Search Results (11)

The wetlands of Sardinia (Italy) supply food and shelter for many waterbird species that migrate along the central–eastern Mediterranean bird flyway. Despite many different policies and laws (the Birds and Habitats Directives, the European Water Framework Directive, and the Ramsar Convention), the Sardinian wetlands are seriously threatened by human activities and climate change, which in turn menace the associated avifauna. In this study, we (a) inventoried (four sampling dates) the avian metacommunity of the largest coastal wetlands in Sardinia during the crucial period of the year for the avifauna (August–September), (b) explored the spatiotemporal dynamics in bird species assemblage, and (c) used results to refine planning for bird habitat management and bird diversity conservation. We recorded 60 bird species, of which 54 were migratory and 21 belonged to Annex I of the Birds Directive. During August–September, (a) α, β, and γ avian diversity showed no significant temporal trends, (b) the contributions of space (wetlands) and time (dates of sampling) in determining the presence/absence of the waterbird species were comparable, (c) wetlands formed three statistically significant clusters with regard to the species richness, (d) a significant increase in the number of the species belonging to the “mixed” migration guild, and “divers from the surface” foraging guild, occurred, (e) there was a statistically significant chronological succession of the occurrence of waterbird species, (f) twenty-five species made use of the Sardinian wetlands all summer long, while ten further species were present in three sampling dates out of four, (g) the spatial distributions of the waterbird species in the Sardinian wetlands were significantly different between the sampling dates, (h) the Little Egret, the Grey Heron, and the Greater Flamingo were primarily responsible for the observed difference in the spatial distributions of species between the sampling dates, (i) Is Brebeis, Pilo, and S. Giovanni were the wetlands that changed their species composition the most during the studied period, (j) twenty-two waterbird species resulted at high priority for conservation, and thirteen species at medium priority. Based on these results, we have proposed new strategies for the conservation of the waterbird species of the Sardinian wetlands during the post-breeding migration period. Full article

The Sicilian wetlands (Italy) are seriously threatened by human activities and ongoing climate change. The loss of these wetlands as migratory stepping stones could severely hamper the migratory flow of many bird species along the central Mediterranean. Targeted actions for the conservation of the avifauna require thorough knowledge of the utilization that waterbirds make of these habitats. Aiming to inform planning for more satisfactory bird habitat management and bird diversity preservation along the Mediterranean migratory bird flyway, in this study, we inventoried the avian metacommunity of the coastal wetlands in Sicily during the most critical period of the year (July–September) and used a multilevel analytical framework to explore the spatiotemporal dynamics in bird species assemblages. We recorded 73 bird species, of which almost 90% were migratory and 30 belonged to Annex I of the Birds Directive. At the metacommunity level, we found that all the biodiversity metrics were low in July and approximately doubled in the successive sampling sessions (August–September), where they showed little if any change. At the community level, we detected two main clusters of wetlands with regard to species richness, of which one (wetlands Baronello, Gela, Gornalunga, and Roveto) was characterized by higher levels of species richness in nearly all the sampling dates. The pattern of species richness in the Sicilian wetlands was most similar between the first and second half of August, while July was very dissimilar from all the other sampling dates. At the guild level, we found a significant increase during July–September in the number of the species belonging to the “Mediterranean” migration guild and the “divers from the surface” and “surface feeders” foraging guilds. At the species level, we detected a significant temporal sequence of the occurrence of waterbird species: two species were only early dwellers in July, ten species were only late dwellers in September, and twenty-six species made use of the Sicilian wetlands all summer long. The spatial distribution of the waterbird species differed significantly between any pair of sampling dates. Overall, the Little Grebe, the Spotted Redshank, and the Little Tern were the bird species with the highest site infidelity; by contrast, the Black Stork, the Broad-billed Sandpiper, the European Golden Plover, the Common Shelduck, and the Black-necked Grebe changed their spatial distribution among wetlands the least during July–September. Our study allowed us to detect (1) the wetlands and (2) the waterbird species to which the priority for conservation should be assigned, as well as (3) the exact time span during July–September when conservation measures should be mandatory, and not only advisable. These results provide a broader insight of the space–time patterns in bird species assembly in the coastal wetlands of Sicily during the critical summer period. Full article

Cynometra iripa Kostel. is a Fabaceae species of mangrove used in traditional Ayurvedic medicine for treating inflammatory conditions. The present study aims to establish monographic botanical and chemical quality criteria for C. iripa leaf and bark as herbal substances and to evaluate their in vitro antioxidant potential. Macroscopic and microscopic qualitative and quantitative analyses, chemical LC-UV/DAD-ESI/MS profiling, and the quantification of key chemical classes were performed. Antioxidant activity was evaluated by DPPH and FRAP assays. Macroscopically, the leaf is asymmetrical with an emarginated apex and cuneate base. Microscopically, it shows features such as two-layered adaxial palisade parenchyma, vascular bundles surrounded by 3–6 layers of sclerenchyma, prismatic calcium oxalate crystals (5.89 ± 1.32 μm) along the fibers, paracytic stomata only on the abaxial epidermis (stomatal index–20.15), and non-glandular trichomes only on petiolules. The microscopic features of the bark include a broad cortex with large lignified sclereids, prismatic calcium oxalate crystals (8.24 ± 1.57 μm), and secondary phloem with distinct 2–5 seriated medullary rays without crystals. Chemical profile analysis revealed that phenolic derivatives, mainly condensed tannins and flavonoids, are the main classes identified. A total of 22 marker compounds were tentatively identified in both plant parts. The major compounds identified in the leaf were quercetin-3-O-glucoside and taxifolin pentoside and in the bark were B-type dimeric proanthocyanidins and taxifolin 3-O-rhamnoside. The total phenolics content was higher in the leaf (1521 ± 4.71 mg GAE/g dry weight), while the total flavonoids and condensed tannins content were higher in the bark (82 ± 0.58 mg CE/g and 1021 ± 5.51 mg CCE/g dry weight, respectively). A total of 70% of the hydroethanolic extracts of leaf and bark showed higher antioxidant activity than the ascorbic acid and concentration-dependent scavenging activity in the DPPH assay (IC₅₀ 23.95 ± 0.93 and 23.63 ± 1.37 µg/mL, respectively). A positive and statistically significant (p < 0.05) correlation between the phenol content and antioxidant activity was found. The results obtained will provide important clues for the quality control criteria of C. iripa leaf and bark, as well as for the knowledge of their pharmacological potential as possible anti-inflammatory agents with antioxidant activity. Full article

In this study, the integration of useful statistical methods from different disciplines for analyzing the forest community of the Lienhuachih area of central Taiwan was attempted. We employed a seriated heat map to confirm the presence of multiple community patterns in the area and used the gap statistics and a clustplot to confirm the number and structure of the patterns, respectively. A minimum spanning tree was used to display a succession series among the quadrats, and Renyi diversity was used to indicate the species composition of these patterns. The results confirmed the existence of six patterns with different biodiversity structures in which pattern C was the succession prototype of the local community patterns. Next, we used the patterns as nodes of a social network to perform bipartite network analysis. The results showed that a community network consisted of 108 taxa and six syntaxa. The syntaxa were highly vulnerable to extinction; therefore, the optimal conservation strategy for local species would be to first protect the syntaxa. The random forest method and bipartite modularity were used to analyze the dominant characteristic species of the six syntaxa. The results showed that these two methods are useful for detecting characteristic species of the syntaxa. Therefore, this study proposed a new nomenclature system, namely the Mathematic Code of Syntaxonomic Nomenclature, to support the results of numerical vegetation analysis. Finally, the potential for an apparently competitive network was examined, the role of an apparently competitive network in the local structuring community was explored, and six new associations in the Lienhuachih area were described. Full article

Background: The major limitation to the Ross operation is a progressive autograft dilation, possibly leading to reoperations. A murine model was created to evaluate pulmonary artery graft (PAG) adaptation to pressure overload. Methods: Lewis rats (n = 17) underwent heterotopic surgical implantation of a PAG, harvested from syngeneic animals (n = 17). A group of sham animals (n = 7) was used as a control. Seriated ultrasound studies of the PAG were performed. Animals were sacrificed at 1 week (n = 5) or 2 months (n = 15) and the PAG underwent mechanical and histopathological analyses. Results: Echography showed an initial increase in diameter (p < 0.001) and a decrease in peak systolic velocity (PSV). Subsequently, despite no change in diameter, an increase in PSV was observed (p < 0.01). After 1 week, the stiffness of the PAG and the aorta were similar, while at 2 months, the PAG appeared more rigid (p < 0.05). PAG’s histological analysis at 2 months revealed intimal hyperplasia development. The tunica media showed focal thinning of the elastic lamellae and normally distributed smooth muscle cells. Conclusions: We demonstrated a stiffening of the PAG wall after its implantation in systemic position; the development of intimal hyperplasia and the thinning of the elastic lamellae could be the possible underlying mechanism. Full article

We reconstructed the seismotectonic setting of the area comprising the northeastern Friuli Plain and the Julian pre-Alpine border (NE Italy) by integrating geological and seismological data. The study area represents the junction between the SSE-verging polyphase thrust-front of the south-Alpine Chain and the NW–SE-trending strike-slip faults of the eastern Friuli–western Slovenia domain. Following a multidisciplinary approach, the 3D geometry of the Susans–Tricesimo thrust system was reconstructed through the elaboration of four geological cross sections derived from the interpretation of ENI industrial seismic lines. In a second step, the seismogenic volume of the central-eastern Friuli area was investigated through hypocentral distribution analysis: the seismic events of the latest 50 years (1976–1977 and 1978–2019 time intervals) were plotted on four NE-SW-oriented seriated sections together with the fault plane’s geometry. Through this procedure, we were able to investigate the relationship between the NW-SE-striking high-angle faults, which characterize the northern Julian pre-Alps, and the WSW-verging medium-angle reverse fronts located at the piedmont of the Friuli plain, which experienced NW-SE- to NNW-SSE-oriented compression starting at least from the Pliocene. In detail, we examined the involvement of these structures during the seismic sequences of May and September 1976, in terms of activation and/or interaction. The resulting seismotectonic model highlights the interplay between transpressive/strike-slip and reverse planes. In particular, this study suggests that Predjama and Maniaglia transpressive faults strongly control the stress release and likely played a fundamental role both during the 6 May (Mw 6.5) and 15 September (Mw 6.0) Friuli earthquakes. Full article

Background: The most common conservative treatment for Adolescent Idiopathic Scoliosis (AIS) is bracing. However, several papers questioned the effectiveness of bracing for curves between 40° and 50° Cobb: the effectiveness in preventing curve progression could be as low as 35%. Seriate casting is considered a standard approach in early onset scoliosis; however, in the setting of AIS, cast treatment is seldom utilized, with only few studies reporting on its effectiveness. Aim of the study: The main aim of the study is to determine whether a seriate casting with Risser casts associated with bracing is more effective in preventing curve progression than bracing alone in curves larger than 40°. Furthermore, the secondary endpoints were: (1) is there a difference in effectiveness of casting between Thoracic (T) and Thoracolumbar/Lumbar (TL/L) curves? (2) Does the ‘in cast’ correction predicts the treatment outcome? (3) What is the effect on thoracic kyphosis of casting? Methods: This is a retrospective monocentric case–control study; through an Institutional Database search we identified all the patients treated at our institution between 1 January 2017 and 31 December 2020, with a diagnosis of AIS, Risser grade between 0 and 4 at the beginning of the treatment, at least one curve above 40° Cobb and treatment with either seriate Risser casting and bracing (Study Group, SG) or bracing alone (Control Group, CG). Standing full spine X-rays in AP and LL are obtained before and after the cast treatment; only AP standing full spine X-rays ‘in-cast’ are obtained for each cast made. Patients were stratified according to the curve behavior at the end of treatment (Risser 5): progression was defined as ≥6° increase in the curve magnitude or fusion needed; stabilization is defined as a change in curve by ±5°; and improvement was defined as ≥6° reduction in the curve. Results: For the final analysis, 55 compliant patients (12 M, 43 F, mean age 13.5 ± 1.6) were included in the SG and 27 (4 M, 23 F, mean age 13.6 ± 1.6) in the CG. Eight (14.5%) patients in the SG failed the conservative treatment while 14 (51.3%) failed in the CG. Consequently, the Relative Risk for progression in the Efficacy Analysis was 1.8 (95% CI 1, 3–2.6, p = 0.001), and the Number Needed to Treat was 2,4. No significant difference was found between the T and TL/L curves concerning the ‘progressive’ endpoint (z-score 0.263, p = 0.79). The mean percentage of ‘in cast’ curve reduction was 40.1 ± 15.2%; no significant correlation was found between the percentage of correction and the outcome (Spearman Correlation Coefficient 0.18). Finally, no significant differences between baseline and end of FU TK were found (32° ± 16.2 vs. 29.6 ± 15.8, p = ns). Discussion: Seriate Risser casting for AIS with larger curves (>40° Cobb) is effective in reducing curve progression when compared with full time bracing alone in treatment compliant patients. The treatment is equally effective in controlling T and TL/L curves; furthermore, a slight but non-significant decrease in TK was observed in patients treated with casting. This type of treatment should be considered for AIS patients who present with large curves to potentially reduce the percentage of surgical cases. Short Abstract: The aim of the study is to determine whether seriate Risser casting associated with bracing is more effective in preventing curve progression than bracing alone in curves larger than 40°. This is a retrospective monocentric case–control study; we identified all the patients treated at our institution with a diagnosis of AIS, Risser grade 0–4 at the beginning of the treatment, at least one curve above 40° Cobb (35° if treated with bracing alone) and treatment with either seriate Risser casting and bracing (Study Group, SG) or bracing alone (Control Group, CG). Fifty-five patients (12 M, 43 F, mean age 13.5 ± 1.6) were included in the SG and 30 (5 M, 25 F, mean age 13.9 ± 1.7) in the CG. Eight (14,5%) patients in the SG failed the conservative treatment while fifteen (50%) failed in the CG. Consequently, the Relative Risk for progression in the Efficacy Analysis was 1.8 (95% CI 1.3–2.6, p = 0.001), and the Number Needed to Treat was 2,4. Seriate Risser casting for AIS with larger curves (>40°) is effective in reducing curve progression when compared with full time bracing alone. This type of treatment should be considered for AIS patients who present with large curves. Full article

Improvements in Botulinum toxin type-A (BoNT-A) aesthetic treatments have been jeopardized by the simplistic statement: “BoNT-A treats wrinkles”. BoNT-A monotherapy relating to wrinkles is, at least, questionable. The BoNT-A mechanism of action is presynaptic cholinergic nerve terminals blockage, causing paralysis and subsequent muscle atrophy. Understanding the real BoNT-A mechanism of action clarifies misconceptions that impact the way scientific productions on the subject are designed, the way aesthetics treatments are proposed, and how limited the results are when the focus is only on wrinkle softening. We designed a systematic review on BoNT-A and muscle atrophy that could enlighten new approaches for aesthetics purposes. A systematic review, targeting articles investigating BoNT-A injection and its correlation to muscle atrophy in animals or humans, filtered 30 publications released before 15 May 2020 in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Histologic analysis and histochemistry showed muscle atrophy with fibrosis, necrosis, and an increase in the number of perimysial fat cells in animal and human models; this was also confirmed by imaging studies. A significant muscle balance reduction of 18% to 60% after single or seriated BoNT-A injections were observed in 9 out of 10 animal studies. Genetic alterations related to muscle atrophy were analyzed by five studies and showed how much impact a single BoNT-A injection can cause on a molecular basis. Seriated or single BoNT-A muscle injections can cause real muscle atrophy on a short or long-term basis, in animal models and in humans. Theoretically, muscular architecture reprogramming is a possible new approach in aesthetics. Full article

Taxol drugs can be extracted from various species of the taxaceae family. It is an alkaloid (metabolic product) used for the treatment of various types of cancer. Since taxol is a metabolic product, multiple aspects such as edaphic, biochemical, topographic factors need to be assessed in determining the variation in Taxol Content (TC). In this study, both sensor-based hyperspectral reflectance data and absorption-based indices were tested together for the development of an advanced statistical unfolding approach to understand the influencing factors for TC in high altitude Himalayan region. Seriation analysis based on permutation matrix was applied with complete linkage and a multi-fragment heuristic scaling rule along with the common techniques such as Principal Component Analysis (PCA) and correlation to understand the relationship of TC with various factors. This study also tested the newly developed taxol indices to rule out the possibility of overlapping of TC determining bands with the foliar pigment’s wavelengths in the visible region. The result implies that T. wallichiana with a high TC is found more in its natural habitat of deep forest, relating it indirectly to elevation in the case of the montane ecosystem. Taxol is the most varying parameter among the measured variables, followed by hyperspectral Taxol content (TC) indices such as TC 2, TC 5, and carotenoids, which suggests that the indices are well versed to capture variations in TC with elevation. Full article

Recent developments in the availability of very high-resolution satellite imagery through platforms like GoogleEarth (Google, Santa Clara County, CA, USA) and Bing Maps (Microsoft, Redmond, WA, USA) have greatly opened up the possibilities of their use by researchers. This paper focusses on the exclusive use of free remote sensing data by the Western Harra Survey (WHS), an archaeological project investigating the arid “Black Desert” of north-eastern Jordan, a largely impenetrable landscape densely strewn with basalt blocks. The systematic analysis of such data by conducting a holistic satellite survey prior to the commencement of fieldwork allowed for the precise planning of ground surveys, with advanced knowledge of which sites were vehicle-accessible and how to efficiently visit a stratified sample of different site types. By subsequently correlating the obtained ground data with this analysis, it was possible to create a typological seriation of the site forms known as “wheels”, determine that at least two-thirds of sites are within 500 m of valleys or mudflats (highlighting these features’ roles as access routes and resource clusters) and identify numerous anthropogenic paths cleared through the basalt for site access and long-distance travel. These results offer new insights into this underrepresented region and allow for supra-regional comparisons with better investigated areas by a method that is rapid and cost-effective. Full article

The seriated extracts of petroleum ether (PE-E), dichloromethane (DCM-E) and methanol extracts (MeOH-E) from the aerial parts of the native South American plant Tetraglochin ameghinoi (Rosaceae), were evaluated regarding their antioxidant and antibacterial activities. The antioxidant properties were evaluated by free radical scavenging methods (DPPH and TEAC), ferric-reducing antioxidant power (FRAP) and lipoperoxidation in erythrocytes (LP), while the antibacterial activity was performed against Gram-positive and Gram-negative bacteria according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The chemical and biological analyses of this plant are very important since this bush is currently used in traditional medicine as a cholagogue and digestive. The polar MeOH-E showed the highest antioxidant activities (17.70 µg/mL in the DPPH assay, 381.43 ± 22.38 mM TE/g extract in the FRAP assay, 387.76 ± 91.93 mg TE/g extract in the TEAC assay and 93.23 + 6.77% in the LP assay) and it was selected for chromatographic isolation of its components. These components were found to be four acetophenones, including the new phloracetophenone glucoside: 4′,6′,-dihydroxy-2′-O-(6″-acetyl)-β-d-glucopyranosylacetophenone or IUPAC name: (6-(2-acetyl-3,5-dihydroxyphenoxy)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)methyl acetate, whose structure was elucidated by NMR and MS methods. In addition, twenty-six compounds, including five of these acetophenone derivatives, two sugars, six flavonoids, eleven phenolic acids and two triterpenes, were identified based on UHPLC-OT-MS and PDA analysis on the MeOH-E. The results support the medicinal use of the plant. Full article