The environment hosts a diversity of microorganisms whose potential for biotechnological applications has not yet been exhausted. The quest of our study was to find isolates of
Pichia kudriavzevii from the environment that could be used as new biotechnological agents. Moreover, we aimed
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The environment hosts a diversity of microorganisms whose potential for biotechnological applications has not yet been exhausted. The quest of our study was to find isolates of
Pichia kudriavzevii from the environment that could be used as new biotechnological agents. Moreover, we aimed to explore the resource efficiency for microbial cultivation, in particular the efficiency of spent coffee grounds (SCG), an easily accessible waste coffee product with a high unutilized organic content. In this study,
Pichia kudriavzevii strain ZMUM_K002, a yeast strain isolated from a grape pomace compost, was investigated. Antifungal susceptibility, particularly fluconazole susceptibility, was assessed, and the strain’s biotechnological potential by comparing its ability to utilize low-cost carbon sources, including SCG, with a natural isolate of
Saccharomyces cerevisiae (strain ZMUM_K003) was assessed. The
P. kudriavzevii strain ZMUM_K002 exhibited higher fluconazole susceptibility and yielded more than 30% more biomass in optimized media formulations compared to
S. cerevisiae ZMUM_K003. These findings demonstrate that
P. kudriavzevii ZMUM_K002 has the potential for efficient biomass production in sustainable industrial biotechnology, particularly in processes requiring high biomass yields on alternative substrates.
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