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Keywords = hyperspectral control

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18 pages, 2475 KB  
Article
A Machine Learning Framework for Classifying Thermal Stress in Bean Plants Using Hyperspectral Data
by Lucas Prado Osco, Érika Akemi Saito Moriya, Bruna Coelho de Lima, Ana Paula Marques Ramos, José Marcato Júnior, Wesley Nunes Gonçalves, Lúcio André de Castro Jorge, Veraldo Liesenberg, Jonathan Li, Ademir Sérgio Ferreira de Araújo, Nilton Nobuhiro Imai and Fábio Fernando de Araújo
AgriEngineering 2025, 7(11), 376; https://doi.org/10.3390/agriengineering7110376 - 7 Nov 2025
Viewed by 199
Abstract
Rising global temperatures pose a significant threat to agricultural productivity, making the early detection of plant stress crucial for minimizing crop losses. While hyperspectral remote sensing is a powerful tool for monitoring plant health, the precise spectral regions and most effective machine learning [...] Read more.
Rising global temperatures pose a significant threat to agricultural productivity, making the early detection of plant stress crucial for minimizing crop losses. While hyperspectral remote sensing is a powerful tool for monitoring plant health, the precise spectral regions and most effective machine learning models for detecting thermal stress remain an open research question. This study presents a robust framework that utilizes eight state-of-the-art machine learning algorithms to classify the reflectance response of thermal-induced stress in two cultivars of bean plants. Our controlled experiment measured hyperspectral data across two growth stages and three stress conditions (pre-stress, during stress, and post-stress) using a spectroradiometer. The results demonstrate the high performance of several algorithms, with the Artificial Neural Network (ANN) achieving an impressive 99.4% overall accuracy. A key contribution of this work is the identification of the most contributory spectral ranges for thermal stress discrimination: the green region (530–570 nm) and the red-edge region (700–710 nm). This framework is a feasible and effective tool for modelling the hyperspectral response of thermal-stressed bean plants and provides critical guidance for future research on stress-specific spectral indices. Full article
(This article belongs to the Special Issue Remote Sensing for Enhanced Agricultural Crop Management)
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37 pages, 3061 KB  
Article
Deep Learning-Based Digital, Hyperspectral, and Near-Infrared (NIR) Imaging for Process-Level Quality Control in Ecuador’s Agri-Food Industry: An ISO-Aligned Framework
by Alexander Sánchez-Rodríguez, Richard Dennis Ullrich-Estrella, Carlos Ernesto González-Gallardo, María Belén Jácome-Villacres, Gelmar García-Vidal and Reyner Pérez-Campdesuñer
Processes 2025, 13(11), 3544; https://doi.org/10.3390/pr13113544 - 4 Nov 2025
Viewed by 297
Abstract
Ensuring consistent quality and safety in agri-food processing is a strategic priority for firms seeking compliance with international standards such as ISO 9001 and ISO 22000. Traditional inspection practices in Ecuador’s food industry remain largely destructive, labor-intensive, and subjective, limiting real-time decision-making. This [...] Read more.
Ensuring consistent quality and safety in agri-food processing is a strategic priority for firms seeking compliance with international standards such as ISO 9001 and ISO 22000. Traditional inspection practices in Ecuador’s food industry remain largely destructive, labor-intensive, and subjective, limiting real-time decision-making. This study developed a non-destructive, ISO-aligned framework for process-level quality control by integrating digital (RGB) imaging for surface-level inspection, hyperspectral imaging (HSI) for internal-quality prediction (e.g., moisture, firmness, and freshness), near-infrared spectroscopy (NIRS) for compositional and authenticity analysis, and deep learning (DL) models for automated classification of ripeness, maturity, and defects. Experimental results across four flagship commodities—bananas, cacao, coffee, and shrimp—achieved classification accuracies above 88% and ROC AUC values exceeding 0.90, confirming the robustness of AI-driven, multimodal (RGB–HSI–NIRS) inspection under semi-industrial conveyor conditions. Beyond technological performance, the findings demonstrate that digital inspection reinforces ISO principles of evidence-based decision-making, conformity verification, and traceability, thereby operationalizing the Plan–Do–Check–Act (PDCA) cycle at digital speed. The study contributes theoretically by advancing the conceptualization of Quality 4.0 as a socio-technical transformation that embeds AI-driven sensing and analytics within management standards, and practically by providing a roadmap for Ecuadorian SMEs to strengthen export competitiveness through automated, real-time, and auditable quality assurance. Full article
(This article belongs to the Special Issue Processing and Quality Control of Agro-Food Products)
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28 pages, 9838 KB  
Article
Evaluating the Performance of Hyperspectral Imaging Endoscopes: Mitigating Parameters Affecting Spectral Accuracy
by Siavash Mazdeyasna, Mohammed Shahriar Arefin, Andrew Fales, Silas J. Leavesley, T. Joshua Pfefer and Quanzeng Wang
Biosensors 2025, 15(11), 738; https://doi.org/10.3390/bios15110738 - 4 Nov 2025
Viewed by 364
Abstract
Hyperspectral imaging (HSI) is increasingly used in studies for medical applications as it provides both structural and functional information of biological tissue, enhancing diagnostic accuracy and clinical decision-making. Recently, HSI cameras (HSICs) have been integrated with medical endoscopes (HSIEs), capturing hypercube data beyond [...] Read more.
Hyperspectral imaging (HSI) is increasingly used in studies for medical applications as it provides both structural and functional information of biological tissue, enhancing diagnostic accuracy and clinical decision-making. Recently, HSI cameras (HSICs) have been integrated with medical endoscopes (HSIEs), capturing hypercube data beyond conventional white light imaging endoscopes. However, there are currently no cleared or approved HSIEs by the U.S. Food and Drug Administration (FDA). HSI accuracy depends on technologies and experimental parameters, which must be assessed for reliability. Importantly, the reflectance spectrum of a target can vary across different cameras and under different environmental or operational conditions. Thus, before reliable clinical translation can be achieved, a fundamental question must be addressed: can the same target yield consistent spectral measurements across different HSI systems and under varying acquisition conditions? This study investigates the impact of eight parameters—ambient light, exposure time, camera warm-up time, spatial and temporal averaging, camera focus, working distance, illumination angle, and target angle—on spectral measurements using two HSI techniques: interferometer-based spectral scanning and snapshot. Controlled experiments were conducted to evaluate how each parameter affects spectral accuracy and whether normalization can mitigate these effects. Our findings reveal that several parameters significantly influence spectral measurements, with some having a more pronounced impact. While normalization reduced variations for most parameters, it was less effective at mitigating errors caused by ambient light and camera warm-up time. Additionally, normalization did not eliminate spectral noise resulting from low exposure time, small region of interest, or a spectrally non-uniform light source. From these results, we propose practical considerations for optimizing HSI system performance. Implementing these measures can minimize variations in reflectance spectra of identical targets captured by different cameras and under diverse conditions, thereby supporting the reliable translation of HSI techniques to clinical applications. Full article
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20 pages, 3577 KB  
Article
Hyperspectral Remote Sensing and Artificial Intelligence for High-Resolution Soil Moisture Prediction
by Ki-Sung Kim, Junwon Lee, Jeongjun Park, Gigwon Hong and Kicheol Lee
Water 2025, 17(21), 3069; https://doi.org/10.3390/w17213069 - 27 Oct 2025
Viewed by 503
Abstract
Reliable field estimation of soil moisture supports hydrology and water resources management. This study develops a drone-based hyperspectral approach in which visible and near-infrared reflectance is paired one-to-one with gravimetric water content measured by oven drying, yielding 1000 matched samples. After standardization, outlier [...] Read more.
Reliable field estimation of soil moisture supports hydrology and water resources management. This study develops a drone-based hyperspectral approach in which visible and near-infrared reflectance is paired one-to-one with gravimetric water content measured by oven drying, yielding 1000 matched samples. After standardization, outlier control, ranked wavelength selection, and light feature engineering, several predictors were evaluated. Conventional machine learning methods, including simple and multiple regression and tree-based ensembles, were limited by band collinearity and piecewise approximations and therefore failed to meet the accuracy target. Gradient boosting reached the target but used different trade-offs in variable sensitivity. An artificial neural network with three hidden layers, rectified linear unit activations, and dropout was trained using a feature count sweep and early stopping. With ten predictors, the model achieved a coefficient of determination of 0.9557, demonstrating accurate mapping from hyperspectral reflectance to gravimetric water content and providing a reproducible framework suitable for larger, multi date acquisitions and operational decision support. Full article
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22 pages, 2704 KB  
Article
Cross-Crop Transferability of Machine Learning Models for Early Stem Rust Detection in Wheat and Barley Using Hyperspectral Imaging
by Anton Terentev, Daria Kuznetsova, Alexander Fedotov, Olga Baranova and Danila Eremenko
Plants 2025, 14(21), 3265; https://doi.org/10.3390/plants14213265 - 25 Oct 2025
Viewed by 388
Abstract
Early plant disease detection is crucial for sustainable crop production and food security. Stem rust, caused by Puccinia graminis f. sp. tritici, poses a major threat to wheat and barley. This study evaluates the feasibility of using hyperspectral imaging and machine learning [...] Read more.
Early plant disease detection is crucial for sustainable crop production and food security. Stem rust, caused by Puccinia graminis f. sp. tritici, poses a major threat to wheat and barley. This study evaluates the feasibility of using hyperspectral imaging and machine learning for early detection of stem rust and examines the cross-crop transferability of diagnostic models. Hyperspectral datasets of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) were collected under controlled conditions, before visible symptoms appeared. Multi-stage preprocessing, including spectral normalization and standardization, was applied to enhance data quality. Feature engineering focused on spectral curve morphology using first-order derivatives, categorical transformations, and extrema-based descriptors. Models based on Support Vector Machines, Logistic Regression, and Light Gradient Boosting Machine were optimized through Bayesian search. The best-performing feature set achieved F1-scores up to 0.962 on wheat and 0.94 on barley. Cross-crop transferability was evaluated using zero-shot cross-domain validation. High model transferability was confirmed, with F1 > 0.94 and minimal false negatives (<2%), indicating the universality of spectral patterns of stem rust. Experiments were conducted under controlled laboratory conditions; therefore, direct field transferability may be limited. These findings demonstrate that hyperspectral imaging with robust preprocessing and feature engineering enables early diagnostics of rust diseases in cereal crops. Full article
(This article belongs to the Special Issue Application of Optical and Imaging Systems to Plants)
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19 pages, 1792 KB  
Article
Hyperspectral Detection of Single and Combined Effects of Simulated Tree Shading and Alternaria alternata Infection on Sorghum bicolor, from Leaf to UAV-Canopy Scale
by Lorenzo Pippi, Michael Alibani, Nicola Acito, Daniele Antichi, Giovanni Caruso, Marco Fontanelli, Michele Moretti, Cristina Nali, Silvia Pampana, Elisa Pellegrini, Andrea Peruzzi, Samuele Risoli, Gabriele Sileoni, Nicola Silvestri, Lorenzo Gabriele Tramacere and Lorenzo Cotrozzi
Agronomy 2025, 15(11), 2458; https://doi.org/10.3390/agronomy15112458 - 22 Oct 2025
Viewed by 362
Abstract
Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the [...] Read more.
Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the urgent need for advanced plant health monitoring in these systems. This study assessed the potential of hyperspectral reflectance to detect the single and combined effects of simulated tree shading and infection by the fungal pathogen Alternaria alternata on grain sorghum (Sorghum bicolor L. Moench) under rainfed field conditions. Sorghum was grown either under full light or 50% shading conditions. Half of the plots were artificially inoculated with an A. alternata spore suspension (2 × 108 CFU mL−1), while the others served as controls. Leaf and ground-canopy measurements were acquired with a full range spectroradiometer (VNIR-SWIR, 400–2,400 nm) and UAV imagery covered the VIS-NIR range (400–1,000 nm) before the onset of visible symptoms. Permutational multivariate analysis of variance of leaf and ground-canopy data revealed significant effects of shading (Sh), infection (Aa), and their interaction (p < 0.05), allowing early detection of infection two days before symptom appearance, while UAV data showed only singular significant effects. Partial least squares discriminant analysis accuracy reached 78% at the leaf level, 90% at the ground-canopy level, and 74% (Sh) and 75% (Aa) at the UAV scale. Furthermore, vegetation spectral indices derived from the spectra confirmed greater physiological stress in shaded and infected plants, consistent with disease incidence assessments. Our results establish scale-specific hyperspectral reflectance spectroscopy as a powerful, non-destructive technique for early plant health surveillance in agroforestry. This advanced optical sensing capability is poised to illuminate complex stressor interactions, marking a significant step forward for precision agroforestry management. Full article
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24 pages, 8373 KB  
Article
Sensitivity of Airborne Methane Retrieval Algorithms (MF, ACRWL1MF, and DOAS) to Surface Albedo and Types: Hyperspectral Simulation Assessment
by Jidai Chen, Ding Wang, Lizhou Huang and Jiasong Shi
Atmosphere 2025, 16(11), 1224; https://doi.org/10.3390/atmos16111224 - 22 Oct 2025
Viewed by 268
Abstract
Methane (CH4) emissions are a major contributor to greenhouse gases and pose significant challenges to global climate mitigation efforts. The accurate determination of CH4 concentrations via remote sensing is crucial for emission monitoring but remains impeded by surface spectral heterogeneity—notably [...] Read more.
Methane (CH4) emissions are a major contributor to greenhouse gases and pose significant challenges to global climate mitigation efforts. The accurate determination of CH4 concentrations via remote sensing is crucial for emission monitoring but remains impeded by surface spectral heterogeneity—notably albedo variations and land cover diversity. This study systematically assessed the sensitivity of three mainstream algorithms, namely, matched filter (MF), albedo-corrected reweighted-L1-matched filter (ACRWL1MF), and differential optical absorption spectroscopy (DOAS), to surface type, albedo, and emission rate through high-fidelity simulation experiments, and proposed a dynamic regularized adaptive matched filter (DRAMF) algorithm. The experiments simulated airborne hyperspectral imagery from the Airborne Visible/InfraRed Imaging Spectrometer-Next Generation (AVIRIS-NG) with known CH4 concentrations over diverse surfaces (including vegetation, soil, and water) and controlled variations in albedo through the large-eddy simulation (LES) mode of the Weather Research and Forecasting (WRF) model and the MODTRAN radiative transfer model. The results show the following: (1) MF and DOAS have higher true positive rates (TP > 90%) in high-reflectivity scenarios, but the problem of false positives is prominent (TN < 52%); ACRWL1MF significantly improves the true negative rate (TN = 95.9%) through albedo correction but lacks the ability to detect low concentrations of CH4 (TP = 63.8%). (2) All algorithms perform better at high emission rates (1000 kg/h) than at low emission rates (500 kg/h), but ACRWL1MF performs more robustly in low-albedo scenarios. (3) The proposed DRAMF algorithm improves the F1 score (0.129) by about 180% compared to the MF and DOAS algorithms and improves TP value (81.4%) by about 128% compared to the ACRWL1MF algorithm through dynamic background updates and an iterative reweighting mechanism. In practical applications, the DRAMF algorithm can also effectively monitor plumes. This research indicates that algorithms should be selected considering the specific application scenario and provides a direction for technical improvements (e.g., deep learning model) for monitoring gas emission. Full article
(This article belongs to the Special Issue Satellite Remote Sensing Applied in Atmosphere (3rd Edition))
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30 pages, 1303 KB  
Review
Spectral Reconstruction Applied in Precision Agriculture: On-Field Solutions
by Marco Mingrone, Marco Seracini and Chiara Cevoli
Appl. Sci. 2025, 15(20), 10985; https://doi.org/10.3390/app152010985 - 13 Oct 2025
Viewed by 586
Abstract
Over the past two decades, hyperspectral imaging (HSI) systems have shown significant potential in agriculture, from disease detection to the assessment of plant and fruit nutritional status. However, most applications remain confined to laboratory analyses under controlled conditions, with only a limited fraction [...] Read more.
Over the past two decades, hyperspectral imaging (HSI) systems have shown significant potential in agriculture, from disease detection to the assessment of plant and fruit nutritional status. However, most applications remain confined to laboratory analyses under controlled conditions, with only a limited fraction implemented in field environments. In this scenario, spectral reconstruction techniques may serve as a bridge between the high accuracy of HSI and the challenges of on-field or even real-time applications. This review outlines the current state of the art of on-field HSI in the agrifood sector, highlighting existing limitations and potential advantages. It then introduces the problem of spectral reconstruction and reviews current techniques used to address it. Laboratory and on-field studies will be taken into account. The final section offers our perspective on the limitations of HSI and the promising potential of spectral super-resolution to overcome current barriers and enable broader adoption of hyperspectral technology in precision agriculture. Full article
(This article belongs to the Special Issue Signal and Image Processing: From Theory to Applications: 2nd Edition)
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35 pages, 5316 KB  
Review
Machine Learning for Quality Control in the Food Industry: A Review
by Konstantinos G. Liakos, Vassilis Athanasiadis, Eleni Bozinou and Stavros I. Lalas
Foods 2025, 14(19), 3424; https://doi.org/10.3390/foods14193424 - 4 Oct 2025
Viewed by 3115
Abstract
The increasing complexity of modern food production demands advanced solutions for quality control (QC), safety monitoring, and process optimization. This review systematically explores recent advancements in machine learning (ML) for QC across six domains: Food Quality Applications; Defect Detection and Visual Inspection Systems; [...] Read more.
The increasing complexity of modern food production demands advanced solutions for quality control (QC), safety monitoring, and process optimization. This review systematically explores recent advancements in machine learning (ML) for QC across six domains: Food Quality Applications; Defect Detection and Visual Inspection Systems; Ingredient Optimization and Nutritional Assessment; Packaging—Sensors and Predictive QC; Supply Chain—Traceability and Transparency and Food Industry Efficiency; and Industry 4.0 Models. Following a PRISMA-based methodology, a structured search of the Scopus database using thematic Boolean keywords identified 124 peer-reviewed publications (2005–2025), from which 25 studies were selected based on predefined inclusion and exclusion criteria, methodological rigor, and innovation. Neural networks dominated the reviewed approaches, with ensemble learning as a secondary method, and supervised learning prevailing across tasks. Emerging trends include hyperspectral imaging, sensor fusion, explainable AI, and blockchain-enabled traceability. Limitations in current research include domain coverage biases, data scarcity, and underexplored unsupervised and hybrid methods. Real-world implementation challenges involve integration with legacy systems, regulatory compliance, scalability, and cost–benefit trade-offs. The novelty of this review lies in combining a transparent PRISMA approach, a six-domain thematic framework, and Industry 4.0/5.0 integration, providing cross-domain insights and a roadmap for robust, transparent, and adaptive QC systems in the food industry. Full article
(This article belongs to the Special Issue Artificial Intelligence for the Food Industry)
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21 pages, 5051 KB  
Article
Identification of Hybrid Indica Paddy Rice Grain Varieties Based on Hyperspectral Imaging and Deep Learning
by Meng Zhang, Peng Li, Wei Dong, Shuqi Tang, Yan Wang, Runmei Li, Shucun Ju, Bolun Guan, Jingbo Zhu, Juanjuan Kong and Liping Zhang
Biosensors 2025, 15(10), 647; https://doi.org/10.3390/bios15100647 - 30 Sep 2025
Viewed by 540
Abstract
Paddy rice grain variety classification is essential for quality control, as different rice varieties exhibit significant variations in quality attributes, affecting both food security and market value. The integration of hyperspectral imaging with machine learning presents a promising approach for precise classification, though [...] Read more.
Paddy rice grain variety classification is essential for quality control, as different rice varieties exhibit significant variations in quality attributes, affecting both food security and market value. The integration of hyperspectral imaging with machine learning presents a promising approach for precise classification, though challenges remain in managing the high dimensionality and variability of spectral data, along with the need for model interpretability. To address these challenges, this study employs a CNN-Transformer model that incorporates Standard Normal Variate (SNV) preprocessing, Competitive Adaptive Reweighted Sampling (CARS) for feature wavelength selection, and interpretability analysis to optimize the classification of hybrid indica paddy rice grain varieties. The results show that the CNN-Transformer model outperforms baseline models, achieving an accuracy of 95.33% and an F1-score of 95.40%. Interpretability analysis reveals that the model’s ability to learn from key wavelength features is significantly stronger than that of the comparison models. The key spectral bands identified for hybrid indica paddy rice grain variety classification lie within the 400–440 nm, 580–700 nm, and 880–960 nm ranges. This study demonstrates the potential of hyperspectral imaging combined with machine learning to advance rice variety classification, providing a powerful and interpretable tool for automated rice quality control in agricultural practices. Full article
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18 pages, 1569 KB  
Article
Data-Driven Optimization of Substrate Composition for Lettuce in Soilless Cultivation
by Ziran Ye, Lupin Deng, Mengdi Dai, Yu Luo, Dedong Kong and Xiangfeng Tan
Horticulturae 2025, 11(10), 1153; https://doi.org/10.3390/horticulturae11101153 - 25 Sep 2025
Viewed by 756
Abstract
Soilless cultivation has emerged as a sustainable solution for modern agriculture, yet substrate formulation is still often guided by empirical approaches, limiting efficiency and reproducibility. To address this gap, we established a data-driven framework for optimizing substrate composition in garden lettuce (Lactuca [...] Read more.
Soilless cultivation has emerged as a sustainable solution for modern agriculture, yet substrate formulation is still often guided by empirical approaches, limiting efficiency and reproducibility. To address this gap, we established a data-driven framework for optimizing substrate composition in garden lettuce (Lactuca sativa L.) cultivation. Using a randomized design, 200 substrate formulations were prepared from peat, vermiculite, and perlite, and their effects on plant growth were evaluated under controlled environmental conditions. Peat content reduced substrate porosity and water-holding capacity, whereas vermiculite increased both properties (linear regression, p < 0.05). Substrate formulations profoundly affected plant biomass, and the peat content was identified as a key predictor. Two rounds of substrate optimization resulted in a significant increase in shoot and root biomass and chlorophyll content, with increases of 57.5% (p = 9.2 × 10−8), 89.8% (p = 8.24 × 10−10), and 43.3% (p < 2 × 10−16), respectively, compared with the initial trial. Additionally, hyperspectral imaging (HSI) and RGB imaging were employed for growth monitoring. Random forest machine-learning method identified several red-edge indices (NDVI705, mNDVI705, mSR705) as highly responsive predictors of substrate formulations, highlighting the potential of imaging traits as proxies for substrate optimization. This study provides a reproducible pathway for improving soilless substrate formulations, contributing to data-informed substrate design and advancing the practice of precision agriculture. Full article
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35 pages, 3108 KB  
Review
Data-Driven Optimization of Discontinuous and Continuous Fiber Composite Processes Using Machine Learning: A Review
by Ivan Malashin, Dmitry Martysyuk, Vadim Tynchenko, Andrei Gantimurov, Vladimir Nelyub and Aleksei Borodulin
Polymers 2025, 17(18), 2557; https://doi.org/10.3390/polym17182557 - 22 Sep 2025
Viewed by 1226
Abstract
This paper surveys the application of machine learning in fiber composite manufacturing, highlighting its role in adaptive process control, defect detection, and real-time quality assurance. First, the need for ML in composite processing is highlighted, followed by a review of data-driven approaches—including predictive [...] Read more.
This paper surveys the application of machine learning in fiber composite manufacturing, highlighting its role in adaptive process control, defect detection, and real-time quality assurance. First, the need for ML in composite processing is highlighted, followed by a review of data-driven approaches—including predictive modeling, sensor fusion, and adaptive control—that address material heterogeneity and process variability. An in-depth analysis examines six case studies, among which are XPBD-based surrogates for RL-driven robotic draping, hyperspectral imaging (HSI) with U-Net segmentation for adhesion prediction, and CNN-driven surrogate optimization for variable-geometry forming. Building on these insights, a hybrid AI model architecture is proposed for natural-fiber composites, integrating a physics-informed GNN surrogate, a 3D Spectral-UNet for defect segmentation, and a cross-attention controller for closed-loop parameter adjustment. Validation on synthetic data—including visualizations of HSI segmentation, graph topologies, and controller action weights—demonstrates end-to-end operability. The discussion addresses interpretability, domain randomization, and sim-to-real transfer and highlights emerging trends such as physics-informed neural networks and digital twins. This paper concludes by outlining future challenges in small-data regimes and industrial scalability, thereby providing a comprehensive roadmap for ML-enabled composite manufacturing. Full article
(This article belongs to the Special Issue Artificial Intelligence in Polymers)
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19 pages, 4445 KB  
Article
Hyperspectral Imaging-Based Deep Learning Method for Detecting Quarantine Diseases in Apples
by Hang Zhang, Naibo Ye, Jingru Gong, Huajie Xue, Peihao Wang, Binbin Jiao, Liping Yin and Xi Qiao
Foods 2025, 14(18), 3246; https://doi.org/10.3390/foods14183246 - 18 Sep 2025
Viewed by 776
Abstract
Rapid detection of quarantine diseases in apples is essential for import–export control but remains difficult because routine inspections rely on manual visual checks that limit automation at port scale. A fast, non-destructive system suitable for deployment at customs is therefore needed. In this [...] Read more.
Rapid detection of quarantine diseases in apples is essential for import–export control but remains difficult because routine inspections rely on manual visual checks that limit automation at port scale. A fast, non-destructive system suitable for deployment at customs is therefore needed. In this study, three common apple quarantine pathogens were targeted using hyperspectral images acquired by a close-range hyperspectral camera and analyzed with a convolutional neural network (CNN). Symptoms of these diseases often appear similar in RGB images, making reliable differentiation difficult. Reflectance from 400 to 1000 nm was recorded to provide richer spectral detail for separating subtle disease signatures. To quantify stage-dependent differences, average reflectance curves were extracted for apples infected by each pathogen at early, middle, and late lesion stages. A CNN tailored to hyperspectral inputs, termed HSC-Resnet, was designed with an increased number of convolutional channels to accommodate the broad spectral dimension and with channel and spatial attention integrated to highlight informative bands and regions. HSC-Resnet achieved a precision of 95.51%, indicating strong potential for fast, accurate, and non-destructive detection of apple quarantine diseases in import–export management. Full article
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17 pages, 2726 KB  
Article
Genome-Wide Association Study of Chlorophyll Fluorescence and Hyperspectral Indices in Drought-Stressed Young Plants in Maize
by Lovro Vukadinović, Vlatko Galić, Maja Mazur, Antun Jambrović and Domagoj Šimić
Genes 2025, 16(9), 1068; https://doi.org/10.3390/genes16091068 - 11 Sep 2025
Viewed by 569
Abstract
Background/Objectives: Global maize production is considerably affected by drought aggravated by climate change. No genome-wide association study (GWAS) or candidate gene analysis has been performed using chlorophyll fluorescence (ChlF) and hyperspectral (HS) indices measured in young plants challenged by a water deficit. Our [...] Read more.
Background/Objectives: Global maize production is considerably affected by drought aggravated by climate change. No genome-wide association study (GWAS) or candidate gene analysis has been performed using chlorophyll fluorescence (ChlF) and hyperspectral (HS) indices measured in young plants challenged by a water deficit. Our objective was to conduct a GWAS of nine ChlF and HS indices measured in a diversity panel of drought-stressed young plants grown in a controlled environment using a maize single nucleotide polymorphism (SNP) 50k chip. Methods: A total of 165 inbred lines were genotyped using the Infinium Maize50K SNP array and association mapping was carried out using a mixed linear model. Results: The GWAS detected 37 respective SNP markers significantly associated with the maximum quantum yield of the primary photochemistry of a dark-adapted leaf (Phi_Po), the probability that a trapped exciton moves an electron into the electron transport chain further than QA (Psi_o), the normalized difference vegetation index (NDVI), the Zarco–Tejada and Miller Index (ZMI), greenness, modified chlorophyll absorption in reflectance (MCARI), modified chlorophyll absorption in reflectance 1 (MCARI1), and Gitelson and Merzlyak indices 1 and 2 (GM1 and GM2). Conclusions: Our results contribute to a better understanding of the genetic dissection of the ChlF and HS indices, which is directly or indirectly related to physiological processes in maize, supporting the use of HS imaging in the context of maize breeding. Full article
(This article belongs to the Special Issue Molecular Breeding and Genetics of Plant Drought Resistance)
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19 pages, 3987 KB  
Article
Rapid Identification of Dendrobium Species Using Near-Infrared Hyperspectral Imaging Technology
by Kaixuan Li, Yijun Guo, Haosheng Zhong, Yiqi Jin, Bin Li, Huimin Fang, Lijian Yao and Chao Zhao
Sensors 2025, 25(18), 5625; https://doi.org/10.3390/s25185625 - 9 Sep 2025
Cited by 1 | Viewed by 616
Abstract
Dendrobium officinale is a valuable Chinese medicinal herb, but distinguishing it from other Dendrobium species after processing is challenging, leading to low classification accuracy and time-consuming analysis. This study proposes a rapid classification model based on near-infrared hyperspectral imaging (NIR-HSI), incorporating data preprocessing [...] Read more.
Dendrobium officinale is a valuable Chinese medicinal herb, but distinguishing it from other Dendrobium species after processing is challenging, leading to low classification accuracy and time-consuming analysis. This study proposes a rapid classification model based on near-infrared hyperspectral imaging (NIR-HSI), incorporating data preprocessing and feature wavelength selection. Five Dendrobium species—D. officinale, D. aphyllum, D. chrysanthum, D. fimbriatum, and D. thyrsiflorum—were used. Spectral preprocessing techniques like normalization and smoothing were applied, and Support Vector Machine (SVM) models were constructed. Normalization improved both accuracy and stability, with the full-spectrum Normalize-SVM model achieving 97% accuracy for calibration and 88% for prediction. D. chrysotoxum performed best, with all metrics reaching 100%, while D. aphyllum had poor classification (40% recall and 51.74% F1 score). To improve efficiency and performance, feature wavelength selection was performed using Competitive Adaptive Reweighted Sampling (CARS) and Successive Projections Algorithm (SPA). The CARS-Normalize-SVM model yielded the best results: 98% accuracy for calibration and 96% for prediction, improving by 1% and 8%, respectively. D. aphyllum’s classification also improved significantly, with a 100% recall rate and 95.24% F1 score. These findings highlight hyperspectral imaging’s potential for rapid Dendrobium species identification, supporting future quality control and market supervision. Full article
(This article belongs to the Special Issue Recent Advances in Spectroscopic Sensing and Sensor Engineering)
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