Search Results (1,907)

Background: Parental knowledge and perceptions towards Functional Foods (FFs) play a critical role in shaping children’s dietary behaviors. This study aimed to investigate dietary habits, FFs knowledge and perceptions among Greek primary school children and their parents and to evaluate the feasibility of a one-month pilot asynchronous nutrition education program. Methods: A cross-sectional study included 374 children aged 9–11 years and 159 parents from urban (Thessaloniki) and rural (Lemnos) areas. Children completed questionnaires on dietary habits, FFs knowledge and Mediterranean Diet (MD) adherence (KIDMED score), while parents provided sociodemographic information, BMI, dietary habits, FFs knowledge and perceptions. A pilot asynchronous nutrition education intervention was delivered via pre-recorded videos on FFs, the MD, portion sizes and food label interpretation, with participation tracked and program evaluation conducted among parents. Data was analyzed using IBM SPSS Statistics (version 28). Descriptive statistics were calculated, group differences were assessed with t-tests and ANOVA and associations between variables were examined using chi-square tests and Pearson correlations (p < 0.06). Results: Children showed moderate MD adherence, frequent fast-food and soft drinks consumption and low FF knowledge, with a substantial gap between perceived and actual understanding. Parental FF knowledge was uneven, higher among normal-weight participants and largely limited to fortified products. Positive associations were found between children’s and parents’ diet quality and natural FF consumption, as well as between parental and child physical activity. The asynchronous intervention was positively rated; substantial attrition was observed across sessions and follow-up, which limited the ability to assess the intervention’s effects on behavioral change. Conclusions: This study highlights critical gaps in FFs knowledge among families and demonstrates that asynchronous, family-based nutrition education is feasible but challenged by engagement attrition. Targeted interventions are needed to clarify FF concepts and promote healthier family dietary behaviors. Full article

Background/Objectives: Approximately 1 in 10 community-dwelling older adults are affected by or at risk of malnutrition, and this prevalence increases to nearly 1 in 3 among those receiving home care or recently hospitalized, contributing to higher rates of frailty, falls, hospitalization, functional decline, and mortality. Many of these individuals depend on informal or family caregivers for nutritional care, including assistance with grocery shopping meal preparation, feeding, and monitoring dietary intake. Thus, informal caregivers play an increasingly central role in supporting dietary intake and maintaining nutritional status. This narrative review aims at assessing the relationship between informal caregiver involvement and malnutrition in community-dwelling older adults who are dependent for nutritional-related needs, summarizing evidence on caregiver’s role and caregiver-associated determinants of malnutrition, as well as on interventions that incorporate caregivers into nutrition care. We discuss factors associated with malnutrition in later life, with particular emphasis on caregiver knowledge, burden, interventions and outcomes. In addition, caregiver-inclusive models of care and tools, including nutrition education and guidelines/recommendations, medical nutrition therapy, and multidisciplinary care models will be addressed. Methods: A structured review of the literature was conducted (date of last search December 2025), searching multiple databases for pertinent articles. Following identification of eligible articles for inclusion, a narrative synthesis of evidence was completed. Results and Conclusions: Despite the high degree of heterogeneity in methodology, observational studies demonstrate that several caregiver attributes influence the nutritional status of care recipients, including caregiver’s own nutritional status, burden, knowledge and literacy, psychosocial, environmental and economic factors. Intervention studies show that caregiver-focused, -inclusive and -delivered interventions have a positive impact on several outcomes, including improved older care recipient dietary intakes, nutritional status and quality of life without impacting on caregiver burden. Thus, strengthening caregiver support and integrating caregivers into nutrition screening and intervention frameworks may represent a critical opportunity to reduce malnutrition risk and improve health outcomes among older adults. Still, significant gaps remain in caregiver-focused intervention research, particularly in diverse cultural and social contexts. Full article

Volatile fatty acids (VFAs), the primary end-products of microbial fermentation in the ruminant forestomach, supply approximately 70% of the host’s energy requirements and play a pivotal role in maintaining energy homeostasis. While the mechanisms governing ruminal VFA production, absorption, and metabolism are well-characterized in common ruminants like dairy and beef cattle, a systematic integration of these processes in yaks, an iconic species long-adapted to the extreme Qinghai–Tibet Plateau, remains incomplete. This review synthesizes current knowledge on the entire VFA pathway in the yak rumen, from production to tissue metabolism. We detail the critical roles of functional microbes, including fibrolytic bacteria and Prevotella, in VFA synthesis and how their activity is dynamically regulated by dietary composition and seasonal shifts. Building on the unique structural features of the yak rumen epithelium, the review analyzes VFA absorption mechanisms involving both passive diffusion and carrier-mediated transport. Furthermore, we systematically outline the metabolic fates and energy partitioning strategies of VFAs across the rumen epithelium, liver, and peripheral tissues. This synthesis aims to elucidate the highly efficient and adaptive physiological basis of VFA metabolism that underpins the yak’s exceptional ability to utilize energy under the low-energy conditions of the high-altitude environment. Ultimately, this work seeks to provide a theoretical foundation for understanding plateau-adapted energy efficiency and to inform precision nutritional strategies for ruminants in alpine regions. Full article

In the present study, 162 samples of KAMUT^® khorasan wheat (Triticum turgidum ssp. turanicum) harvested in North America during three cropping seasons (2010, 2011, and 2012) were analyzed to highlight direct and indirect associations with the main agrometeorological components via path analysis. Agronomic traits (yield), nutritional (test weight, protein, and starch content), and nutraceutical composition (dietary fibre, polyphenol, and flavonoid content) were examined. Path coefficient analysis showed that mean temperature (−0.377) and rainfall (−0.196) had high negative direct effects on yield. Among the qualitative traits, the content of free polyphenols (−0.568) and soluble dietary fibre (+0.393) was highly correlated with mean temperature, respectively, while the content of bound polyphenols was correlated with rainfall (+0.455). In addition, results allowed us to fill the existing knowledge gap, highlighting the direct and indirect effects of agroclimatic variables on yield, nutritional, and nutraceutical traits. Full article

Background/Objectives: The use of over-the-counter vitamin D₃ supplements has increased substantially in recent years. Compared with pharmaceuticals, dietary supplements are subject to less stringent regulatory oversight, raising concerns regarding labeling accuracy, consumer knowledge, and patient safety. This study aimed to assess public knowledge and preferences related to vitamin D₃ supplementation and to evaluate the content accuracy and short-term stability of commonly used products. Methods: A cross-sectional online survey containing 39 questions was conducted in Hungary between 1 May and 30 June 2024. Based on survey responses, the most frequently used vitamin D₃ supplements (five soft gel capsules and four tablets) were selected for laboratory analysis. Vitamin D₃ content was quantified using a validated high-performance liquid chromatography (HPLC) method with UV detection. Soft gel capsules were additionally exposed to natural daylight for one month to assess short-term photostability. Results: In total, 367 participants (mean age 31.0 ± 12.5 years) completed the survey, and only 3.5% answered correctly all knowledge-based questions. Six commonly reported supplement brands accounted for approximately 90% of responses. Measured vitamin D₃ content remained within the tolerance limit (−20% to +50%). Following sunlight exposure, three of four capsule products showed no substantial vitamin D₃ loss, while one exhibited a 14.7% decrease. Conclusions: Most analyzed vitamin D₃ supplements complied with labeled content claims, but substantial knowledge gaps were identified that may affect patient safety. The validated HPLC method supports pharmacovigilance-oriented quality monitoring of vitamin D₃ supplements and underscores the need for improved professional counseling. Full article

Background: Reducing dietary salt intake is a global public health priority. However, empirical evidence is needed to clarify whether higher levels of health literacy (HL) and eHealth literacy (eHL), together with the use of diversified information dissemination channels, are positively associated with better salt-related knowledge, attitudes, and practices (KAP). This study examined the indirect associations via eHL and the number of sources of salt-reduction information (NSSI) in the relationship between HL and salt-related KAP. Methods: A cross-sectional survey was conducted from 2022 to 2023 using multistage stratified random sampling among residents aged 15–69 in 22 counties/districts of Jiangxi Province, China. Data on sociodemographic characteristics, HL, eHL, NSSI, and salt-related KAP were collected through face-to-face household interviews using a standardized electronic questionnaire system. Spearman correlation analysis and multiple linear regression were applied to assess associations among HL, eHL, NSSI, and salt-related KAP. Path analysis was employed to evaluate the indirect associations between HL and salt-related KAP via eHL and NSSI. Results: A total of 5396 residents participated, of whom 51.50% were male. Participants aged 15–34, 35–54, and 55–69 years accounted for 13.10%, 42.96%, and 43.94% of the sample, respectively. After adjustment for covariates, individuals with adequate HL, adequate eHL, and a greater NSSI had significantly higher total salt-related KAP scores (p < 0.001). In the path analysis, the standardized direct association of HL with the total salt-related KAP was 0.229 (p < 0.001). The standardized indirect associations via NSSI and eHL were 0.089 (95% CI: 0.069 to 0.111, p < 0.001) and 0.057 (95% CI: 0.033 to 0.089, p < 0.001), respectively, accounting for 23.73% and 15.20% of the total association. Conclusions: High levels of HL and eHL, together with increased exposure to multiple salt-reduction information sources, are associated with improved salt-related KAP. Both eHL and NSSI partially explain the association between HL and salt-related KAP. Future salt-reduction interventions should integrate conventional health education with mobile health technologies to expand information dissemination channels and support sustained salt-reduction behaviors. Full article

The milpa (a traditional Mesoamerican agricultural system) constitutes a dynamic space with a great diversity of agro-food and sociocultural resources. The objective of this study was to evaluate the milpa’s contribution to food self-sufficiency and to document perceptions of its attributes and cultural significance in San Felipe Tejalápam, Oaxaca. Using a mixed-methods design that included food frequency questionnaires, 24 h dietary recalls (n = 50), and semi-structured interviews (n = 12), the percentage of adequacy (PA) and the symbolic perception of the milpa were analyzed. The results indicate that milpa foods are important for local nutrition, providing significantly higher percentages of adequacy (p ≤ 0.05) for carbohydrates (63.72%), fiber (69.72%), and iron (77.22%). However, the system proved insufficient in energy (42.35%), protein (32.38%), vitamin C (2.69%), vitamin E (0.17%), and potassium (11.14%) compared to external food sources. From a cultural perspective, the milpa was identified as a pillar of community identity. Participants highlighted its culinary properties, health benefits, and its role as a driver of economic and food self-sufficiency. It is concluded that the milpa not only satisfies key nutritional needs but also preserves ancestral knowledge and strengthens the social fabric of the population. Full article

Background: Sustainable dietary transitions are increasingly emphasized in public health policy; however, validated psychometric instruments assessing knowledge, attitudes, and practices (KAP) in Asian contexts remain limited. Objectives: This study aimed to develop and evaluate a KAP questionnaire on sustainable diets and examine sociodemographic variations and behavioral correlates among Taiwanese adults. Methods: A two-phase mixed-methods design was used, including expert validation and a cross-sectional online survey (n = 587). Construct validity was examined using exploratory factor analysis, and reliability was evaluated through internal consistency and test–retest assessments. Multivariable logistic regression models were fitted to identify factors associated with low adherence to a low-carbon diet. Model diagnostics included variance inflation factors (VIF), Hosmer–Lemeshow tests, Nagelkerke pseudo-R², and ROC analysis. Results: Content validity was high (S-CVI/Ave = 0.95–0.98). The attitude and practice domains demonstrated satisfactory internal consistency, whereas the knowledge domain showed comparatively lower reliability. In multivariable logistic regression analyses, higher attitude scores were independently associated with lower odds of low adherence to a low-carbon diet. Eating-out frequency and clear awareness of sustainability in Taiwan’s Dietary Guidelines were also significantly associated with adherence. The fully adjusted model demonstrated adequate calibration and excellent discrimination (AUC = 0.778). Conclusions: The instrument provides preliminary psychometric evidence supporting the assessment of sustainable diet-related KAP among Taiwanese adults. Attitudes appear more strongly associated with dietary practices than knowledge alone. The questionnaire may support future monitoring and research. Full article

Background: Childhood and adolescence represent a critical period for shaping long-term dietary habits, including whole grain consumption, which remains low despite well-documented health benefits. Objective: This cross-sectional study (November–December 2024) examined Slovenian adolescents’ attitudes toward whole-grain foods in the context of organized school meals. Methods: Participants aged 10–12 years and 14–19 years (N = 501; mean age 15.6 ± 2.6) completed an online questionnaire assessing knowledge, self-reported consumption frequency, preferences, motivational factors, and food neophobia using the translated Italian Child Food Neophobia Scale (ICFNS). Based on ICFNS scores, participants were classified as low (≤17), medium (18–24), or high (≥25) in food neophobia. Results: Older adolescents demonstrated better knowledge of whole-grain health benefits; however, greater knowledge was not associated with higher self-reported consumption. Food neophobia was strongly associated with lower consumption frequency and reduced willingness to try whole-grain foods, including whole-grain bread, oatmeal, buckwheat porridge and brown rice. Across all groups, taste was the most consistent motivator for trying whole-grain foods. Older adolescents prioritized health and appearance as key reasons for eating more whole grain foods. Conclusions: Findings suggest that improving taste, increasing exposure, and leveraging institutional settings such as schools, where availability, preparation, and social cues can be managed, may be effective in promoting whole-grain food consumption. Full article

Background: Nutritional psychoeducation is a core component of multidisciplinary treatment for anorexia nervosa, yet evidence on its association with changes in eating behavior beyond weight outcomes remains limited. Methods: This pre–post observational study included 45 patients with anorexia nervosa attending a Day Hospital program who participated in a structured, group-based nutritional psychoeducational intervention as part of standard multidisciplinary care. Nutritional knowledge, dietary rules, eating behaviors, food group exposure assessed by a Food Frequency Questionnaire (FFQ), and body mass index (BMI) were evaluated before and after the intervention. Pre–post changes were examined using paired statistical tests with Holm correction. Associations between changes in cognitive–nutritional variables and eating behavior were explored using correlations and multiple linear regression models. Results: Significant pre–post improvements were observed in nutritional knowledge and reductions in rigid dietary rules. Eating behavior showed specific changes, with increased exposure to carbohydrate- and fat-containing foods, as well as improved meal adequacy. BMI increased during the observation period, consistent with expected outcomes of Day Hospital treatment. Changes in nutrient-related knowledge were positively associated with changes in dietary fat exposure, independent of baseline BMI and changes in dietary rules, whereas no comparable association was observed for carbohydrate exposure. Conclusions: In this Day Hospital sample, participation in a group-based nutritional psychoeducational program within a multidisciplinary treatment context was associated with specific changes in eating behavior alongside cognitive–nutritional changes and weight gain. The observed association between nutrient-related knowledge and dietary fat exposure may suggest the relevance of assessing food-specific behaviors and cognitive–nutritional processes as complementary outcomes during treatment for anorexia nervosa. Full article

Oxidative stress (OS) is a central regulator of health and productivity in livestock, emerging from complex interactions between dietary inputs, microbiome composition, environmental stressors, and host metabolism. This narrative review synthesizes current knowledge on OS in cattle, pigs, sheep, and poultry, emphasizing mechanistic pathways, tissue-specific responses, and translational applications. We highlight the central role of redox–inflammatory signaling hubs, including nuclear factor kappa B (NF-κB), nuclear factor erythroid 2–related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1), and inflammasomes, as integrators of metabolic and immune stress. Microbiome–metabolome interactions modulate systemic oxidative responses, influencing liver, mammary gland, gastrointestinal tract, adipose tissue, and reproductive tissues. Oxidative stress-related biochemical and molecular alterations are captured by a range of biomarkers, such as malondialdehyde (MDA), Total Antioxidant Capacity (TOAC), gluthatione peroxidase (GPx), superoxide dismutase (SOD), paraoxonase-1 (PON1), cytokines, and gene expression profiles, measurable in blood, milk, saliva, and tissues. Integrating these markers enables precision diagnostics, early disease detection, and evidence-based nutritional interventions. Furthermore, computational modeling and spatial–socioeconomic perspectives offer novel approaches to translate molecular redox insights into practical livestock management strategies. By framing OS as a regulated, context-dependent process rather than a simple imbalance of reactive oxygen species, this review advances a conceptual, cross-species framework for understanding, monitoring, and mitigating oxidative stress in livestock. This integrative perspective provides a foundation for targeted antioxidant strategies and sustainable production practices, bridging molecular mechanisms with practical applications in animal health and productivity. Full article

Ultra-endurance running presents significant physiological demands, with adequate nutritional intake being critical for optimal preparation, performance, and recovery. However, athletes of this sport often consume an insufficient diet. Semi-structured interviews were conducted virtually with ten recreational ultra-endurance runners (age mean ± standard deviation 46 ± 12 years; eight male, two female), all with experience competing in ultra-endurance events, to explore their ultra-endurance experience, dietary intake, nutrition knowledge, and gastrointestinal symptom management. Interviews were transcribed verbatim and thematically analysed in accordance with the COM-B model and the Theoretical Domains Framework. Themes were defined under each of the three COM-B model components: Capability: (1) knowledge and skills, (2) the intention–behaviour challenge; Opportunity: (1) facilitators and barriers to optimal nutrition, (2) information sourcing and learning; and Motivation: (1) drivers of nutrition behaviours, (2) risk perceptions’ influence on fuelling strategies. Participants demonstrated strong psychological capability, that is, awareness of nutrition’s importance, yet limited behavioural regulation to translate this knowledge into practice. Nutrition strategies were largely self-directed, relying on peers and social media over professional support. Fear of gastrointestinal symptoms and time pressures further shaped dietary decisions. These findings emphasise the importance of evidence-based nutrition guidance to support performance and recovery while minimising gastrointestinal symptom risk. For practitioners and self-coached athletes, recommendations should reflect the practical realities and competing demands of ultra-endurance runners’ daily lives and be feasible within real-world settings. Full article

Thymus species play an important role in traditional medicine, food practices, and cultural heritage across the Balkan Peninsula, a region characterized by high floristic diversity and long-standing ethnobotanical traditions. This review provides a comprehensive synthesis of published ethnobotanical and ethnopharmacological data on the traditional uses of Thymus species in the Balkans. A systematic survey of literature published between 1900 and 2022 was conducted using major scientific databases and classical ethnobotanical sources, covering Albania, Bosnia and Herzegovina, Bulgaria, Croatia, Greece, Kosovo, Montenegro, North Macedonia, Romania, Serbia, Slovenia, and the European part of Turkey. In total, 553 records of traditional use were documented. The results reveal that Thymus taxa are most frequently employed for the treatment of respiratory and gastrointestinal disorders, followed by applications related to the nervous, urinary, cardiovascular, and integumentary systems. Culinary uses such as spices, herbal teas, beverages, and food preservatives are widely reported, highlighting the close connection between medicinal and dietary traditions. The analysis also identifies significant taxonomic inconsistencies in the ethnobotanical literature, particularly the frequent misapplication of names such as T. serpyllum and T. vulgaris, which complicates the interpretation of traditional knowledge. Overall, the strong cross-cultural consistency of uses across Balkan countries supports the pharmacological relevance of Thymus species and aligns well with modern phytotherapeutic evaluations. This review underscores the importance of critically integrating ethnobotanical data, taxonomic accuracy, and contemporary phytotherapy to better understand and utilize the therapeutic potential of Thymus species. Full article

Ashwagandha (Withania somnifera L.) root powder and extracts have long been used in Ayurvedic medicine to improve sleep and anxiety. Recent scientific investigations into its efficacy have shown promise for relief from anxiety, insomnia and stress and for improving the immune system. It has also been suggested that oxygen uptake in the cardiovascular system, muscle strength, cognitive function, the reproductive system and the aging process significantly benefit from ashwagandha treatment. Since the herbal remedy is taken daily by millions of people in India, China and parts of the West, it is interesting that there are very few case reports of side effects directly attributed to the treatment, suggesting that the administration of ashwagandha preparations may be safe. Currently, neither the European Medicines Agency nor the FDA considers ashwagandha as a drug or general health supplement. Therefore, ashwagandha products are marketed in the West as dietary supplements so that users may be exposed to unscrupulous vendors. In this narrative/literature review, scientific findings from basic research and human clinical trials on herbal remedies, spanning the period from 1994 to date, were critically evaluated for the purpose of highlighting knowledge gaps to provide context for new research. Such investigations will provide evidence for the efficacy and safety of ashwagandha treatment, thus making the herbal preparations more accessible to a wider audience. Full article

Polyphenols are key dietary bioactive compounds, reducing oxidative stress and neurodegeneration. This study investigated the in vitro antioxidant and neuroprotective potential of some edible fruits (apricots, plums, figs) and vegetable (parsley) extracts related to their phytochemical profile. Plum extract exhibited the strongest antioxidant capacity (ABTS IC₅₀ 1.733 ± 0.079 mg/g; DPPH IC₅₀ 1.593 ± 0.069 mg/g; FRAP 23.161 ± 1.094 mM Fe²⁺), linked to its high chlorogenic and caffeic acids content. Parsley displayed the most potent AChE inhibition (IC₅₀ 0.825 ± 0.026 mg/g), associated with an elevated flavonoids level (TFC 12.874 ± 0.534 mg QE/g) and the presence of ferulic and vanillic acids. Apricot was characterized by notable gallic, syringic, and chlorogenic acids, supporting moderate neuroprotective potential. Figs showed weaker radical scavenging ability but provided a balanced profile of protocatechuic, caffeic, and syringic acids. Correlation analysis revealed specific compound–activity associations, including syringic and vanillic acids with DPPH scavenging capacity, p-coumaric acid with TPC, and gallic/ferulic acids with AChE inhibition. Effect-directed HPTLC confirmed chlorogenic acid as a major contributor to the antioxidant capacity. To our knowledge, this is the first study to comparatively integrate spectrophotometric antioxidant assays, UHPLC-based quantitative phenolic profiling, effect-directed HPTLC bioautography, and AChE inhibition analysis across three edible fruits and one vegetable frequently co-consumed in Mediterranean-type diets, enabling a cross-species compound–activity correlation framework. These species exhibit distinct but complementary phytochemical and biofunctional profiles. Their combined use may support the formulation of functional foods with synergistic antioxidant and neuroprotective benefits. Full article