Search Results (34)

This article presents the wear characteristics of the working surface of WC-Co (Tungsten Carbide–Cobalt) tungsten carbide tools obtained using the innovative U-FAST (Upgraded Field-Assisted Sintering Technology) method for particleboard machining. Three groups of tools with a similar chemical composition but differing WC (Tungsten Carbide) grain sizes were tested. Milling tests were carried out on a CNC (Computer Numerical Control) machine tool with the following cutting parameters: spindle rotation at 15,000 rpm, a feed rate of 0.25 mm per tooth, and a feed rate of 3.75. The experimental results show that tools with submicron WC grit sizes of 0.4 µm and 0.8 µm have the longest tool life. Wear of the cutting edges occurred through the removal of the cobalt bond between the tungsten carbide grains, leading to fracture and mechanical removal of the grains from the cutting edge surface. The similarities in the relative wear characteristics of blades with submicron tungsten carbide grain sizes suggest that micro-abrasion and bond phase extrusion may be the main wear mechanisms under the experimental conditions. Nanometric WC grain size significantly influences tool wear through chipping and cracking. Full article

Tool breakage in CNC machining often leads to reduced productivity and increased maintenance costs. This study proposes a non-contact tool breakage detection method using spindle current signals captured by an SCT013 current sensor. The sensor easily attaches to the motor line without any hardware modification and provides real-time current signals for frequency domain analysis. Fast Fourier Transform (FFT) is employed to extract spectral features, particularly focusing on high-frequency energy spikes at the moment of breakage. A total of 20 experiments were conducted, and consistent spectral anomalies were observed. Additionally, deep learning models including ANN, DNN, and CNN were compared for automated detection performance. The results indicate that the proposed system can reliably detect tool breakage by identifying frequency domain anomalies that emerge within 1–3 s after the actual event, based on processed current signals. While the inference time of deep learning models ranges from 15 to 58 s, the detection mechanism captures the breakage characteristics early in the signal, enabling timely tool condition evaluation. Full article

In CNC machines, the temperature field analysis of spindle servo permanent magnet motors (SSPMMs) under rated load, overload, and weak magnetic conditions is critical for ensuring stable operation and machining accuracy. This paper proposes a temperature calculation method for SSPMMs based on the thermal network method, which is used to quickly evaluate the temperature performance of SSPMMs under different operating conditions during design. This method can calculate the steady-state or transient temperature rise under different operating conditions. First, the electromagnetic performance and heat sources of the SSPMMs were analyzed. Then, based on the thermal network method, the equivalent thermal resistances and equivalent heat dissipation coefficients of the motor components were calculated. By iterating the heat balance equation or solving the heat conduction equation for different operating conditions, the temperature distribution of SSPMMs under different operating conditions was obtained. The accuracy of the thermal network model was validated through temperature analysis using fluid–structure interaction simulations and prototype testing. The results show that the relative error between the winding temperature calculated by the proposed equivalent thermal network model and the measured temperature under different operating conditions is less than 5%. This paper provides a theoretical basis for the thermal management of SSPMM, which can quickly and accurately evaluate the temperature rise in the motor during design. Full article

Virtual simulation of high-resolution multi-axis machining processes nowadays plays an important role in the production of complex parts in various industries. In order to improve the surface quality and productivity, process parameters, such as spindle speed, feedrate, and depth of cut, need to be optimized by using an accurate process model of milling, which requires both the fast virtual prototyping of machined part geometry for tool path verification and accurate determination of cutter–workpiece engagement for cutting force predictions. Under these circumstances, this paper presents an effective volumetric method that can accurately provide the required geometric information with high and stable computational efficiency under the condition of high grid resolution. The proposed method is built on a tri-level grid, which applies two levels of adaptive refinement in space decomposition to abolish the adverse effect of a large fine-level branching factor on its efficiency. Since hierarchical space decomposition is used, this multi-level representation enables the batch processing of affected voxels and minimal intersection calculations, achieving fast and accurate modeling results. To calculate the instantaneous engagement region, the immersion angles are obtained by fusing the intersection points between the bottom-level voxel edges and the cutter surface, which are then trimmed by feasible contact arcs determined using envelope theory. In a series of test cases, the proposed method shows higher efficiency than the tri-dexel model and stronger applicability in high-precision machining than the two-level grid. Full article

We used numerical methods to define the normative structure of the different stages of sleep and wake (W) in a pilot study of 19 participants without pathology (18–64 years old) using a double-banana bipolar montage. Artefact-free 120–240 s epoch lengths were visually identified and divided into 1 s windows with a 10% overlap. Differential channels were grouped into frontal, parieto-occipital, and temporal lobes. For every channel, the power spectrum (PS) was calculated via fast Fourier transform and used to compute the areas for the delta (0–4 Hz), theta (4–8 Hz), alpha (8–13 Hz), and beta (13–30 Hz) bands, which were log-transformed. Furthermore, Pearson’s correlation coefficient and coherence by bands were computed. Differences in logPS and synchronization from the whole scalp were observed between the sexes for specific stages. However, these differences vanished when specific lobes were considered. Considering the location and stages, the logPS and synchronization vary highly and specifically in a complex manner. Furthermore, the average spectra for every channel and stage were very well defined, with phase-specific features (e.g., the sigma band during N2 and N3, or the occipital alpha component during wakefulness), although the slow alpha component (8.0–8.5 Hz) persisted during NREM and REM sleep. The average spectra were symmetric between hemispheres. The properties of K-complexes and the sigma band (mainly due to sleep spindles—SSs) were deeply analyzed during the NREM N2 stage. The properties of the sigma band are directly related to the density of SSs. The average frequency of SSs in the frontal lobe was lower than that in the occipital lobe. In approximately 30% of the participants, SSs showed bimodal components in the anterior regions. qEEG can be easily and reliably used to study sleep in healthy participants and patients. Full article

This research conducts a comprehensive comparative analysis of simulation methodologies for spindle pumps, with a specific focus on steady-state CFD, transient-CFD, and lumped-parameter approaches. Spindle pumps, renowned for their reliability, efficiency, and low noise emission, play a pivotal role in Thermal Management for Battery Electric Vehicles, aligning with the automotive industry’s commitment to reducing pollutants and CO₂ emissions. The study is motivated by the critical need to curtail energy consumption during on-the-road operations, particularly as the automotive industry strives for enhanced efficiency. While centrifugal pumps are commonly employed for such applications, their efficiency is highly contingent on rotational speed, leading to energy wastage in real-world scenarios despite high efficiency at the design point. Consequently, the adoption of precisely designed spindle pumps for thermal management systems emerges as a viable solution to meet evolving industry needs. Recognizing the profound impact of simulation tools on the design and optimization phases for pump manufacturers, this research emphasizes the significance of fast and accurate simulation tools. Transient-CFD emerges as a powerful Tool, enabling real-time monitoring of various performance indicators, while steady-CFD, with minimal simplifications, adeptly captures pressure distribution and machine leakages. Lumped-parameter approaches, though requiring effort in simulation setup and simplifying input geometry, offer rapid computational times and comprehensive predictions, including leakages, Torque, cavitation, and pressure ripple. Breaking new ground, this paper presents, for the first time in the literature, accurate simulation models for the same reference machine using the aforementioned methodologies. The results were rigorously validated against experiments spanning a wide range of pump speeds and pressure drops. The discussion encompasses predicted flow, Torque, cavitation, and pressure ripple, offering valuable insights into the strengths and limitations of each methodology. Full article

Globally, women have been adopting oocyte cryopreservation (OC) for fertility preservation for various reasons, such as inevitable gonadotoxic treatment for specific pathologic states and social preferences. While conventional vitrification (C-VIT) has improved the success rate of OC, challenges of possible toxicities of high-concentration cryoprotective agents and osmotic stress persist. To overcome these challenges, we evaluated the ultra-fast vitrification (UF-VIT) method, which reduces the equilibration solution stage exposure time compared to C-VIT by observing mouse oocyte intracellular organelles and embryonic development. Consequently, compared to fresh mouse oocytes, UF-VIT presented significant differences only in endoplasmic reticulum (ER) intensity and mitochondrial (MT) distribution. Meanwhile, C-VIT showed substantial differences in the survival rate, key ER and MT parameters, and embryonic development rate. UF-VIT exhibited considerably fewer negative effects on key MT parameters and resulted in a notably higher blastocyst formation rate than C-VIT. Meiotic spindle (spindle and chromosomes) morphology showed no significant changes between the groups during vitrification/warming (VW), suggesting that VW did not negatively affect the meiotic spindle of the oocytes. In conclusion, UF-VIT seems more effective in OC owing to efficient cytoplasmic water molecule extraction, osmotic stress reduction, and minimization of cell contraction and expansion amplitude, thus compensating for the drawbacks of C-VIT. Full article

The post-COVID-19 condition is defined by the World Health Organization as the persistence of symptoms or development of new symptoms three months after the initial SARS-CoV-2 infection, lasting for at least two months without a clear explanation. Neuropsychiatric disorders associated with this condition include asthenia, memory and concentration problems, and sleep disturbances. Our study aims to investigate sleep patterns following SARS-CoV-2 infection using EEG findings and a sleep quality questionnaire completed by parents (Sleep Disturbance Scale for Children—SDSC). Notably, our investigation is based on a convenience sample. The patients in our sample, aged 1 to 14 years, are not currently taking any medications; rather, they are undergoing follow-up assessments at the Child Neuropsychiatry department of the University Hospital of Messina for neurodevelopmental evaluations. Specifically, we are analyzing amplitude and power spectrum data in the first five minutes of NREM2 sleep, calculated from EEG recordings obtained via bipolar leads within three months after the onset of the disease. These results will be compared with controls performed on the same subjects in the six months preceding the infection. The focus of the study was sleep spindles, which are generated by the thalamocortical systems and play a role in sleep modulation, memory, and learning. Preliminary analysis suggests a predominant increase in the slow component of the spindles in the right-frontal lead. Full article

To explore the processes of epileptogenesis/ictogenesis, this study determined the age-dependent development of the functional abnormalities in astroglial transmission associated with pannexin1-hemichannel using a genetic rat model of autosomal dominant sleep-related hypermotor epilepsy (ADSHE) named ‘S286L-TG’. Pannexin1 expression in the plasma membrane of primary cultured cortical astrocytes and the orbitofrontal cortex (OFC), which is an ADSHE focus region, were determined using capillary immunoblotting. Astroglial D-serine releases induced by artificial high-frequency oscillation (HFO)-evoked stimulation, the removal of extracellular Ca²⁺, and the P2X7 receptor agonist (BzATP) were determined using ultra-high performance liquid chromatography (UHPLC). The expressions of pannexin1 in the plasma membrane fraction of the OFC in S286L-TG at four weeks old were almost equivalent when compared to the wild type. The pannexin1 expression in the OFC of the wild type non-statistically decreased age-dependently, whereas that in S286L-TG significantly increased age-dependently, resulting in relatively increasing pannexin1 expression from the 7- (at the onset of interictal discharge) and 10-week-old (after the ADSHE seizure onset) S286L-TG compared to the wild type. However, no functional abnormalities of astroglial pannexin1 expression or D-serine release through the pannexin1-hemichannels from the cultured astrocytes of S286L-TG could be detected. Acutely HFO-evoked stimulation, such as physiological ripple burst (200 Hz) and epileptogenic fast ripple burst (500 Hz), frequency-dependently increased both pannexin1 expression in the astroglial plasma membrane and astroglial D-serine release. Neither the selective inhibitors of pannexin1-hemichannel (10PANX) nor connexin43-hemichannel (Gap19) affected astroglial D-serine release during the resting stage, whereas HFO-evoked D-serine release was suppressed by both inhibitors. The inhibitory effect of 10PANX on the ripple burst-evoked D-serine release was more predominant than that of Gap19, whereas fast ripple burst-evoked D-serine release was predominantly suppressed by Gap19 rather than 10PANX. Astroglial D-serine release induced by acute exposure to BzATP was suppressed by 10PANX but not by Gap19. These results suggest that physiological ripple burst during the sleep spindle plays important roles in the organization of some components of cognition in healthy individuals, but conversely, it contributes to the initial development of epileptogenesis/ictogenesis in individuals who have ADSHE vulnerability via activation of the astroglial excitatory transmission associated with pannexin1-hemichannels. Full article

The rapid advancement of genetic technologies has made it possible to modify various plants through both genetic transformation and gene editing techniques. Poplar, with its rapid in vitro growth and regeneration enabling high rates of micropropagation, has emerged as a model system for the genetic transformation of woody plants. In this study, Populus × berolinensis K. Koch. (Berlin poplar) was chosen as the model organism due to its narrow leaves and spindle-shaped crown, which make it highly suitable for in vitro manipulations. Various protocols for the Agrobacterium-mediated transformation of poplar species have been developed to date. However, the genetic transformation procedures are often constrained by the complexity of the nutrient media used for plant regeneration and growth, which could potentially be simplified. Our study presents a cheaper, simplified, and relatively fast protocol for the Agrobacterium-mediated transformation of Berlin poplar. The protocol involved using internode sections without axillary buds as explants, which were co-cultivated in 10 µL droplets of bacterial suspension directly on the surface of a solid agar-based medium without rinsing and sterile paper drying after inoculation. We used only one regeneration Murashige and Skoogbased medium supplemented with BA (0.2 mg·L⁻¹), TDZ (0.02 mg·L⁻¹), and NAA (0.01 mg·L⁻¹). Acetosyringone was not used as an induction agent for vir genes during the genetic transformation. Applying our protocol and using the binary plasmid pBI121 carrying the nptII selective and uidA reporter genes, we obtained the six transgenic lines of poplar. Transgenesis was confirmed through a PCR-based screening of kanamycin-selected regenerants for the presence of both mentioned genes, Sanger sequencing, and tests for detecting the maintained activity of both genes. The transformation efficiency, considering the 100 explants taken originally, was 6%. Full article

SiCp/Al composites are used in the aerospace, automotive, and electronics fields, among others, due to their excellent physical and mechanical properties. However, as they are hard-to-machine materials, poor surface quality has become a major limitation to their wider applications. To effectively control the quality of machined surfaces, it is necessary to accurately detect and characterize defects. Based on the YOLOv4 object detection algorithm, a SiCp/Al composite machined surface defect detection model has been developed for the accurate and fast detection of machined surface defects. OpenCV is used to process images of detected defects and extract defect feature parameters. The number of defects and the total defect area in the same machining area are used as evaluation criteria to assess the quality of the machined surface, and the effect of the machining parameters on the quality of the machined surface is analyzed. The results show that the number and total area of surface defects that occur when grinding SiCp/Al composites are positively correlated with the feed rate, tool diameter, and size of the abrasive, while they are negatively correlated with the spindle speed and ultrasonic vibration amplitude. When the grinding depth is greater than 20 microns, the quality of the machined surface is greatly affected. Full article

Milling with use of CNC machines is a well-established method and much research was concluded on this topic. However, when it comes to wood and wood composites, the material non-homogeneity brings a lot of variability into cutting conditions. As a part of research into potential signals for nesting milling, material vibrations at clamping points were examined in this study. The main goal was to conclude if cutting parameters have a statistically significant effect on measurement. The place of measurement was analyzed so it was accessible to the machine operator. Medium density fiberboard and particleboard specimens were cut through by razor and spiral mill, with spindle rotating 10,000 and 20,000 min⁻¹ and feed rates 2, 6, 10 m·min⁻¹. Vibrations were measured at vacuum grippers, and were then processed by fast Fourier transform. Then, frequency spectrum maxima were compared, as well as amplitude sizes. Main frequencies were of roughly 166 Hz and multiples, suggesting their origin in tool rotation. When maxima were compared, tool use, spindle rotation, and feed rate seemed to affect the result. Frequency spectrum amplitudes were subjected to analysis of variance, significant effect was found on spindle speed, tool, and specimen material. No significant effect was found with differing feed rates. Full article

Obstructive sleep apnea syndrome (OSAS) causes intermittent hypoxia and sleep disruption in the brain, resulting in cognitive dysfunction, but its pathogenesis is unclear. The sleep spindle wave is a transient neural event involved in sleep memory consolidation and synaptic plasticity. This study aimed to investigate the characteristics of sleep spindle activity and its relationship with memory ability in patients with OSAS. A total of 119 patients, who were divided into the OSAS group (n = 59, AHI ≥ 15) and control group (n = 60, AHI < 15) according to the Apnea Hypopnea Index (AHI), were enrolled and underwent polysomnography. Power spectral density (PSD) and omega complexity were used to analyze the characteristics of single and different brain regions of sleep spindles. Memory-related cognitive functions were assessed in all subjects, including logical memory, digit ordering, pattern recognition, spatial recognition and spatial working memory. The spindle PSD of the OSAS group was significantly slower than the control group, regardless of the slow, fast, or total spindle. The complexity of the spindles in the prefrontal and central region decreased significantly, whereas it increased in the occipital region. Sleep spindle PSD was positively correlated with logical memory and working memory. Spindle complexity was positively correlated with immediate logical and visual memory in the prefrontal region and positively correlated with immediate/delayed logical and working memory in the central region. In contrast, spindle complexity in the occipital region negatively correlated with delayed logical memory. Spindle hyperconnectivity in the prefrontal and central regions underlies declines in logical, visual and working memory and weak connections in the occipital spindles underlie the decline in delayed logical memory. Full article

The possibility of distinguishing insomniacs from good sleepers based on polysomnography (PSG) remains an open question. While these groups show modest differences in traditional PSG parameters, some studies suggest that finer measures may be more useful. Here we assess differences between good sleepers (GS), poor sleepers (PS) and insomniacs (IN) in classical PSG measures as well as in sleep continuity, stability and cyclic organization. PSG-monitored sleep (two nights) of 17 IN (diagnosed through a standard clinical interview; Pittsburgh Sleep Quality Index (PSQI) ≥ 5, Insomnia Severity Index (ISI) > 14) was compared to that of 33 GS (PSQI < 5) and 20 PS (PSQI ≥ 5, ISI ≤ 14). Compared to GS, IN were impaired in sleep macrostructure (sleep latency, sleep efficiency, WASO%) and in continuity, stability and organization, whereas PS only showed disrupted continuity and stability. Spindle parameters were comparable between IN and GS, but the former displayed enhanced power in fast frequency bands. Our findings support the hypothesis of a continuum between individuals with self-reported poor sleep and insomniacs. Further, they add to extant data on impaired sleep continuity, stability and organization in poor sleepers and elderly individuals, underlining the utility of including these measures in standard sleep assessments. Full article

This paper presents some advances in condition monitoring for rotary machines (particularly for a lathe headstock gearbox) running idle with a constant speed, based on the behaviour of a driving three-phase AC asynchronous induction motor used as a sensor of the mechanical power via the absorbed electrical power. The majority of the variable phenomena involved in this condition monitoring are periodical (machines having rotary parts) and should be mechanically supplied through a variable electrical power absorbed by a motor with periodical components (having frequencies equal to the rotational frequency of the machine parts). The paper proposes some signal processing and analysis methods for the variable part of the absorbed electrical power (or its constituents: active and instantaneous power, instantaneous current, power factor, etc.) in order to achieve a description of these periodical constituents, each one often described as a sum of sinusoidal components with a fundamental and some harmonics. In testing these methods, the paper confirms the hypothesis that the evolution of the electrical power (instantaneous and active) has a predominantly deterministic character. Two main signal analysis methods were used, with good, comparable results: the fast Fourier transform of short and long signal sequences (for the frequency domain) and the curve fitting estimation (in the time domain). The determination of the amplitude, frequency and phase at origin of time for each of these components helps to describe the condition (normal or abnormal) of the machine parts. Several achievements confirm the viability of this study: a characterization of a flat driving belt condition and a beating power phenomenon generated by two rotary shafts inside the gearbox. For comparison purposes, the same signal analysis methods were applied to describe the evolution of the vibration signal and the instantaneous angular speed signal at the gearbox output spindle. Many similarities in behaviour among certain mechanical parts (including their electrical power, vibration and instantaneous angular speed) were highlighted. Full article