Search Results (52,046)

Sexual dimorphism in morphological traits is widespread across animals and can result from differing life-history strategies, sex-specific competition, and ecological interactions influenced by habitat structure. For epifaunal organisms such as amphipods, habitat complexity mediates access to food, mate encounters, and refuge. This study investigates sex-related variation in body size and gnathopod 2 ratio (gnathopod 2 length/body length) in two amphipod species, Ampithoe ramondi and Caprella acanthifera, across four benthic habitats: rhodolith beds, macroalgae-dominated reefs, seagrass meadows, and black coral forests. A. ramondi occurred in all habitats except black coral forests, with males larger than females only in macroalgae-dominated reefs and exhibiting higher gnathopod ratios, increasing across macroalgae-dominated reefs, seagrass meadows and rhodolith beds. C. acanthifera was found in macroalgae-dominated reefs and black coral forests, with males larger on average but no significant habitat-related variation in dimorphism. These results indicate that sexual dimorphism patterns are species-specific, shaped by habitat-specific ecological pressures and life-history strategies. Expanding such analyses to more taxa and with balanced sampling across habitats and environmental gradients will offer deeper insight into how natural and sexual selection interact and inform how these dynamics may shift under changing climate regimes. Full article

Background: Torture, as a fundamental violation of human rights, is unequivocally condemned by all international organizations. Sexual torture is one of the most severe forms of torture, encompassing forced nudity, various forms of humiliation, and physical abuse, including rape. Despite testimonial evidence indicating the incidental use of sexual torture by Israeli authorities, there is a lack of epidemiological research providing a comprehensive understanding of this issue. This study aims to analyze the prevalence and characteristics of ill treatment and sexual torture among Palestinian male detainees and the subsequent impacts. Methods: This cross-sectional study analyzed a database of 517 former male detainees. The interview protocol included items related to psychological and physical methods of sexual torture, medical impacts, subjective psychological impacts, clinical medical and psychological measures, and psychosocial and community impacts. Results: The findings indicate that the majority of detainees experienced some form of sexual torture, with humiliation being the most common type. The impact of sexual torture are severe, affecting both clinical and social domains. The impacts of sexual torture persist over time and, in some cases, worsen, particularly regarding physical health outcomes. Socially, the consequences extend to the detainees’ families and communities. Conclusions: The prevalence of such torture tactics calls for urgent responses from both the authorities and civil society. These findings highlight the need for proactive measures to address and mitigate the impacts of sexual torture, including independent investigations, robust monitoring, secure reporting mechanisms, the prosecution of perpetrators and comprehensive reparation for victims. Full article

The genus Siccibacter consists primarily of environmental bacteria, with strains of Siccibacter colletis previously isolated only from plant materials and related environments. This study aims to characterize the first clinical isolate of S. colletis and explore its genomic evolution and clinical relevance. Strain S25242 was isolated from the urine of a 64-year-old male with a severe urinary tract infection. The genome of S25242 is 4.19 Mb, containing 4012 coding sequences, 73 tRNAs, 10 rRNAs, and 38 snRNAs. Phylogenetic and phylogenomic analyses indicated that strain S25242 is closely related to S. colletis type strain 1383^T. The strain shared >70% of digital DNA-DNA hybridization (dDDH) values and >96% of average nucleotide identity (ANI) values with the type strain of S. colletis 1383^T, thereby confirming its taxonomic status. The isolate was susceptible to all 11 tested antimicrobials. Comparative genomics identified 1942 S. colletis-specific genes (including multidrug efflux systems) and 13 unique genes in S25242 related to transposition and DNA integration. This study reports the first clinical isolate of S. colletis, providing evidence that genomic plasticity facilitates its transition from an environmental inhabitant to an opportunistic pathogen. The findings highlight the need for enhanced clinical surveillance of the Siccibacter genus and offer insights into its genomic evolution and clinical adaptation. Full article

Background/Objectives: The objective of this investigation was to assess the prevalence of sarcopenia and identify its related risk factors in patients with rheumatoid arthritis (RA) using data from the nationally representative 2008–2011 Korea National Health and Nutrition Examination Survey (KNHANES). Methods: We analyzed data from the 2008–2011 KNHANES to identify the factors associated with sarcopenia in patients with RA. Sarcopenia was defined as the ratio of appendicular skeletal muscle mass to total body weight (multiplied by 100), with cut-offs of <29.0% for men and <22.9% for women. To identify the specific factors independently associated with sarcopenia, a multivariate logistic regression model was employed, accounting for sample weights and the complex survey design. Results: Among 238 patients with RA included in the analysis, 44 (weighted prevalence: 22.7%) had sarcopenia. The sarcopenia group had a higher proportion of males (55.0% vs. 15.3%, p < 0.001), body mass index (BMI) (26.1 vs. 23.3 kg/m², p < 0.001), and waist circumference (86.9 vs. 79.0 cm, p < 0.001) than the non-sarcopenia group. After adjustment for potential confounders, including age, sex, obesity, physical activity, and daily protein intake, male sex (odds ratio [OR]: 4.17; 95% confidence interval [CI]: 1.48–11.77, p = 0.007) and obesity (OR: 3.06; 95% CI: 1.16–8.07, p = 0.024) were independently associated with sarcopenia. In sex-specific analyses, low physical activity was significantly associated with sarcopenia only in male patients (OR: 13.00; 95% CI: 1.90–88.75, p = 0.012). Conclusions: Our findings indicate that being male and having a higher BMI are significant independent indicators of sarcopenia risk within the Korean RA population. This highlights their critical role in the development of sarcopenia among RA patients. Full article

Background/Objectives. Head and neck mucosal melanoma (HNMM) is a rare, aggressive malignancy with poor outcomes and limited evidence to guide prognostication and treatment. A detailed assessment of long-term survival and prognostic factors is needed to inform clinical management and staging. This work aimed to describe outcomes and prognostic factors in HNMM patients treated over 45 years. Methods. This was a retrospective observational cohort study of consecutive patients treated at a tertiary referral center in Italy from 1975 to 2020. Random-forest-based screening informed covariate selection for Cox models. Endpoints were overall survival (OS), disease-free survival (DFS), and post-recurrence DFS (prDFS). Associations with clinical and pathological variables were evaluated using Kaplan–Meier estimates, log-rank tests, and multivariable Cox regression. Results. Among 112 patients (median follow-up, 121.1 months), 3-/5-year OS was 42.8%/28.0%, DFS 20.5%/13.2%, and 1-/3-year prDFS 36.7%/10.9%. Ulceration was associated with worse OS (HR 2.12; 95% CI 1.05–4.26) and DFS (HR 2.23; 95% CI 1.16–4.28). Male sex showed a trend toward poorer OS and DFS. Regional lymph-node treatment correlated strongly with OS and prDFS (overall p < 0.001), with neck dissection indicating unfavorable risk (OS HR 5.22; 95% CI 2.39–11.40). Conclusions. HNMM remains a high-mortality disease with frequent recurrence. Ulceration and nodal involvement were key adverse prognostic factors, while surgery was associated with improved survival. The findings support incorporating ulceration into future staging and highlight the potential for durable control through salvage surgery. Further investigation of treatment intensification, biomarkers, and multimodal strategies is warranted. Full article

This study examined how youth sport coaches support positive youth development (PYD) through their pedagogical practices, focusing on the following key PYD outcomes: Competence, Confidence, Contribution, and Character. A total of 113 youth sport coaches were systematically observed, involving athletes aged between 12–18 years. The coaches (M = 38.64 years; Male = 74; Female = 39) represented both individual and team sports and had an average coaching experience of 12.92 years. Our results showed that coaches put the most effort on Competence, followed by Confidence, while Character and Contribution received comparatively less attention. Competence development was mainly supported through verbal instruction, Confidence through general feedback, Contribution through active listening, and Character through role modeling. Regarding sport types, coaches in individual sports demonstrated higher levels of Competence and Contribution than team sport coaches. Furthermore, all four PYD dimensions were positively correlated, suggesting that gains in one dimension may support the others. Overall, Hungarian coaches emphasize technical and tactical competence, with less focus on social skills and value development. Confidence is mainly supported through general rather than specific feedback. Coaches in individual sports showed higher PYD engagement than team sport coaches. These findings highlight the need for more balanced, developmentally intentional coaching approaches. Full article

Monosex all-male culture of the giant freshwater prawn (Macrobrachium rosenbergii) maximizes aquaculture yield due to a male growth advantage, but direct hormonal treatment of grow-out populations poses significant food safety risks. This study evaluated the efficacy of dietary 17β-estradiol (E2) in inducing functional neo-females from a fully all-male postlarval population to support an indirect monosex seed production strategy. All-male postlarvae were fed diets supplemented with E2 at concentrations of 0, 50, 100, 150, and 200 mg/kg for 36 days, followed by a 150-day hormone-free post-treatment period to assess growth performance, feminization rates, and gonadal histology. E2 administration successfully induced feminization across all treatments, reaching a peak rate of 35.5% at 150 mg/kg, whereas the control group remained entirely male. During the 36-day treatment period, E2 supplementation transiently enhanced specific growth and survival rates but concurrently reduced feed conversion ratios. Notably, these physiological differences disappeared completely over the 150-day post-treatment phase. Histological assessments confirmed that E2-induced neo-females exhibited normal oogenesis, with gonadosomatic index (GSI) values and oocyte diameters similar to those of wild-caught females. This establishes a definitive, physiologically safe, and non-surgical protocol for producing the neo-female broodstock necessary to sustain high-yield commercial monosex populations. Full article

Background: Oral lipomas are uncommon benign tumors composed of mature adipocytes, accounting for roughly 1% of benign intraoral lesions. Common predilection sites are buccal mucosa, lips, and tongue, presenting as slow-growing, nodular masses, often with a yellow hue. As the size of most lesions does not exceed 10 mm, particularly larger lipomas may be misdiagnosed. We present two cases of large oral lipomas. Case reports: Case 1: A 58-year-old male with a painless, sessile nodular mass of approximately 2.5 cm in the left cheek, increasing in size and causing discomfort during mastication. After excision, histopathology revealed mature adipocytes with delicate fibrous septa. Case 2: A 47-year-old female with a tender yellow growth of approximately 2 cm in her lower lip, increasing in size and causing aesthetic problems with functional discomfort. After sharp dissection, the specimen presented acanthotic and parakeratotic epithelium with adipocytic tumorous tissue, permeated by collagenous cords. Conclusions: Oral lipomas are uncommon, mostly asymptomatic benign lesions. Mostly found in the buccal mucosa and lower lip, they can mimic more common growths. When located superficially, a conservative surgical excision leads to resolution with rare recurrences. Histopathological inspection is necessary to confirm the benign nature of the lesion. Full article

Background/Objectives: Phthalates are a group of organic compounds widely used for enhancement in flexibility and transparency of polyvinyl chloride (PVC) products. Exposure to phthalate-containing substances has been shown to affect brain function, particularly in learning and memory processes. Quercetin is a plant-derived flavonoid with remarkable anti-oxidant and anti-inflammatory potential. This study investigated the possible protective effects of quercetin on spatial learning and memory, histomorphometric changes, and hippocampal expression of inflammatory cytokines (TNF-α and IL-6) in male mice exposed to di(2-ethylhexyl) phthalate (DEHP). Methods: A total of 42 male mice were divided into seven groups. Quercetin was administered orally at doses of 25 and 50 mg/kg/day, either alone or in combination with DEHP (200 mg/kg/day). Following the final day of the treatment, spatial learning and memory were assessed by the Morris Water Maze test. Hippocampal tissues were sampled for Nissl, H&E, and immunofluorescence staining. Quantitative real-time PCR was used to measure the expression of TNF-α and IL-6. Results: The DEHP group exhibited significant impairments in learning and memory, neuronal damage, and cellular disorganization in the hippocampus, along with increased astrocyte activation and elevated expression of TNF-α and IL-6. On the other hand, quercetin supplementation significantly reduced these inflammatory markers and histological damages and also improved spatial learning and memory. Conclusions: Overall, quercetin improves cognitive function that is associated with attenuating astrocyte activation and inflammation. Full article

Background: The dorsal raphe nucleus (DRN) produces and distributes serotonin, while the hypothalamus (HYP) uses serotonergic signals to regulate physiological processes in chickens. Coconut oil (COCO), rich in medium-chain fatty acids, is rapidly absorbed without re-esterification. Methods: Day-old broilers (Ross 708 male, n = 160) were distributed into two dietary treatments with five replicates of 16 birds each. The birds were fed a corn–soybean meal (SBM) basal diet supplemented with 3% of poultry fat (CON) or coconut oil (COCO). The body-weight gain (BWG), feed intake (FI), and feed conversion ratio (FCR) were recorded over a 3-week period, and the data were subjected to a t-test. Untargeted metabolomic analysis by high-performance liquid chromatography (HPLC-MS) was used to evaluate the influence of the type of dietary fat on metabolite profiles in the DRN, HYP, and plasma of broiler chickens. Principal component analysis (PCA) was used to identify unique metabolites, and ANOVA was used to identify the metabolites that were significantly abundant (p < 0.05). The metabolites were then annotated using the KEGG and HMDB databases. Results: Birds in the COCO treatment gained more weight on average (0.8446 kg/bird) than birds in the CON group (0.8132 kg/bird; p = 0.0496). Five metabolites associated with multiple significant cellular processes, such as brain function, energy metabolism, and neurotransmission, showed similar differential expression patterns, while two metabolic pathways (butanoate metabolism and alanine, aspartate and glutamate metabolism) were identified. Conclusions: The dietary inclusion of COCO improves BWG in poultry and enhances their overall well-being by modulating metabolite profiles, supporting neurotransmission, and enriching the metabolic pathways essential for growth and brain function. Full article

Background and Objectives: The double burden of malnutrition (DBM), characterized by the coexistence of malnutrition and overweight within the same household, has become a significant public health concern in low- and middle-income countries. Tanzania is undergoing a nutritional transition marked by persistent child malnutrition alongside increasing maternal overweight. This study examined socio-demographic, maternal, and child-level factors associated with DBM among children under five years in Tanzania. Methods: This cross-sectional study used data from the 2022 Tanzania Demographic and Health Survey, including a weighted sample of 5744 children under five and their mothers aged 15–49 years. DBM was defined as the presence of child malnutrition, measured using the Composite Index of Anthropometric Failure (CIAF), in households where the mother was overweight or obese. Bivariate chi-square tests and binary logistic regression analyses were conducted in STATA 17. Adjusted odds ratios (AORs) with 95% confidence intervals (CIs) were estimated to identify predictors of DBM. Results: DBM was more prevalent in rural areas. Significant predictors included birth order (AOR = 0.611, p = 0.030), child sex (AOR = 0.708, p = 0.011), perceived birth size (AOR = 0.270, p = 0.004), child age (AOR = 0.474, p < 0.001), maternal age (AOR = 0.599, p = 0.045), and maternal education (AOR = 0.604, p = 0.035). Higher maternal education reduced the likelihood of DBM, while firstborn male and small-sized children were at greater risk. Conclusions: DBM in Tanzania is influenced by both biological and socio-demographic factors. Integrated, multi-sectoral interventions targeting maternal education, prenatal care, and optimal maternal nutrition are essential to reduce DBM and achieve global malnutrition reduction targets. Full article

Background/Objectives: Cholinergic dysfunction plays a central role in memory impairment, yet trihexyphenidyl (THP)-based paradigms remain less explored than scopolamine-based models. This study aimed to characterize a THP-induced cholinergic challenge in a two-trial Y-maze with a 24 h interval and to compare the effects of donepezil and tacrine on recall-phase exploratory allocation. Methods: Male Wistar rats (n = 9/group) were studied in a validation phase including saline, THP 5 mg/kg, and THP 10 mg/kg groups, followed by an intervention phase including control, THP 10 mg/kg, donepezil 1 and 3 mg/kg + THP, and tacrine 3 and 5 mg/kg + THP groups. All treatments were administered intraperitoneally (i.p.). Acquisition- and recall-phase behavior was analyzed. Recall outcomes included arm times, arm entries, the novel-to-familiar arm time ratio (U/K time ratio), the novel-to-familiar arm entry ratio (U/K entry ratio), discrimination indices and time-per-entry indices. Data were analyzed by one-way ANOVA; Tukey’s post hoc test was used in the validation experiment, whereas Dunnett’s test was used in the intervention experiment for comparisons against THP 10. Results: THP at 10 mg/kg produced a robust recall-phase phenotype, with increased familiar-arm exploration, reduced novel-arm exploration and lower normalized indices. Under THP challenge, donepezil was associated with clearer effects at 3 mg/kg, whereas tacrine displayed a broader dose-dependent profile, with the strongest shift in recall-phase exploratory allocation toward the novel arm observed at 5 mg/kg. Conclusions: THP 10 mg/kg produced a robust recall-phase exploratory phenotype in a 24 h two-trial Y-maze paradigm. Under THP challenge, donepezil and tacrine were associated with shifts in recall-phase exploratory allocation. These findings support the potential utility of THP-based paradigms for studying cholinergic disruption in Y-maze settings, while direct comparison with scopolamine-based models remains to be established. Full article

Background: Corneal sensitivity is a key indicator of ocular surface health. This prospective, cross-sectional study evaluated agreement between corneal sensitivity thresholds obtained from equivalent stimulus settings on a contemporary, enhanced dual-temperature non-contact corneal aesthesiometer (NCCA) and a previously validated (standard) device. Methods: Central corneal sensitivity thresholds were measured in the right eyes of healthy participants using both devices. Participants with previous ocular surgery, laser treatment, trauma, contact lens wear, diabetes, or peripheral neuropathy were excluded. Sensitivity thresholds were determined using a forced-response, double-staircase protocol. Inter-device agreement was assessed using Bland–Altman analysis, and consistency was assessed using intraclass correlation coefficients. Results: Median corneal sensitivity thresholds in 51 healthy participants (32 female, 19 male; mean age: 33 ± 14 years) did not differ between enhanced (0.23 [0.18 to 0.38]) and standard (0.25 [0.15 to 0.35]) NCCA instruments (p = 0.73). Bland–Altman analysis demonstrated moderate inter-device agreement, with a mean difference of −0.01 mbar (95% limits of agreement: −0.41 to 0.39 mbar). Linear regression analysis identified greater measurement discrepancies at higher thresholds (p < 0.05), indicating greater variability in individuals with reduced corneal sensitivity. Conclusions: The enhanced NCCA yields reliable corneal sensitivity measures for a room-temperature stimulus and acceptable agreement with the existing (standard) model. Full article