Search Results (69)

Anthocyanins, crucial water-soluble pigments in plants, determine coloration in floral and fruit tissues, while fulfilling essential physiological roles in terms of plant growth, development, and stress adaptation. The biosynthesis of anthocyanins is transcriptionally regulated by WRKY factors, one of the largest plant-specific transcription factor families. Euscaphis japonica is an East Asian species, prized for its exceptionally persistent butterfly-shaped fruits that undergo pericarp dehiscence, overturning, and a color transition to scarlet red. This species represents an ideal system for studying anthocyanin regulation. However, the mechanisms by which WRKY transcription factors orchestrate anthocyanin accumulation during this process remain unknown. In this study, we identified 87 WRKY genes (EjaWRKYs) from the E. japonica genome. Phylogenetic analysis was used to classify these genes into three primary groups, with five subgroups, revealing conserved gene structures and motif compositions, supported by collinearity and comparative synteny analyses. Crucially, ten EjaWRKYs exhibited peak expression during the mature fruit stages, showing positive correlations with key anthocyanin biosynthesis genes. Functional validation through the use of transient transactivation assays in Nicotiana benthamiana confirmed that the five selected EjaWRKYs bind W-box elements and strongly activate reporter gene expression. Our results reveal EjaWRKYs’ regulation of anthocyanin accumulation in E. japonica fruit, provide the first comprehensive WRKY family characterization of this species, and establish a foundation for manipulating ornamental traits in horticultural breeding. Full article

This study proposes a novel control framework for semi-active seat suspensions, specifically targeting motion sickness mitigation through precision suppression of vertical vibrations within the 0.1–0.5 Hz frequency range. Firstly, a fractional-order band-stop filter in conjunction with a linear quadratic regulator (LQR) controller under frequency-domain sensitivity constraints (0.1–0.5 Hz) is proposed to achieve frequency-selective vibration attenuation. Secondly, the multi-objective butterfly optimization algorithm (MOBOA) is adopted to optimize the LQR controller’s weighting matrices (Q, R) by balancing conflicting requirements in terms of human body displacement limits, acceleration thresholds, and suspension travel. Finally, experimental validation under concrete pavement excitation and random road profiles demonstrates significant advantages over conventional LQR, i.e., a 41.04% reduction in vertical vibration amplitude and a 55.95% suppression of acceleration peaks within the target frequency band. The combined enhancements offer dual benefits of enhancing ride comfort and motion sickness mitigation in real-world driving scenarios. Full article

Studies on the influence of latitudinal and elevation gradient on species diversity, richness, and evenness are significant. The present study attempts to document the elevational distribution of nymphalidae butterflies in the surrounding regions outside the jurisdiction of the protected areas of the Eastern Himalayas. Determination of diversity, evenness, richness, dominance, and Gini’s coefficient was undertaken across six elevational belts (≤1500 masl, 1501–2000 masl, 2001–2500 masl, 2501–3000 masl, 3001–3500 masl, ≥3501 masl). The highest values of diversity (H’ = 6.34) and species richness (R₁ = 2.497; R₂ = 12.01) were represented by a mid-elevation peak at 2501–3000 masl. However, evenness values (E = 1.424; E_BG = 0.951; Eq = 0.987) were greatest at ≥3501 masl. Topographical uniqueness coupled with the formation of cloud cover at higher elevation may provide a habitat sustaining montane forest specialist species. Such differences in elevation could probably influence the diversity and distribution of nymphalidae, with immense ecological significance. Full article

Analyzing habitat conditions and mapping habitat structures are crucial for monitoring ecosystems and implementing effective conservation measures, especially in the context of declining open grassland ecosystems in Europe. The marsh fritillary (Euphydryas aurinia), an endangered butterfly species, depends heavily on specific habitat conditions found in these grasslands, making it vulnerable to environmental changes. To address this, we conducted a comprehensive habitat suitability analysis within the Hainich National Park in Thuringia, Germany, leveraging very high-resolution (VHR) airborne, red-green-blue (RGB), and color-infrared (CIR) remote sensing data and deep learning techniques. We generated habitat suitability models (HSM) to gain insights into the spatial factors influencing the occurrence of E. aurinia and to predict potential habitat suitability for the whole study site. Through a deep learning classification technique, we conducted biotope mapping and generated fine-scale spatial variables to model habitat suitability. By employing various modeling techniques, including Generalized Additive Models (GAM), Generalized Linear Models (GLM), and Random Forest (RF), we assessed the influence of different modeling parameters and pseudo-absence (PA) data generation on model performance. The biotope mapping achieved an overall accuracy of 81.8%, while the subsequent HSMs yielded accuracies ranging from 0.69 to 0.75, with RF showing slightly better performance. The models agree that homogeneous grasslands, paths, hedges, and areas with dense bush encroachment are unsuitable habitats, but they differ in their identification of high-suitability areas. Shrub proximity and density were identified as important factors influencing the occurrence of E. aurinia. Our findings underscore the critical role of human intervention in preserving habitat suitability, particularly in mitigating the adverse effects of natural succession dominated by shrubs and trees. Furthermore, our approach demonstrates the potential of VHR remote sensing data in mapping small-scale butterfly habitats, offering applicability to habitat mapping for various other species. Full article

Flavonoids have multiple functions, including host-plant defense against attacks from herbivorous insects. This manuscript reviewed and analyzed the scientific literature to test the hypothesis that flavonoids can be utilized to manage pests without causing significant harm to beneficials. The methodology involved using recognized literature databases, e.g., Web of Science, Scopus, and CAB Abstracts, via the USDA-ARS, National Agricultural Library, DigiTop literature retrieval system. Data were compiled in tables and subjected to statistical analysis, when appropriate. Flavonoids were generally harmful to true bugs and true flies but harmless to honey bees. Flavonoid glycosides showed a tendency to harm true bugs (Heteroptera) and true flies (Diptera). Flavonoid glycosides were harmless to sawflies. Flavonoids and flavonoid glycosides produced a mixture of harmful and harmless outcomes to herbivorous beetles, depending on the species. Flavonoid glycosides were harmless to butterflies. In conclusion, specific flavonoids could function as feeding stimulants or deterrents, oviposition stimulants or deterrents, chemical protectants from pesticides, mating attractants, less-toxic insecticides, and other functions. Flavonoids could manage some insect pests without causing significant harm to beneficials (e.g., honey bees). Flavonoid-based insecticides could serve as environmentally benign alternatives to broad-spectrum insecticides against some pests, but field testing is necessary. Full article

Hibernation is an energy-saving and adaptive strategy adopted by a diverse array of animals, rarely including warm-climate species, to survive in the harsh winter environment. Here, we collected large-intestinal microbial samples from two species of warm-climate lizards, one (the Reeves’ butterfly lizard Leiolepis reevesii) hibernating in the winter months and one (the many-lined sun skink Eutropis multifasciata) not doing so, in summer and winter to analyze and compare their microbiota using 16S rRNA gene amplicon sequencing technology. Gut microbiota were seasonally variable in L. reevesii but not in E. multifasciata. The decreased Firmicutes/Bacteroidetes ratio and increased relative abundance of Verrucomicrobia in hibernating butterfly lizards in a state of long-term fasting should help them live through the winter months, as bacteria of the phyla Bacteroidetes and Verrucomicrobia can use host-derived mucin glycans in the absence of dietary substrates. Facultative plant feeding by omnivorous butterfly lizards resulted in a significant increase in the relative abundance of bacteria of the phylum Firmicutes (e.g., Lachnospiraceae) with the ability to degrade plant fibers. This study not only validates the role of gut microbiota in dietary adaptation in lizards but also shows that gut microbial communities are seasonally variable in warm-climate lizards hibernating in the winter months. Full article

The growing interest in safeguarding agroecosystem biodiversity has led to interest in studying ecological interactions among the various organisms present within the agroecosystem. Indeed, mutualisms between weeds and pollinators are of crucial importance as they influence the respective survival dynamics. In this review, the mutualistic role of flower visitors and the possible (often predominant) abiotic alternatives to insect pollination (self- and wind-pollination) are investigated. Mutualistic relations are discussed in terms of reward (pollen and/or nectar) and attractiveness (color, shape, scent, nectar quality and quantity), analyzing whether and to what extent typical weeds are linked to pollinators by rigid (specialization) or flexible (generalization) mutualistic relations. The entomofauna involved is composed mainly of solitary and social bees, bumblebees, Diptera, and Lepidoptera. While some of these pollinators are polylectic, others are oligolectic, depending on the shape of their mouthparts, which can be suited to explore the flower corollas as function of their depths. Consequently, the persistence dynamics of weed species show more successful survival in plants that are basically (occasional insect pollination) or totally (self and/or wind pollination) unspecialized in mutualistic relations. However, even weed species with typical abiotic pollination are at times visited during periods such as late summer, in which plants with more abundant rewards are insufficiently present or completely absent. Many typically insect-pollinated weeds can represent a valid indicator of the ecological sustainability of crop management techniques, as their survival dynamics are closely dependent on the biodiversity of the surrounding entomofauna. In particular, the presence of plant communities of species pollinated above all by butterflies (e.g., several Caryophyllaceae) gives evidence to the ecological compatibility of the previous agronomic management, in the sense that butterflies require certain weed species for oviposition and subsequent larva rearing and, therefore, provide further evidence of plant biodiversity in the environment. Full article

Worldwide, large-scale biodiversity monitoring schemes are developing and involve many non-specialist volunteers. If the opening of schemes to non-specialists allows for the gathering of huge amounts of data, their quality represents a controversial issue. In the framework of the French Garden Butterfly Observatory (FGBO), we studied non-specialist volunteer identification errors based on identifications provided during a one-shot experiment. With 3492 butterfly pictures sent by 554 non-specialist volunteers, we directly measured identification errors and misidentification rates for each butterfly species or species group targeted by the FGBO. The results showed that when non-specialist volunteers identified butterflies at the species level, identification errors (i.e., the misidentification rate) reached 20.9%. It was only 5.0% when FGBO species groups were used. This study provides novel insights into the trade-off between data quantity and quality provided by non-specialist volunteers and shows that if protocols, research questions and identification levels are adapted, participatory monitoring schemes relying on non-specialists represent a powerful and reliable tool to study common species at a large scale and on a long-term basis. Full article

Parnassius butterflies have significantly advanced our understanding of biogeography, insect–plant interactions, and other fields of ecology and evolutionary biology. However, to date, little is known about the gene expression patterns related to the high-altitude adaptation of Parnassius species. In this study, we obtained high-throughput RNA-seq data of 48 adult Parnassius individuals covering 10 species from 12 localities in China, and deciphered their interspecific and intraspecific expression patterns based on comparative transcriptomic analyses. Though divergent transcriptional patterns among species and populations at different altitudes were found, a series of pathways related to genetic information processing (i.e., recombination, repair, transcription, RNA processing, and ribosome biogenesis), energy metabolism (i.e., oxidative phosphorylation, thermogenesis, and the citrate cycle), and cellular homeostasis were commonly enriched, reflecting similar strategies to cope with the high-altitude environments by activating energy metabolism, enhancing immune defense, and concurrently inhibiting cell growth and development. These findings deepen our understanding about the molecular mechanisms of adaptative evolution to extreme environments, and provide us with some theoretical criteria for the biodiversity conservation of alpine insects. Full article

This manuscript describes the synthesis of green long afterglow nanophosphors SrAl₂O₄:Eu²⁺, Nd³⁺ using the combustion process. The study encompassed the photoluminescence behavior, elemental composition, chemical valence, morphology, and phase purity of SrAl₂O₄:Eu²⁺, Nd³⁺ nanoparticles. The results demonstrate that after introducing Eu²⁺ into the matrix lattice, it exhibits an emission band centered at 508 nm when excited by 365 nm ultraviolet light, which is induced by the 4f⁶5d¹→4f⁷ transition of Eu²⁺ ions. The optimal doping concentrations of Eu²⁺ and Nd³⁺ were determined to be 2% and 1%, respectively. Based on X-ray diffraction (XRD) analysis, we have found that the physical phase was not altered by the doping of Eu²⁺ and Nd³⁺. Then, we analyzed and compared the quantum yield, fluorescence lifetime, and afterglow decay time of the samples; the co-doped ion Nd³⁺ itself does not emit light, but it can serve as an electron trap center to collect a portion of the electrons produced by the excitation of Eu²⁺, which gradually returns to the ground state after the excitation stops, generating an afterglow luminescence of about 15 s. The quantum yields of SrAl₂O₄:Eu²⁺ and SrAl₂O₄:Eu²⁺, Nd³⁺ phosphors were 41.59% and 10.10% and the fluorescence lifetimes were 404 ns and 76 ns, respectively. In addition, the E_g value of 4.98 eV was determined based on the diffuse reflectance spectra of the material, which closely matches the calculated bandgap value of SrAl₂O₄. The material can be combined with polyacrylic acid to create optical anti-counterfeiting ink, and the butterfly and ladybug patterns were effectively printed through screen printing; this demonstrates the potential use of phosphor in the realm of anti-counterfeiting printing. Full article

The diversification of the periodic ultrastructure of wing scales plays a crucial role in regulating the functional properties of butterfly wings, contributing to their ecological adaptation. This study addresses the structural regulation of mid-infrared radiation (MIR) in wing scales, a property associated with cooling in thermoregulation and pheromone release during courtship. Using Danainae (Papilionoidea: Nymphalidae) as the model group, the study confirms the high morphological diversity of butterfly wing scales in a single individual with quantitative observations under scanning and transmission electron microscopy. It was found that this diversity shapes the heterogeneity of the wing emissivity through heating experiments, virtual simulations, and correlation tests. Summarizing the effects of each component on emissivity, it was demonstrated that the increase in scale emissivity is due to the increase in its internal surface area and thickness. Additionally, it was demonstrated that, as the structural parameter positively correlates with emissivity increases, the area of scent patches, a high emissivity region where males emit pheromones, decreases significantly, whereas the size of scales on the scent patch increases significantly. A further study of 99 butterfly species from several families shows that as the range of butterfly species moves from low to high latitudes, which generally corresponds to a decrease in habitat temperature, the efficiency of infrared radiation in the wing scales decreases, i.e., the wing radiates less efficiently for cooling and less heat is dissipated. This phenomenon is also shaped by variations in the overall structure of the scales. The study provides a reference for understanding functional adaptation in butterflies. Full article

Hodgkin lymphomas are radiosensitive and curable tumors that often involve the mediastinum. However, the application of radiation therapy to the mediastinum is associated with late effects including cardiac and pulmonary toxicities and secondary cancers. The adoption of conformal IMRT and deep inspiration breath- hold (DIBH) can reduce the dose to healthy normal tissues (lungs, heart and breast). We compared the dosimetry of organs at risk (OARs) using different IMRT techniques for two breathing conditions, i.e., deep inspiration breath hold (DIBH) and free breathing. Twenty-three patients with early-stage mediastinal Hodgkin lymphomas were accrued in the prospective study. The patients were given treatment plans which utilized full arc volumetric modulated arc therapy (F-VMAT), Butterfly VMAT (B-VMAT), and fixed field IMRT (FF-IMRT) techniques for both DIBH and free breathing methods, respectively. All the plans were optimized to deliver 95% of the prescription dose which was 25.2 Gy to 95% of the PTV volume. The mean dose and standard error of the mean for each OAR, conformity index (CI), and homogeneity index (HI) for the target using the three planning techniques were calculated and compared using Student’s t-test for parametric data and Wilcoxon signed-rank test for non-parametric data. The HI and CI of the target was not compromised using the DIBH technique for mediastinal lymphomas. The mean values of CI and HI for both DIBH and FB were comparable. The mean heart doses were reduced by 2.1 Gy, 2.54 Gy, and 2.38 Gy in DIBH compared to FB for the F-VMAT, B-VMAT, and IMRT techniques, respectively. There was a significant reduction in V5Gy, V10Gy, and V15Gy to the heart (p < 0.005) with DIBH. DIBH reduced the mean dose to the total lung by 1.19 Gy, 1.47 Gy, and 1.3 Gy, respectively. Among the 14 female patients, there was a reduction in the mean right breast dose with DIBH compared to FB (4.47 Gy vs. 3.63 Gy, p = 0.004). DIBH results in lower heart, lung, and breast doses than free breathing in mediastinal Hodgkin Lymphoma. Among the different IMRT techniques, FF-IMRT, B-VMAT, and F-VMAT showed similar PTV coverage, with similar conformity and homogeneity indices. However, the time taken for FF-IMRT was much longer than for the F-VMAT and B-VMAT techniques for both breathing methods. B-VMAT and F-VMAT emerged as the optimal planning techniques able to achieve the best target coverage and lower doses to the OARs, with less time required to deliver the prescribed dose. Full article

The combination of animals and plants in zoological gardens provides the opportunity for conservation within the grounds. An example is pollination gardens that support free-moving butterflies and bees. Pollination gardens and other areas with plants also support a wide range of other non-captive animals, including soil-associated invertebrates. This study targeted land snails, a species-rich group that links the brown decomposition food web and the plant-based web that is preyed upon by fireflies and birds, among others. A survey of 24 sites within the Oklahoma City Zoo and Botanical Garden revealed a diverse snail fauna of 23 species. The majority were native species but included 10 non-native species that are common regional urban species, likely introduced through the plant trade. No distributional pattern of snails was evident, probably because of the similar management scheme throughout the zoo. Snails and other observed soil-associated invertebrates (e.g., isopods, millipedes, and earthworms) support non-captive birds and other animals. Lightly managed areas with leaf litter and downed wood are especially conducive to snails and other soil-associated invertebrates and are nesting sites for bees. These communities can form conservation demonstration areas that can be the basis of educational programs, including outreach programs. Full article

Although elliptical tubes are stronger and more stable than circular tubes, few studies have fully considered the behavior of elliptical tubes under cyclic bending loads. This study experimentally investigated the response and failure of SUS304 stainless steel elliptical tubes with four different ratios of long and short axes (1.5, 2.0, 2.5, and 3.0) under cyclic bending along four different orientation angles (0°, 30°, 60°, and 90°). The wall thickness was 0.7 mm, and cyclic bending was applied until buckling failure occurred. The moment–curvature curves exhibited cyclic hardening, and stable loops were formed for all long–short axis ratios and orientation angles. Increasing the long–short axis ratio slightly decreased the peak bending moment while increasing the orientation angle increased the peak bending moment. For a given orientation angle, the curves relating the short-axis variation (i.e., change in length divided by the original length of the short axis) and curvature demonstrated symmetry, serrations, and a growth pattern as the number of cycles increased regardless of the long–short axis ratio. At long–short axis ratios of 2.0, 2.5, and 3.0, these curves even exhibited a butterfly-like trend. Increasing the long–short axis ratio increased the short-axis variation, while increasing the orientation angle decreased the short-axis variation. Regarding the curves relating the curvature and number of cycles required to initiate buckling, for each orientation angle, the four long–short axis ratios corresponded to four straight lines when plotted on double-logarithmic co-ordinates. Based on the experimental results, empirical equations are proposed to describe the above relationships. The empirical equations were applied to predicting experimental data and showed close agreement. Full article

Buried flexible pipes are allowed to deflect up to 2–5% of the pipe diameter, which can become problematic for the connected direct-bury, large-diameter butterfly valves. The complex behavior of the pipe–valve–soil system makes it difficult to predict the deflection of the pipe/valve system. In the absence of field/experimental studies, the application of finite element analysis (FEA) seems necessary to predict deflection and stresses and to avoid potential downtime associated with disruption of service. This paper described the FEA of a large-diameter pipe–valve system, with different backfills under gravity, overburden, and internal pressure loads. The effects of modeling different components of the system (e.g., flanges, bearing housing, gate disc, etc.) were described and investigated. The goal of this study was to provide insight into the design and installation of direct-bury pipe–valve systems and evaluate current installation methods in the absence of guidelines. In addition, the level of modeling details required for FEA to yield accurate results was discussed. Full article