Search Results (71)

Butidae is a family of teleost fishes with diverse morphological and ecological adaptations, including the marbled goby (Oxyeleotris marmorata), a large species of high economic value in Southeast and East Asia. The previous mitogenomic studies on cultured populations of O. marmorata from non-native habitats have provided limited insights into genetic divergence, structural variation, and evolutionary relationships. Hence, this study presented the complete mitochondrial genome of O. marmorata from its native habitat in Indonesia, providing structural characterization, assessment of genetic diversity, and matrilineal phylogenetic analysis. The circular mitogenome was 16,525 bp, comprising 37 genes and a non-coding control region (CR). The gene organization and strand distribution were conserved among Oxyeleotris species, with 28 genes on the heavy strand and nine on the light strand, and a pronounced A+T compositional bias. The comparative analyses of O. marmorata (from both native and cultured habitats) and Oxyeleotris lineolata mitogenomes revealed minor variations in intergenic spacers, gene overlaps, protein-coding gene (PCGs) lengths, and codon usage patterns. Conversely, the nonsynonymous and synonymous substitution ratios observed in species of the family Butidae and its closest related family (Eleotridae) indicate strong purifying selection in the present dataset. Notably, the ATG was the predominant start codon, whereas the COI gene utilized GTG, and amino acid composition analysis demonstrated high frequencies of arginine, leucine, and serine. Most transfer RNAs retained the canonical cloverleaf secondary structure except for trnS1, which lacked a functional dihydrouridine arm, whereas the CR contained four conserved sequence blocks with variable nucleotide motifs and no detectable tandem repeats. The haplotype analysis of native (Indonesia) and introduced populations (China) highlighted three haplotypes with high diversity (Hd = 1.0000) and substantial nucleotide variation (π = 0.6667). The genetic divergence across 13 PCGs was gene-specific, with COI and ND5 showing the highest variation, while ND4L and ATP8 were highly conserved. The phylogenetic analyses based on concatenated 13 PCGs using both Bayesian Inference and Maximum Likelihood methods revealed that Oxyeleotris forms a monophyletic clade and is closely related to Bostrychus sinensis. In addition, the broader phylogenetic framework inferred the matrilineal relationships within the family Butidae and its closest related family, Eleotridae. This study also recommends expanding analyses to include the mitogenomes of the remaining 17 Oxyeleotris species, together with comprehensive genomic data, to further elucidate their genetic architecture, evolutionary history, and ecological adaptability across diverse aquatic ecosystems. Full article

This study is part of a broader conservation and restoration project of the 17th-century statue “Santissimo Salvatore” attributed to the Bolognese sculptor Gabriele Brunelli (1615–1682). This sculpture was traditionally classified as a marble statue, i.e., primarily composed of calcium carbonate. However, the careful diagnostic analyses conducted during the present work of restoration revealed that, instead, the sculpture is made of gypsum alabaster, a material predominantly composed of calcium sulphate hydrate (CaSO₄·2H₂O). In the present research, a multi-analytical investigation was carried out using X-Ray Powder Diffraction (XRPD), Field Emission Environmental Scanning Electron Microscopy (FE-ESEM) with Energy-Dispersive X-ray Spectroscopy (EDS), and confocal Raman microspectrometry. Here, we report detailed and updated analytical data of the material constituting the “Santissimo Salvatore” statue by Gabriele Brunelli. These data were found extremely useful to plan and accomplish the restoration work in detail: (i) the suitable conservation project of the artwork, (ii) the reassessment of the knowledge on the artist’s sculptural production, and (iii) gaining more information about the material used in the 17th-century Bolognese sculptural context. Full article

Stone relics exposed to outdoor environments frequently experience surface deterioration, with red stains being a common and persistent issue. The stains often observed on marble and limestone surfaces arise from complex interactions involving chemical reaction, pollutant deposition, and microbiological process. Although microbial colonization has been associated with biodeterioration, the specific mechanisms remain poorly understood. This study focuses on the red stains found on the Danbi marble carvings at Beijing Confucian Temple and the Imperial College. Combining microbial cultivation, molecular identification (ITS sequencing), SEM-EDS (Scanning Electron Microscopy), Raman spectroscopy, and HPLC-MS (high-performance liquid chromatography with mass spectrometry), we identified the pigment-producing fungus Lizonia empirigonia as the dominant agent, with no evidence of inorganic contributors such as iron/lead oxides. Metabolite profiling revealed flavonoids and polyketides as key coloring material, while controlled infection experiments demonstrated the fungus’s reliance on exogenous organic matter rather than direct stone degradation. Our findings highlight microbial activity as a primary driver of red stains in marble relics and underscore the importance of organic contaminant control in conservation. Full article

Environmental stressors, such as freeze–thaw (F–T) cycling and acid rain, affect the durability of carbonate rocks used in engineering and cultural heritage structures. This study investigates the mechanical degradation and strain evolution of Carrara marble subjected to 10 F–T cycles and immersion in a simulated sulfuric acid solution (pH 5) for 3, 7, and 28 days. The mechanical strength of the samples was tested under uniaxial compression using a displacement-controlled loading rate, while full-field deformation and fracture evolution were analyzed with Digital Image Correlation (DIC). Results show that F–T cycling led to a substantial reduction in uniaxial compressive strength (UCS) and a very large decrease in tangent Young’s modulus. Acid exposure also caused progressive degradation, with both UCS and stiffness continuing to decline as exposure time increased, reaching their greatest reduction at the longest treatment duration. Additionally, DIC strain maps revealed a change in deformation response as a function of the treatment. The findings provide the integrated assessment of Carrara marble mechanical response under both F–T and acid weathering, linking bulk strength loss with changes in strain localization behavior, highlighting the vulnerability of marble to environmental stressors, and providing mechanical insights relevant to infrastructure resilience and heritage conservation. Full article

The conservation of heritage buildings requires non-invasive tools that can predict material performance while maintaining historical integrity and structural safety. This study introduces a multivariate statistical framework that integrates regression analysis, Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA) to classify seven traditional materials adobe, lime mortar, limestone, sandstone, marble, volcanic stone, and wood based on their mechanical, thermal, and moisture-related properties. This study aims to develop a validated multivariate framework for classifying traditional heritage materials based on their mechanical, thermal, and moisture-related properties to support sustainable restoration and retrofit design for classifying traditional materials based on their mechanical, thermal, and moisture-related properties to support sustainable restoration and retrofit design. Unlike prior research limited to single-material assessments, this study standardizes and analyzes data from fourteen peer-reviewed sources using regression models, Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA), Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA), complemented by pilot non-destructive validation tests on lime mortar, sandstone, limestone, and marble. The framework compiles and standardizes datasets from fourteen peer-reviewed sources into a unified predictive model. The framework was validated through pilot testing using non-invasive methods (density, ultrasonic pulse velocity, rebound hardness), which confirmed the statistical predictions of robustness versus moisture vulnerability. Advanced cluster solutions identified conservation-relevant subgroups, enabling engineers to distinguish between moisture-sensitive low-density materials and durable lithic stones, with direct implications for sustainable restoration and retrofit practices. The originality of this study lies in transforming fragmented datasets into a validated, decision-support tool that can be embedded into Historic Building Information Modeling (HBIM) platforms for predictive diagnostics, compatibility assessment, and energy-efficient retrofit planning in heritage structures. This study provides the first validated cross-material statistical framework linking traditional conservation materials with predictive digital-modeling tools. This framework further demonstrates that the application of regression, Principal Component Analysis (PCA), and Hierarchical Cluster Analysis (HCA) enables quantitative prediction of material performance through non-destructive parameters. The integration of these techniques provides interpretive value beyond descriptive classification, facilitating preventive diagnostics, compatibility assessments, and energy-oriented retrofit planning within HBIM systems. Full article

Monitoring humidity is essential for the protection and long-term preservation of historical monuments and cultural heritage structures, particularly those made of stone, marble, or iron. Excess moisture can accelerate material degradation and compromise structural integrity. This paper presents an alternative, low-cost method for enhancing the sensitivity of a raindrop sensor, aiming to detect micro-droplets such as early morning dew—an important factor in environmental monitoring around such sensitive sites. The proposed method involves covering the sensor’s surface with a fine nylon mesh, such as a stocking, which allows tiny water droplets to accumulate and spread more effectively across the sensor. This modification improves the electrical conductivity between the copper tracks when droplets are present, enabling the sensor to detect moisture levels that would otherwise go unnoticed. Experimental results demonstrate that the modified sensor performs significantly better than the original, unaltered version, offering greater sensitivity and consistency in its readings. The sensor responds more reliably to low volumes of moisture without requiring internal changes to its circuitry, making it both practical and cost-effective. The outcomes of this work are encouraging, suggesting that this approach is suitable for moisture detection in both research and real-world conservation scenarios. It provides a simple and scalable solution for integrating humidity monitoring into broader environmental sensing systems. Full article

This research investigates the feasibility of producing eco-friendly self-compacting concrete (SCC) by partially replacing both fine and coarse natural aggregates with waste marble sludge (WMS), a byproduct of the marble industry. The objective is to evaluate whether this substitution enhances or compromises the concrete’s performance while contributing to sustainability. A comprehensive experimental program was conducted to assess fresh and hardened properties of SCC with varying WMS content. Fresh-state tests—including slump flow, T₅₀ time, and V-funnel flow time—were used to evaluate workability, flowability, and viscosity. Hardened properties were measured through compressive, flexural, and Brazilian tensile strengths, along with water absorption after 28 days of curing. The mix with 10% replacement of both sand and coarse aggregate showed the most balanced performance, achieving a slump flow of 690 mm and a V-funnel time of 6 s, alongside enhanced mechanical properties—compressive strength 48.6 MPa, tensile strength 3.9 MPa, and flexural strength 4.5 MPa—and reduced water absorption (4.9%). A complementary cost model quantified direct material cost per cubic meter and a performance-normalized efficiency metric (compressive strength per cost). The cost decreased monotonically from 99.1 $/m³ for the base mix to $90.7 $/m³ at 20% + 20% WMS (−8.4% overall), while the strength-per-cost peaked at the 10% + 10% mix (0.51 MPa/USD; +12% vs. base). Results demonstrate that WMS can simultaneously improve rheology and mechanical performance and reduce material cost, offering a practical pathway for resource conservation and circular economy concrete production. Full article

The protection of masterpieces from natural hazards is one of the most important goals regarding heritage conservation. This work studies the seismic response of “Marzocco” by Donatello, which is the heraldic symbol of Florence, currently exposed at the National Museum of Bargello (Florence, Italy). The sculpture stands over a marble base and a magnificent marble pedestal by Benedetto da Maiano, which is part of the piece of art. The artwork is placed on the first floor of the museum, and it is subjected to seismic accelerations, eventually amplified by the seismic response of the building itself. The dynamic response of Marzocco is checked with reference to the overturning and sliding collapse mechanisms. Three overturning models have been considered, involving respectively the statue only, the statue and the base, and the statue with base and pedestal. The seismic input has been assumed through an ensemble of seven ground motions, which is spectrum-compatible with the Code’s one. Both the acceleration histories at the ground and at the floor have been considered. The seismic response has been found by performing a time-history analysis on a finite element model. The analysis showed a satisfactory seismic performance of the case study, pointing out the role of the single modeling assumptions. Full article

Natural carbonate stones such as limestones and marbles are widely used in heritage and contemporary architecture, yet their durability is increasingly threatened by wildfire-related thermal stress. Since water transport plays a key role in stone deterioration, understanding how high temperatures affect hydric behavior is critical for conservation. This study investigates thirteen Portuguese carbonate lithotypes (including marbles, limestones, a travertine, and a breccia) exposed to temperatures of 300 °C and 600 °C. Capillary absorption and open porosity were measured, alongside Leeb hardness (HL) and ultrasonic pulse velocity (UPV), to evaluate their predictive capacity for post-fire moisture behavior. Results show that thermal exposure increases porosity and capillary uptake while reducing mechanical cohesion. Strong correlations between UPV and hydric parameters across temperature ranges highlight its reliability as a non-invasive diagnostic tool. HL performed well in compact stones but was less consistent in porous or heterogeneous lithologies. The findings support the use of NDT tests, like UPV and HL, for rapid post-fire assessments and emphasize the need for lithology-specific conservation strategies. Full article

Around the world, a large number of stone artifacts have been exposed to air for long periods of time, showing multiple types of deterioration that have attracted widespread attention. Among them, there is an often overlooked deterioration of stone artifacts, i.e., black stains on the surface of the calcareous stone, which are tightly bonded to the substrate as a result of the long-term deposition of air pollution. However, due to the current lack of a clear understanding of the black stains, people often tend to use the wrong cleaning and conservation methods, which is not conducive to sustainable conservation. Therefore, there is an urgent need to comprehensively recognize the black stains in terms of material properties and environmental sustainability to guide scientific sustainable conservation methods. To this end, in this paper, we take the black stains observed on marble buildings in the Xianling Tomb, China, as an example, and for the first time, we aim to create a comprehensive understanding of black deposition from the aspects of material properties and environmental characteristics. Multi-analytical approaches, including polarized light microscopy, X-ray fluorescence (XRF), and scanning electron microscopy with energy dispersive X-ray spectrometry (SEM-EDS), were employed to discern the differences between the substrate and black stains. The results revealed that the formation of black stains was attributed to prolonged exposure to various air pollutants (PM, SO₂, NO₂, CO, and O₃). Subsequently, observational data from 2015 to 2023 were utilized to investigate the temporal evolution of local air pollutants and their coupled resonances. Multi-scale variations (annual, seasonal, monthly, weekly, and daily) of pollutant concentration sequences were identified, which helps us to have a clearer perception and to proactively control air pollutants in the region from different cycles. In addition, wavelet coherence (WTC) demonstrated significant time-scale dependency in correlation with air pollutants, which provides effective data support for the coordinated control of air pollutants. This study reveals the mechanism of black stain deterioration on stone artifact surfaces, provides data support for the control and prediction of air pollutants oriented to the sustainable conservation of stone artifacts, and provides a novel and comprehensive approach to the scientific knowledge and sustainable conservation of stone artifacts. Full article

A diagnostic chemical analysis has been performed on a Roman Cippus funebris in precious white marble located close to an ancient Roman road. The Cippus was in good condition but almost completely covered by a black patina, requiring a conservative cleaning intervention. The restorer in charge of the restoration asked us to make a preliminary diagnosis, on the basis of which we could suggest the most appropriate intervention. The Cippus was dedicated to the young Quintus Cornelius Proclianus, who died at the age of 15, by his mother Valeria Calpurnia Scopele. It perfectly fits into the Roman funerary liturgy and also shows an Etruscan-type iconography that seems to confirm the Etruscan Gens of the family and its dating to the 1st century AD. Ion chromatography (IC) analyses were performed to determine anions and cations on solutions obtained from the extraction of salts from the four samples of the Cippus. pH, conductivity, and red-ox potential measures, as well as UV-visible spectra were carried out on the same solutions. A small fragment, spontaneously fallen from the Cippus’ surface, was also observed by optical microscopy (OM) and scanning electron microscopy (SEM) coupled with energy dispersive spectroscopy (EDS). From the analyses, the dark patina that covered the surface before cleaning turned out to be made of black crusts, that is, smog particles adsorbed on sulfates, but above all, by a layer of microflora. The results allowed us to suggest some conservative interventions. Full article

Despite increasing conservation efforts for shorebirds, there are widespread declines in many shorebird species in North America. Climate change is causing significant shorebird range shifts and population declines. This study investigates the relationship between meteorological variability and shorebird population dynamics over ten years (2014–2023) at Fish Haul Beach, Hilton Head Island, South Carolina, USA. Shorebirds, reliant on specific habitats for breeding and foraging, are increasingly vulnerable to climate-driven changes, including shifts in temperature, precipitation, and wind speed. Using Generalized Additive Models with Poisson distribution, we analyzed species-specific count data for 12 shorebird species in relation to annual meteorological variables. Additionally, the Mann–Kendall test and Sen’s slope were employed to assess decadal trends in population counts. The results reveal significant declines in Black-bellied Plover (Pluvialis squatarola), Marbled Godwit (Limosa fedoa), and Willet (Tringa semipalmata). In contrast, Semipalmated Plover (Charadrius semipalmatus) and Piping Plover (Charadrius melodus) showed increasing trends, indicating potential habitat benefits or conservation success. Temperature emerged as a key driver affecting the abundance of several species, while precipitation and wind speed also played crucial roles in shaping population dynamics. Our findings underscore the sensitivity of shorebird populations to weather fluctuations, emphasizing the need for integrating meteorological variability into management strategies to ensure shorebird conservation. This study provides critical insights into the impacts of meteorological variables on migratory shorebird populations along the Atlantic Flyway. It highlights the importance of maintaining healthy coastal ecosystems in South Carolina. Full article

This work illustrates the results of an archaeometric investigation performed on 29 stone tesserae collected from 2021 to 2024 during the restoration campaigns of three mosaic floors at Praedia Iuliae Felicis in Pompeii (Campania, Italy). The tesserae were studied through a micro-analytical approach using Polarized Optical Microscopy (POM), Scanning Electron Microscopy (SEM) with Energy-Dispersive X-ray Spectroscopy (EDS) and Electron Probe Micro-Analyzer with Wavelength-Dispersive Spectroscopy (EPMA-WDS). The aim of the study was to determine the petro-chemical features of the mosaic tesserae and to obtain information about the provenance of the raw materials. The research highlights the use of different types of stone, such as volcanic and sedimentary, of probable local provenance. A single marble tessera was also recognized. In addition, the archaeometric study allowed a better knowledge of the executive technique and contributed to orienting the technical choices of the restoration. Indeed, a complementary study of conservation state of the three mosaic floors was conducted with the aim of better defining strategies for conservation and restoration, paying attention to the compatibility and to the interaction of the restoration products with the characteristics of the raw materials. Full article

Outdoor stone relics, including inscriptions, statues, temple grottoes, etc., are continuously subjected to natural weathering and air pollutants. Those made of marbles and other carbonate rocks are particularly vulnerable to acid rains, which can be protected by acid-resistant coatings. A novel method to prepare enamel-like hydroxyapatite coating on marble surfaces is presented in this paper and analyzed using optical microscopy, a scanning electronic microscope, grazing incident X-ray diffraction, and nano-indentation. The described coating is composed of tightly arranged hydroxyapatite nanorods, perpendicular to the marble substrate, with a thickness of 3–5 μm. Not only does the coating exhibit high acid resistance, it also has considerably higher elastic modulus and hardness compared to that synthesized by the well-known diammonium phosphate (DAP) method owing to the wellarranged microstructure. Consequently, the enamel-like hydroxyapatite coating would probably be more effective and durable for marble protection than the existing calcium phosphate coating. Full article

A marble processing plant (MPP) can achieve sustainable development by implementing energy-saving and consumption-reduction technology. Reducing energy loss in such an energy-intensive plant is crucial for overall energy savings. This study establishes an MPP optimization model based on the second law of thermodynamics and the law of conservation of mass. Marble is an aesthetically pleasing and long-lasting building material that has boosted economies in European and sub-Saharan African nations. However, high energy costs and scarcity have constrained the industry’s economic potential and hindered the achievement of optimal levels of production. The second law of thermodynamics is adopted to study the irreversibilities, inefficiencies, and exergetic performance of a marble processing plant. The Aspen Plus commercial software application is used to model and generate thermodynamic data, determine energy flow streams and conduct sensitivity and optimization analysis to improve data quality and energetic performance outcomes. From the results, the various scales of the exergetic destruction, efficiencies, and exergetic losses are determined, and recommendations are established. The overall energy and exergy efficiency levels were determined to be 87.43% and 86.84%, respectively, with a total exergetic destruction of 200.61 kW. The reported methodologies, cutting-edge ideas, and solutions will give industrialists and other significant stakeholders in the global manufacturing sector cutting-edge information about energy usage and ways to cut energy losses in both new and existing factory designs, manage energy cost components, and adjust energy efficiency to maximize productivity. Full article