efficacy was determined by mouse bioassay combining brain qPCR and indirect immunofluorescence (IFA), with complementary qPCR in Hs27 cells. The infectious dose (ID₅₀) of T. gondii was estimated at 34.6 oocysts. PEF-treated oocysts (15 kV/cm; 50 kJ/kg; 225 µs) showed a significant reduction in infectivity compared with untreated controls; accordingly, the dose required to establish infection increased to 85.3 oocysts after PEF treatment. Brain qPCR and IFA were highly correlated, whereas heart tissue was less sensitive. Bradyzoites recovered from PEF-treated meat (3.3 kV/cm; 27 kJ/kg; 1600 µs) showed a 50% infectivity reduction compared with untreated samples. In vitro assays confirmed an in vivo reduction in infectivity, indicating that cell cultures can serve as an ethical and efficient tool for preliminary viability assessment. This is the first evidence of T. gondii inactivation by PEF, highlighting its potential as a non-thermal strategy. Further studies are needed to optimize treatment parameters. Full article

Neglected tropical diseases (NTDs) such as soil-transmitted helminthiasis, lymphatic filariasis, schistosomiasis, leprosy, rabies, and food-borne trematodiasis are endemic in the Philippines. Despite global and national elimination efforts, these six NTDs remain a persistent burden to the poor, those living in Geographically Isolated and Disadvantaged Areas (GIDAs), and other vulnerable groups. This narrative review synthesized data from Field Health Services Information System (FHSIS) reports of the Philippine Department of Health (DOH) from 2020 to 2024, the available literature from electronic databases, and DOH and WHO reports focusing on the challenges, barriers, and gaps in NTD control and elimination in the country. Core challenges include complex epidemiological landscapes, lapses in disease surveillance, infrastructure, and fragmented health care systems. Gaps include access to diagnostics, insufficient funding and human resource training, and scarcity of local studies focusing on endemic NTDs. With these challenges and gaps, this review highlights the need for a real-time feedback loop system in surveillance strategy, community-based interventions, full integration of NTDs in primary health care, and collaboration between government, NGOs and private entities. Addressing these challenges and gaps is key to shifting from control to elimination. Full article

Objectives: This study aimed to demonstrate the feasibility of a novel wireless energy-transmitting implantable diaphragm pacemaker for restoring respiratory ventilation. Methods: The diaphragm pacing (DP) system was designed based on the principle of electromagnetic resonance coupling. The safety of device implantation was analyzed through finite-element simulations of multi-field coupling between electromagnetic heating and biological tissue. In vitro testing with coils embedded in pork demonstrated the system output characteristics. This device was used in miniature Bama pigs that underwent deep anesthesia and respiratory arrest (N = 8). Respiratory airflow, diaphragmatic displacement, and blood gases were used to evaluate the effectiveness of the designed DP system. Results: Thermal effect simulation results show that the temperature rise of the surrounding tissue does not exceed 2 °C during 1 h of transmission power (0.5–1.3 W) operation of the receiver. In vitro tests with two receivers embedded in pork showed that the DP system can effectively output stimulation waveforms over a certain transmission distance (5–35 mm). The stimulation waveform output by the receiver is consistent with the parameters set by the external controller. In phrenic nerve electrical stimulation experiments, the peak respiratory airflow and tidal volume remained stable over 50 consecutive respiratory cycles. The tidal volume (108.63 mL) and diaphragmatic displacement (0.883–2.15 cm) in a pig induced by DP demonstrate the effectiveness of respiratory ventilation. The arterial blood gas analysis results and temperature rise experiment during implantation further confirmed the effectiveness and safety of the ventilation. Conclusions: The implantable diaphragmatic pacemaker developed in this study exhibits good thermal safety, stable output, and effective respiratory ventilation. A control group with commercial diaphragmatic pacemakers and data from chronic implantation experiments are needed to further evaluate its effectiveness. Full article

Life evolved under a persistent 1 g field that is continuous, ubiquitous, and directionally structured. Here, we synthesize evidence across evolutionary biology, mechanobiology, and genome architecture to propose gravity as a mechanical boundary condition that helped canalize the emergence of complex multicellularity. Order-of-magnitude considerations indicate that gravity-derived hydrostatic loads can fall within force/pressure regimes relevant to nuclear and chromatin mechanosensitivity when transmitted through adhesion–cytoskeleton–LINC–lamina coupling. Comparative genomic and imaging frameworks suggest that complex animals increasingly rely on volumetric genome organization (packing domains and higher-order 3D architectures) that supports durable transcriptional memory and stable differentiated cell identities. Integrating these concepts with altered-gravity experiments, we argue that microgravity and hypergravity perturb chromatin topology and region-level transcription in rapid, largely reversible patterns consistent with a mechanically defined 1 g reference state. We advance a boundary-condition thesis: gravity is not a sole driver but a stable reference that likely contributed to the evolvability and long-term robustness of mechanogenomic architectures required for high-dimensional differentiation and tissue homeostasis. Full article

This research-related study examines the relevance of mud pulse telemetry (MPT) systems and their intersection with drilling performance, focusing on data transmission signal propagation performance and overall operation under different drilling parameters conditions, with an additional focus on drilling fluid flow rate and downhole pressure conditions. The novelty of this study lies in the investigation of adjustments to drilling operating parameters that could potentially improve the transmission of telemetry signals during drilling, in real time, without requiring mechanical or functional modifications to the MPT system itself. Improvements on transmission performance in situations where the data rate may be limited are also addressed, presenting an alternative through possible propagation velocity improvements to counterbalance it. A detailed chronological technical scientific literature review details important parts on analyses of pressure pulse propagation velocities focused on data transmission. A systematic experimental approach was developed and put into practice to evaluate the MPT systems in regard to tendencies on transmission performances, emphasizing pressure pulse propagation velocity. The laboratory-scale experiments were conducted at the Institute of Drilling Engineering and Fluid Mining (IBF) from the Technical University Bergakademie Freiberg (TUBAF), namely the Flow-loop Research Facility, to assess the impact of fluid flow rate (and subsequent pressure) on data transmission efficiency. Experimental results demonstrate that increasing the flow rate significantly speeds up signal propagation. In the performed experiments, for the mud siren configuration, increasing the flow rate from 15 to 25 m³/h improved the data transmission performance by approximately, at minimum, 18%, while for the positive mud pulse system, an increase in flow rate from 11.5 to 14 m³/h resulted in a propagation velocity rise of about 19%. The results also showed that higher concentrations of glycerin in the working fluid reduced the propagation velocity, confirming the influence of the fluid’s rheological properties on telemetry performance. At the end, in the presented case study, for 6 bps data rate configurations and for a transmission of a 40-bit string, it was demonstrated that the propagation time from downhole to the surface could potentially represent approximately 40% of the total time demanded for transmitting the desired information (generation plus propagation time). It was verified that an increment of 0.02208 m³/s (350 gpm) could lead to shortening eventual surveying procedures by 1–2 s, and that it could equally represent 1.137 bps. This is a relevant outcome, since, without any physical or functional alteration to the MPT system, one could have the data transmission performance improved, an approach not yet analyzed in the literature nor at the industrial park. These results, added to the detailed literature investigation and interaction with field personnel, indicate that the drilling fluid flow rate is a critical operational parameter affecting both the telemetry signal transmission speed and the overall drilling efficiency. Increasing the flow rate can reduce survey transmission time and decrease operational exposure to drilling hazards, such as drill string sticking. The results provide quantitative information applicable in optimizing measurement-drilling telemetry and help support the development of integrated drilling optimization strategies that balance drilling performance with real-time data transmission assurance in deep drilling operations. Full article

In response to the need to monitor groundwater migration and structural damage to rock strata during tunnel excavation and coal mining, this paper presents a novel electromagnetic detection system that features continuous ground-based transmission and full-waveform underground observation. As the transmitted waveform is crucial for determining the distribution of induced eddy currents and the characteristics of the secondary field response, studying these response characteristics is essential for the system’s practical application. This study selects four typical transmission waveforms—step, triangular, half-sine and trapezoidal—and uses a tetrahedral, three-dimensional grid discretization method to analyze the transient electromagnetic full-wave response patterns of electrical and magnetic sources under different waveform excitations. This elucidates the propagation characteristics of electromagnetic fields in the medium. The research reveals that the waveform type during energization significantly influences the electromagnetic response, with the full-wave response characteristics of electrical and magnetic sources differing significantly in the near-source region and response trends converging in the far-source region. In practical detection, combining the advantages of the three-component responses of the electrical and magnetic sources can effectively improve detection accuracy. The findings of this study provide important theoretical support for optimizing the design of transient electromagnetic detection systems and precisely interpreting detection data. They also lay a theoretical foundation for electromagnetic detection applications in fields such as mineral resource exploration and engineering geological surveys. Full article

To evaluate the performance of a printed log-periodic dipole antenna (PLPDA) in outdoor environments, we present unmanned aerial vehicle (UAV)-based antenna measurements conducted in the far-field region. Non-tethered UAV flight operations were achieved by configuring commercially available UAVs separately as a transmitter (TX) and as a receiver (RX). UAVs configured in non-tethered mode provide flexibility in terms of altitude maintained by the UAV from the ground level. The TX section of the UAV consists of a portable signal generator and a PLPDA configured to transmit signals with an output power of +15 dBm at 0.8 and 3.5 GHz. Similarly, the RX section of the UAV is equipped with a real-time spectrum analyzer and an identical PLPDA. Using these two UAVs in TX and RX modes, the radiation pattern of the PLPDA was obtained in the azimuth plane. Since two identical PLPDAs were used, the realized gain of the PLPDA is evaluated using the two-antenna gain method. The test scenario involved the TX UAV hovering at the center while the RX UAV followed a circular trajectory around it. A comparison between the UAV measurements, anechoic chamber measurements, and simulated data demonstrates good agreement, validating the reliability of the measurements. Full article