Search Results (16,866)

The present work set out to examine the antidiabetic capacity of Abelmoschus esculentus (okra) fruit extract through a combined experimental and computational framework. Enzyme inhibition assays were carried out against four metabolic targets, and IC₅₀ values stood at 7.66 ± 0.31 mg/mL for alpha-glucosidase, 5.21 ± 0.18 mg/mL for alpha-amylase, 2.11 ± 0.15 microg/mL for DPP-4, and 9.17 ± 0.54 mg/mL for pancreatic lipase. The extract showed moderate-to-weak activity relative to standard inhibitors acarbose, sitagliptin, and orlistat. Sixteen drug-like phytochemicals obtained from the IMPPAT 2.0 database were docked against the crystal structures of all four tested enzymes (PDB: 8CB1, 5E0F, 2ONC, 1LPB). Alpha-Carotene, Vitamin E, and Spiraeoside emerged as the top-ranked compounds across all targets, with alpha-Carotene recording the strongest binding affinity of −11.1 kcal/mol against pancreatic lipase, which was 4.2 kcal/mol more negative than the positive control orlistat (−6.9 kcal/mol). PLIP-based interaction profiling mapped out hydrogen bonds, hydrophobic contacts, pi-stacking, and salt bridges at the atomic level. Absorption, distribution, metabolism, and excretion (ADME) and toxicity screening of alpha-Carotene returned a favourable pharmacokinetic profile with predicted LD₅₀ of 1510 mg/kg (Class 4) and inactivity across most toxicity endpoints. A 100 ns molecular dynamics simulation of the pancreatic lipase-alpha–Carotene complex, alongside the orlistat control, showed stable root mean square deviation (RMSD) (0.15–0.22 nm), a consistent Rg (~1.97 nm), and sustained hydrogen bonding throughout the trajectory. Free-energy landscape analysis revealed a well-defined single energy basin for alpha-Carotene, suggesting a thermodynamically stable binding conformation. These findings lay the molecular basis for using okra phytochemicals as adjunctive agents in diabetes management, though in vivo validation remains necessary. Full article

Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease and a global public health concern, for which there is currently no effective method to inhibit its progression. The pathogenesis of NAFLD is related to hepatic lipid metabolism disorders and liver inflammation. Previous studies have shown that tea polysaccharides (TPS) have the ability to regulate lipid metabolism and control inflammation. This study aimed to observe the effect of TPS on ameliorating NAFLD in a mouse model and to reveal its underlying mechanisms. In the current study, male C57BL/6J mice were fed a high-fat diet and administered 100 mg/kg TPS daily by gavage for 14 weeks. Then, liver injury indicators and macrophage polarization markers were detected. The results revealed that TPS could significantly ameliorate the progression of NAFLD and decrease liver injury indicators. Moreover, we found that treatment of NAFLD model mice with TPS could skew liver macrophages polarization from M1 to M2 type, which inhibited pro-inflammatory cytokines production and liver inflammation. Mechanistically, TPS cannot directly regulate the polarization of liver macrophages, but instead promotes the production of butyric acid by gut microbiota, which in turn regulates macrophage polarization. These findings suggest that TPS ameliorates NAFLD-associated inflammation by modulating the gut–liver axis and promoting M2 macrophage polarization, laying the foundation for the potential of TPS in the development of health foods for NAFLD. Full article

Objectives: Prostate cancer is the most frequently diagnosed cancer among men, with Latino patients experiencing higher cancer-specific mortality compared to non-Hispanic populations. We evaluated prostate cancer outcomes across distinct Latino subpopulations. Methods: We retrospectively reviewed a single tertiary comprehensive cancer center database of patients with localized prostate cancer treated with radical prostatectomy from 2003 through 2020. Latino patients were subcategorized as Mexican, South/Central American (SCA), or Caribbean (Puerto Rico and Cuba). Non-Hispanic White (NHW) patients served as the reference group. Results: Overall, 7084 patients were included, of which 78% (n = 5518) were NHW and 10% (n = 679) were Latinos. Compared to NHW, Latino patients were younger at the time of surgery (median 62 vs. 64 years) and had higher median BMI (median 28.8 vs. 27.5 kg/m²), median baseline PSA (6.2 vs. 5.5 ng/mL), and D’Amico intermediate- (44% vs. 42%) and high-risk disease rates (16% vs. 13%) (all p < 0.001). Among Latino patients, Mexican patients had higher median baseline PSA (6.7 vs. 6.1 vs. 6.1 ng/mL, p = 0.005) compared to SCA and Caribbean patients. Survival analysis revealed shorter 5-year (97% vs. 99%) and 10-year (89% vs. 96%) overall survival (OS) rates in Mexican Latinos compared to non-Mexican Latinos (both p < 0.05). The 5-year (77% vs. 80% vs. 95%) and 10-year (63% vs. 65% vs. 86%) biochemical recurrence-free survival rates were shorter for Mexican patients compared to SCA and Caribbean patients. On multivariable analysis, SCA subpopulation status was associated with significantly better OS, and pathologic T stage > T2 was associated with worse OS. Conclusions: We identified significant differences in prostate cancer outcomes for different Latino subpopulations, underscoring the importance of population disaggregation in this ethnic group. Full article

Background: This study investigated the effects of melatonin (50 mg/kg/day) and a high-monounsaturated-fatty-acid (MUFA) diet (60% MUFAs; PUFA/SFA ratio = 5), alone or combined, on lipid metabolism-related gene expression in female Sprague–Dawley rats. Methods: Thirty-two eight-week-old rats were randomized into four groups: control (C), melatonin (M), experimental oil diet (E), and combined melatonin plus experimental oil diet (ME). Treatments lasted eight weeks, with melatonin given orally at 17:00–19:00 daily. Results: In the ME group, hepatic expression of peroxisome proliferator-activated receptor α (PPARα) and acyl-CoA oxidase (ACO) mRNA was markedly upregulated, while the E group showed significantly higher expression of hormone-sensitive lipase (HSL) in white adipose tissue. Additionally, ME rats exhibited increased fibronectin type III domain-containing protein 5 (FNDC5) mRNA with reduced peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), but serum irisin remained unchanged. Conclusions: Melatonin and a high-MUFA diet induced modest and non-uniform changes in lipid metabolism-related gene expression. However, these transcriptional responses were not accompanied by measurable physiological or metabolic outcomes, indicating that the observed effects represent early molecular adaptations rather than functional metabolic modulation. Full article

Background: Hypothyroidism is one of the most common endocrine conditions during pregnancy and has been associated with poor obstetric and perinatal outcomes. There is still a lack of data from Middle Eastern populations, despite its clinical significance. This study aimed to evaluate thyroid management patterns during pregnancy and examine the association between thyroid function control and maternal and perinatal outcomes in women with hypothyroidism at a tertiary care center in Qatar. Methods: A retrospective cohort study including 379 pregnant women with hypothyroidism diagnosed between January 2019 and November 2022 was conducted at Sidra Medicine in Doha, Qatar. Based on trimester-specific Thyroid-stimulating hormone (TSH )reference values, participants were categorized as having adequately or inadequately controlled thyroid function. Data on obstetrics, biochemistry, and demographics were taken from electronic medical records (EMR). Statistical analyses were performed using chi-square tests for categorical variables and t-tests for continuous variables, with a significance threshold of p < 0.05. Results: Participants had a mean Body Mass Index (BMI) of 30.33 ± 6.14 kg/m² and an average age of 32.65 ± 4.99 years; 54% of them were Qataris. Of the patients, 58.5% had positive thyroid antibodies and 55.7% had pre-gestational hypothyroidism. Women with pre-gestational hypothyroidism required significantly higher levothyroxine doses compared with those with gestational hypothyroidism (93.2 ± 47.5 mcg/day vs. 67.6 ± 30.1 mcg/day; p < 0.001). Treatment adjustment was demonstrated by the improvement in TSH normalization from 51.3% in the first trimester to 64.2% in the third trimester (p = 0.041). No significant associations were observed with pre-eclampsia, preterm delivery, hypertension, or placental abruption. However, women with normal third-trimester TSH had a higher prevalence of gestational diabetes mellitus (GDM) compared with those with elevated TSH (51.6% vs. 36.8%; p = 0.013). Conclusions: Appropriate trimester-specific monitoring and timely levothyroxine titration was associated with improved biochemical control without adverse maternal outcomes. Greater levothyroxine requirements in women with pre-gestational hypothyroidism emphasize the importance of early intervention. These findings highlight the potential benefit of structured thyroid monitoring and multidisciplinary care approaches in pregnancy and may help inform future regional clinical practice guidelines. Full article

Since 2021, the design and construction of nearly zero-energy buildings (nZEBs) have been mandatory for European Union Member States. Subsequent requirements for the building sector, characterized by high energy demand and significant environmental impact, include the minimization of carbon footprint and the introduction of climate-neutral building standards. The carbon footprint comprises both embodied emissions related to materials and construction processes and operational emissions resulting from building use. This paper analyzes both types of carbon footprint using a residential building that is part of an experimental housing estate consisting of 44 semi-detached buildings as a case study. Analyses of energy consumption optimization and carbon footprint reduction were conducted at both the individual building scale and the scale of the entire housing complex. The estate was developed in two stages. In the first stage (completion of construction in 2024), the primary criterion for technology selection was investment cost while maintaining compliance with applicable technical and building regulations. Prior to the implementation of the second stage, the investor conducted a social participation process in the form of a survey among future users. The survey addressed environmental aspects of the newly designed buildings and enabled the selection of materials, technologies, and energy sources aligned with user preferences. The results indicate that environmental aspects are important to future users; however, investment decisions are strongly balanced against economic factors. At the same time, the energy analyses demonstrate that a substantial reduction in the operational carbon footprint can be achieved, enabling a significant progression toward climate neutrality, both at the level of individual buildings and across the entire housing estate. Social participation, therefore, becomes an important element in the pursuit of climate neutrality in buildings. However, it must be taken into account already at the design stage. The results of the analyses carried out in the article showed that, taking into account public participation in the design process and user recommendations, the selected optimal variant (W5) allows for a reduction in the EP index by over 90% compared to the variant based on standard low-cost solutions (W0) (EP (W0) = 243.64 kWh/(m² year); EP (W5) = 18.42 kWh/(m² year). In terms of the embodied carbon footprint, the optimal option W5 allows for a reduction of over 30% in the embodied carbon footprint of the building structure (W0—51,585.32 [kgCO₂e]; W5—35,537.87 [kgCO₂e]). The optimal variant indicated by users (W5) allows for a reduction in the operational carbon footprint by approximately 80% compared to the basic variant (W0): W0—604,189.50 [kgCO₂e/kWh]; W5—247,402.0 [kgCO₂e/kWh]. The results obtained indicate that public participation is not only a complementary element of the design process, but it can also be a key component of the decarbonisation strategy in residential construction. Involving future users in the decision-making process increases the likelihood of achieving long-term greenhouse gas emission reductions and supports the implementation of long-term climate policy goals. Full article

Soft actuators provide a wide range of motion capabilities, allowing for the advancement of novel mobile robots. However, soft actuators that possess the capability required to achieve three-dimensional movement are limited. In addition, the presence of air supply tubes poses a challenge to utilizing pneumatic actuators as mobile robot components. This study presents a long inflatable actuator with a novel structure in which air supply tubes are arranged within its body. This structure enables the extension of the inflatable tube with minimal deformation. The proposed actuator comprises an inflatable tube and a spool mechanism. The length of the actuator is controlled by a motor. The performance of the actuator was evaluated experimentally, validating its alignment with our proposed models. The results showed that the proposed actuator exerted extension and contraction forces of 28 N and 87 N, respectively. Furthermore, the proposed actuator can be equipped with a gripper at its tip, enhancing its functionality. In a demonstration, this gripper-equipped actuator successfully extended to grasp a bar at a height of 1.3 m and contracted while lifting a 1.0 kg base. This demonstration indicated that the proposed actuator could provide the required arm motions of a bi-arm climbing robot. Full article

Background: Ultimate Frisbee (UF) is an intermittent team sport with distinct positional roles (cutters and handlers), yet evidence integrating anthropometric, body composition, and physical performance profiles by playing position remains limited. This study aimed to examine positional differences in these variables among male UF players. Methods: Forty male players (age: 25.13 ± 3.76 years; 7.0 ± 2.5 years of training experience) participated in this cross-sectional design, including 20 cutters and 20 handlers. Anthropometry, body composition, and dynamic balance variables were analyzed using independent-samples t-tests or Mann–Whitney U tests, as appropriate. Positional differences in somatotype and physical performance were analyzed using a one-way multivariate analysis of variance (MANOVA). Results: No positional differences were observed in general anthropometric variables (p > 0.05). However, handlers exhibited higher body fat percentage (14.32 ± 2.37 vs. 11.95 ± 2.45; p = 0.028), fat mass (11.08 ± 2.51 vs. 8.95 ± 2.67 kg; p = 0.049), and endomorphy (4.15 ± 1.22 vs. 2.99 ± 1.30; p = 0.002) than cutters. In contrast, cutters demonstrated higher speed (20 m sprint: 3.11 ± 0.17 vs. 3.21 ± 0.15 s; p < 0.05), agility (10.16 ± 0.69 vs. 10.69 ± 0.61 s; p < 0.05), and vertical jump performance (Counter Movement Jump: 40.93 ± 6.54 vs. 36.38 ± 4.71 cm; p < 0.05; Abalakov: 46.39 ± 7.88 vs. 40.20 ± 4.68 cm; p < 0.01). No differences were found in intermittent endurance (Yo-Yo Intermitent Recovery Test1): 982 ± 354 vs. 940 ± 348 m), upper-limb power, or dynamic balance. Conclusions: These findings indicate that playing position in UF is characterized by distinct body composition and lower-limb neuromuscular performance profiles, whereas intermittent endurance, upper-limb power, and balance represent shared physical requirements across positions. Full article

Background: Overweight and obesity are emerging public health concerns among young adults. However, evidence on associated sociodemographic, dietary, and lifestyle factors among young industrial workers in low- and middle-income countries remains limited. This study aimed to identify factors associated with overweight and obesity among Vietnamese young industrial workers aged 18–30 years. Methods: A cross-sectional study was conducted among 2295 young industrial workers (55.6% men and 44.4% women) recruited from factories and industrial zones in three geographic regions of Vietnam. Sociodemographic characteristics, dietary habits, lifestyle behaviors, and physical activity were assessed using a structured questionnaire. Body mass index (BMI) was calculated from self-reported height and weight and classified using WHO Western Pacific Region (WPRO) cut-offs; overweight/obesity was defined as BMI ≥ 23.0 kg/m². Physical activity was assessed using the International Physical Activity Questionnaire—Long Form (IPAQ-LF) and categorized by total MET-min/week according to IPAQ scoring guidelines. Logistic regression analyses were performed to estimate crude and adjusted odds ratios (ORs) with 95% confidence intervals (CIs). Results: Overall, 10.4% of participants were overweight (BMI 23.0–24.9 kg/m²) and 7.0% were obese (BMI ≥ 25.0 kg/m²), yielding a combined prevalence of 17.4%. After multivariable adjustment, increasing age (aOR = 1.15; 95% CI: 1.10–1.20), male sex (aOR = 2.10; 95% CI: 1.59–2.76), and regular alcohol consumption (aOR = 1.37; 95% CI: 1.04–1.81) were independently associated with higher odds of overweight/obesity, while residence in the Southern region was inversely associated (aOR = 0.57; 95% CI: 0.42–0.76). High total physical activity (vs. low activity) was positively associated with overweight/obesity, whereas moderate physical activity was not independently associated. Other dietary behaviors were not significantly associated after adjustment. Conclusions: Among Vietnamese young industrial workers, overweight and obesity were associated with age, sex, alcohol consumption, and geographic region. The observed association with high total physical activity likely reflects the occupational context of physical activity in this population, highlighting the importance of distinguishing between occupational and leisure time physical activity when interpreting physical activity obesity relationships. These findings underscore the relevance of early, workplace relevant prevention strategies targeting modifiable behaviors, particularly alcohol use. Full article

Background/Objectives: Recent guidelines have emphasized the importance of early mobilization and rehabilitation of patients following cardiac surgery. However, studies on the optimal targets and prescription methods for phase I cardiac rehabilitation (CR) are lacking. This study aimed to evaluate the feasibility and utility of an early phase 1 submaximal cardiopulmonary exercise test (CPET) using a recumbent ergometer in patients who have undergone cardiac surgery. Methods: Twenty ambulatory patients who underwent cardiac surgery between December 2021 and February 2023 were referred to the CR department on the fifth postoperative day, and a CR program was initiated. The program was conducted five times a week, with hour-long sessions consisting of warm-up exercises, resistance training, aerobic exercises, and a cool-down period. A recumbent ergometer-based submaximal CPET was performed approximately nine days after the surgery, prior to discharge. Participants initiated the test at 0 W, and the workload was increased by 20 W after 2 min. During the test, researchers evaluated parameters including submaximal peak values of oxygen consumption (VO₂), metabolic equivalents of task, respiratory exchange ratio (RER), blood pressure, heart rate (HR), and rating of perceived exertion (RPE). The grip strength test, 6 min walk test (6MWT), Korean Activity Scale/Index (KASI), EuroQol-5 dimension (EQ-5D), and short-form 36-item health survey (SF-36) values were also measured prior to discharge. Results: Twenty patients (75% male, average age 62.50 ± 1.99 years) underwent CPET at a median of 9.0 (8.0; 12.5) days postoperative. The average exercise duration of the CPET was 411.75 ± 168.25 s. During the test, their submaximal peak VO₂ was 12.32 ± 0.75 mL/kg/min (corresponding to 46.65 ± 2.08% of VO₂ max). The submaximal peak RER was 1.01 (0.98–1.12), and the submaximal peak RPE was 15.00 ± 0.51. Furthermore, the submaximal peak HR was 111.8 ± 3.76 beats/min (equivalent to 70.95 ± 2.09% of age-predicted maximal HR). After adjustment for age and sex, statistically significant positive correlations were observed between the submaximal peak VO₂ and 6MWT, squat endurance test, KASI, EQ-5D, and the physical component summary (PCS) of the SF-36 questionnaire. The 6MWT, squat endurance test, KASI, and PCS of SF-36 showed a correlation coefficient (r) of 0.522 (p = 0.026), 0.628 (p = 0.005), 0.586 (p = 0.011), and 0.546 (p = 0.019), respectively. No significant cardiac events, such as ST elevation/depression or hemodynamic instability, were observed during the test. Conclusions: Our findings suggest that performing recumbent ergometer-based CPET during early phase 1 CR is safe and feasible. These results highlight the potential of recumbent ergometer-based CPET as a valuable tool for guiding the appropriate prescription of early CR programs following hospital discharge in patients undergoing cardiac surgery. Full article

In this study, the contamination of two drinking water catchments in Australia by per- and polyfluoroalkyl substances (PFAS) was investigated. PFASs in water and sediment were found at hazardous concentrations in waterways affected by transport accidents 24 and 33 years earlier. The exact cause(s) of the PFAS pollution remains unclear due to large data gaps. Both locations experienced burning fuel tankers suppressed using PFAS foam. PFAS contamination of a Blue Mountains water supply triggered the closure of two drinking water reservoirs 3–5 km downstream of the accident site. PFAS contamination of Central Coast’s Ourimbah Creek was concentrated in two floodplain wetlands adjacent to the accident site. The Ourimbah PFAS-affected wetlands are within 500 m of a drinking water groundwater bore field and 1.2 km from a raw water offtake used as part of Central Coast’s drinking water supply. The Blue Mountains contamination has impaired the Blue Mountains World Heritage Area, with perfluorooctane sulfonate (PFOS) exceeding aquatic ecosystem protection guidelines by 100 times. The mean PFOSs in stream water near the area of the Blue Mountains road accident were 2.16 µg L⁻¹ and 213.3 µg kg⁻¹ in stream sediment. This research demonstrates how spillages of small quantities of PFASs can cause major harm due to their extreme persistence, and their levels have exceedance of environmental and health guidelines for decades, with major adverse implications for drinking water supplies and conservation areas. Full article

Preclinical imaging has recently been expanded through the use of ostrich embryos as an alternative in vivo model. In ovo experiments represent a promising substitute for conventional rodent-based animal testing. For artifact-free dynamic nuclear medicine imaging, reliable immobilization of embryos is essential. Although previous studies have demonstrated the feasibility of isoflurane anesthesia, the kinetics and uptake mechanisms of isoflurane in ostrich embryos remain insufficiently characterized. The aim of this study was to characterize gas exchange dynamics in ostrich eggs and to quantify isoflurane uptake using two complementary approaches: indirect consumption measurements in a closed system and direct quantification by serial blood sampling. Fourteen ostrich eggs, including seven fertilized and seven unfertilized specimens, were analyzed at developmental stages up to day 37 of incubation. Gas exchange was assessed in a sealed container using a clinical anesthesia gas monitoring system to measure oxygen consumption and carbon dioxide excretion. Isoflurane uptake was evaluated during exposure to concentrations of 2%, 4%, or 6%. In a separate experimental series, serial blood samples were collected during and after exposure to the same concentrations to determine systemic uptake. Fertilized embryos showed progressive increases in metabolic activity, with a maximal oxygen consumption and carbon dioxide excretion of 116 mL/h/kg and 93 mL/h/kg on day 37. Indirect measurements demonstrated isoflurane uptake rates of up to 1.1 mL/min at 6%, with proportionally lower values at 4% and 2%. Blood analyses confirmed systemic absorption, peak concentrations of 160 µg/mL, and detectable residual levels for up to 120 min after exposure. These findings refine in ovo imaging. Full article

This study investigated the effects of dietary chitosan supplementation at levels of 0, 0.5, 1, 2, 4, and 8 g/kg on the mud crab (Scylla paramamosain) during an eight-week feeding trial. Results revealed significant improvements in final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) as chitosan levels increased, with the 2 g/kg group showing the strongest effects (p < 0.05). Chitosan supplementation also elevated crude protein content in the whole body, muscle, and hepatopancreas (p < 0.05). Moreover, it notably reduced malondialdehyde (MDA) levels in hemolymph and hepatopancreas while enhancing antioxidant enzyme activity (p < 0.05). Chitosan at doses of 1–4 g/kg also significantly increased the activity of non-specific immune enzymes in hemolymph (p < 0.05). Additionally, chitosan enhanced mRNA expression of immune-related genes in the hepatopancreas (p < 0.05). According to gut microbiota study, 2 g/kg chitosan raised the relative abundance of advantageous gut bacteria and modulated microbial composition. In conclusion, appropriate dietary chitosan supplementation successfully stimulates mud crab growth, strengthens immunological and antioxidant systems, and improves intestinal health. Full article

Improving the energy efficiency of the building envelope is critical for global decarbonization, yet a gap remains in the comprehensive thermophysical characterization of carbon-enhanced Expanded Polystyrene (EPS). This study evaluates the impact of expansion ratios and moisture content on the thermal behavior of two commercial EPS grades, EPS-A (12.7 ± 0.5 kg/m³) and EPS-B (16.0 ± 1.1 kg/m³), investigating the counterintuitive role of graphite (1.4–1.8 wt.%) in enhancing the thermal insulation properties. Thermal conductivity and diffusivity were independently determined via Transient Plane Source (TPS) and Heat Flow Meter (HFM) methods across a 10–50 °C range, while specific heat capacity (c_p) was analyzed using HFM and Differential Scanning Calorimetry (DSC) through the sapphire comparison method and Temperature-Modulated DSC (TOPEM^®). Methodologically, it was found that standard HFM protocols are unsuitable for c_p determination in low-density foams, yielding an average relative error of ±29%; conversely, the sapphire comparison method provided the most reliable results in agreement with theoretical expectations. Results indicate that the efficacy of graphite as a radiative shield is closely coupled with cellular morphology, proving significantly more effective in the higher expansion grade (EPS-A, 70 wt.% open porosity) than in the denser EPS-B. Furthermore, 30-day water immersion tests revealed that the higher open porosity of EPS-A facilitates increased water uptake of 144 ± 17 wt.% (compared to 97 ± 7 wt.% for EPS-B), causing the geometric densities of the two grades to converge and fundamentally altering thermal transport mechanisms. The study concludes that accurate thermal modeling of carbon-enhanced insulation requires careful selection of testing parameters, particularly when accounting for moisture-induced degradation in high-porosity systems. Full article

Background/Objectives: Imidazole dipeptides (IDPs), carnosine and anserine, are endogenous antioxidants. The metabolism and functions of IDPs have mainly been investigated in rodents. However, the blood of primates, such as humans, contains carnosinase (CN1), which hydrolyzes IDPs. In non-primates, CN1 is absent, allowing IDPs to be distributed throughout tissues. There are concerns about whether the results of animal experiments can be directly applied to humans. Therefore, we aimed to investigate the blood change in the concentration and tissue distribution of IDPs following their oral administration to golden hamsters, the only non-primates known to possess CN1. Methods: Plasma CN1 activity was compared between hamsters and humans. Hamsters were administered IDPs (an anserine/carnosine mixture) purified from chicken meat at a dose of 1000 mg/kg. Blood samples were collected at time points up to 6 h after administration. Tissue samples were collected at 6 h after administration to measure the concentrations of IDPs and related substances. Additionally, IDP levels in human and mice tissues from previous studies were compared with that of hamster tissues in this study. Results: Hamster plasma CN1 activity was more than 10 times higher than that in humans. Although IDPs were not detected in IDP-treated hamster plasma, constituent amino acids of IDPs increased up to 1–2 h and Nπ-methyl-histidine (m-His) remained at high levels up to 6 h after administration. IDP levels in control tissues (vehicle) were similar to those in human tissues. In the IDP group, tissue IDPs were higher than those in the vehicle and m-His increased in all tissues. Conclusions: This study indicated that m-His levels increase in hamster tissues following a single oral administration of IDPs and strongly suggest that hamsters should be used in functional studies of IDPs in humans, focusing on the functionality of m-His. Full article