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16 pages, 1105 KiB  
Article
Ozone Stress During Rice Growth Impedes Grain-Filling Capacity of Inferior Spikelets but Not That of Superior Spikelets
by Shaowu Hu, Hairong Mu, Yunxia Wang, Liquan Jing, Yulong Wang, Jianye Huang and Lianxin Yang
Agronomy 2025, 15(8), 1809; https://doi.org/10.3390/agronomy15081809 - 26 Jul 2025
Viewed by 215
Abstract
Ozone pollution decreases rice yield and quality in general, but how ozone stress changes grain-filling capacity is unclear. A chamber experiment was conducted to compare the effects of ozone exposure during the rice growth season on the grain-filling capacity and quality of spikelets [...] Read more.
Ozone pollution decreases rice yield and quality in general, but how ozone stress changes grain-filling capacity is unclear. A chamber experiment was conducted to compare the effects of ozone exposure during the rice growth season on the grain-filling capacity and quality of spikelets located on the upper primary rachis (superior spikelets, SS) and the lower secondary rachis (inferior spikelets, IS). Ozone stress significantly decreased filled grain percentage by 41.4% and grain mass by 10.2% in IS, but had little effect on grain-filling capacity in SS. Consistent with the reduction in grain mass, ozone stress decreased grain volume, mainly due to reduced grain thickness, and IS was reduced more than SS. After removing the hull, brown rice obtained from ozone treatment exhibited higher proportions of immature and abnormal kernels, resulting in a substantially lower proportion of perfect kernels. Under ozone stress, the proportion of perfect kernels was only one-third in IS, compared with two-thirds in SS. Ozone stress affected the pasting properties of brown rice for both SS and IS, as shown by the decreased amylose content, and the increased maximum viscosity, minimum viscosity, final viscosity, setback, and peak time of the rapid visco analyzer profile. Out of fourteen traits related to nutritional quality of brown rice, only five showed significant increases under ozone stress, and they were the concentrations of albumin, prolamin, sulfur, copper, and manganese. The differential ozone responses between SS and IS were rather small for rice pasting properties and chemical compositions as shown by very few significant interactions between ozone and grain position. It is concluded that ozone stress during plant growth imposed more adverse effects on IS than SS in terms of grain-filling capacity and appearance quality, suggesting an enlarged asynchronous grain-filling pattern in rice panicles under ozone pollution. Strategies to improve the grain-filling capacity of IS are needed to mitigate ozone-induced damage to rice production. Full article
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14 pages, 1840 KiB  
Article
Population Genetics of the Asian Buffalo Leech (Hirudinaria manillensis) in Southern China Based on Mitochondrial Protein-Coding Genes
by Gonghua Lin, Jingjing Yin, Wenting Zhang, Zuhao Huang, Zichao Liu, Huanhuan Chen, Lizhou Tang and Fang Zhao
Biology 2025, 14(8), 926; https://doi.org/10.3390/biology14080926 - 23 Jul 2025
Viewed by 247
Abstract
Leeches hold significant medical and pharmaceutical value for antithrombotic treatments, yet their genetic diversity patterns remain poorly understood. We performed population genetic analyses on seven Hirudinaria manillensis populations from southern China using mitochondrial protein-coding genes (MitPCGs). Complete sequences of all 13 MitPCGs were [...] Read more.
Leeches hold significant medical and pharmaceutical value for antithrombotic treatments, yet their genetic diversity patterns remain poorly understood. We performed population genetic analyses on seven Hirudinaria manillensis populations from southern China using mitochondrial protein-coding genes (MitPCGs). Complete sequences of all 13 MitPCGs were obtained from 74 individuals. Haplotype diversity exhibited a logarithmic relationship with the gene length (R2 = 0.858, p < 0.001), while nucleotide diversity showed a near-perfect alternating low-high pattern (Z = 2.938, p = 0.003). Concatenated sequence analyses indicated high haplotype diversity (>0.5) and low nucleotide diversity (<0.005) across all populations, suggesting a historical bottleneck followed by rapid expansion and mutation accumulation. The haplotype network, haplotype phylogenetics, and genetic structure analyses revealed moderate genetic differentiation across populations, dividing them into three clades: a basal Yunnan population (YNHH), sub-basal Guangxi populations (GXGG, GXLZ, and GXYL), and distal Guangdong/Hainan populations (GDMM, GDZJ, and HNDA). Analysis of historical population demography revealed five phases from ancient to recent times (P1–5): growth, prolonged stability, rapid decline, rapid growth, and secondary decline. These phases correlate strongly with past climatic events, demonstrating that glacial–interglacial cycles profoundly impacted the leech’s effective population size. This study provides a key scientific basis for H. manillensis resource conservation and utilization. Full article
(This article belongs to the Special Issue Genetic Variability within and between Populations)
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27 pages, 3716 KiB  
Article
Raman Characterization of Dioxygen Species as Defects in Single-Crystal ZnO Including Their Pressure Dependence
by Dieter Fischer, Dominik Bloos, Aleksandra Krajewska, Graham M. McNally, Dejan Zagorac and Johann Christian Schön
Crystals 2025, 15(6), 574; https://doi.org/10.3390/cryst15060574 - 18 Jun 2025
Viewed by 409
Abstract
The defects in zinc oxide crystals are of crucial importance for their usability in many applications and are not yet fully understood. Here, we demonstrate that dioxygen species are present as defects in the grown ZnO, resulting in a bending of the atom [...] Read more.
The defects in zinc oxide crystals are of crucial importance for their usability in many applications and are not yet fully understood. Here, we demonstrate that dioxygen species are present as defects in the grown ZnO, resulting in a bending of the atom layers that lie perpendicular to the c-axis. In the Raman spectra, these defects cause the appearance of bands different from the known bands of perfect ZnO crystals allowed by symmetry. These additional Raman bands, which have been frequently reported for ZnO in the past, can thus be fully explained by the presence of dioxygen species, and the widespread assumption of second-order modes for the assignments of these bands is not necessary. Furthermore, the Raman spectrum belonging to perfect zinc oxide in the ideal wurtzite structure is presented, obtained from small domains in ZnO(0001) crystals exposed to pressures up to 2 GPa. The dependence of the O-O stretching modes on the applied pressure proves the presence of dioxygen species in ZnO, which is also confirmed by phonon calculations of structure models with embedded dioxygen species. The surface quality of the ZnO crystals studied is also reflected in the Raman spectra and is included in the analysis. Full article
(This article belongs to the Section Inorganic Crystalline Materials)
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20 pages, 1143 KiB  
Review
Perfecting Sensory Restoration and the Unmet Need for Personalized Medicine in Cochlear Implant Users: A Narrative Review
by Archana Podury, Brooke Barry, Karen C. Barrett and Nicole T. Jiam
Brain Sci. 2025, 15(5), 479; https://doi.org/10.3390/brainsci15050479 - 1 May 2025
Viewed by 1628
Abstract
Hearing loss is one of the most common and undertreated medical conditions worldwide, with an estimated 466 million people (5% of the world’s population) reporting disabling hearing impairment. The implications are significant; untreated hearing loss increases the risk of depression, social isolation, unemployment, [...] Read more.
Hearing loss is one of the most common and undertreated medical conditions worldwide, with an estimated 466 million people (5% of the world’s population) reporting disabling hearing impairment. The implications are significant; untreated hearing loss increases the risk of depression, social isolation, unemployment, cognitive decline, and falls. Cochlear implants (CIs) are surgically implanted electrical devices that allow people with severe hearing loss to process sound. Over the past 50 years, CI development has made remarkable ground, such that most CI users have adequate speech perception in a silent environment. These language achievements, while significant milestones, fall short of perfect sensory restoration. Many of these limitations with complex sound perception are due to our one-size-fits-all approach towards CIs and speech-based metrics for evaluating implant performance. In the past decade, there has been exponential interest in improving CI-mediated music perception, as it serves as a key conduit to restoring normal hearing. The present literature demonstrates the need for a personalized approach towards cochlear implantation and management. Our proposed narrative review illustrates the limitations of CI-mediated sound processing and discusses ways in which precision medicine can be introduced into the ever-expanding hearing loss population. Full article
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21 pages, 2829 KiB  
Article
Aspectual Variation in Negated Past Tense Contexts Across Slavic
by Dorota Klimek-Jankowska, Alberto Frasson and Piotr Gulgowski
Languages 2025, 10(4), 78; https://doi.org/10.3390/languages10040078 - 8 Apr 2025
Viewed by 635
Abstract
This study examines variation in the use and interpretation of the perfective (pfv) aspect in negated past tense contexts across East Slavic and selected West and Southwest Slavic languages. Unlike West and Southwest Slavic, where the pfv + neg in past [...] Read more.
This study examines variation in the use and interpretation of the perfective (pfv) aspect in negated past tense contexts across East Slavic and selected West and Southwest Slavic languages. Unlike West and Southwest Slavic, where the pfv + neg in past tense contexts allows for an interpretation denying the existence of the event at any past time, East Slavic uniquely interprets the pfv aspect in these contexts as indicating that the agent either planned but failed to realize the event or initiated it but failed to complete it. We account for this by assuming that negation operates either high (¬TP), as sentential negation, or low (¬vP), over the event domain. In East Slavic, the interaction of the pfv aspect with the past tense prevents high negation and enforces low negation, resulting in inhibited event reading. This reading implies that the event was expected or initiated but ultimately unrealized. We argue that the semantics of the pfv aspect in East Slavic parallels the semantics of specific indefinites in the nominal domain. The aspect head introduces a temporal variable t, which, via a choice function, restricts the domain of existential quantification over t to a singleton set, presupposing the existence of t, which cannot be canceled by high negation. Consequently, in negated pfv past tense contexts in East Slavic, negation scopes over the event domain giving rise to special interpretative constraints in past tense perfective contexts with negation. Full article
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17 pages, 2411 KiB  
Article
Modeling and Evaluation of Forecasting Models for Energy Production in Wind and Photovoltaic Systems
by Imene Benrabia and Dirk Söffker
Energies 2025, 18(3), 625; https://doi.org/10.3390/en18030625 - 29 Jan 2025
Cited by 1 | Viewed by 937
Abstract
The comprehensive change from known, classical energy production methods to the increased use of renewable energy requires new methods in the field of efficient application and use of renewable energy. The urban energy supply presents complex challenges in improving efficiency; therefore, the prediction [...] Read more.
The comprehensive change from known, classical energy production methods to the increased use of renewable energy requires new methods in the field of efficient application and use of renewable energy. The urban energy supply presents complex challenges in improving efficiency; therefore, the prediction of the dynamical availability of energy is required. Several approaches have been explored, including statistical models and machine learning using historical data and numerical weather prediction models using mathematical models of the atmosphere and weather conditions. Accurately forecasting renewable energy production involves analyzing factors such as related weather conditions, conversion systems, and their locations, which influence both energy availability and yield. This study focuses on the short-term forecasting of wind and photovoltaic (PV) energy using historical data and machine learning approaches, aiming for accurate 8 h predictions. The goal is to develop models capable of producing accurate short-term forecasts of energy production from both resources (solar and wind), suitable for later use in a model predictive control scheme where generation and demand, as well as storage, must be considered together. Methods include regression trees, support vector regression, and regression neural networks. The main idea in this work is to use past and future information in the model. Inputs for the PV model are past PV generation and future solar irradiance, while the wind model uses past wind generation and future wind speed data. The performance of the model is evaluated over the entire year. Two scenarios are tested: one with perfect future predictions of wind speed and solar irradiance, and another considered realistic situation where perfect future prediction is not possible, and uncertain prediction is accounted for by incorporating noise models. The results of the second scenario were further improved using the output filtering method. This study shows the advantages and disadvantages of different methods, as well as the accuracy that can be expected in principle. The results show that the regression neural network has the best performance in predicting PV and wind generation compared to other methods, with an RMSE of 0.1809 for PV and 5.3154 for wind, and a Pearson coefficient of 0.9455 for PV and 0.9632 for wind. Full article
(This article belongs to the Section B: Energy and Environment)
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33 pages, 3010 KiB  
Review
Towards Point-of-Care Single Biomolecule Detection Using Next Generation Portable Nanoplasmonic Biosensors: A Review
by Saeed Takaloo, Alexander H. Xu, Liena Zaidan, Mehrdad Irannejad and Mustafa Yavuz
Biosensors 2024, 14(12), 593; https://doi.org/10.3390/bios14120593 - 4 Dec 2024
Cited by 4 | Viewed by 2382
Abstract
Over the past few years, nanoplasmonic biosensors have gained widespread interest for early diagnosis of diseases thanks to their simple design, low detection limit down to the biomolecule level, high sensitivity to even small molecules, cost-effectiveness, and potential for miniaturization, to name but [...] Read more.
Over the past few years, nanoplasmonic biosensors have gained widespread interest for early diagnosis of diseases thanks to their simple design, low detection limit down to the biomolecule level, high sensitivity to even small molecules, cost-effectiveness, and potential for miniaturization, to name but a few benefits. These intrinsic natures of the technology make it the perfect solution for compact and portable designs that combine sampling, analysis, and measurement into a miniaturized chip. This review summarizes applications, theoretical modeling, and research on portable nanoplasmonic biosensor designs. In order to develop portable designs, three basic components have been miniaturized: light sources, plasmonic chips, and photodetectors. There are five types of portable designs: portable SPR, miniaturized components, flexible, wearable SERS-based, and microfluidic. The latter design also reduces diffusion times and allows small amounts of samples to be delivered near plasmonic chips. The properties of nanomaterials and nanostructures are also discussed, which have improved biosensor performance metrics. Researchers have also made progress in improving the reproducibility of these biosensors, which is a major obstacle to their commercialization. Furthermore, future trends will focus on enhancing performance metrics, optimizing biorecognition, addressing practical constraints, considering surface chemistry, and employing emerging technologies. In the foreseeable future, these trends will be merged to result in portable nanoplasmonic biosensors offering detection of even a single biomolecule. Full article
(This article belongs to the Special Issue Micro-nano Optic-Based Biosensing Technology and Strategy)
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26 pages, 8947 KiB  
Article
Angle of Attack Characteristics of Full-Active and Semi-Active Flapping Foil Propulsors
by Lei Mei, Wenhui Yan, Junwei Zhou, Yongqi Tang and Weichao Shi
Water 2024, 16(20), 2957; https://doi.org/10.3390/w16202957 - 17 Oct 2024
Viewed by 1020
Abstract
As a propulsor with a good application prospect, the flapping foil has been a hot research topic in the past decade. Although the research results of flapping foils have been very abundant, the performance-influencing mechanism of flapping foils is still not perfect, and [...] Read more.
As a propulsor with a good application prospect, the flapping foil has been a hot research topic in the past decade. Although the research results of flapping foils have been very abundant, the performance-influencing mechanism of flapping foils is still not perfect, and the research considering three-dimensional (3D) effects for engineering applications is still very limited. Based on the above considerations, a systematic and parametric analysis of a small aspect ratio flapping foil is conducted to correlate the influencing factors including angle of attack (AoA) characteristics and wake vortex on the propulsive efficiency. Three-dimensional numerical analyses of full-active and semi-active flapping foils are carried out in this paper, in which the former focuses on different heave amplitudes and pitch amplitudes, and the latter concentrates on different spring stiffnesses. The analysis covers the full range of advance coefficient, which starts around 0 and ends at a thrust drop of 0. Firstly, the influence of the maximum AoA (αmax) on the efficiency and thrust coefficient of these two kinds of flapping foils is analyzed. The results show that for the small aspect ratio flapping foil in this paper, regardless of the full-active or semi-active form, the peak efficiency as high as 75% for both generally appears around αmax = 0.2 rad, while the peak thrust coefficient of 0.5 occurs near αmax = 0.3 rad. Then, by analyzing the wake flow field, it is found that the lower efficiency of larger αmax working points is mainly due to the larger vortex dissipation loss, while the lower efficiency of smaller αmax working points is mainly due to the larger friction loss of the foil surface. Furthermore, the plumpness of different AoA curves is compared and analyzed. It was found that, unlike the results of full-active flapping foils, the shape of the AoA curve of semi-active flapping foils with different spring stiffnesses is similar, and the relationship with efficiency is not strictly corresponding. This study is expected to provide guidance on both academics and industries in relevant fields. Full article
(This article belongs to the Special Issue CFD in Fluid Machinery Design and Optimization)
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23 pages, 7330 KiB  
Article
Entropy-Driven Crystallization of Hard Colloidal Mixtures of Polymers and Monomers
by Olia Bouzid, Daniel Martínez-Fernández, Miguel Herranz and Nikos Ch. Karayiannis
Polymers 2024, 16(16), 2311; https://doi.org/10.3390/polym16162311 - 15 Aug 2024
Cited by 1 | Viewed by 1392
Abstract
The most trivial example of self-assembly is the entropy-driven crystallization of hard spheres. Past works have established the similarities and differences in the phase behavior of monomers and chains made of hard spheres. Inspired by the difference in the melting points of the [...] Read more.
The most trivial example of self-assembly is the entropy-driven crystallization of hard spheres. Past works have established the similarities and differences in the phase behavior of monomers and chains made of hard spheres. Inspired by the difference in the melting points of the pure components, we study, through Monte Carlo simulations, the phase behavior of athermal mixtures composed of fully flexible polymers and individual monomers of uniform size. We analyze how the relative number fraction and the packing density affect crystallization and the established ordered morphologies. As a first result, a more precise determination of the melting point for freely jointed chains of tangent hard spheres is extracted. A synergetic effect is observed in the crystallization leading to synchronous crystallization of the two species. Structural analysis of the resulting ordered morphologies shows perfect mixing and thus no phase separation. Due to the constraints imposed by chain connectivity, the local environment of the individual spheres, as quantified by the Voronoi polyhedron, is systematically more spherical and more symmetric compared to that of spheres belonging to chains. In turn, the local environment of the ordered phase is more symmetric and more spherical compared to that of the initial random packing, demonstrating the entropic origins of the phase transition. In general, increasing the polymer content reduces the degree of crystallinity and increases the melting point to higher volume fractions. According to the present findings, relative concentration is another determining factor in controlling the phase behavior of hard colloidal mixtures based on polymers. Full article
(This article belongs to the Special Issue Computational Modeling and Simulations of Polymers)
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12 pages, 5319 KiB  
Article
A Method for Maintaining a Unique Kurume Kasuri Pattern of Woven Textile Classified by EfficientNet by Means of LightGBM-Based Prediction of Misalignments
by Kohei Arai, Jin Shimazoe and Mariko Oda
Information 2024, 15(8), 434; https://doi.org/10.3390/info15080434 - 26 Jul 2024
Cited by 1 | Viewed by 1134
Abstract
Methods for evaluating the fluctuation of texture patterns that are essentially regular have been proposed in the past, but the best method has not been determined. Here, as an attempt at this, we propose a method that applies AI technology (learning EfficientNet, which [...] Read more.
Methods for evaluating the fluctuation of texture patterns that are essentially regular have been proposed in the past, but the best method has not been determined. Here, as an attempt at this, we propose a method that applies AI technology (learning EfficientNet, which is widely used as a classification problem solving method) to determine when the fluctuation exceeds the tolerable limit and what the acceptable range is. We also apply this to clarify the tolerable limit of fluctuation in the “Kurume Kasuri” pattern, which is unique to the Chikugo region of Japan, and devise a method to evaluate the fluctuation in real time when weaving the Kasuri and keep it within the acceptable range. This study proposes a method for maintaining a unique faded pattern of woven textiles by utilizing EfficientNet for classification, fine-tuned with Optuna, and LightGBM for predicting subtle misalignments. Our experiments show that EfficientNet achieves high performance in classifying the quality of unique faded patterns in woven textiles. Additionally, LightGBM demonstrates near-perfect accuracy in predicting subtle misalignments within the acceptable range for high-quality faded patterns by controlling the weaving thread tension. Consequently, this method effectively maintains the quality of Kurume Kasuri patterns within the desired criteria. Full article
(This article belongs to the Special Issue AI-Based Image Processing and Computer Vision)
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16 pages, 264 KiB  
Article
Islamic Theism and the Multiverse
by Enis Doko
Religions 2024, 15(7), 861; https://doi.org/10.3390/rel15070861 - 17 Jul 2024
Cited by 1 | Viewed by 6607
Abstract
In this paper, we argue that under certain assumptions, Islamic theism moves in the direction of a multiverse. We present several arguments in two major categories. The first is based on the divine attribute of everlastingness: if God’s everlasting attributes are expressed in [...] Read more.
In this paper, we argue that under certain assumptions, Islamic theism moves in the direction of a multiverse. We present several arguments in two major categories. The first is based on the divine attribute of everlastingness: if God’s everlasting attributes are expressed in the creation and the universe has a finite past, then God created a multiverse. The second category involves perfect being theology: if some of God’s attributes express themselves in the creation, and God has every compossible perfection, then we should expect God to create a multiverse. Full article
(This article belongs to the Special Issue Problems in Contemporary Islamic Philosophy of Religion)
17 pages, 3251 KiB  
Article
Bicarbazole-Benzophenone Based Twisted Donor-Acceptor Derivatives as Potential Blue TADF Emitters for OLEDs
by Iram Siddiqui, Prakalp Gautam, Dovydas Blazevicius, Jayachandran Jayakumar, Sushanta Lenka, Daiva Tavgeniene, Ernestas Zaleckas, Saulius Grigalevicius and Jwo-Huei Jou
Molecules 2024, 29(7), 1672; https://doi.org/10.3390/molecules29071672 - 8 Apr 2024
Cited by 4 | Viewed by 2294
Abstract
Over the past few decades, organic light-emitting diodes (OLEDs) find applications in smartphones, televisions, and the automotive sector. However, this technology is still not perfect, and its application for lighting purposes has been slow. For further development of the OLEDs, we designed twisted [...] Read more.
Over the past few decades, organic light-emitting diodes (OLEDs) find applications in smartphones, televisions, and the automotive sector. However, this technology is still not perfect, and its application for lighting purposes has been slow. For further development of the OLEDs, we designed twisted donor-acceptor-type electroactive bipolar derivatives using benzophenone and bicarbazole as building blocks. Derivatives were synthesized through the reaction of 4-fluorobenzophenone with various mono-alkylated 3,3′-bicarbazoles. We have provided a comprehensive structural characterization of these compounds. The new materials are amorphous and exhibit suitable glass transition temperatures ranging from 57 to 102 °C. They also demonstrate high thermal stability, with decomposition temperatures reaching 400 °C. The developed compounds exhibit elevated photoluminescence quantum yields (PLQY) of up to 75.5% and favourable HOMO-LUMO levels, along with suitable triplet-singlet state energy values. Due to their good solubility and suitable film-forming properties, all the compounds were evaluated as blue TADF emitters dispersed in commercial 4,4′-bis(N-carbazolyl)-1,10-biphenyl (CBP) host material and used for the formation of emissive layer of organic light-emitting diodes (OLEDs) in concentration-dependent experiments. Out of these experiments, the OLED with 15 wt% of the emitting derivative 4-(9′-{2-ethylhexyl}-[3,3′]-bicarbazol-9-yl)benzophenone exhibited superior performance. It attained a maximum brightness of 3581 cd/m2, a current efficacy of 5.7 cd/A, a power efficacy of 4.1 lm/W, and an external quantum efficacy of 2.7%. Full article
(This article belongs to the Special Issue Novel Functional Materials: Design, Modeling and Characterization)
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25 pages, 692 KiB  
Article
Early Chan Buddhism: A Meditation Movement or New Ways of Writing about Final Authority in Tang China?
by Alan Robert Cole
Religions 2024, 15(4), 403; https://doi.org/10.3390/rel15040403 - 26 Mar 2024
Viewed by 3431
Abstract
This essay argues that the long-standing assumption that Chan Buddhism began as a meditation movement is outdated and needs to be replaced by a paradigm that sees the origins of Chan in a set of literary inventions that took form in the mid-Tang [...] Read more.
This essay argues that the long-standing assumption that Chan Buddhism began as a meditation movement is outdated and needs to be replaced by a paradigm that sees the origins of Chan in a set of literary inventions that took form in the mid-Tang era and were designed to prove that the totality of tradition was owned by certain masters of the day. These bold claims to own perfect tradition were bolstered by newly invented genealogies that worked to show that this or that master was, in effect, a descendant of the Indian Buddha, and, thus, a quasi-Buddha himself. Further finessing these efforts to take over final authority in the world of Tang Buddhism was the studied use of Daoist tropes to naturalize and soften these aggressive claims, all in order to make them more appealing to elite readers who could now be impressed by decidedly Chinese-looking portrayals of perfect Buddhism, set on the timeless ground of the Great Dao, where there could be no competition, envy, literary pretensions, or even Buddhist practices—just pure and total truth in the body of a Chinese man. In trying to make sense of this cycle of carefully rewriting the past in order to control the present (and future), it should be clear that we need to switch to a paradigm that accepts that the seductive reinvention of tradition was done consciously and with no small amount of craft and cunning. Full article
19 pages, 2898 KiB  
Review
High Hopes for the Biofabrication of Articular Cartilage—What Lies beyond the Horizon of Tissue Engineering and 3D Bioprinting?
by Yordan Sbirkov, Murad Redzheb, Nico Forraz, Colin McGuckin and Victoria Sarafian
Biomedicines 2024, 12(3), 665; https://doi.org/10.3390/biomedicines12030665 - 15 Mar 2024
Cited by 3 | Viewed by 2997
Abstract
Technologies and biomaterials for 3D bioprinting have been developing extremely quickly in the past decade as they hold great potential in tissue engineering. This, together with the possibility to differentiate stem cells of different origin into any cell type, raises the hopes in [...] Read more.
Technologies and biomaterials for 3D bioprinting have been developing extremely quickly in the past decade as they hold great potential in tissue engineering. This, together with the possibility to differentiate stem cells of different origin into any cell type, raises the hopes in regenerative medicine once again after the initial breakthrough with stem cells in the 1980s. Nevertheless, three decades of 3D bioprinting experiments have shown that the production of functional tissues would take a longer time than anticipated. Cartilage, one of the simplest tissues in the body, consists of only one cell type. It is not vascularised and innervated and does not have lymphatic vessels either, which makes it a perfect target tissue for successful implantation. The tremendous amount of work since the beginning of this century, combining the efforts of bioengineers, material scientists, biologists, and physicians, has culminated in multiple proof-of-concept constructs that have been implanted in animals. However, there is no single reproducible, standardised, widely accessible and accepted strategy that can be readily applied in the clinic. In this review, we focus on the current progress in the field of the 3D biofabrication of articular cartilage and critically assess failures and future challenges. Full article
(This article belongs to the Special Issue Tissue Engineering and Cell Therapy for Cartilage Repair)
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27 pages, 1074 KiB  
Article
(Re-)invented Chan Lineage, Unique Vietnamese Meditation School, or Both? Thích Thanh Từ’s “Revived” Trúc Lâm Tradition of Thiền Tông
by Trang T. D. Nguyen
Religions 2024, 15(3), 352; https://doi.org/10.3390/rel15030352 - 14 Mar 2024
Viewed by 4315
Abstract
This study explores how images of the past have been deployed to set up current arrangements of leadership and institutional identity by considering the career and teachings of Thích Thanh Từ in connection with his “revived” Buddhist tradition in Vietnam. Promoted as a [...] Read more.
This study explores how images of the past have been deployed to set up current arrangements of leadership and institutional identity by considering the career and teachings of Thích Thanh Từ in connection with his “revived” Buddhist tradition in Vietnam. Promoted as a continuation of the unique and pure Vietnamese Buddhist meditation tradition and associated with the Vietnamese national identity, the contemporary Trúc Lâm (Bamboo Grove) is a pride of many Vietnamese Buddhists. The original Trúc Lâm is claimed to be founded by the heroic King-turned-monk Trần Nhân Tông in the thirteenth century. The tradition was supposedly transmitted through the next two generations and died out. In the twentieth century, a Southern Vietnamese monk, Thích Thanh Từ (1924–), who had quit Pure Land (Tịnh Độ, C. Jingtu 淨土) Buddhism to self-learn and practice meditation, decided to reinvent the medieval Trúc Lâm tradition and became the founder of the contemporary Trúc Lâm. Despite growing up during French colonization and American war, Thanh Từ was not politically involved; instead, he focused on setting up new monasteries, taught meditation, and discouraged his followers from political and social engagement. This paper examines how successful Thích Thanh Từ and his disciples are in popularizing Trúc Lâm in Vietnam, given that the majority of Vietnamese Buddhists follow Pure Land devotional practices. More importantly, it describes how Thích Thanh Từ combines the teachings attributed to Trần Nhân Tông and two Chinese Chan masters, Huike 慧可 (the Second Patriarch) and Huineng 惠能 (the Sixth Patriarch), to form Trúc Lâm’s philosophical views and meditation techniques. With the clear-cut distinction between the delusional mind of sentient beings and the perfect mind of enlightened beings, Thích Thanh Từ presents the goal of Trúc Lâm practice as attaining the state of no-thought and sharpening it to perfection to perceive the “buddha nature” (phật tính, S. buddhadhātu, C. foxing 佛性) understood as the pure mind of nonduality and nonform. Outlining that process, he emphasizes the importance of “sudden awakening” (đốn ngộ, C. dunwu 頓悟) followed by “gradual cultivation” (tiệm tu, C. jianxiu 漸修). His meditation manual for ordinary practitioners with no experience of sudden awakening contains key techniques of (1) stabilizing the mind by counting and then observing breaths, (2) recognizing the “true mind” (chân tâm, C. zhenxin 真心) through practicing “no abiding in thoughts” (biết vọng không theo), “no mind for the externals” (đối cảnh vô tâm), “no dualistic discrimination” (không kẹt hai bên), and then proceeding to the stage of permanently abiding in the nature of true mind. These meditation methods are pertinent to Trúc Lâm’s view that all phenomena that emerge via speculative thoughts are unreal and illusory, and that only the true mind is real. The first section of this paper explores historical connections between Vietnamese and Chinese forms of Buddhism, shedding light on why Trúc Lâm embraces Thiền Tông, which is transmitted from Chinese Chan zong, and how Thích Thanh Từ builds connections between Thiền Tông and the Vietnamese national identity. The second section focuses on Thích Thanh Từ’s own life story, on how he practiced meditation and suddenly experienced “unlearned wisdom” (trí vô sư/vô sư trí, C. wushi zhi 無師智, an alternative term for true mind and buddha nature as a result of his practice) and how he succeeded in spreading the “revived” Trúc Lâm. With the first two sections as a background, in the third section, this paper explores Thích Thanh Từ’s views and practices and critically analyzes those views and practices in the conclusion. Overall, I argue that Thích Thanh Từ’s instructions on meditation are closely intertwined with his view of reality, which in turn is based on the mainstream Chan zong ideas. Full article
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