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Keywords = simultaneous pulse oscillation

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18 pages, 3889 KB  
Article
Proposal of Difference-Frequency Wave Generation Induced by Dual-Wavelength Free-Electron Laser Oscillations
by Norihiro Sei, Takeshi Sakai, Heishun Zen and Hideaki Ohgaki
Photonics 2026, 13(4), 344; https://doi.org/10.3390/photonics13040344 - 1 Apr 2026
Viewed by 491
Abstract
Optical klystrons have been developed in storage ring free-electron lasers (FELs) as insertion devices to increase the FEL gain in a straight section with limited length. By adjusting the magnetic field in the dispersion section of the optical klystron to shift the relative [...] Read more.
Optical klystrons have been developed in storage ring free-electron lasers (FELs) as insertion devices to increase the FEL gain in a straight section with limited length. By adjusting the magnetic field in the dispersion section of the optical klystron to shift the relative delay between the electron bunch and FEL pulse from an integer multiple of the FEL wavelength, FELs can oscillate at two wavelengths. The electron density of the electron bunch that interacts with the FEL pulse in a small-signal regime is modulated at the FEL wavelength period. When the FEL oscillates simultaneously at two wavelengths, the electron density of the electron bunch beats through the modulation with two periods. This beat generates long-wavelength coherent edge radiation at a bending magnet located in the straight section containing the optical klystron. Difference-frequency waves induced by dual-wavelength ultraviolet free-electron lasers generate a high-intensity mid-infrared monochromatic beam. Our findings will lay the foundation for the development of the difference-frequency waves of soft X-rays and extreme ultraviolet light using hard X-ray FELs. Full article
(This article belongs to the Section Lasers, Light Sources and Sensors)
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16 pages, 1786 KB  
Article
Integrating High-Capacity Self-Homodyne Transmission and High-Sensitivity Dual-Pulse ϕ-OTDR with an EO Comb over a 7-Core Fiber
by Xu Liu, Chenbo Zhang, Yi Zou, Zhangyuan Chen, Weiwei Hu, Xiangge He and Xiaopeng Xie
Photonics 2026, 13(3), 261; https://doi.org/10.3390/photonics13030261 - 9 Mar 2026
Viewed by 459
Abstract
Beyond supporting ultra-high-capacity data transmission, metropolitan and access networks are expected to enable real-time infrastructure monitoring, driving the emergence of integrated sensing and communication (ISAC). Distributed acoustic sensing (DAS) has proven to be well-suited to urban sensing application requirements, yet its seamless integration [...] Read more.
Beyond supporting ultra-high-capacity data transmission, metropolitan and access networks are expected to enable real-time infrastructure monitoring, driving the emergence of integrated sensing and communication (ISAC). Distributed acoustic sensing (DAS) has proven to be well-suited to urban sensing application requirements, yet its seamless integration into ISAC remains challenging—conventional high-peak-power sensing pulses in DAS induce nonlinear crosstalk in communication channels. DAS inherently suffers from interference fading due to single-frequency laser sources, which limits sensitivity. Here, we propose an ISAC architecture based on an electro-optic (EO) comb and a 7-core fiber, achieving nonlinearity-suppressed self-homodyne transmission and fading-suppressed DAS. Unmodulated comb lines and sensing pulses are polarization-multiplexed into orthogonal polarization states within the central core to minimize nonlinear crosstalk while delivering local oscillators (LOs) for wavelength division multiplexing (WDM) coherent transmission within six outer cores—achieving 10.56 Tbit/s capacity. In addition to supporting WDM transmission, the EO comb’s wavelength diversity is also exploited to enhance DAS performance. Specifically, a dual-pulse probe loaded onto four comb lines yields a 6 dB signal-to-noise ratio gain and a 64% reduction in fading occurrences, achieving a sensitivity of 1.72 pε/Hz with 8 m spatial resolution. Moreover, our system supports simultaneous multi-wavelength backscatter detection in sensing and simplified digital signal processing in self-homodyne communication, reducing receiver complexity and cost. Our work presents a scalable, energy-efficient ISAC framework that unifies high-capacity communication with high-sensitivity sensing, providing a blueprint for future intelligent optical networks. Full article
(This article belongs to the Special Issue Next-Generation Optical Networks Communication)
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16 pages, 953 KB  
Review
A Review of Differential Plant Responses to Drought, Heat, and Combined Drought + Heat Stress
by Nankai Li, Zhi Geng, Xiaodong Huang, Shunqi Huang, Lulu Song, Ruirui Chen, Ziping Chen, Liji Du and Congshan Xu
Curr. Issues Mol. Biol. 2025, 47(12), 975; https://doi.org/10.3390/cimb47120975 - 24 Nov 2025
Cited by 4 | Viewed by 2340
Abstract
Global warming increases the frequency with which drought and heat stress occur simultaneously, especially in semi-arid regions. Such combined stress imposes a non-additive and more severe impact on plant growth, yield, and quality than either stress alone. Here, we integrate recent physiological, biochemical, [...] Read more.
Global warming increases the frequency with which drought and heat stress occur simultaneously, especially in semi-arid regions. Such combined stress imposes a non-additive and more severe impact on plant growth, yield, and quality than either stress alone. Here, we integrate recent physiological, biochemical, and multi-omics studies to compare individual and combined stress responses and to dissect the underlying signal transduction networks. We show that drought-dominated phases rapidly elevate ABA concentrations and activate SnRK2–AREB cascades, whereas heat pulses trigger jasmonic acid and ethylene signals that antagonize ABA-driven stomatal closure. Under combined stress, these hormonal modules converge on a “competitive TF marketplace”, where ABA, JA, and GA cis-elements co-regulate invertase–sugar checkpoints, heat shock factor/ROS oscillators, and chromatin-remodeling events that determine reproductive fate. Recent advances using multi-omics approaches and systems biology have further elucidated these complex networks. These insights will inform future breeding strategies aiming to develop stress-tolerant crops. We highlight emerging tools—weighted gene co-expression networks, kinetic multi-omics, and cis-regulatory CRISPR editing—that can exploit these signaling hubs for breeding crops with improved combined stress tolerance. Full article
(This article belongs to the Section Molecular Plant Sciences)
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17 pages, 4074 KB  
Article
A Platform Integrating Biophysical and Biochemical Stimuli to Enhance Differentiation and Maturation of Cardiomyocyte Subtypes Derived from Human Induced Pluripotent Stem Cells
by Zhonggang Feng, Kota Sawada, Iori Ando, Riku Yoshinari, Daisuke Sato and Tadashi Kosawada
J. Cardiovasc. Dev. Dis. 2025, 12(2), 56; https://doi.org/10.3390/jcdd12020056 - 4 Feb 2025
Cited by 2 | Viewed by 1805
Abstract
To enhance the differentiation and maturation of cardiomyocytes derived from human induced pluripotent stem cells, we developed a bioreactor system that simultaneously imposes biophysical and biochemical stimuli on these committed cardiomyocytes. The cells were cultured within biohydrogels composed of the extracellular matrix extracted [...] Read more.
To enhance the differentiation and maturation of cardiomyocytes derived from human induced pluripotent stem cells, we developed a bioreactor system that simultaneously imposes biophysical and biochemical stimuli on these committed cardiomyocytes. The cells were cultured within biohydrogels composed of the extracellular matrix extracted from goat ventricles and purchased rat-origin collagen, which were housed in the elastic PDMS culture chambers of the bioreactor. Elastic and flexible electrodes composed of PEDOT/PSS, latex, and graphene flakes were embedded in the hydrogels and chamber walls, allowing cyclic stretch and electrical pulses to be simultaneously and coordinately applied to the cultured cells. Furthermore, a dynamic analysis method employing the transverse forced oscillation theory of a cantilever was used to analyze and discriminate the subtype-specific beating behavior of the cardiomyocytes. It was found that myosin light chain 2v (MLC2v), a ventricular cell marker, was primarily upregulated in cells aggregated on the (+) electrode side, while cardiomyocytes with faint MLC2v but strong cardiac troponin T (cTNT) expression aggregated at the ground electrode (GND) side. mRNA analysis using rtPCR and the gel beating dynamics further suggested a subtype deviation on the different electrode sides. This study demonstrated the potential of our bioreactor system in enhancing cardiac differentiation and maturation, and it showed an intriguing phenomenon of cardiomyocyte subtype aggregation on different electrodes, which may be developed into a new method to enhance the maturation and separation of cardiomyocyte subtypes. Full article
(This article belongs to the Section Cardiac Development and Regeneration)
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22 pages, 7927 KB  
Article
The Design and Dynamic Control of a Unified Power Flow Controller with a Novel Algorithm for Obtaining the Least Harmonic Distortion
by Armel Asongu Nkembi, Nicola Delmonte, Paolo Cova and Minh Long Hoang
Electronics 2024, 13(5), 877; https://doi.org/10.3390/electronics13050877 - 24 Feb 2024
Cited by 4 | Viewed by 2662
Abstract
This study investigates the control and dynamic operation of the Unified Power Flow Controller made of shunt and series converters, a Static Synchronous Compensator, and a Static Synchronous Series Compensator, respectively, connected back-to-back through a common DC-link capacitor. The model of a 48-pulse [...] Read more.
This study investigates the control and dynamic operation of the Unified Power Flow Controller made of shunt and series converters, a Static Synchronous Compensator, and a Static Synchronous Series Compensator, respectively, connected back-to-back through a common DC-link capacitor. The model of a 48-pulse Voltage Source Converter is constructed from a three-level Neutral Point Clamped converter, which allows the total harmonic distortion to be reduced. An optimal conduction angle tracking system of the three-level inverter is designed to minimize distortion by detecting proper harmonic component elimination. Starting from the six-step modulation strategy, the dq decoupled control schemes of both compensators in open and closed loops are presented. Finally, the MATLAB-Simulink model of the power flow controller is implemented and analyzed. The results show that the controller can track the power changes and apply a suitable voltage to the power system so that the power flow can be controlled. This way, the power flow controller dynamically improves the voltage and power quality across the power network while simultaneously improving the transient stability of the system. It can eliminate all system disturbances resulting from oscillations and harmonics in voltage and current within a very short time. The procedural approach used to model and simulate the Unified Power Flow Controller, as well as the new algorithm used to obtain the harmonic number that minimizes the total harmonic distortion, can be applied to any AC power system. Full article
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14 pages, 4221 KB  
Article
A Novel Continuous-Variable Quantum Key Distribution Scheme Based on Multi-Dimensional Multiplexing Technology
by Shuai Zhang, Heng Wang, Yan Pan, Yun Shao, Tao Zhang, Wei Huang, Yang Li and Bingjie Xu
Appl. Sci. 2024, 14(2), 934; https://doi.org/10.3390/app14020934 - 22 Jan 2024
Cited by 1 | Viewed by 2519
Abstract
Dual-polarization division multiplexing (DPDM) is considered to be a potential method to boost the secure key rate (SKR) of the continuous-variable quantum key distribution (CV-QKD) system. In this article, we propose a pilot alternately assisted local local oscillator (LLO) CV-QKD scheme based on [...] Read more.
Dual-polarization division multiplexing (DPDM) is considered to be a potential method to boost the secure key rate (SKR) of the continuous-variable quantum key distribution (CV-QKD) system. In this article, we propose a pilot alternately assisted local local oscillator (LLO) CV-QKD scheme based on multi-dimensional multiplexing, where time division multiplexing and frequency division multiplexing are combined with dual-polarization multiplexing techniques to dramatically isolate the quantum signal from the pilot tone. We establish a general excess noise model for the LLO CV-QKD system to analyze the influence mechanism of various disturbances (e.g., time-domain diffusion, frequency-domain modulation residual, and polarization perturbation) on the key parameters, such as the channel transmittance and excess noise. Specifically, the photon leakage noise from the reference path to the quantum path and that between quantum signals with two different polarization paths are simultaneously analyzed in the dual-polarization LLO CV-QKD scheme for the first time. Furthermore, a series of simulations are established to verify the performance of the proposed scheme. The results show that the maximal isolation degree achieves 84.0 dB~90.4 dB, and the crosstalk between pilot tones and quantum signals can be suppressed to a very small range. By optimizing the system parameters (e.g., modulation variance and repetition frequency), the SKR with 12.801 Mbps@25 km is achieved under the infinite polarization extinction ratio (PER) and 30 dB residual ratio of the frequency modulation in the nanosecond-level pulse width. Moreover, the performance of the proposed DPDM CV-QKD scheme under relatively harsh conditions is simulated; the results show that the SKR with 1.02 Mbps@25 km is achieved under a relatively low PER of 17 dB with the nanosecond-level pulse width and 20 dB residual ratio of the frequency modulation. Our work lays an important theoretical foundation for the practical DPDM LLO CV-QKD system. Full article
(This article belongs to the Special Issue Advanced Technologies in Data and Information Security III)
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14 pages, 5195 KB  
Technical Note
The All-Solid-State Narrowband Lidar Developed by Optical Parametric Oscillator/Amplifier (OPO/OPA) Technology for Simultaneous Detection of the Ca and Ca+ Layers
by Lifang Du, Haoran Zheng, Chunlei Xiao, Xuewu Cheng, Fang Wu, Jing Jiao, Yuchang Xun, Zhishan Chen, Jiqin Wang and Guotao Yang
Remote Sens. 2023, 15(18), 4566; https://doi.org/10.3390/rs15184566 - 16 Sep 2023
Cited by 12 | Viewed by 3302
Abstract
We report an all-solid-state narrowband lidar system for the simultaneous detection of Ca and Ca+ layers over Yanqing (40.41°N, 116.01°E). The uniqueness of this lidar lies in its transmitter, which is based on optical parametric oscillation (OPO) and optical parametric amplification (OPA) [...] Read more.
We report an all-solid-state narrowband lidar system for the simultaneous detection of Ca and Ca+ layers over Yanqing (40.41°N, 116.01°E). The uniqueness of this lidar lies in its transmitter, which is based on optical parametric oscillation (OPO) and optical parametric amplification (OPA) techniques. The injection seeded OPO and the OPA are pumped by the second harmonic of an injection-seeded Nd:YAG laser, which can generate coherent light at the wavelength of 786 nm or 846 nm lasers, whose second harmonics in turn generate the 393 nm or 423 nm pulses, respectively, for the detection of thermospheric and ionospheric Ca+ and Ca layers. Compared to the conventional dye-based system, this lidar transmitter is a narrowband system (bandwidth < 200 MHz), which has produced a factor of two more output power with higher stability and reliability. The lidar system in Yingqing demonstrated Ca+ detection sensitivity of 0.1 atoms-cm−3 for long-term observation and reached a height of ~300 km. Potential applications and further improvements in this lidar technique are also discussed in this paper. Full article
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20 pages, 9457 KB  
Article
A Study and Implementation of Inductive Power Transfer System Using Hybrid Control Strategy for CC-CV Battery Charging
by Liangxi He, Xiaoqiang Wang and Chi-Kwan Lee
Sustainability 2023, 15(4), 3606; https://doi.org/10.3390/su15043606 - 15 Feb 2023
Cited by 12 | Viewed by 3518
Abstract
In this paper, a hybrid control strategy is studied and implemented on an Inductive Power Transfer (IPT) system to simultaneously realize zero-voltage switching (ZVS) and constant current (CC) and constant voltage (CV) battery charging. A steady-state analysis of pulse frequency modulation was conducted, [...] Read more.
In this paper, a hybrid control strategy is studied and implemented on an Inductive Power Transfer (IPT) system to simultaneously realize zero-voltage switching (ZVS) and constant current (CC) and constant voltage (CV) battery charging. A steady-state analysis of pulse frequency modulation was conducted, based on the characteristic of voltage gain versus switching frequency, and CC and CV charging modes were promised. The ZVS of the inverter was obtained by satisfying the minimum requirement of full discharge of the junction capacitor on the MOSFETs using a commutation current during the dead-time interval. Two control degrees of freedom are needed to realize the two control targets. This hybrid control strategy adopts a self-oscillating (SO) control to achieve ZVS and phase shift (PS) control and a constant output for the series–series (SS)-compensated IPT system. To validate the hybrid control strategy, a 1.6 kW prototype with 360–440 V input voltage and 250–400 V output voltage was built and the experimental results show that the peak efficiency can reach 96.1%. Compared with the conventional variable frequency (VF) control, the hybrid control method proves that an additional control variable can fulfill the control target in a more flexible manner, which makes the switching frequency close to the resonant frequency during the charging process, minimizing the reactive current in the resonant tank and improving system efficiency. Full article
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11 pages, 2606 KB  
Article
Distributed Impact Wave Detection in Steel I-Beam with a Weak Fiber Bragg Gratings Array
by Yuan Wang, Neil A. Hoult, Joshua E. Woods, Hannah Kassenaar and Xiaoyi Bao
Sensors 2023, 23(4), 2194; https://doi.org/10.3390/s23042194 - 15 Feb 2023
Cited by 3 | Viewed by 2755
Abstract
In this paper, acoustic, dynamic and static strain variations along a steel I-beam generated by an impact load are reconstructed simultaneously within a single measurement. Based on the chirped pulse φ-OTDR system with the single-shot measurement technique, both a higher strain-sensing resolution [...] Read more.
In this paper, acoustic, dynamic and static strain variations along a steel I-beam generated by an impact load are reconstructed simultaneously within a single measurement. Based on the chirped pulse φ-OTDR system with the single-shot measurement technique, both a higher strain-sensing resolution and a higher measurable vibration frequency are achieved. In addition, a weak fiber Bragg gratings array (WFBGA) with enhanced Rayleigh reflection is employed as a sensor, providing high signal-to-noise ratio Rayleigh traces, resulting in lower measurement uncertainty. In the experiments, the damping constant and fundamental frequency of the damped harmonic oscillator could then be measured based on the recovered strain variation profile for further structural health analysis. Compared with commercial strain gauges, linear potentiometers, and OFDR systems, the proposed sensing system ensures a distributed, quantitative, and high-frequency sensing ability, with an extensive range of potential applications. Full article
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13 pages, 4215 KB  
Article
Image-Rejected Multi-Band Frequency Down-Conversion Based on Photonic Sampling
by Liuzhu Xu, Di Peng, Yuwen Qin, Jianping Li, Meng Xiang, Ou Xu and Songnian Fu
Photonics 2023, 10(1), 35; https://doi.org/10.3390/photonics10010035 - 29 Dec 2022
Viewed by 3318
Abstract
An image-rejected multi-band frequency down-conversion scheme is proposed and experimentally demonstrated based on photonic sampling. The multi-band radio-frequency (RF) signals to be processed are copied into two replicas in quadrature, which are then sampled by an ultra-short optical pulse train via a polarization-multiplexed [...] Read more.
An image-rejected multi-band frequency down-conversion scheme is proposed and experimentally demonstrated based on photonic sampling. The multi-band radio-frequency (RF) signals to be processed are copied into two replicas in quadrature, which are then sampled by an ultra-short optical pulse train via a polarization-multiplexed modulator. After polarization demultiplexing and detection using a pair of low-speed photodetectors, the multi-band RF signals are simultaneously down-converted to the intermediate frequency (IF) band. The image components can be suppressed by quadrature coupling the two generated IF signals via an electrical 90° hybrid coupler (HC). In the experiment, multi-band RF signals in the frequency range of 6 GHz to 39 GHz are down-converted to the IF band below 4 GHz using a local oscillator (LO) signal at 8 GHz to generate the ultra-short optical pulse train. Image rejection is achieved in the digital domain using digital signal processing to compensate for the amplitude and phase mismatch between the two IF signals and to implement quadrature coupling. In addition, through using an electrical phase shifter, an electrical attenuator, and an electrical 90° HC to achieve quadrature coupling of the two IF signals, image-rejected multi-band frequency down-conversion is also verified in the analog domain. Full article
(This article belongs to the Special Issue Microwave Photonic Techniques)
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20 pages, 4678 KB  
Article
PCO-Based BLE Mesh Accelerator
by Ivan Bukreyev, Hazal Yüksel, Ken Ho and Alyssa Apsel
Sensors 2022, 22(14), 5324; https://doi.org/10.3390/s22145324 - 16 Jul 2022
Viewed by 2968
Abstract
Bluetooth Low Energy (BLE) mesh networks enable diverse communication for the Internet of Things (IoT). However, existing BLE mesh implementations cannot simultaneously achieve low-power operation, symmetrical communication, and scalability. A major limitation of mesh networks is the inability of the BLE stack to [...] Read more.
Bluetooth Low Energy (BLE) mesh networks enable diverse communication for the Internet of Things (IoT). However, existing BLE mesh implementations cannot simultaneously achieve low-power operation, symmetrical communication, and scalability. A major limitation of mesh networks is the inability of the BLE stack to handle network-scalable time synchronization. Pulse-coupled oscillators (PCOs) have been studied extensively and are able to achieve fast and reliable synchronization across a range of applications and network topologies. This paper presents a lightweight physical (PHY) layer accelerator to the BLE stack that enables scalable synchronization command with a PCO. The accelerator is a fully digital solution that can be synthesized with only the standard cells available in any silicon technology. This paper provides a detailed analysis of PCO-based BLE mesh networks and explores per-node system-level requirements. Finally, the analytical results are validated with measurements of a custom radio node based on the ubiquitous AD9364 transceiver. Full article
(This article belongs to the Section Internet of Things)
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12 pages, 2816 KB  
Article
A Hybrid Self-Seeded Ti:sapphire Laser with a Pumping Scheme Based on Spectral Beam Combination of Continuous Wave Diode and Pulsed DPSS Lasers
by Volker Sonnenschein and Hideki Tomita
Appl. Sci. 2022, 12(9), 4727; https://doi.org/10.3390/app12094727 - 7 May 2022
Cited by 5 | Viewed by 4200
Abstract
A wide variety of applications require high peak laser intensity in conjunction with a narrow spectral linewidth. Typically, injection-locked amplifiers have been employed for this purpose, where a continuous wave oscillator is amplified in a secondary external resonant amplifier cavity using a pulsed [...] Read more.
A wide variety of applications require high peak laser intensity in conjunction with a narrow spectral linewidth. Typically, injection-locked amplifiers have been employed for this purpose, where a continuous wave oscillator is amplified in a secondary external resonant amplifier cavity using a pulsed pump laser. In contrast, here we demonstrate a setup that combines a CW Ti:sapphire oscillator and pulsed amplifier in a single optical cavity, resulting in a compact system. Dichroic beam combination of blue wavelength semiconductor diodes and the green wavelength of a Nd:YAG laser allowed the simultaneous excitation of the Ti:sapphire crystal by both continuous wave and pulsed pump sources. A linewidth of <2 MHz is achieved in continuous wave operation, while the linewidth increases to about 10 MHz in the combined CW+pulsed mode with a pulse duration of 73 ns. A peak pulse intensity of 0.2 kW is achieved, which should enable efficient single-pass second harmonic generation in a nonlinear crystal. Full article
(This article belongs to the Special Issue Ti:Sapphire Lasers and Their Applications)
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14 pages, 3830 KB  
Article
Stimulated Raman Scattering in Pb(MoO4)1−x(WO4)x with x = 0, 0.5, 0.8 and 1.0 with Combined Frequency Shifts on High- and Low-Frequency Raman Modes under Synchronous Picosecond Laser Pumping
by Milan Frank, Sergei N. Smetanin, Michal Jelínek, David Vyhlídal, Miron B. Kosmyna, Alexey N. Shekhovstov, Ksenia A. Gubina, Vladislav E. Shukshin, Petr G. Zverev and Václav Kubeček
Crystals 2022, 12(2), 148; https://doi.org/10.3390/cryst12020148 - 21 Jan 2022
Cited by 11 | Viewed by 3263
Abstract
Comparative characteristics of spontaneous and stimulated Raman scattering in Pb(MoO4)1−x(WO4)x single crystals (x = 0, 0.5, 0.8, and 1.0) including both the high (ν1) and low (ν2) frequency internal anionic group vibrations [...] Read more.
Comparative characteristics of spontaneous and stimulated Raman scattering in Pb(MoO4)1−x(WO4)x single crystals (x = 0, 0.5, 0.8, and 1.0) including both the high (ν1) and low (ν2) frequency internal anionic group vibrations have been investigated. It was found that among these crystals, Pb(MoO4)0.2(WO4)0.8 is the most suitable for multi-wavelength Raman laser in several Raman modes simultaneously. This is caused by the optimal relative Mo/W content corresponding to the most efficient coherent combination of the (MoO4)2− and (WO4)2− vibrations enhancing the output radiation characteristics of the synchronously pumped Pb(MoO4)0.2(WO4)0.8 Raman laser. Oscillation of up to twelve, closely spaced SRS components in a range of 1128–1360 nm and the strongest pulse shortening down to 1.16 ps in comparison with not only PbMoO4 and PbWO4 but also with all the earlier investigated nominally pure scheelite-like tungstate and molybdate crystals has been achieved. Full article
(This article belongs to the Special Issue Raman Scattering in Optical Crystals)
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14 pages, 7722 KB  
Article
Effects of an Electric Field on the Conformational Transition of the Protein: Pulsed and Oscillating Electric Fields with Different Frequencies
by Qun Zhang, Dongqing Shao, Peng Xu and Zhouting Jiang
Polymers 2022, 14(1), 123; https://doi.org/10.3390/polym14010123 - 30 Dec 2021
Cited by 37 | Viewed by 4785
Abstract
The effect of pulsed and oscillating electric fields with different frequencies on the conformational properties of all-α proteins was investigated by molecular dynamics simulations. The root mean square deviation, the root mean square fluctuation, the dipole moment distribution, and the secondary structure analysis [...] Read more.
The effect of pulsed and oscillating electric fields with different frequencies on the conformational properties of all-α proteins was investigated by molecular dynamics simulations. The root mean square deviation, the root mean square fluctuation, the dipole moment distribution, and the secondary structure analysis were used to assess the protein samples’ structural characteristics. In the simulation, we found that the higher frequency of the electric field influences the rapid response to the secondary structural transitions. However, the conformational changes measured by RMSD are diminished by applying the electrical field with a higher frequency. During the dipole moment analysis, we found that the magnitude and frequency of the dipole moment was directly related to the strength and frequency of the external electric field. In terms of the type of electric fields, we found that the average values of RMSD and RMSF of whole molecular protein are larger when the protein is exposed in the pulsed electric field. Concerning the typical sample 1BBL, the secondary structure analysis showed that two alpha-helix segments both transit to turns or random coils almost simultaneously when it is exposed in a pulsed electric field. Meanwhile, two segments present the different characteristic times when the transition occurs in the condition of an oscillating electric field. This study also demonstrated that the protein with fewer charged residues or more residues in forming α-helical structures display the higher conformational stability. These conclusions, achieved using MD simulations, provide a theoretical understanding of the effect of the frequency and expression form of external electric fields on the conformational changes of the all-α proteins with charged residues and the guidance for anticipative applications. Full article
(This article belongs to the Special Issue Design and Modification of Bio-Based Polymers, Blends and Composites)
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20 pages, 4363 KB  
Article
On the Effect of CO2 on Seismic and Ultrasonic Properties: A Novel Shale Experiment
by Stian Rørheim, Mohammad Hossain Bhuiyan, Andreas Bauer and Pierre Rolf Cerasi
Energies 2021, 14(16), 5007; https://doi.org/10.3390/en14165007 - 15 Aug 2021
Cited by 10 | Viewed by 3279
Abstract
Carbon capture and storage (CCS) by geological sequestration comprises a permeable formation (reservoir) for CO2 storage topped by an impermeable formation (caprock). Time-lapse (4D) seismic is used to map CO2 movement in the subsurface: CO2 migration into the caprock might [...] Read more.
Carbon capture and storage (CCS) by geological sequestration comprises a permeable formation (reservoir) for CO2 storage topped by an impermeable formation (caprock). Time-lapse (4D) seismic is used to map CO2 movement in the subsurface: CO2 migration into the caprock might change its properties and thus impact its integrity. Simultaneous forced-oscillation and pulse-transmission measurements are combined to quantify Young’s modulus and Poisson’s ratio as well as P- and S-wave velocity changes in the absence and in the presence of CO2 at constant seismic and ultrasonic frequencies. This combination is the laboratory proxy to 4D seismic because rock properties are monitored over time. It also improves the understanding of frequency-dependent (dispersive) properties needed for comparing in-situ and laboratory measurements. To verify our method, Draupne Shale is monitored during three consecutive fluid exposure phases. This shale appears to be resilient to CO2 exposure as its integrity is neither compromised by notable Young’s modulus and Poisson’s ratio nor P- and S-wave velocity changes. No significant changes in Young’s modulus and Poisson’s ratio seismic dispersion are observed. This absence of notable changes in rock properties is attributed to Draupne being a calcite-poor shale resilient to acidic CO2-bearing brine that may be a suitable candidate for CCS. Full article
(This article belongs to the Special Issue Geomechanics Applied to Carbon Capture and Storage)
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