Search Results (57)

This study provides a comprehensive database of sulfur isotope values from Greece, including samples of C3 and C4 plants and terrestrial and aquatic animal bones. This comprehensive analytical approach examines sulfur isotopes—along with carbon and nitrogen—in modern plants, terrestrial mammals, and fish bones (fresh and marine reservoirs) from Greece. The results show a clear offset in δ³⁴S values between terrestrial and aquatic animals, influenced by their dietary sources from marine or freshwater environments. This δ³⁴S offset and the clear difference between S-C-N isotopes permits the reconstruction of the dietary habits of domesticated herbivores and demonstrates differences in husbandry practices and animal movements. Additionally, the combination of sulfur and nitrogen values allows the reconstruction of the diet of omnivores, revealing the type of protein consumed. Finally, this isotopic dataset will provide an essential backbone for future archaeological, ecological, and forensic studies. Full article

This study utilized data from 300 prefecture-level cities in China, spanning from 2000 to 2020, and employed a difference-in-differences (DID) model to investigate the influence of land development rights on agricultural land prices, alongside the mechanisms underlying this relationship. The primary aim of this research was to analyze the manner in which land development rights affect agricultural land prices through the implementation of policies and market forces. Via empirical analysis, the study elucidated the effects of land development rights on agricultural land prices within China. The key findings include the following: (1) Land development rights positively influence the increase in agricultural land prices. (2) Land development rights significantly narrow the urban–rural income disparity at municipal and county levels, which in turn impacts agricultural land prices. (3) The effect of land development rights on agricultural land prices is negatively moderated by regional economic growth. (4) While land development rights significantly enhance the prices of arable land, their impact on sectors such as agriculture, forestry, animal husbandry, fishing, and food processing remains minimal. (5) In northern regions and economically underdeveloped areas, land development rights substantially boost agricultural land prices, underscoring their role in fostering local economic development and enhancing land use efficiency. Full article

Crossbreeding is a traditional breeding technique and has been performed successfully in many fish species. However, distant hybridization between different genera is hard to be successful because of reproductive isolation. In this study, diploid hybrids (PL) were successfully derived from the hybridization of Ussuri catfish (Pseudobagrus ussuriensis, PU, ♀, 2n = 52) and longsnout catfish (Leiocassis longirostris, LL, ♂, 2n = 52). And the morphological data, external frame parameters, chromosomal karyotypes, DNA content measurement, mitochondrial DNA control region, and species-specific marker identification were applied to investigate the traits and genetic characterization of the PL hybrid offspring and their parents. Both quantifiable traits and shape frame parameters of the PL hybrid offspring were revealed to be intermediate between those of their parents; however, cluster analysis showed that their external morphology was more in favor of the maternal PU. The growth comparisons showed that the hybrids had significant growth advantages over maternal PU. Based on karyotype patterns, DNA contents and mitochondrial DNA, the hybrid origin and maternal inheritance of hybrid offspring were further confirmed. According to the sequence variations identified from the genome sequences of the two catfish species, one species-specific marker was developed to distinguish the PL hybrid offspring and their parents. Therefore, this study provides a successful case for intergeneric hybridization and hybrid superiority, and the PL hybrid shows promise for commercial application, pending further studies into its husbandry, health, and welfare with larger populations of fish. Full article

This study investigated the effect of partially replacing fish meal with krill and copepod meals in inert diets co-fed with shrimp on the growth and nutrient digestibility of long-snout seahorses (Hippocampus guttulatus). A control diet (Diet 1) using raw starch and four experimental diets with similar protein (≈44.8%) and energy (≈15.1 MJ/kg) levels were tested. Diet 2 used fish meal as the sole protein source, while in Diets 3–5, krill and copepod meals replaced 44% of the fish meal. Seahorses fed shrimp + Diets 2–5 showed significantly higher growth rates (p < 0.05) than those fed shrimp + Diet 1, though there were no significant growth differences among Diets 2–5. Digestibility of dry matter (46.1% to 72.2%), lipids (73.3% to 85.5%), crude protein (89.8% to 95.8%), energy (82% to 92.2%), and phosphorus (28.7% to 64.4%) varied with diet, being consistently lower in seahorses fed shrimp + Diet 1. As an agastric species, H. guttulatus did not exhibit impaired digestibility for any of the tested nutrients, minerals, or energy. This study suggests that crustacean meals can effectively substitute fish meal in inert diets for this species, contributing to the sustainability and optimization of captive seahorse husbandry practices. Full article

Agriculture is a critical foundation for the development of large-scale complex and urban societies. Sanxingdui, located on the Chengdu Plain in western Sichuan and linked with the ancient Chinese Shu Kingdom, is one of the most distinctive archaeological sites in the world. However, despite its importance, the agricultural economy of the Sanxingdui culture and the Chengdu Plain remains poorly understood and heavily debated. This study synthesizes recent archaeological and historical evidence concerning the agricultural economy underpinning the increasing scale and social complexity at Sanxingdui and the greater Chengdu Plain prior to 2200 BP. Our analysis finds a mixed rice and dry-land millet farming economy, in conjunction with pig and chicken husbandry, during the Sanxingdui culture (3700–3100 BP). This integrated agricultural system likely began with the Baodun culture (4500–3700 BP), passing to the Sanxingdui culture (3700–3100 BP), Shierqiao culture (3100–2600 BP) and later, the Shu culture (2600–2300 BP). In addition, although gathering, hunting, and fishing accounted for relatively low proportions of the overall subsistence, the ancient peoples at Sanxingdui and across the Chengdu Plain continued these practices, supporting a diverse agricultural and food system. Understanding the subsistence at Sanxingdui offers key insights into the development of complex societies in southwest China, the contributions to Chinese culture, and the role of agriculture worldwide. Full article

Postbiotics are innovative tools in animal husbandry, providing eco-friendly solutions for disease management within the industry. In this study, a new postbiotic product was evaluated for its impact on the health of rainbow trout (Oncorhynchus mykiss). In vivo studies were conducted to assess the safety of the Weissella cibaria strains used in postbiotic production. Additionally, this study evaluated the impact of diet supplementation with 0.50% postbiotics on growth performance during a 30-day feeding trial; the gut microbial communities, immunomodulation, and protection against Yersinia ruckeri infection were evaluated. The strains did not harm the animals during the 20-day observation period. Furthermore, the effect of postbiotics on growth performance was not significant (p < 0.05). The treated group showed a significant increase in acid-lactic bacteria on the 30th day of the feeding trial, with counts of 3.42 ± 0.21 log CFU/mL. Additionally, there was an up-regulation of the pro-inflammatory cytokine IL-1β in head kidney samples after 48 h of feed supplementation, whereas cytokines IL-10, IL-8, INF-γ, and TNF-α were down-regulated. The findings indicate that rainbow trout fed with postbiotics saw an improvement in their survival rate against Y. ruckeri, with a 20.66% survival improvement in the treated group. This study proves that incorporating postbiotics from two strains of W. cibaria previously isolated from rainbow trout into the diet of fish has immunomodulatory effects, enhances intestinal microbial composition, and improves fish resistance against Y. ruckeri. Full article

Introduction: Elasmobranchs currently constitute an important part of the animal collection of many aquariums worldwide. Their maintenance under human care has allowed us to describe and identify new pathogens and diseases affecting them, as well as to determine different treatments for these diseases. Great advances in elasmobranch husbandry have been developed. Methods: A search was performed on scientific databases as PubMed and other specialized sources (IAAAM archive). Results: Little information on pharmacotherapeutics is available in this taxonomic group, and treatments lack a scientific base and instead are frequently dependent on empirical knowledge. Pharmacokinetic studies are the first step to determining therapeutic protocols that are safe and effective. The available bibliography shows that a majority of the mycoses recorded in cartilaginous fish are severe, aggravated by the fact that the antifungal treatments administered, following the guidelines used for teleost species, are ineffective in elasmobranchs. Azoles appear to be a promising group of antifungals for use in treating systemic mycoses in sharks and rays. Conclusions: Based on the findings of this review, it is essential to investigate the pharmacokinetics of the different antifungals in these species in order to provide therapeutic options for fungal infections in cartilaginous fish. Full article

The present study was applied to evaluate the effects of alternate feeding and feed restriction on gene expression, growth, proximate composition and biochemical indices in European seabass, Dicentrarchus labrax. Fish were randomly divided into six indoor tanks with 90 fish per tank in a recirculating aquaculture system. Two feeding strategies were applied, in which the first group was fed daily to satiation and the second was intermittently fed (8 days feeding to satiation–2 days starvation) for 40 days. At the end of the experiment, outlier fish were sorted as fast growers (FG) and slow growers (SG) according to their final body weight. The differential gene expression tested was related to glycolysis (pk, ldha, hk, g3pdh, eno1 and alda), fatty acid metabolism (lpl and acc) and cholesterol synthesis (7dhcr and sqle). In addition, muscle ldha and gpi expressions were positively correlated with fish weight. The concentrations of glucose, triglycerides, cholesterol and non-esterified fatty acids (NEFA) were not affected by the dietary treatments. Glucose and NEFA differed significantly between SG and FG fed groups. Overall, the physiological responses of glucose and fatty acid metabolism in fish, as recorded by gene expression assays, were triggered by minor interventions in feeding rather than the different growth rates. Expression of specific genes and biochemical parameters could be used as potential biomarkers to improve aquaculture practices and benefit fish husbandry through selective breeding, feeding strategies and farm management. The study provides new insights on the impact of intermittent feeding of European seabass, with gene markers and their potential effects, for European seabass aquaculture. Full article

Anabantidae is a large and diverse group of fish cultured both under aquaculture conditions and as a hobby. These fish share a common structural feature in the form of an additional respiratory organ. Despite the enormous availability of these fish worldwide, little is known about their feeding preferences in husbandry and their influence on homeostasis under both industrial and domestic conditions. This study describes, for the first time, the structure of the digestive tracts of two Anabantoidei fishes: African bush fish (Ctenopoma acutirostre) and paradise fish (Macropodus opercularis). The overall structure of the digestive tract and its histological structure were analyzed and compared in both fish species. Physiological predispositions indicated a predominance of omnivorous fish traits in M. opercularis in contrast to C. acutirostre, which has several morphological traits indicating greater adaptation to carnivory, particularly ichthyophagy. The results obtained will allow further research to be conducted in the future to optimize the nutrition and feeding of these fish and to develop appropriate dietary recommendations. Full article

A wide variety of fish species have been displayed in public aquariums and zoological collections for over 150 years. Though the issue of pain perception in fish is still being debated, there is no disagreement that negative impacts on their welfare can significantly affect their health and wellbeing. A general description of the basic biological requirements for maintaining fish in captive environments is presented, but species-specific information and guidelines should be developed for the multitude of species being maintained. A combination of behavioral, performance, and physiological indicators can be used to assess the well-being of these animals. Ultimately, the goal for optimizing the welfare of fish should be to provide the best possible environment, husbandry, and social interactions to promote natural species-specific behaviors of the fish in captivity. Full article

Heat shock proteins (HSPs) encompass both extrinsic chaperones and stress proteins. These proteins, with molecular weights ranging from 14 to 120 kDa, are conserved across all living organisms and are expressed in response to stress. The upregulation of specific genes triggers the synthesis of HSPs, facilitated by the interaction between heat shock factors and gene promoter regions. Notably, HSPs function as chaperones or helper molecules in various cellular processes involving lipids and proteins, and their upregulation is not limited to heat-induced stress but also occurs in response to anoxia, acidosis, hypoxia, toxins, ischemia, protein breakdown, and microbial infection. HSPs play a vital role in regulating protein synthesis in cells. They assist in the folding and assembly of other cellular proteins, primarily through HSP families such as HSP70 and HSP90. Additionally, the process of the folding, translocation, and aggregation of proteins is governed by the dynamic partitioning facilitated by HSPs throughout the cell. Beyond their involvement in protein metabolism, HSPs also exert a significant influence on apoptosis, the immune system, and various characteristics of inflammation. The immunity of aquatic organisms, including shrimp, fish, and shellfish, relies heavily on the development of inflammation, as well as non-specific and specific immune responses to viral and bacterial infections. Recent advancements in aquatic research have demonstrated that the HSP levels in populations of fish, shrimp, and shellfish can be increased through non-traumatic means such as water or oral administration of HSP stimulants, exogenous HSPs, and heat induction. These methods have proven useful in reducing physical stress and trauma, while also facilitating sustainable husbandry practices such as vaccination and transportation, thereby offering health benefits. Hence, the present review discusses the importance of HSPs in different tissues in aquatic organisms (fish, shrimp), and their expression levels during pathogen invasion; this gives new insights into the significance of HSPs in invertebrates. Full article

This study aims to contribute to the knowledge of the medieval Moldovan economy by evaluating animal resources (e.g., animal husbandry, hunting, fishing) based on the skeletal remains found in archaeologic sites from northeastern Romania and the Republic of Moldova. Animal remains, especially those from the urban settlement of the 14th–16th centuries from Târgu Neamţ (NE Romania), were described in terms of their frequencies (i.e., number of identified specimens and minimum number of individuals), morphometry, and livestock management (i.e., animal selection by age and sex). The results were compared with those obtained from other settlements—rural, urban, and fortress—from medieval Moldova. Correspondence analysis of the identified animals and settlements on the basis of the frequency values reveals associations between the two variables (animal species and settlement). Full article

It is crucial that simple and high-throughput methods for determining multiple, or groups of, sulfonamides (SAs) be developed since they are widely used in animal husbandry and aquaculture. We developed a paper-based multicolor colorimetric aptasensor to detect 3 SAs: sulfaquinoxaline (SQ), sulfamethoxypyridazine (SMP) and sulfamethoxydiazine (SMD). Using a broad-specificity aptamer as a bioreceptor, we reduced the growth of nicotinamide adenine dinucleotide I (NADH)–ascorbic acid (AA)-mediated gold nanobipyramids (AuNBPs) to generate a multicolor signal. We also used a paper-based analytical device (PAD) system to deposit AuNBPs for a sensitive color signal read out. The aptasensor can detect more color changes corresponding to the concentrations of SQ, SMP and SMD and has higher sensitivity, better specificity and stability. It can also be used to determine SQ, SMP and SDM individually, or collectively, or any two together with a visual detection limit of 0.3–1.0 µM, a spectrometry quantification limit (LOQ) of 0.3–0.5 µM and a spectrometry detection limits (LOD) of 0.09–0.15 µM. The aptasensor was successfully used to determine SQ, SMP and SDM in fish muscle with a recovery of 89–94% and a RSD n = 5) < 8%, making it a promising method for the rapid screening of total SQ, SMP and SDM residue in seafood. Full article

Ornamental fish trade represents an important economic sector with an export turnover that reached approximately 5 billion US dollars in 2018. Despite its high economic importance, this sector does not receive much attention. Ornamental fish husbandry still faces many challenges and losses caused by transport stress and handling and outbreak of diseases are still to be improved. This review will provide insights on ornamental fish diseases along with the measures used to avoid or limit their onset. Moreover, this review will discuss the role of different natural and sustainable microbial feed additives, particularly probiotics, prebiotics, and synbiotics on the health, reduction in transport stress, growth, and reproduction of farmed ornamental fish. Most importantly, this review aims to fill the informational gaps existing in advanced and sustainable practices in the ornamental fish production. Full article

Spatially heterogeneous turbulent flow refers to nonuniform flow with coexisting multiple flow velocities, which is widely distributed in fish natural or husbandry environments, and its hydraulic parameters affect fish swimming behavior. In this study, a complex hydrodynamic environment with three flow velocity regions (low, medium, and high) coexisting in an open-channel flume was designed to explore volitional swimming ability, the spatial-temporal distribution of fish swimming trajectories, and the range of preferred hydrodynamic parameters of Schizothorax prenanti individual and schooling (three fish). The results showed that the swimming speed of individual fish during upstream migration was significantly higher than that of fish schools (p < 0.05). The swimming trajectories of fish schooling showed that they spent more time synchronously exploring the flow environment during upstream migration compared with individual fish. By superimposing the fish swimming trajectories on the environmental flow field, the range of hydrodynamic environments preferred by fish in complex flow fields was quantified. This research provides a novel approach for investigating the natural swimming behavior of fish species, and a theoretical reference for the restoration of fish natural habitats or flow enrichment of husbandry environments. Full article