Search Results (63)

Drought stress is a major abiotic factor limiting global crop productivity by disrupting cellular homeostasis, impairing photosynthesis, and restricting metabolic activity. Plant-associated microorganisms, including rhizobacteria, endophytes, and arbuscular mycorrhizal fungi, play key roles in enhancing drought resilience through molecular, biochemical, and physiological mechanisms. These beneficial microbes modulate phytohormone biosynthesis, enhance osmolyte accumulation, increase organic acid exudation, and activate ROS-scavenging antioxidant pathways. Microbe-mediated regulation of aquaporins, heat shock proteins, and root system architecture further improves water-use efficiency, hydraulic conductance, and stress acclimation. Advances in microbial genomics and systems biology have revealed the molecular drivers of plant–microbe synergism, enabling the development of tailored microbial consortia and next-generation bioinoculants. Complementarily, genetic and genome-guided modulation of drought-responsive regulatory hubs including transcription factors (DREB, NAC, MYB, bZIP), signal transducers (MAPKs, CDPKs), and protective proteins enhances adaptive plasticity under water deficit conditions. This review integrates current molecular insights into drought-induced perturbations in plants and highlights the convergence of microbial interventions and genome-guided strategies in reinforcing drought tolerance. Emphasizing mechanistic frameworks, scalable microbial technologies, and molecular breeding approaches, this work underscores their potential to improve crop resilience in increasingly water-limited environments. Full article

The development of bioactive food packaging is an important issue, given its potential to preserve food quality and safety without the use of synthetic preservatives. This study aimed to develop new polystyrene foam (PS) films with hydroxytyrosol (HOxTYR) and eugenol (EUG), alone or in combination, as bioactive molecules to preserve sliced sponge cake during long-term storage. The cake samples were analyzed periodically during storage at 15 °C in terms of quality attributes (pH, water activity, height, volume and weight loss, firmness, CIE Lab color, lipid peroxidation products, microbial spoilage, and overall acceptability) and shelf life. The active film containing the combination of 0.6% HOxTYR and 0.6% EUG showed the strongest antioxidant activity, which was attributed to a potential synergism between the compounds, resulting in lower lipid oxidation rates (TBARS). The combination of HOxTYR and EUG also offered the greatest reduction in bacterial load (62% for S. aureus and 58% for E. coli), suggesting a synergistic effect on microbial inhibition. Likewise, samples packaged in a modified atmosphere (MAP) with the active film containing the combination of HOxTYR and EUG showed the best performance, including a smoother texture and greater volume, more stable color, lower microbial counts, and greater overall acceptability, and, consequently, a longer shelf life of up to 70 days at room temperature. Furthermore, the results of this study could contribute to environmental protection by reducing food waste, and suggest that the developed active packaging technique represents a promising and innovative approach to the preservation of bakery products. Full article

Sodium butyrate has been documented to support gut function and help control pathogens in the gastrointestinal tract. However, the precise mechanisms of dietary sodium butyrate’s control over enteric pathogens in chickens remain unclear. Our study demonstrated that priming chicken bone marrow-derived macrophages (BMDMs) or the HD11 cell line with 1 mM sodium butyrate significantly enhanced their antimicrobial capacity against key bacterial pathogens (Escherichia coli, Salmonella Typhimurium, Pseudomonas aeruginosa, and Staphylococcus aureus) in gentamicin protection assays (p < 0.05; ≥1 log reduction in CFU/mL). This in vitro enhancement was associated with increased production of reactive oxygen species (ROS), as detected by DCFH-DA assays, showing approximately a 30% increase in HD11 cells and a 12% increase in BMDMs. Butyrate priming was observed to result in autophagy activation, potentially through mTOR pathway inhibition, evidenced by changes in related gene expression using RT-qPCR assay and a 2.5-fold increase in GFP-LC3B accumulation. Supporting this, pharmacological inhibition of ROS using the ROS scavenger N-acetyl-L-cystine (NAC) or autophagy with chloroquine reduced the butyrate-enhanced bacterial clearance. Furthermore, the mTOR inhibitor rapamycin synergized with butyrate priming, whereas the mTOR activator L-leucine counteracted enhanced antimicrobial activity. These findings offer crucial insights for improving host defence against bacterial infections and developing novel therapeutic strategies in chickens. Full article

The characterization of lithology within Quaternary aquifers holds significant geological importance for the protection, management, and utilization of groundwater resources, yet it continues to present considerable challenges. Indicator Kriging (IK) is a non-parametric, probability-based method of spatial interpolation. It considers the correlation and variability between data points, and its popularity stems from its alignment with geological experts’ principles. However, it still encounters issues in complex geological conditions. To address the limited capacity of conventional IK in reproducing geological variables within heterogeneous geological settings, this study develops an ordered IK method incorporating field variogram function parameters. This framework dynamically extends IK applications by integrating stratigraphic extension trends, requiring experts to formalize spatial variation trends into geological knowledge data, subsequently transformed into constraint parameters for interpolation. Estimation paths are determined via Euclidean distances between points-to-be-estimated and valid data, executing ordered IK following near-to-far and bottom-to-top principles. Results directly depict QLS formation spatial distributions or undergo expert modification for quantitative analysis, demonstrating superior integration of geological knowledge compared to empirical variogram fitting and partitioned IK estimation. The method reduces deviation from expert-interpreted spatial distributions while maintaining computational efficiency and multi-factor integration, with three case analyses confirming enhanced accuracy in lithology distribution reproduction and improved geostructural congruence in complex geological reconstruction. This approach revitalizes Kriging applications in complex geological research, synergizing domain cognition with computational efficacy to advance precision in geological characterization and support government decision-making. Full article

The contradiction between economic development and ecological protection has become a common challenge for territorial governance in developing countries around the world. However, extant studies have neglected the coupling and symbiotic relationship between humans and nature, resulting in significant functional conflicts, insufficient stability, and imbalances in ecological and economic benefits in the reconstruction of territorial spatial functional pattern (TSFP), making it difficult to achieve synergies between development and protection. The question that arises is how the TSFP can be reconstructed in order to achieve harmonious coexistence between humans and nature. This remains a challenging problem in the context of the synergizing development and protection of the TSFP. This study innovatively integrates set-theoretic principles and functional mappings with game-theoretic analysis to develop Territorial Spatial Functional Pattern Reconstruction (TSFPR) model designed to foster harmonious human–nature coexistence, and validates the model using geospatial data from Qionglai City, China. Empirical evidence demonstrates that, in comparison with conventional methods, TSFPR model significantly mitigates the territorial spatial functional conflicts (TSFCs), enhances stability and ecological and economic benefits, and achieves the expected harmonious coexistence between humans and nature. The analysis confirms that the territorial spatial functional conflict (TSFC) coordination index established in this study provides a reliable criterion for identifying superior territorial spatial functions (TSFs). The proposed TSFPR model is an expansion of the theory of spatial optimization modelling, and it provides a tool for reconstructing the TSFP for the harmonious coexistence between humans and nature. In summary, the utilization of the TSFPR model to reconstruct the TSFP for harmonious coexistence between humans and nature provides a novel solution for coordinating the development and protection of territorial space governance. Full article

Dendrobium denneanum Kerr, Dendrobium denneanum Kerr, an orchid in the food-medicine homology catalog, is traditionally used for stomach-nourishing, yin-tonifying, and immunity-enhancing. While its preventive effect on acute gastric ulcers is confirmed, variations among genuine producing areas remain underexplored. This study comparatively analyzed components of D. denneanum from 22 habitats and their polysaccharides’ (DDP) anti-inflammatory/antioxidant activities. Results showed habitat-dependent active components: total sugar (20–51.49%), crude polysaccharide yield (0.29–1.76%), and total phenol (~3%). In vitro, all extracts exhibited dose-dependent scavenging of DPPH (IC₅₀: 0.99–2.11 mg/mL), ABTS (0.61–1.62 mg/mL), and hydroxyl radicals (1.02–2.18 mg/mL), with Habitats 5 and 7 showing the strongest activity. GPC, ion chromatography, and FT-IR revealed DDP had a 5–11 kDa molecular weight, dominated by glucose (49.67–84.73%), plus mannose (8.29–12.25%) and galactose (0.96–16.41%), with shared hydroxyl (3400 cm⁻¹) and β-glycosidic bond (890 cm⁻¹) features. In ethanol-induced gastric ulcer rats, DDP exerted dose-dependent protection: low doses (100 mg/kg/d) reduced ulcer index, increased SOD/GSH-Px (1.5–1.8-fold), decreased MDA (30–35%), and elevated PGE₂; high doses (400 mg/kg/d) further inhibited serum TNF-α/IL-6 (25–40%) and improved histopathology. Conclusion: Despite habitat-dependent component variations, DDP maintains consistent structures. This study first confirms DDP protects gastric mucosa via antioxidant-anti-inflammatory synergism, supporting its development as a natural gastroprotectant. Future work may focus on standardized cultivation and clinical translation. Full article

Broad beans, natural sources of L-DOPA and bioactive phenolics show promise for Parkinson’s disease intervention. This study investigated broad bean extracts’ protective mechanisms against PD pathogenesis. Among screened varieties, QC25 extract exhibited optimal protection in MPP⁺-injured PC12 cells, improving viability, reducing LDH release, and mitigating cell cycle arrest. QC25 extract rescued mitochondrial dysfunction by suppressing ROS, restoring membrane potential, normalizing Ca²⁺ homeostasis, and recovering ATP synthesis. Metabolomics identified glycerophospholipid metabolism as the core protective pathway, mediating mitochondrial membrane stabilization. QC25 extract further activated PINK1/Parkin-mediated mitophagy, upregulating PINK1 and Parkin expression. Crucially, 6-gingerol—uniquely detected in QC25 extract—synergized with L-DOPA, enhancing cell viability and amplifying mitophagy through complementary mitochondrial repair mechanisms. These findings demonstrate QC25 broad bean variety exerts’ protective effects on PD model cells by regulating mitochondrial function and mitophagy, and its unique component 6-gingerol synergizes with L-DOPA to strengthen these effects. This study provides a theoretical basis for the development of QC25 as a functional food ingredient for neurological health maintenance. Full article

Nitric oxide (NO), the first gaseous molecule identified as a signaling mediator, plays a pivotal role in numerous physiological processes including cardiovascular regulation, immune response, and neurotransmission. Synthesized from L-arginine by nitric oxide synthase (NOS), NO exerts both protective and cytotoxic effects depending on its local concentration. At low levels, NO supports tumor growth by mitigating oxidative stress, while at high concentrations, it induces apoptosis through mechanisms such as p53 activation, cytochrome c release, and peroxynitrite formation. These dual properties position NO as a complex but promising agent in cancer therapy. Recent studies have highlighted the potential of NO in enhancing the efficacy of photodynamic therapy (PDT), where it synergizes with reactive oxygen species (ROS) to induce cytotoxic effects in tumor cells. Despite its promise, challenges such as rapid diffusion and limited tumor accumulation hinder NO’s therapeutic utility. This has spurred the development of NO donors and nanotechnology-based delivery systems to enable controlled, site-specific release. Moreover, NO has been shown to counteract multidrug resistance, improve tumor perfusion by dilating vasculature, and potentiate ROS-based therapies like PDT and radiotherapy. However, an emerging concern is NO’s role in promoting proliferation and migration of non-targeted “bystander” tumor cells following PDT-induced stress, primarily through iNOS upregulation. This feedback loop can contribute to tumor aggressiveness and metastasis, underscoring the need for a deeper understanding of NO’s molecular actions. While iNOS inhibitors show preclinical promise in various inflammatory and neoplastic conditions, no such agents have reached clinical approval, due to the complexity and context-dependent effects of NO. Future research should focus on refining NO delivery systems, developing selective iNOS inhibitors, and elucidating NO’s dual role in cancer biology to fully harness its therapeutic potential in PDT and beyond. Full article

This study, taking Anhui Province as a case study, systematically evaluated the spatiotemporal differentiation characteristics of six ecosystem services (biodiversity maintenance, water yield, carbon fixation, vegetation net primary productivity (NPP), soil retention, and crop production) from 2000 to 2020 through the integration of multi-stakeholder decision-making preferences and the Marxan model. Four conservation scenarios (ecological security priority, social benefit orientation, minimum cost constraint, and balance synergy) were established to explore the spatial optimization pathways of ecological protection zones under differentiated policy objectives. The findings indicated that: (1) The ecosystem services in Anhui Province exhibited a “low north and high south” spatial gradient, with significant synergies observed in natural ecosystem services in the southern Anhui mountainous areas, while the northern Anhui agricultural areas were subjected to significant trade-offs due to intensive development. (2) High service provision in the southern Anhui mountainous areas was maintained by topographic barriers and forest protection policies (significant NPP improvement zones accounted for 50.125%), whereas soil–water services degradation in the northern Anhui plains was caused by agricultural intensification and groundwater overexploitation (slight soil retention degradation covered 24.505%, and water yield degradation areas reached 29.766%). Urbanization demonstrated a double-edged sword effect—the expansion of the Hefei metropolitan area triggered suburban biodiversity degradation (significant degradation patches occupied 0.0758%), while ecological restoration projects promoted mountain NPP growth, highlighting the necessity of synergizing natural recovery and artificial interventions. (3) Multi-scenario planning revealed that the spatial congruence between the ecological security priority scenario and traditional ecological protection redlines reached 46.57%, whereas the social benefit scenario achieved only 12.13%, exposing the inadequate responsiveness of the current conservation framework to service demands in densely populated areas. This research validated the technical superiority of multi-objective systematic planning in reconciling ecological protection and development conflicts, providing scientific support for optimizing ecological security patterns in the Yangtze River Delta region. Full article

To address the protective demands of marine engineering equipment in complex corrosive environments, this study proposes an environmentally friendly composite coating based on carboxylated graphene oxide (CGO)-modified water-based epoxy organosilicon resin. By incorporating varying mass fractions (0.05–0.25%) of CGO into the resin matrix via mechanical blending, the microstructure, corrosion resistance, and long-term corrosion kinetics of the coatings were systematically investigated. The results demonstrate that the coating with 0.15 wt.% CGO (designated as KCG15) exhibited optimal comprehensive performance: its corrosion current density (I_corr = 4.37 × 10⁻⁸ A/cm²) was two orders of magnitude lower than that of the pure resin coating, while its low-frequency impedance modulus (∣Z∣_0.1Hz = 4.99 × 10⁶ Ω⋅cm²) is significantly enhanced, accompanied by improved surface compactness. The coating achieved a 97% inhibition rate against sulfate-reducing bacteria (SRB) through synergistic physical disruption and electrostatic repulsion mechanisms. Long-term corrosion kinetics analysis via 60-day seawater immersion identified three degradation phases—permeation (0–1 day), blockage (1–4 days), and failure (7–60 days)—with structural evolution from microcrack networks to foam-like blistering ultimately reducing by 97.8%. Furthermore, a 180-day atmospheric exposure test confirms the superior weatherability and adhesion of the KCG15 coating, with only minor discoloration observed due to its hydrophobic surface. This work provides theoretical and technical foundations for developing marine anti-corrosion coatings that synergize environmental sustainability with long-term protective performance. Full article

With the continuous development of the economy and technology, environmental problems in China have become increasingly prominent. Researching how financial technology and green finance impact environmental efficiency is crucial when it comes to finding methods to lower the environmental cost generated by economic activities. This study measured the development level of financial technology and green finance by using the text mining method and the entropy weight TOPSIS. Meanwhile, this study empirically analyzed the influence of financial technology and green finance on environmental efficiency and their mechanisms of action by using the fixed-effect model and the moderation effect model. The results show the following: (1) Financial technology and green finance directly promote environmental efficiency. (2) Financial technology can synergize with green finance to promote improvements in environmental efficiency. (3) Heterogeneity analysis shows that there is a “path dependence” effect in the development of financial technology and green finance. Fintech and green finance had a stronger synergistic effect in improving environmental efficiency in the eastern region with strong technological innovation transformation capabilities, higher physical capital investment, and a larger regional population density. Our research results lay a foundation for the Chinese government to formulate policies related to financial technology, green finance, and environmental protection. Full article

Traditional villages are vital repositories of China’s historical and cultural heritage. To enhance protection precision, this study develops a novel risk assessment framework integrating three dimensions: the natural environment, tangible heritage elements, and disaster prevention infrastructure. The framework mainly uses GIS spatial analysis and SPSS-based statistical modeling. It integrates traditional dwelling density as a key factor in vulnerability zoning by depicting assessment units with weighted vulnerability indicators. The study overlays kernel density maps of traditional buildings with natural hazard susceptibility data. This enables classification of villages and clusters into hierarchical disaster prevention tiers (core, key, and general zones). Core zones, characterized by high-density heritage structures and elevated flood risks, require structural reinforcement and ecological engineering, while key zones employ adaptive protection technologies. By incorporating traditional building density as a weighted vulnerability indicator, the framework enables hierarchical disaster zoning through spatial coupling of kernel density maps and flood susceptibility data. Taking the results of Lingshui Village as an example, an individual analysis was made, and the elements of the village were identified. Fourteen traditional villages in Mentougou District were graded and partitioned. Correlation examination of zoning findings and property damage, as well as an independent evaluation of categorization results and degree of calamity, demonstrated a correlation between the two. Therefore, empirical validation in Beijing’s Mentougou District demonstrates the efficacy of this approach. The methodology further establishes cross-village collaborative defense mechanisms under a “conservation–development–protection” paradigm, aligning administrative boundaries with spatial agglomeration patterns. The study establishes a hierarchical disaster prevention evaluation system and a regional technical pathway to bridge individual and cluster-level protection. Finally, by synergizing traditional dwelling conservation with ecological resilience, it explores bidirectional optimization between cultural heritage preservation and disaster prevention efficacy. Full article

Background: Hereditary hemoglobin disorders are the most common globally distributed monogenic red cell diseases. The rights of women with thalassemia or sickle cell disease (SCD) to motherhood need to be protected by creating a roadmap to guide her, and her family network, along all the phases of the event. In fact, pregnancy in these vulnerable patients requires special attention and guidelines from the counseling stage (giving information about the special requirement and risks posed by their pregnancy with respect to the general population) the pre-conception stage, the early and mid-late pregnancy stage, to labor and lactation. The biocomplexity of these diseases requires a multidisciplinary team synergizing with gynecologists and obstetricians. In addition, the presence of a multicultural scenario requires healthcare workers to overcome stereotypes and adopt appropriate anthropological tools that might help them integrate the different cultural models of disease and motherhood. Methods: The Management Committee of the Society for Thalassemia and Hemoglobinopathies (SITE) selected and brought together a multidisciplinary and multiprofessional group made up of experts in hemoglobinopathies and experts in anthropology, flanked along with by experts with methodological and organizational expertise in order to create recommendations based on the integration of available scientific evidence together with expert opinion. Results: The panelists critically analyzed the literature, combining in a single document practices developed over several years of managing young women with hemoglobinopathies in a sensitive phase of their lives. Conclusions: This good practice document is the result of a collegial effort by Italian experts on hemoglobinopathies who are members of SITE. (SITE). Full article

Can government environmental policy harmonize environmental protection with economic output? We explore this issue from the perspective of forestry eco-efficiency, using China’s National Ecological Civilization Pilot Zone Policy (NECP), an environmental policy promulgated by the government of China, as the subject of this study. The study introduces forestry eco-efficiency as an indicator to assess the balance between economic development in the forestry sector and environmental conservation. The indicator, grounded in sustainable development theory, employs a super-efficiency SBM model that includes undesirable outputs to evaluate efficiency. Additionally, we empirically analyze the impact of NECP on forestry eco-efficiency by using the difference-in-difference (DID) model with provincial panel data from 2011 to 2020. Ultimately, we analyze the effects of spatial spillover by employing the spatial Durbin model (SDM). Our study yields the following conclusions. (1) In this paper, through hotspot clustering analysis, forestry eco-efficiency in each province is categorized into three categories: effective, semi-effective and ineffective. Our findings suggest that China’s average forestry eco-efficiency falls into the ineffective category, highlighting the need to optimize resource allocation within the sector. (2) NECP significantly enhances forestry eco-efficiency, with robust findings across various stability tests. Thus, implementing government environmental policies can have a multiplier effect on forestry, i.e., it can synergize its economic development with environmental protection. (3) In provinces with a strong ecological foundation, the NECP significantly enhances forestry eco-efficiency. However, in other provinces, the improvement is only moderate. Furthermore, while the NECP has a substantial positive impact in the eastern region, it has yet to show a discernible effect in other regions. (4) The positive impacts of NECP implementation on forestry eco-efficiency have spatial spillover effects due to demonstration effects and comparative advantages. Full article

Virgin olive oil (VOO) is a globally esteemed product renowned for its chemical composition, nutritional value, and health benefits. Consumers seeking natural, nutritious, and healthy foods increasingly favor VOO. The optimization of the extraction system ensures the production of high-quality VOO with abundant antioxidant compounds that naturally protect it from degradation. Proper storage is crucial in maintaining the quality of VOO, prompting the exploration of novel extraction and preservation techniques. Factors such as light, temperature, and oxygen greatly influence the degradation process, resulting in reduced levels of natural antioxidants like polyphenols. Undesirable by-products and non-aromatic compounds may be formed, making the oil unacceptable over time. On the basis of all this consideration, this study aimed to evaluate the synergic use of two different gases (CO₂ and argon) during the malaxation phase to limit radical development and delay lipid autoxidation. Additionally, unconventional preservation systems, namely argon headspace, shellac, and bottle in bag, were assessed over a period of 150 days. The results evidenced that the use of CO₂ and argon during the malaxation process resulted in an improvement in the oil quality compared to the one obtained with the traditional system. However, in traditional oils, the alternative packaging systems determined interesting outcomes as they were able to positively affect different parameters, while the packaging effect was more mitigated in the test oils. Full article