Search Results (1,161)

The Wufeng–Longmaxi (WF–LMX) shale gas reservoirs at depths > 3500 m in the Lu203–Yang101 well block, southern Sichuan Basin, possess great exploration potential, but their reservoir characteristics and high-production mechanisms remain unclear. In this study, we employed multi-scale analyses—including core geochemistry, X-ray diffraction (XRD), scanning electron microscopy (SEM), low-pressure N₂ adsorption, and nuclear magnetic resonance (NMR)—to characterize the macro- and micro-scale characteristics of these deep shales. By comparing with shallower shales in adjacent areas, we investigated differences in pore structure between deep and shallow shales and the main controlling factors for high gas-well productivity. The results show that the Long 11 sub-member shales are rich in organic matter, with total organic carbon (TOC) content decreasing upward. The mineral composition is dominated by quartz (averaging ~51%), which slightly decreases upward, while clay content increases upward. Porosity ranges from 1% to 7%; the Long11-1-3 sublayers average 4–6%, locally >6%. Gas content correlates closely with TOC and porosity, highest in the Long11-1 sublayer (6–10 m³/t) and decreasing upward, and the central part of the study area has higher gas content than adjacent areas. The micro-pore structure exhibits pronounced stratigraphic differences: the WF Formation top and Long11-1 and Long11-3 sublayers are dominated by connected round or bubble-like organic pores (50–100 nm), whereas the Long11-2 and Long11-4 sublayers contain mainly smaller isolated organic pores (5–50 nm). Compared to shallow shales nearby, the deep shales have a slightly lower proportion of organic pores, smaller pore sizes with more isolated pores, inorganic pores of mainly intraparticle types, and more developed microfractures, confirming that greater burial depth leads to a more complex pore structure. Type I high-quality reservoirs are primarily distributed from the top of the WF Formation to the Long11-3 sublayer, with a thickness of 15.6–38.5 m and a continuous thickness of 13–23 m. The Lu206–Yang101 area has the thickest high-quality reservoir, with a cumulative thickness of Type I + II exceeding 60 m. Shale gas-well high productivity is jointly controlled by multiple factors: an oxygen-depleted, stagnant deep-shelf environment, with deposited organic-rich, biogenic siliceous shales providing the material basis for high yields; abnormally high pore-fluid pressure with preserved abundant large organic pores and increased free gas content; and effective multi-stage massive fracturing connecting a greater reservoir volume, which is the key to achieving high gas-well production. This study provides a scientific basis for evaluating deep marine shale gas reservoirs in southern Sichuan and understanding the enrichment patterns for high productivity. Full article

Active involvement in creative activities, known as creative health, has been shown to enhance wellbeing, with museums serving as unique spaces for health promotion; however, visitors often require guidance to derive significant benefits from these institutions. This study, part of the larger ASBA (Anxiety, Stress, Brain-friendly museum Approach) project, evaluates the first phase of an intervention specifically focused on a Mindfulness protocol adapted to museum contexts. It has employed a single-group pre–post design with 79 healthy adults recruited from the non-clinical population. Participants were involved in a 15 min standardized mindfulness practice adapted from Mindfulness-Based Stress Reduction (MBSR) in either an art or science museum. State anxiety (SAI) and mood (VAS) were assessed at baseline and post-intervention, alongside personality traits (BFI-10) and interest measures to identify individual moderators of treatment response. The practice appeared to reduce state anxiety significantly in both settings, with large effect sizes. Specific moderators emerged: openness to experience predicted anxiety reduction in the art museum, whereas science interest predicted outcomes in the science setting. These findings suggest that brief, standardized mindfulness protocols implemented through the ASBA framework can provide promising immediate benefits for visitor wellbeing across diverse museum environments. Full article

Large-Format Additive Manufacturing (LFAM) offers the ability to 3D print composites at multi-meter scale and high throughput by utilizing a screw-based extrusion system that is compatible with pelletized feedstock. As such, LFAM systems like the Big Area Additive Manufacturing (BAAM) system provide a pathway for incorporating AM techniques into industry-scale production. Despite significant growth in LFAM techniques and usage in recent years, typical Multi-Material (MM) techniques induce weak points at discrete material boundaries and encounter a higher frequency of delamination failures. A novel dual-hopper configuration was developed for the BAAM platform to enable in situ switching between material feedstocks that creates a graded transition region in the printed part. This research studied the influence of extrusion screw speed, component design, transition direction, and material viscosity on the transition behavior. Material transitions were monitored using compositional analysis as a function of extruded volume and modeled using a standard Weibull cumulative distribution function (CDF). Screw speed had a negligible influence on transition behavior, but averaging the Weibull CDF parameters of transitions printed using the same configurations demonstrated that designs intended to improve mixing increased the size of the blended material region. Further investigation showed that the relative difference and change in complex viscosity influenced the size of the blended region. These results indicate that tunable properties and material transitions can be achieved through selection and modification of composite feedstocks and their complex viscosities. Full article

Background/Objectives: The Seklers are a Hungarian-speaking regional population in Transylvania, Romania, with a long and complex history, yet comprehensive genome-wide studies remain limited. Our aim was to characterize the genetic background of multiple Sekler communities using high-density autosomal data and to place them in a broader Central and Eastern European context. Methods: Here we analyzed genome-wide autosomal SNP data obtained from 17 Sekler groups. Allele frequency- and haplotype-based approaches were applied to assess overall genetic structure, ancestry patterns, recent shared ancestry, and signals of demographic history. Results: Analyses based on overall allele-frequency patterns showed that Sekler groups fit into a single, coherent genetic cluster shared with Hungarians. No major differences were detected among the Sekler communities at this broader genomic level, and their genetic profiles were largely indistinguishable from one another. Using haplotype-based methods, most Sekler groups again formed a compact cluster. However, two villages, Deményháza and Nyárádszentimre, showed clear signs of increased within-group relatedness and subtle separation. These patterns might indicate that both communities experienced stronger local drift and reduced effective population size, while other Sekler groups showed no comparable deviation from the general regional pattern. Conclusions: Although a small number of villages display modest signs of localized demographic drift, our results support that the Seklers represent a regionally distinct and internally cohesive population, whose genetic structure is shaped mainly by common historical and linguistic ties, with minor village-level variation, forming a uniform part of the Hungarian-speaking population of the East-Central European region. Full article

In two-sided disassembly lines, stations are symmetrically arranged on both sides of the conveyor, which is suitable for large-sized waste products. During the disassembly process, evenly assigning parts to workstations while satisfying various constraints and optimizing some disassembly objectives is a challenging task. Therefore, this study deals with a two-sided partial disassembly line balancing problem, and a multi-objective mathematical model for this problem is built. While satisfying various constraints, four objectives, namely, the hazard index, profit, smoothness index, and demand index, are optimized. Due to the NP-hard nature of the problem, an improved discrete whale optimization algorithm is proposed. According to the characteristics of the feasible solutions, an encoding method based on a one-dimensional integer array is designed, which can effectively decrease the memory space and simplify the design of neighbor structures. In the three stages of encircling prey, random wandering, and bubble-net attacking, based on the search features of each stage, different neighbor operators and search strategies are designed to enhance the local exploitation and global exploration capabilities. Finally, the performance of the proposed algorithm was tested against other algorithms for different types of instances and a disassembly case. The results show that the proposed algorithm can not only solve various types of disassembly line balancing problems but also shows superior performance. Full article

Background/Objectives: Optimal fludarabine dosing in the conditioning regimen based on population pharmacokinetic analysis (popPK) can predict outcomes in patients receiving hematopoietic stem cell transplantation. To date, there is no popPK tailored for patients receiving fludarabine as part of the lymphodepleting regimen before chimeric antigen receptor (CAR) T-cell infusion. The objective of this study was to develop a PopPK model of fludarabine in patients receiving CAR T-cell therapy. Methods: A prospective study was conducted at a tertiary hospital, from January 2021 to July 2022. Demographic, clinical, and analytical variables were collected. Blood samples were obtained on days 1 and 3 of the lymphodepleting regimen at 1.5, 2, 7 and 24 h post-fludarabine doses, and 30 min prior to CART-cell infusion. Fludarabine levels were analyzed through an ultra-performance liquid chromatography tandem mass spectrometry assay based on liquid–liquid extraction. Population pharmacokinetic analysis modeling was performed using nonlinear mixed-effects models (NONMEM). Results: Fifty-six patients (59% male) with a median age of 59 years (range 23–82) received CAR T-cell therapy (38 [68%] axicabtagene ciloleucel, 18 [32%] tisagenlecleucel) for relapsed/refractory large B-cell lymphoma. A total of 348 samples were collected for model development. A three-compartment model with first-order elimination best described the data. Body size, as represented by weight (WGT) with allometric scaling, was a significant predictor of all pharmacokinetic parameters (p < 0.05). Estimated glomerular filtration rate (eGFR) and the CAR T-cell construct type also showed statistical significance for fludarabine clearance (CL) (p < 0.05). Clearance was differentiated into non-renal and renal components. Estimates of V1, V2 and V3 volumes (the apparent volume of distribution of the central, shallow and deep compartments) were 41.2, 14.5 and 10.8 L, respectively. Conclusions: WGT, eGFR and type of CAR-T were predictors of fludarabine pharmacokinetics. This model offers a step toward precision-guided lymphodepletion and might support individualized dosing to optimize efficacy and minimize toxicity. Full article

Occupational demands, such as load carriage in tactical professions, do not discriminate based on sex. The aim of this study was to explore the differences in metabolic cost of a loaded pack march between the sexes in both absolute and relative terms. Twelve Army personnel (six males and six females) volunteered to complete three identical load carriage marches (5 km at 5.5 km/h, carrying 30 kg), across flat (on road) and undulating (gravelled path) terrain as part of a larger equipment trial. Heart rate (HR) response (HR average and maximum) was monitored with a Polar Team Pro unit and oxygen consumption with VO Master Pro (VO₂ average and maximum) with the level of significance set at 0.05. There were no significant differences in age, years of experience, absolute loads carried, or completion time for each of the three events. Male soldiers were significantly taller (182.3 ± 6.2 cm vs. 167.4 ± 6.9 cm), heavier (88.2 ± 8.7 kg vs. 70.9 ± 10.6 kg), carried significantly less relative load (34.3 ± 3.4% vs. 43.2 ± 7.5%), and had significantly greater predicted VO_2max (56.7 ± 6.1 mL/kg/min vs. 45.0 ± 2.9 mL/kg/min). A linear mixed model identified a significant main effect of sex on both average HR (β = −1.10) and peak HR (β = −1.27), and on average VO₂ (β = −0.68), but not peak VO₂. While the study was not powered to detect sex differences, the large effect sizes observed suggest meaningful physiological differences warranting further investigation. Female soldiers faced significantly greater metabolic costs when carrying the same loads and moving at the same speed and across the same terrain as their male counterparts. Adequate recovery and pacing strategies should be considered for these events, especially during training. Full article

For large curved parts with complex surfaces, which often exhibit both symmetry and asymmetry in their geometric features, the multi-view combined with the phase shift method and highlight regions treatment method has been proposed and applied to the online measurement system. The hardware components of the measuring system include a self-designed multi-vision platform and a multi-view three-dimensional measurement platform composed of rotating platform, robot and linear guide rail. The overall calibration of the system was conducted to guarantee the effectiveness of the measurement point cloud splicing of each viewing angle. And the system integrates the three-dimensional measurement technology of multi vision combined with the phase shift method and online measure system to realize full coverage and high-precision measurement of the impeller—addressing both its inherent symmetry (regular blade arrangement) and local asymmetry (irregular edge details)—and controls the relative error of the measured size and the actual size within 1%. In addition, the highlight regions treatment method has also been proposed. By adjusting the camera’s exposure time to change the light intensity of the captured images, images under different exposures and their valid pixels are obtained, thereby facilitating the synthesis of a composite image free of highlight phenomena. Experimental results demonstrate that the proposed method can achieve full-coverage measurement of the measured object and effective measurement of highlight regions. Full article

Greater amberjack (Seriola dumerili) are large reef fish important in fisheries in the southeastern USA, with the Gulf of America stock unsustainably harvested over most of the past two decades. Its age-based stock assessment and recovery plan depend on age and growth information. In this study, 7658 greater amberjack were sampled from the west coast of Florida and off Alabama, Mississippi, and Louisiana in the Gulf from 1991 to 2018. Fish were aged using cross-sectioned sagittal otoliths, with accompanying data on their length, sex, location (state), and type of fishery. Overall, the greater amberjack that were landed in the recreational and commercial fisheries were between 2 and 19 years of age, with the majority between 3 and 5 years old (>80%), and were primarily caught using hook-and-line gear (95%). Sex- and state-specific growth differences were evident based on von Bertalanffy growth models, with females significantly larger at age than males in both Florida and Louisiana (which included Mississippi and Alabama due to low sample size), and females in Louisiana larger at age than females in Florida. Sex ratios in the recreational catches of Florida and Louisiana were skewed towards females (>1.5 female per male), especially for fish ≥1000 mm fork length (>2.3 female per male). Accounting for sex-specific and region-specific growth differences may, in part, help to explain the notably high variability in the overall growth model for greater amberjack in the Gulf. Full article

Objective: This study aimed to investigate aspects of sexual response in cisgender women by querying what participants deem to be important physiological and subjective components of sexual arousal. It was also explored whether education about the role of genital blood flow influenced participant responses. Methods: Standard sexuality-based questionnaires were administered assessing function, desire, flexibility, satisfaction, and history, prior to administering a short semi-structured interview. Experiences of sexual arousal were explored in the interview in an open-ended fashion. A brief educational video condition was shared with half of the participants at random. We explored whether being presented additional information about genital function changed the way women describe their physiological response. Those in the control group completed the interview questions once. Those in the educational condition group completed the interview, watched the brief educational video and then repeated the interview questions. Following the interview, all participants completed a brief follow-up questionnaire assessing preferred arousal terminology. Results: The sample size included 30 cisgender women with a mean age of 21.10 (4.38), with 70% in a committed relationship. Additionally, there were no group difference found with those who reported a history of sexual trauma/abuse vs. no trauma/abuse in regard to sexual function, satisfaction, distress and flexibility. The physical arousal sensations described most often in the interview included lubrication (n = 27), blood flow (n = 23), and pulsating (n = 9), whereas, in the ranked term analysis, the three most highly ranked genital sensations were wetness, throbbing and tingling. For non-genital sensations, those most often reported in the interviews included nipples/breast changes (n = 17), skin sensitivity (n = 13), and changes in body temperature (n = 12), whereas them most highly ranked non-genital sensations were heart rate increase, hardness of nipples/breast changes, and skin sensitivity. There were no group differences in the reporting of full-body or genital sensations and the frequency of use of terminology related to blood flow. The two most important themes emerging from the qualitative analysis included the distinct themes of subjective and physiological arousal. The majority of participants placed an emphasis on subjective arousal, deeming it the most important part of their sexual response, with physiological arousal largely deemed important only when subjective arousal was also present. Conclusions: The same themes were present in both groups, indicating that the brief educational video was unlikely to have influenced the way women described their physiological arousal. Participants in both groups placed a dominant emphasis on sensations related to genital blood flow and the importance of subjective arousal. Full article

Solid waste presents a very large problem in the developed world. Waste plastics, which make up a large part of solid waste, have high energy value, which is discarded if they are not treated properly. Most of the plastic found in solid waste is produced from petrochemical material, so it can be used in resource recovery processes to produce various materials. One promising resource recovery process is the pyrolysis process, from which pyrolytic oil, gas, and solid residue are obtained. Pyrolytic oils have properties that are similar to conventional fossil fuels, and are promising fuels for use in heat engines or heating applications. In the present work, HDPE plastic in the form of plastic bottles caps was collected from solid waste and used in a thermal pyrolysis process for the production of pyrolytic oil. The obtained oil was characterised, and the obtained results were compared to conventional fuels. The obtained oil was used further in an oil burner fuel injection application, in which the spray breakup characteristics were monitored and analysed using VisiSize particle characterisation systems. The obtained results were compared to those of conventional fuel. The results indicate that the difference in fuel properties influences the spray breakup process slightly, but the differences are rather small. This indicates that from a spray development perspective, pyrolytic oil can be used as a substitute for conventional fuels in oil burners. Full article

Background/Objectives: All the industry standard methods for monitoring the freeze-drying process, from the single-vial assessment using temperature probes, such as thermocouples, to batch assessments using comparative pressure measurements, have poorly defined transitions marking the end of ice sublimation. In this study, through-vial impedance spectroscopy (TVIS) is used to characterise and validate the point on the Pirani curve that corresponds to the end of ice sublimation. The impact of the solution composition in relation to its propensity to form crystalline and amorphous domains and the impact of the batch size were investigated. Methods: Individual TVIS vials were placed at specific positions across the shelf, in order to represent the core and edge vials of the batch. The unique features of the high-frequency real part capacitance, with its precise sublimation endpoint-defining plateau, were then used to map the individual-vial sublimation endpoints onto the Pirani profile, with a view to predicting the batch sublimation endpoint. Results: TVIS vial endpoints enabled a key observation that the shape of the Pirani profile may be analysed in terms of two phases, the first being largely associated with ice sublimation and the second being associated with water desorption. Moreover, by identifying the transition point more precisely, even in the small to intermediate scale systems, we provide a scientific basis for predicting the sublimation endpoint for production-scale dryers, where Pirani sensors are already in place. Conclusions: Such qualification of batch sublimation endpoints would allow for earlier, confident switching to the secondary drying stage without unnecessary delay, leading to shorter cycles, reduced energy consumption, and improved utilisation of costly freeze-drying infrastructure. Full article

The collection and annotation of large-scale image datasets remains a significant challenge in training vision-based AI models, especially in domains such as industrial automation. In industrial settings, this limitation is especially critical for quality inspection tasks within Flexible Manufacturing Systems and Batch-Size-of-One production, where high variability in components restricts the availability of relevant datasets. This study presents a pipeline for generating photorealistic synthetic images to support automated visual inspection. Rendered images derived from geometric models of manufactured parts are enhanced using a Cycle-Consistent Adversarial Network (CycleGAN), which transfers pixel-level features from real camera images. The pipeline is applied in two scenarios: (1) domain transfer between similar objects for data augmentation, and (2) domain transfer between dissimilar objects to synthesize images before physical production. The generated images are evaluated using mean Average Precision (mAP) and the Turing test, respectively. The pipeline is further validated in two industrial setups: object detection for a pick-and-place task using a Niryo robot, and anomaly detection in products manufactured by a FESTO machine. The successful implementation of the pipeline demonstrates its potential to generate effective training data for vision-based AI in industrial applications and highlights the importance of enhancing domain quality in industrial synthetic data workflows. Full article

Xenodens calminechari is a highly derived mosasaurid from the latest Maastrichtian Phosphates of the Oulad Abdoun Basin, Morocco. Originally described based on a single maxilla, Xenodens differs from all known squamates in its closely packed, bladelike marginal teeth and modified tooth implantation and replacement. Xenodens’ relationships and anatomy remain poorly understood, and a recent study suggested that the holotype represents a composite, and furthermore that the animal might represent a juvenile of Carinodens. Evidence from a new referred specimen of Xenodens and CT scans corroborate the original description of Xenodens. Scans of the holotype and referred specimen of Xenodens reveal highly derived tooth implantation; interdental ridges are reduced in the posterior part of the jaw and teeth implant in a groove, with adjacent roots contacting and fusing. Tooth roots bear large, deep replacement pits, as is typical of derived mosasaurids, but in posterior teeth the replacement pits merge lingually to create a single large pit for two teeth. We provide an updated diagnosis of Xenodens, detailing unusual features of its tooth anatomy, implantation and replacement. Differences between Xenodens and Carinodens are numerous and no intermediate morphologies exist; furthermore, the size overlap between Carinodens and Xenodens indicates that Xenodens cannot represent a juvenile Carinodens. Xenodens highlights the remarkable diversity of mosasaurids, as well as the exceptional range of ecological niches occupied by this highly successful group of marine reptiles before their extinction. Full article

One of most important parts of the female genital tract is the uterine cervix, both from the anatomical as well as physiological points of view. As there is currently a lack of detailed information on the presence, distribution pattern(s), and the chemical coding of phoenixin (PNX)-containing dorsal root ganglia (DRG) neurons supplying the porcine uterine cervix, this study, using combined retrograde tracing and double-immunofluorescence techniques, was aimed at analyzing (i) the distribution pattern of uterine cervix-supplying sensory neurons (UC-SNs) at the particular spinal cord levels, (ii) their intraganglionic distribution, and (iii) the patterns of PNX co-expression with other biologically active substances. UC-SNs were identified by the presence of deposits of Fast Blue (FB), in DRG of thoracic (Th10–Th15), lumbar (L1–L5) and sacral (S2–S4) spinal cord segments. FB⁺/PNX⁺ neurons constitute approximately 33% of all UC-SNs, 73% at the L, and 27% at the S neuromeres. These neurons were mainly small sized (52%), with a slightly smaller population of medium-sized cells (40%), while large-diameter cells made up the least numerous population (8%). The vast majority of FB⁺/PNX⁺ neurons simultaneously contained calcitonin gene-related peptide (CGRP; 80.9%) or substance P (SP; 77.9%); one-third of them showed immunoreactivity toward neuronal nitric oxide synthase (nNOS; 34%), while PNX⁺ UC-SNs containing pituitary adenylate cyclase-activating polypeptide (PACAP), galanin (GAL), calretinin (CRT), or somatostatin (SOM) formed significantly smaller populations (21.4%, 7.4%, 3.1%, and 0.7%, respectively). The results of the present study demonstrate the presence of PNX in DRG UC-SNs, and its co-occurrence with numerous neurotransmitters suggesting a putative role for this neuropeptide in the transmission of various types of sensory information and possible effects on the functioning of this organ in the body. Full article