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Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond

1
Department of Chemistry, Faculty of Science, Universiti Teknologi Malaysia, Skudai 81310, Malaysia
2
Criminal Investigation Department, Criminal Intelligence (D4), Royal Malaysia Police, Kuala Lumpur 50560, Malaysia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(6), 1576; https://doi.org/10.3390/ijms19061576
Received: 14 May 2018 / Revised: 14 May 2018 / Accepted: 21 May 2018 / Published: 25 May 2018
(This article belongs to the Section Biochemistry)
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Abstract

Waterways are popular locations for the disposition of criminal evidence because the recovery of latent fingerprints from such evidence is difficult. Currently, small particle reagent is a method often used to visualize latent fingerprints containing carcinogenic and hazardous compounds. This study proposes an eco-friendly, safranin-tinted Candida rugosa lipase (triacylglycerol ester hydrolysis EC 3.1.1.3) with functionalized carbon nanotubes (CRL-MWCNTS/GA/SAF) as an alternative reagent to the small particle reagent. The CRL-MWCNTS/GA/SAF reagent was compared with the small particle reagent to visualize groomed, full fingerprints deposited on stainless steel knives which were immersed in a natural outdoor pond for 30 days. The quality of visualized fingerprints using the new reagent was similar (modified-Centre for Applied Science and Technology grade: 4; p > 0.05) to small particle reagent, even after 15 days of immersion. Despite the slight decrease in quality of visualized fingerprints using the CRL-MWCNTS/GA/SAF on the last three immersion periods, the fingerprints remained forensically identifiable (modified-Centre for Applied Science and Technology grade: 3). The possible chemical interactions that enabled successful visualization is also discussed. Thus, this novel reagent may provide a relatively greener alternative for the visualization of latent fingerprints on immersed non-porous objects. View Full-Text
Keywords: forensic science; latent fingerprints; non-porous objects; immersion; natural outdoor pond; Candida rugosa lipase nanoconjugates; nanobio-based method forensic science; latent fingerprints; non-porous objects; immersion; natural outdoor pond; Candida rugosa lipase nanoconjugates; nanobio-based method
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Azman, A.R.; Mahat, N.A.; Abdul Wahab, R.; Abdul Razak, F.I.; Hamzah, H.H. Novel Safranin-Tinted Candida rugosa Lipase Nanoconjugates Reagent for Visualizing Latent Fingerprints on Stainless Steel Knives Immersed in a Natural Outdoor Pond. Int. J. Mol. Sci. 2018, 19, 1576.

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