Search Results (164)

Bryophytes’ adaptability and stress resistance make them excellent colonizers. Moreover, bryophytes are key components of almost all terrestrial ecosystems from aquatic to arid to freezing cold. Bryophytes are also unique models to study adaptation and stress resistance in plants. Bryological studies in the Mediterranean area are mainly floristic-oriented, and consequently, the knowledge of the autoecology of the species inhabiting Mediterranean islands and islets is very scarce. The aim of this study is to evaluate bryophyte diversity in a number of islands and islets surrounding the Italian peninsula, Sicily and Sardinia. Moreover, based on the geographical and environmental features available for the studied islands, we analyze the role of different ecological variables (such as the islands’ area, insularity degree, altitude, and substratum type) in shaping bryophytic species richness and diversity. In the present study, ecological indicators adapted to Mediterranean bryophytes were also used to describe from an ecological and functional viewpoint the species inhabiting the studied islands and to explore to what extent the islands’ features have had a role in shaping the ecological features of the bryophytic floras inhabiting them. Within this study, an updated overview on the floristic richness and diversity of the small islands surrounding the Italian peninsula, Sicily and Sardinia was presented. The subject of the discussion was the island-related floristic and ecological differences as well as the drivers of these differences. Full article

A dendrochronological study was conducted on a submontane raised bog, Bór na Czerwonem, in the Orava–Nowy Targ Basin in Southern Poland. In the past, the bog was drained to enable peat extraction. In recent years, a number of measures considered as active protection were undertaken, including the construction of ridges and locks, filling of the drainage trenches, and clearance of most of the tree stand on the bog dome. Pinus sylvestris, P. × rhaetica, and P. mugo were the focuses of the study, which aimed to determine the age of the genus stand and its age structure and to identify the factors influencing tree ring width. The age of the trees indicates a post-war succession induced by large-scale drainage in 1942, although single trees were present on the bog dome as early as the late 19th century, and probably earlier. High values of pith eccentricity at ground level testify to substratum instability and the impact of strong winds on tree ring formation. The growth–climate relationships change with the progressive climate change: the significance of insolation increases, while the significance of the absolute air temperature decreases. The thermal and pluvial conditions of the summer in the previous growth season, however, make the strongest impact on the tree ring width in the following growth season. The health of the trees left growing on the bog, due to the constantly rising water level, will likely deteriorate, and a decreasing number of seedlings will be observed. A full assessment of the conducted restoration efforts, however, will be possible after years of monitoring of the bog environment. Full article

Alcalá de Ebro is located 35 km northwest of the city of Zaragoza, on the right bank of the Ebro River at the outlet of a ravine (Juan Gastón) towards the river, with a catchment area of more than 230 km². Over time, urbanisation and agricultural development have eliminated the last stretch of the drainage channel, and these water inputs have been channelled underground, filtering through the ground. This section of the Ebro Valley rests on a marly tertiary substratum, which promotes dissolution-subbing processes that can lead to sinkholes. The ground tends to sink gradually or suddenly collapse. Many studies have been carried out to understand not only the origin of the phenomenon but also its geometry and the area affected by it in the town of Alcalá de Ebro. In this sense, it has been possible to model an area around the main access road, where numerous collapsing sinkholes have been found, blocking the road and affecting houses. It also affects the embankment that protects the town from the floods of the river Ebro. These studies have provided specific knowledge, enabling us to evaluate and implement underground consolidation measures, which have shown apparent success. Several injection campaigns have been carried out, initially with expansion resins and finally with columnar development, using special low-mobility mortars to fill and consolidate the undermined areas and prevent new subsidence. These technical solutions propose a method of ground treatment that we believe is novel for this type of geological process. The results have been satisfactory, but it is considered necessary to continue monitoring the situation and to extend attention to a wider area to prevent, as far as possible, new problems of subsidence and collapse. In this sense, the objective is to continue the control and monitoring of possible phenomena related to subsidence problems in the affected area and its immediate surroundings, to detect and, if necessary, anticipate subsidence or collapse phenomena that could affect the body of the embankment. Full article

In order to design antimicrobial species, a series of methacrylate (Macr) complexes, [Cu(HBzIm)₂(Macr)₂] (1), [Cu₂(HBzIm)₂(Macr)₄] (2), [Cu(2-MeBzIm)₂(Macr)₂] (3), [Cu₂(2-MeBzIm)₂(Macr)₄] (4), and [Cu(5,6-Me₂BzIm)₂(Macr)₂] (5) (HBzIm = benzimidazole, 2-MeBzIm = 2-methylbenzimidazole, and 5,6-Me₂BzIm = 5,6-dimethylbenzimidazole) were synthesized and characterized by several spectral techniques, as well as by single crystal X-ray diffraction. The mononuclear species exhibit a distorted octahedral stereochemistry, while the binuclear types, with a paddle-wheel structure, adopt a square pyramidal surrounding. The methacrylate acts either as a chelate or a bridge, while all benzimidazole derivatives are coordinated as unidentate. The supramolecular networks are developed by both intermolecular π–π stacking interactions and hydrogen bonds. The antimicrobial assays provided both complexes the ability to inhibit planktonic strain proliferation, as well as to adhere on inert substratum. All complexes exhibit a moderate antimicrobial activity, both in regards to standard and clinical isolate strains, the most active being compound 5 against Candida albicans, with a minimum inhibitory concentration (MIC) of 0.156 mg/mL. It is worth mentioning that complex 1 inhibited the microbial adhesion of the clinical Escherichia coli strain and complex 2 constrained that of the clinical C. albicans strain. Full article

Industrially applied bioelectrochemical systems require long-term stable operation, and hence the control of biofilm accumulation on the electrodes. An optimized application of biofilm control mechanisms presupposes on-line, in-situ monitoring of the accumulated biofilm. Heat transfer sensors have successfully been integrated into industrial systems for on-line, non-invasive monitoring of biofilms. In this study, a mathematical model for the description of the sensitivity of a heat transfer biofilm sensor was developed, incorporating the hydrodynamic conditions of the fluid and the geometrical properties of the substratum. This model was experimentally validated at different flow velocities by integrating biofilm sensors into cylindrical pipes and planar mesofluidic flow cells with a carbonaceous substratum. Dimensionless sensor readings were correlated with the mean biovolume measured gravimetrically, and optical coherence tomography was used to determine the sensors’ sensitivity. The biofilm sensors applied in the planar flow cells revealed an increase in sensitivity by a factor of 6 compared to standard stainless steel pipes, as well as improved sensitivity at higher flow velocities. Full article

Western Tianshan National Nature Reserve in Xinjiang, China stands out for its uniqueness and high biodiversity, including lichens. This study aims to characterize lichen diversity and compare distribution patterns of different life forms, substratum affinities and photobiont types. Surveys were conducted from June to August 2024 using stratified sampling methods at elevation ranging from 1100 m to 3400 m in the study area. Morphological, anatomical and chemical studies revealed 173 lichen species from 24 families and 58 genera, of which 100 species were identified as crustose, 46 as foliose and 27 as fruticose. Among the different habitat groups, strictly saxicolous lichens were dominant with 89 species, followed by corticolous lichens with 44 species and terricolous lichens with 40 species. The total species richness of lichens has a bimodal pattern: one peak appears at a low altitude (1701–2000 m) and the other at a high altitude (2901–3200 m). Among the three substratum categories studied, the species richness of terricolous lichens showed a unimodal relationship with elevation, and the saxicolous lichen had a bimodal pattern. The species richness of corticolous lichens was highest at lower and medium elevations and decreased at higher elevations. With respect to photobiont type, the species richness of cyanolichens showed a unimodal relationship with elevation. Maximum richness occurred at 2700 m, contrary to the chlorolichens, which had a bimodal pattern. Species richness of all three growth forms of lichens showed a bimodal pattern related to elevation. Among the three morphological types, crustose and foliose species richness had their highest values of 38 and 19, respectively, at 1701–2000 m, and fruticose lichens peaked with a maximum of 13 species at 2301–2600 m. The species richness of crustose lichens is lowest between altitudes 2300 and 2600 m, while the lowest species richness of fruticose and foliose lichens occurs at 2001–2300 m and elevations above 2900 m. Full article

A game problem of structural design is defined as a problem of playing against external circumstances. There are two classes of players, namely the “ordinal” and “cardinal” players. The ordinal players, designated as the “operator” and “nature”, endeavor to, respectively, minimize or maximize the payoff function, operating within the constraints of limited resources. The fundamental premise of this study is that the action of player “nature” is a priori unknown. Statistical decision theory addresses decision-making scenarios where these probabilities, whether or not they are known, must be considered. The solution to the substratum game is expressed as a value of the game “against nature”. The structural optimization extension of the game considers the value of the game “against nature” as the function of certain parameters. Thus, the value of the game is contingent upon the design parameters. The cardinal players, “designers”, choose the design parameters. There are two formulations of optimization. For the single cardinal player, the pursuit of the maximum and minimum values of the game reduces the problem of optimal design. In the second formulation, there are multiple cardinal players with conflicting objectives. Accordingly, the superstratum game emerges, which addresses the interests of the superstratum players. Finally, the optimal design problems for games with closed forms are presented. The game formulations could be applied for optimal design with uncertain loading, considering “nature” as the source of uncertainty. Full article

The large-scale dieback of spruce monocultures, especially in the lower alpine, has become a significant problem and has necessitated the restoration of these areas, mainly using seedlings produced in forest nurseries. The primary source of nutrients for seedlings can be slow-release fertilizers and an appropriate dose of fertilizer improves the efficiency of its use and minimizes the negative environmental impact associated with the excessive use of mineral fertilizers. Aims: This study aimed to evaluate the effect of applying different fertilizer dose combinations on the accumulation of macronutrients in different parts of the seedlings (roots, shoots, and leaves) and on the morphology and development of fine roots. Methods: This research was carried out on producing beech seedlings with the application of starter soil fertilization with Yara Mila Complex (YMC) and Osmocote Exact Standard 3-4M (OES) fertilizers in four varying doses. Results: No deficiency of the analyzed macronutrients was noted in any of the tested fertilization variants. The highest content of all analyzed macronutrients was recorded in the leaves of beech seedlings, with values in roots and shoots being several times lower. The mixed fertilization variant OES 1.0 + YMC 1.0 shows a positive correlation with all analyzed elements and the parameters DQI (Dickson Quality Index), SA (Surface Area), RV (Root Volume), and mass. Conclusions: Results confirm the hypothesis that applying a mixture of fast-acting (YMC) and slow-acting (OES) fertilizer positively affects the nutrition and accumulation of macronutrients and the development of root systems in beech seedlings compared to fertilization with a single fertilizer. Full article

To effectively manage the sustainable urban development of cities, it is crucial to quickly understand the geological and geotechnical attributes of the underground. Carrying out such studies entails significant investments and focused reconnaissance efforts, which might not align seamlessly with large-scale territorial planning initiatives within a city accommodating more than 3 million inhabitants, like Casablanca in Morocco. Additionally, various specific investigations have been conducted by municipal authorities in recent times. The primary aim of this study is to furnish city managers and planners with a tool for informed decision-making, enabling them to explore the geological and geotechnical properties of soil foundations using Geographic Information Systems (GISs) and geostatistics. This database, initially intended for utilization by developers and construction engineers, stands to economize a substantial amount of time and resources. During the urban planning of cities and prior to determining land usage (five- or seven-floor structures), comprehending the mechanical traits (bearing capacity, water levels, etc.) of the soil is crucial. To this end, geological and geotechnical maps, along with a collection of 100 surveys, were gathered and incorporated into a GIS system. These diverse data sources converged to reveal that the underlying composition of the surveyed area comprises silts, calcarenites, marls, graywackes, and siltstones. These formations are attributed to the Middle Cambrian and the Holocene epochs. The resultant geotechnical findings were integrated into the GIS and subjected to interpolation using ordinary kriging. This procedure yielded two distinct maps: one illustrating bearing capacity and the other depicting the substratum. The bearing capacity of the soil in the study zone is rated as moderate, fluctuating between two and four bars. The depth of the foundation remains relatively shallow, ranging from 0.8 m to 4.5 m. The outcomes are highly promising, affirming that the soil in Casablanca boasts commendable geotechnical attributes capable of enduring substantial loads and stresses. Consequently, redirecting future urban planning in the region toward vertical expansion seems judicious, safeguarding Casablanca’s remaining green spaces and the small agricultural belt. The results of this work help to better plan the urban development of the city of Casablanca in a smarter way, thus preserving space, agriculture, and the environment while promoting sustainability. In addition, the databases and maps created through this paper aim for a balanced financial management of city expenditures in urban planning. Full article

Campylobacter spp. are prevalent foodborne bacterial enteric pathogens. Their inclusion in biofilms on abiotic surfaces is considered a strategy that facilitates their extraintestinal survival. Organic acid (OA) treatments could be used in a green approach to decontaminate various surfaces. This work aimed to evaluate the inhibitory and eradicative effects of L(+)-lactic acid (LA), a naturally occurring OA, on a dual-species biofilm formed on two food processing model surfaces (polystyrene and stainless steel) by three selected foodborne Campylobacter spp. isolates (two C. jejuni and one C. coli). The influence of aerobiosis conditions (microaerophilic, aerobic and CO₂ enriched) on the resistance of the established biofilms to the acid was also tested. In parallel, the predominant metabolites contained in the planktonic media of biofilm monocultures and mixed-culture biofilm were comparatively analyzed by an untargeted metabolomics approach. Results revealed that LA inhibited mixed-culture biofilm formation by more than 2 logs (>99%) on both surfaces when this was applied at its highest tested concentration (4096 μg/mL; 0.34% v/v). However, all the preformed mixed-culture biofilms (ca. 10⁶⁻⁷ CFU/cm²) could not be eradicated even when the acid was used at concentrations exceeding 5% v/v, denoting their extremely high recalcitrance which was still influenced by the abiotic substratum, and the biofilm-forming aerobiosis conditions. The metabolic analysis revealed a strain-specific metabolite production which might also be related to the strain-specific biofilm-forming and resistance behaviors and resulted in the distinct clustering of the different samples. Overall, the current findings provide important information on the effectiveness of LA against biofilm campylobacteria and may assist in mitigating their risk in the food chain. Full article

Studies on the Indo-European principle of trifunctionality and on the medieval Three Estates imagery provide researchers with precious resources for investigating the ways and means through which social representations become loaded with sacredness, and sacredness progressively transferred from one set of social representations to another. This article focuses on the transformations undergone by the Three Estates religious substratum, first at the time of the birth of Absolutism, and second during and after the French Revolution. In the case of France (and with openings towards other contexts), it shows how trifunctionality has been attributed, alternatively or conjointly, to the bourgeoisie and to the nation. Triune imagery has thus contributed to shape rituals and symbols proper to civil religion in France. Additionally, this contribution draws lessons from our case study for assessing both the resilience and the power of metamorphosis proper to symbolic references once the latter are organized into patterns that nurture a community’s cohesiveness by the putting into motion of images, narratives and ritual practices. Full article

The ability of bacteria to colonize diverse environmental niches is often linked to their competence in biofilm formation. It depends on the individual characteristics of a strain, the nature of the colonized surface (abiotic or biotic), or the availability of certain nutrients. Pseudomonas donghuensis P482 efficiently colonizes the rhizosphere of various plant hosts, but a connection between plant tissue colonization and the biofilm formation ability of this strain has not yet been established. We demonstrate here that the potential of P482 to form biofilms on abiotic surfaces and the structural characteristics of the biofilm are influenced by the carbon source available to the bacterium, with glycerol promoting the process. Also, the type of substratum, polystyrene or glass, impacts the ability of P482 to attach to the surface. Moreover, P482 mutants in genes associated with motility or chemotaxis, the synthesis of polysaccharides, and encoding proteases or regulatory factors, which affect biofilm formation on glass, were fully capable of colonizing the root tissue of both tomato and maize hosts. Investigating the role of cellular factors in biofilm formation using these plant-associated bacteria shows that the ability of bacteria to form biofilm on abiotic surfaces does not necessarily mirror its ability to colonize plant tissues. Our research provides a broader perspective on the adaptation of these bacteria to various environments. Full article

Biofilms play a decisive role in the infectious process and the development of antibiotic resistance. The establishment of bacterial biofilms is regulated by a signal-mediated cell–cell communication process called “quorum sensing” (QS). The identification of quorum sensing inhibitors (QSI) to mitigate the QS process may facilitate the development of novel treatment strategies for biofilm-based infections. In this study, the traditional medicinal plant Ocimum sanctum was screened for QS inhibitory potential. Sub-MICs of the extract significantly affected the secretion of EPS in Gram-negative human pathogens such as Escherichia coli, Pseudomonas aeruginosa PAO1, Proteus mirabilis, and Serratia marcescens, as well as aquaculture pathogens Vibrio harveyi, V. parahaemolyticus, and V. vulnificus, which render the bacteria more sensitive, leading to a loss of bacterial biomass from the substratum. The observed inhibitory activity of the O. sanctum extract might be attributed to the presence of eugenol, as evidenced through ultraviolet (UV)-visible, gas chromatography-mass spectroscopy (GC–MS), Fourier transformer infrared (FTIR) spectroscopy analyses, and computational studies. Additionally, the QSI potential of eugenol was corroborated through in vitro studies using the marker strain Chromobacterium violaceum. Full article

There is limited information regarding the influence of mangrove growth on the morphological evolution of intertidal mudflats. In this study, Tan Phu Dong district, Tien Giang Province, Vietnam, was selected for investigating how mangrove growth influenced the morphological evolution of an intertidal mudflat. The authors analyzed a series of satellite images (from 1995 and 2022), calculated the enhanced vegetation index (EVI), and documented field visits and observations in pursuit of the objective of the study. The findings revealed that fine-grained sediment accumulated as unconsolidated substratum (US) in the first step of the morphological evolution of the intertidal mudflat, with sediment accumulation of 910 ha in 1995. The US provided favorable conditions for mangroves to grow, while mangrove growth helped compact the US into a compact substratum (CS) in addition to promoting continuous sediment accumulation, increased the vegetation cover of the island, and elevated the substrate density of the remaining areas. As a result, the US and CS decreased steadily between 1995 and 2020, from 910 ha in 1995 to 401 ha in 2020 and from 433 ha in 2005 to 111 ha in 2020, respectively. Meanwhile, the low-vegetation area (LVA), medium-vegetation area (MVA), and high vegetation area (HVA) gradually increased between 1995 and 2015, from 0 ha in 1995 to 104 ha in 2015, from 0 ha in 1995 to 96 ha in 2015, and from 0 ha in 1995 to 114 ha in 2015, respectively. However, the LVA decreased slightly between 2015 and 2020 due to significant sand accumulation, which significantly killed the mangrove trees. In contrast, the MVA and HVA steadily increased between 2015 and 2020, from 96 ha in 2015 to 116 ha in 2020 and from 114 ha in 2015 to 221 ha in 2020, respectively. In 2022, there was a steady increase in HVA (298 ha in 2022), although the date of the 2022 satellite retrieval was 28 January 2022. This study recommends that the technical design of the existing coastal protection works should be revised or adapted to take account of sediment accumulation as the first step in the morphological evolution of the examined intertidal mudflat, rather than mangrove growth. Full article

In the majority of Abhidharmic Buddhist schools, smṛti serves the crucial function of ensuring the recollection of past events and is thus conceptualized as memory in scholarly discourse nowadays. Nevertheless, upon closer examination of various doctrinal perspectives, the interpretation of smṛti diverges significantly among different schools. Notably, it is deemed to be omnipresent in every moment of perception within the Sarvāstivādin tradition, while in Yogācāra, it is regarded as a specific response to an experienced object. This paper seeks to delve into the concept of smṛti within the latter tradition, with a specific focus on the Cheng Weishi Lun (成唯識論, henceforth: CWSL). Drawing upon the foundational principles of Yogācāra doctrine, which posit the ālayavijñāna as an underlying substratum responsible for retaining past information, I contend that smṛti embodies a cognitive faculty that intensifies the apprehension of learned objects when they are present. Furthermore, I argue that smṛti serves as the inception point for a series of cognitive processes acquiring knowledge that helps decision-making. Additionally, I will demonstrate that the function of smṛti in the CWSL diverges from both traditional Buddhist conceptions of memory and contemporary understandings thereof. Full article