Search Results (67)

Tetragonia tetragonioides, or New Zealand spinach, is a widespread halophyte native to eastern Asia, Australia, and New Zealand, and naturalized in some Mediterranean regions. This underutilized vegetable is consumed for its leaves, raw or cooked. For the first time, we investigated the feasibility of using whole baby plants (including stems and leaves) as raw material for ready-to-eat (RTE) vegetable production. Our study assessed Tetragonia’s suitability for hydroponic cultivation over two cycles (autumn–winter and spring). We investigated the impact of increasing nutrient rates (only water, half-strength, and full-strength nutrient solutions) and plant densities (365, 497, and 615 plants m⁻² in the first trial and 615 and 947 plants m⁻² in the second) on baby plant production. We also analyzed the plants’ morphological and biochemical characteristics, and their viability for cold storage (21 days at 4 °C) as a minimally processed product. Tetragonia adapted well to hydroponic cultivation across both growing periods. Nevertheless, climatic conditions, plant density, and nutrient supply significantly influenced plant growth, yield, nutritional quality, and post-harvest storage. The highest plant density combined with the full-strength nutrient solution resulted in the highest yield, especially during spring (1.8 kg m⁻²), and favorable nutritional characteristics (β-carotene, Vitamin C, Fe, Cu, Mn, and Zn). Furthermore, Tetragonia baby plants proved suitable for minimal processing, maintaining good quality retention for a minimum of 14 days, thus resulting in a viable option for the RTE vegetable market. Full article

Microalgal biomass offers a promising biofertilizer option due to its nutrient-rich composition, adaptability, and environmental benefits. This study evaluated the potential of microalgal-based biofertilizers—microalgal Chlorella biomass, de-oiled microalgal biomass (DMB), and de-oiled and de-aqueous extract microalgal biomass (DAEMB)—in enhancing lettuce growth, soil nutrient dynamics, and microbial community composition. Lettuce seedlings were cultivated with these biofertilizers, and plant growth parameters, photosynthetic pigments, and nitrogen uptake were assessed. Soil incubation experiments further examined nutrient mineralization rates, while DNA sequencing analyzed shifts in rhizosphere microbial communities. Lettuce grown with these biofertilizers exhibited improved growth parameters compared to controls, with Chlorella biomass achieving a 31.89% increase in shoot length, 27.98% in root length, and a 47.33% increase in fresh weight. Chlorophyll a and total chlorophyll levels increased significantly in all treatments, with the highest concentrations observed in the Chlorella biomass treatment. Soil mineralization studies revealed that DMB and DAEMB provided a gradual nitrogen release, while Chlorella biomass exhibited a rapid nutrient supply. Microbial community analyses revealed shifts in bacterial and fungal diversity, with increased abundance of nitrogen-fixing and nutrient-cycling taxa. Notably, fungal diversity was enriched in biomass and DAEMB treatments, enhancing soil health and reducing pathogenic fungi. These findings highlight microalgal biofertilizers’ potential to enhance soil fertility, plant health, and sustainable resource use in agriculture. Full article

Objective: This study aimed to evaluate the acceptability of a specific menu preparation based on minimally processed foods and the factors associated with the consumption of school meals by students in Brazilian public schools. Methods: A hedonic scale was employed to assess the acceptability of a milk preparation with 100% cocoa and sugar. A structured questionnaire was used to evaluate school food consumption, addressing consumption frequency, dietary habits, environmental conditions, and consumption of competing foods. A binomial logistic regression model was conducted to verify the association between individual variables and conditions of meal supply and consumption. Data analyses were performed using the Jamovi 2022 software. Results: A total of 1080 students participated, with 630 taking part in the acceptability test, and 450 completing the consumption questionnaire. The study highlighted acceptance below the minimum PNAE standards (79.68%) for the tested preparation, indicating a need for further investigation into student preferences. Pleasant taste was the most frequently cited reason (53.2%) for consumption. Disliking the served lunch increased the likelihood of not consuming school meals by 2.73% (p = 0.03). Bringing snacks from home showed no significant impact on consumption (p = 0.677). Using inadequate cutlery increased the likelihood of not consuming school meals by 6.44% (p = 0.009). Conclusions: The study underscored the low acceptance of milk prepared with 100% cocoa and sugar, along with irregular school meal consumption, emphasizing the need for strategies to align students’ taste preferences with healthier menu options. While PNAE ensures nutrient-rich meals, improving flavor, variety, and dining conditions, alongside permanent food and nutrition education, is essential to enhance adherence and support students’ health. Although snacks do not affect the consumption of school meals, controlling snack intake is important from a health perspective. Full article

Background/Objectives: Improved global data allow for a new understanding of what impact the food we produce, eat and dispose of has on the environment, human health and Nature’s resources. The overall goal is to guide decision-makers and individuals by providing in-depth knowledge about the effects of their dietary preferences on human and environmental health. Methods: The method is to investigate ways to reduce environmental degradation and to secure healthy food supplies in an urbanizing world, and to quantify the options. Results: Reviewed articles show that by eating less meat-based food and more plant-based and soilless food, as well as reducing food waste and recycling urban-disposed nutrients as fertilizers, we could reduce agriculture’s land requirement by 50% to 70% while still securing a healthy food supply. Less land under cultivation and pasture would reduce global emissions to air and water to a similar extent, and allow Nature to reclaim freed areas in order to catch more carbon and rejuvenate biodiversity. Thus, we could avoid further environmental degradation such as the current clearing of new fields needed under a business-as-usual regime. Presently, some 17 million people die each year due to poor diets, which is more than double the 7 million deaths since the onset of the COVID-19 pandemic. A return to more plant-based diets with unchanged intake of proteins but less calories, sugar, salt and fat combined with less red meat and ultra-processed food would reduce foremost non-communicable diseases by up to 20% and prolong life. The article suggests that the international focus has gradually turned to the food sector’s big contribution to climate change, biodiversity loss and harmful chemicals as well as to poor human health. It argues that this century’s rapid population growth and urbanization give urban residents a pivotal role in food’s impact on agricultural areas, which today cover half of the globe’s inhabitable areas. Their food demand, rather than the activities of farmers, fishermen or loggers, will guide remedial measures to be taken by individuals, industry and the public sector. A tool to calculate the potential environmental footprints of individual or societal measures is presented. Conclusions: Measures to make the agrifood sector more sustainable are still pending full recognition in international fora such as the UN COP Summits. Smart cities fitted with infrastructures to recycle macro- and micro-nutrients and organic matter have the potential to ameliorate human-induced impacts such as emissions to air and water bodies, crossing planetary boundaries, and polluting extraction of N (nitrogen), P (phosphorus) and K (potassium). Rapid results are within reach since dietary change and the turn-around time of nutrients in food is short compared to decades or centuries for recycled materials in cars or buildings. Full article

Introduction: Reconstructing long bone defects in the upper limbs, particularly the radius and ulna, poses significant challenges. These defects, resulting from trauma, tumors, infections, or congenital anomalies, require precise surgical intervention for functional restoration. Traditional non-vascularized autogenous bone grafts have limitations, such as resorption and limited biological activity. To address these challenges, free vascularized fibular grafts (FVFGs) have been developed, offering enhanced recovery by supplying nutrients and structural support, particularly in large defects or compromised vascularity. Materials and Methods: This retrospective study reviewed patients with significant forearm skeletal defects treated with FVFGs at our institution from January 2008 to January 2019. Included were patients with radius or ulna defects exceeding 8 cm due to trauma, tumor excision, or non-union fractures. Data on demographics, clinical details, surgical techniques, and outcomes—including graft union time, complications, range of motion, and the disabilities of the arm, shoulder and hand (DASH) scores—were analyzed. Results: Eight patients, with a mean age of 27.6 years and an average defect length of 9.8 cm, were included. All patients achieved graft union within an average of 4 months, with no tumor recurrence or significant complications. Functional outcomes showed mean forearm pronation of 56.9 degrees, supination of 52.5 degrees, and a mean DASH score of 17.7. Conclusions: FVFG is a safe and effective technique for managing complex forearm bone defects, providing high union rates and good functional outcomes. It should be considered a primary option for large forearm skeletal defects. Full article

Context: The primary factor in determining whether or not a crop can be produced on a certain type of soil is soil fertility. When faced with many options, farmers frequently find it difficult to decide which crop to plant. We created this project to address that particular issue. The provision of soil data is mandatory since it will significantly influence the determination of the soil’s fertility. The output and accuracy of the model may suffer if the data are not supplied discretely. The nature of the dataset indicates that the result is a binary value, i.e., either “Fertile” or “Non-Fertile”, along with the accuracy percentage of each algorithm. Objective: The main aim of this paper is to determine whether the soil is fertile based on soil properties like N, P, K, Ph, nutrient level, moisture levels, temp, rainfall, and topography. Material/Method: We used the dataset from Kaggle, where N, P, K, and pH values are input into the model, and the ML determines whether it is fertile or not. In this article, four machine learning classifiers are trained, and determine the best classifier based on the performance metrics. Result: The results demonstrated that the machine learning classifier significantly improves prediction accuracy. We used LR, KNN, NB, and DT classifiers to increase the accuracy, as well as to increase the efficiency of the soil fertility assessment. The DT classifier exhibited well in comparison to other classifiers. The DT classifier’s accuracy was 89%, but the performance metrics precision, LR, and KNN, was 90%. Full article

Salivary gland biofabrication represents a promising avenue in regenerative medicine, aiming to address the challenges of salivary gland dysfunction caused by various factors such as autoimmune diseases and radiotherapy. This review examines the current state of bioprinting technology, biomaterials, and tissue engineering strategies in the context of creating functional, implantable salivary gland constructs. Key considerations include achieving vascularization for proper nutrient supply, maintaining cell viability and functionality during printing, and promoting tissue maturation and integration with surrounding tissues. Despite the existing challenges, recent advancements offer significant potential for the development of personalized therapeutic options to treat salivary gland disorders. Continued research and innovation in this field hold the potential to revolutionize the management of salivary gland conditions, improving patient outcomes and quality of life. This systematic review covers publications from 2018 to April 2024 and was conducted on four databases: Google Scholar, PubMed, EBSCOhost, and Web of Science. The key features necessary for the successful creation, implantation and functioning of bioprinted salivary glands are addressed. Full article

Brain diseases are oftentimes life-threatening and difficult to treat. The local administration of drug substances using brain implants can increase on-site concentrations and decrease systemic side effects. However, the biocompatibility of potential brain implant materials needs to be evaluated carefully as implants can trigger foreign body reactions, particularly by increasing the microglia and astrocyte reactivity. To date, these tests have been frequently conducted in very simple in vitro models, in particular not respecting the key players in glial cell reactions and the challenges of surgical implantation characterized by the disruption of oxygen and nutrient supply. Thus, we established an in vitro model in which we treated human glial cell lines with reduced oxygen and glucose levels. The model displayed cytokine and reactive oxygen species release from reactive microglia and an increase in a marker of reactive astrocytes, galectin-3. Moreover, the treatment caused changes in the cell survival and triggered the production of hypoxia-inducible factor 1α. In this comprehensive platform, we demonstrated the protective effect of the natural polyphenol resveratrol as a model substance, which might be included in brain implants to ease the undesired glial cell response. Overall, a glial-cell-based in vitro model of the initial challenges of local brain disease treatment may prove useful for investigating new therapy options. Full article

This study investigated the effects of nonpoint source (NPS) pollution reduction and pollutant dynamics in a highland agricultural watershed in Korea. We employed the SWAT model to simulate hydrological processes and pollution transport within the watershed. The model incorporates future climatic scenarios derived from downscaled climate projections to assess their impacts on NPS pollution and pollutant reduction methods. These changes lead to heightened surface runoff and erosion rates, resulting in elevated sediment and nutrient concentrations. The projection indicates an anticipated increase in the annual average temperature by 1.3 to 2.1 °C by the mid-century, under scenarios SSP126 and SSP585. Additionally, precipitation levels are projected to increase by 31 to 61 mm from the baseline to the end of the century. Variations in hydrological components such as evapotranspiration, streamflow, and soil moisture are expected to range from +3.2 to +17.2%, −9.1 to +8.1%, and 0.1 to 0.7%, respectively, during the years 2040 and 2080. Fluctuations in TN, SS, and TP loading are estimated to range from −4.5 to +2.3%, −5.8 to +29.0%, and +3.7 to +17.4%, respectively. This study emphasizes the importance of adaptive management options for stakeholders and the need for adaptive management options to reduce nonpoint source pollution and protect water quality to maintain sustainable water supplies and conserve the environment in this watershed. Full article

Autologous fat grafting is the gold standard for treatment in patients with soft-tissue defects. However, the technique has a major limitation of unpredictable fat resorption due to insufficient blood supply in the initial phase after transplantation. To overcome this problem, we investigated the capability of a medical-grade poly L-lactide-co-poly ε-caprolactone (PLCL) scaffold to support adipose tissue and vascular regeneration. Deploying FDM 3D-printing, we produced a bioresorbable porous scaffold with interconnected pore networks to facilitate nutrient and oxygen diffusion. The compressive modulus of printed scaffold mimicked the mechanical properties of native adipose tissue. In vitro assays demonstrated that PLCL scaffolds or their degradation products supported differentiation of preadipocytes into viable mature adipocytes under appropriate induction. Interestingly, the chorioallantoic membrane assay revealed vascular invasion inside the porous scaffold, which represented a guiding structure for ingrowing blood vessels. Then, lipoaspirate-seeded scaffolds were transplanted subcutaneously into the dorsal region of immunocompetent rats (n = 16) for 1 or 2 months. The volume of adipose tissue was maintained inside the scaffold over time. Histomorphometric evaluation discovered small- and normal-sized perilipin⁺ adipocytes (no hypertrophy) classically organized into lobular structures inside the scaffold. Adipose tissue was surrounded by discrete layers of fibrous connective tissue associated with CD68⁺ macrophage patches around the scaffold filaments. Adipocyte viability, assessed via TUNEL staining, was sustained by the presence of a high number of CD31-positive vessels inside the scaffold, confirming the CAM results. Overall, our study provides proof that 3D-printed PLCL scaffolds can be used to improve fat graft volume preservation and vascularization, paving the way for new therapeutic options for soft-tissue defects. Full article

Background: Front-of-package nutrition labelling (FOPNL) is an important public health tool for promoting healthier food choices. Therefore, the European Commission has committed to proposing harmonised mandatory FOPNL in Europe. A relevant option for this harmonisation is Nutri-Score (NS), which, however, has been subject to some criticism about its alignment with nutritional recommendations. As a result, the Scientific Committee of the NS published two reports in 2022 and 2023, updating the NS algorithm. The aim of our study was to exploit differences between previous (NS2021) and updated (NS2023) algorithm, using foods from Slovenian food supply. Methods: A total of 19,510 branded foods/drinks from the 2020 Slovenian food supply database were profiled using NS2021 and NS2023. We focused on comparing the distribution of each grade and the discriminatory ability between NS2021 and NS2023, while identifying products that were most affected by the NS2023 changes. We also examined changes in alignment with Slovenian nutritional recommendations based on nationally adapted WHO Europe nutrient profile (WHOE). Results: The results show that both NS2021 and NS2023 have good discriminatory ability, with NS2023 being slightly better in 12 sub-categories. Overall, NS2023 was stricter, with E being the most common grade (32%), whereas NS2021 predominantly assigned a grade of D (28%). While the overall proportion of products with grade C remained almost unaffected, there was a notable decrease in “healthier” products graded A or B, from 30% (NS2021) to 23% (NS2023). NS2023 was stricter than NS2021 in almost all main categories, except for beverages and eggs. Alignment with the WHOE profile was moderate (κ = 0.59) for NS2021 and strong (κ = 0.65) for NS2023. Alignment was improved especially for edible oils and emulsions, fruits and vegetables, and snack foods. Discussion: NS2023 was shown to be stricter and more aligned with recommendations than NS2021. The updated NS2023 addressed limitations such as better grading of cooking oils (especially olive oil), higher penalisation of high sugar and salt content, lower grading of beverages with non-nutritive sweeteners, and slight modifications for nuts and cheeses. This study gives first insights into how the update of the NS algorithm works on real-life data and can support policymakers in the implementation of harmonised FOPNL in Europe. Full article

In Spain and other Mediterranean countries, significant quantities of semi-solid olive mill waste are generated, which should be preferentially applied to agricultural soils to close nutrient cycles. However, two-phase olive mill waste (termed alperujo in Spanish) is known to pose risks to soil quality and plant production when applied to soil in large quantities. Alperujo has high contents of polyphenol substances, which can inhibit microbial growth and are also phytotoxic in nature. However, when applied in appropriate quantities and following specific methods, it is possible that the practice may not pose any risks, and this requires evaluation. As a waste management option, direct application of alperujo can supply plant nutrients and organic matter to degradation-prone Mediterranean soils. In order to validate this circular economy fertilization and soil protection strategy, an 18-month field experiment was undertaken, applying moderate quantities of alperujo on permanent crop groves throughout the Spanish region of Valencia. Eleven experimental parcels with permanent crops managed by farmers were identified to test two scenarios: a single application of 10 t/ha, and a second application of 10 t/ha after 10 months. Soil chemical parameters were assessed at 0, 6, 10, and 18 months. Soil organic carbon, total Kjeldahl nitrogen, pH, electrical conductivity, nitrates, and polyphenol contents were modified by alperujo application, but these effects were highly transient in nature, with generally no lasting effects after 4–6 months for either application scenario. Also, qualitative evaluations carried out by farmers revealed few effects, although some reductions in erosive processes and improvements in plant vigor were noted. As such, based on the measured parameters, it is concluded that the direct soil application of alperujo olive mill wastes at low application rates did not lead to any lasting detrimental effects on soil quality or compromise the productivity of permanent crops in this Mediterranean region. Full article

Access to and the use of irrigation water of adequate quality and targeted nutrient supplementation have become more widespread in recent years. Both crop quality and the irrigation system lifetime are affected by the quality of the water used for irrigation. Micro-irrigation (e.g., drip and sprinkle) is becoming increasingly common alongside the more typical irrigation methods, but it requires expertise and pre-treatment to ensure a proper water supply. The most significant problem is clogging, which can greatly reduce irrigation efficiency. Treatment for irrigation purposes mainly depends on the contaminants that are present in the water. The main treatment options available are biological, electromagnetic and electrostatic treatments, but these have a wide range of effectiveness levels compared to membrane separation technologies. In addition, adsorption treatments are also available, which, depending on the adsorbent used, can greatly improve the pre-treatment of irrigation water. This work provides an overview of adsorbents suitable for the treatment of irrigation water and their effectiveness. The separation of interfering components via adsorption is effective and promising for future application as the expected irrigation demands increase. Full article

On-farm composting of agro-livestock wastes can be considered the most appropriate method for their recycling. Pile turning (PW) is one of the most widely used aeration systems for composting. However, this system has long composting periods and is inefficient at supplying oxygen and controlling the temperature. To minimize these drawbacks, the combination of turnings with forced aeration (PR) is an option; in this work, this combination was compared to PW as an aeration system for the co-composting of vegetable waste with different manures. In this comparative study, the evolution of the process, the compost quality and the economic and environmental impacts of the process were evaluated. The PR system was more appropriate for obtaining sanitized composts (the temperature was ≥55 °C for at least three consecutive days) with an adequate degree of maturity. Furthermore, this system reduced the organic matter and nutrient losses, yielding composts with higher agronomic value and a higher total combined value of the nutrients than those obtained using the PW system. However, the energy consumption and associated CO₂ emissions were lower for the PW system, since this aeration system was based only on turnings without the use of forced aeration, as in the case of the PR system. Agricultural valorization of composts will offset this energy consumption and its impact, since it will contribute to reducing the use of synthetic fertilizers. However, more studies are required on the PR composting system and other agro-livestock wastes for the creation of centralized on-farm composting sites, where all steps of the composting chain are optimized. Full article

Climate change and nutrient pollution are echoed by worldwide increasing trends in the frequency, duration, and toxicity of cyanobacterial (blue-green algal) blooms. Therefore, searching for the best options to mitigate blooms is relevant and timely. Aquatic vascular plants offer a promising solution through biological control. In this study, we use reservoirs regularly affected by intensive blooms (the Kyiv and Kaniv Reservoirs of the Dnipro River, Ukraine) to investigate whether macrophytes may inhibit or reduce the massive development of cyanobacteria. Special attention was paid to plants with floating leaves and free-floating plants since data on their effects on cyanobacteria are controversial. On the basis of field and satellite observations, the spatial distribution of cyanobacterial blooms and aquatic macrophyte patches was assessed. Multispectral images captured by satellites Sentinel-2a (S2A) and Sentinel-2b (S2B) were used. In addition, based on data from field observations, a comparative analysis of phytoplankton and physical and chemical parameters between areas of the reservoirs overgrown and not overgrown by macrophytes was carried out. The obtained results indicate that in macrophyte patches phytoplankton structure differed from that observed in open waters. However, in areas of reservoirs dominated by floating-leaf plants or free-floating plants, a significant decrease in phytoplanktic or cyanobacterial biomass was not observed. This is most likely due to the fact that these macrophytes did not reduce the concentration of biogenic substances to a level that would limit cyanobacterial growth. On the contrary, intensive overgrowth of floating-leaf plants (in particular, Trapa natans) along the river sections of the reservoirs, as well as other factors, contributed to nitrogen and phosphorus enrichment. Therefore, in the face of relevant nutrient supply, these ecological groups of macrophytes (floating-leaf plants and free-floating plants) have not shown statistically significant effectiveness in controlling the process of cyanobacterial blooms in reservoir ecosystems. Full article