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24 pages, 1420 KB  
Article
Distributed Photovoltaic–Storage Hierarchical Aggregation Method Based on Multi-Source Multi-Scale Data Fusion
by Shaobo Yang, Xuekai Hu, Lei Wang, Guanghui Sun, Min Shi, Zhengji Meng, Zifan Li, Zengze Tu and Jiapeng Li
Electronics 2026, 15(2), 464; https://doi.org/10.3390/electronics15020464 - 21 Jan 2026
Viewed by 45
Abstract
Accurate model aggregation is pivotal for the efficient dispatch and control of massive distributed photovoltaic (PV) and energy storage (ES) resources. However, the lack of unified standards across equipment manufacturers results in inconsistent data formats and resolutions. Furthermore, external disturbances like noise and [...] Read more.
Accurate model aggregation is pivotal for the efficient dispatch and control of massive distributed photovoltaic (PV) and energy storage (ES) resources. However, the lack of unified standards across equipment manufacturers results in inconsistent data formats and resolutions. Furthermore, external disturbances like noise and packet loss exacerbate the problem. The resulting data are massive, multi-source, and heterogeneous, which poses severe challenges to building effective aggregation models. To address these issues, this paper proposes a hierarchical aggregation method based on multi-source multi-scale data fusion. First, a Multi-source Multi-scale Decision Table (Ms-MsDT) model is constructed to establish a unified framework for the flexible storage and representation of heterogeneous PV-ES data. Subsequently, a two-stage fusion framework is developed, combining Information Gain (IG) for global coarse screening and Scale-based Trees (SbT) for local fine-grained selection. This approach achieves adaptive scale optimization, effectively balancing data volume reduction with high-fidelity feature preservation. Finally, a hierarchical aggregation mechanism is introduced, employing the Analytic Hierarchy Process (AHP) and a weight-guided improved K-Means algorithm to perform targeted clustering tailored to the specific control requirements of different voltage levels. Validation on an IEEE-33 node system demonstrates that the proposed method significantly improves data approximation precision and clustering compactness compared to conventional approaches. Full article
(This article belongs to the Section Industrial Electronics)
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17 pages, 759 KB  
Article
Unsupervised Detection of SOC Spoofing in OCPP 2.0.1 EV Charging Communication Protocol Using One-Class SVM
by Aisha B. Rahman, Md Sadman Siraj, Eirini Eleni Tsiropoulou, Georgios Fragkos, Ryan Sullivant, Yung Ryn Choe, Jhaell Jimenez, Junghwan Rhee and Kyu Hyung Lee
Future Internet 2026, 18(1), 60; https://doi.org/10.3390/fi18010060 - 21 Jan 2026
Viewed by 74
Abstract
The electric vehicles (EVs) market keeps growing globally; thus, it is critical to secure the EV charging communication protocols in order to guarantee reliable and fair charging operations among the customers. The Open Charge Point Protocol (OCPP) 2.0.1 supports the communication between the [...] Read more.
The electric vehicles (EVs) market keeps growing globally; thus, it is critical to secure the EV charging communication protocols in order to guarantee reliable and fair charging operations among the customers. The Open Charge Point Protocol (OCPP) 2.0.1 supports the communication between the Electric Vehicle Supply Equipment (EVSE) and Charging Station Management Systems (CSMSs); therefore, it becomes vulnerable to several types of attacks, which aim to jeopardize smart charging, billing, and energy management. Specifically, OCPP 2.0.1 allows the self-reporting of the State of Charge (SOC) values, which makes it vulnerable to spoofing-based cyberattacks, which target manipulating the scheduling priorities, distorting the load forecasts, and extending the charging sessions in an unfair manner. In this paper, we try to address this type of attack by providing a comprehensive analysis of the SOC spoofing attacks and introducing a novel unsupervised detection framework based on the One-Class Support Vector Machine (OCSVM) algorithm. Specifically, two types of attack scenarios are analyzed (i.e., priority manipulation and session extension) by deriving engineered features that capture the nonlinear relationships under normal charging behavior. Detailed simulation-based results are derived by utilizing the DESL-EPFL Level 3 EV charging dataset. Our results demonstrate high F1-score and recall in identifying spoofed SOC values and that the proposed OCSVM model demonstrates superior performance compared to alternative clustering and deep-learning based detectors. Full article
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24 pages, 7972 KB  
Article
YOLO-MCS: A Lightweight Loquat Object Detection Algorithm in Orchard Environments
by Wei Zhou, Leina Gao, Fuchun Sun and Yuechao Bian
Agriculture 2026, 16(2), 262; https://doi.org/10.3390/agriculture16020262 - 21 Jan 2026
Viewed by 75
Abstract
To address the challenges faced by loquat detection algorithms in orchard settings—including complex backgrounds, severe branch and leaf occlusion, and inaccurate identification of densely clustered fruits—which lead to high computational complexity, insufficient real-time performance, and limited recognition accuracy, this study proposed a lightweight [...] Read more.
To address the challenges faced by loquat detection algorithms in orchard settings—including complex backgrounds, severe branch and leaf occlusion, and inaccurate identification of densely clustered fruits—which lead to high computational complexity, insufficient real-time performance, and limited recognition accuracy, this study proposed a lightweight detection model based on the YOLO-MCS architecture. First, to address fruit occlusion by branches and leaves, the backbone network adopts the lightweight EfficientNet-b0 architecture. Leveraging its composite model scaling feature, this significantly reduces computational costs while balancing speed and accuracy. Second, to deal with inaccurate recognition of densely clustered fruits, the C2f module is enhanced. Spatial Channel Reconstruction Convolution (SCConv) optimizes and reconstructs the bottleneck structure of the C2f module, accelerating inference while improving the model’s multi-scale feature extraction capabilities. Finally, to overcome interference from complex natural backgrounds in loquat fruit detection, this study introduces the SimAm module during the initial detection phase. Its feature recalibration strategy enhances the model’s ability to focus on target regions. According to the experimental results, the improved YOLO-MCS model outperformed the original YOLOv8 model in terms of Precision (P) and mean Average Precision (mAP) by 1.3% and 2.2%, respectively. Additionally, the model reduced GFLOPs computation by 34.1% and Params by 43.3%. Furthermore, in tests under complex weather conditions and with interference factors such as leaf occlusion, branch occlusion, and fruit mutual occlusion, the YOLO-MCS model demonstrated significant robustness, achieving mAP of 89.9% in the loquat recognition task. The exceptional performance serves as a robust technical base on the development and research of intelligent systems for harvesting loquats. Full article
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22 pages, 5115 KB  
Article
Intelligent Detection Method of Defects in High-Rise Building Facades Using Infrared Thermography
by Daiming Liu, Yongqiang Jin, Yuan Yang, Zhenyang Xiao, Zeming Zhao, Changling Gao and Dingcheng Zhang
Sensors 2026, 26(2), 694; https://doi.org/10.3390/s26020694 - 20 Jan 2026
Viewed by 230
Abstract
High-rise building facades are prone to defects due to prolonged exposure to complex environments. Infrared detection, as a commonly employed method for facade defect inspection, often results in low accuracy owing to abundant interferences and blurred defect boundaries. In this work, an intelligent [...] Read more.
High-rise building facades are prone to defects due to prolonged exposure to complex environments. Infrared detection, as a commonly employed method for facade defect inspection, often results in low accuracy owing to abundant interferences and blurred defect boundaries. In this work, an intelligent defect detection method for high-rise building facades is proposed. In the first stage of the proposed method, a segmentation model based on DeepLabV3+ is proposed to remove interferences in infrared images using masks. The model incorporates a Post-Decoder Dual-Branch Boundary Refinement Module, which is subdivided into a boundary feature optimization branch and a boundary-guided attention branch. Sub-pixel-level contour refinement and boundary-adaptive weighting are hence achieved to mitigate edge blurring induced by thermal diffusion and to enhance the perception of slender cracks and cavity edges. A triple constraint mechanism is also introduced, combining cross-entropy, multi-scale Dice, and boundary-aware losses to address class imbalance and enhance segmentation performance for small targets. Furthermore, superpixel linear iterative clustering (SLIC) is utilized to enforce regional consistency, hence improving the smoothness and robustness of predictions. In the second stage of the proposed method, a defect detection model based on YOLOV11 is proposed to process masked infrared images for detecting hollow, seepage, cracks and detachment. This work validates the proposed method using 180 infrared images collected via unmanned aerial vehicles. The experimental results demonstrate that the proposed method achieves a detection precision of 89.7%, an mAP@0.5 of 87.9%, and a 57.8 mAP@50-95. surpassing other algorithms and confirming its effectiveness and superiority. Full article
(This article belongs to the Section Fault Diagnosis & Sensors)
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18 pages, 14158 KB  
Article
Vision-Based Perception and Execution Decision-Making for Fruit Picking Robots Using Generative AI Models
by Yunhe Zhou, Chunjiang Yu, Jiaming Zhang, Yuanhang Liu, Jiangming Kan, Xiangjun Zou, Kang Zhang, Hanyan Liang, Sheng Zhang and Fengyun Wu
Machines 2026, 14(1), 117; https://doi.org/10.3390/machines14010117 - 19 Jan 2026
Viewed by 129
Abstract
At present, fruit picking mainly relies on manual operation. Taking the litchi (litchi chinensis Sonn.)-picking robot as an example, visual perception is often affected by illumination variations, low recognition accuracy, complex maturity judgment, and occlusion, which lead to inaccurate fruit localization. This study [...] Read more.
At present, fruit picking mainly relies on manual operation. Taking the litchi (litchi chinensis Sonn.)-picking robot as an example, visual perception is often affected by illumination variations, low recognition accuracy, complex maturity judgment, and occlusion, which lead to inaccurate fruit localization. This study aims to establish an embodied perception mechanism based on “perception-reasoning-execution” to enhance the visual perception and decision-making capability of the robot in complex orchard environments. First, a Y-LitchiC instance segmentation method is proposed to achieve high-precision segmentation of litchi clusters. Second, a generative artificial intelligence model is introduced to intelligently assess fruit maturity and occlusion, providing auxiliary support for automatic picking. Based on the auxiliary judgments provided by the generative AI model, two types of dynamic harvesting decisions are formulated for subsequent operations. For unoccluded main fruit-bearing branches, a skeleton thinning algorithm is applied within the segmented region to extract the skeleton line, and the midpoint of the skeleton is used to perform the first type of localization and harvesting decision. In contrast, for main fruit-bearing branches occluded by leaves, threshold-based segmentation combined with maximum connected component extraction is employed to obtain the target region, followed by skeleton thinning, thereby completing the second type of dynamic picking decision. Experimental results show that the Y-LitchiC model improves the mean average precision (mAP) by 1.6% compared with the YOLOv11s-seg model, achieving higher accuracy in litchi cluster segmentation and recognition. The generative artificial intelligence model provides higher-level reasoning and decision-making capabilities for automatic picking. Overall, the proposed embodied perception mechanism and dynamic picking strategies effectively enhance the autonomous perception and decision-making of the picking robot in complex orchard environments, providing a reliable theoretical basis and technical support for accurate fruit localization and precision picking. Full article
(This article belongs to the Special Issue Control Engineering and Artificial Intelligence)
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20 pages, 3982 KB  
Article
AI-Driven Decimeter-Level Indoor Localization Using Single-Link Wi-Fi: Adaptive Clustering and Probabilistic Multipath Mitigation
by Li-Ping Tian, Chih-Min Yu, Li-Chun Wang and Zhizhang (David) Chen
Sensors 2026, 26(2), 642; https://doi.org/10.3390/s26020642 - 18 Jan 2026
Viewed by 147
Abstract
This paper presents an Artificial Intelligence (AI)-driven framework for high-precision indoor localization using single-link Wi-Fi channel state information (CSI), targeting real-time deployment in complex multipath environments. To overcome challenges such as signal distortion and environmental dynamics, the proposed system integrates adaptive and unsupervised [...] Read more.
This paper presents an Artificial Intelligence (AI)-driven framework for high-precision indoor localization using single-link Wi-Fi channel state information (CSI), targeting real-time deployment in complex multipath environments. To overcome challenges such as signal distortion and environmental dynamics, the proposed system integrates adaptive and unsupervised intelligence modules into the localization pipeline. A refined two-stage time-of-flight (TOF) estimation method is introduced, combining a minimum-norm algorithm with a probability-weighted refinement mechanism that improves ranging accuracy under non-line-of-sight (NLOS) conditions. Simultaneously, an adaptive parameter-tuned DBSCAN algorithm is applied to angle-of-arrival (AOA) sequences, enabling unsupervised spatio-temporal clustering for stable direction estimation without requiring prior labels or environmental calibration. These AI-enabled components allow the system to dynamically suppress multipath interference, eliminate positioning ambiguity, and maintain robustness across diverse indoor layouts. Comprehensive experiments conducted on the Widar2.0 dataset demonstrate that the proposed method achieves decimeter-level accuracy with an average localization error of 0.63 m, outperforming existing methods such as “Widar2.0” and “Dynamic-MUSIC” in both accuracy and efficiency. This intelligent and lightweight architecture is fully compatible with commodity Wi-Fi hardware and offers significant potential for real-time human tracking, smart building navigation, and other location-aware AI applications. Full article
(This article belongs to the Special Issue Sensors for Indoor Positioning)
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25 pages, 1857 KB  
Article
Exponentially Clustered Synchronization of a Stochastic Complex Network with Reaction–Diffusion Terms and Time Delays via a Pinning Boundary Control
by Binglong Lu and Mei Liu
Mathematics 2026, 14(2), 309; https://doi.org/10.3390/math14020309 - 15 Jan 2026
Viewed by 108
Abstract
A pinning boundary control strategy that can achieve the exponentially clustered synchronization of a specific class of complex networks is developed. Firstly, the studied model captures the essential features of networks, including spatial dependence, stochastic switching, noise perturbation, and time delays. Secondly, the [...] Read more.
A pinning boundary control strategy that can achieve the exponentially clustered synchronization of a specific class of complex networks is developed. Firstly, the studied model captures the essential features of networks, including spatial dependence, stochastic switching, noise perturbation, and time delays. Secondly, the proposed control algorithm can save the implementation cost and overcome environmental constraint by acting on the boundary of a few nodes. Thirdly, an average state related to the directed topology of the nodes in the same cluster is calculated as the target network. Finally, nonlinear simulations show that the proposed controller can solve the cluster synchronization of a directed coupled reaction–diffusion neural network with Markovian switching, stochastic noise and time delay. Full article
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26 pages, 4043 KB  
Article
A Machine Learning Approach for the Completion, Augmentation and Interpretation of a Survey on Household Food Waste Management
by Athanasia Barka-Papadimitriou, Vassilis Lyberatos, Eleni Desiotou, Kostas Efthimiou and Gerasimos Lyberatos
Processes 2026, 14(2), 302; https://doi.org/10.3390/pr14020302 - 15 Jan 2026
Viewed by 276
Abstract
Households are the major contributor to food waste generation in the European Union according to the recently published data from Eurostat. Promoting food systems sustainability and aspiring to achieve the United Nations SDG 12.3 requires a better insight to the underlying drivers of [...] Read more.
Households are the major contributor to food waste generation in the European Union according to the recently published data from Eurostat. Promoting food systems sustainability and aspiring to achieve the United Nations SDG 12.3 requires a better insight to the underlying drivers of the household food waste occurrence. The present study presents the combination of a well-established method of acquiring information, the questionnaire surveys, with a state-of-the-art technology for data imputation and interpretation using machine learning (ML). The Food Loss and Waste Prevention Unit (FLWPU) of the municipality of Halandri employed two surveys within the framework of the European funded projects Food Connections and FOODRUS. The first questionnaire was designed for rapid completion, to maximize response rates and minimize respondent burden, ensuring the collection of a consistent core dataset. A total of 154 replies were collected. The second questionnaire, associated with FOODRUS, was more detailed, enabling the participants to provide more in-depth information on their household food waste (HHFW) practices. In total, 43 responses were collected. ML algorithms were applied for data enhancement and data clustering. Specifically, ML and statistical techniques are applied for data imputations. An XGBoost algorithm was trained so as to capture complex relationships between variables. Behavioral intentions and effective strategies for reducing food waste at the community level are identified from the responses of both questionnaires, while a clustering of respondents in five groups emerged by using k-means, thus providing valuable insight into targeted HHFW prevention action plans. Full article
(This article belongs to the Special Issue 1st SUSTENS Meeting: Advances in Sustainable Engineering Systems)
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22 pages, 6609 KB  
Article
CAMS-AI: A Coarse-to-Fine Framework for Efficient Small Object Detection in High-Resolution Images
by Zhanqi Chen, Zhao Chen, Baohui Yang, Qian Guo, Haoran Wang and Xiangquan Zeng
Remote Sens. 2026, 18(2), 259; https://doi.org/10.3390/rs18020259 - 14 Jan 2026
Viewed by 159
Abstract
Automated livestock monitoring in wide-area grasslands is a critical component of smart agriculture development. Devices such as Unmanned Aerial Vehicles (UAVs), remote sensing, and high-mounted cameras provide unique monitoring perspectives for this purpose. The high-resolution images they capture cover vast grassland backgrounds, where [...] Read more.
Automated livestock monitoring in wide-area grasslands is a critical component of smart agriculture development. Devices such as Unmanned Aerial Vehicles (UAVs), remote sensing, and high-mounted cameras provide unique monitoring perspectives for this purpose. The high-resolution images they capture cover vast grassland backgrounds, where targets often appear as small, distant objects and are extremely unevenly distributed. Applying standard detectors directly to such images yields poor results and extremely high miss rates. To improve the detection accuracy of small targets in high-resolution images, methods represented by Slicing Aided Hyper Inference (SAHI) have been widely adopted. However, in specific scenarios, SAHI’s drawbacks are dramatically amplified. Its strategy of uniform global slicing divides each original image into a fixed number of sub-images, many of which may be pure background (negative samples) containing no targets. This results in a significant waste of computational resources and a precipitous drop in inference speed, falling far short of practical application requirements. To resolve this conflict between accuracy and efficiency, this paper proposes an efficient detection framework named CAMS-AI (Clustering and Adaptive Multi-level Slicing for Aided Inference). CAMS-AI adopts a “coarse-to-fine” intelligent focusing strategy: First, a Region Proposal Network (RPN) is used to rapidly locate all potential target areas. Next, a clustering algorithm is employed to generate precise Regions of Interest (ROIs), effectively focusing computational resources on target-dense areas. Finally, an innovative multi-level slicing strategy and a high-precision model are applied only to these high-quality ROIs for fine-grained detection. Experimental results demonstrate that the CAMS-AI framework achieves a mean Average Precision (mAP) comparable to SAHI while significantly increasing inference speed. Taking the RT-DETR detector as an example, while achieving 96% of the mAP50–95 accuracy level of the SAHI method, CAMS-AI’s end-to-end frames per second (FPS) is 10.3 times that of SAHI, showcasing its immense application potential in real-world, high-resolution monitoring scenarios. Full article
(This article belongs to the Section Remote Sensing Image Processing)
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16 pages, 579 KB  
Article
The Short-Tailed Golden Dog Fragmented Realm: α-Hull Unravels the Maned Wolf’s Hidden Population
by Luan de Jesus Matos de Brito
Wild 2026, 3(1), 4; https://doi.org/10.3390/wild3010004 - 13 Jan 2026
Viewed by 141
Abstract
Understanding the spatial structure of large mammals is critical for conservation planning, especially under increasing habitat fragmentation. This study applies an integrated spatial analysis combining the DBSCAN density-based clustering algorithm and the α-hull method to delineate non-convex geographic ranges of the maned wolf [...] Read more.
Understanding the spatial structure of large mammals is critical for conservation planning, especially under increasing habitat fragmentation. This study applies an integrated spatial analysis combining the DBSCAN density-based clustering algorithm and the α-hull method to delineate non-convex geographic ranges of the maned wolf (Chrysocyon brachyurus) across South America. Using 454 occurrence records filtered for ecological reliability, we identified 11 geographically isolated α-populations distributed across five countries and multiple biomes, including the Cerrado, Chaco, and Atlantic Forest. The sensitivity analysis of the α parameter demonstrated that values below 2 failed to generate viable polygons, while α = 2 provided the best balance between geometric detail and ecological plausibility. Our results reveal a highly fragmented distribution, with α-populations varying in area from 43,077 km2 to 566,154.7 km2 and separated by distances up to 994.755 km. Smaller and peripheral α-populations are likely more vulnerable to stochastic processes, genetic drift, and inbreeding, while larger clusters remain functionally isolated due to anthropogenic barriers. We propose the concept of ‘α-population’ as an operational unit to describe geographically and functionally isolated groups identified through combined spatial clustering and non-convex hull analysis. This approach offers a reproducible and biologically meaningful framework for refining range estimates, identifying conservation units, and guiding targeted management actions. Overall, integrating α-hulls with density-based clustering improves our understanding of the species’ fragmented spatial structure and supports evidence-based conservation strategies aimed at maintaining habitat connectivity and long-term viability of C. brachyurus populations. Full article
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33 pages, 3113 KB  
Article
Hierarchical Role-Based Multi-Agent Reinforcement Learning for UHF Radiation Source Localization with Heterogeneous UAV Swarms
by Yuanqiang Sun, Xueqing Zhang, Menglin Wang, Yangqiang Yang, Tao Xia, Xuan Zhu and Tonghe Cui
Drones 2026, 10(1), 54; https://doi.org/10.3390/drones10010054 - 12 Jan 2026
Viewed by 192
Abstract
With the continuous proliferation of radio frequency devices, electromagnetic environments in various regions are becoming increasingly complex. Effective monitoring of the electromagnetic environment and identification of interference sources have thus become critical tasks for maintaining order in the electromagnetic spectrum. In recent years, [...] Read more.
With the continuous proliferation of radio frequency devices, electromagnetic environments in various regions are becoming increasingly complex. Effective monitoring of the electromagnetic environment and identification of interference sources have thus become critical tasks for maintaining order in the electromagnetic spectrum. In recent years, rapid advances in UAV technology have spurred exploration of UAV-based electromagnetic spectrum monitoring as a novel approach. However, the limited payload capacity and endurance of UAVs constrain their monitoring capabilities. To address these challenges, we propose HMUDRL, a distributed heterogeneous multi-agent deep reinforcement learning algorithm. By leveraging cooperative operation between cluster-head UAVs (CH) and cluster-monitoring UAVs (CM) within a heterogeneous UAV swarm, HMUDRL enables high-precision detection and wide-area localization of UHF radiation source. Furthermore, we integrate a minimum-gap localization algorithm that exploits the spatial distribution of multiple CM to accurately pinpoint anomalous radiation sources. Simulation results validate the effectiveness of HMUDRL: in the later stages of training, the success rate of localizing target radiation sources converges to 96.1%, representing an average improvement of 1.8% over baseline algorithms; localization accuracy, measured by root mean square error (RMSE), is enhanced by approximately 87.3% compared to baselines; and communication overhead is reduced by more than 80% relative to homogeneous architectures. These results demonstrate that HMUDRL effectively addresses the challenges of data transmission control and sensing-localization performance faced by UAVs in UHF spectrum monitoring. Full article
(This article belongs to the Special Issue Cooperative Perception, Planning, and Control of Heterogeneous UAVs)
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63 pages, 23065 KB  
Article
Hierarchical Network Organization and Dynamic Perturbation Propagation in Autism Spectrum Disorder: An Integrative Machine Learning and Hypergraph Analysis Reveals Super-Hub Genes and Therapeutic Targets
by Larissa Margareta Batrancea, Ömer Akgüller, Mehmet Ali Balcı and Lucian Gaban
Biomedicines 2026, 14(1), 137; https://doi.org/10.3390/biomedicines14010137 - 9 Jan 2026
Viewed by 263
Abstract
Background/Objectives: Autism spectrum disorder (ASD) exhibits remarkable genetic heterogeneity involving hundreds of risk genes; however, the mechanism by which these genes organize within biological networks to contribute to disease pathogenesis remains incompletely understood. This study aims to elucidate these organizational principles and identify [...] Read more.
Background/Objectives: Autism spectrum disorder (ASD) exhibits remarkable genetic heterogeneity involving hundreds of risk genes; however, the mechanism by which these genes organize within biological networks to contribute to disease pathogenesis remains incompletely understood. This study aims to elucidate these organizational principles and identify critical network bottlenecks using a novel integrative computational framework. Methods: We analyzed 893 SFARI genes using a three-pronged computational approach: (1) a Machine Learning Dynamic Perturbation Propagation algorithm; (2) a hypergraph construction method explicitly modeling multi-gene complexes by integrating protein–protein interactions, co-expression modules, and curated pathways; and (3) Hypergraph Neural Network embeddings for gene clustering. Validation was performed using hub-independent features to address potential circularity, followed by a druggability assessment to prioritize therapeutic targets. Results: The hypergraph construction captured 3847 multi-way relationships, representing a 45% increase in biological relationships compared to pairwise networks. The perturbation algorithm achieved a 51% higher correlation with TADA genetic evidence than random walk methods. Analysis revealed a hierarchical organization where 179 hub genes exhibited a 3.22-fold increase in degree centrality and a 4.71-fold increase in perturbation scores relative to non-hub genes. Hypergraph Neural Network clustering identified five distinct gene clusters, including a “super-hub” cluster of 10 genes enriched in synaptic signaling (4.2-fold) and chromatin remodeling (3.9-fold). Validation confirmed that 8 of these 10 genes co-cluster even without topological information. Finally, we identified high-priority therapeutic targets, including ARID1A, POLR2A, and CACNB1. Conclusions: These findings establish hierarchical network organization principles in ASD, demonstrating that hub genes maintain substantially elevated perturbation states. The identification of critical network bottlenecks and pharmacologically tractable targets provides a foundation for understanding autism pathogenesis and developing precision medicine approaches. Full article
(This article belongs to the Special Issue Multidisciplinary Approaches to Neurodegenerative Disorders)
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16 pages, 1294 KB  
Article
Feature-Based Growth Curve Classification Enables Efficient Phage Discrimination
by Yuma Oka, Keidai Miyakawa, Moe Yamazaki and Yuki Maruyama
Viruses 2026, 18(1), 92; https://doi.org/10.3390/v18010092 - 9 Jan 2026
Viewed by 319
Abstract
Rapid isolation of therapeutic bacteriophages from environmental sources is essential for personalized phage therapy, particularly when appropriate phages are unavailable in existing banks. However, comprehensive characterization of all candidate phages is resource-intensive, especially when plaque morphologies are similar and fail to discriminate between [...] Read more.
Rapid isolation of therapeutic bacteriophages from environmental sources is essential for personalized phage therapy, particularly when appropriate phages are unavailable in existing banks. However, comprehensive characterization of all candidate phages is resource-intensive, especially when plaque morphologies are similar and fail to discriminate between distinct phages. Here, we present an upstream screening approach that utilizes co-culture growth curve analysis to rapidly triage phage isolates during the early isolation process. We extracted seven biologically meaningful features that capture lysis kinetics, lysis efficiency, and post-lysis dynamics from bacterial growth curves and applied unsupervised clustering algorithms for phage discrimination. Validation using T-phages at a multiplicity of infection of 0.01 demonstrated superior clustering performance (Adjusted Rand Index = 0.881 ± 0.057) compared to established metrics including the Virulence Index and Centroid Index. Application to phages isolated from sewage successfully identified all three genomically distinct species present (sampling score = 1.0), enabling targeted selection of representative phages for downstream characterization. This approach reduced candidates requiring detailed analysis by two-thirds (from 21 to 7 isolates) while maintaining complete species coverage, thereby providing an efficient and scalable screening tool that reduces workload for downstream analyses and accelerates discovery of novel therapeutic phages for clinical applications. Full article
(This article belongs to the Collection Phage Therapy)
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25 pages, 4983 KB  
Article
Online Synchronous Coordinated Assignment and Planning for Heterogeneous Fixed-Wing UAVs
by Xindi Wang, Jiansong Zhang, Zhenyu Ma, Chuanshuo Cao and Hao Liu
Aerospace 2026, 13(1), 69; https://doi.org/10.3390/aerospace13010069 - 8 Jan 2026
Viewed by 212
Abstract
This paper addresses the Multi-Target Reconnaissance (MTR) problem for heterogeneous Fixed-Wing Unmanned Aerial Vehicles (FW-UAVs), focusing on synchronized and time-optimal mission execution under stringent constraints. A two-stage coordinated assignment and planning framework is proposed. First, a time-balanced clustering algorithm is designed to minimize [...] Read more.
This paper addresses the Multi-Target Reconnaissance (MTR) problem for heterogeneous Fixed-Wing Unmanned Aerial Vehicles (FW-UAVs), focusing on synchronized and time-optimal mission execution under stringent constraints. A two-stage coordinated assignment and planning framework is proposed. First, a time-balanced clustering algorithm is designed to minimize the overall mission duration while balancing individual UAV workloads by jointly employing a target reallocation strategy and an improved Genetic Algorithm (GA). Subsequently, an online trajectory planning method based on differential flatness is developed, integrating a robust replanning and flight-time synchronization strategy to ensure coordinated execution. Simulation results unequivocally demonstrate that the proposed approach enhances time optimality and temporal coordination in complex scenarios. Full article
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15 pages, 1214 KB  
Article
Work Complexity and Musculoskeletal Symptoms in Healthcare Workers
by Elamara Marama de Araujo Vieira, Jonhatan Magno Norte da Silva, Wilza Karla dos Santos Leite and Gilvane de Lima Araújo
Healthcare 2026, 14(1), 135; https://doi.org/10.3390/healthcare14010135 - 5 Jan 2026
Viewed by 268
Abstract
Background/Objectives: To investigate whether healthcare workers present different characteristics of musculoskeletal symptoms depending on the level of complexity in which these professionals work in the Brazilian Unified Health System. Methods: Health professionals were recruited from 24 health institutions, using probabilistic stratified sampling. Data [...] Read more.
Background/Objectives: To investigate whether healthcare workers present different characteristics of musculoskeletal symptoms depending on the level of complexity in which these professionals work in the Brazilian Unified Health System. Methods: Health professionals were recruited from 24 health institutions, using probabilistic stratified sampling. Data were collected using the Nordic Musculoskeletal Questionnaire. We obtained the questionnaire scores through exploratory factor analysis. Based on the scores, individuals could be grouped into symptom configurations using a non-hierarchical clustering algorithm (K-means). Results: The created groups differed in symptom intensity and location but did not differ by level of work complexity, as defined by Brazil’s healthcare sector division. Therefore, regardless of the level of complexity at which professionals perform their activities in the Brazilian Unified Health System, the burden of musculoskeletal symptoms related to the factor under analysis is similar. We developed distinct symptom profiles for each group, accompanied by targeted occupational intervention recommendations. Conclusions: This study challenges conventional assumptions by demonstrating that musculoskeletal symptom burden remains consistent across varying levels of work complexity, while providing a practical framework for developing targeted interventions based on symptom profiles. Full article
(This article belongs to the Special Issue Job Stress, Physical and Mental Well-Being Among Workers)
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