Search Results (244)

Objective: Sodium–glucose cotransporter 2 (SGLT2) inhibitors, particularly empagliflozin, have attracted considerable attention because of their cardiovascular benefits beyond glycemic control. However, the interaction between empagliflozin and exercise-induced physiological cardiac remodeling in healthy individuals remains insufficiently understood. This study investigated the effects of different swimming exercise protocols (anaerobic, aerobic, and endurance), administered alone or in combination with empagliflozin, on cardiac structure and electrophysiology. Methods: Thirty-six male Sprague–Dawley rats were randomly assigned to six groups (n = 6 per group): anaerobic (An), aerobic (Ae), endurance (En), and the corresponding exercise groups combined with empagliflozin (An + Empa, Ae + Empa, and En + Empa). Empagliflozin was administered by oral gavage at a dose of 15 mg/kg/day for 30 days. Transthoracic echocardiography, electrocardiography (ECG), and gastrocnemius electromyography were performed at baseline and at the end of the study to assess cardiac remodeling, heart rate, and neuromuscular function. The study was carried out over a 30-day intervention period following ethics committee approval on 24 July 2024. Results: No significant between-group differences were observed in echocardiographic parameters before the intervention. On day 30, significant differences were identified among the groups in interventricular septal thickness at end-diastole (IVSd) (p = 0.027), left ventricular internal diameter at end-diastole (LVIDd) (p = 0.009), and end-diastolic volume (EDV) (p = 0.014). Bonferroni-corrected post hoc analysis showed that the aerobic exercise plus empagliflozin group differed from several exercise-only groups, particularly in parameters related to ventricular size and filling volume, including LVIDd and EDV (p < 0.008). On day 30, electrocardiographic repolarization-related parameters, including QT, QTc, JT, and Tpeak–Tend intervals, also differed significantly among the groups (all p < 0.05). In post hoc analysis, the anaerobic exercise group showed significant differences in QT and JT intervals compared with the aerobic and endurance groups (p < 0.008). In the anaerobic protocol, empagliflozin was associated with a reduction in heart rate compared with the corresponding control group (p = 0.019). No significant between-group differences were observed in EMG findings. Conclusions: Different exercise protocols induce distinct patterns of adaptation in cardiac structure and electrophysiology in healthy rats. Empagliflozin (15 mg/kg/day) may modulate exercise-induced cardiac responses in a modality-dependent manner; the most pronounced echocardiographic effects were observed in the aerobic protocol, whereas the effect on heart rate was observed in the anaerobic protocol. These findings highlight the need for longer-term and mechanistic studies to further clarify the effects of SGLT2 inhibitors on physiological cardiac remodeling. Full article

Psilocybin, a psychedelic drug with reported anxiolytic and antidepressant potential, is rapidly metabolized to its active metabolite psilocin. However, a lack of adequate toxicity studies and tissue distribution studies currently restricts its development and application. This study combined behavioral assays in zebrafish with desorption electrospray ionization mass spectrometry imaging (DESI-MSI) to systematically evaluate the acute neurotoxicity of psilocybin and characterize the in vivo spatial distribution of its active metabolite, psilocin. The novel tank test was used to evaluate zebrafish following a 4 h exposure to psilocybin at three different doses (20, 40, and 80 μM; n = 6 per group). Statistical analysis of the data was performed using ANOVA. Behavioral analyses revealed that exposure to psilocybin induced pronounced neurobehavioral alterations, including hyperactivity and disrupted swimming patterns, as evidenced by significant increases in the number of zone transitions and shuttle frequency. We established a DESI-MSI-based method for quantitative mapping and visualization of psilocin in zebrafish tissues. Methodological validation indicated that a linear relationship between ion intensity, spotted amount (R² = 0.9947), and reproducibility (RSD < 15%) is suitable for quantitative analysis of psilocin in zebrafish tissues. Spatial distribution maps showed that following continuous exposure for 4 h, psilocin was widely distributed across multiple tissues, such as the eye, brain, heart, liver, and kidney, with marked accumulation in the brain and the periportal regions of the liver. Relative psilocin signal intensity revealed a dose-dependent increase in tissue drug levels. The dose-dependent increase in both behavioral hyperactivity and brain psilocin levels points to a consistent relationship, in line with a central site of action. Collectively, these findings demonstrate that DESI-MSI provides a visual and efficient strategy for studying drug distribution in biological tissues from exposed animals. The neurobehavioral toxicity phenotypes and distinct tissue distribution patterns of psilocin uncovered in this study offer critical insights into the biological effects and potential risks of this psychoactive substance. Full article

The transport and distribution of Scomber japonicus larvae significantly affect their habitat and population dynamics. Understanding these processes is crucial for developing effective fishing and conservation strategies. However, the interannual variability of the Kuroshio path introduces both complexity and uncertainty. This study implemented a coupled ocean–biophysical model to simulate and analyze the transport of S. japonicus larvae in the Pacific Ocean south of Japan across three Kuroshio path modes, including the offshore non-large-meander (ONLM), nearshore non-large-meander (NNLM), and typical large-meander (TLM) paths. Two transport scenarios, passive drift (PD) and active swimming (AS), were considered in the simulations. The simulated results presented a comprehensive analysis of the distribution, connectivity, and transport distances of S. japonicus larvae. These findings highlighted the significant influence of biological behavior on larval transport, notably reducing transport distances and shifting the distributions northward. This allowed larvae to actively migrate to areas with higher zooplankton aggregation. Larvae released from the western and nearshore spawning grounds around Southern Kyushu–Shikoku were mainly transported to the central nursery region between 132.5° E and 140° E, whereas larvae released from the eastern spawning grounds were mainly distributed in the eastern nursery region east of 140° E near the Kuroshio Extension. These patterns suggest that nursery areas 2 and 3 may warrant further attention in future spatial management assessments, particularly when considering larval transport under different Kuroshio path modes. This study provides valuable insights into the transport and distribution mechanisms of S. japonicus larvae, offering critical guidance for the conservation of fishery resources and the promotion of sustainable fishery management. Full article

The evolution of Osteichthyes began with a split into two major lineages: Sarcopterygii (lobe-finned fishes) and Actinopterygii (ray-finned fishes). In one lineage—sarcopterygians—some groups evolved robust internal bones and limb-like fins and ultimately gave rise to semi- and fully terrestrial tetrapods; the other lineage—actinopterygians—remained primarily aquatic and later radiated into the diverse teleosts. Repeated mass extinction events and ongoing genetic divergence allowed novel functions and new niches to be exploited, a pattern especially evident in recent analyses of teleost diversification. Lobe-finned fishes characteristically possess an endoskeleton fin architecture, whereas ray-finned fishes bear dermal fin rays built on a different structural plan. Primitive Osteichthyes also show an early origin of paired air-spaces (lungs), but many derived actinopterygians modified this ancestral condition into a dorsal swim bladder. Imagining a world without sarcopterygians or tetrapods highlights how teleosts might have convergently colonized many terrestrial-associated niches; although significant developmental and structural hurdles would have made such a transition challenging, this thought experiment underscores the cascading ecological consequences that the loss of a major clade can produce. Ecosystems thrive on diversity and adaptability, and episodes of environmental upheaval—such as the Silurian and Devonian extinctions—often catalyze rapid evolutionary change. Full article

To address the challenges posed by traditional tracking algorithms in adapting to complex underwater environments characterized by nonlinear motion, drastic morphological changes, mimicry camouflage, and frequent occlusions in wild Chinese giant salamanders, this study proposes a multi-object tracking and behavior analysis method based on the TransTrack-OC-SORT algorithm. The algorithm employs a dual-branch Transformer motion predictor to replace linear Kalman filtering, effectively capturing nonlinear motion patterns such as velocity changes and directional turns exhibited by salamanders. Simultaneously, it introduces the BIOU matching metric, which integrates center distance and aspect ratio penalties with overlap degree, thereby enhancing the robustness of associations in scenarios involving occlusion and mimicry camouflage. The results indicate that the multi-object tracking accuracy (MOTA) based on the TransTrack-OC-SORT algorithm reaches 80.9%, while the identity preservation metric (IDF1) achieves 83.7%. Quantitative behavioral analysis based on continuous trajectory data obtained from this algorithm revealed significant diurnal behavioral rhythms in the Chinese giant salamander. During the daytime, stationary behavior accounted for 95.5% of the total behavioral duration, while swimming for ventilation and foraging behaviors constituted only 4.1% and 0.4%, respectively. At night, the salamander’s behavioral patterns underwent fundamental changes; although stationary behavior remained dominant (approximately 72.6%), the proportions of swimming for ventilation and foraging behaviors significantly increased to 15.1% and 12.3% of the nocturnal period, respectively. These findings enhance our understanding of the salamander’s ecological habits from the perspectives of visual adaptation, energy allocation, and predation strategies. This study provides a reliable technical tool for non-invasive behavioral monitoring and rhythm research in endangered amphibians. Full article

Efficient segmental pitch control is critical for butterfly swimming propulsion and alignment, yet its role in youth performance remains unclear. This study quantified head, shoulder, and hip pitch kinematics using wearable inertial measurement units (IMUs) in 41 competitive swimmers (9–11 years). Participants performed two maximal 25-m butterfly trials and were classified into faster and slower groups. Pitch angle, velocity, frequency, time, and pitch deviation index were extracted. Between-group differences were assessed using independent t-tests, and associations with performance were examined using Pearson correlations. Faster swimmers exhibited smaller head pitch angles during the Breath phase (p < 0.001, d = −2.01), along with greater shoulder pitch velocities and frequencies (all p < 0.05, d = 0.67–1.07). They also demonstrated shorter pitch times and lower pitch deviation indices (all p < 0.05, d = 0.66–1.92), indicating more efficient and stable movement patterns. In contrast, hip kinematics showed fewer and less consistent differences between groups. Several head and shoulder variables during the Breath phase were moderately correlated with sprint time (r = 0.32–0.43, p < 0.05). These findings suggest that sprint butterfly performance in youth swimmers is primarily associated with more controlled and stable upper-body pitch motion, particularly during breathing. Full article

Introduction: Caffeine is a widely consumed adenosine receptor antagonist with well-documented effects on arousal and performance, but its time-resolved neurophysiological signature across stages of information processing remains fragmented across event-related potential (ERP) paradigms. Objectives: This systematic and mechanistic review aimed to (i) identify and catalog human ERP studies testing caffeine effects, (ii) synthesize findings by task domain and ERP component family, and (iii) evaluate moderators including dose, timing, abstinence/withdrawal control, sleep status, and habitual use. Methods: Following PRISMA 2020 and PRISMA-S, we searched multiple databases (PubMed/MEDLINE, Embase, APA PsycINFO, Web of Science Core Collection, Scopus, IEEE Xplore, and Cochrane Central Register of Controlled Trials) from inception to 28 November 2025 and conducted a structured narrative synthesis using SWiM (Synthesis Without Meta-analysis, no prespecified quantitative pooling). Risk of bias was assessed using RoB-2 (Risk of Bias 2, including crossover extension) and ROBINS-I (Risk Of Bias In Nonrandomized Studies of Interventions). Of 761 records, 63 controlled human studies met the inclusion criteria. The evidence most consistently supported stage- and context-dependent modulation. Within the P3 family, target-related P3b/P300 latency was frequently shortened, or fatigue-related slowing was prevented, often without parallel increases in amplitude. P300 amplitude findings were mixed and context-dependent: amplitude was often unchanged in rested or low-demand paradigms, but increased or was restored when caffeine counteracted fatigue, sleep loss, sustained attention demands, or high workload. Preparatory activity (CNV/slow negativity) showed selective effects, while early sensory components were comparatively stable in many paradigms; higher doses (approximately 200–400 mg) were associated with weaker early auditory sensory gating in some studies. Conclusions: Across heterogeneous paradigms, caffeine was associated with context-dependent ERP changes rather than a uniform amplification of ERP amplitudes. The most consistent pattern was shorter or preserved latency of late positive ERP components, particularly in tasks requiring stimulus evaluation or target detection. In some fatigue, sleep deprivation, sustained attention, or high-demand paradigms, caffeine was also associated with larger or restored P300/P3b amplitudes. These findings are compatible with state-dependent changes in attentional engagement or stimulus evaluation, but mechanistic interpretation remains limited by heterogeneity in task paradigms, ERP definitions, dosing, abstinence procedures, and participant caffeine use profiles. Methodological heterogeneity, small samples, inconsistent control of habitual use and withdrawal, and the predominance of healthy young adult samples limit generalizability, particularly to children, older adults, clinical populations, and long-term high-dose caffeine users. Full article

Professional competency frameworks are important for aligning educational outcomes with workforce needs. While multiple frameworks exist across sectors, the underlying research-informed design principles guiding their development remain fragmented. This systematic review synthesises methodological approaches and proposes research-informed design principles used in developing professional competency frameworks across diverse professions, identifying common patterns and informing future framework design. A systematic review was conducted following PRISMA 2020 and SWiM guidelines. Searches across major academic databases yielded 3656 records. After screening, 47 studies met inclusion criteria. Data extraction focused on methodological processes and development activities. Thematic analysis was used to generate a set of design principles: (1) Foundational Inquiry and Evidence Gathering; (2) Consensus-Building and Collaborative Validation; and (3) Framework Development and Iterative Refinement. The development of competency frameworks is inherently cyclical, interdisciplinary, and iterative, blending empirical inquiry with collaborative validation. The identified research-informed design principles offer a transferable blueprint applicable across sectors, from healthcare to education and other industries. Thus, it is strongly recommended that future studies use these initial research-informed design principles to inform competency development. The systematic review has been registered with Open Science Framework (OSF). Full article

Hydraulic engineering structures can threaten freshwater fish by entraining them into hazardous areas. Acoustic barriers have been proposed as a non-physical method to guide fish away from these zones. In this study, we investigated the behavioral responses of juvenile grass carp to different acoustic stimuli under semi-natural conditions using outdoor net cages. Four sound types were tested: a 1000 Hz pure tone and three broadband sounds, including Alligator sinensis hissing, pile-driving noise, and outboard motor noise. Behavioral responses were quantified using response frequency, total midline crossings, first-response time, maximum swimming speed, and average swimming speed. The results showed that Alligator sinensis hissing elicited the highest number of midline crossings, representing the strongest behavioral response among all tested sounds. In addition, both Alligator sinensis hissing and outboard motor noise induced significantly stronger avoidance responses than the pure tone or pile-driving noise, as indicated by higher response frequency and faster swimming speeds. Furthermore, manipulation of pulse repetition intervals in the most effective deterrent sounds generated a novel broadband sound, which altered fish distribution patterns and elicited avoidance behavior. These findings indicate that both sound type and temporal structure influence negative phonotaxis behavior in grass carp and provide experimental evidence for the optimization of acoustic barriers in fish management. Full article

The aim of this study was to understand the female swimmers’ annual performance progression and stability between 10 and 18 years across swimming distances and techniques. Data from female Portuguese Top-50 rankings in the short-course pool was extracted from an open access database (swimrankings.net). Performances were grouped by distances (50-, 100- and 200 m) and techniques (freestyle, backstroke, breaststroke and butterfly), totalizing 12 events as performance metrics. A total of 343 swimmers and 3087 performances distributed by nine consecutive competitive seasons were retrospectively assessed. The mean and normative stability were computed for tracking performance trends, while reporting the year-to-year percentage improvement. The differences across distances and techniques were tested with a linear mixed-effects model using intraclass correlation coefficient (ICC). The performance progression was characterized by marked improvements during the early ages (up to 13% yearly) and an emerging plateau around the 15–16 years. The stability patterns varied between events, with the backstroke technique (ICC = 0.13) demonstrating greater consistency of individual differences on developmental trajectories, whereas shorter races (i.e., 50 m; ICC = 0.15) tended to be more stable than 100 m or 200 m (ICC = 0.12). It can be concluded that female swimmers’ performance stabilizes at the 15–16 years of age. Despite reduced differences, the backstroke technique and short distances seem to show a slightly more stable trend in progressing from childhood to adulthood. Full article

Antimicrobial resistance in microorganisms associated with fermented foods is increasingly recognized, yet rapid methods to characterize antibiotic response dynamics remain limited. This study evaluates antibiotic susceptibility and physiological response patterns of kombucha-associated acetic acid bacteria and motile Escherichia coli using optical nanomotion detection (ONMD), a label-free technique that quantifies single-cell mechanical activity. Two cellulose-producing species (Komagataeibacter xylinus and K. rhaeticus), one non-cellulose-producing species (K. melaceti), and E. coli were exposed to ampicillin, ciprofloxacin, and chloramphenicol. Minimum inhibitory concentrations (MICs) were determined prior to time-resolved ONMD analysis. Susceptible strains exhibited progressive suppression of confined nanomotion consistent with MIC-defined susceptibility, whereas resistant profiles maintained sustained mechanical activity. Chloramphenicol initially induced persistent or increased nanomotion at 120 min; however, extending the observation to 180 min revealed delayed suppression in susceptible strains, demonstrating that bacteriostatic antibiotics require longer observation windows for accurate ONMD classification. In motile E. coli, ONMD revealed both intracellular nanomotion puncta and swimming trajectories, which were progressively attenuated following antibiotic exposure. These findings demonstrate that ONMD complements conventional susceptibility testing by resolving time-dependent suppression of both translational motility and intracellular nanomechanical activity at the single-cell level. Full article

Background: Psychoeducational interventions are increasingly implemented to promote psychological and physical health, yet evidence guiding personalized intervention selection remains limited. This systematic review and meta-analysis quantifies the effectiveness of psychoeducational interventions across five settings and identifies empirically derived moderator patterns to inform the selection of tailored interventions. Methods: Systematic searches of PubMed/MEDLINE, PsycINFO, Scopus, Web of Science, ERIC, the Cochrane Library, and Google Scholar were conducted to identify eligible studies published between January 2015 and December 2024. A two-tier analytical approach was employed: a random-effects meta-analysis of k = 53 studies reporting extractable effect-size data, and a direction-of-effect narrative synthesis of all 186 included studies (N = 50,328 verified from 124 studies reporting sample sizes), following SWiM guidelines. Results: The quantitative meta-analysis yielded a significant medium-to-large pooled effect (g = 0.66, 95% CI [0.50, 0.82], p < 0.001) with substantial heterogeneity (I² = 96.1%). Effects varied across settings: clinical/vulnerable populations showed the largest effect (g = 0.91), followed by university programs (g = 0.62), school-based (g = 0.60), mindfulness/positive psychology (g = 0.55), and community-based (g = 0.49). The broader narrative synthesis confirmed near-universal effectiveness: 131 studies (70.4%) reported significant positive effects, 51 (27.4%) reported mixed results, and none reported null effects—yielding 97.8% favorable outcomes across the full evidence base. Direction-of-effect moderator patterns indicated a stepped severity gradient (indicated 100% favorable, selective 98.6%, universal 95.6%), and that programs exceeding 8 weeks (99.0% vs. 96.6%), theory-based interventions (98.2% vs. 95.2%), and guided digital delivery were consistently associated with the most favorable outcomes. Publication bias assessment confirmed robustness (fail-safe N = 22,942; leave-one-out range: 0.61–0.67). GRADE evidence quality was rated Moderate for four of five research questions. Conclusions: This systematic review and meta-analysis provide converging quantitative and direction-of-effect evidence supporting the effectiveness of psychoeducational interventions. The near-universal favorable direction across 186 studies, combined with a medium-to-large pooled effect in the quantitative subset, provides a preliminary empirical foundation for personalized intervention matching. A preliminary four-phase implementation framework is proposed as a hypothesis-generating heuristic; prospective validation through a meta-analysis of individual participant data is needed before prescriptive application. Full article

Background: Lifestyle behaviours and medication adherence are interrelated components of cardiovascular secondary prevention, yet their co-occurrence in Middle Eastern post-percutaneous coronary intervention (PCI) populations remains poorly characterised. This study described smoking status and physical activity patterns, assessed antiplatelet medication adherence, and explored associations between lifestyle factors and adherence among Saudi patients following PCI. Methods: A cross-sectional survey was conducted among 236 Saudi adults who had undergone PCI within the preceding 12 months at two tertiary cardiac centres in Riyadh, Saudi Arabia. Data were collected on smoking status, cigarette consumption, self-reported physical activity frequency (defined as the frequency of engagement in regular exercise such as walking, swimming, or other structured physical activity), and self-reported medication adherence measured via the Morisky Medication Adherence Scale-8 (MMAS-8). Descriptive statistics characterised lifestyle and adherence patterns. Bivariate analyses (chi-square tests) and multivariate binary logistic regression were used to explore associations between lifestyle factors and adherence, adjusting for comorbidities including hypertension, diabetes mellitus, arthritis, and heart disease. Results: Participants were predominantly male (73.7%) and older adults (83.9% aged >50 years). Smoking prevalence was 23.3% (n = 55 of 236 participants), and physical inactivity was highly prevalent, with 57.2% of the sample (n = 135) reporting never engaging in regular exercise. Adherence was suboptimal, with 55.4% of participants (n = 129) classified as having low adherence (self-reported, measured via the MMAS-8). In multivariate analysis, arthritis was the only statistically significant predictor of adherence (adjusted odds ratio [AOR] = 2.81, 95% confidence interval [CI]: 1.01–7.84, p = 0.048; note, however, that this finding does not survive Bonferroni correction for multiple comparisons and should be interpreted as hypothesis-generating). Smoking (AOR = 0.52, 95% CI: 0.19–1.45, p = 0.213) and physical inactivity (AOR = 0.45, 95% CI: 0.09–2.25, p = 0.332) showed inverse but statistically non-significant trends with adherence. Conclusions: Unhealthy lifestyle behaviours and low medication adherence were each highly prevalent and co-occurred in this post-PCI population, though associations between lifestyle factors and adherence were not statistically confirmed except for arthritis. These descriptive findings are hypothesis-generating and provide a rationale for future adequately powered prospective studies and trials evaluating integrated nursing interventions that simultaneously address smoking, physical inactivity, and medication non-adherence in Saudi cardiac care settings. Full article

N. chui is an economically important marine fish species distributed along the coastal waters of China, renowned for its delicate flesh texture and high-quality dried swim bladder. However, its scientific name and taxonomic relationship with N. coibor have long remained controversial, hindering accurate resource assessment and germplasm management. To address this issue, we sequenced and annotated the first complete mitochondrial genome of N. chui (GenBank accession: PZ024444). The circular mitogenome is 16,504 bp in length and contains 37 typical genes, with gene arrangement, nucleotide composition (A + T content: 52.07%), and codon usage patterns consistent with general teleost characteristics. Phylogenetic analyses based on 13 concatenated protein-coding genes revealed that N. chui and N. coibor form a maximally supported monophyletic clade (bootstrap support = 100%), with a pairwise genetic distance of 0. These mitochondrial results strongly suggest that the two nominal taxa are very closely related and may represent the same species. However, formal taxonomic synonymy cannot be established on mitochondrial evidence alone and requires further evaluation through examination of type material and comparative morphological study. Gene-specific selection pressure analyses showed that most mitochondrial protein-coding genes were subject to purifying selection, with ATP8 exhibiting the highest mean ω among genes with ω < 1, whereas ND5 and ND6 showed elevated ω values that warrant cautious interpretation. This study provides essential mitochondrial genomic resources for future research on species delimitation, phylogeny, and conservation of this important sciaenid fish. Full article

The global spread of high-risk clo1nes (HRCs) of multidrug-resistant (MDR) Pseudomonas aeruginosa has hindered infection control and treatment strategies worldwide. In Colombia, globally relevant HRCs such as ST235 and ST111 have been widely reported. In this study, we evaluated phenotypic and metabolic variations associated with intracellular survival and dissemination in P. aeruginosa. A total of 100 clinical isolates were collected from 22 hospitals in Colombia. The isolates had been previously characterized and classified as MDR or susceptible strains (SSs), and their sequence types (STs) had been earlier determined. Based on this prior characterization, isolates were grouped in this study as multidrug-resistant high-risk clones (HRC, n = 50; corresponding to sequence types ST235 and ST111), multidrug-resistant non-high-risk clones (NHRCs, n = 27; non-ST235/ST111), and susceptible strains (SS, n = 23; also, non-ST235/ST111). Phenotypic traits, including motility, spontaneous mutation frequency, biofilm formation, and pigment production, were evaluated. In addition, a subset of 30 isolates was assessed for intracellular survival in vitro and metabolomic profiling using liquid chromatography-mass spectrometry. HRC isolates exhibited significantly reduced motility compared with NHRC and SS isolates (swarming: HRC vs. NHRC, p = 0.0032; HRC vs. SS, p = 0.010; swimming: HRC vs. NHRC and SS, p < 0.0001; twitching: HRC vs. SS, p = 0.0004), as well as lower pigment production (pyocyanin: HRC vs. NHRC and SS, p < 0.0001; pyoverdine: HRC vs. NHRC, p < 0.0001). Metabolomic analysis revealed increased concentrations of metabolites associated with iron acquisition and siderophore-related pathways in HRC isolates. Overall, these findings suggest that P. aeruginosa HRCs display distinct phenotypic and metabolic patterns that may contribute to persistence and dissemination in clinical settings, contributing to their epidemiological success. Full article