Search Results (36)

This study investigated the effects of plateau pika (Ochotona curzoniae) disturbances and altitude on soil organic carbon (SOC) storage characteristics, including SOC content and SOC density (SOCD). In this study, plateau pika outbreak areas and non-outbreak areas at different altitudes were compared in terms of vegetation biomass, soil physicochemical properties, SOC content and SOCD to establish the relationship between vegetation and soil characteristics (including SOC content and SOCD). The results showed that SOC and SOCD decreased significantly (p < 0.01) in plateau pika outbreak areas, but SOCD increased first and then decreased with elevation in non-outbreak areas. Soil total nitrogen (TN) content decreased significantly (p < 0.01) with elevation in both plateau pika outbreak and non-outbreak areas. There were significant differences (p < 0.05) in total phosphorus (TP) at low elevations and nitrate nitrogen (NO₃⁻-N) at high elevations between outbreak and non-outbreak areas, but other nutrients did not differ hugely between outbreak and non-outbreak areas at the same elevation. Correlation analysis revealed that belowground biomass (BGB) in the plateau pika outbreak area was significantly and positively correlated with SOC (p < 0.01); structural equation modeling (SEM) analysis revealed that altitude had a direct effect on SOC (path coefficient = −0.882, p < 0.001) in the plateau pika outbreak area, but only a reduced influence on SOC and SOCD in the non-outbreak area; nitrate nitrogen in the plateau pika outbreak area and TN were the key influencing factors, which exerted a strong direct influence on SOC and SOCD (path coefficient = −0.666 and 0.639 (p < 0.001), respectively). Therefore, increasing vegetation biomass and nitrogen nutrient content through reseeding pasture and fertilization can facilitate the accumulation and recovery of SOC and SOCD in the ecological restoration of degraded alpine meadows, and it is especially important to quickly enrich soil nitrogen content in the outbreak area of plateau pika populations at high altitudes. Full article

The Tibetan Plateau is a critical ecological habitat where the overpopulation of plateau pika (Ochotona curzoniae), a keystone species, accelerates grassland degradation through excessive burrowing and herbivory, threatening ecological balance and human activities. To address the inefficiency and high costs of traditional pika burrow monitoring, this study proposes an intelligent monitoring solution that integrates drone remote sensing with deep learning. By combining the lightweight visual Transformer architecture EfficientViT with the hybrid attention mechanism CBAM, we develop an enhanced YOLOv11-AEIT algorithm: (1) EfficientViT is employed as the backbone network, strengthening micro-burrow feature representation through a multi-scale feature coupling mechanism that alternates between local window attention and global dilated attention; (2) the integration of CBAM (Convolutional Block Attention Module) in the feature fusion neck reduces false detections through dual-channel spatial attention filtering. Evaluations on our custom PPCave2025 dataset show that the enhanced model achieves a 98.6% mAP@0.5, outperforming the baseline YOLOv11 by 3.5 percentage points, with precision and recall improvements of 4.8% and 7.2%, respectively. The algorithm enhances efficiency by a factor of 15 compared to manual inspection, while seamlessly meeting real-time drone detection requirements. This approach provides high-precision yet lightweight technical support for plateau ecological conservation and serves as a valuable methodological reference for similar ecological monitoring tasks. Full article

The comparative analysis of the feeding ecology among sympatric small carnivores reveals both differentiation and overlap in resource utilization patterns, which serves as a critical pathway for understanding interspecific interactions and maintaining ecosystem stability. In this study, we collected fecal samples from sympatric Pallas’s cats (Otocolobus manul, n = 26) and red foxes (Vulpes vulpes, n = 13) within the Sanjiangyuan National Park (SNP) in China. Subsequently, DNA barcoding technology was employed to analyze the dietary composition and interspecific differences of these two small carnivores. The results demonstrated that both species primarily prey on plateau pikas (Ochotona curzoniae) and small rodents. Despite a high trophic niche overlap between Pallas’s cats and red foxes (O_jk = 0.81), interspecific competition is mitigated through differentiate feeding proportions of shared prey species. Furthermore, the trophic niche breadth of red foxes (B = 267.89) exceeds that of Pallas’s cats (B = 162.94), reflecting a greater diversity of prey resources utilized by red foxes. Consequently, the two small carnivores achieve sympatric coexistence via differentiated resource utilization. These findings enhance our understanding of the coexistence mechanisms within carnivore communities and provide a scientific basis for the conservation of wildlife in the SNP. Full article

Plateau pika (Ochotona curzoniae, hereafter pika) is a key species in the alpine grasslands on the Qinghai-Tibetan Plateau (QTP). They are susceptible to the influence of external disturbance and may present great variation, which is important to evaluate their ecological role in alpine grasslands. However, our knowledge regarding their interannual variation and the influencing mechanism is still limited due to the lack of long-term observation of pika density. This study aimed to investigate the spatiotemporal variations in pika and the associated key influencing factors by aerial photographing at 181 sites in Gannan Tibetan Autonomous Prefecture in 2016, 2019, and 2022. Our findings showed that: (1) pika primarily distributed in the central and northeastern Maqu County and the southwestern part of Luqu County, and their average density was in a range of 9.87 ha⁻¹ to 14.43 ha⁻¹ from 2016 to 2022; (2) high pika density were found in 1.22 to 3.61 °C for annual mean temperature, 12.86 to 15.06 °C for diurnal temperature range, 3400 to 3800 m for DEM and less than 3° for slope; and (3) pika density showed varied response to interannual changes in mean diurnal range, annual precipitation and precipitation of the driest month in different years. Our results concluded that pika density showed significant spatiotemporal variations, and climate and terrain variables dominantly affected pika density. Given the great interannual fluctuation of climate variables and different responses of pika density to these variables, our results suggested that long-term monitoring of pika is crucial to reveal their real distribution, response mechanism to habitat environment, and role in alpine grasslands. Moreover, unmanned aerial vehicles are cost-effective tools for the long-term monitoring of pika. Full article

This study investigates the foraging habits and dietary niche characteristics of plateau pikas (Ochotona curzoniae) by analyzing differences between the cold and warm seasons using stable isotope analysis of carbon and nitrogen in liver, muscle, and fur organs. The results indicated that in the cold season, the plant species incorporated into different organs were more diverse than in the warm season, with varying contribution rates for each plant species. Potentilla anserina made the most significant contribution to the plateau pika’s diet during the cold season, while Kobresia humilis had the highest contribution in the warm season. The trophic niche width of liver and muscle organs was broader in the cold season compared to the warm season, while the trophic niche width of the fur organ was narrower in the cold season. Additionally, long-term dietary information derived from muscle and fur organs showed a wider niche breadth compared to short-term dietary information obtained from the liver organ. Across both seasons, organ niche overlap was highest in fur, followed by muscle, and lowest in the liver. In conclusion, short-term food source information (derived from the liver organ) indicated fewer food types and a smaller niche breadth. In contrast, long-term food source information (obtained from muscle and fur organs) included a greater variety of food types and a larger niche breadth. Additionally, niche overlap was more pronounced on the long-term scale compared to the short-term scale. These findings highlight the adaptive feeding strategies of plateau pikas in response to varying seasonal food availability and the ecological importance of their dietary flexibility in maintaining energy balance in challenging environments. Full article

The plateau pika (pl-pika), a resilient mammal of the Qinghai-Tibet Plateau, exhibits remarkable adaptations to extreme conditions. This study delves into mutations within the Endothelial PAS Domain Protein 1 (EPAS1) gene, crucial for high-altitude survival. Surprisingly, a novel 6-bp insertion/deletion (indel) mutation in EPAS1’s Intron 13, along with an additional repeat unit downstream, was discovered during PCR amplification. Genetic analysis across altitude gradients revealed a correlation between this indel’s frequency and altitude, hinting at its role in altitude adaptation. Fluorescence enzyme assays unveiled enhancer activity within Intron 13, where the deletion of repeat units led to increased activity, indicating potential transcription factor binding. Notably, GCM1 emerged as a candidate transcription factor binding to the indel site, suggesting its involvement in EPAS1 regulation. These findings enrich our comprehension of high-altitude adaptation in plateau pikas, shedding light on the intricate interplay between genetic mutations, transcriptional regulation, and environmental pressures in evolutionary biology. Full article

Rodent infestation has become one of the important factors in grassland degradation on the Qinghai–Tibet Plateau, one of the hindrances to ecological and environmental protection, and a threat to the balance and development of the ecosystem in the Sanjiangyuan region. Based on the need for the scientific planning for ecological protection, this paper designs a method for detecting rodent infestation in plateau scenarios. Firstly, data were collected and annotated, and a dataset of plateau rodent distribution in the Qinghai region was constructed. The collected data include videos captured through drone-based field surveys, which were processed using OpenCV and annotated with LabelMe. The dataset is categorized into four specific types: ungobbled rat holes, gobbled rat holes, rocks, and cow dung. This categorization allows the model to effectively differentiate between rodent-related features and other environmental elements, which is crucial for the segmentation task. Secondly, the latest segmentation algorithm provided by YOLO v8 is improved to design a segmentation algorithm that can accurately detect the distribution of rodent infestation in the plateau scene. The specific improvements are as follows: firstly, the Contextual Transformer module is introduced in YOLO v8 to improve the global modeling capability; secondly, the DRConv dynamic region-aware convolution is introduced in YOLO v8 to improve the convolutional representation capability; thirdly, the attention mechanism is incorporated in the backbone of YOLO v8 to enhance the feature extraction capability of the network capability. A comparison test with the original algorithm on the plateau rodent distribution dataset showed that the new algorithm improved the detection accuracy from 77.9% to 82.74% and MIoU from 67.65% to 72.69% on the plateau rodent distribution dataset. The accuracy of the evaluation of plateau rodent damage levels has been greatly improved. Full article

Plateau pika (Ochotona curzoniae) is crucial to soil organic carbon (SOC) storage in the Qinghai–Tibetan plateau (QTP), but its role in bacterial SOC metabolisms across different degraded alpine grasslands remains unclear. In this study, we investigated the soil physicochemical properties and the composition and function of the bacterial communities in control and pika-disturbed grasslands experiencing different degradation levels (undegraded, UDM; lightly, LDM; moderately, MDM and severely, SDM). The results demonstrate that (i) the primary bacterial phyla include Proteobacteria, Acidobacteriota, Actinobacteriota, Bacteroidota and Verrucomicrobiota. Soil physicochemical properties significantly impact the composition of the bacterial communities and determine the influence of pika disturbance. Pika disturbance increases bacterial OTUs by 7.5% in LDP (p > 0.05) and by 50.5% in MDP (p < 0.05), while decreases OTUs by 21.4% in SDP (p < 0.05). (ii) Pika disturbance downregulates the exoenzyme abundance associated with simple and complex organic matter decomposition by 9.5% and 13.9% in LDP, and 29.4% and 26.3% in MDP (p < 0.05), while upregulates these exoenzymes by 23.6% and 37.9% in SDP (p < 0.05). These changes correspond to the increase in TC and SOC in LDP and MDP but declines in SDP. (iii) Plateau pika disturbance can enhance SOC accumulation through upregulating the C cycle pathway of ethanol production in LDP and MDP. However, it upregulates the pathway of pyruvate to CO₂ conversion in SDP, leading to negative influence on SOC storage. Full article

We aimed to investigate the species composition of a small mammal community and the prevalence of Echinococcus spp. in a typical endemic area of the Tibetan Plateau. One pika and five rodent species were identified based on the morphological characteristics of 1278 small mammal specimens collected during 2014–2019. Detection of Echinococcus DNA in tissue samples from small mammal specimens revealed that Ochotona curzoniae (pika, total prevalence: 6.02%, 26/432), Neodon fuscus (5.91%, 38/643), N. leucurus (2.50%, 3/120), and Alexandromys limnophilus (21.74%, 10/46) were infected by both E. multilocularis and E. shiquicus; Cricetulus longicaudatus (16.67%, 1/6) was infected by E. shiquicus; and no infection was detected in N. irene (0/15). Neodon fuscus and O. curzoniae were the two most abundant small mammal species. There was no significant difference in the prevalence of pika and the overall rodent species assemblage (6.26%, 53/846); however, the larger rodent populations suggested that more attention should be paid to their role in the transmission of echinococcosis in the wildlife reservoir, which has long been underestimated. Moreover, although DNA barcoding provides a more efficient method than traditional morphological methods for identifying large numbers of small mammal samples, commonly used barcodes failed to distinguish the three Neodon species in this study. The close genetic relationships between these species suggest the need to develop more powerful molecular taxonomic tools. Full article

As one of the dominant species of the alpine grassland on the Qinghai–Tibet Plateau, the activities (e.g., gnawing, burrowing, and grass storage) of plateau pikas (Ochotona curzoniae) directly alter the plant community structure of the grassland ecosystem and affect livestock production and greenhouse gas emission. In order to investigate the effects of rodent isolation (RI) on plant community structure and greenhouse gas emission in the alpine grassland of the Qinghai–Tibet Plateau, we established plots of rodent isolation and rodent activity (i.e., the control sample (CK)) in the 14th village, Seni District, Nagqu City in May 2018. From July 2019 to September, the numbers, sizes, and total damaged area of effective holes; the height, coverage, and aboveground plant biomass; and the methane (CH₄) and nitrous oxide (N₂O) emissions of the alpine grassland were monitored by the quadrat survey method and static closed-chamber method. The results show that the invasion and tunneling of Ochotona curzoniae resulted in the destruction of alpine grassland measuring 0.064 m² per square meter, while the rodent isolation plots showed that 97.9% of the alpine grassland remained unaltered; such unaffected land implies that the economic income of herdsmen could increase by 140 CNY hm⁻². The rodent isolation plots also show that the height and proportion of grasses and sedges in the alpine grassland increased, while the proportion of poisonous weeds decreased. Moreover, the rodent isolation plots also showed a significantly increased coverage of aboveground biomass (p < 0.05), although species richness showed no significant effect based on the Shannon–Weiner, Simpson, and Pielou indices (p > 0.05). The soil uptake of CH₄ and N₂O was 204.99 ± 50.23 μg m⁻² h⁻¹ and 4.48 ± 1.02 μg m⁻² h⁻¹ in the rodent isolation plots, significantly higher by 465.75% and 3001.4% relative to the rodent activity plots, respectively (p < 0.05). Therefore, the establishment of rodent isolation areas can effectively alleviate the degree of damage to alpine grasslands in the short run and slow down the greenhouse gas emission rate to some extent. However, excessive rodent control may also have negative effects on grassland ecosystems, so more attention should be paid in future studies to determining the disturbance threshold of plateau pika in this area. These results provide theoretical guidance for rodent control, grassland protection, and ecological environment management on the Qinghai–Tibet Plateau. Full article

The yak (Poephagus grunniens) has evolved unique adaptations to survive the harsh environment of the Qinghai–Tibetan Plateau, while their gut microorganisms play a crucial role in maintaining the health of the animal. Gut microbes spread through the animal population not only by horizontal transmission but also vertically, which enhances microbial stability and inheritance between generations of the population. Homogenization of gut microbes in different animal species occurs in the same habitat, promoting interspecies coexistence. Using the yak as a model animal, this paper discusses the adaptive strategies under extreme environments, and how the gut microbes of the yak circulate throughout the Tibetan Plateau system, which not only affects other plateau animals such as plateau pikas, but can also have a profound impact on the health of people. By examining the relationships between yaks and their gut microbiota, this review offers new insights into the adaptation of yaks and their ecological niche on the Qinghai–Tibetan plateau. Full article

Burrowing animals are a critical driver of terrestrial ecosystem functioning, but we know little about their effects on soil microbiomes. Here, we evaluated the effect of burrowing animals on microbial assembly processes and co-occurrence patterns using soil microbiota from a group of habitats disturbed by Plateau pikas (Ochtona curzoniae). Pika disturbance had different impacts on bacterial and fungal communities. Fungal diversity generally increased with patch area, whereas bacterial diversity decreased. These strikingly different species–area relationships were closely associated with their community assembly mechanisms. The loss of bacterial diversity on larger patches was largely driven by deterministic processes, mainly due to the decline of nutrient supply (e.g., organic C, inorganic N). In contrast, fungal distribution was driven primarily by stochastic processes that dispersal limitation contributed to their higher fungal diversity on lager patches. A bacterial co-occurrence network exhibited a positive relationship of nodes and linkage numbers with patch area, and the fungal network presented a positive modularity–area relationship, suggesting that bacteria tended to form a closer association community under pika disturbance, while fungi tended to construct a higher modularity network. Our results suggest that pikas affects the microbial assembly process and co-occurrence patterns in alpine environments, thereby enhancing the current understanding of microbial biogeography under natural disturbances. Full article

Captivity is an important and efficient technique for rescuing endangered species. However, it induces infertility, and the underlying mechanism remains obscure. This study used the plateau pika (Ochotona curzoniae) as a model to integrate physiological, metagenomic, metabolomic, and transcriptome analyses and explore whether dysbiosis of the gut microbiota induced by artificial food exacerbates infertility in captive wild animals. Results revealed that captivity significantly decreased testosterone levels and the testicle weight/body weight ratio. RNA sequencing revealed abnormal gene expression profiles in the testicles of captive animals. The microbial α-diversity and Firmicutes/Bacteroidetes ratio were drastically decreased in the captivity group. Bacteroidetes and Muribaculaceae abundance notably increased in captive pikas. Metagenomic analysis revealed that the alteration of flora increased the capacity for carbohydrate degradation in captivity. The levels of microbe metabolites’ short-chain fatty acids (SCFAs) were significantly high in the captive group. Increasing SCFAs influenced the immune response of captivity plateau pikas; pro-inflammatory cytokines were upregulated in captivity. The inflammation ultimately contributed to male infertility. In addition, a positive correlation was observed between Gastranaerophilales family abundance and testosterone concentration. Our results provide evidence for the interactions between artificial food, the gut microbiota, and male infertility in pikas and benefit the application of gut microbiota interference in threatened and endangered species. Full article

Although fungi occupy only a small proportion of the microbial community in the intestinal tract of mammals, they play important roles in host fat accumulation, nutrition metabolism, metabolic health, and immune development. Here, we investigated the dynamics and assembly of gut fungal communities in plateau pikas inhabiting six altitudinal gradients across warm and cold seasons. We found that the relative abundances of Podospora and Sporormiella significantly decreased with altitudinal gradients in the warm season, whereas the relative abundance of Sarocladium significantly increased. Alpha diversity significantly decreased with increasing altitudinal gradient in the warm and cold seasons. Distance-decay analysis showed that fungal community similarities were significantly and negatively correlated with elevation. The co-occurrence network complexity significantly decreased along the altitudinal gradients as the total number of nodes, number of edges, and degree of nodes significantly decreased. Both the null and neutral model analyses showed that stochastic or neutral processes dominated the gut fungal community assembly in both seasons and that ecological drift was the main ecological process explaining the variation in the gut fungal community across different plateau pikas. Homogeneous selection played a weak role in structuring gut fungal community assembly during the warm season. Collectively, these results expand our understanding of the distribution patterns of gut fungal communities and elucidate the mechanisms that maintain fungal diversity in the gut ecosystems of small mammals. Full article

The plateau pika (Ochotona curzoniae) actively contributes to soil erosion and meadow degradation in western China’s Yellow River source zone. This study aimed to elucidate the effects of the pika mound numbers on the hydrodynamic characteristics and soil water erosion through simulated rainfall experiments. The inhibition effects of restored vegetation growth on meadow degradation and soil erosion were explored using a revegetated pika mound as a control. The results showed that at a rainfall intensity of 30 mm/h, the soil loss per unit time increased and then decreased with rainfall time and that 15–20 min of rainfall duration was the sensitive period for soil loss in different pika mound patch lands. The degradation of meadows due to the activities of plateau pika is an essential factor influencing soil erosion, and the soil erosion rate is positively correlated with both the pika mound numbers and slope. The mean flow velocity can better describe the process of the soil erosion, and its value increased with the number of pika mounds and slope. The Reynolds number ranged from 57.85 to 153.63 (Re <500), and it was preliminarily determined that all slope runoff was laminar flow. The Froude number increased linear function with pika mound numbers (p < 0.01), and both the pika mound numbers and slope were significant factors affecting it (p < 0.05). The Darcy–Weisbach resistance coefficient instead decreased with the pika mound numbers and slope, and the inhibitory effect of vegetation on soil erosion was probably limited when the mound numbers reached a certain level. According to the results of the grey correlation and Pearson correlation analysis, changes in the number of pika mounds led to variability in the soil water erosion properties by altering the landscape scale effect. The number of pika mound patches (NP), edge length index (TE), area index (AREA), and volume index (V) were the key influencing factors on the soil erosion rate and hydrodynamic parameters. We conclude that plateau pika activities intensify meadow degradation, and the continuous increase in pika mounds decreases the vegetation cover and flow resistance and increases soil erodibility. Controlling the intensity of plateau pika activities will effectively prevent soil erosion in degraded areas. Full article