Search Results (36,324)

Essential oils have attracted increasing attention due to their bioactive properties. This review focuses on their anticonvulsant potential by exploring the relation between the chemical composition of essential oils and the mechanism of action underlying this effect. Evidence from in vivo and ex vivo studies is presented to identify structure–activity relations and to distinguish well-supported effects from preliminary findings. Moreover, as essential oil’s quality is vital to ensure safety and efficacy in pharmacotherapeutic approaches. For this reason, factors including extraction and analytical methods as well as authenticity assessment are discussed due to their impact on pharmacological consistency and reproducibility. Overall, this review highlights key compounds and mechanisms contributing to anticonvulsant activity, identifies current limitations in the literature, and outlines priorities for future research aimed at validating essential oils as potential complementary therapeutic agents in seizure management. Full article

Background/Objectives: Healthy longevity depends on not only lifespan but also the maintenance of physiological, metabolic, physical, and cognitive functions throughout aging. Identifying early determinants of health is crucial for preventing age-related decline. This narrative review aims to synthesize current evidence on how diet and specific nutrients relate to these early risk factors and indicators of healthy longevity. Methods: A review was performed to identify the links between dietary factors, energy balance, and gut microbiota composition and normal body weight; blood cholesterol, pressure, and glucose; healthy sleep; an active lifestyle; and normal physical function and cognitive performance. Particular attention was given to Mediterranean and other plant-based dietary models as sources of key nutrients. Evidence from observational studies, randomized controlled trials, and meta-analyses was considered. Results: Across all markers, dietary quality and nutrient adequacy emerged as consistent determinants of health outcomes. Key nutrients were associated with favorable cardiometabolic, cognitive, and musculoskeletal functions, such as omega-3 fatty acids, fiber, vitamins D and B, minerals like magnesium and potassium, and polyphenols. Common nutrition gaps included insufficient intake of fiber, unsaturated fats, and micronutrients, which was often linked to a shift toward less plant-based diets. Gut microbiota diversity may mediate several of these associations, influencing metabolism, inflammation, sleep quality, and cognitive performance, although inter-individual variability and causal pathways remain incompletely understood. Conclusions: An integrated dietary approach emphasizing the consumption of whole and plant-rich foods, with moderate amounts of animal foods, supports multiple early markers, risk factors, and indicators of healthy longevity. The modulation of the gut microbiota through plant-based diets and fermented foods represents a promising strategy for maintaining health across aging trajectories. Full article

Background/Objectives: Sustaining a stable and competent workforce in neonatal intensive care units (NICUs) is critical for ensuring high-quality care for vulnerable neonates. However, workforce-related challenges such as job dissatisfaction and turnover remain significant concerns in high-acuity settings. Guided by the ecological model, this study aimed to examine resilience, communication competence, and the nursing work environment as multilevel factors associated with nursing workforce sustainability, with job satisfaction serving as a proxy indicator related to workforce retention and sustainability. Methods: A descriptive cross-sectional survey was conducted among 145 NICU nurses from three tertiary and three general hospitals in South Korea. Data were analyzed using descriptive statistics, independent t-tests, one-way ANOVA, Pearson’s correlation coefficients, and multiple regression analyses using SPSS version 29.0. Results: Job satisfaction was positively associated with resilience (r = 0.67, p < 0.001), communication competence (r = 0.52, p < 0.001), and the nursing work environment (r = 0.57, p < 0.001). Multiple regression analysis indicated that resilience (β = 0.43, p < 0.001), nursing work environment (β = 0.30, p < 0.001), communication competence (β = 0.15, p = 0.040), and employment in a tertiary hospital (β = 0.12, p = 0.038) were significant factors associated with job satisfaction, explaining 55.1% of the variance (adjusted R²) in job satisfaction (F = 30.42, p < 0.001). Conclusions: Job satisfaction, used as a proximal indicator related to workforce sustainability, was associated with multilevel factors across intrapersonal, interpersonal, and organizational domains. Although resilience showed the strongest association, communication competence and the nursing work environment also showed meaningful associations with job satisfaction. These findings highlight the need for integrated, multilevel strategies to support nursing workforce sustainability and sustained nursing practice in NICU settings. Full article

The assessment of sperm quality is essential for improving reproductive efficiency in aquaculture species such as common carp (Cyprinus carpio). While sperm motility is widely used as a key functional indicator, the predictive value of oxidative protein markers for sperm performance remains unclear. In this study, sperm motility and kinematic parameters were assessed using computer-assisted sperm analysis (CASA). Markers of oxidative status, advanced oxidation protein products (AOPPs), ceruloplasmin (CP), and total protein concentration were quantified in samples. The results showed relatively high total motility (70.51%) and progressive motility (69.00%), indicating good sperm quality. No statistically significant correlations (p > 0.0.5) were found between AOPP or CP and sperm motility. Protein concentration was positively associated with motility parameters, including curvilinear velocity (VCL) and amplitude of lateral head displacement (ALH). A significant negative correlation was found between Fulton’s condition factor (FCF) and AOPP (r = −0.686; p < 0.05), indicating that individuals with higher body condition had lower oxidative protein damage. These findings suggest that oxidative protein markers may not directly reflect sperm motility, highlighting the potential value of including additional oxidative stress-related parameters in sperm quality assessment. Full article

Rural land art festivals serve as an important practical vehicle for integrating urban and rural culture and tourism. They constitute a crucial component of rural tourism in China and play a key role in the sustainable development of rural areas. However, in practice, these festivals are generally confronted with the dilemma of superficial tourist participation and insufficient sustainability. This study aims to uncover the intrinsic psychological evolution mechanism underlying tourists’ responses to external stimuli and their value co-creation. The S-O-R model and the two-factor theory are integrated to construct an analytical framework: “external stimulus–psychological sequence–behavioral response.” Using “Modern Fields” as the case study and 437 valid data points, an empirical analysis is conducted with PLS-SEM and artificial neural networks (ANNs). The results indicate that tourist participation is directly driven by destination quality. Content stickiness exerts an indirect influence through perceived value. Perceived value facilitates value co-creation only when it is fully mediated by tourist participation. The path from participation to co-creation is significantly strengthened by restorative environmental perception. A multi-group analysis further reveals that inexperienced tourists exhibit a “stimulus-driven” characteristic, whereas experienced tourists follow a “value internalization” path. The ANN analysis further shows that the strongest nonlinear predictive power for co-creation behavior is held by restorative environmental perception. A significant direct nonlinear effect is also exerted by destination quality. The evolutionary nodes and boundary conditions of tourists’ psychological sequence during this process are revealed. The boundary effect of restorative environmental perception as a catalyst for rural art tourism is demonstrated. A theoretical basis and practical insights are thereby provided for the segmented operation and sustainable development of these activities. Full article

Typical saline lacustrine mixed sedimentary strata are developed in the Middle Permian Lucaogou Formation (P₂l) in the Jimsar Sag, with frequent interbedding of mudstone, dolomitic mudstone, and argillaceous dolomite. The widespread development of dolomite is a key factor controlling the quality of shale oil reservoirs. To reveal the formation mechanism of dolomite in mixed sedimentary rocks and its constraint on lithological assemblages, this study focuses on comparing the differences in mineralogy, geochemistry, and sedimentary environment of the three types of lithologies based on systematic tests such as thin-section observation, X-ray diffraction, major and trace element analysis, organic petrology, and biomarker analysis. The results indicate that dolomite formation in the study area is not controlled by a single factor, but instead results from the combined control of hydrothermal activity, microbial metabolism, and paleoclimatic fluctuations. Hydrothermal activity provided a source of Mg²⁺, and together with evaporation driven by an arid climate, elevated the Mg/Ca ratio of the lake water, establishing the hydrochemical basis favorable for dolomite development. Metabolic activities of lower aquatic organisms, such as bacteria and algae, promoted the formation of a sustained alkaline environment, creating favorable conditions for dolomite precipitation. Against a background of a relatively arid climate, the alternation of extreme arid and extreme precipitation events caused frequent fluctuations in lake water saturation, potentially providing ideal dynamic conditions for rapid and abundant dolomite formation. This combined control governed dolomite development and produced the interbedded lithological succession in the P₂l mixed sedimentary strata. This study integrates the dominant controlling factors and synergistic mechanisms of dolomite development in mixed sedimentary strata of continental saline lacustrine basins, which helps predict the occurrence and distribution of high-quality reservoir lithologies within such strata and has important implications for the optimization of “sweet spots” in shale oil exploration. Full article

mRNA vaccines are a relevant approach in cancer immunotherapy, using messenger RNA to induce immune responses against tumor-associated antigens. In this review, BNT111 and BNT122 are compared as representative off-the-shelf and personalized models. BNT111 is a fixed mRNA vaccine that has demonstrated significant antitumor efficacy against shared melanoma antigens, particularly when combined with immune checkpoint inhibitors. It allows a standardized production via in vitro transcription (IVT) in a cell-free system. Conversely, BNT122 is a personalized vaccine designed to match an individual’s tumor mutations by targeting patient-specific neoantigens to elicit more robust immune responses. It has significant suitability in the adjuvant setting to target minimal residual disease. Despite favorable safety and immunogenicity, the effectiveness of these vaccines is influenced by various factors, including tumor heterogeneity, differences in antigen expression, off-target effects on mRNA-LNP distribution, molecular instability, and complex manufacturing constraints. Neither approach can be directly considered as the definitive optimal vaccine. A comprehensive analysis of their strengths and limitations is vital for a balanced and objective future research direction. Collectively, this emphasizes the need for further improvements in vaccine design and strategies, prioritizing high-quality, safe, and accessible treatments for every cancer-based patient and ensuring their successful integration into healthcare. Full article

Soil salinization is increasingly threatening global agricultural productivity and food security, currently affecting over 6% of the world’s land and one-third of irrigated areas. Tomato (Solanum lycopersicum L.), a major vegetable crop worldwide, exhibits moderate sensitivity to salinity, which limits both its yield and fruit quality. In recent years, epigenetic regulation has gained attention as a key mechanism enabling flexible and reversible control of gene expression without altering DNA sequences. This review synthesizes current knowledge on the epigenetic control of salt stress responses in tomato, focusing on three interconnected levels: DNA methylation dynamics, RNA-directed DNA methylation (RdDM), and histone modifications. We explore how DNA methyltransferases reshape the methylome under salinity, using examples such as PKE1 and SlGI to illustrate functional gene-body methylation. The RdDM pathway is discussed with emphasis on the unexpected role of SlAGO4A as a negative modulator of stress tolerance and the growing evidence for RdDM-mediated regulation of transcription factors. We also examine the balanced regulation of histone acetylation and deacetylation, highlighting the conserved role of GCN5 in maintaining cell wall integrity and the diverse functions of histone deacetylases, such as SlHDA1, SlHDA3, and SlHDA5, in stress adaptation. Additionally, insights from wild tomato species and grafting-induced epigenetic changes are presented, revealing new dimensions of stress memory. Collectively, these epigenetic mechanisms constitute a complex regulatory framework that integrates stress responses with growth and development, providing potential targets for epigenetic breeding of salt-tolerant tomatoes. Full article

Ecosystem service assessment provides a critical basis for optimizing regional ecological management and promoting sustainable development. From the water–land–carbon coupling perspective, this study established a technical framework for quantifying individual services, coupling a composite index, and analyzing multidimensional driving mechanisms. The InVEST model was applied to quantify three core ecosystem services: water yield, habitat quality, and carbon storage. A Composite Ecosystem Service Index (CESI) was constructed through normalization and weighted summation. Multidimensional driving factors were identified using the Optimal Parameter-Based Geographical Detector. Taking Ningxia during 2004–2024 as the study area, the results showed that the CESI exhibited a fluctuating upward trend with significant spatial heterogeneity, characterized by a south–high and north–low pattern. Land use transitions were dominated by bidirectional conversions between cropland and grassland, while impervious area expanded rapidly and barren land decreased overall. The spatial differentiation of CESI was jointly controlled by natural and anthropogenic factors, with land use type, precipitation, and digital elevation model showing the strongest explanatory power, and all two-factor interactions displaying pronounced enhancement effects. This study provides a reproducible framework for ecosystem service assessment in arid and semi-arid regions, supporting ecological restoration, land use optimization, and the coordinated development of ecology and economy under water–land–carbon synergy. Full article

To maintain homeostatic conditions and optimal function during stressors, mitochondria initiate retrograde signaling. The mitochondrial integrated stress response (ISR) and unfolded protein response (UPR^mt) are critical quality control mechanisms activated during instances of mitochondrial perturbations. Restoration of mitochondrial homeostasis is orchestrated by three transcription factors, ATF4, CHOP, and ATF5, which upregulate protective genes to counteract stress. As the health and function of skeletal muscle are heavily dependent on a highly adaptive mitochondrial network, defining how mitochondrial health is maintained across various conditions is essential. Although several studies demonstrate the importance of these responses following instances of stress, the signaling mechanisms required to initiate such pathways remain poorly characterized in skeletal muscle. This review examines how the mitochondrial ISR/UPR^mt and related transcription factors respond to organellar stress by emphasizing the molecular events that occur during exercise, aging and muscle disuse. By consolidating the literature, this work aims to highlight the current understanding of mitochondrial stress response signaling within skeletal muscle and thus emphasize areas for future research and potential therapeutic strategies during divergent metabolic conditions. Full article

Fruit shape is determined by patterns of cell division and expansion during early development, yet the upstream transcription factors coordinating cell wall dynamics and cytoskeletal organization remain largely unknown. Here, we report that SlbHLH113, a bHLH transcription factor, positively regulates tomato fruit elongation. Overexpression (OE) of SlbHLH113 produced elongated fruits with increased length/width ratio, whereas RNAi lines exhibited flattened fruits. Histological analysis revealed that SlbHLH113 alters columella cell polarity—promoting elongated cell morphology without affecting cell area—and reduces columella–placenta width and locule width, without altering pericarp thickness. Transcriptomic profiling identified 87 differentially expressed genes in OE lines, with enrichment in cell wall-related processes. Notably, a pectate lyase gene (PL5) and an expansin gene (EXT90) were down-regulated, while genes involved in oriented cellulose deposition (COBRA4) and ethylene signaling were up-regulated. Importantly, SlbHLH113 physically interacts with the microtubule-associated protein SlIQD21a, as demonstrated by yeast two-hybrid and luciferase complementation assays. Finally, SlbHLH113 did not affect major nutrient contents in red-ripe fruits. Collectively, our findings identify SlbHLH113 as a novel regulator of tomato fruit shape that might act through cell polarity control, cell wall remodeling, and interaction with a microtubule-associated protein, offering a potential target for improving fruit morphology without compromising nutritional quality. Full article

Groundwater is the primary source of livestock watering across the arid pasturelands of western Kazakhstan, yet no systematic field hydrochemical assessment has been published for this region in over 40 years. This study presents the first systematic field-based hydrochemical characterisation of groundwater sources used for pasture livestock watering in the West Kazakhstan Region and Aktobe Region, filling a critical data gap that has persisted since the Soviet era. Specifically, it characterises the hydrochemistry, water quality, and infrastructure condition of groundwater sources, and evaluates the groundwater resource potential against current and projected livestock water demand. A total of 139 groundwater samples were collected along 11,182 km of field routes during May–July 2025, and analysed for 25 physicochemical parameters; hydrochemical classification was performed using AquaChem 11, and spatial analysis was conducted in ArcGIS 10.8. The groundwater chemistry distribution is bimodal: fresh bicarbonate-calcium-magnesium waters (TDS < 3.0 g/L) constitute approximately 80% of samples, while highly mineralised chloride-sulphate-sodium waters (TDS up to 9.91 g/L) occur in salt-dome-influenced discharge zones. Nitrate concentrations exceeded 50 mg/L in 23–36% of samples, with maxima of 635 mg/L, reflecting intensive anthropogenic contamination near livestock facilities. Predictive exploitable fresh groundwater resources exceed current livestock demand by a factor of 162. The principal constraint on pasture water supply is not resource scarcity but the non-operational status of 51–75% of inspected watering infrastructure, a legacy of post-Soviet institutional collapse that requires urgent rehabilitation. Full article

Digital innovation constitutes the core determinant of sustainable digital transformation and functions as a pivotal driver of regional high-quality economic development. As a strategic node in China’s urban agglomeration framework, the Guangdong–Fujian–Zhejiang Coastal Urban Agglomeration plays a critical role in regional synergistic high-quality development. This study examines how digital innovation impacts the coupling coordination of high-quality development in the urban agglomeration. The results show: (1) Synergistic high-quality development shows a steady downward trend in the research region with substantial coordination potential. (2) “Gradient disparity” exists, primarily driven by inter-regional Gini coefficient contributions. (3) Overall coupling coordination remains at the antagonistic stage with significant convergence tendencies. (4) Lack of robust central city and centripetal force hinders effective spatial radiation. Driven by the core-periphery spatial differentiation, the short-run dominance of digital innovation’s polarization effect undermines the coordination level of urban agglomerations through scale expansion, structural optimization, and technological empowerment. It requires vigilance against Yangtze and Pearl River Delta siphoning effects. This study provides theoretical and practical implications for promoting digitally driven, synergistic, sustainable, and balanced high-quality development, as well as for optimizing policy frameworks in the new era. Full article

Teacher coaching is a widely used approach to support teacher professional development, yet the relational and psychological factors that influence coaching outcomes remain underexplored. Guided by the Expectancy-Value Theory and prior literature, this study examined the role of coaching relationships, teacher self-efficacy, and teachers’ motivation for coaching in the coaching process, with two key outcomes: teachers’ acceptability of coaching and perceived stress. A sample of 308 K-12 teachers from the United States completed relevant measures. Structural equation modeling revealed that high-quality coaching relationships were significantly associated with greater acceptability, self-efficacy, and motivation, particularly increased perceived benefits and reduced reservations. Coaching relationships and coaching acceptability were indirectly associated via self-efficacy and perceived benefits. The indirect association between coaching relationships and stress was fully explained through self-efficacy. These findings underscore the importance of fostering strong relationships with teachers and addressing motivational components to enhance the effectiveness of coaching interventions. Full article

Osteoporosis and hyperglycemia are increasingly recognized as dual public health concerns in T1DM. However, the precise molecular mechanisms by which exercise ameliorates these conditions, particularly the contribution of mechanosensitive channels such as Piezo1, remain incompletely elucidated. To explore these mechanisms, T1DM mice were subjected to a 6-week treadmill training protocol (15 m/min, 20 min/day, 6 days/week) to evaluate the functions of exercise on diabetic osteoporosis and hyperglycemia. Exercise intervention markedly improved bone quality in T1DM mice, alleviating osteoporotic manifestations, as evidenced by enhanced mechanical strength, restored bone microarchitecture, and normalized histomorphology. Concurrently, exercise significantly reduced hyperglycemia. To clarify the role of Piezo1, mechanical stretch was applied to Piezo1-knockout MC3T3-E1 (Piezo1^−/−) cells in vitro, mimicking the mechanical stimulation induced by exercise. Consistent with the in vivo results, mechanical stimulation facilitated osteogenic differentiation and glucose metabolism through Piezo1-mediated mechanotransduction. Importantly, these beneficial effects were substantially abrogated in Piezo1^−/− cells, highlighting the central role of Piezo1. Collectively, these findings demonstrate that Piezo1-mediated mechanotransduction constitutes a critical factor by which exercise mitigates osteoporosis and hyperglycemia in T1DM mice. This study provides a framework for the development of new therapeutic strategies targeting Piezo1-mediated mechanotransduction for T1DM management. Full article