Search Results (1,263)

Introduction: Marine heatwaves are increasing in frequency and intensity, posing major challenges for fishes inhabiting shallow coastal ecosystems. Short-term exposure to extreme warming can alter metabolic performance and thermal tolerance, with potential consequences for species persistence and school composition in thermally variable habitats. Understanding the capacity of coastal fishes to withstand acute warming events is therefore essential for predicting ecological responses to climate change. Objective: We aimed to determine the effects of simulated marine heatwaves on thermal tolerance and metabolic performance in juvenile grey mullets, Chelon labrosus and Chelon aurata, two abundant sympatric species inhabiting the Ria Formosa lagoon (southern Portugal). Methodology: Juvenile mullets acclimated at 17 °C were exposed to simulated heatwave treatments of 23, 27, or 33 °C and sampled either at peak temperature or after 48 h and 1-week recovery at 17 °C. Critical thermal maximum (CTmax, using a 1 °C/min thermal ramp), static oxygen consumption (MO2), and intermittent respirometry parameters were measured. Standard metabolic rate (SMR), maximum metabolic rate (MMR), and aerobic scope (AS) were derived from intermittent respirometry. A complementary temperature-ramp (>3 h at each temperature step 17, 23, 27 and 33 °C) was performed to evaluate routine metabolic rate and estimate Q10 values across increasing temperatures. Additional plasma and tissue analyses are being conducted to assess energetic substrate mobilization and cellular responses to thermal and oxidative stress. Results: CTmax increased significantly with warming in both treatment modes, demonstrating rapid heat hardening in juvenile mullets. Fish exposed to 27 and 33 °C exhibited higher CTmax than control fish, and this elevated tolerance persisted after recovery. Chelon labrosus showed slightly higher CTmax values than C. aurata. Oxygen consumption increased with temperature, with the strongest responses occurring at 33 °C. SMR increased markedly with warming, particularly in heatwave-exposed fish, while MMR increased mainly at the highest temperature treatment. In contrast, AS showed no clear thermal optimum or decline across treatments. Routine metabolic rate increased non-linearly with temperature in the complementary ramp experiment, with a mean Q10 of 2.28, confirming strong thermal dependence of metabolism. Conclusions: Juvenile mullets possess substantial short-term thermal plasticity and can rapidly increase heat tolerance during marine heatwaves but this enhanced tolerance is accompanied by elevated metabolic costs under extreme warming, indicating potential energetic trade-offs near upper thermal limits. Differential physiological responses between species may influence school composition and ecological performance across thermal landscapes. Ongoing plasma and tissue analyses will further clarify the energetic and cellular mechanisms underlying thermal and oxidative stress resilience in coastal fishes. Full article

In this study, hexagonal titanium dioxide nanotubes (hTNTs) fabricated by sonoelectrochemical anodization were thermally modified in air to investigate the influence of annealing temperature and heating/cooling rate on phase evolution, structural stability and electrochemical behavior. The samples were annealed at 450 °C, 550 °C, and 650 °C for 2 h using heating/cooling rates of 6 °C/min, 10 °C/min, and 20 °C/min. The hexagonal nanotubular morphology remained preserved after thermal treatment. However, increasing annealing temperature and heating/cooling rate promoted crack formation due to the thermally induced stress relaxation and phase transformation. The anatase content increased with increasing heating/cooling rate, indicating kinetically limited anatase-to-rutile transformation, whereas annealing at 650 °C promoted partial rutile formation. Electrochemical studies demonstrated that annealing temperature and heating/cooling rate affected the electrochemical behavior of hTNTs through different mechanisms. Increasing annealing temperature promoted structural ordering and partial anatase-to-rutile transformation, leading to reduced current response and enhanced electrochemical stability. In contrast, heating/cooling rate significantly affected impedance behavior and diffusion-related processes, indicating changes in charge transfer kinetics and ion transport within the nanotubular oxide layer. The results demonstrate that thermal treatment kinetics play an important role in controlling the phase composition and electrochemical behavior of hTNTs, providing insight into the thermal optimization of hexagonal TiO₂ nanotubes for advanced functional applications. Full article

Non-thermal millimeter-wave (MMW) irradiation represents a promising non-invasive strategy for cancer therapy, yet its effects in physiologically relevant 3D systems remain poorly defined. Here, we evaluated the biological impact of MMW exposure in 3D non-small-cell lung cancer (NSCLC) spheroids (NCI-H1299, A549) and normal WI-38 fibroblasts under active cooling to suppress bulk heating. We demonstrate that cellular responses are governed primarily by power density (PD), irradiation geometry, and genotype-dependent susceptibility. High-PD pyramidal horn (PH) irradiation (~4.9 mW/cm²) induced rapid apoptosis, metabolic collapse, and near-complete loss of clonogenic survival, whereas lower-PD waveguide (WG) irradiation (~0.6 mW/cm²) produced depth-limited, cumulative cytotoxicity. Surviving cancer cells exhibited robust senescence-associated growth arrest, particularly in p53-deficient NCI-H1299 cells, indicating a dual apoptotic–senescent anti-proliferative response. In contrast, WI-38 fibroblasts showed minimal apoptosis and only transient stress-associated senescence, confirming selective tumor vulnerability. Mechanistic modeling suggests that MMW energy couples to glycan-rich membrane domains, generating localized electromagnetic hotspots that trigger calcium influx, mitochondrial dysfunction, and depth-dependent apoptosis. These findings establish a mechanistic basis for selective, non-thermal MMW-induced cytotoxicity in 3D NSCLC models and support further preclinical development of MMW-based therapeutic strategies. Full article

This study evaluates the ecological impacts of seawater desalination discharge on coastal marine ecosystems through a sequential analytical framework linking systematic literature synthesis, field-monitoring evidence, spatial analysis, and predictive ecological modeling. The novelty of the study lies in combining multi-regional evidence from Mediterranean coastal zones, Persian Gulf waters, and Pacific coastal environments with threshold-based ecological risk assessment, thereby linking discharge-related environmental stressors with biological responses and ecosystem-function alterations. The systematic review first retained 750 studies published between 2004 and 2024 for qualitative synthesis. On this basis, 59 high-quality references with sufficient numerical information were selected for the main quantitative meta-analysis, while field-monitoring data were used to support the interpretation of distance-based discharge gradients. Spatial interpolation and hierarchical modeling were then applied to evaluate exposure–response patterns and ecological threshold behavior. The results showed that desalination facilities generated measurable ecological impacts mainly within 50–200 m of discharge points, with a critical transition distance of approximately 127 m where hypersaline conditions, typically 1.5–2.0 times ambient seawater levels, were associated with marked changes in marine community structure. Benthic assemblages showed taxon-specific responses, with mollusks and echinoderms exhibiting greater sensitivity than polychaetes and small crustaceans. Marine vegetation declined strongly under combined salinity, thermal, and chemical stress, while phosphonate-based antiscalants accumulated in filter-feeding organisms and produced bioaccumulation factors up to 42.1 times ambient levels. Ecosystem-function indicators, including microbial community composition and sediment organic matter processing, remained altered up to 300 m from discharge points, indicating that functional impacts may extend beyond the primary hypersaline plume. The predictive modeling framework further demonstrated that ecological risk decreased nonlinearly with distance and varied according to discharge intensity, local hydrodynamics, and biological sensitivity. These findings indicate that conventional uniform buffer-based assessment may underestimate the ecological footprint of desalination discharge. Sustainable desalination management should therefore adopt site-specific monitoring, species-sensitive protection thresholds, improved brine-management technologies, and adaptive mitigation strategies based on real-time environmental feedback. Full article

Early-age temperature rise in mass concrete can generate substantial thermal gradients and increase the risk of cracking. In this study, a temperature-rise suppression concrete was developed by partially replacing conventional coarse aggregate with steel-encapsulated superabsorbent polymer (SAP)–water phase-change aggregates. Semi-adiabatic temperature-rise tests were conducted to characterize the early-age thermal response, and the corresponding adiabatic temperature-rise histories were reconstructed using a heat-loss compensation method. The results showed that the incorporation of steel-encapsulated SAP–water aggregates reduced the temperature rise and delayed the thermal peak under semi-adiabatic conditions. For SAP-15, the peak core temperature in the validated finite element simulation decreased from 51 °C to 44 °C, while the maximum adiabatic temperature rise decreased to 40.5 °C. Engineering-scale simulation of a bridge pile-cap foundation further showed reductions in internal peak temperature, temperature difference, and thermal stress. These findings demonstrate that steel-encapsulated SAP–water phase-change aggregates provide an effective material-based strategy for moderating early-age thermal accumulation and mitigating thermal cracking risk in mass concrete. Full article

Introduction: Temperature is a key environmental factor influencing the physiological and biochemical processes of aquatic organisms, including xenobiotic metabolism. Understanding how temperature modulates the toxicological effects of pollutants such as polycyclic aromatic hydrocarbons (PAHs) is crucial in the context of climate change. Among these compounds, benzo[a]pyrene (BaP) and benzo[a]anthracene (BaA) are priority pollutants in aquatic environments, resulting from incomplete combustion. Their relevance is attributed to persistence and metabolic bioactivation potential. Fish primary hepatocyte cultures represent a relevant in vitro model for studying combined effects of thermal stress and chemical exposures, while supporting the 3Rs principles (Replacement, Reduction, and Refinement). Objective: This study aims to assess temperature-dependent effects of BaP and BaA, and their mixtures in brown trout hepatocytes using cytochrome P450 1A (CYP1A) immunohistochemistry as an indicator of xenobiotic metabolism. Methodology: Primary hepatocytes were isolated using a two-step collagenase perfusion method and cultured in 24-well plates at 18 °C and 22 °C. Cells were exposed for 72 h to supplemented L-15 medium (control) or to 0.1% dimethyl sulfoxide in supplemented L-15 medium (solvent control), as well as to single exposures of 1 and 10 µM of BaP and BaA and to equimolar mixtures of both compounds (1 and 10 µM). Viability was assessed using the lactate dehydrogenase (LDH) assay. CYP1A immunostaining was quantified based on cytoplasmic staining intensity relative to background area. Results: No significant effects on cell viability were observed under any condition. Temperature significantly reduced CYP1A expression in single exposures at 22 °C compared to 18 °C. BaP induced a significant dose-dependent increase, while BaA differed from controls only at 10 µM. In mixtures, only treatment- and dose-dependent effects were observed, with no temperature influence detected. Conclusions: Overall, the data highlight temperature as a key modulator of biochemical responses to PAHs, with single and mixed exposures eliciting distinct effects and suggesting potential synergism in mixtures. Full article

Metal-organic framework (MOF)-based materials have become promising platforms for tumor hyperthermia by integrating energy conversion, tumor microenvironment regulation, and multimodal therapy within programmable porous structures. This review summarizes recent advances in intrinsic MOFs, MOF composites, and MOF-derived materials for photothermal therapy, microwave hyperthermia, and magnetic hyperthermia. The reviewed studies show that high-valence metal MOFs mainly provide stable and modifiable frameworks, whereas transition-metal, magnetic, and multimetallic MOFs contribute to redox regulation, ROS generation, magnetic response, and microwave energy dissipation. Beyond localized heat generation, MOF-based platforms enhance therapeutic efficacy by combining hyperthermia with chemotherapy, chemodynamic therapy, metabolic intervention, immunotherapy, and imaging guidance. These integrated strategies help overcome incomplete ablation, thermotolerance, oxidative stress resistance, and tumor recurrence. However, clinical translation is still limited by insufficient standardization, uncertain degradation behavior, metal-ion safety, and inadequate thermal dose control. Future development should emphasize mechanism-oriented design, controllable composition, long-term biosafety, and image-guided thermal regulation to advance MOF-based hyperthermia toward precise and clinically relevant cancer therapy. Full article

Freezing stress is one of the major abiotic factors limiting plant growth and productivity. This study evaluates the effects of diatomite (DTM) as a natural silicon-rich amendment on growth performance, physiological responses, and cold stress tolerance in barley (Hordeum vulgare L.). Seed priming and substrate application of DTM at different concentrations (5–20%) were used to assess morphological, biochemical, and ultrastructural changes under normal and low-temperature conditions. Results showed that DTM significantly enhanced root growth and biomass accumulation, with the most pronounced effect at 10% concentration. Treated plants exhibited improved survival under freezing stress, along with better preservation of leaf cellular structure and photosynthetic pigments. Biochemical analyses revealed reduced proline accumulation and decreased activity of key antioxidant enzymes, indicating alleviation of oxidative stress and improved redox balance. Electron microscopy confirmed the integration of diatomite particles into seed and tissue structures, providing physical reinforcement and thermal protection. Overall, diatomite acts as a multifunctional, environmentally safe soil amendment that enhances plant growth and improves tolerance to cold stress through combined physical and physiological mechanisms. Full article

Copper (Cu) thin films and meshes on polyethylene terephthalate (PET) substrates are promising flexible transparent conductive electrodes (TCEs), yet their practical use is limited by insufficient interfacial adhesion and poor oxidative stability on inert polymer substrates. This work addresses these issues via a synergistic strategy of interfacial layer engineering and maskless laser lithography-based aperiodic mesh patterning, systematically comparing ceramic (Al₂O₃) and metallic (NiCr) interfacial layers for PET-supported Cu films and fabricating Linear/Sinusoidal aperiodic Cu meshes with tailored performance. Magnetron sputtering shows that Ar plasma-activated NiCr interfacial layers form a gradient-alloyed interface with Cu via interdiffusion, achieving 5B-level adhesion, mitigating bending-induced stress concentration, and enhancing damp-heat resistance (85 °C/85% RH) by suppressing oxidation—outperforming brittle Al₂O₃ layers. Patterning the optimized Cu/NiCr/PET structure into micrometer-scale meshes yields a Linear design with superior optoelectronic performance (~10.8 Ω/sq sheet resistance, >87% transmittance at 550 nm) and a Sinusoidal design with enhanced bending robustness via stress delocalization. Microstructural and elemental analyses clarify the NiCr layer’s interfacial toughening and anti-oxidation mechanisms. Practical validation in flexible transparent heaters demonstrates rapid thermal response and >20 h continuous operational stability. This study provides a scalable design strategy for high-performance PET-supported Cu meshes, offering insights for interface and structural optimization of flexible metallic TCEs for next-generation optoelectronics. Full article

Coral reefs support marine biodiversity, fisheries, tourism, and coastal protection, but they are increasingly threatened by environmental stress and bleaching. Satellite-based reef monitoring has mainly relied on thermal metrics, especially Degree Heating Weeks (DHW), to represent bleaching risk. However, thermal exposure alone may not fully describe reef stress in optically complex coastal waters, where light availability, water clarity, and water-quality conditions can modify coral response. This limitation is important in Indonesia, where reefs span diverse coastal environments and many bleaching observations occur under relatively low DHW. In this study, we develop the Coral Reef Environmental Stress Index (CRESI), implemented as CRESI-Mamba, to estimate coral reef stress in Indonesia as a continuous and interpretable satellite-based stress index. CRESI-Mamba uses 26-week sequences of thermal variables from NOAA Coral Reef Watch and ocean-color variables from NASA Visible Infrared Imaging Radiometer Suite (VIIRS). The model decomposes the inferred stress into thermal, optical, and water-quality pathways, and maps the resulting stress index to bleaching probability for event-based evaluation. CRESI-Mamba was trained and evaluated using 8424 reef observations from eight Indonesian regions. In Leave-One-Region-Out cross-validation (LORO-CV), the model achieved a mean area under the receiver operating characteristic curve (AUC) of $0.795\pm 0.087$. In grouped 5-fold cross-validation, it achieved an AUC of $0.802\pm 0.024$, exceeding the DHW-only baseline ($0.627\pm 0.021$) and performing comparably to stronger thermal-only models, while providing a pathway-decomposed stress index. The estimated stress index separated bleached and not-bleached observations, with paired stress differences of $0.299\pm 0.098$ in LORO-CV and $0.281\pm 0.032$ in grouped 5-fold CV. Pathway analysis showed that the dominant stress pathway differed among regions, with optical stress dominant in several low-DHW bleaching cases. These results show that reef stress in Indonesia is better represented as a multi-pathway environmental condition than as thermal exposure alone. CRESI-Mamba provides a framework for interpreting satellite environmental histories as reef stress, while retaining bleaching probability as an evaluation output. Full article

Introduction: Coastal fishes can adapt to water warming and hypoxia; however, acute tolerance does not necessarily predict longer-term performance and survival. This may be especially important in sedentary, site-faithful species with limited escape to escape increasingly unfavorable habitats. We assessed the climate-related stress responses of the Lusitanian toadfish, Halobatrachus didactylus, a benthic estuarine fish from the Northeast Atlantic, to water warming and hypoxia. Objectives: We aimed to determine the aerobic energy budget, thermal limits (CTmax), and critical oxygen tension (Pcrit), as well as blood indicators of metabolism, altered physiology and systemic stress, as proxies for whole-organism homeostatic state, thereby informing future ecophysiological assessments and bioindicator development in a context of environmental change. Methodology: We determined standard, routine, and maximum metabolic rates; aerobic scope; and critical thermal maximum (CTmax) and critical oxygen (Pcrit) thresholds on a set of 134 individuals ranging from 12 to 160 g in weight. On a different set of individuals (n = 48; 76.3 ± 2.6 g; 16.1 ± 0.18 cm), we simulated 30 days of seasonal scenarios combining low and high temperature with normoxia or hypoxia, followed by integrated metabolic, hematological, biochemical, and multivariate analyses. Results: Acute trials showed high short-term resilience: H. didactylus had an exceptionally low standard metabolic rate and routine metabolic rate, high CTmax (34.82 ± 0.66 °C), and strong hypoxia tolerance (Pcrit 0.59–1.97 mg O₂ L⁻¹), although smaller individuals were more sensitive. After 30 days, however, warming more than doubled standard and routine metabolic rates, while warm hypoxia reduced metabolic output relative to warm normoxia, consistent with metabolic depression under compounded stressors. This treatment also showed shifts in glucose, liver mass, red blood cell count, and hematocrit, identifying warm, oxygen-poor water as the most physiologically costly scenario for this species. Conclusions: Together, these results show that high acute tolerance does not guarantee resilience to climate change. In sedentary fishes, survival may depend less on surviving extremes than on maintaining energetic balance, oxygen transport capacity, and physiological homeostasis in increasingly warm, oxygen-poor coastal habitats. Full article

Allicin, the most critical bioactive compound of garlic (Allium sativum L.), is of significant industrial importance when extracted at high purity while preserving its structural integrity. In this study, the combined use of supercritical-CO₂ (SC-CO₂) extraction and molecular distillation (MD) techniques was investigated to obtain garlic extracts with high allicin content from Gaziantep (Araban) garlic. The SC-CO₂ extraction process was optimized using Response Surface Methodology (RSM) within a range of 150–300 bar pressure, 50–80% co-solvent concentration and 0.5–3.0 mL/min solvent flow rate. The obtained extracts were characterized by LC-ESI-DAD-MS/MS, and their biological activities were evaluated using a comprehensive in vitro digestion model. Allicin in vitro digestion was performed using models simulating gastrointestinal conditions of young adults (<65 years) and older adults (>65 years), and its bioactive properties were comparatively evaluated. In the antimicrobial analysis, for SC-CO₂, a strong activity was demonstrated against Staphylococcus aureus and Escherichia coli in the oral phase of the in vitro digestion model, with inhibition zones of 36.33 mm and 26.50 mm in young samples and 34.67 mm and 25.83 mm in older samples, respectively. Owing to the immediate nucleophilic attack triggered by the subsequent alkaline pH shift and pancreatic enzymatic stress, free allicin underwent total structural degradation, falling below detectable limits within the intestinal chyme. In terms of purification performance, allicin content increased from 45.77% after SC-CO₂ extraction to 67.10% after molecular distillation. Crucially, due to the immediate nucleophilic attack driven by the subsequent alkaline pH shift and pancreatic enzymatic stress, free allicin underwent complete structural degradation and was rendered strictly undetectable within the intestinal chyme. This approach provides a sustainable and environmentally friendly purification strategy that effectively limits the thermal degradation of allicin. The results present a practical framework for the scalable production of allicin-rich nutraceutical intermediates and functional food ingredients. Full article

This paper develops a bi-level optimization framework for community energy systems to improve grid stability and strengthen resilience against supply–demand mismatches, with potential applicability to weather-driven operational stress. By incorporating demand-side response resources, with particular emphasis on the thermal storage potential of buildings, the proposed framework enhances the operational security and regulation capability of the system. At the upper level, energy operators determine dynamic electricity pricing strategies aimed at not only maximizing economic returns but also shaping load profiles toward smoother and more stable operation. At the lower level, a building thermal dynamic model is established, and the schedulable characteristics of flexible appliances, including electric water heaters, dishwashers, and washing machines, are exploited to reduce user-side energy costs while supporting peak load mitigation. Through iterative coordination between the two levels, the proposed method enables effective joint optimization of supply and demand. Simulation results indicate that the framework increases operator revenues through differentiated pricing and, at the same time, substantially lowers users’ electricity expenditures. In addition, by aggregating distributed flexible resources as a virtual buffering capacity, the proposed strategy helps reconcile the interests of both operators and users and further improves the resilience of the local power community energy system. Full article

This study investigates the nonlinear dynamic response and chaos evolution of a shape memory alloy hybrid composite (SMAHC) actuator beam under coupled thermal, harmonic, and aerodynamic excitations. A reduced-order nonlinear dynamic model was developed by combining Euler–Bernoulli beam theory, von Karman geometric nonlinearity, the Brinson SMA constitutive relation, and first-order piston-theory aerodynamics. The governing equations were derived from Hamilton’s principle, discretized by the weighted residual method, and solved using the Newmark-beta algorithm. Chaotic evolution was quantified using a largest Lyapunov exponent-based chaos intensity indicator rather than the exact Kolmogorov–Sinai entropy. The reduced-order model was compared with ABAQUS finite element simulations under representative coupled aerodynamic and harmonic loading. The MATLAB prediction and ABAQUS response gave a dominant frequency of approximately 9.50 Hz, close to the prescribed excitation frequency of 9.55 Hz, with peak displacement amplitudes of approximately 0.0285 mm and 0.0324 mm, respectively. A supplementary ABAQUS modal-frequency separation check supported the use of the two-mode reduced-order model for the dominant low-frequency response, while also clarifying its limitation for high-dimensional chaotic modal interactions. The parametric results showed that an increasing excitation amplitude and aerodynamic load promoted frequency broadening and chaotic transitions. The Lyapunov-based indicator rose near γ = 65 under λ* = 100 and near λ* = 328 under γ = 30. Temperature-dependent SMA recovery stress further shifted the transition threshold by modifying the effective stiffness and internal restoring action of the beam. These results provide a reduced-order framework for interpreting nonlinear response transitions in SMAHC actuator beams in thermo-aeroelastic environments. Full article

Rapid urbanization reshapes urban land systems and intensifies surface thermal heterogeneity, yet nonlinear day–night land surface temperature (LST) responses to grey–green spatial organization and building morphology remain insufficiently understood, particularly in thermally stressed areas across the urban–rural gradient. Using Xi’an, China, as a case study, this study develops a priority-area-based land–climate interaction framework. Priority areas were defined as grid cells where elevated LST coincided with relatively strong local explanatory relationships between LST and land-cover or morphological variables. Multiscale geographically weighted regression (MGWR), gradient boosting decision trees (GBDTs), SHAP-based interpretation, and threshold sensitivity analysis were combined to identify dominant drivers, nonlinear response patterns, and interaction structures of daytime and nighttime LST. The results show pronounced day–night differentiation: daytime hotspots were concentrated in the built-up core, whereas nighttime hotspots extended toward the urban–rural fringe. Daytime LST was mainly associated with building coverage and grey-space organization, while nighttime LST was more strongly related to mean building height and the cooling contribution of green-space coverage. The analysis further identified localized empirical response ranges for built-up intensity, grey-space connectivity, building height, and green-space coverage within the priority areas. These findings clarify how land-cover configuration and building morphology jointly shape day–night surface thermal responses and provide context-specific evidence for land-use planning and targeted urban heat mitigation. Full article