Search Results (737)

Tung cake, a by-product of Vernicia fordii oil extraction, is an underutilized biomass residue rich in natural bioactive constituents and therefore shows potential for the development of sustainable protective formulations. In this study, tung cake-derived systems, including the aqueous extract, fermentation broth, and extract–ethanol mixtures with different ethanol volume fractions, were prepared and systematically evaluated as a unified protective system on two representative biological surfaces, namely rubberwood and fresh fruit. For rubberwood, the formulations were assessed in terms of uptake behavior, antifungal efficacy against Aspergillus niger, resistance to moisture swelling, and physicochemical characteristics using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). For fruit surfaces, preservation performance was evaluated through weight loss, decay rate, and color retention during storage. The results showed that formulation performance depended strongly on the preparation route and extract–ethanol mixture. In rubberwood, the 60–90% mixtures and the extract displayed showed better performance antifungal activity, with the 60%, 80%, and 90% mixtures reaching a control efficacy of 75.00% and the extract achieving 68.75%. The treatments also improved the dimensional stability of wood, and the water-saturated volumetric swelling rate decreased from 8.98% in the control to 5.63% in the extract-treated group. FTIR and XRD analyses indicated that the basic lignocellulosic chemical framework and cellulose-related diffraction features of rubberwood were largely retained after treatment, while treatment-dependent qualitative spectral and apparent diffraction differences were observed. SEM provided more direct evidence of surface-associated covering and reduced fungal attachment. A comparable protective tendency was also observed on fruit surfaces. In oranges, the 80% extract–ethanol mixture showed the most favorable preservation performance under the tested storage conditions, maintaining a decay rate of 0 throughout 10 days of storage, reducing weight loss to 17.76%, and preserving surface color more effectively than the control. Overall, the 80% ethanol mixture achieved the best balance between antimicrobial activity and barrier-related protection across both rubberwood and fruit surfaces. These findings demonstrate that tung cake waste can be converted into a bio-based protective system with potential mold-inhibiting and preservation functions across different biological substrates. Full article

In this study, 20% ethanol elution fraction(PC-20), 40% ethanol elution fraction(PC-40), 60% ethanol elution fraction(PC-60), and 80% ethanol elution fraction (PC-80)of Penthorum chinense polyphenols were obtained using AB-8 macroporous resin . Their in vitro bioactivities were compared to explore potential applications. A comprehensive phytochemical analysis identified 85 compounds, including 16 phenolic acids, 36 flavonoids, 24 hydrolyzed tannins, 7 anthocyanins, and 2 others. The results showed clear ethanol concentration-dependent variations in both compound composition and bioactivity. PC-20 had the highest levels of total polyphenols (418.45 mg/g), proanthocyanidins (84.95 mg/g), and tannins (10.61 mg/g), and also showed the best antioxidant capacity. PC-40 contained the most flavonoids (227.55 mg/g). PC-60 gave the strongest α-glucosidase inhibition (IC₅₀ = 0.79 µg/mL), while PC-20 was most effective against pancreatic lipase (IC₅₀ = 101.06 µg/mL) and also significantly activated the enzymes ADH and ALDH. Overall, PC-20 appears more suitable for applications aimed at antioxidant, anti-obesity, or liver-protective effects, whereas PC-60 is more promising for blood glucose control. This work provides a practical basis for selecting different ethanol fractions of P. chinense polyphenols according to specific functional needs. Full article

Background/Objectives: Sex differences in ethanol consumption have been reported in both humans and laboratory rodents, but the independent/dependent contributions of genetic and hormonal sex biasing mechanisms to these phenotypes have not yet been fully explored. Methods: To examine the contributions of sex-chromosome complement (SCC) and gonadal sex (GS) to ethanol consumption, we studied adolescent (28–32 days old) four core genotypes (FCG) mice on a C57BL/6J background, a model which allows for independent assortment of GS and SCC. A modified drinking-in-the-dark (DID) procedure was employed, in which mice were offered concurrent access to 20%, 10% and 0% ethanol (in water) in four daily 2 h sessions. Consumption at the level of individual bouts was recorded. Results: Overall ethanol intake differed substantially by group and was driven almost entirely by differences in consumption of the 20% ethanol solution; all groups preferred the 20% solution over the 10% and 0% solutions, but consumed similar amounts of the 10% and 0% solutions. Intake of the 20% ethanol solution followed the rank order XXM > XYM > XYF > XXF. This pattern reflects an interaction between SCC and GS, such that SCC effects were greatest in gonadal females (XY > XX), whereas GS effects were greatest in XX mice (gonadal males > gonadal females). Moreover, the magnitude of these effects varied both across and within drinking sessions. The behavioral microstructure of ethanol consumption (i.e., parameterization of within-session discriminable drinking bouts) support the validity of our three-bottle modification of the DID procedure as a model of binge-like consumption, because (1) the consumption rate of the 20% ethanol solution was ~80 g EtOH/kg/h within a bout (~12 s/bout, ~three bouts/session), (2) most of this ethanol consumption was completed in a single bout and (3) within-session ethanol consumption was greater earlier than later, indicating “front loading.” Conclusions: These results suggest that the effects of GS on binge-like ethanol consumption are observed in early adolescence and moderated by SCC in adolescent FCG mice, with GS effects most pronounced in XX mice and SCC effects evident mainly in gonadal females. Full article

Life expectancy in high-income countries is increasing, leading to a higher incidence of age-related neurodegenerative diseases. To address this urgent medical need, several molecular targets have been identified, including advanced glycation end products (AGEs) and tyrosinase. Given the well-established role of diet in counteracting degenerative processes, this study aimed to identify a potential food ingredient with combined anti-tyrosinase and anti-glycative properties. Acanthus mollis L. was selected based on its inclusion in the BelFrIt list and its known content of tyrosinase inhibitors, such as benzoxazinones and verbascoside. Extraction of A. mollis leaves was optimized using a design of experiments approach, comparing microwave- and ultrasound-assisted techniques. Optimal conditions were achieved using microwave-assisted extraction with ethanol 80%, 80 °C, one cycle, drug-to-solvent ratio of 10 mL/g. The optimized extract (at 5 mg/mL) inhibited tyrosinase activity by approximately 47%, increasing to 58% after chlorophyll removal. Moreover, the extract reduces AGEs formation in presence of methylglyoxal, with an activity at 1 mg/mL comparable with that of a well-known anti-glycative agent. A similar trend was observed in the reduction in methylglyoxal and glyoxal levels. Overall, these results support the potential of the optimized A. mollis extract as a functional food ingredient to counteract aging-related neurodegeneration. Full article

The aim of this study was to valorize by-products of blue poppy (Papaver somniferum), a widely used ingredient in the food industry. This study focused on the isolation of bioactive compounds from leaves, stems, roots, capsules and cold-pressed cake. All samples were subjected to conventional solid–liquid extraction (SLE) using ethanol–water solutions of varying concentrations (0, 20, 40, 60, 80 and 96%) as the extraction solvent. The obtained extracts were analyzed for total phenolic content (TP), hydroxycinnamic acids (HCA), flavonols (FL), total flavonoids (TF), condensed tannins (CT) and antioxidant activity. Furthermore, the extracts were subjected to untargeted LC-MS analysis for qualitative characterization. Ethanol concentration significantly influenced the extraction efficiency of bioactive compounds, with the optimal solvent varying depending on the plant part and the specific class of compounds analyzed. Based on TP and TF content, capsule extracts exhibited the highest polyphenol levels. HCAs were detected in extracts from leaves, capsules, and cold-pressed cake. In total, 62 compounds were identified, belonging to various biochemical classes, including organic acids, hydroxycinnamic acids, alkaloids, flavonoids, and fatty acids. Overall, the results indicate that poppy by-products are a valuable source of bioactive components, with promising applications across different industrial sectors. Full article

Peucephyllum schottii is an aromatic desert plant of the family Asteraceae, which has little scientific research regarding its phytochemical composition and pharmacological properties. This study aims to evaluate in detail the chemical composition and antioxidant, antibacterial, antidiabetic, and cytotoxic activities of the ethanol extract of P. schottii leaves. The chemical composition of the plant extract was analyzed by GC-MS. Total phenolic (TPC) and flavonoid (TFC) contents of the plant were calculated. An antioxidant assay of the plant material was performed by using the DPPH and ABTS tests. The antibacterial activities of P. schottii plant material against six pathogenic bacteria were studied by using the agar diffusion and MIC/MBC techniques. Colorimetric analysis, for its part, enabled the assessment of its antihyperglycemic activities (α-amylase and α-glucosidase) and its cytotoxic activities (in MCF-7 and HepG2 cells). The expressions of apoptotic proteins (caspases, Bcl2, and Bax), were analyzed by RT-PCR. The GC-MS findings showed the presence of complex phytoconstituents of P. schottii in the form of linoleic acid (19.48%), hexadecanoic acid (15.01%), and vitamin E (12.15%). There is high TPC (118.18 mg of GAE/g) and TFC (75.56 mg of QE/g) in P. schottii plant material. The plant showed significant antioxidant (≈105 μg/mL IC₅₀ in DPPH and ≈80 μg/mL IC₅₀ in ABTS) and broad-spectrum antibacterial activities, mostly against E. coli (MIC = 4.68 μg/mL), as well as antihyperglycemic activities against α-amylase (IC₅₀ = 334 μg/mL) and α-glucosidase (IC₅₀ = 196 μg/mL) enzymes. The plant material showed cytotoxic effects in MCF-7 and HepG2 cells in a concentration-dependent manner (IC₅₀ = 78 ± 1.13 μg/mL and 68.23 ± 2.41 μg/mL, respectively). These findings point to P. schottii leaf extract’s potential as a natural antioxidant, antibacterial, antidiabetic, and chemopreventive agent. Full article

The marine benthic invertebrate Urechis unicinctus exhibits extraordinary tolerance to hypoxic environments, making its coelomic fluid a unique and promising biological source for discovering novel stress-adapting macromolecules. Polysaccharides derived from the coelomic fluid of U. unicinctus were systematically extracted, fractionated, and characterized to investigate their structural features and associated biological activities. Gradient ethanol precipitation (30–80%) combined with DEAE-52 ion exchange chromatography yielded twelve fractions with distinct physicochemical properties. Significant variations were observed in molecular weight (10³–10⁵ Da), sulfate content (3.77–24.26%), and monosaccharide composition. High-ethanol fractions, particularly U68P and U18P (extracted at 60 °C and 100 °C, respectively, and both precipitated with 80% ethanol), were enriched in low-molecular-weight, highly sulfated heteropolysaccharides composed of galactose, fucose, glucosamine, and ribose. These fractions exhibited superior antioxidant activities, including strong scavenging effects against DPPH, ABTS, and hydroxyl radicals. Moreover, they demonstrated pronounced neuroprotective effects in the oxygen–glucose deprivation/reoxygenation (OGD/R) model using SH-SY5Y cells, significantly improving cell viability. Structure–activity relationship analysis revealed that reduced molecular weight, increased sulfation degree, and more diverse monosaccharide composition (e.g., more diverse monosaccharide composition) synergistically contribute to improved bioactivity by facilitating cellular uptake and exposing functional groups. In contrast, high-molecular-weight homoglucan fractions showed relatively weak effects. Overall, this study identifies U. unicinctus coelomic fluid as a promising source of bioactive polysaccharides and provides a theoretical basis for the development of marine-derived anti-hypoxic and antioxidant agents. Full article

The increasing generation of agri-food waste represents a significant environmental challenge, but also a valuable source of bioactive compounds with potential industrial applications. In this study, selected minimally processed agri-food wastes from the food processing industry were evaluated as potential sources of bioactive compounds and antioxidants. Seven types of agri-food waste were investigated: green bean cutting waste, yellow bean cutting waste, sweet corn waste from the air selector, edamame pods, pepper seed by-products, potato peels, and potato waste from the air selector. Solid–liquid extraction was performed using ethanol at different concentrations (20, 40, 60, 80, and 96%, w/w) as a green solvent. Total polyphenol content (TPC) and antioxidant activity (DPPH, FRAP, and ABTS assays) were determined. The results demonstrated significant differences among the investigated raw materials, with the highest antioxidant activity observed in the potato peel extracts. Specifically, the strongest activity was recorded using 40% ethanol, yielding values of 3.9596 mg TE/g DW for DPPH and 11.4555 mg TE/g DW for ABTS assays. In contrast, the highest FRAP value (2.3970 mg Fe²⁺/g DW) was obtained with 60% ethanol. The highest TPC was detected in pepper seed by-products, reaching 6.7829 mg GAE/g DW when extracted with 20% ethanol. Furthermore, selected extracts were subjected to LC-MS analysis to obtain a preliminary characterization of their chemical profiles. Untargeted LC-MS analysis identified 115 metabolites belonging to different chemical classes, highlighting agri-food waste as a rich source of bioactive compounds, particularly flavonoids and phenolic acids. These findings demonstrate agri-food wastes as sustainable sources of bioactive compounds and support their valorization within circular economy and green processing frameworks. Full article

Background/Objectives: Curcuma longa is widely recognized for its antioxidant, antimicrobial, and wound-related biological effects. The present study aimed to compare two extracts prepared from organic turmeric powder (Curcuma longa), using distilled water (CUR-H₂O) and 96% ethanol (CUR-EtOH), in terms of extraction yield, phytochemical profile, antimicrobial activity, and in vitro biological behavior relevant to future oral-health-oriented applications. Methods: The extracts were prepared by maceration followed by ultrasound-assisted processing, concentration, and lyophilization. Their antioxidant potential (AOP) was evaluated by DPPH assay, total phenolic content (TPC) by the Folin–Ciocalteu method, and targeted polyphenolic profile by UHPLC-MS. Antimicrobial activity was assessed by broth microdilution against Streptococcus mutans, Streptococcus oralis, and Candida albicans. In vitro biological activity was investigated on HaCaT keratinocytes. Results: CUR-EtOH extract showed a higher extraction yield than CUR-H₂O (5.13% vs. 2.01%), higher AOP (69.54 ± 0.49% vs. 53.35 ± 0.30%), and a higher TPC (163.87 ± 0.32 vs. 78.05 ± 0.28 mg GAE/g dry extract). Consistent with these TPC results, UHPLC-MS revealed a richer targeted polyphenolic profile in CUR-EtOH extract, particularly in terms of p-coumaric and ferulic acid derivatives. CUR-EtOH extract was more active against the tested oral streptococci, especially S. mutans (MIC 10 µL vs. 60 µL for CUR-H₂O), whereas CUR-H₂O extract showed a slightly better antifungal effect against C. albicans (MIC 60 µL vs. 80 µL). In HaCaT cells, CUR-H₂O extract exhibited the more favorable compatibility profile, while CUR-EtOH extract showed stronger cytotoxicity, despite promoting faster wound-gap closure at 10 µg/mL. Conclusions: The extraction solvent strongly influenced both the chemical profile and biological behavior of the turmeric-powder-derived extracts. These findings suggest that solvent selection may be used to tailor the balance between antimicrobial efficacy and epithelial compatibility in future turmeric-powder-derived preparations intended for oral-health-oriented applications. Full article

Alzheimer’s disease (AD) is a common neurodegenerative disorder linked to cholinergic dysfunction, with butyrylcholinesterase (BChE) being a key therapeutic target for moderate–severe AD. Cortex Mori Radicis, a traditional Chinese medicinal herb, is rich in Diels–Alder adducts with potential neuroprotective effects; here, eighteen Diels–Alder adducts (four new: morusalbanol B–E, 1–4) were isolated and identified from its 80% ethanol extract. Their cholinesterase inhibitory activities were assessed via Ellman’s method, with enzyme kinetics and molecular docking performed for active compounds. Most compounds showed selective BChE inhibition, with kuwanon X (14) being the most potent (IC₅₀ = 2.3 μM). morusalbanol B (1), cathayanon A (8), and kuwanon G (12) acted as noncompetitive inhibitors, while Morusalbanol C (2) and kuwanon X (14) were mixed competitive inhibitors. Molecular docking suggested that potent inhibitors occupied the BChE active pocket via hydrogen bonds, π-π stacking, and hydrophobic interactions with Trp82, His438, and Phe329. MD simulations and MM-GBSA binding free energy analysis further verified that all three representative complexes (1, 8, and 14) achieved favorable thermodynamic and structural stability, with binding driven primarily by van der Waals forces. Residue decomposition revealed that Trp82 and Phe329 served as core binding hotspots for all tested inhibitors. Structure–activity analysis indicated that a cis-trans methylcyclohexene configuration, shorter aliphatic ester chains, and more prenyl groups enhanced BChE inhibition. This study provides new lead compounds and a systematic molecular mechanism basis for developing novel anti-AD BChE inhibitors from natural products. Full article

Olive pomace (OP) has been widely reported as a rich source of phenolic compounds with potential application as food additives with health-promoting properties. The aim of this work was to evaluate pressurized liquid extraction (PLE) as a strategy to obtain antioxidant and antimicrobial extracts from OP. Extractions were carried out in laboratory-scale equipment following a combined static/dynamic procedure. The extraction temperature (100, 120 and 140 °C) and the composition of solvent (50, 75 and 100% ethanol in water) were studied as independent variables of the process using a Face Centered Central Composite Design (α = 1). According to the fitted quadratic model (p < 0.05), the maximum Total Phenol Content (TPC) and Trolox Equivalent Antioxidant Capacity (TEAC) values were obtained at 120 °C using ethanol concentrations between 60 and 80%. Chemical characterization by RP/HPLC-Q-TOF MS/MS allowed the tentative identification of 37 compounds, with quinic acid being the most abundant compound under all extraction conditions, followed by elenolic acid, dimethyl-hydroxy-verbascoside, maslinic acid, hydroxy-verbascoside and oleuropein aglycone. Other secoiridoids, secoridoid derivatives, flavonoids, simple phenols and triterpenic acids were also identified. The extract obtained at 120 °C with 75% ethanol was able to protect purified sunflower oil in an accelerated oxidative stability test (Rancimat), increasing its induction period by 2.4-fold when added at 1000 mg/kg. This extract also exhibited antimicrobial activity against S. aureus, B. cereus, S. enterica and S. sonnei with a Minimum Inhibitory Concentration (MIC) of 3.6 mg/mL. These results highlight the potential of PLE olive pomace extracts as natural preservatives for food applications. Full article

This study aimed to develop and characterize biodegradable films based on xylan and polyvinyl alcohol (PVA), incorporated with ethanolic extract of Zanthoxylum rhoifolium Lam., for application in active food packaging. A Box–Behnken experimental design was employed to optimize the concentrations of xylan, PVA, and glycerol, evaluating the mechanical properties of the films. The results indicated that glycerol reduced tensile strength and increased elongation at break, while higher concentrations of xylan and PVA promoted an increase in mechanical strength. FTIR and XRD analyses reveal structural change without the formation of new chemical structures. The Z. rhoifolium extract exhibited antibacterial activity with a minimum inhibitory concentration of 31.5 µg/mL for Klebsiella pneumoniae, increasing to 1000 µg/mL for Enterococcus faecium, Enterobacter cloacae, and Escherichia coli. In addition, hemolytic activity exceeded 80% at concentrations ≥ 1000 µg/mL, whereas concentrations up to 500 µg/mL can be considered safe, as hemolysis remained below 40%. The films incorporated with the extract showed antimicrobial activity, with emphasis on the formulation containing 60 mg of extract, which exhibited inhibition zones of up to 16 mm against Escherichia coli and 12 mm against Enterococcus faecalis. Thus, the results highlight the potential of functionalized xylan/PVA films as sustainable materials for the development of active packaging. Full article

The valorization of agri-food by-products aligns with circular economy principles and offers sustainable sources of bioactive compounds. This study investigated the peels of the purple-fleshed Solanum tuberosum L. cv. Vitelotte Noire (VN), cultivated in Sardinia, as a potential resource for nutraceutical antioxidants. Extracts were obtained using solvents of different polarities (water, 80% and 96% ethanol) and characterized. Phytochemical screening revealed high concentrations of total phenolics, flavonoids, and anthocyanins, with the 96% ethanolic extract showing superior anthocyanin content. Antioxidant capacity, assessed via ORAC-PYR, TEAC-ABTS, and DPPH assays, was highest in the alcoholic extracts. Furthermore, all extracts showed protective effects in an in vitro model of AAPH-induced oxidative DNA damage, as indicated by the preservation of plasmid supercoiling. Untargeted LC-QTOF-MS analysis detailed a rich metabolomic profile, including organic acids, amino acids, and vitamins. The findings confirm VN peel as a potent, sustainable source of antioxidants, supporting its valorization for developing high-added-value nutraceutical and functional food ingredients, while reducing waste disposal costs and environmental impact. Full article

This work presents the design and evaluation of cerium and zinc oxide-incorporated indium oxide (Ce/ZnO-In₂O₃) nanocube composites synthesized via a hydrothermal process for advanced ethanol gas sensing. The incorporation of Ce and ZnO effectively modified the surface chemistry and electronic structure of In₂O₃ without causing significant morphological degradation. Compared with pristine In₂O₃, the Ce/ZnO-In₂O₃ sensor exhibited a significantly enhanced response of 33.2 toward 100 ppm ethanol at 300 °C, corresponding to an 8.7-fold improvement, along with a low detection limit of 0.8 ppm. In addition, the composite sensor demonstrated stable and reversible sensing behavior, excellent repeatability over 100 cycles, and long-term operational stability. Notably, improved humidity tolerance was achieved, with approximately 77% of the initial response retained at 80% relative humidity. The enhanced sensing performance is attributed to the combined effects of heterojunction formation between ZnO and In₂O₃ and Ce-induced lattice distortion, which promote oxygen adsorption and facilitate charge transfer during gas reactions. Principal component analysis (PCA) further confirmed the improved discrimination of ethanol against interfering gases. These results underscore the synergistic effects of Ce and ZnO incorporation in tailoring electronic structures and surface chemistry, thereby emphasizing the potential of this strategy for reliable ethanol detection in environmental and industrial applications. Full article

Since chemical anti-allergic compounds have adverse effects, many investigators pay attention to relatively safe natural products. Twenty-four newly crossbred rosebuds were extracted with 80% ethanol and analyzed for polyphenols, flavonoids, tannins, proanthocyanidins, and pyrogallol (1,2,3-benzenetriol). The extracts’ antioxidative, anti-allergic, and anti-inflammatory activities were assessed in vitro and in vivo. Among candidates, Lover Shy, Pretty Velvet, Ice Wing, Red Perfume, Onnuri, Jaemina Red, and Hanggina were found to possess high concentrations of antioxidant components and antioxidative activity. By comparison, Pretty Velvet, Red Perfume, Jaemina Red, Hanggina, Onnuri, and Ice Wing were highly effective in anti-allergic and anti-inflammatory activities in vitro, in parallel with their concentrations of pyrogallol. Their anti-allergic effects were confirmed in mice: The six extracts protected against Compound 48/80-induced mortality and scratching behaviors in a dose-dependent manner. The allergen-induced increases in serum IgE and histamine, as well as inflammatory cytokines, tumor-necrosis factor-α, and interleukin-1β, were remarkably attenuated following treatment with the rosebud extracts. These findings suggest that the extracts and active ingredients from cross-bred rosebuds exert anti-allergic and anti-inflammatory activities through their high levels of pyrogallol and antioxidants, and that they could be promising candidates to overcome allergic responses such as atopic dermatitis. Full article