Search Results (1,275)

To investigate the effects of habitat degradation on the genomic level, young of the year (YOY) bluefish, (Pomatomus saltatrix) from the highly urbanized Hudson Raritan Estuary (HRE) and the less impacted Mullica River-Great Bay (MRGB) estuary were analyzed for condition, level of PCBs (Polychlorobiphenyls), and pesticides, and compared in a preliminary transcriptomic study. For chemical analysis, whole juvenile fish were freeze-dried, extracted in DCM (Dichloromethane), extracts were cleaned by florisil/alumina/silica column chromatography and size-exclusion HPLC, and quantified by gas chromatography. For transcriptomic analysis, Suppression Subtractive Hybridization (SSH) libraries from the pooled livers of fish from the two sites were constructed to subtract common transcripts and recover cloned expressed sequence tag (EST) transcripts that were putatively more highly expressed in one or the other environment. Transcripts were Sanger-sequenced and identified by bioinformatic analysis. Relative quantitative PCR (polymerase chain reaction) was used to investigate a subset of the selected transcripts. MRGB fish were larger and in better condition than HRE fish. HRE fish had PCB concentrations that were, in sum, about seven times higher than MRGB fish and were more highly contaminated with pesticides including Dichlorodiphenyltrichloroethane (DDT) and its metabolites. Transcripts related to metabolic, transport, immune, detoxification, signaling, and environmental response and additional functions were associated with contrasting habitats and provide gene targets to investigate the hypothesis that the growth and condition of the HRE bluefish may be limited by their poor environmental quality. Full article

Ensuring food safety requires the detection of trace-level contaminants such as pesticides, mycotoxins, and heavy metals. Analytical approaches for these analytes should feature high sensitivity, good selectivity, and compatibility with aqueous matrices; surface-enhanced Raman spectroscopy (SERS) satisfies these requirements. Addressing the absence of a unified comparative analytical framework, this critical review surveys recent SERS-enabled sensing strategies for food contaminants. Detection strategies differ substantially across the three contaminant classes: pesticides can be directly detected at ppb levels through substrate engineering and deep learning; mycotoxins rely on affinity-recognition elements to reach pg-mL-level sensitivity; and Raman-inactive heavy metals demand indirect readout via functional probes. Crucially, despite these divergent analytical routes, the field confronts three shared bottlenecks—spectral irreproducibility, severe matrix interference, and the lack of standardized protocols, all of which hinder regulatory adoption. Compared with near-infrared spectroscopy (NIR) and hyperspectral imaging (HSI), SERS delivers outstanding sensitivity for confirmatory trace-level analysis, while its limited throughput may be compensated by multispectral data fusion. Future advances should prioritize portable sensing hardware, explainable Artificial Intelligence (AI), and multiplexed detection to transfer laboratory-scale sensitivity toward practical field-deployable testing tools. Full article

Seed-associated microorganisms influence seed quality, seedling establishment, and plant health and also constitute a major source of seed-borne pathogens. Cold atmospheric plasma (CAP) has emerged as a promising alternative to chemical seed treatments because of its antimicrobial activity, although its effects on fungal communities associated with developing seedlings remain poorly understood. Here, Arabidopsis thaliana seeds from two ecotypes (Columbia and Landsberg erecta) were exposed to CAP for 5 or 15 min. Seed decontamination efficiency was assessed by culturing on malt extract agar and nephelometric analyses, while fungal communities associated with seedlings derived from treated and untreated seeds were characterized by ITS1 amplicon sequencing. CAP efficiently reduced fungal contamination without affecting seed germination. CAP altered the composition of fungal communities associated with developing seedlings, but the magnitude of these changes depended on seed batch and ecotype. Dominant taxa markedly declined after treatment, whereas several low-abundance taxa increased in relative abundance. These findings demonstrate that CAP is an effective pesticide-free technology for seed decontamination and can also reshape fungal communities associated with developing seedlings, highlighting broader ecological consequences of plasma-based seed treatments. Full article

Pesticide contamination of food and environmental matrices represents a potential risk to human health. This study investigated the toxicological effects of commercial glyphosate and dicamba-based herbicide formulations, individually and in combination, on human intestinal epithelial Caco-2 cells. Cells were exposed to a concentration range of 0.1–10,000 mg/L for 24, 48, and 72 h to determine IC₅₀ values. Subsequent assays were conducted using concentrations based on the lowest IC₅₀ obtained and in the limits established by the Environmental Protection Agency for drinking water. After 48 h of exposure, glyphosate at the highest tested concentration, as well as co-exposure to glyphosate and dicamba, induced significant cytotoxicity, modulation of oxidative stress parameters, and alterations in antioxidant defenses, accompanied by increased rates of apoptosis and necrosis. Dicamba exposure alone also resulted in elevated apoptotic and necrotic cell populations. A reduction in N-acetyl-β-D-glucosaminidase activity was observed across most tested concentrations, suggesting impaired inflammatory response capacity. This work identified alterations in Caco-2 that impair cellular homeostasis by cytotoxicity, alterations in oxidative stress, antioxidant response, inflammation, apoptosis and necrosis. Future studies investigating inflammatory pathways, genetic damage, and assays with in vivo and in silico models are important to elucidate the mechanism of the cellular damage caused by exposure to these herbicides. Full article

Pesticides represent a significant threat to aquatic ecosystems due to their persistence and widespread use in agricultural areas, altering environments and exerting adverse effects on non-target organisms. Tebuthiuron is a phenylurea herbicide extensively used as an agrochemical to control pests and weeds in various crops, which often leads to contamination of aquatic environments. Despite its high water solubility and relatively long half-life in soil, studies on Tebuthiuron toxicity in fishes at environmental concentrations are limited. This study aimed to unravel the toxicity mechanisms of Tebuthiuron using the zebrafish model. Zebrafish embryos were exposed to Tebuthiuron (one concentration of either 0.1, 10, 1000 and 5000 µg/L) for 5 days and assessed for hatchability, heart rate, locomotor activity, oxygen reactive species, apoptosis and gene expression. There was no change in frequency of hatch, heart rate, or apoptosis. However, behavioral changes were noted, with hyperactivity in zebrafish larvae during the first light (at 10 µg/L), second light (at 10 and 1000 µg/L) and third dark (10 µg/L) periods of the Visual Motor Response assay. Biochemically, a significant depletion of basal ROS levels was observed at 1000 µg/L. At the molecular level, downregulation of oxidative stress-related genes (cat, sod1, and sod2) in larval fish was noted with exposure to 10 and 5000 µg/L Tebuthiuron, suggesting a depletion of antioxidant enzymes. In addition, some neurotoxicity-related genes were downregulated, such as acetylcholinesterase (ache), while some genes were upregulated, like microtubule-associated protein tau b (maptb) and synapsin 2 alpha (syn2a), with 5000 µg/L Tebuthiuron exposure. In conclusion, Tebuthiuron induces sublethal toxicity characterized by irregular, concentration-specific disruptions to photomotor behavior, basal ROS levels, and transcriptional markers of oxidative stress and synaptic function, even in the absence of acute morphological defects. Future research should prioritize functional assays targeting mitochondrial bioenergetics and antioxidant enzyme activities, alongside evaluations of later developmental stages, to fully elucidate the long-term ecological risks of phenylurea herbicides to non-target aquatic species. Full article

Organophosphorus pesticides (OPs) are persistent environmental contaminants requiring monitoring in aquatic ecosystems. In this study, four novel mineral–carbon composites (K-HNT, K-C-HNT, D-HNT, and D-C-HNT) were synthesized via thermal treatment at 800 °C from halloysite nanotubes, casein, dextrin, and pyrolyzed sawdust. Moreover, these materials were characterized (BET, FT-IR, Raman, SEM/EDS), revealing high specific surface areas (up to 218.3 m²/g for the D-C-HNT composite), and evaluated as solid-phase extraction (SPE) sorbents for the determination of six OPs from water. The eluates were analyzed using GC-MS/MS in MRM mode. This study examined the effect of eluent, sorbent mass, and multiple extractions on SPE efficiency. Ethyl acetate-n-hexane (1:1, v/v) was confirmed to be the most effective eluent, achieving high pesticide recoveries (>80%) at optimal sorbent masses of 50 and 75 mg. The method demonstrated satisfactory sensitivity, with limits of detection (LODs) ranging from 1.6 to 3.3 μgL⁻¹. Notably, the synthesized composites outperformed pure precursors and demonstrated extraction efficiency comparable to commercial sorbents (C-18, Florisil, active carbon). Furthermore, reusability tests confirmed that second and third extraction repetitions reduced the efficiency of the SPE process only slightly, indicating that these waste-derived composites can be reused multiple times without cartridge replacement. Full article

Edible insects are increasingly promoted as alternative foods, but their chemical safety is difficult to assess because hazards may arise throughout the production chain and the regulatory framework remains incomplete. This structured narrative review synthesises current evidence on chemical hazards in edible insects and examines their interpretation within the European Union regulatory framework. The available literature indicates that rearing substrates and production environments are major sources of contamination, particularly for heavy metals, mycotoxins, pesticide residues, veterinary medicinal product residues, and persistent or emerging contaminants. However, transfer into insect biomass is highly species-, life-stage-, and compound-dependent, and metabolism, excretion, or transformation may reduce parent-compound concentrations without eliminating uncertainty regarding metabolites and other transformation products. Processing and storage may further modify the chemical profile through the formation of acrylamide, furan derivatives, lipid-oxidation products, and biogenic amines. Although the EU novel food framework provides product-specific premarket assessment, representative market-occurrence and consumption data remain limited, and generally applicable insect-specific maximum levels for contaminants have not been established, whereas pesticide residues are governed by the horizontal EU MRL framework. Chemical safety assessment should therefore adopt a full-chain, species- and product-specific approach integrating substrate control, contaminant fate, processing, storage, and consumer exposure. Full article

Human fertility is declining across industrialised populations, while the incidence of hormone-dependent reproductive cancers rises. Endocrine-disrupting chemicals (EDCs) and structurally related emerging pollutants are implicated in both. These two outcomes are generally reviewed as separate studies. This review argues that they are two latencies of a single molecular toxicology. The compounds concerned are structurally diverse: phthalates, bisphenols, per- and polyfluoroalkyl substances (PFASs), pesticides, polychlorinated biphenyls (PCBs) and dioxins, brominated and organophosphate flame retardants, pharmaceuticals and personal-care products (PPCPs), and micro- and nanoplastics. They nonetheless converge on a limited repertoire of molecular lesions. These include the disruption of hypothalamic–pituitary–gonadal (HPG) signalling through kisspeptin/GnRH and gonadotropin gene expression and interference at nuclear and membrane hormone receptors (ERα/β, AR, GPER, thyroid receptors, AhR, PPARγ). They also include the inhibition of steroidogenesis at StAR and the CYP11A1–CYP17A1–CYP19A1/3β-HSD/17β-HSD cascade and reactive-oxygen-species generation with mitochondrial dysfunction and Keap1–Nrf2 disruption. Epigenetic reprogramming through DNA methylation, histone modification and non-coding RNAs, together with crosstalk with metabolic and immune signalling, completes the set. These lesions produce measurable cytotoxic and genotoxic damage to gametes and the early embryo: sperm DNA fragmentation and 8-oxo-dG accumulation, blood–testis-barrier breakdown, oocyte meiotic-spindle defects, and granulosa-cell apoptosis and pyroptosis. The same receptor, oxidative and genotoxic hubs drive hormone-dependent reproductive carcinogenesis over longer latencies. The review makes three contributions. First, it traces these shared hubs continuously from fertility impairment to malignancy rather than treating them as separate fields. Second, it grades the certainty of the human evidence class by class, so that robust associations can be distinguished from provisional ones. Third, it integrates pseudo-persistent pollutants alongside the classical persistent compounds. These are micro- and nanoplastics, which act as both toxicants and vectors for adsorbed co-contaminants, and pharmaceutical and personal-care residues sustained by continuous wastewater input. Their inclusion demonstrates that chronic low-dose exposure does not require chemical persistence. We conclude with mitigation strategies and an explicit account of what the current evidence base cannot yet support. Full article

The extensive use of pesticides in modern agriculture has resulted in their persistent accumulation in environmental systems, posing significant risks to ecosystems and human health. Consequently, the development of integrated strategies for the sensitive detection and efficient removal of pesticide residues has become critically important. In this context, fluorescent inorganic–organic hybrid nanoparticles have emerged as versatile platforms owing to their tunable physicochemical properties and distinctive optical behavior. This review provides a comprehensive overview of recent advances in these hybrid nanomaterials for pesticide sensing and remediation. Particular emphasis is placed on the underlying photophysical mechanisms governing detection, including fluorescence quenching, Förster resonance energy transfer (FRET), inner filter effect (IFE), and photoinduced electron transfer (PET). In parallel, the role of interfacial interactions such as hydrogen bonding, electrostatic attraction, and π–π stacking in adsorption processes is critically discussed. Furthermore, these hybrid systems exhibit high adsorption capacities and rapid removal kinetics, enabling efficient pesticide elimination using both adsorption and catalytic degradation pathways. Overall, this review underscores the potential of fluorescent inorganic–organic hybrid nanoparticles as next-generation materials for sustainable environmental monitoring and remediation of pesticide contaminants. Full article

Fluorinated pesticides are an emerging class of environmental contaminants owing to their widespread agricultural use, high persistence, and ability to generate highly stable per- and polyfluoroalkyl substances (PFAS) during environmental degradation. Active ingredients containing fluorinated moieties undergo photolytic, hydrolytic, and microbial transformation, producing persistent metabolites such as trifluoroacetic acid (TFA), TFNA, TFNG, AMTT, and FM-6-1, which are highly mobile substances and may enter the food chain. To address this challenge, complementary LC–MS/MS and GC–MS/MS methods combined with QuEChERS and methanol extraction, and a modified QuPPe-based procedure for TFA and DFA were developed and validated for the determination of 76 fluorinated analytes, including fluorinated pesticides and selected transformation products with diverse physicochemical properties. Validation of the analytical workflow included the assessment of limits of quantification (LOQs), recovery, precision, expanded measurement uncertainty, and matrix effects at three spiking levels. LOQs ranged from 0.002 to 0.050 mg kg⁻¹. Recoveries were 63.9–114.7% for GC–MS/MS and 40.3–109% for LC–MS/MS. The validated analytical workflow is suitable for monitoring fluorinated pesticides and selected transformation products in potatoes, onions, pears, watermelons, carrots, and cauliflower and may support routine food-safety control. Full article

Food adulteration is a critical global threat to public health, economic stability, and social welfare. It primarily occurs in two forms: intentional and incidental. Intentional adulteration, or economically motivated adulteration, involves deliberately adding fraudulent or toxic substances such as hazardous ripening agents and harmful coloring to increase profitability. Substitution of food species is also a widely practiced form. Such adulteration can cause severe acute and chronic health issues, ranging from gastrointestinal distress to cancer and organ failure. Incidental adulteration happens through accidental contamination during processing, storage, or distribution, involving hazards such as heavy metals, mycotoxins, and pesticides. Such contaminants also lead to severe chronic diseases and dangerous allergic reactions. Food adulteration also triggers massive socio-economic consequences. Food safety incidents such as the melamine milk crisis and the horsemeat scandal caused billions of dollars in losses due to healthcare costs, product recalls, damaged brand reputation, and disrupted international trade. Global entities such as the WHO, WTO, and Codex Alimentarius have established robust legislative frameworks. However, effective enforcement remains a significant challenge in many countries. To mitigate such harmful impacts, it is essential to strengthen global surveillance systems, enhance rapid analytical detection methods, and strictly enforce compliance with existing food safety regulations. Full article

Confronted with severe global challenges, including water scarcity, excessive use of chemical fertilizers and pesticides, and heavy metal contamination in soils, conventional agricultural technologies exhibit marked limitations in integrated water–fertilizer management and non-point source pollution control. Leveraging their excellent water retention capacity, intelligent sustained-release properties, and environmental responsiveness, hydrogels offer innovative solutions to advance sustainable agricultural development. This review comprehensively outlines the fundamental types, crosslinking mechanisms, and key functional properties of hydrogels, with a focused discussion on their agricultural deployment as high-efficiency soil conditioners, fertilizer vectors, and pesticide carriers; it deciphers the microscopic water-holding mechanisms under the tristate water model, delineates the divergent water-uptake and retention behaviors between ionic and non-ionic hydrogels, and clarifies the cyclic water-holding and release mechanisms of hydrogels during soil amelioration. Thise paper further synthesizes hydrogel-enabled environmental remediation applications, in which heavy metals and pesticide residues in soils and aquatic systems are removed via functional-group coordination adsorption or photocatalytic degradation; concurrently, hydrogels have been shown to activate plant systemic immunity through calcium-signaling pathways, thereby inducing broad-spectrum antiviral defense responses. Moreover, hydrogels can be integrated into precision agriculture frameworks to enable real-time monitoring of crop physiological status and to support targeted irrigation and fertilization management. This work also evaluates the role of hydrogels in promoting seed germination, root system development, crop metabolic regulation, and stress resilience, while introducing tailored application strategies across distinct plant growth stages. Their documented economic advantages include water conservation, enhanced crop yields, reduced dependence on synthetic fertilizers, and lower labor costs. Nevertheless, the large-scale implementation of hydrogels continues to face multifaceted challenges—particularly poor degradability and latent ecological risks, as conventional polyacrylamide (PAM)-based gels resist soil mineralization and retain potentially neurotoxic monomers, leaving a critical gap in multi-annual field data concerning their non-target interference with native soil aggregate evolution, pore distribution, and rhizospheric carbon–nitrogen footprints. Mechanistically, many hydrogels with tensile strengths below 1 MPa are highly susceptible to three-dimensional network collapse under high-salinity osmotic shock and tillage mechanical stress, exhibiting a precipitous drop in water retention after more than three wet–dry cycles due to deficient long-term structural stability. Compounding these technical gaps are elevated production costs and low farmer adoption, driven by the absence of texture-specific performance thresholds—such as an available water increment ≥ 40% for sandy soils—and the lack of established life-cycle cost models and farmer incentive mechanisms for bio-based hydrogels. Moving forward, hydrogel technology should pivot toward materials innovation and cost-reduction engineering to broaden its applicability, employ ≥3-year, multi-habitat regional trials to delineate ecological benefit–risk boundaries, and ultimately position hydrogels as pivotal enablers of sustainable, green agricultural paradigms. Full article

Atherosclerosis is a complex, multifactorial disease and the leading underlying cause of cardiovascular morbidity and mortality worldwide. Beyond traditional risk factors, growing evidence highlights the critical role of environmental exposures in modulating atherogenesis. In real-world scenarios, individuals are exposed to complex mixtures of contaminants, including particulate matter, toxic metals, pesticides, polycyclic aromatic hydrocarbons, and endocrine-disrupting chemicals. These combined exposures may exert additive, synergistic or antagonistic effects, resulting in biological responses that cannot always be predicted from single-agent exposures. At the cellular and molecular level, both chemical mixtures and environmental co-exposures converge on key pathogenic pathways, including oxidative stress, endothelial dysfunction, chronic inflammation, and lipid dysregulation. These processes may favor monocyte recruitment, foam cell formation, vascular smooth muscle cell remodeling, and plaque instability. Moreover, cumulative exposures are associated with epigenetic alterations, such as dysregulation of DNA methylation and non-coding expression patterns, which may influence long-term susceptibility to atherosclerosis. This structured narrative review synthesizes evidence from 37 studies investigating the cellular and molecular mechanisms underlying the impact of environmental mixtures and multipollutant co-exposures on atherosclerosis. By integrating evidence from epidemiological, toxicological, experimental, bioinformatics and multi-omics studies, while recognizing the predominantly associative nature of the available human evidence, we highlight emerging mechanistic insights and discuss the relevance of mixture-based approaches for improving risk assessment and informing future preventive strategies. Full article

The Island of Montecristo, part of the Tuscan Archipelago, was subjected to an “invasive species” eradication effort co-financed by the European Union under the Life+ “Montecristo 2010” project in 2012. Biocidal agents were utilized in abundance, with the objective of targeting select species of flora and fauna. This included the aerial broadcast of pellets containing brodifacoum to attempt to eradicate the rat population. Brodifacoum’s environmental persistence has led to concerns regarding its potential for secondary, tertiary, and subsequent poisoning of numerous species. This article presents an analysis of probabilistic body mechanics simulations indicating that 4.12 tons of pellets containing brodifacoum would have likely reached the sea following the aerial distribution on Montecristo; a comprehensive literature review of the adverse effects and environmental persistence of second-generation anticoagulant rodenticides (SGARS); as well as a theoretical application of the European Industrial Accident Scale (EIAS) to assess environmental contamination. The question of whether the practice of distributing substantial amounts of pesticides to eradicate a selected species constitutes a conservation initiative or could lead to environmental damage is a salient one, and this article aims to provide a multidisciplinary approach to address this question. Full article

Calcium carbonate (CaCO₃) is a common soil amendment to alleviate cadmium (Cd) transformation and accumulation in brown rice, and tetraconazole is a widely used chiral pesticide to control rice sheath blight. This study investigated the effects of soil amendment CaCO₃ and chiral pesticide tetraconazole on pesticide residues, Cd accumulation, and soil microorganisms in two rice cultivars (ZG 96 with low Cd accumulation and ZY 18 with high Cd accumulation) in Cd-contaminated paddy fields. Results showed that CaCO₃ significantly reduced Cd concentrations in brown rice by 60.0–82.9%. It altered tetraconazole degradation half-lives in rice plants, increased residues in ZG 96 rice husk, and decreased enantioselectivity in soil. Tetraconazole residues in brown rice were lower in ZY 18 than in ZG 96. Both CaCO₃ and tetraconazole reshaped soil microbial diversity, community structure, and metabolic functions, with distinct responses between the two rice cultivars. All residues of tetraconazole and Cd in brown rice were below maximum residue limits. The rice cultivar differences should be considered under the application of amendments and chiral pesticides, and this study would provide valuable data and theoretical basis for their combined treatments. Full article