Search Results (66)

Host genetic variability is relevant to understanding how parasites modulate natural selection in wild fish populations. Coastal lagoons are transitional ecosystems where knowledge lacks on relationships between genotypic diversity with parasitism. The aim of this study was to assess the effect of genetic diversity on host health and parasitological traits in fish inhabiting a Mediterranean lagoon. Black-striped pipefish Syngnathus abaster were collected in August 2023 and 2024 from the Mar Menor (Iberian lagoon, SE Spain). Genetic diversity was measured as Internal Relatedness (IR: a homozygosity index from microsatellite markers). Population frequency was lower for the medium IR level. For this same category, both health indices (external body condition and internal organs) indicated a worse status. Parasite prevalence, abundance and an index of life-cycle complexity (heteroxenous species) were greater for the medium level of genetic diversity. Such results are explained under a scenario of parasite-mediated disruptive selection: a higher disease pressure against the phenotypically intermediate individuals. Two contrasting strategies were detected to better control parasitism at the host genotypic level: (1) high homozygosity, and (2) high heterozygosity, which probably reflects better immuno-competence as a phenotypic trait. From an evolutionary perspective, parasites play a crucial role in shaping genetic diversity within host populations. Full article

Sexual dimorphism is prevalent among animals, influencing both functional morphological traits and behavioral performances. In this study, we investigated the sexual differences in the morphological parameters of Kaloula borealis (Anura, Microhylidae) during the breeding season using 48 specimens. Our results reveal that among the 16 external morphological traits, females had significantly larger snout-vent length and eye diameter than males. The former presumably contributes to enhancing fertility, while the latter is associated with larger body size. Males exhibited significantly greater head width and thigh width than females, which may be related to accessing a wider range of food sources and enhancing their locomotor ability, respectively. Among the 32 appendicular muscles, 10 displayed significant sexual dimorphism in dry mass, suggesting divergent reproductive strategies between the sexes. Among the eight internal organs analyzed, males possessed significantly heavier hearts and lungs than females, which is likely an adaptation to higher metabolic demands and calling behavior. Collectively, our findings demonstrate that sex-specific differences in external morphology, muscle mass, and internal organ mass reflect distinct ecological and reproductive adaptations between males and females and contribute to the phenotypic diversities in Anura. Full article

Current tomato harvesters rely primarily on external color as the sole indicator of ripeness. However, this approach often results in premature harvesting, leading to insufficient lycopene accumulation and a suboptimal nutritional content for human consumption. Such limitations are especially critical in controlled-environment agriculture (CEA) systems, where maximizing fruit quality and nutrient density is essential for both the yield and consumer health. To address that challenge, this study introduces a novel, multimodal harvest readiness framework tailored to nutrient film technology (NFT)-based smart farms. The proposed approach integrates plant-level stress diagnostics and fruit-level phenotyping using wearable biosensors, AI-assisted computer vision, and non-invasive physiological sensing. Key physiological markers—including the volatile organic compound (VOC) methanol, phytohormones salicylic acid (SA) and indole-3-acetic acid (IAA), and nutrients nitrate and ammonium concentrations—are combined with phenomic traits such as fruit color (a*), size, chlorophyll index (rGb), and water status. The innovation lies in a four-stage decision-making pipeline that filters physiologically stressed plants before selecting ripened fruits based on internal and external quality indicators. Experimental validation across four plant conditions (control, water-stressed, light-stressed, and wounded) demonstrated the efficacy of VOC and hormone sensors in identifying optimal harvest candidates. Additionally, the integration of low-cost electrochemical ion sensors provides scalable nutrient monitoring within NFT systems. This research delivers a robust, sensor-driven framework for autonomous, data-informed harvesting decisions in smart indoor agriculture. By fusing real-time physiological feedback with AI-enhanced phenotyping, the system advances precision harvest timing, improves fruit nutritional quality, and sets the foundation for resilient, feedback-controlled farming platforms suited to meeting global food security and sustainability demands. Full article

Background/Objectives: This systematic review evaluates unconventional mammalian models from wild, agricultural, and urban/domestic ecosystems for genotoxicity assessment under the One Health framework. Non-human primates (NHPs), cattle, and domestic dogs are analyzed as sentinel species due to their distinct environmental niches, unique human interactions, and species-specific traits. In conjunction with this, evidence is presented about the in vitro use of cells of these mammals for the genotoxicological evaluation of different chemical substances, such as veterinary drugs, environmental pollutants, and pesticides. The synthesis focuses on standardized genetic toxicology assays (e.g., chromosomal aberrations, micronucleus, comet assay) aligned with Organization for Economic Cooperation and Development (OECD) guidelines. Methods: A structured search of international literature identified studies employing OECD-compliant genotoxicity assays in NHPs, cattle, dogs, and others not listed in OECD. Data was categorized by species, assay type, chemical class evaluated, environmental context (wild, agricultural, urban), and merits of the papers. Results: NHPs, despite their phylogenetic proximity to humans, show limited genotoxicity data in contrast to biomedical research, which has been constrained by ethical concerns and fieldwork logistics. Cattle emerge as robust models in agricultural settings due to the abundance of studies on the genotoxic capacity of pesticides, veterinary drug, and environmental biomonitoring, with direct implications for food safety. Domestic dogs are recognized as powerful sentinels for human health due to shared exposomes, physiological similarities (e.g., shorter cancer latency), and reduced lifestyle confounders; however, genotoxicity studies in dogs remain sparse compared to chemical exposure monitoring or cancer research. Conclusions: This review advocates for expanded, integrated use of these models to address genotoxic threats across ecosystems, which would benefit both animal and human health. In the application of biomonitoring studies with sentinel animals, a critical gap persists: the frequent lack of integration between xenobiotic quantification in environmental and biological samples, along with genotoxicity biomarkers evaluation in sentinel populations, which hinders comprehensive environmental risk assessment. Full article

Pear fruit quality is a key determinant of consumer preference, yet it remains insufficiently characterized in many newly developed cultivars. This study aimed to evaluate 25 pear genotypes (Pyrus communis L.), internationally renowned cultivars and new cultivars developed through Romanian breeding programs, with distinct ripening periods, using an integrative approach based on morphological, biochemical, and sensory traits. Standardized methods were applied to assess attributes including fruit size, firmness, soluble solids, organic acid composition, skin color, and hedonic sensory responses for taste, aroma, texture, and visual appeal. Results revealed significant variability across ripening groups, with several cultivars, such as ‘Paradox’, ‘Pandora’, ‘Isadora’, and ‘Daciana’, displaying favorable combinations of appearance, internal quality, and consumer-rated acceptability. ‘Paradox’ and ‘Pandora’ achieved the highest sensory scores, comparable to or surpassing those of commercial standards like ‘Packham’s Triumph’. Multivariate analyses confirmed trait correlations and distinct phenotypic profiles among cultivars. These findings underscore the utility of multidimensional quality assessment for selecting cultivars suited to modern market demands, both for fresh consumption and processing. Moreover, several genotypes demonstrated potential as parental sources in pear breeding programs targeting improved organoleptic and physicochemical traits. The study suggests that a differentiated sensory scoring approach, rather than a uniform 1–9 hedonic scale, may more effectively identify high-quality pear cultivars for breeding programs. Full article

Processed brown sugar is produced by combining non-centrifugal cane sugar (NCS), raw sugar, and molasses. The present study aimed to examine the effects of NCS and raw sugar blending (10%:90%, 50%:50%, 75%:25%, and 90%:10%) on color traits, non-volatile and volatile compounds, retronasal aroma release, and sensory profiles of processed brown sugar, and hence, its flavor quality. The International Commission for Uniform Methods of Sugar Analysis (ICUMSA) color index and the +L* (brightness) and +b* (yellowness) color spaces were gradually altered upon the addition of raw sugar, with strong Pearson’s negative correlations between the ICUMSA value and both color space indices (r = −0.9554 and r = −0.9739, respectively), causing a lighter color of the final product. Raw sugar addition also significantly reduced the concentration of non-volatile compounds, such as glucose and organic acids (p < 0.05). As the raw sugar proportion increased from 10 to 90%, the concentrations of total volatile compounds and Maillard reaction products (MRPs), such as pyrazines, furans, and furanones, also decreased significantly from 62.58 to 22.73 µg/100 g and 34.75 to 6.80 µg/100 g, respectively. Reduced intensities of ion masses of in-mouth and in-nose retronasal odors from volatile MRPs, as well as roasted aroma and richness properties, were observed in processed brown sugars with greater raw sugar content. Taken together, a higher proportion of raw sugar in processed brown sugar manufacturing enhances brightness while reducing acidity and aftertaste; however, increased NCS content results in darker products with greater roasted aroma and richness, affecting flavor quality. Full article

The Chinese soft-shelled turtle (Pelodiscus sinensis) is an aquatic reptile prized for its nutritional and health benefits. Given its adaptability to various culturing modes including the greenhouse, pond and rice culturing modes, we conducted a comparative analysis of the morphology, organ trait and nutritional composition of turtles cultured in three culturing modes. This study investigated the plasticity of morphology and physiology, as well as the variations in nutritional composition across varying culturing modes. The results demonstrated that after approximately 120 days of cultivation, significant changes were observed in the morphology, physiology and nutritional composition of turtles from each culturing mode. In terms of morphology, rice turtles exhibited an arched shell shape, broad plastron, elongated limbs, narrow interocular distance and slender head and neck. Pond turtles displayed similar morphological characteristics to rice turtles, with the additional features of a flattened body shape and narrower plastron. Greenhouse turtles presented a flattened shell shape, narrow plastron, shortened limbs, wider interocular distance and stocky head and neck. Regarding the organ characteristics, the specific weights of liver, viscera, internal fat lumps and condition factors were significantly higher in greenhouse turtles compared to rice turtles and pond turtles (p < 0.05). Conversely, the specific weights of the back carapace, calipash and edible part were significantly lower than those in rice turtles and pond turtles (p < 0.05). Nutritional analysis revealed that crude protein, total amino acid, essential amino acid, flavor amino acid, pharmacodynamic amino acid, collagen and EPA+DHA contents were significantly higher in rice turtles and pond turtles than greenhouse turtles (p < 0.05). However, crude fat and unsaturated fatty acid contents were significantly higher in greenhouse turtles than in rice turtles and pond turtles (p < 0.05). In summary, Chinese soft-shelled turtles exhibited significant morphological and organ plasticity in response to different culturing modes. While the rice and pond culturing modes could enhance the nutritional quality of turtles to some extent, the impact of commercial feed on fatty acid profiles must be carefully considered. Full article

Flowers, serving as the reproductive structures of angiosperms, perform an integral role in plant biology and are fundamental to understanding plant evolution and taxonomy. The growth and organogenesis of flowers are driven by numerous factors, such as external environmental conditions and internal physiological processes, resulting in diverse traits across species or even within the same species. Among these factors, genes play a central role, governing the entire developmental process. The regulation of floral genesis by these genes has become a significant focus of research. In the AE model of floral development, the five structural whorls (calyx, corolla, stamens, pistils, and ovules) are controlled by five groups of genes: A, B, C, D, and E. These genes interact to give rise to a complex control system that governs the floral organsgenesis. The activation or suppression of specific gene categories results in structural modifications to floral organs, with variations observed across different species. The present article examines the regulatory roles of key genes, including genes within the MADS-box and AP2/ERF gene clusters, such as AP1, AP2, AP3, AG, STK, SHP, SEP, PI, and AGL6, as well as other genes, like NAP, SPL, TGA, PAN, and WOX, in shaping floral organ genesis. In addition, it analyzes the molecular-level effects of these genes on floral organ formation. The findings offer a deeper understanding of the genetic governance of floral organ genesis across plant species. Full article

Artificial rearing (AR) of lambs is nowadays a common practice in Mediterranean dairy sheep production systems to enhance the milk available for cheese or yoghurt manufacturing. The sufficient growth of lambs in an AR system is vital for the economic success of dairy sheep farms. However, AR is often associated with negative impacts on the performance and physiology of lambs. Greece is one of the major producers of ovine milk; nevertheless, data concerning the effects of artificial rearing in lambs of Greek autochthonous breeds are not available. Therefore, the present study aimed to examine the influence of artificial rearing on growth performance, thyroid hormone levels, locomotor activity, carcass traits and meat quality characteristics in lambs of the Chios breed, which is one of the most well-known Greek dairy sheep breeds. Twenty-one singleton male lambs were assigned into two feeding regimes; natural rearing NR (n = 11) and AR (n = 10). The lambs’ behavior was continuously videotaped until weaning, and their standing percentage was recorded as an activity index. Blood samples were collected from the jugular vein of lambs on days 3, 10, 17 and 40 after birth to assess thyroid hormone levels. The body weight of lambs was also recorded weekly. At the age of 45 days, lambs were fasted for 12 h, weighed and slaughtered. The weights of the carcass and internal organs were measured, while samples of the longissimus dorsi muscle were used for the determination of meat pH, color, water holding capacity, shear force and oxidative stability values. As indicated, body weight (kg) at birth was greater in NR vs. AR group and this difference was maintained till day 35 (p < 0.05), although body gain (kg) was generally not significantly different between NR and AR lambs, with the exception of the first week, when NR showed a greater value compared with the AR lambs (p < 0.001). On day 42, no significant differences between lamb groups for body weight were observed. Levels for triiodothyronine (T3), thyroxine (T4) and the free form of T3 (FT3) were greater, whereas the standing percentage was lower in NR compared with AR lambs (p < 0.05). The feeding regime of lambs did not affect carcass traits, internal organ and fat tissue weights, except for cold carcass yield which was greater in AR vs. NR lambs. No significant differences were observed between the two lamb groups in meat quality characteristics, such as pH, color, water holding capacity and shear force values, although MDA content was decreased in AR lambs indicating an improved oxidative stability. In conclusion, artificial rearing appears to be a feasible strategy for Chios lamb meat production, since it does not negatively influence carcass traits and meat quality characteristics, while a positive effect in meat oxidative stability is observed. Full article

Fungi are one of the major components of the eukaryotic microbial community in marine ecosystems, playing a significant role in organic matter cycling and food web dynamics. However, the diversity and roles of fungi in marine sediments remain poorly documented. To elucidate the diversity and spatial distribution of fungal communities in the marine sediments of an estuary–coast continuum across three distinct salinity regions in Zhanjiang Bay, China, the variations in fungal diversity, abundance, community structure, and distribution in the sediments were investigated through the application of high-throughput amplicon sequencing using the internal transcribed spacer (ITS) primers. Additionally, the FUNGuild database was employed to assess the potential functional traits of fungi. A total of 1242 ASV sequences, affiliated to 144 genera and five phyla, were identified. Ascomycota (68.97%) and Basidiomycota (6.41%) were the dominant fungal groups, together accounting for 75.38% of the total relative abundance of the fungal community. Significant differences were observed in the α-diversity indices (Shannon index and richness) and β-diversity of fungal communities across the three distinct salinity regions. The fungal molecular network exhibited primarily positive species interactions, with notable structural differences across salinity gradients. The low-salinity group had a large network with high modularity; the medium-salinity group a small, simple network with high centralization, and the high-salinity group a compact, moderately complex network. Symbiotrophs, saprotrophs, and pathotrophs, being the three trophic types with the highest proportions, were estimated based on ITS. A redundancy analysis (RDA) indicated that salinity was the primary factor influencing the distribution of Ascomycota communities, while the distributions of Basidiomycota, Chytridiomycota, Mucoromycota, and Rozellomycota were more strongly affected by environmental factors such as chlorophyll a, chemical oxygen demand (COD), pH, and temperature. Our work provides new scientific data on the diversity, composition, and distribution of fungal communities in Zhanjiang Bay, which helps to understand the biodiversity of fungi in the estuary–coast ecosystems. Full article

Remontant raspberry cultivars originally produce fruit in the upper part of primocanes in the fall, but if retained over winter, they can produce a second crop in the lower part of the floricanes the following spring. Maintaining remontant cultivars to yield twice during the cane’s growth cycle corresponds to a double-cropping system, which enables an increase in the total yield and the extension of the fruiting season. To date, there is little information on changes in fruit quality between primocane and floricane crops. The aim of this study was, therefore, to investigate variations in the content of sugars and organic acids, fruit weight, color and textural and sensorial attributes among five newly introduced remontant raspberry cultivars (‘Dafne’, ‘Kokanee’, ‘Paris’, ‘Versailles’ and ‘Primalba’) and the control cultivar ‘Enrosadira’. The specific aim was to evaluate how a double-cropping system in each cultivar may affect the variability in quality traits between primocane and floricane crops. The results showed a significant increase in fruit weight and individual and total sugar content in primocane crops, while significantly brighter red-colored and firmer fruits were observed in floricane crops. Cultivars did not differ from the control regarding total sugar content and sweetness index, while the content of individual sugars caused greater variations. The highest content of citric, malic and total acid (9.74, 1.42 and 11.25 mg 100 g⁻¹ FW, respectively) were recorded in ‘Paris’, by which this cultivar was the only one distinguished from the control. ‘Dafne’ and ‘Versailles’ exhibited better internal and external fruit quality on primocanes, having significantly larger fruits (6.83 g and 6.37 g, respectively) and twice the increased sugar content. The lowest fruit weight was observed in ‘Kokanee’ for both primocane (4.63 g) and floricane (3.65 g) crops. ‘Kokanee’ and ‘Primalba’ also performed worse than the control for most sensory attributes in both seasons. Based on the analysis of the overall fruit quality linked to the appearance-, texture- and taste-related attributes that affect consumer preference, cultivars ‘Enrosadira’, ‘Versailles’ and ‘Dafne’ stood out, while ‘Paris’ showed high uniformity in fruit quality between crops, but scored the worst according to the total quality index. Full article

Automatic acquisition of phenotypic traits in tomato plants is important for tomato variety selection and scientific cultivation. Because of time-consuming and labor-intensive traditional manual measurements, the lack of complete structural information in two-dimensional (2D) images, and the complex structure of the plants, it is difficult to automatically obtain the phenotypic traits of the tomato canopy. Thus, a method for calculating the phenotypic traits of tomato canopy in greenhouse was proposed based on the extraction of the branch skeleton. First, a top-view-based acquisition platform was built to obtain the point cloud data of the tomato canopy, and the improved K-means algorithm was used to segment the three-dimensional (3D) point cloud of branches. Second, the Laplace algorithm was used to extract the canopy branch skeleton structure. Branch and leaf point cloud separation was performed using branch local skeleton vectors and internal features. In addition, the DBSCAN clustering algorithm was applied to recognize individual leaf organs. Finally, phenotypic traits including mean leaf inclination, digital biomass, and light penetration depth of tomato canopies were calculated separately based on the morphological structure of the 3D point cloud. The experimental results show that the detection accuracies of branches and leaves were above 88% and 93%, respectively, and the coefficients of determination between the calculated and measured values of mean leaf inclination, digital biomass, and light penetration depth were 0.9419, 0.9612, and 0.9093, respectively. The research results can provide an effective quantitative basis and technical support for variety selection and scientific cultivation of the tomato plant. Full article

Plant architecture is an important agronomic trait that impacts crop yield. The tiller angle is a critical aspect of the plant’s structural organization, which is influenced by both internal and external factors. The genetic mechanisms underlying the tiller angle have been extensively investigated in other plants. However, research on wheat is relatively limited. Additionally, mechanics has emerged as a connection between biochemical signaling and the development of three-dimensional biological forms. It not only reveals how physical interactions at the cellular level influence overall morphogenesis but also elucidates the interplay between these mechanical processes and molecular signaling pathways that collectively determine plant morphology. This review examines the recent advancements in the study of tillering angle in wheat and other plants. It discusses progress in research ranging from observable characteristics to the regulation of genes, as well as the physiological and biochemical aspects, and the adaptability to environmental factors. In addition, this review also discusses the effects of mechanical on plant growth and development, and provides ideas for the study of mechanical regulation mechanism of tillering angle in wheat. Consequently, based on the research of other plants and combined with the genetic and mechanical principles, this approach offers novel insights and methodologies for studying tillering in wheat. This interdisciplinary research framework not only enhances our understanding of the mechanisms underlying wheat growth and development but may also uncover the critical factors that regulate tillering angle, thereby providing a scientific foundation for improving wheat yield and adaptability. Full article

Cnidarians are among the most important diploblastic organisms, elucidating many of the early stages of Metazoan evolution. However, Cnidarian fossils from Cambrian deposits have been rarely documented, mainly due to difficulties in identifying early Cnidarian representatives. Halysites, a tabulate coral from Silurian reef systems, serves as a crucial taxon for interpreting Cambrian cnidarians. Traditionally, the biological characteristics of Halysites have been analyzed using methods limited by pretreatment requirements (destructive testing) and the chamber size capacity of relevant analytical instruments. These constraints often lead to irreversible information loss and inadequate data extraction. This means that, to date, there has been no high-resolution three-dimensional mineralization analysis of Halysites. This study aims to introduce novel, non-destructive techniques to analyze the internal structure and chemical composition of Halysites. Furthermore, it seeks to elucidate the relationship between coral organisms and biomineralization in reef settings and to compare Silurian Tabulata with putative Cambrian cnidarians. Techniques such as micro-X-ray fluorescence spectrometry (micro-XRF), micro-X-ray computed tomography (micro-CT), and scanning electron microscopy (SEM) were employed in this research. With the help of high-resolution micro-CT scanning, we identify the growth pattern of Halysites, showing both lateral and vertical development. The lateral multiple-branching growth pattern of Halysites corals is first established herein. The flaggy corallite at the initial stage of branching is also observed. The micro-XRF mapping results reveal the occurrence of septa spines for Halysites, a trait previously thought rare or absent. Additionally, the ratio of coral volume to the surrounding rock was assessed, revealing that Halysites reefs were relatively sparse (volume ratio = ~30%). The cavities between Halysites likely provided more space for other organisms (e.g., rugose corals and bryozoans) when compared to other coral reef types. Additionally, we provide a comparative analysis of post-Cambrian colonial calcareous skeletons, offering insights into the structural features and growth patterns of early skeletal metazoans across the Ediacaran–Cambrian boundary. Full article

Arrhythmias and depression are recognized as diseases of the heart and brain, respectively, and both are major health threats that often co-occur with a bidirectional causal relationship. The autonomic nervous system (ANS) serves as a crucial component of the heart–brain axis (HBA) and the pathway of interoception. Cardiac activity can influence emotional states through ascending interoceptive pathways, while psychological stress can precipitate arrhythmias via the ANS. However, the HBA and interoception frameworks are often considered overly broad, and the precise mechanisms underlying the bidirectional relationship between depression and arrhythmias remain unclear. This narrative review aims to synthesize the existing literature, focusing on the pathological mechanisms of the ANS in depression and arrhythmia while integrating other potential mechanisms to detail heart–brain interactions. In the bidirectional communication between the heart and brain, we emphasize considering various internal factors such as genes, personality traits, stress, the endocrine system, inflammation, 5-hydroxytryptamine, and behavioral factors. Current research employs multidisciplinary knowledge to elucidate heart–brain relationships, and a deeper understanding of these interactions can help optimize clinical treatment strategies. From a broader perspective, this study emphasizes the importance of considering the body as a complex, interconnected system rather than treating organs in isolation. Investigating heart–brain interactions enhance our understanding of disease pathogenesis and advances medical science, ultimately improving human quality of life. Full article