Search Results (196)

Plastic pollution and wastewater have become the leading environmental concerns due to their harmful effects on human health and pose a severe threat to the biosphere. Polyethylene terephthalate (PET) is one of the most widely used plastics worldwide, but it is resistant to natural degradation. Additionally, the complex pollutants in wastewater demand advanced remediation strategies. Although physicochemical methods are commonly used for PET degradation and wastewater treatment, bioremediation with microorganisms offers a greener and more eco-friendly alternative. This review focuses on the molecular mechanisms and engineering of synthetic microbial consortia (SMC) for the bioremediation of wastewater and PET plastics. It examines the rational design of SMCs, utilizing both bottom-up and top-down methods, and emphasizes the importance of quorum sensing and metabolite cross-feeding in maintaining the stability and functionality of the consortium. Furthermore, the review critically assesses how CRISPR-Cas9 enables precise genome editing for robust pathway engineering and stress resilience, while Machine Learning provides predictive models to optimize consortium composition and function, thereby advancing SMC capabilities for both applications. These developments highlight SMC as a promising, eco-friendly, and efficient biological platform to tackle wastewater challenges and plastic pollution simultaneously. Full article

Colorectal cancer (CRC) is the fourth most common and the third mostly deadly cancer globally. Even with alternative therapies, some patients do not respond to treatment. Identifying modulations in the tumor microenvironment (TME) of CRC is a significant challenge due to the complex and dynamic nature of the TME. The intestinal epithelium comprises different types of secretory lineage cells, including goblet, tuft, Paneth, and enteroendocrine cells (EECs). Yet the relevance of each subtype of secretory intestinal epithelial cell (IEC) within the TME is still debated. This study investigated the involvement of IECs in CRC development through an integrative bioinformatics analysis. We used publicly available datasets from the National Center for Biotechnology Information, the Cancer Genome Atlas Program, and the National Cancer Institute’s Proteomics Tumor Analysis Consortium, encompassing both human and mouse CRC samples. Our findings reveal a CRC microenvironment characterized by elevated expression levels of genes associated with WNT pathway activity. Remarkably, there was increased expression of Paneth cell-associated markers and transcription factors, such as WISP1, LYZ, SOX9, and DEFA1. Conversely, EEC-specific gene markers, such as GCG (encoding glucagon-like peptide-1) and CHGA exhibited significant downregulation in CRC tissue compared with healthy tissue, partially due to Paneth cell activity. Gene ontology analysis showed species-conserved downregulation in hormone/peptide secretion-related pathways in both mouse and human CRC. Of note, lower levels of GCG and CHGA correlated with reduced overall survival and demonstrated a correlation with the cell cycle, apoptosis, and proliferation. These results suggest that the disruption of enteroendocrine cell signaling is a hallmark of CRC development and may hold prognostic and therapeutic value in treating CRC patients. Full article

In the booming digital economy, data circulation—particularly for massive multimodal data generated by IoT sensor networks—faces critical challenges: ambiguous ownership and broken cross-domain traceability. Traditional property rights theory, ill-suited to data’s non-rivalrous nature, leads to ownership fuzziness after multi-source fusion and traceability gaps in cross-organizational flows, hindering marketization. This study aims to establish native ownership confirmation capabilities in trusted IoT-driven data ecosystems. The approach involves a dual-factor system: the collaborative extraction of text (from sensor-generated inspection reports), numerical (from industrial sensor measurements), visual (from 3D scanning sensors), and spatio-temporal features (from GPS and IoT device logs) generates unique SHA-256 fingerprints (first factor), while RSA/ECDSA private key signatures (linked to sensor node identities) bind ownership (second factor). An intermediate state integrates these with metadata, supported by blockchain (consortium chain + IPFS) and cross-domain protocols optimized for IoT environments to ensure full-link traceability. This scheme, tailored to the characteristics of IoT sensor networks, breaks traditional ownership confirmation bottlenecks in multi-source fusion, demonstrating strong performance in ownership recognition, anti-tampering robustness, cross-domain traceability and encryption performance. It offers technical and theoretical support for standardized data components and the marketization of data elements within IoT ecosystems. Full article

Improving the quality of oil-contaminated soils remains a critical challenge, and bioaugmentation using allochthonous bacteria offers promising perspectives. This study proposes a framework for exogenous bioaugmentation using a bacterial consortium, composed of strains from diverse climates, immobilized in alginate beads and combined with calcium peroxide as an oxygen-releasing compound. Two conditions were tested: freshly prepared beads (BA) and lyophilized beads (LA). Their performance was compared to natural attenuation (NA) and to landfarming coupled with bioaugmentation using a free autochthonous consortium. Hydrocarbon degradation was assessed through total petroleum hydrocarbon (TPH) and alkane depletion (GC-MS), microbial community dynamics (amplicon sequencing), and abundance of the alkB gene (qPCR). In three months, the BA treatment achieved a 44% TPH reduction, outperforming LA (34%) and NA (10% less than BA). However, LA induced a marked increase in alkB gene copies and microbial biomass at the end of the experiment, suggesting greater long-term potential. Dominant genera varied across treatments: Rhodococcus in NA, Gordonia in BA, and Pseudomonas in LA. In parallel, the autochthonous consortium achieved up to 80% oil degradation. This study demonstrates the viability of lyophilized microbial consortia in scalable, ready-to-use formulations and provides an operational methodology for exogenous bioaugmentation as a tool for the remediation of hydrocarbon-contaminated soils. Full article

Aerobic soils serve as significant sinks for atmospheric methane, with their effectiveness influenced by the diversity and activity of soil methanotrophs. Land-use changes, particularly the conversion of natural ecosystems to agriculture, can substantially alter these microbial communities. A promising strategy to restore methane oxidation capacity is the introduction of active, ambient methane-oxidizing bacteria. The stable methane-oxidizing microbial consortium T1, dominated by Methylocystis (74%), was isolated from the soil of the unique Chernevaya Taiga forest ecosystem. The effects of inoculating this consortium were evaluated in a four week laboratory incubation experiment, using microcosms of soddy-podzolic agro-soil. Methane oxidation potential was assessed to measure methanotroph activity; methanotrophs were quantified using qPCR targeting pmoA genes; and the diversity of soil microbial communities was examined through 16S rRNA gene profiling. Inoculated soils exhibited significantly higher methane oxidation potentials compared to non-inoculated soils. Furthermore, pmoA gene copy numbers in the inoculated soils were significantly elevated (10⁶ copies pmoA g⁻¹), indicating stable persisted methanotrophic populations throughout the incubation period. These findings suggest that enriched methanotrophic consortium inoculation into agro-soils may be a promising strategy for restoring methane-oxidizing activity. Full article

The symbiotic microbiome constitutes a consortium that has been persistently domesticated by a specific algal species, fostering a close and enduring association with the host. The majority of microbial taxa remain uncharacterized. These unknown microbes, often referred to as “microbial dark matter (MDM)”, have important ecological contributions. Given the challenges in discerning symbiotic microbes in natural environments, herein, ecological characteristics of MDM and known taxa within symbiotic communities were investigated in a simulated bloom process using Alexandrium pacificum without antibiotic treatment. Specifically, increased diversification was observed in MDM along the bloom process. Higher trophic interaction and less vulnerability of the molecular network were found in MDM taxa. The “bridge” role of MDM species was better than that of known taxa, as shown by higher betweenness centralization. Deterministic processes dominated in MDM taxa, which promote phylogenic diversity of such groups to some extent. The findings highlight that MDM taxa play an important role in sustaining community stability and functioning. This study broadens our understanding of the ecological contribution of MDM under disturbances from dinoflagellate blooms, providing essential theoretical insights and empirical data to inform the management of coastal toxic blooms. Full article

The wastewater treatment (WWT) industry is currently facing challenges imposed by the revised urban WWT directive, particularly in terms of nitrogen (N) and phosphorus (P) removal. This implies the need for mandatory tertiary treatment, for which microalgae cultivation shows great sustainability promise. This study investigated the impact of hydraulic retention time (HRT) on nutrient removal in open-air microalgae cultivation for tertiary WWT under winter conditions. Two pilot-scale semi-continuous raceway systems were operated with indigenous microalgae, natural sunlight, and no pH control. HRT values of 4, 5.5, and 7 days were tested, and N, P, and carbon (C) removal and recovery were measured. All conditions allowed nitrogen removal, complying with the revised urban WWT directive. Regarding P, only the 7-day HRT condition consistently complied with the directive’s lowest limit (<0.5 mg P·L⁻¹) in the treated water, while 5.5 and 4 days left up to 0.7 and 1.0 mg P·L⁻¹, respectively, in up to 25% of the samples. A stable microalgae consortium was established under variable light, pH, and dissolved oxygen conditions, albeit with variable biomass productivity. Elemental mass balances revealed that nutrients were mostly recovered in the produced biomass, particularly at high HRT, including effective CO₂ capture from the atmosphere. Full article

The bacterial phylum Verrucomicrobiota accommodates free-living and symbiotic microorganisms, which inhabit a wide range of environments and specialize in polysaccharide degradation. Due to difficulties in cultivation, much of the currently available knowledge about these bacteria originated from cultivation-independent studies. A phylogenetic clade defined by the free-living bacterium from oilsands tailings pond, Oleiharenicola alkalitolerans, and the symbiont of the tunicate Lissoclinum sp., Candidatus Didemniditutus mandelae, is a poorly studied verrucomicrobial group. This clade includes two dozen methagenome-assembled genomes (MAGs) retrieved from aquatic and soil habitats all over the world. A new member of this clade, strain Vm1, was isolated from a methane-fed laboratory bioreactor with a Methylococcus-dominated methane-oxidizing consortium and characterized in this study. Strain Vm1 was represented by ultra-small, motile cocci with a mean diameter of 0.4 µm that grew in oxic and micro-oxic conditions at temperatures between 20 and 42 °C. Stable development of strain Vm1 in a co-culture with Methylococcus was due to the ability to utilize organic acids excreted by the methanotroph and its exopolysaccharides. The finished genome of strain Vm1 was 4.8 Mb in size and contained about 4200 predicted protein-coding sequences, including a wide repertoire of CAZyme-encoding genes. Among these CAZymes, two proteins presumably responsible for xylan and arabinan degradation, were encoded in several MAGs of Vm1-related free-living verrucomicrobia, thus offering an insight into the reasons behind wide distribution of these bacteria in the environment. Apparently, many representatives of the Oleiharenicola–Candidatus Didemniditutus clade may occur in nature in trophic associations with methanotrophic bacteria, thus participating in the cycling of methane-derived carbon. Full article

There is an urgent need for new approaches and strategies for the introduction of antioxidant drugs in medicine. Despite hundreds of clinical trials with potential antioxidants, no antioxidant drugs have so far been developed for clinical use; this is mainly as a result of commercial reasons, but also due to insufficient data for regulatory authority approval. Antioxidant activity is a physiological process essential for healthy living. However, increased production of toxic free radicals and reactive oxygen species is observed in many clinical conditions, which are associated with serious and sometimes irreversible damage. Antioxidant drug strategies may involve short- to long-term therapeutic applications for the purpose of prevention, treatment, or post-treatment effects of a disease. These strategies are different for each disease and may include the design of protocols for the inhibition of oxidative damage through iron chelation, enhancing antioxidant defences by increasing the production of endogenous antioxidants, and activating antioxidant mechanisms, as well as the administration of synthetic and natural antioxidants. Both the improvement of antioxidant biomarkers and clinical improvement or disease remission are required to suggest effective therapeutic intervention. More concerted efforts, including new academic strategies, are required for the development of antioxidant drugs in clinical practice. Such efforts should be similar to the fulfilment of orphan or emergency drug regulatory requirements, which, in most cases, involve the treatment or clinical improvement of rare or severe diseases such as neurodegenerative diseases and cancer. Promising results of antioxidant therapeutic interventions include mainly the repurposing of the iron chelating/antioxidants drugs deferiprone (L1) and deferoxamine, and also the iron-binding drug N-acetylcysteine (NAC). In some clinical trials, the lack of pharmacodynamic and ferrikinetic data, wrong posology, and insufficient monitoring have resulted in inconclusive findings. Future strategies involving appropriate protocols and drug combinations, such as L1 and NAC, appear to improve the prospect of developing antioxidant drug therapies in different diseases, including those associated with ferroptosis. New strategies may also involve the use of pro-drugs such as aspirin, which is partly biotransformed into iron chelating/antioxidant metabolites with chemopreventive properties in cancer, and also in other therapeutic interventions. A consortium of expert academics on regulatory drug affairs and clinical trials could increase the prospects for antioxidant drug development in medicine. Full article

Kombucha, a fermented tea beverage, is gaining popularity due to its rich content of bioactive compounds and associated health benefits. Kombucha fermentation involves a complex microbial consortium, including acetic acid bacteria, lactic acid bacteria, and yeasts, that works synergistically to enhance its nutritional and functional properties. Key compounds produced during fermentation provide antioxidant, anti-inflammatory, and antimicrobial benefits. Despite its well-documented health-promoting properties, limited research exists on how human digestion influences the stability and functionality of kombucha bioactive components. This study investigated how digestion impacts kombucha made from green and black teas, focusing on free amino acid content, antioxidant activity, antimicrobial potential, and microbiota viability. Results showed that digestion significantly increased free amino acids, as fermentation released peptides suitable for gastrointestinal digestion. However, L-theanine, a beneficial tea compound, was no longer detectable after fermentation and digestion, suggesting limited bioaccessibility. Digested kombucha exhibited higher antioxidant activity and stronger antimicrobial effects compared to undigested tea. Moreover, culture-dependent and PMA-based sequencing confirmed the survival of viable microbial strains through simulated gastrointestinal conditions, suggesting the potential of kombucha as a source of live, functional microbes. These findings support the role of kombucha as a natural functional beverage whose health benefits not only persist but may be enhanced after digestion. Full article

Carbon isotope fractionation is an efficient tool used for the discrimination and differentiation of sinks and emission sources. Carbon dioxide (CO₂) and methane (CH₄) are among the key drivers of climate change, and a detailed evaluation of variations in the ¹³C/¹²C ratio in either compound provides vital information for the field of atmospheric sciences. The Italian atmospheric ICOS (Integrated Carbon Observation System) consortium is currently implementing δ¹³C-CO₂ and δ¹³C-CH₄ measurements, with four observation sites now equipped with Picarro G2201-i CRDS (Cavity Ring-Down Spectrometry) analyzers. In this work, results from the first two months of measurements performed at the Potenza station in southern Italy between 20 February and 20 April 2025 are presented and constitute the first evaluation of continuous atmospheric δ¹³C-CO₂ and δ¹³C-CH₄ measurements from an Italian station. These results provide a first insight on how these measurements can improve the current understanding of CO₂ and CH₄ variability in the Italian peninsula and the central Mediterranean sector. Although preliminary in nature, the findings of these measurements indicate that fossil fuel burning is responsible for the observed peaks in CO₂ concentrations. CH₄ has a generally stable pattern; however, abrupt peaks in its isotopic delta, observed during March, may constitute the first direct evidence in Italy of Saharan dust intrusion affecting carbon isotope fractionation in the atmosphere. This study also introduces an analysis of the weekly behavior in isotopic deltas. Full article

The rubber industry is facing major socio-economic and environmental constraints. Rubber-based agroforestry systems represent a more sustainable solution through the diversification of income and the provision of greater ecosystem services than monoculture plantations. Participative approaches are known for their ability to co-construct solutions with stakeholders and to promote a positive impact on smallholders. This study therefore implemented a participatory research process with stakeholders in the natural rubber sector for the purpose of improving inclusion, relevance and impact. Facilitation training sessions were first organised with academic actors to prepare participatory workshops. A working group of stakeholder representatives was set up and participated in these workshops to share a common representation of the value chain and to identify problems and solutions for the sector in Indonesia. By fostering collective intelligence and systems thinking, the process is aimed at enabling the development of adaptive technical solutions and building capacity across the sector for future government replanting programmes. The resulting adaptive technical packages were then detailed and objectified by the academic consortium and are part of a participatory plant breeding approach adapted to the natural rubber industry. On-station and on-farm experimental plans have been set up to facilitate the drafting of projects for setting up field trials based on these outcomes. Research played a dual role as both knowledge provider and facilitator, guiding a co-learning process rooted in social inclusion, equity and ecological resilience. The initiative highlighted the potential of rubber cultivation to contribute to climate change mitigation and food sovereignty, provided that it can adapt through sustainable practices like agroforestry. Continued political and financial support is essential to sustain and scale these innovations. Full article

Background/Objectives: Epilepsy and sleep disturbances frequently co-occur, yet the causal nature of this relationship remains uncertain, particularly in relation to epilepsy subtypes and status epilepticus. We investigated potential bidirectional causal associations between sleep-related traits and epilepsy, including subtypes and status epilepticus, using Mendelian randomization (MR). Methods: We conducted two-sample MR using genome-wide association study (GWAS) summary statistics from European ancestry cohorts. Epilepsy, its subtypes, and status epilepticus were analyzed using data from the International League Against Epilepsy Consortium on Complex Epilepsies (ILAE) and the FinnGen study. Nine self-reported sleep-related traits were derived from the UK Biobank-based GWAS. Causal estimates were primarily obtained using inverse variance weighted models with additional MR analysis methods. Pleiotropy and heterogeneity were assessed to enhance the robustness of the finding. Results: Several subtype-specific associations were identified, with direction and statistical significance varying across cohorts and subtypes. After correction for multiple testing and filtering for tests with ≥10 instrumental variables to ensure robust and reliable MR estimates, several consistent and potentially mutually reinforcing associations emerged. In the ILAE cohort, focal epilepsy with hippocampal sclerosis was associated with an increased risk of insomnia, and juvenile myoclonic epilepsy with reduced sleep duration. In the FinnGen cohort, overall epilepsy was associated with increased risk of both insomnia and daytime sleepiness. In reverse MR, daytime sleepiness and napping were associated with increased risk of epilepsy, while daytime napping and frequent insomnia symptoms were linked to elevated risk of status epilepticus. Conclusions: Our findings reveal subtype-specific and bidirectional causal links between epilepsy and sleep-related traits. These results highlight the biological interplay between epileptic networks and sleep regulation and underscore the need for further clinical and mechanistic studies. Full article

The traditional fermentation of table olives is a complex and dynamic, process, carried out by a consortium of microorganisms that interact with each other and contribute to the uniqueness and attractiveness of the final product. Fermentation is conducted by yeasts and lactic acid bacteria (LAB) that coexist in olive fruits. The succession of one microbial population to the detriment of others depends on internal and external factors that affect the process, e.g., the maturation degree of fruits, cultivar, endophytic, or epiphytic state of microorganisms, pH, water activity, temperature, and salt concentration. Thus, studying microbiota evolution and their identification in natural table olive fermentations is paramount. This review aims to provide an overview of the knowledge on the natural fermentation of table olives, namely regarding microbial dynamics, as to report the main species involved in the fermentation process, highlight the influence of the olive oil ecosystem on the origin of the microbiota and consequently on the obtaining of the final product. The results report a total of 97 yeast species and 45 LAB species described in olives and brine over the last few decades. Full article

Electric vehicles have garnered substantial attention as an environmentally sustainable transportation alternative amid escalating global concerns regarding ecological preservation and energy resource management. While the proliferation of electric vehicles necessitates the development of efficient and secure charging infrastructure, the inherent communication-intensive nature of the charging processes has raised concerns regarding potential privacy vulnerabilities. Our paper introduces a privacy protection scheme specifically designed for electric vehicle charging reservations to address this issue. The primary goal of this scheme is to protect user privacy while maintaining operational efficiency and economic viability for charging providers. Our proposed solution ensures a secure and private environment for charging reservation transactions and subsequent deviation settlements by incorporating advanced technologies, including zero-knowledge proof, a consortium blockchain, and homomorphic encryption. The scheme encrypts charging reservation information and securely transmits it via a consortium blockchain, effectively shielding the sensitive data of all participating parties. Notably, the experimental findings establish the robustness of our scheme in terms of its security and privacy protection, aligning with the stringent demands of electric vehicle charging operations. Full article