Search Results (286)

This qualitative study examines how Orthodox and ultra-Orthodox Jewish fathers in Israel describe raising a child with autism within the everyday life of a religious family. Although research on autism and family life has expanded, fathers’ voices remain underrepresented, especially in religious families in which family routines, the Sabbat and holidays, and everyday religious practices shape parenting and participation at home. Data were collected from 127 fathers of children aged 3 to 18 through an online Hebrew questionnaire that included open-ended questions and were analyzed using qualitative content analysis. The findings suggest that religiosity shaped fathers’ caregiving not only as a source of meaning, hope, and emotional strength but also as a practical framework for everyday accommodation and belonging. Four themes emerged: (1) religiosity as an anchor for resilience; (2) paternal love as a religious practice of accommodation; (3) paternal adaptation during the Sabbat and holidays; and (4) religious authority as a basis for legitimizing care practices and preserving the child’s place within family and religious life. Together, these findings underscore the importance of culturally responsive support that acknowledges how religious meaning systems shape paternal care, family participation, and children’s inclusion, while also suggesting that fatherhood may function as an ongoing mediating process within religious family life. Full article

Background/Objectives: Excess weight and dyslipidemia are health conditions growing worldwide in children, including indigenous populations. The concern is their related comorbidities, which could appear at an early age. Given limited information on Seri children, this study aimed to evaluate the prevalence of excess weight and dyslipidemia, and to identify factors associated with BMI-for-age Z-score and dyslipidemia. Methods: This cross-sectional study was conducted among Seri children aged 3–11 years. For prevalence analysis, the BMI-for-age Z-score was calculated as an indicator of excess weight. Dyslipidemia was assessed only in school-age children. Information was collected on potential associated variables, including maternal nutritional status, children’s sleep behaviors, physical activity, diet, and cardiovascular health, as well as household characteristics such as the modernity index and food insecurity. Results: A total of 154 Seri children were evaluated. Among preschoolers, 18.8% were classified at risk of overweight. In school-age children, the combined prevalence of overweight and obesity was 32.8%. Maternal BMI and weight, the modernity index, and being a boy were positively associated with the BMI-for-age Z-score, whereas having food insecurity, cardiovascular health score, and sleep time were negatively associated. Dyslipidemia prevalence was 46.1% among school-age Seri children. Having dyslipidemia was positively associated with maternal BMI, percentage of energy intake from ultra-processed products, paternal occupation as a merchant, and child age, and negatively associated with the number of remunerative maternal economic activities. Conclusions: These findings provide evidence on the prevalence of excess weight and dyslipidemia and their associated factors among Seri children and may inform future research and health strategies in Seri and other vulnerable populations. Full article

Millions of chicks are hatched daily in commercial hatcheries and due to ease of access and the large availability of chicks produced daily, such chicks have been accepted as a reference and control. Unfortunately, this is not a correct assumption. Chickens evolved to be hatched in nests and to remain in close contact with adult hens, which is important for the transfer of chicken-adapted microbiota from hens to offspring. In the absence of adult hens, chicks from hatcheries are colonised by microbiota of environmental origin. Forgetting this fact has led to many confounding conclusions, including a dogma on the age-dependent development of gut microbiota. In this sense, chicks from hatcheries represent a threat. However, if correctly perceived, the same chicks represent a unique opportunity for host–microbiota studies since there is no alternative animal model in which offspring free of any paternal influence are that readily available. Full article

This study investigates the “competence–performance gap” between cognitive ability (measured by the WISC-IV) and actual adaptive performance (measured by the ABAS-II) in children with autism spectrum disorder (ASD), and examines the moderating role of family environment, specifically parental education levels. We applied Latent Profile Analysis (LPA) to cross-sectional data from 3246 children with ASD (aged 6–16 years). The analysis identified three distinct cognitive–adaptive subgroups: the Balanced High-Functioning group (33%), the Classic Mismatch group (44%), and the Cognitively Vulnerable group (23%). Notably, the Classic Mismatch group was characterized by adaptive performance that significantly trailed cognitive potential. Multinomial logistic regression revealed that maternal education—but not paternal education—significantly predicted a child’s likelihood of being in the “Balanced High-Functioning” group. This moderating effect was especially pronounced during the school-age years. These findings highlight the critical role of environmental factors in the translation of intellectual potential into practical social adaptive functioning, providing theoretical support for targeted family-based interventions. Full article

Socioeconomic inequities are associated with longer length of hospital stay (LOS) and clinical severity in children. This retrospective cross-sectional study analyzes health inequalities and area-level deprivation, focusing on hospitalization indicators, in a suburban pediatric population in Portugal. Pediatric admissions to a local general hospital were analyzed for LOS and admission to intensive care unit (ICU), as well as their relation to socioeconomic factors, over an 8-year period (2014 to 2021), using population-averaged models. Area-level inequalities were measured for the population ranked by civil parishes’ European Deprivation Index. 8016 admissions were included. Health inequalities associated with socioeconomic deprivation were observed, with concentration curves above the diagonal for LOS and admission to ICU and located in urban and densely populated civil parishes. Neonatal age showed the highest mean LOS ratio (MR = 2.29, 95% CI 1.96; 2.67, p < 0.001) and ICU admission odds (OR = 9.25, 95% CI 4.84; 17.68, p < 0.001). Mean LOS ratio was significantly higher for Black ethnicity (MR = 1.19; 95% CI 1.10; 1.28, p < 0.001) and lower maternal education. Odds of admission to ICU was significantly higher for male gender (OR = 1.25, 95% CI 1.01; 1.55, p = 0.048) and mother’s unskilled occupation (OR = 1.66, 95% CI 1.09; 2.53, p = 0.019). Paternal manual skilled occupation demonstrated 17% higher mean LOS ratio (p < 0.001) and 51% higher odds of admission to ICU (p = 0.019). Public policies must be culturally competent and target socioeconomic and geographical deprivation. Full article

Due to overfishing, eutrophication of rivers and lakes, and irrational stocking practices, the diversity of wild native carp populations has declined, leading to germplasm degradation and a decrease in fish quality, thereby affecting the sustainable development of fisheries. In this study, a novel hybrid crucian carp lineage (designated LWR) was successfully established via distant hybridization using Dongting Lake crucian carp (LC, ♀) and Hefang crucian carp (WR, ♂) as parental stocks. Systematic analyses were conducted on the morphology, ploidy, fertility, growth performance, survival rate, and molecular genetics of LWR. The results reveal that LWR is an allodiploid (2n = 100), with a chromosome number identical to that of its parents. Gonadal development in the hybrid progeny (LWR) was normal, with both sexes being fertile and reaching sexual maturity at one year of age. Morphologically, LWR exhibited intermediate traits with a paternal bias, characterized by a deep-bodied and elongated shape. In terms of growth performance, LWR displayed significant heterosis (approximately 145% and 271% higher than the body weight of the maternal parent at 6 months and 1 year). Molecular genetic analysis indicated that the 5S rDNA sequences of LWR were predominantly inherited from the paternal parent WR, with insertions, deletions, recombination, and mutations detected. The mtDNA sequences exhibited 99.78% similarity to those of the maternal parent LC, following maternal inheritance with sporadic nucleotide variations. These findings offer a new paradigm for hybridization and a theoretical foundation for the improvement and sustainable utilization of indigenous crucian carp germplasm resources, the selective breeding of improved aquaculture strains, and the sustainable development of fisheries. Full article

Background: Mental health problems often begin in adolescence, yet early detection and intervention remain limited. This study assesses the prevalence of psychological distress and its correlates among high-school students in Makkah, Saudi Arabia, and explores whether specific symptom clusters of depression, anxiety and bipolar/mania can be identified using the General Health Questionnaire-30 (GHQ-30). Methods: A cross-sectional survey was conducted during the 2025–2026 academic year using stratified cluster sampling. A total of 535 students aged 15–18 years completed a questionnaire containing the validated Arabic GHQ-30 and demographic, socioeconomic and lifestyle items. The GHQ-30 was scored with binary 0-0-1-1 scoring (cut-off ≥ 6) to define cases of psychological distress. Item clusters were used to screen for probable depression, anxiety and bipolar/mania. Descriptive statistics characterized the sample. Associations were examined using chi-square tests and multivariable logistic regression. Results: Overall, 70.5% of participants screened positive for psychological distress. The prevalences of probable depression, anxiety and bipolar/mania were 33.1%, 28.2% and 31.2%, respectively. In adjusted models, female gender, insufficient sleep, lack of physical activity and exposure to bullying were associated with increased odds of psychological distress; longer sleep was protective. History of mental health conditions was a strong predictor of probable depression, whereas medication use was protective. Older age and higher paternal education were protective for anxiety. Bullying was the most consistent predictor across all symptom clusters. Conclusions: Psychological distress is highly prevalent among Makkah high-school students. Key determinants include gender, sleep duration, lack of physical activity and bullying. Routine school-based mental health screening, sleep-hygiene education, anti-bullying initiatives and early referral pathways are warranted. Further research should examine and validate GHQ-30 item clusters for specific disorders. Full article

Parent–child sexual communication plays a central role in adolescents’ sexual socialization, yet little research has examined whether frequent communication fosters adolescent disclosure of sexual behaviors and how disclosure relates to sexual debut. This study investigated whether the frequency of parent–child sexual communication is associated with adolescent disclosure and examined relationships between disclosure and age at first intercourse. Data from the Healthy Sexuality Pilot Study included 2044 adolescents (1030 males, 1014 females). Structural equation modeling with multi-group comparison revealed parent-specific pathways: maternal communication frequency was associated with disclosure to mothers (β = 0.66–0.69, p < 0.001), and paternal communication was associated with disclosure to fathers (β = 0.83–0.90, p < 0.001). Cross-parent effects were minimal, suggesting disclosure develops through specific parent–child dyadic communication. Invariance testing supported equivalent model functioning across male and female adolescents. Unexpectedly, greater disclosure to mothers was associated with earlier age at first intercourse for both males (β = −0.08, p < 0.05) and females (β = −0.15, p < 0.05). One possibility is that adolescents who become sexually active subsequently seek parental support, increasing disclosure after debut. Findings indicate that frequent sexual communication is associated with disclosure through parent-specific relational pathways but suggest the disclosure–behavior relationship is more complex than protective models predict. Results underscore the importance of promoting not only communication frequency but also quality and effective parental responses. Full article

Background: Glufosinate ammonium (GLA) is a widely used herbicide, yet potential neurodevelopmental risks related to paternal exposure before conception remain insufficiently defined. Methods: In this study, adult male C57BL/6J mice received GLA at 0.2 mg/kg·day for 10 consecutive weeks and were then mated with unexposed females to generate F1 offspring. Offspring growth was monitored, and neurobehavior was assessed at 5 weeks of age. Results: In behavioral tests, female offspring showed reduced social novelty preference in the three-chamber test and impaired spatial learning and memory in the Morris water maze test, while open field, elevated plus maze, and rotarod performance were not altered. Male offspring showed no clear group differences in these memory-related endpoints. Golgi staining revealed reduced dendritic complexity and spine density in the hippocampus and prefrontal cortex. Glial markers were elevated, and neuronal marker changes showed region-dependent shifts. TUNEL staining indicated increased apoptosis during embryonic development and persistent apoptotic signals in the juvenile prefrontal cortex, accompanied by cytokine imbalance with increased IL-1β and decreased IL-10 in the hippocampus. Conclusion: These results suggest that paternal preconception GLA exposure is associated with selective memory-related behavioral deficits in juvenile offspring and with convergent glial, inflammatory, and apoptosis-related brain changes. These findings support the consideration of paternal exposure in developmental risk assessment frameworks. Full article

Background/Objectives: Bruck syndrome type 2 (BS2) is an ultra-rare autosomal recessive disorder within the osteogenesis imperfecta (OI) spectrum caused by biallelic pathogenic variants in PLOD2, which encodes lysyl hydroxylase 2 (LH2), an enzyme essential for bone-specific collagen cross-linking. Marked clinical heterogeneity complicates diagnosis, particularly in patients with atypical or incomplete presentations. We aimed to further delineate the clinical and molecular spectrum of PLOD2-associated disease. Methods: In this descriptive case series, we performed clinical, radiological, and molecular evaluations of four patients from three unrelated families, including two previously reported siblings. Molecular testing comprised targeted next-generation sequencing or whole-exome sequencing, followed by Sanger sequencing for variant confirmation and familial segregation analysis where feasible. Results: Four PLOD2 variants (NM_182943.3) were identified: homozygous c.1885A > G (p.Thr629Ala) in two siblings; c.8dup (p.(Cys4MetfsTer35)) and c.2222G > A (p.(Gly741Glu)) in one patient; and homozygous c.2027A > C (p.(Tyr676Ser)) in one infant. Three variants were previously unreported. Two missense variants remained classified as variants of uncertain significance, and the phase of the two heterozygous variants detected in one patient could not be established because a paternal sample was unavailable. Clinical severity was variable: age at first fracture ranged from 3 months to 4 years, and cumulative fracture burden ranged from 3 to multiple recurrent fractures. One 10-year-old patient had a severe OI-like phenotype without congenital contractures. Older patients showed additional axial and pelvic involvement, including craniovertebral junction abnormalities and acetabular protrusion. Conclusions: This case series broadens the range of clinical presentations observed in PLOD2-associated disease and indicates that severe bone fragility may occur in the absence of congenital contractures. These findings support inclusion of PLOD2 in the differential diagnosis of patients with unexplained bone fragility and progressive skeletal deformities. Additional well-characterized cases and functional studies are needed to refine genotype–phenotype correlations and clarify the clinical significance of newly identified variants. Full article

Background/Objectives: Congenital heart defects (CHDs) are associated with disruptions in one-carbon metabolism. In a family-based trio design, we investigated whether plasma concentrations of choline, betaine, and folate are associated with CHD severity. Methods: The study included 72 children with CHD, 69 of their mothers and 64 of the fathers. CHD clinical severity was classified according to the European network of population-based registries for the epidemiological surveillance of congenital anomalies (EUROCAT) system and the German PAN study (Prevalence of Congenital Heart Defects in Newborns). Concentrations of choline, betaine, and folates were quantified in plasma and urine samples from a subgroup of the participants. Results: The children [mean (SD) age 3.1 (3.2) years, 59.7% males] presented with varying CHD severities according to EUROCAT (62.5% severe and 37.5% mild) and PAN classifications (45.8% severe, 30.6% moderate and 23.6% mild). The means (SD) of plasma concentrations of choline were 14.0 (10.0) µmol/L in the children, 9.5 (5.1) µmol/L in the mothers and 10.3 (5.4) µmol/L in the fathers. Plasma choline concentrations < 10 µmol/L were observed in 38 mothers (66.7%) and were associated with having a child with severe CHD [adjusted odds ratio (aOR) 3.7; 95% confidence intervals (95%CIs) = 1.1, 12.2] compared to mothers with choline ≥ 10 µmol/L. Lowered plasma choline concentrations were detected in 27 fathers (62.8%) and were also associated with severe CHD (aOR 7.4; 95%CIs = 1.7, 31.5). Child concentrations of choline, betaine and folate and parents’ concentrations of betaine and folate were not associated with disease severity. Conclusions: Lower plasma choline in the parents detectable several years after conception was related to having a child with severe CHD compared to families of children with higher plasma choline. Maternal and paternal choline metabolism may have a role in modulating CHD severity. Etiological studies aiming at the prevention of congenital anomalies should focus on maternal and paternal risk factors in the preconception and early pregnancy. Full article

Background/Objectives: Amelogenesis imperfecta (AI) is a heterogeneous group of rare hereditary conditions mainly affecting the quantity and/or quality of tooth enamel. Its phenotypic expression is diverse, as is the mutational spectrum of the AI-causing genes and mutations. Integrins are cell-surface receptors that mediate adhesion between cells and between cells and the extracellular matrix. Among these, mutations in integrin αvβ6 have been shown to cause AI; however, phenotypic variation exists between the knockout mouse model and human cases, as well as among different human AI families. Methods: We recruited AI families and performed mutational analysis using whole exome sequencing. Results: We identified compound heterozygous ITGB6 mutations in two families. In Family 1, a paternally transmitted nonsense mutation (NM_000888.5: c.1060C>T, p.(Gln354*)) and a maternally transmitted missense mutation (NM_000888.5: c.2312A>G, p.(Asn771Ser)) were identified; in Family 2, a paternal missense mutation (NM_000888.5: c.1693T>C, p.(Cys565Arg)) and a maternal frameshift mutation (NM_000888.5: c.2091delC, p.(Asn698Metfs*13)) were identified, each causing AI in the respective proband. Both probands exhibited generalized hypoplastic and hypomineralized AI, but no other extraoral symptoms. Conclusions: This report will not only expand the known mutational spectrum of the ITGB6 gene but also provide evidence for the genotype–phenotype correlations, thereby improving our understanding of the functional role of ITGB6 during amelogenesis. Full article

Background/Objectives: Uncooperative behavior in pediatric dental settings remains a significant barrier to effective treatment. Factors such as demographics, psychological variables, and family influences may impact children’s behavior, but their relative importance is not fully understood. This study explores the emotional, familial, and demographic factors influencing cooperation among children in dental clinics in Jeddah, Saudi Arabia. Methods: A cross-sectional study was conducted among children aged 6–11 years attending dental clinics in Jeddah, Saudi Arabia. Participants undergoing non-invasive dental procedures were recruited. Behavioral cooperation was assessed using the Frankl Behavior Rating Scale, and dental anxiety was measured using the validated Abeer Children Dental Anxiety Scale (ACDAS). Data on demographic characteristics, child cognitive factors, and parental dental anxiety were collected through structured interviews. Multivariable logistic regression analysis was performed to identify independent determinants of cooperative behavior. Results: A total of 906 children were included in the analysis. Demographic variables, including gender, age, and income, were not significantly associated with child behavior (all p > 0.05). Fear of losing control emerged as the strongest predictor in the model. Children reporting fear of losing control had significantly lower odds of cooperative behavior (AOR = 0.14, 95% CI [0.10–0.22], p < 0.001). Shyness in the clinic was not statistically significant (p = 0.216). Maternal dental anxiety was significantly associated with child behavior, with higher maternal anxiety scores linked to lower odds of cooperative behavior (AOR = 0.96, 95% CI [0.93–0.997], p = 0.032). Paternal dental anxiety was not significantly associated with child behavior (p = 0.701). Conclusions: Fear of losing control and maternal dental anxiety were independently associated with children’s behavioral responses during dental visits. These findings highlight the relevance of children’s perceived control and maternal anxiety in understanding behavioral outcomes in pediatric dental settings. Full article

Background/Objectives: The mediating role of the diverse range of screen-based sedentary behaviors (SBs) remains understudied, particularly at younger ages. The present study examined the direct and indirect effects of parental BMI and education on ultra-processed food (UPF) consumption among preschoolers, testing the potential mediating role of screen time. Methods: The cross-sectional study sample comprised 919 kindergarten children (484 boys, 52.7%), with ages ranging from 2.2 to 6.8 years (mean: 4.7 ± 1.0 years). Screen-based sedentary behaviors (television viewing, smartphone use, tablet use, computer use, and playing electronic games) were measured by proxy-report fulfilled by parents, separately for weekdays and weekends. UPF consumption (drinks/yogurts, packaged/fast foods, and sweet/salty snacks) was assessed via 24 h recall scales. Path analysis mediation models tested direct effects of maternal/paternal BMI and education on UPF intake, and indirect effects through screen time, controlling for child age and sex. Results: Lower parental education and higher parental BMI were associated with increased mobile device use and UPF consumption (r = 0.10–0.28). Screen-based sedentary behaviors mediated the association between maternal BMI and UPF pathways (15–90% of total effects), particularly for sweet and salty snacks (50–90%). Parental education effects were also mediated by screen time (9–23% indirect effects), with paternal education showing stronger protection against packaged/fast foods. Conclusions: Mobile devices and watching television partially mediate intergenerational transmission of obesogenic dietary patterns from parental BMI/education to preschoolers’ UPF consumption. Findings of the current study support family-centered interventions targeting screen-time limits and UPF exposure, mainly at the weekends, to prevent early obesity trajectories. Full article

Background: Genetic causes of growth failure should be suspected in patients born small for gestational age (SGA) who fail to show postnatal catch-up growth, present with severe short stature (SS), and exhibit a poor or absent response to growth hormone (rhGH) therapy. Mutations in the insulin-like growth factor 1 receptor (IGF1R) gene are associated with impaired growth, intrauterine growth restriction (IUGR), low birth weight and/or length, and postnatal SS. Case Description: A 9-year-old boy, born SGA for birth length, was evaluated for severe SS. Common causes of SS were excluded. At 9 years and 7 months of age, his height was 112.6 cm (−3.99 SDS), weight 18 kg (−3.79 SDS), and BMI 14.2 kg/m² (−1.8 SDS); pubertal development was Tanner stage 1. The target height was 158 cm (−2.62 SDS). Bone age was delayed by approximately one year compared with chronological age. Serum IGF-1 levels were within the upper-normal range for age. GH therapy (0.035 mg/kg/day) was initiated due to the lack of catch-up growth in an SGA subject. After three years of treatment, the height gain was only 0.5 SDS. IGF-1 levels showed a transient treatment-related increase, followed by persistent normalization during ongoing therapy. Next-generation sequencing (NGS) analysis identified novel heterozygous paternal nonsense variant in the IGF1R gene: c.3498C>G (p.Tyr1166Ter). At 12 years of age, impaired fasting glucose and reduced glucose tolerance were detected; consequently, it was decided to discontinue rhGH therapy, also in light of the IGF1R mutation and the lack of height recovery. Conclusions: This case underlines the critical role of genetic testing in the evaluation of patients born SGA. The coexistence of SGA status and an IGF1R gene mutation may provide a clear explanation for both the poor response to rhGH therapy and the increased risk of alterations in glucose metabolism. An extensive narrative review of the literature on growth outcomes and glucose metabolism abnormalities during GH treatment in SGA patients carrying IGF1R variants was also performed. Full article