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Keywords = royal patronage

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20 pages, 2681 KiB  
Article
Molecular Characterization of CnHd3a and Spatial Expression of Its Alternative Splicing Forms Associated with Flowering Transition and Flower Development in Coconut Palm (Cocos nucifera L.)
by Pariya Maneeprasert, Siriwan Thaisakun, Theerachai Thanananta, Narumol Thanananta, Noppamart Lokkamlue and Chareerat Mongkolsiriwatana
Genes 2025, 16(6), 718; https://doi.org/10.3390/genes16060718 - 18 Jun 2025
Viewed by 604
Abstract
Background: The flowering transition is a critical process determining the onset of reproductive development and fruit production. The molecular mechanisms underlying this process in coconuts are poorly understood; however, recent studies have identified CnHd3a as a potential regulator of the floral transition in [...] Read more.
Background: The flowering transition is a critical process determining the onset of reproductive development and fruit production. The molecular mechanisms underlying this process in coconuts are poorly understood; however, recent studies have identified CnHd3a as a potential regulator of the floral transition in coconuts. Methods: In this study, we characterized the molecular structure of CnHd3a and analyzed its alternative splicing forms in tall and dwarf varieties of coconut palms during the flowering transition. We used qRT-PCR to measure the expression levels of CnHd3a at different developmental stages. Results: CnHd3a was expressed in leaves and the shoot apical meristem (SAM) during the flowering transition in both coconut varieties and flower tissues during flower development. Interestingly, the expression levels of complex isoforms of CnHd3a were higher in the leaves of dwarf coconuts than in those of tall coconuts, suggesting their involvement in shortening the vegetative growth phase of dwarf coconuts. The gene structure of CnHd3a was found to be conserved across different plant species, indicating the evolutionary conservation of the floral transition process. Conclusions: Our findings provide insight into the molecular mechanisms underlying the floral transition and flower development processes in coconut palm. The tissue-specific expression patterns of CnHd3a isoforms show their potential roles in growth and development. Further investigations focusing on the functional characterization of CnHd3a isoforms will have practical implications for coconut breeding and cultivation strategies. Full article
(This article belongs to the Section Plant Genetics and Genomics)
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27 pages, 13168 KiB  
Article
Framing the Calendar of the Sacramentary of Messina (BNE, Ms. 52): Patronage and Byzantine Topics in Late 12th-Century Sicilian Art
by Carles Sánchez Márquez
Arts 2025, 14(2), 32; https://doi.org/10.3390/arts14020032 - 20 Mar 2025
Viewed by 913
Abstract
For the Norman kings of Sicily and the ecclesiastical authorities who ruled their dioceses, Byzantine art served as both a symbol of luxury and a model of prestige. Similarly to the mosaics of Palermo, Monreale, and Cefalú, as well as textiles and goldsmithing, [...] Read more.
For the Norman kings of Sicily and the ecclesiastical authorities who ruled their dioceses, Byzantine art served as both a symbol of luxury and a model of prestige. Similarly to the mosaics of Palermo, Monreale, and Cefalú, as well as textiles and goldsmithing, the manuscripts preserved in the National Library of Madrid stand as prime examples of the fascination that the dignitaries of the Kingdom of Sicily had for Byzantine esthetics. Among these manuscripts, the Sacramentary of Messina (Madrid, BNE Ms. 52) is perhaps the most striking. This Latin sacramentary, comprising 303 folios, features illuminated initials, a calendar with depictions of classical topics, such as the Spinario and a compelling depiction of August inspired by the Byzantine Koimesis, the months and zodiac, and two full-page illustrations depicting the Virgin Glykophilousa, the Crucifixion, and the Deesis. This study has a dual focus. First, it aims to analyze the iconographic peculiarities of the monthly images in this Latin calendar. Second, it seeks to provide new insights into the manuscript’s patronage and its place of origin. In this context, one of the most striking and significant aspects of the sacramentary’s iconography is the prominent role of the Virgin, a theme that will also be examined in this study. Archbishop Richard Palmer emerges as the leading candidate to have been the driving force in the patronage of the manuscript to the Royal scriptoria of Palermo. Full article
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16 pages, 313 KiB  
Article
Overcoming the Patronage System: Propaganda Fide and Its Portuguese Patronage Documents on China in the Early Modern Period
by Rui Zhang
Religions 2024, 15(8), 959; https://doi.org/10.3390/rel15080959 - 7 Aug 2024
Cited by 2 | Viewed by 1868
Abstract
From its foundation in 1622, the Congregation de Propaganda Fide held supreme jurisdiction over all missionary activities of the Catholic Church at the global level, with authority coming directly from the pope. Its main goals were the expansion of the Catholic faith, the [...] Read more.
From its foundation in 1622, the Congregation de Propaganda Fide held supreme jurisdiction over all missionary activities of the Catholic Church at the global level, with authority coming directly from the pope. Its main goals were the expansion of the Catholic faith, the reaffirmation of papal spiritual primacy, and the promotion of Indigenous clergy throughout the world, which put Propaganda Fide in direct competition with secular rulers who also wanted to control missionary activities in their overseas territories. This article will examine one such conflict, namely that regarding Portugal and its royal patronage system in China. The Historical Archive of Propaganda Fide in the Vatican City holds extensive material concerning Portuguese patronage and China, which has remained relatively unknown and understudied. Through an examination of several of the most significant documents, such as the formal terms sent by the Portuguese court to the Holy See, the response of Propaganda Fide, and the final declaration of the Holy See, which would become the cornerstone for all future decisions over the patronage system, it is suggested that these negotiations about China are in fact central to a wider shift in global Catholic policy and politics in the field of missionary activity. Full article
15 pages, 509 KiB  
Article
Effects of Substituting Cassava Pulp with Broken Rice and Cassava Chips in Crossbred Holstein Diets: Rumen Fermentation, Enteric Methane Emission, and Energy Utilization
by Jiraporn Kabsuk, Jenwit Nusri-un, Bhoowadol Binsulong, Thidarat Gunha and Kritapon Sommart
Animals 2024, 14(15), 2257; https://doi.org/10.3390/ani14152257 - 3 Aug 2024
Viewed by 1211
Abstract
This study evaluates the effects of substituting cassava pulp with broken rice and cassava chips in the total mixed ration silage diets of beef cattle on feed composition, ensiling quality, digestibility, and energy utilization. Fifteen Holstein Thai native crossbred (89% Bos taurus × [...] Read more.
This study evaluates the effects of substituting cassava pulp with broken rice and cassava chips in the total mixed ration silage diets of beef cattle on feed composition, ensiling quality, digestibility, and energy utilization. Fifteen Holstein Thai native crossbred (89% Bos taurus × 11% Bos indicus) steers in the fattening phase, with an average age of 2.5 ± 0.1 years and an initial body weight of 603.7 ± 14.3 kg, were used in the energy balance trial. Using a randomized complete block design with five replications, the steers received one of three treatments. The three dietary treatments included substituting cassava pulp with cassava chips and broken rice on a dry matter basis with ratios of 50:0:0, 30:20:0, or 10:20:20. The results show that broken rice is a superior nutrient source and provides greater energy balance (p < 0.01). Despite the cost implications, substituting cassava pulp and chips positively impacts the ensilage pH and reduces the acetic acid concentration (p < 0.01). There was an increase in the lactic acid bacteria count (p < 0.05) and a reduction in the rumen ammonia, propionate, and butyrate concentrations (p < 0.05) without adverse effects (p > 0.05) on digestibility, blood metabolites, or enteric methane emissions. These findings suggest that broken rice is a promising alternative grain-rich ruminant feed. Future research should explore on-farm long-term feeding and economic evaluations to provide a more comprehensive understanding of the practical implications. Full article
(This article belongs to the Section Animal Nutrition)
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13 pages, 346 KiB  
Article
Influence of Environmental Risk Exposure on the Determinants of COVID-19 Booster Vaccination in an Urban Thai Population
by Weerawat Ounsaneha, Orapin Laosee and Cheerawit Rattanapan
Int. J. Environ. Res. Public Health 2024, 21(6), 745; https://doi.org/10.3390/ijerph21060745 - 6 Jun 2024
Cited by 2 | Viewed by 1698
Abstract
This study aimed to identify the influence of environmental risk exposure levels on the predictive factors of COVID-19 booster dose vaccination in an urban Thai population in the post-pandemic era. Six study locations, including the three provinces with the highest environmental risk levels [...] Read more.
This study aimed to identify the influence of environmental risk exposure levels on the predictive factors of COVID-19 booster dose vaccination in an urban Thai population in the post-pandemic era. Six study locations, including the three provinces with the highest environmental risk levels and the three provinces with the lowest environmental risk levels, were selected by calculating the environmental risk exposure indexes. Participants from the capital district of each province were chosen via the simple random sampling technique and interviewed using a structured questionnaire. A total of 1315 individuals were included in a sample in this study, and the best predictors of booster dose vaccination were determined using multiple regression analysis. The results showed that a high level of environmental risk exposure occurred in the provinces with a high number of total days exceeding the limits set for PM10 and high rates of mortality for lung cancer. The number of COVID-19 booster vaccinations given amount to 43.4% of the population during the post-COVID-19 pandemic period. Our multivariate analysis indicated that individuals in the working age group (≥25 years old); those with higher education (diploma degree and above); full-time employment (government and private sectors); those with high monthly incomes (≥USD144.1); and those in areas with the lowest risk level of environmental exposure significantly contributed to the number of booster dose vaccinations given during the post-pandemic period. To summarize, the rate of COVID-19 booster dose vaccination acceptance in Thailand was influenced by socio-economic factors with environmental concerns. These findings improve our understating of both the global pandemic and how environmental exposure affects behavioral change patterns and could improve the effectiveness of post-pandemic management. Full article
(This article belongs to the Special Issue Pandemic Preparedness: Lessons Learned from COVID-19)
22 pages, 6455 KiB  
Article
Ritual, Daoist Temple, and Geography: Spatial Interpretation of Wang Lingguan’s Belief
by Zhaoquan He and Xiaorong Meng
Religions 2024, 15(3), 305; https://doi.org/10.3390/rel15030305 - 29 Feb 2024
Viewed by 4232
Abstract
Wang Lingguan is a significant deity in Chinese Daoist beliefs and folk worship. His belief’s formation and proliferation are rooted in specific spatial contexts. This paper introduces a spatial perspective to provide a fresh interpretation of Wang Lingguan’s belief, examining it through the [...] Read more.
Wang Lingguan is a significant deity in Chinese Daoist beliefs and folk worship. His belief’s formation and proliferation are rooted in specific spatial contexts. This paper introduces a spatial perspective to provide a fresh interpretation of Wang Lingguan’s belief, examining it through the lenses of ritual, temple, and geography. In Daoist rituals that bridged sacred and secular spaces, Wang Lingguan emerged as Sa Shoujian’s protector, manifesting his divine power to devotees. For the purposes of ritual simplification and spatial solidification, believers constructed Daoist Temples as emblems of sacredness and reimagined Wang Lingguan as the protector of these temples in their design. The active involvement of the Ming royal family in building Daoist Temples significantly contributed to establishing regional belief centers for Wang Lingguan. During the Qing Dynasty, although Wang Lingguan’s royal patronage waned, his belief spread across most of China, becoming more localized and secularized. The dynamic interplay of ritual, temple, and geographical factors illuminates the establishment, dissemination, and evolution of Wang Lingguan’s belief throughout China. Full article
(This article belongs to the Special Issue Space for Worship in East Asia)
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20 pages, 667 KiB  
Article
The Confidence of and Concern about Using Mobile Banking among Generation Z: A Case of the Post COVID-19 Situation in Thailand
by Wischaya Silanoi, Phaninee Naruetharadhol and Khwanjira Ponsree
Soc. Sci. 2023, 12(4), 198; https://doi.org/10.3390/socsci12040198 - 27 Mar 2023
Cited by 11 | Viewed by 6048
Abstract
This research paper focuses on mobile banking acceptance among Generation Z users by combining the technology acceptance model (TAM) and the unified theory of acceptance and use of technology (UTAUT) theory, as well as extending the theories with perceived trust and risk. During [...] Read more.
This research paper focuses on mobile banking acceptance among Generation Z users by combining the technology acceptance model (TAM) and the unified theory of acceptance and use of technology (UTAUT) theory, as well as extending the theories with perceived trust and risk. During the COVID-19 pandemic, the online questionnaire survey was distributed using Google Forms, and the sample group was Thai Generation Z who were aged between 18 and 25 years old. The research objectives aim to (i) investigate the crucial effects that potentially affect user intention and actual usage of mobile banking, (ii) identify the most influential factor impacting users’ intention and behavior, (iii) further study the trust and risk perception of Generation Z users on mobile-banking intention and actual usage, (iv) discuss the findings with the antecedent studies, and (v) contribute the research findings both theoretically and practically. The proposed constructs include perceived usefulness, perceived ease of use, social influence, facilitating conditions, perceived trust, perceived risk, behavioral intention, and actual usage. There are fourteen proposed hypotheses to be tested. Based on the outcomes and the standardized coefficient beta, perceived usefulness (β = 0.518) was the strongest factor determining Generation Z’s behavioral intention, while perceived ease of use (β = 0.809) impacting perceived usefulness demonstrated the strongest relationship among all of the hypotheses. Full article
(This article belongs to the Section Social Economics)
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13 pages, 1183 KiB  
Article
Preventive Behaviors and Influencing Factors among Thai Residents in Endemic Areas during the Highest Epidemic Peak of the COVID-19 Outbreak
by Weerawat Ounsaneha, Orapin Laosee, Thunwadee Tachapattaworakul Suksaroj and Cheerawit Rattanapan
Int. J. Environ. Res. Public Health 2023, 20(3), 2525; https://doi.org/10.3390/ijerph20032525 - 31 Jan 2023
Cited by 5 | Viewed by 2733
Abstract
This research aims to investigate COVID-19 preventive behavior and influencing factors among Thai residents during the highest epidemic peak of COVID-19. Nine hundred and forty-six residents in five districts with high COVID-19 infection cases in Thailand were systematically included in this cross-sectional survey. [...] Read more.
This research aims to investigate COVID-19 preventive behavior and influencing factors among Thai residents during the highest epidemic peak of COVID-19. Nine hundred and forty-six residents in five districts with high COVID-19 infection cases in Thailand were systematically included in this cross-sectional survey. The results showed that 87.2% and 65.2% of the residents had a high level of general knowledge and preventive measures, respectively. As to COVID-19 attitudes, poor levels of attitude among Thai residents were found in risk perception (53.6%) and mistrust issues (70.4%). Moreover, this study presents good preventive behavior (77.0%) among Thai residents. Multiple logistic regression showed that the influence factors of COVID-19 preventive behavior were the young age group (AOR 2.97, 95% CI 1.68–5.25), high income (AOR 1.38, 95% CI 1.03–1.86), and high level of general COVID-19 knowledge (AOR 2.21, 95% CI 1.64–2.96). The conclusion was that providing information on COVID-19 via social media was the key mechanism of policy action for increasing the level of COVID-19 preventive behavior during the highest epidemic peak in Thailand. In addition, the pandemic preparedness and response policy, with resident participation and involvement, could be recommended for the resilience of pandemic preparedness. Full article
(This article belongs to the Special Issue Health Psychology and Behaviors during COVID-19)
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15 pages, 1445 KiB  
Article
Combined Impact of Omicron Vaccination and Environmental Risk Exposure: A Thailand Case Study
by Weerawat Ounsaneha, Orapin Laosee, Thunwadee Tachapattaworakul Suksaroj and Cheerawit Rattanapan
Vaccines 2023, 11(2), 297; https://doi.org/10.3390/vaccines11020297 - 29 Jan 2023
Cited by 2 | Viewed by 2348
Abstract
This research aimed to determine the levels of COVID-19 booster dose vaccinations in Thai populations in areas with environmental risk exposure during the Omicron outbreak. Five of twenty provinces in Thailand were selected by assessing environmental risk exposure for study settings. A total [...] Read more.
This research aimed to determine the levels of COVID-19 booster dose vaccinations in Thai populations in areas with environmental risk exposure during the Omicron outbreak. Five of twenty provinces in Thailand were selected by assessing environmental risk exposure for study settings. A total of 1038 people were interviewed by a structured questionnaire. The predicting factors of COVID-19 booster dose vaccinations were analyzed by univariate and multivariate analysis. The results showed that 69.4% (95% CI 66.5–72.1) of the population was vaccinated with COVID-19 booster doses. Multiple logistics regression revealed that the female gender (AOR 1.49, 95% CI 1.11–2.00), all age groups from 38 to 60 years old, all education levels of at least secondary school, high income (AOR 1.16, 95% CI 1.15–2.24), populations having experience with COVID-19 infection (AOR 2.27, 95% CI 2.05–3.76), knowledge of vaccine (AOR 1.78, 95% CI 1.11–2.83), and trusting attitude (AOR 1.76, 95% CI 1.32–2.36) were factors among those more likely to take COVID-19 booster dose vaccinations in high-environmental-risk-exposure areas. Therefore, an effective booster dose campaign with education programs to increase attitudes toward booster vaccinations should be implemented for the resilience of COVID-19 prevention and control. Full article
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20 pages, 712 KiB  
Article
Gross National Happiness in Bhutan: Is Buddhist Constitutionalism Legitimate in the Age of Secularism? A Post-Colonial View
by Dorine Eva van Norren
Religions 2023, 14(1), 72; https://doi.org/10.3390/rel14010072 - 4 Jan 2023
Cited by 3 | Viewed by 5119
Abstract
Bhutan’s current constitution draws upon the historical dual system of religious-civil governance under the monarchy (previously Abbot-king) embodying the Mahayana Buddhist concept of Boddhisatva-leadership. Bhutan’s democracy includes an executive-military and pluralist religious custodian role for the King who can be abdicated by parliament. [...] Read more.
Bhutan’s current constitution draws upon the historical dual system of religious-civil governance under the monarchy (previously Abbot-king) embodying the Mahayana Buddhist concept of Boddhisatva-leadership. Bhutan’s democracy includes an executive-military and pluralist religious custodian role for the King who can be abdicated by parliament. It includes Gross National Happiness as spiritual core, which is non-binding law, incorporating many human rights and human values like compassion. The ban on proselytization in the secular constitution should be viewed from a geopolitical post-colonial perspective of the Christian civilization mission and India/China annexation-politics, and Asian definitions of secularism (Royal patronage of religious pluralism). Christians do experience restrictions on congregation. Hindu Nepalese-origin migrants experience(d) citizenship issues due to geopolitical context but can express religion fully. GNH-policy also has certain implementation difficulties and the GNH index indicates declining community values and spirituality in the face of modernist development. Bhutan’s constitution does not fall within the definition of theocracy. The clergy is excluded from the electoral process. The King’s authority is mainly based on moral leadership, popular uncertainty about imported democracy, and is non-absolute but larger than conventional constitutional monarchies. The constitution is more secular than Buddhist in its binding provisions and offers space for non-GNH oriented governments, also in recent practice. Preserving identity and stability is Bhutan’s aim and secularism needs to look at a group approach, apart from individualist approaches. Human rights traditions could acknowledge the cultural-religious roots that inspired them and keep human dignity alive, instead of wanting to remove it from the state altogether and making human rights the new religion. Full article
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27 pages, 3274 KiB  
Article
Supporting Sustainable Development by Identifying Ways to Enhance and Conserve Local Food Wisdom, Loei Province, Thailand
by Supannee Pruksa, Suwaree Sripoona, Suwalee Lowirakorn, Suradech Chaitokkia and Carol Hutchinson
Sustainability 2022, 14(12), 6978; https://doi.org/10.3390/su14126978 - 7 Jun 2022
Cited by 5 | Viewed by 4352
Abstract
Traditional diets are generally nutrient-rich and utilise locally available resources. Strategies to help conserve local food wisdom and increase its value could facilitate a reduction in the burden of all forms of malnutrition and achieve global goals. Our objectives were to: (1) investigate [...] Read more.
Traditional diets are generally nutrient-rich and utilise locally available resources. Strategies to help conserve local food wisdom and increase its value could facilitate a reduction in the burden of all forms of malnutrition and achieve global goals. Our objectives were to: (1) investigate the variety of local foods and food preparation methods in Loei province, Thailand; (2) investigate ways of increasing the nutritional, social, and economic value of local food in this province; and (3) construct a local food database to catalogue and conserve the local food wisdom. Nine out of fourteen districts in Loei province were purposively selected. All were designated key sustainable tourist destinations and represented a range of terrains. There were 423 interview participants, representing all villages in one subdistrict of each of the nine selected districts. We also conducted nine focus group discussions with a total of 90 participants. Data were analysed and categorised, based on the content analysis technique, and we created an online database of the catalogued recipes. There were 240 dishes, placed into seven categories of recipe type. Many of the characteristics of the local dishes from Loei province today remain closely connected to the history of this part of Thailand. We identified that the value of local foods could be improved by investing in technology used for home preservation, actively engaging younger generations to improve the transfer of local food wisdom, investment in technology to utilise local biodegradable materials, and tourist activities based around local food heritage. Future work will involve further development of the local food database, and research to evaluate the application of the database. Moreover, this research can serve as a model for retaining and valuing local food wisdom elsewhere, to promote food security, combat malnutrition, and benefit the local economy. Full article
(This article belongs to the Special Issue Diet, Human Health and Wellbeing in Traditional Food Systems)
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24 pages, 433 KiB  
Article
The Ideology of Patronage and the Question of Identity in the Early Dādūpanth
by Jarosław Zapart
Religions 2022, 13(5), 447; https://doi.org/10.3390/rel13050447 - 16 May 2022
Cited by 2 | Viewed by 2685
Abstract
This article focuses on the Dādūpanth, a religious community centered on the teachings of Dādū Dayāl (1544–1603), a Sant poet of Rajasthan. The aim of the text is to analyze how various forms of patronage affected the formation of the ideology and identity [...] Read more.
This article focuses on the Dādūpanth, a religious community centered on the teachings of Dādū Dayāl (1544–1603), a Sant poet of Rajasthan. The aim of the text is to analyze how various forms of patronage affected the formation of the ideology and identity of this community. The article examines especially the Dādūpanthī ideology of patronage by focusing on the Dādū Janma Līlā (c. 1620), which contains an account of the supposed meeting between Dādū and the emperor Akbar, during which Dādū rejects all offers of patronage. His position needs elucidation as it stands in contrast with the later tendency of the post-17th century Dādūpanth to accept royal and merchant patronage. After analyzing how the hagiography establishes Dādū’s authority and having considered in what types of manuscripts the hagiography was distributed by itinerant preachers, it is suggested that this work is driven by a strong proselytic agenda and that it employs a ‘pedagogical strategy’—represented by the topos of rejected royal support—to establish relationships with merchant patrons. The article concludes with the observation that the increase in royal patronage from the 17th to the 19th century led to changes in the Dādūpanthī ideology that entailed a shift toward a Vaishnava identity. Full article
(This article belongs to the Section Religions and Humanities/Philosophies)
16 pages, 2345 KiB  
Article
Combined Pressure-Driven and Electroosmotic Slip Flow through Elliptic Cylindrical Microchannels: The Effect of the Eccentricity of the Channel Cross-Section
by Pearanat Chuchard and Nattakarn Numpanviwat
Symmetry 2022, 14(5), 999; https://doi.org/10.3390/sym14050999 - 13 May 2022
Cited by 1 | Viewed by 2237
Abstract
Electroosmotic force has been used extensively to manipulate fluid flow in a microfluidic system with various channel shapes, especially an elliptic cylinder. However, developing a computational domain and simulating fluid flow for a system involving an elliptic channel consumes a large amount of [...] Read more.
Electroosmotic force has been used extensively to manipulate fluid flow in a microfluidic system with various channel shapes, especially an elliptic cylinder. However, developing a computational domain and simulating fluid flow for a system involving an elliptic channel consumes a large amount of time. Moreover, the mathematical expression for the fluid velocity of electroosmotic flow in an elliptic channel may be given in the form of the Mathieu functions that have difficulty in achieving the numerical result. In addition, there is clear scientific evidence that confirms the slippage of fluid at the solid-fluid interface in a microscale system. In this study, we present the mathematical model of combined pressure-driven and electroosmotic flow through elliptic microchannels under the slip-fluid condition. From the practical point of view in fluidics, the effect of the eccentricity of the channel cross-section is investigated on the volumetric flow rate to overcome the difficulty. The results show that the substitution of the equivalent circular channel for an elliptic channel provides a valid flow rate under the situation that the areas of both channel cross-sections are equal and the eccentricity of the elliptic cross-section is less than 0.5. Additionally, the flow rate obtained from the substitution is more accurate when the slip length increases or the pressure-gradient-to-external-electric-field ratio decreases. Full article
(This article belongs to the Special Issue Symmetry in Fluid Dynamics)
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18 pages, 4759 KiB  
Article
From Canterbury to the Duero—An Early Example of Becket’s Martyrdom Iconography in the Kingdom of Castile
by Marta Poza Yagüe
Arts 2021, 10(4), 72; https://doi.org/10.3390/arts10040072 - 26 Oct 2021
Viewed by 3546
Abstract
The church of San Miguel of Almazán (Soria, Spain) houses a twelfth-century antependium ornamented with scenes of Thomas Becket’s martyrdom. Discovered during restoration works in 1936, its origin and its original location are unknown. The aim of this article is twofold—to frame its [...] Read more.
The church of San Miguel of Almazán (Soria, Spain) houses a twelfth-century antependium ornamented with scenes of Thomas Becket’s martyrdom. Discovered during restoration works in 1936, its origin and its original location are unknown. The aim of this article is twofold—to frame its manufacture chronologically in light of recent research on late-Romanesque sculpture in Castile, and to use this information to discover who commissioned this work: The bishops of Sigüenza, whose diocese included Almazán? The canons of the monastery of Allende Duero built at the foot of Almazán’s town wall? Or, as has always been claimed, the Castilian Monarchs Alfonso VIII and Eleanor of England, who were the chief promoters of the Becket cult in their dominions? Whatever the answer, this relief is one of the earliest examples of Canterbury saint iconography in the Crown of Castile. Full article
(This article belongs to the Special Issue St. Thomas Becket in Art: Image, Patronage and Propaganda)
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13 pages, 2054 KiB  
Article
Simplified and Rapid Determination of Primaquine and 5,6-Orthoquinone Primaquine by UHPLC-MS/MS: Its Application to a Pharmacokinetic Study
by Waritda Pookmanee, Siriwan Thongthip, Jeeranut Tankanitlert, Mathirut Mungthin, Chonlaphat Sukasem and Supeecha Wittayalertpanya
Molecules 2021, 26(14), 4357; https://doi.org/10.3390/molecules26144357 - 19 Jul 2021
Cited by 5 | Viewed by 2647
Abstract
The method for the determination of primaquine (PQ) and 5,6-orthoquinone primaquine (5,6-PQ), the representative marker for PQ active metabolites, via CYP2D6 in human plasma and urine has been validated. All samples were extracted using acetonitrile for protein precipitation and analyzed using the ultra-high-performance [...] Read more.
The method for the determination of primaquine (PQ) and 5,6-orthoquinone primaquine (5,6-PQ), the representative marker for PQ active metabolites, via CYP2D6 in human plasma and urine has been validated. All samples were extracted using acetonitrile for protein precipitation and analyzed using the ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) system. Chromatography separation was carried out using a Hypersil GOLDTM aQ C18 column (100 × 2.1 mm, particle size 1.9 μm) with a C18 guard column (4 × 3 mm) flowed with an isocratic mode of methanol, water, and acetonitrile in an optimal ratio at 0.4 mL/min. The retention times of 5,6-PQ and PQ in plasma and urine were 0.8 and 1.6 min, respectively. The method was validated according to the guideline. The linearity of the analytes was in the range of 25–1500 ng/mL. The matrix effect of PQ and 5,6-PQ ranged from 100% to 116% and from 87% to 104% for plasma, and from 87% to 89% and from 86% to 87% for urine, respectively. The recovery of PQ and 5,6-PQ ranged from 78% to 95% and form 80% to 98% for plasma, and from 102% to from 112% to 97% to 109% for urine, respectively. The accuracy and precision of PQ and 5,6-PQ in plasma and urine were within the acceptance criteria. The samples should be kept in the freezer (−80 °C) and analyzed within 7 days due to the metabolite stability. This validated UHPLC-MS/MS method was beneficial for a pharmacokinetic study in subjects receiving PQ. Full article
(This article belongs to the Special Issue Application of LC–MS/MS to Biochemistry)
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