Search Results (59)

Microbially induced carbonate precipitation (MICP) offers an eco-friendly approach to stabilize porous materials. This study evaluates its feasibility for protecting agricultural drainage ditch slopes through laboratory tests. Liquid experiments assessed calcium carbonate (CaCO₃) precipitation rates under varying bacteria–cementation solution ratios (BCR), cementation solution concentrations (1–2 mol/L), and urease inhibitor (NBPT) contents (0–0.3%). Soil experiments further analyzed the effects of solidified layer thickness (4 cm vs. 8 cm) and curing cycles on soil stabilization. The results showed that CaCO₃ precipitation peaked at a BCR of 4:5 and declined when NBPT exceeded 0.1%. Optimal parameters (0.1% NBPT, 1 mol/L cementation solution, BCR 4:5) were applied to soil tests, revealing that multi-cycle treatments enhanced soil water retention and CaCO₃ content (up to 7.6%) and reduced disintegration rates (by 70%) and permeability (by 83%). A 4 cm solidified layer achieved higher Ca²⁺ utilization, while an 8 cm layer matched or exceeded 4 cm performance with shorter curing. Calcite crystals dominated CaCO₃ formation. Crucially, reagent dosage should approximate four times the target layer’s requirement to ensure efficacy. These findings demonstrate that MICP, when optimized, effectively stabilizes ditch slopes using minimal reagents, providing a sustainable strategy for agricultural soil conservation. Full article

Erosion poses significant threats to infrastructures and ecosystems, exacerbated by climate change-driven sea-level rise and intensified wave actions. Microbially induced calcium carbonate precipitation (MICP) has emerged as a promising, sustainable, and eco-friendly solution for erosion mitigation. This review synthesizes recent advancements in optimizing biomineralization efficiency, multi-scale erosion control, and field-scale MICP implementations in marine dynamic conditions. Key findings include the following: (1) Kinetic analysis of Ca²⁺ conversion confirmed complete ion utilization within 24 h under optimized PA concentration (3%), resulting in a compressive strength of 2.76 MPa after five treatment cycles in ISO-standard sand. (2) Field validations in Ahoskie and Sanya demonstrated the efficacy of MICP in coastal erosion control through tailored delivery systems and environmental adaptations. Sanya’s studies highlighted seawater-compatible MICP solutions, achieving maximum 1743 kPa penetration resistance in the atmospheric zone and layered “M-shaped” CaCO₃ precipitation in tidal regions. (3) Experimental studies revealed that MICP treatments (2–4 cycles) reduced maximum scour depth by 84–100% under unidirectional currents (0.3 m/s) with the maximum surface CaCO₃ content reaching 3.8%. (4) Numerical simulations revealed MICP enhanced seabed stability by increasing vertical effective stress and reducing pore pressure. Comparative analysis demonstrates that while the destabilization depth of untreated seabed exhibits a linear correlation with wave height increments, MICP-treated seabed formations maintain exceptional stability through cohesion-enhancing properties, even when subjected to progressively intensified wave forces. This review supports the use of biomineralization as a sustainable alternative for shoreline protection, seabed stabilization, and offshore foundation integrity. Full article

Cohesive soil in the reservoir area is vulnerable to natural disasters because of its poor erosion resistance and low strength. Therefore, it needs to be reinforced. Microbially induced calcium carbonate precipitation (MICP) is a sustaibable soil reinforcement technique with low energy consumption and no pollution. Different combinations of Bacillus subtilis bacterial solution (BS) concentrations and cementing solution (CS) concentrations were set to perform MICP solidification treatment. The characterization of cohesive soil before MICP was carried out by means of Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), and Laser Particle Size Analyzer (LPSA). The results showed that the unreinforced soil showed an amorphous state with low strength and the particle size distribution was dominated by powder particles. However, with the addition of BS concentrations and CS concentrations, SEM results showed that spherical and rhombohedral minerals filled the pores of the cohesive soil, which increased the content of precipitations and enhanced the cementitious characteristics. When the concentrations of CS or BS were fixed, CaCO₃ content, deviatoric stress, shear strength, cohesive force, and internal friction angle all showed a trend of first increasing and then decreasing with the increase in CS or BS concentration. The optimal combination of CS and BS concentration was 1.5 mol/L and OD₆₀₀ = 1.8. Thermochemical analyses showed an improved thermal stability of the reinforcing cohesive soil, with the lowest mass loss (32%) and the highest pyrolysis temperature (812 °C) of the samples at the optimal combination of BS and CS concentration. This study is expected to improve the understanding of the MICP reinforcement process and contribute to the optimal design of future biologically mediated soil amendments, promoting bioremediation. Full article

The formation and spatial uniformity of calcium carbonate (CaCO₃) are critical for evaluating the effectiveness of microbial-induced calcium carbonate precipitation (MICP) in geotechnical applications. In recent years, the single-phase injection method has emerged as a promising alternative to traditional two-phase processes by addressing the issue of uneven CaCO₃ distribution. This study proposes a dual inhibition strategy that delays the mineralization reaction by synergistically lowering pH and temperature, thereby promoting uniform precipitation and enhanced compressive strength in cemented sand columns. A series of experiments, including bacterial growth, aqueous reaction, sand column reinforcement, and microstructural characterization, were conducted. Results show that the minimum pH required for flocculation increases from ~4.5 at 40 °C to ~6.0 at 10 °C. Under dual inhibition, the lag period effectively improved the spatial uniformity of CaCO₃ and enabled complete calcium utilization within 24 h. After four treatment cycles, the CaCO₃ content at 10 °C increased by 53%, and the unconfined compressive strength reached 2.5 MPa, a 50% improvement over the 40 °C condition. XRD analysis confirmed that calcite was the dominant phase (85–90%), accompanied by minor vaterite. These findings demonstrate the adaptability and efficiency of the dual inhibition method across temperature ranges, providing a cost-effective solution for broader engineering applications. Full article

Collapsible loess poses significant geotechnical risks due to its metastable structure and water sensitivity, while conventional stabilization methods often lack sustainability. This study investigates the synergistic effects of microbial-induced carbonate precipitation (MICP) and modified biochar (MBC) to enhance loess engineering properties. Controlled experiments evaluated hydraulic conductivity, shear strength, and stress-strress–strain behavior under varying MBC content (0–8%), cementation reagent concentration (0.5–1.5 mol/L), and confining pressures (50–400 kPa), and complemented by microstructural characterization via scanning electron microscope (SEM). Results demonstrate that MBC (4–6%) optimizes calcium carbonate distribution by providing nucleation sites, reducing hydraulic conductivity by 72% and increasing shear strength by 52% when compared with untreated loess. Elevated confining pressures (200–400 kPa) transformed brittle failure into ductile behavior through particle interlocking, with peak strength quadrupling under 400 kPa. SEM analysis revealed MBC stabilizes hierarchical pore networks: macropores sustain microbial activity, while mesopores are occluded by CaCO₃-MBC composites, sequestering ionic byproducts to mitigate efflorescence. The optimal combination (6% MBC, 1.0 mol/L reagent, 200 kPa confinement) achieved 85% of maximum strength gain at reduced reagent cost, balancing performance and sustainability. Full article

Microbially induced carbonate precipitation (MICP) is the precipitation of CaCO₃ crystals, induced by microbial metabolic activities such as ureolysis. Various applications of MICP have been proposed as innovative biocementation techniques. This study aimed to verify the feasibility of ureolysis-driven MICP applications in deep-subsurface environments (e.g., enhanced oil recovery and geological carbon sequestration). To this end, we screened sludge collected from a high-temperature anaerobic digester for facultatively anaerobic thermophilic bacteria possessing ureolytic activity. Then, we examined the ureolysis-driven MICP using a representative isolate, Bacillus haynesii strain SK1, under aerobic, anoxic, and strict anaerobic conditions at 30 °C, 40 °C, and 50 °C. All cultures showed ureolysis and the formation of insoluble precipitates. Fourier transform infrared spectroscopy analysis revealed precipitates comprising CaCO₃ at 30 °C, 40 °C, and 50 °C under aerobic conditions but only at 50 °C under anoxic and strict anaerobic conditions, suggesting efficient MICP at 50 °C. Interestingly, an X-ray diffraction analysis indicated that calcium carbonate crystals that were produced under aerobic conditions were in the form of calcite, while those that were produced under anoxic and strict anaerobic conditions at 50 °C were mostly in the form of vaterite. Thus, we demonstrated ureolysis-driven MICP under high-temperature and O₂-depletion conditions, suggesting the potential of MICP applications in deep-subsurface environments. Full article

The utilization of fossil fuels releases a large amount of carbon dioxide (CO₂) gas, leading to global temperature changes and climate warming. Carbon dioxide geological sequestration (CCS) is an effective solution, including the use of shallow seabed hydrate reservoirs as a geological sink. However, the sealing and strength of the caprock affect the sequestration effectiveness. Therefore, this study assessed the strength and sealing properties of a shallow seabed layer reinforced with Microbial-induced Carbonate Precipitation (MICP) technology through a combination of triaxial tests and X-ray CT. In addition, carbon dioxide sequestration experiments were conducted to investigate the factors influencing the ability of MICP technology to accelerate the mineralization and sequestration of carbon dioxide. The results demonstrate that MICP technology can enhance the sealing capacity of caprock by increasing its strength, reducing its porosity, and accelerating CO₂ mineralization. After 120 h of treatment, the CO₂ concentration in the air decreased from 887 ppm to 310 ppm, showing a significant mineralization effect. The bacteria used, Bacillus megaterium, can simultaneously secrete urease and carbonic anhydrase (CA). During the urease hydrolysis of urea, this not only increases the rate of calcium carbonate formation and improves the sealing performance but also accelerates the catalytic mineralization of CO₂ by carbonic anhydrase by creating an alkaline environment. Full article

Mineral carbonation is a prominent method for carbon sequestration. Atmospheric carbon dioxide (CO₂) is trapped as mineral carbonate precipitates, which are geochemically, geologically, and thermodynamically stable. Carbonate rocks can originate from biogenic or abiogenic origin, whereby the former refers to the breakdown of biofragments and the latter precipitation out of water. Carbonates can also be formed through biologically controlled mechanisms (BCMs), biologically mediated mechanisms (BMMs), and biologically induced mechanisms (BIMs). Microbial carbonate precipitation (MCP) is a BMM occurring through the interaction of organics (extracellular polymeric substances (EPS), cell wall, etc.) and soluble cations facilitating indirect precipitation of carbonate minerals. Microbially induced carbonate precipitation (MICP) is a BIM occurring via different metabolic pathways. Enzyme-driven pathways (carbonic anhydrase (CA) and/or urease), specifically, are promising for the high conversion to calcium carbonate (CaCO₃) precipitation, trapping large quantities of gaseous CO₂. These carbonate precipitates can trap CO₂ via mineral trapping, solubility trapping, and formation trapping and aid in CO₂ leakage reduction in geologic carbon sequestration. Additional experimental research is required to assess the feasibility of MICP for carbon sequestration at large scale for long-term stability of precipitates. Laboratory-scale evaluation can provide preliminary data on preferable metabolic pathways for different materials and their capacity for carbonate precipitation via atmospheric CO₂ versus injected CO₂. Full article

Microbial-induced calcium carbonate precipitation is an efficient and environmentally friendly soil stabilization technology. To explore the mineralization performance of carbonate precipitation, this study selects three factors, including the type of cementing solution (TCS), the cementing solution concentration (CSC), and the ratio of bacteria to cementing solution (B/C ratio), to investigate their effects on microbial mineralization. This study reveals the influence of each factor on the amount and rate of carbonate precipitation and analyzes the changes in the characteristics of carbonate precipitation crystals, such as the crystal diameter. The experimental results show that (1) the mineralization effect of magnesium ions and calcium ions results in higher precipitation amounts and rates than copper ions, with less environmental pollution. The concentration of the grout solution is positively correlated with the precipitation amount and negatively correlated with the precipitation rate, while the B/C ratio shows the opposite trend. (2) The crystal diameter of CaCO₃ between crystals reduces as the B/C ratio decreases and the CSC increases. (3) The characteristics of MgCO₃ crystals are mainly affected by the CSC. Both excessively high and low concentrations lead to an increase in crystal diameter. (4) The characteristics of CuCO₃ crystals are relatively stable, with smaller crystal particles maintained at around 1 μm. This study can provide a reference for the reinforcement of different types of soils, offering optimal reinforcement solutions based on the required crystal size, carbonate generation amount, and generation rate. It reduces resource waste and unnecessary chemical use, providing a theoretical foundation for sustainable soil remediation and ecological construction. Full article

This study investigates the potential of microbial-induced calcium carbonate precipitation (MICP) for soil stabilization and heavy metal immobilization, utilizing landfill leachate-derived ureolytic consortium. Experimental conditions identified yeast extract-based media as most effective for bacterial growth, urease activity, and calcite formation compared to nutrient broth and brown sugar media. Optimal MICP conditions, at pH 8–9 and 30 °C, supported the most efficient biomineralization. The process facilitated the removal of Cd²⁺ (99.10%) and Ni²⁺ (78.33%) while producing stable calcite crystals that enhanced soil strength. Thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)) confirmed the successful production of CaCO₃ and its role in improving soil stability. DSC analysis revealed endothermic and exothermic peaks, including a significant exothermic peak at 444 °C, corresponding to the thermal decomposition of CaCO₃ into CO₂ and CaO, confirming calcite formation. TGA results showed steady weight loss, consistent with the breakdown of CaCO₃, supporting the formation of stable carbonates. The MICP treatment significantly increased soil strength, with the highest surface strength observed at 440 psi, correlating with the highest CaCO₃ content (18.83%). These findings underscore the effectiveness of MICP in soil stabilization, pollutant removal, and improving geotechnical properties. Full article

Microbially induced calcium carbonate precipitation (MICP) is a technique used in geotechnical engineering to reinforce soil and rock. While it is commonly used for soil reinforcement, its application for rock reinforcement in saline–alkaline environments is limited. In order to improve the reinforcement effect of microbially induced calcium carbonate on rock joints in saline–alkaline environments, experiments were conducted to cultivate Sporosarcina pasteurii. The strengthening effects of MICP on rock joints were evaluated using the direct shear test. Samples of sandstone with rough surfaces were reinforced by MICP. The shear strength characteristics of rock joints reinforced by CaCO₃ were then assessed. The results showed that after being domesticated in a saline–alkaline environment, the bacterial concentration reached over 96% of that in a neutral environment. The domesticated Sporosarcina pasteurii performed well at temperatures between 10~30 °C in saline–alkaline conditions. In the saline–alkaline environment, the shear strength of rock joints and the production rate of CaCO₃ were higher, and the Sporosarcina pasteurii with domestication showed better joint repair performance. The peak shear strength of rock joints reinforced by MICP increased with curing time, with a quicker strength development in the early stage and a slower increase later on. The peak shear strength of cemented rock joints significantly surpassed that of uncemented rock joints. This research can provide valuable insights for the application of MICP technology in reinforcing rock joints in saline–alkaline environment. Full article

In order to prevent structural damage or high repair costs caused by concrete crack propagation, the use of microbial-induced CaCO₃ precipitation to repair concrete cracks has been a hot topic in recent years. However, due to environmental constraints such as oxygen concentration, the width and depth of repaired cracks are seriously insufficient, which affects the further development of microbial self-healing agents. In this paper, a ternary microbial self-healing agent composed of different proportions of Bacillus pasteurii, Saccharomyces cerevisiae, and Bacillus mucilaginosus was designed, and its crack repair ability was evaluated. When the mixing ratio was 7:1:2, the cell concentration was the highest, the precipitation amount of CaCO₃ was the highest, and the crystallinity of calcite crystal was the highest. Compared to the single microorganism, the mortar specimens with ternary microorganisms had the largest repair area (up to 100%) and the deepest repair depth (CaCO₃ presents at 9–12 mm from the crack surface). This is because when the concrete breaks, all three microorganisms are activated by water, O_2, and CO₂. Saccharomyces cerevisiae and Bacillus mucilaginosus accelerated the growth of Bacillus pasteurii and more mineralized products; CaCO₃ was rapidly formed and quickly filled on the crack surface. When CaCO₃ seals the surface of the crack, the internal Saccharomyces cerevisiae and Bacillus mucilaginosus continue to play a role. Bacillus mucilaginosus can accelerate the dissolution of CO₂ produced by the anaerobic fermentation of Saccharomyces cerevisiae and the hydrolysis of CO₃²⁻, thereby improving the repair of the crack depth direction. Full article

Microbial-induced calcium carbonate precipitation (MICP) can enhance the physical properties of recycled aggregates. Compared to traditional technologies, MICP offers environmental benefits and produces no pollution. However, its mineralization efficacy is significantly influenced by the process parameters. To investigate this, an MICP mineralization test was conducted by manipulating various process parameters throughout the mineralization process. The water absorption rate, apparent density, and calcium carbonate content of the mineralized recycled aggregates were assessed to discern the impact of these parameters on the mineralization outcome. Further analysis using techniques such as thermogravimetric analysis (TG), X-ray diffraction (XRD), nuclear magnetic resonance (NMR), and scanning electron microscopy (SEM) were employed to elucidate the mineralization mechanism of the recycled aggregates at a micro-level. The findings indicated that the MICP treatment induced bacteria to precipitate CaCO₃, forming calcite crystalline CaCO₃ within the pores and microcracks. This led to a denser interfacial transition zone and, consequently, improved the physical properties of the recycled aggregates. Optimal mineralization was achieved when the bacterial solution concentration was 1.4, the temperature and pH were 35 °C and 9, respectively, and the urea concentration, Ca⁺ concentration, and mineralization time were 0.5 mol/L, 0.5 mol/L, and 7 days, respectively. Under these conditions, the mineralized recycled aggregate exhibited a 16.07% reduction in water absorption, a 1.07% increase in apparent density, and a 2.28% change in mass. Full article

Granite residual soil is widely distributed in Southeastern China. Such soils exhibit mechanical characteristics such as loose, rich cracks and easy disintegration, resulting in severe soil erosion disasters under rainfall conditions. Microbial-induced carbonate precipitation (MICP) is a green alternative for soil stabilization. In this study, a new strategy for the disintegration control of granite residual soil using MICP technology is proposed. The effects of the bacterial solution concentration, the cementation solution concentration, and the treatment cycle are investigated through a disintegration test. The optimal treatment parameters for granite residual soil using MICP technology are determined by analyzing the disintegration processes and residual quality indicators of disintegration. The results show that the treated samples have three types of disintegration: complete disintegration, incomplete disintegration, and non-disintegration. The precipitated calcium carbonate (CaCO₃) bonds the soil particles and fills the pores. Taking into account the effectiveness and cost and a bacterial solution concentration OD600 = 0.75, five cycles of MICP treatment with a cementation solution concentration of 1.2 mol/L is optimal for the disintegration control of granite residual soil. The cementation-action effects of CaCO₃ are verified through scanning electron microscopy (SEM) tests with an energy-dispersive X-ray (EDX) spectroscope. These findings suggest that MICP is a promising candidate to control the disintegration of granite residual soil. Full article

To explore the effects of the introduction order of calcium sources and the bacteria-to-calcium ratio on the microbially induced calcium carbonate precipitation (MICP) product CaCO₃ and to achieve the regulation of CaCO₃ crystal morphology, the mineralisation products of MICP were compared after combining bacteria and calcium at ratios of 1/9, 2/9, 3/9, 4/9, 5/9, and 6/9. A bacterial solution was combined with a urea solution in two calcium addition modes: calcium-first and calcium-later modes. Finally, under the calcium-first addition method, the output of high-purity vaterite-type CaCO₃ was achieved at bacteria-to-calcium ratios of 2/9 and 3/9; under the calcium-later addition method, the output of calcite-type CaCO₃ could be stabilised, and the change in the bacteria-to-calcium ratio did not have much effect on its crystalline shape. Full article