Search Results (7)

The field of nanoparticle-based biotechnology has undergone substantial advancement, characterized by progress in targeted drug delivery systems, the development of innovative diagnostic and imaging platforms, the expanded adoption of environmentally sustainable (“green”) synthesis approaches, and an increasing emphasis on the integration of emerging technologies such as artificial intelligence and nanorobotics. Conventional nanoparticle synthesis often involves toxic reducing agents; however, recent advances promote eco-friendly green synthesis methods utilizing biological systems such as bacteria, fungi, algae, yeast, plants, and actinomycetes. These biological approaches are safe, sustainable, cost-effective, and capable of producing highly stable Nanoparticles (NPs). The interaction of nanomaterials with biological systems is crucial for developing intracellular and subcellular drug delivery technologies with minimal toxicity, governed by nano–bio interface mechanisms such as cellular translocation, surface wrapping, embedding, and internal attachment. Key factors influencing NP behavior include morphology, size, surface area, surface charge, and ligand chemistry. Magnetic nanoparticles, particularly iron-based forms, exhibit unique superparamagnetic properties that are strongly influenced by particle size, as explained by the Néel relaxation mechanism, in which thermal energy induces flipping of magnetic moments. Nanoparticles demonstrate diverse modes of action, including antimicrobial activity, reactive oxygen species (ROS)-induced cytotoxicity, genotoxicity, and plant growth promotion. NP performance and biological effects are strongly dependent on their size, shape, dosage, and concentration. This critical review article aims to elucidate evolution, classification, preparation methods, and multifaceted applications of nanoparticles. Full article

Background: Chemodynamic therapy (CDT) and photothermal therapy (PTT) based on nanomaterials have garnered widespread attention in cancer treatment. However, most single-modal nanotherapeutics suffer from limited therapeutic efficacy. Methods: Herein, a magnetic mesoporous composite nanoparticle, Fe₃O₄@MSN@PDA-HA-FA, is successfully fabricated, with Fe₃O₄ nanoparticles as the magnetic core; mesoporous silica nanoparticles (MSNs) as the mesoporous shell; and dopamine hydrochloride (DA·HCl), hyaluronic acid (HA), and folic acid (FA) as the functional ligands. Results: Notably, this composite serves as both an efficient photothermal converter and a chemodynamic promoter, enhancing hydroxyl radical (·OH) generation and improving PTT efficacy. Under near-infrared (NIR) light irradiation, Fe₃O₄@MSN@PDA-HA-FA exhibits high photothermal conversion and heat transfer efficiencies. The Fe²⁺ ions in Fe₃O₄ enable a Fenton reaction-mediated conversion of endogenous hydrogen peroxide (H₂O₂) into ·OH for CDT. Additionally, the MSNs provide a substantial drug-loading capacity, while the HA and FA provide additional surface functionalities that can modulate the nano-bio interactions and improve colloidal stability. Conclusions: In vitro experiments validate the synergistic therapeutic efficacy of PTT, CDT, and chemotherapy. This study demonstrates that Fe₃O₄@MSN@PDA-HA-FA exhibits antitumor efficacy, laying a promising foundation for its potential clinical translation in cancer treatment. Full article

African swine fever (ASF) is one of the most lethal infectious diseases affecting domestic pigs and wild boars of all ages. Over a span of 100 years, ASF has continued to spread over continents and adversely affects the global pig industry. To date, no vaccine or treatment has been approved. The complex genome structure and diverse variants facilitate the immune evasion of the ASF virus (ASFV). Recently, advanced technologies have been used to design various potential vaccine candidates and effective diagnostic tools. This review updates vaccine platforms that are currently being used worldwide, with a focus on genetically modified live attenuated vaccines, including an understanding of their potential efficacy and limitations of safety and stability. Furthermore, advanced ASFV detection technologies are presented that discuss and incorporate the challenges that remain to be addressed for conventional detection methods. We also highlight a nano-bio-based system that enhances sensitivity and specificity. A combination of prophylactic vaccines and point-of-care diagnostics can help effectively control the spread of ASFV. Full article

In this paper, glutathione (GSH)-coated Zn-doped CdTe quantum dots (QDs) with different particle sizes were synthesized using the “reflow method”, and the interaction mechanism between the two QDs and lactoferrin (LF) was investigated systemically with different spectroscopic methods. The steady-state fluorescence spectra showed that the LF formed a tight complex with the two QDs through static bursting and that the electrostatic force was the main driving force between the two LF–QDs systems. The complex generation process was found to be spontaneous (ΔG < 0) and accompanied by exothermic and increasing degrees of freedom (ΔH < 0, ΔS > 0) by using the temperature-dependent fluorescence spectroscopy. The critical transfer distance (R₀) and donor–acceptor distance (r) of the two LF–QDs systems were obtained based on the fluorescence resonance energy transfer theory. In addition, it was observed that the QDs changed the secondary and tertiary structures of LF, leading to an increase in the hydrophobicity of LF. Further, the nano-effect of orange QDs on LF is much larger than that of green QDs. The above results provide a basis for metal-doped QDs with LF in safe nano-bio applications. Full article

Dispersing particles in a liquid phase is significant for producing various functional nano/bio applications. The wet-jet milling method has been gaining attention as an attractive dispersing method in the preparation of soft material suspensions. This is because the main driving force of dispersion by the wet-jet milling method is the shear force, which is weaker than that it is in the ultrasonication dispersing method. In the wet-jet milling method, the pressure of the narrow channel which the liquid is passes through and the number of passes are used as the control parameters for dispersing the particles. However, the values of the pressure depend on the size (diameter and length) of the narrow channel, thus, it is not a commonly used dispersing parameter in dispersing by wet-jet milling to set the dispersing condition by various wet-jet milling instruments. In addition, wet-jet milling users must optimize the dispersing conditions such as the pressure and number of passes in the narrow channel, therefore, a simple prediction/optimization method of the dispersing size by the wet-jet milling method is desired. In this study, we established a novel colloidal dispersing concept, the dispersing energy input based on a calorimetric idea, for particle suspension preparation using the wet-jet milling method. The dispersing energy input by wet-jet milling was quantitatively calculated under various conditions during the dispersing by wet-jet milling, and then, the dispersing size of the particles was easily predicted/optimized. We demonstrated the usability of the concept by preparing aqueous suspensions of calcium carbonate (CaCO₃) particles with various surfactants using the wet-jet milling method. Based on the established concept, in a case study on dispersing CaCO₃, we found that changes in the micelle sizes of the surfactants played a role in wet-jet milling. The novel idea of the representation of energy input makes it possible to estimate the appropriate condition of the dispersing process by wet-jet milling to control the size of particles. Full article

Since the beginning of the 2000s, globalization has accelerated because of the development of transportation systems that allow for human and material exchanges throughout the world. However, this globalization has brought with it the rise of various pathogenic viral agents, such as Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), Zika virus, and Dengue virus. In particular, avian influenza virus (AIV) is highly infectious and causes economic, health, ethnical, and social problems to human beings, which has necessitated the development of an ultrasensitive and selective rapid-detection system of AIV. To prevent the damage associated with the spread of AIV, early detection and adequate treatment of AIV is key. There are traditional techniques that have been used to detect AIV in chickens, ducks, humans, and other living organisms. However, the development of a technique that allows for the more rapid diagnosis of AIV is still necessary. To achieve this goal, the present article reviews the use of an AIV biosensor employing nanobio hybrid materials to enhance the sensitivity and selectivity of the technique while also reducing the detection time and high-throughput process time. This review mainly focused on four techniques: the electrochemical detection system, electrical detection method, optical detection methods based on localized surface plasmon resonance, and fluorescence. Full article

Waterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study proposes an eco-friendly, safranin-tinted Candida rugosa lipase (triacylglycerol ester hydrolysis EC 3.1.1.3) with functionalized carbon nanotubes (CRL-MWCNTS/GA/SAF) as an alternative reagent to the small particle reagent. The CRL-MWCNTS/GA/SAF reagent was compared with the small particle reagent to visualize groomed, full fingerprints deposited on stainless steel knives which were immersed in a natural outdoor pond for 30 days. The quality of visualized fingerprints using the new reagent was similar (modified-Centre for Applied Science and Technology grade: 4; p > 0.05) to small particle reagent, even after 15 days of immersion. Despite the slight decrease in quality of visualized fingerprints using the CRL-MWCNTS/GA/SAF on the last three immersion periods, the fingerprints remained forensically identifiable (modified-Centre for Applied Science and Technology grade: 3). The possible chemical interactions that enabled successful visualization is also discussed. Thus, this novel reagent may provide a relatively greener alternative for the visualization of latent fingerprints on immersed non-porous objects. Full article