Search Results (3,228)

Soil health is fundamental for food security, climate regulation, and ecosystem resilience, yet global research and development efforts remain uneven and fragmented. To provide a more integrated perspective, this study examines global soil health research and development trends between 1990 and 2025 while proposing a microbiome-based framework for soil health assessment. Using a PRISMA-based methodology, we combined data from international development projects, scientific publications, patent data, and microbiome-related initiatives to evaluate temporal, thematic, and regional patterns in soil research. The results reveal a sustained increase in global soil-related research, with nutrient management and soil degradation remaining dominant topics, while soil microbiome research and carbon sequestration have emerged as the fastest-growing areas, particularly since 2015. However, significant regional disparities persist, with research concentrated in Asia, Europe, and North America. To address the lack of a coherent microbiome-based soil health assessment system, we propose a structured microbial indicator framework based on twelve functional microbial groups, evaluated through culturable abundance, functional gene abundance, and relative abundance. Additionally, we introduce a unified, database-driven microbiome reference framework that interprets soils relative to known types and conditions. Overall, this study highlights the global transition toward biologically driven and system-oriented soil research and provides a conceptual foundation for more standardized, scalable, and ecologically meaningful soil health assessment. Full article

Pipeline valves play a crucial role in oil and gas exploration, production, transportation, and storage, and a systematic understanding of patent technologies in this field can help identify innovation trends and formulate research and development (R&D) strategies. This study collected more than 5000 pipeline-valve-related patents worldwide from 2006 to 2025, including 2292 invention patents, and adopted a progressive patent analytics approach integrating statistical analysis, network analysis, text mining, and high-value invention patent analysis. The results show that innovation activity in this field has remained active over the past two decades, especially since 2016, when the number of patent publications exceeded 300 in almost every year. China, Russia, the United States, South Korea, and Canada are the major sources of patent activity, with Chinese enterprises and universities making important contributions in terms of patent quantity. However, the analysis of high-value invention patents indicates that representative patents from the United States, Canada, and Russia also have a strong influence. Core innovation directions cover multiple pipeline valve applications in oil and gas extraction, transportation, and storage, with valve control systems and mechanical structures constituting the dominant technologies. The ten identified technological themes and their evolution show that technological innovation in this field has gradually expanded from mechanical improvements in traditional valve bodies, sealing components, and pressure relief devices to diversified directions such as wellhead control, intelligentization, and low-carbon development. The analysis of high-value invention patents further confirms this trend, indicating that pipeline valve technology is being reshaped from a relatively mature mechanical technology field into an integrated technological system that combines mechanical reliability, intelligent control, and other dimensions. Full article

Background: While recent literature emphasizes the predictive value of composite inflammatory and nutritional indices for vascular outcomes, this study evaluates the actual predictive capacity of preoperative indices (PNI, GNRI, SII, NLR, PLR) for de novo arteriovenous fistula (AVF) maturation and 1-year primary patency. Methods: We retrospectively analyzed 945 end-stage renal disease patients who underwent strictly radio-cephalic autologous AVF creation. Preoperative indices were calculated from routine parameters. Diagnostic accuracy for predicting 1-year patency loss was assessed using receiver operating characteristic (ROC) curves, and a multivariate logistic regression model was constructed to adjust for baseline anatomical and clinical variables. Targeted subgroup analyses evaluated high-risk populations, including those with diabetes, coronary, and peripheral artery disease. Results: The 1-year primary and secondary patency rates were 73.3% and 93.1%, respectively. In contrast to prevalent reports, no significant differences in preoperative PNI, GNRI, NLR, PLR, or SII scores existed between patients with patent and thrombosed fistulas (p > 0.05). ROC analyses showed no predictive utility (AUC: 0.476–0.518). Crucially, multivariate logistic regression revealed that preoperative arterial (OR: 0.58, p < 0.001) and venous diameters (OR: 0.51, p < 0.001) were the strongest independent predictors of AVF failure, whereas all systemic biomarkers lacked independent predictive significance. Subgroup analyses confirmed these indices failed to predict AVF outcomes even in high-risk settings with severe endothelial dysfunction. Conclusions: Preoperative composite nutritional and inflammatory indices do not independently predict AVF maturation or long-term patency when adjusted for local anatomy. Local anatomical features and hemodynamics heavily dominate vascular outcomes, indicating that systemic biomarkers have limited standalone clinical utility for guiding preoperative vascular access planning. Full article

Background: Meta-analyses suggest an association between patent foramen ovale (PFO) and white-matter hyperintensities (WMH), but pooled effect sizes do not clarify applicability to routine transthoracic echocardiography (TTE) or provide patient-level risk estimates. Objective: The objective of this study was to evaluate the association between TTE-detected PFO and MRI-defined WMH in routine practice and to develop a practice-anchored framework (PAMAP) that translates literature-derived evidence into individualized risk. Methods: We performed a retrospective, single-center, propensity-matched analysis of 149 adults undergoing TTE and brain MRI (37 PFO-positive, 112 controls). The primary endpoint was WMH (Fazekas ≥ 1). PAMAP synthesized 12 studies; 4 eligible studies were pooled using random-effects meta-analysis to derive a locked shunt coefficient (OR 3.65). The locked model used age and shunt (H); embolic context (E), and atrial stress (A) were neutral until refit. Transportability was assessed at the cohort level (expected vs. observed prevalence) and patient level (calibration, discrimination), followed by a minimal prespecified refit. Results: WMH was more frequent in PFO-positive versus control participants (54% vs. 32%). Literature-based expected prevalence approximated observed prevalence, supporting transportability. The locked model showed acceptable performance (calibration intercept 0.106; slope 0.912; Brier 0.188; AUC 0.756). A parsimonious refit improved performance (Brier 0.176; AUC 0.783), with the shunt term remaining significant (OR 2.45, 95% CI 1.23–4.88). Conclusions: PAMAP translates meta-analytic associations into a transportable patient-level risk framework. In routine TTE-defined PFO populations, the WMH association is preserved, suggesting that incidental PFO may mark early subclinical cerebral injury and enabling calibrated, individualized risk assessment. Full article

Drug repurposing is often promoted as a faster, lower-risk alternative to de novo discovery, yet substantial barriers continue to limit successful implementation. We performed a scoping review of articles included in PubMed and ScienceDirect with the aim to identify and categorize challenges and analyze the intersections between them. Our review included 73 articles which revealed scientific, clinical, regulatory, economic, and implementation barriers, with the principal being the clinical translation of generated candidates. Scientific challenges include the necessity for new Phase II/III trials to validate efficacy, safety, and optimal dosing in new therapeutic contexts. Across disease areas, domain-specific barriers include subgroup-dependent responses in oncology, resistance and penetration challenges in anti-infectives, and data scarcity in rare diseases. Computational and AI-assisted approaches face fragmented data, model robustness, and insufficient validation. In addition, off-patent drugs face evidence requirements as rigorous as those for de novo entities, yet lack the market exclusivity incentives required to attract private investment. Additionally, an “institutional bottleneck” hinders academic researchers from bringing findings “on-label” due to a lack of regulatory infrastructure and collaborative frameworks. We conclude that drug repurposing requires a distinct translational paradigm involving multi-stakeholder collaboration and early regulatory engagement to bridge the gap between laboratory discovery and patient access. Full article

Digital transformation is a key driver of green innovation in agribusiness. While the positive impact of digital transformation on firm innovation has been well documented, its multidimensional nature and heterogeneous associations on agribusiness green innovation remain underexplored. This study deconstructs digital transformation into five business dimensions and two structural features, using a sample of 155 Chinese A-share listed agricultural companies from 2011 to 2021. By combining an explainable machine learning framework integrating Bayesian-optimized XGBoost and SHAP, we identify individual and interaction predictive effects of each feature on green innovation measured by green patent applications. The results reveal correlational evidence that governance digitalization is the dominant predictive driver of agricultural green innovation, followed by institutional digitalization. Merely expanding the scope of digital transformation delivers no substantial improvements in green-patent-based innovation outputs. Different digital dimensions present notable heterogeneous nonlinear correlations with distinct threshold characteristics. We further find significant synergistic interaction linkages across digital dimensions, where coordinated multi-dimensional digital development is critical to fully unlocking the green innovation potential of digital transformation. These findings provide insights for agribusiness managers and policymakers to prioritize digital investment and facilitate low-carbon transition. Full article

Waste cooking oils (WCO) are large-scale residual streams from domestic and industrial food processing. Their improper disposal poses severe environmental risks, yet their integration into the oleochemical sector offers a strategic opportunity for the green transition by substituting fossil-based feedstocks. This systematic review provides a comprehensive assessment of WCO valorization as a sustainable precursor for high-value products, specifically biopolymers, bioplasticizers, and biolubricants. The study followed the PRISMA 2020 guidelines, searching PubMed, Scopus, and MDPI databases (up to September 2025). The search strategy utilized combinations of keywords present in the title. Inclusion criteria focused on peer-reviewed chemical and biotechnological conversion pathways published in English within the last decade. Studies addressing biofuel production, patents, and review were excluded. Screening, data extraction, and qualitative risk of bias assessment, centered on experimental reproducibility and reporting transparency, were performed independently by multiple reviewers. From an initial pool of 2637 records, 87 studies met the eligibility criteria. The analysis reveals that polyhydroxyalkanoates (PHAs) represent the most extensively researched pathway, followed by WCO-derived epoxides and innovative biolubricant formulations. While several studies report high conversion yields under optimized conditions, the transition from bench-scale to industrial implementation remains hindered by the heterogeneous composition of WCO and a lack of standardized pre-treatment protocols. WCO valorization shows transformative potential for the circular economy, offering a dual benefit of waste mitigation and sustainable material synthesis. However, future research must address scalability challenges and feedstock variability. This review identifies emerging trends and provides a roadmap for the industrial adoption of WCO-based processes in the framework of clean technologies. Full article

Background/Objectives: People with non-ST-segment elevation myocardial infarction (NSTEMI) with an occluded culprit vessel represent a unique subset of patients; however, their long-term outcomes remain unclear. This study aimed to compare 5-year mortality between NSTEMI patients treated with percutaneous coronary intervention (PCI) based on TIMI flow states in the culprit vessel on presentation (TIMI 0-1 compared to TIMI 2-3). Methods: A retrospective analysis of prospectively collected data of all NSTEMI patients who underwent PCI from 2012 to 2019 at a tertiary cardiac center (The Freeman Hospital, Newcastle-Upon-Tyne, UK) with follow up for 5 years until January 2024. Patients were identified from the database and categorized based on pre-procedural TIMI flow in the culprit vessel. A propensity score was used to pair TIMI 0-1 patients with a matched cohort of TIMI 2-3 patients. The primary outcome was 5-year all-cause mortality. Results: A total of 775 patients with TIMI 0-1 flow were matched with 750 patients who had TIMI 2-3 flow. Patients with TIMI 0-1 flow were more likely to have transient ST elevation (24% vs. 18%, p < 0.001) or Q waves (4% vs. 1%, p < 0.001) compared with patients who had TIMI 2-3 flow. They were also more likely to have moderately to severely impaired left ventricular systolic function compared with patients with TIMI 2-3 flow (21% vs. 16%, p = 0.01). In-hospital mortality (1.2% vs. 1.2%, p = NS), 1-year mortality (5% vs. 6.9%, p = NS), and 5-year mortality (16% vs. 18%, p = 0.34) were not significantly different between the two groups. The use of glycoprotein IIb/IIIa antagonists was associated with lower mortality, HR 0.64 (0.46 to 0.87). Conclusions: NSTEMI patients with occluded culprit vessels who underwent PCI had similar in-hospital and long-term outcomes to patients with patent culprit vessels. The use of glycoprotein IIb/IIIa inhibitors appears to be associated with lower mortality. Full article

The global expansion of highly pathogenic avian influenza A (H5N1), particularly the clade 2.3.4.4b lineage, has renewed urgent concerns about its pandemic potential in the context of its ongoing panzootic expansion and increasing cross-species transmission. Despite decades of preparedness initiatives, critical technological and structural gaps persist, especially in low- and middle-income countries (LMICs), where both vaccine access and sustainable manufacturing capacity remain limited. In this perspective, we examine key lessons from past influenza pandemics and global preparedness strategies, including the Global Action Plan for Influenza Vaccines, highlighting persistent challenges related to sustainable manufacturing capacity and equitable vaccine access. Additionally, we examine the potential of messenger RNA (mRNA) vaccine platforms to address these limitations, given their rapid design, scalable manufacturing, and adaptability to emerging pathogens. Moreover, we examine the role of neuraminidase (NA) as a complementary antigen capable of broadening immune protection and reducing viral transmission. Finally, we describe recent advances in Latin America, focusing on Argentina’s participation in the mRNA Technology Transfer Programme co-led by the World Health Organization (WHO) and the Medicines Patent Pool (MPP), as a model for strengthening regional manufacturing capacity and contributing to global pandemic preparedness. Together, these elements indicate that effective H5N1 pandemic preparedness will require the integration of improved antigen design, flexible mRNA platforms, and sustainable regional manufacturing systems aligned with global procurement strategies. Full article

Corneal endothelial dysfunction is a major cause of corneal blindness worldwide. This is primarily due to the limited regenerative capacity of human corneal endothelial cells (CECs) and the global shortage of donor tissues. Corneal endothelial cell therapy (CECT), which involves injecting cultured CECs into the anterior chamber, has emerged as a promising alternative to conventional transplantation. However, its clinical efficacy remains limited by several factors, including rapid cell loss, non-uniform distribution, and insufficient long-term adhesion following injection. Recent advances in biomaterials and regenerative engineering have led to the development of emerging biomaterial-assisted strategies aimed at addressing these challenges. In this review, we provide a mechanistic and translational overview of next-generation CECT, highlighting a range of biomaterial-assisted strategies aimed at improving cell retention, spatial localization, and long-term adhesion following injection. These emerging approaches aim to mitigate key limitations of conventional cell injection therapy, including variability in cell distribution and retention. However, their effectiveness and translational feasibility remain under active investigation. In addition, we analyze recent global patent trends, regulatory frameworks, and market dynamics to highlight emerging opportunities for innovation and development in this field. Although many of these technologies remain at the preclinical or early translational stage, these approaches may provide a promising direction to improve engraftment efficiency, reduce surgical variability, and enable more scalable, minimally invasive treatment options. This review highlights the potential of biomaterial-assisted CECT as a next-generation regenerative strategy and outlines key challenges that must be overcome for successful clinical translation. Full article

The pet food sector has progressively evolved over the past decade from conventional nutrition toward functionally targeted and sustainability-oriented systems that are increasingly parallel developments in human health. While numerous reviews have examined individual aspects of pet food innovation, an integrated perspective linking scientific research, patent activity, and global market dynamics remains limited. This review addresses this gap by systematically synthesizing peer-reviewed literature, patent landscapes, and product launch data to identify key drivers and bottlenecks shaping contemporary pet food innovation. The analysis highlights a strong concentration of research and patent activity in health-oriented functional formulations, particularly those targeting gastrointestinal health, immune modulation, and age-related conditions, while postbiotics, precision nutrition, and digital tools remain comparatively underdeveloped. Sustainability-driven ingredients and alternative proteins show growing momentum but face persistent challenges related to scalability, regulation, and sensory acceptance. The commercial success of functional pet foods depends on translating scientific findings into stable, manufacturable, and evidence-supported products. Future innovation will therefore be shaped by technologies that connect biological function with process feasibility and market readiness. This review concludes that future progress in pet food innovation will depend on integrated frameworks that align biological efficacy, technological feasibility, and market viability, thereby bridging the gap between scientific advancement and commercial implementation. Full article

Background: Positron emission tomography (PET) is a molecular imaging technique that exploits the β⁺ decay of selected radionuclides to enable non-invasive in vivo investigation of biochemical and physiological processes, including early and subclinical disease alterations. Radiotracers are designed to bind specific molecular targets with high affinity and selectivity. Among the targets to which PET devotes increasing attention are G protein-coupled receptors (GPCRs)—the largest class of transmembrane receptors—which orchestrate a wide spectrum of biological outcomes and are widely implicated in human disease. Objectives: This review analyzes patents published between 2020 and 2025 focusing on GPCR-targeted PET radiotracers, highlighting design strategies, radionuclide selection, and translational perspectives across oncology, central nervous system (CNS) disorders, and inflammatory diseases. Results: Patent activity shows that most GPCR-targeted PET tracers are derived from validated ligands adapted for imaging while preserving affinity and selectivity. Oncology patents mainly favor peptide-based or modular metal–chelator platforms enabling radionuclide flexibility and theranostic extension, whereas CNS tracers rely on drug-like small molecules optimized under strict ADME and blood–brain barrier constraints. Increasing emphasis on non-orthosteric, function-sensitive, and dual-targeting approaches reflects a shift toward interrogating GPCR signaling states, while inflammatory indications remain comparatively underrepresented despite clear biological foundations. Conclusions: Current patent trends consolidate GPCR-targeted PET tracers as well-established diagnostic tools while progressively expanding their clinical utility, both as platforms supporting translational research—informing mechanistic insight and drug development—and as components of emerging theranostic strategies across multiple disease areas. Full article

Mineral exploration in the Iberian Pyrite Belt follows increasingly deeper targets. The present study introduces an innovative methodology for the detection and identification of blind metallic mineral deposits, in particular volcanogenic massive sulfides based on surface rock coatings. This approach follows the identification pathways of upward metal escape routes and metal distribution in rock fractures located in different anisotropic or isotropic planes above the Neves-Corvo VMS deposit ore lenses, using VP-SEM-EDS and XRD. Coatings are dominated by poorly crystalline to amorphous phases, with goethite and birnessite as the main Fe- and Mn-bearing minerals. Copper, zinc and lead are systematically enriched in coatings developed above or near the ore bodies, reflecting chalcopyrite, sphalerite and galena acidic leaching. Tin shows a restricted and heterogeneous distribution, while Ni and Co display no systematic relationship with the ore bodies. Barium and late Ba–Pb–(Zn) mineralization along fault zones record VMS mineralization. Lead isotopic coating signatures overlap those of IPB massive sulfide deposits, confirming a dominant VMS-derived contribution. Fe–Mn coatings were formed by precipitation from ascending meteoric fluids that leached metals from massive sulfides, their alteration halos, and surrounding lithologies, preserving the geochemical footprint of buried mineralization. This approach constitutes a new patented exploration tool. Full article

Bacteriophage-based products are gaining attention as effective tools to reduce harmful germs in food and combat antimicrobial resistance throughout the food production process. However, in South America, their use is still limited because of complicated regulations and inconsistent evidence requirements. This review aims to (i) explore the current scientific and technological landscape of using bacteriophages in South American food systems, (ii) identify main regulatory challenges that impact their classification, approval, and use, and (iii) highlight the need for consistent international guidelines, especially from Codex Alimentarius, to help safely and effectively incorporate phage-based products in food. Research on phage-based products is growing, but it is not consistent across different regions. There are more patents and advancements in biotechnology, but they are limited to certain areas. Although progress is being made, the regulatory frameworks are still unclear, especially when it comes to how these products are classified, labeled, and monitored for safety. To address these gaps, risk-based guidelines are needed. These should define product categories and claims, set safety standards, and include rules for tracking products and monitoring them after they hit the market. Creating a new Codex Alimentarius project on phage-based products could help establish global guidelines that promote safe use, reduce uncertainty in regulations, and improve trade in food markets around the world. Full article

The global push for low-carbon electricity generation has made hydrogen-enriched natural gas an attractive near-term decarbonization option. This paper combines experimental and thermodynamic analyses of H₂–CH₄ combustion in gas turbine combustion chambers. Experiments were conducted on a patented two-stage swirl burner across 240 operating conditions. The effects of hydrogen fraction (γ = 0–40%), swirler vane angle (30°, 45°, 60°), equivalence ratio (φ = 0.17–1.00), and fuel injection strategy were measured against NOx and CO emissions and lean blowout stability. Each 10% increase in hydrogen content raised NOx by 23–24% via the Zel’dovich thermal mechanism, while CO fell by up to 28.5% at φ = 0.3 and 60° due to enhanced OH-radical activity. The minimum recorded NOx was 12.08 ppm (Type 2 injection, 30°, γ = 0%, φ = 0.3). Hydrogen addition improved lean blowout stability by 32–46% per 10% H₂. A parallel thermodynamic analysis showed that integrating an organic Rankine cycle (ORC) and supplementary H₂–CH₄ firing in the heat recovery steam generator cuts specific CO₂ emissions by 7.5–10% and raises net efficiency by 0.79–4.0 percentage points. Critical comparison with 28 published studies identified an optimal operating window: γ = 20–30%, φ = 0.5–0.7, 45° vane angle (SW = 0.8). Full article