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19 pages, 599 KiB  
Article
Effective Seed Scheduling for Directed Fuzzing with Function Call Sequence Complexity Estimation
by Xi Peng, Peng Jia, Ximing Fan, Cheng Huang and Jiayong Liu
Appl. Sci. 2025, 15(15), 8345; https://doi.org/10.3390/app15158345 - 26 Jul 2025
Viewed by 257
Abstract
Directed grey-box fuzzers focus on testing specific target code. They have been utilized in various security applications, such as reproducing known crashes and identifying vulnerabilities resulting from incomplete patches. Distance-guided directed fuzzers calculate the distance to the target node for each node in [...] Read more.
Directed grey-box fuzzers focus on testing specific target code. They have been utilized in various security applications, such as reproducing known crashes and identifying vulnerabilities resulting from incomplete patches. Distance-guided directed fuzzers calculate the distance to the target node for each node in a CFG or CG, which has always been the mainstream in this field. However, the distance can only reflect the relationship between the current node and the target node, and it does not consider the impact of the reaching sequence before the target node. To mitigate this problem, we analyzed the properties of the instrumented function’s call graph after selective instrumentation, and the complexity of reaching the target function sequence was estimated. Assisted by the sequence complexity, we proposed a two-stage function call sequence-based seed-scheduling strategy. The first stage is to select seeds with a higher probability of generating test cases that reach the target function. The second stage is to select seeds that can generate test cases that meet the conditions for triggering the vulnerability as much as possible. We implemented our approach in SEZZ based on SelectFuzz and compare it with related works. We found that SEZZ outperformed AFLGo, Beacon, WindRanger, and SelectFuzz by achieving an average improvement of 13.7×, 1.50×, 9.78×, and 2.04× faster on vulnerability exposure, respectively. Moreover, SEZZ triggered three more vulnerabilities than the other compared tools. Full article
(This article belongs to the Special Issue Cyberspace Security Technology in Computer Science)
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30 pages, 5051 KiB  
Article
Design and Validation of an Active Headrest System with Integrated Sensing in Rear-End Crash Scenarios
by Alexandru Ionut Radu, Bogdan Adrian Tolea, Horia Beles, Florin Bogdan Scurt and Adrian Nicolaie Tusinean
Sensors 2025, 25(14), 4291; https://doi.org/10.3390/s25144291 - 9 Jul 2025
Viewed by 321
Abstract
Rear-end collisions represent a major concern in automotive safety, particularly due to the risk of whiplash injuries among vehicle occupants. The accurate simulation of occupant kinematics during such impacts is critical for the development of advanced safety systems. This paper presents an enhanced [...] Read more.
Rear-end collisions represent a major concern in automotive safety, particularly due to the risk of whiplash injuries among vehicle occupants. The accurate simulation of occupant kinematics during such impacts is critical for the development of advanced safety systems. This paper presents an enhanced multibody simulation model specifically designed for rear-end crash scenarios, incorporating integrated active headrest mechanisms and sensor-based activation logic. The model combines detailed representations of vehicle structures, suspension systems, restraint systems, and occupant biomechanics, allowing for the precise prediction of crash dynamics and occupant responses. The system was developed using Simscape Multibody, with CAD-derived components interconnected through physical joints and validated using controlled experimental crash tests. Special attention was given to modelling contact forces, suspension behaviour, and actuator response times for the active headrest system. The model achieved a root mean square error (RMSE) of 4.19 m/s2 and a mean absolute percentage error (MAPE) of 0.71% when comparing head acceleration in frontal collision tests, confirming its high accuracy. Validation results demonstrate that the model accurately reproduces occupant kinematics and head acceleration profiles, confirming its reliability and effectiveness as a predictive tool. This research highlights the critical role of integrated sensor-actuator systems in improving occupant safety and provides a flexible platform for future studies on intelligent vehicle safety technologies. Full article
(This article belongs to the Special Issue Intelligent Sensors for Smart and Autonomous Vehicles)
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32 pages, 11290 KiB  
Article
Material Characterization and Stress-State-Dependent Failure Criteria of AASHTO M180 Guardrail Steel: Experimental and Numerical Investigation
by Qusai A. Alomari, Tewodros Y. Yosef, Robert W. Bielenberg, Ronald K. Faller, Mehrdad Negahban, Zesheng Zhang, Wenlong Li and Brandt M. Humphrey
Materials 2025, 18(11), 2523; https://doi.org/10.3390/ma18112523 - 27 May 2025
Viewed by 544
Abstract
As a key roadside safety feature, longitudinal guardrail steel barriers are purposefully designed to contain and redirect errant vehicles to prevent roadway departure, dissipate impact energy through plastic deformation, and reduce the severity of vehicle crashes. Nevertheless, these systems should be carefully designed [...] Read more.
As a key roadside safety feature, longitudinal guardrail steel barriers are purposefully designed to contain and redirect errant vehicles to prevent roadway departure, dissipate impact energy through plastic deformation, and reduce the severity of vehicle crashes. Nevertheless, these systems should be carefully designed and assessed, as localized rupturing, especially near splice or impact locations, can lead to catastrophic failures, compromising vehicle containment, violating crash safety standards, and ultimately jeopardizing the safety of occupants and other road users. Before conducting full-scale crash testing, finite element analysis (FEA) tools are widely employed to evaluate the design efficiency, optimize system configurations, and preemptively identify potential failure modes prior to expensive physical crash testing. To accurately assess system behavior, calibrated material models and precise failure criteria must be utilized in these simulations. Despite the existence of numerous failure criteria and material models, the material characteristics of AASHTO M-180 guardrail steel have not been fully investigated. This paper significantly advances the FE modeling of ductile fracture in guardrail steel, addressing a critical need within the roadside safety community. This study formulates stress-state-dependent failure criteria and proposes advanced material modeling techniques. Extensive experimental testing was conducted on steel specimens having various triaxiality and Lode parameter values to reproduce a wide spectrum of complex, three-dimensional stress-state loading conditions. The test results were then used to identify material properties and construct a failure surface. Subsequent FEA, which incorporated the Generalized Incremental Stress-State-Dependent Damage Model (GISSMO) in conjunction with two LS-DYNA material models, illustrates the capability of the developed surface and material input parameters to predict material behavior under various stress states accurately. A parametric study was completed to further validate the proposed models, highlighting their robustness and reliability. Full article
(This article belongs to the Special Issue From Materials to Applications: High-Performance Steel Structures)
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12 pages, 20754 KiB  
Article
Development of a New Electric Vehicle Post-Crash Fire Safety Test in Korea (Proposed for the Korean New Car Assessment Program)
by Jeongmin In, Jaehong Ma and Hongik Kim
World Electr. Veh. J. 2025, 16(2), 103; https://doi.org/10.3390/wevj16020103 - 13 Feb 2025
Viewed by 1825
Abstract
Recent fire incidents following electric vehicle (EV) collisions have been increasing rapidly in Korea, corresponding to the growing distribution of EVs. While the overall number of EV fires is lower compared to those involving internal combustion engine (ICE) vehicles, EV fires can lead [...] Read more.
Recent fire incidents following electric vehicle (EV) collisions have been increasing rapidly in Korea, corresponding to the growing distribution of EVs. While the overall number of EV fires is lower compared to those involving internal combustion engine (ICE) vehicles, EV fires can lead to more severe outcomes. Current regulations for post-crash fuel system integrity evaluation do not differentiate between EVs and ICE vehicles. However, the causes of fires in these vehicles differ due to variations in the design and construction of their fuel systems. This study analyzed seventeen cases of EV post-crash fires in Korea to derive two representative risk scenarios for EV post-crash fires. The first scenario involves significant intrusion into the EV front-end structure resulting from high-speed frontal collisions, while the second scenario involves direct impacts to the battery pack mounted under the vehicle from road curbs at low speeds (30–40 km/h). Based on these scenarios, we conducted tests to assess battery damage severity under two crash test modes, simulating both high-speed frontal collisions and low-speed curb impacts. The test results led to the development of a draft crash test concept to evaluate EV post-crash fire risks. Furthermore, we assessed the reproducibility of these test modes in relation to actual EV post-crash fires. Our findings indicate that square-shaped impactors provide higher reproducibility in simulating real EV post-crash fire incidents compared to hemisphere-shaped impactors. Additionally, a fire occurred 31 days after the storage of a crash-evaluated battery test specimen, which was determined to be caused by moisture invasion during post-crash storage, accelerating a micro-short circuit. This study aims to contribute to the development of new evaluation methods for the Korean New Car Assessment Program (KNCAP) to enhance EV post-crash fire safety by utilizing these test results to refine collision severity evaluation methods. Full article
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17 pages, 10845 KiB  
Article
Simulation of Automobile Structural Member Deformation and Crash via Isogeometric Analysis
by Yuta Yokoyama, Kei Nagasaka, Idemitsu Masuda, Hirofumi Sugiyama and Shigenobu Okazawa
Vehicles 2024, 6(2), 967-983; https://doi.org/10.3390/vehicles6020046 - 15 Jun 2024
Cited by 1 | Viewed by 1715
Abstract
We conducted an isogeometric analysis (IGA) to evaluate the performance of automobile structural member deformation and crash. In automobile crash analysis, ensuring the accuracy of the acceleration, velocity, and load in time series, as well as the structural deformation behavior, is important. To [...] Read more.
We conducted an isogeometric analysis (IGA) to evaluate the performance of automobile structural member deformation and crash. In automobile crash analysis, ensuring the accuracy of the acceleration, velocity, and load in time series, as well as the structural deformation behavior, is important. To maintain the aforementioned consistency, accurately reproducing the bending and buckling of structural members is indispensable. In this study, we firstly computed the bending and buckling of structural members using IGA and validated its performance by comparing the results with those of a conventional finite element analysis and experiments. In addition, we utilized IGA for the crash analysis of an automobile body. Full article
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15 pages, 21525 KiB  
Article
Vibration-Based Smart Sensor for High-Flow Dust Measurement
by Anibal Reñones, Cristina Vega and Mario de la Rosa
Sensors 2023, 23(11), 5019; https://doi.org/10.3390/s23115019 - 24 May 2023
Viewed by 1941
Abstract
Asphalt mixes comprise aggregates, additives and bitumen. The aggregates are of varying sizes, and the finest category, referred to as sands, encompasses the so-called filler particles present in the mixture, which are smaller than 0.063 mm. As part of the H2020 CAPRI project, [...] Read more.
Asphalt mixes comprise aggregates, additives and bitumen. The aggregates are of varying sizes, and the finest category, referred to as sands, encompasses the so-called filler particles present in the mixture, which are smaller than 0.063 mm. As part of the H2020 CAPRI project, the authors present a prototype for measuring filler flow, through vibration analysis. The vibrations are generated by the filler particles crashing to a slim steel bar capable of withstanding the challenging conditions of temperature and pressure within the aspiration pipe of an industrial baghouse. This paper presents a prototype developed to address the need for quantifying the amount of filler in cold aggregates, considering the unavailability of commercially viable sensors suitable for the conditions encountered during asphalt mix production. In laboratory settings, the prototype simulates the aspiration process of a baghouse in an asphalt plant, accurately reproducing particle concentration and mass flow conditions. The experiments performed demonstrate that an accelerometer positioned outside the pipe can replicate the filler flow within the pipe, even when the filler aspiration conditions differ. The obtained results enable extrapolation from the laboratory model to a real-world baghouse model, making it applicable to various aspiration processes, particularly those involving baghouses. Moreover, this paper provides open access to all the data and results used, as part of our commitment to the CAPRI project, with the principles of open science. Full article
(This article belongs to the Section Industrial Sensors)
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21 pages, 12714 KiB  
Article
Wear Mechanisms in Press Hardening: An Analysis through Comparison of Tribological Tests and Industrial Tools
by Jaume Pujante, Eduard Garcia-Llamas, Giselle Ramírez, Nuria Cuadrado, Agim Ademaj, Montserrat Vilaseca and Daniel Casellas
Lubricants 2023, 11(5), 222; https://doi.org/10.3390/lubricants11050222 - 16 May 2023
Cited by 2 | Viewed by 1959
Abstract
Press hardened components have become widespread in the automotive industry in structural and crash-resistant applications, thanks to the combination of the complex shapes and high mechanical properties obtained. However, the press hardening of coated boron steel results in severe adhesive-based wear, with tool [...] Read more.
Press hardened components have become widespread in the automotive industry in structural and crash-resistant applications, thanks to the combination of the complex shapes and high mechanical properties obtained. However, the press hardening of coated boron steel results in severe adhesive-based wear, with tool maintenance being required in as few as 3000 cycles. The current industrial implementation of press hardening is defined to work around this phenomenon. While this aspect has been studied by different authors, most of the literature deals with laboratory-scale tribosimulators, leaving an open question into how this knowledge transfers to macroscopic effects on the industrial process. In this work, wear in press hardening is studied by comparing the results obtained in laboratory conditions with a pilot-scale line, and finally, with wear mechanisms observed on industrial tools. The aim of this study is to consolidate the current knowledge about the micro-mechanisms involved, and to understand to what extent the existing tests reproduce the actual mechanisms observed in the press floor. The results show how material transfer mainly happens as an accumulation of dust compacted into initial defects on the tool surface. Moreover, this mechanism is effectively reproduced in laboratory tribosimulators and pilot environments, showing a similar morphology to wear on industrial tools. The work sheds light on the underlying causes of wear, and its potential mitigation strategies. Full article
(This article belongs to the Special Issue Tribology for Lightweighting)
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17 pages, 5009 KiB  
Article
Comparison of Volunteers’ Head Displacement with Computer Simulation—Crash Test with Low Speed of 20 km/h
by Damian Frej and Marek Jaśkiewicz
Sensors 2022, 22(24), 9720; https://doi.org/10.3390/s22249720 - 12 Dec 2022
Cited by 7 | Viewed by 3815
Abstract
Recently, the automotive industry has used simulation programs much more often than experimental research. Computer simulations are more and more often used due to the repeatability of simulation conditions and the possibility of making modifications in simulation objects. Experimental and simulation studies carried [...] Read more.
Recently, the automotive industry has used simulation programs much more often than experimental research. Computer simulations are more and more often used due to the repeatability of simulation conditions and the possibility of making modifications in simulation objects. Experimental and simulation studies carried out are aimed at developing a model of a simulation dummy adapted to both frontal and rear crash tests, taking into account changes in the moment of resistance in individual joints. The main purpose of the article is to reproduce a real crash test at a low speed of 20 km/h in a simulation program. For this purpose, a series of experimental crash tests with the participation of volunteers was carried out, and then a crash test with a dummy was simulated in the MSC ADAMS program. The experimental studies involved 100 volunteers who were divided into three percentile groups (C5, C50, C95). With the help of force sensors and a high-speed camera, crash tests of volunteers were recorded. The collected data from the force sensors made it possible to map the force in the seat belts. For low-speed crash tests, the displacement and acceleration of individual body parts of the dummy and volunteers can be measured using vision systems. The article identified head displacements of volunteers in the TEMA program based on a video analysis of a crash test film with a frequency of up to 2500 frames per second. The displacement of the simulation dummy’s head in the MSC ADAMS program in the considered crash time interval from 0.0 to 0.4 s for all three percentile groups coincided with the head displacement of the volunteers during the experimental crash test. Full article
(This article belongs to the Special Issue Sensors and Systems for Automotive and Road Safety)
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22 pages, 652 KiB  
Article
SwitchFuzz: Switch Short-Term Goals in Directed Grey-Box Fuzzing
by Ziheng He, Peng Jia, Yong Fang, Yuying Liu and Hairu Luo
Appl. Sci. 2022, 12(21), 11097; https://doi.org/10.3390/app122111097 - 2 Nov 2022
Cited by 1 | Viewed by 2024
Abstract
In recent years, fuzzing has become a powerful tool for security researchers to uncover security vulnerabilities. It is used to discover software vulnerabilities by continuously generating malformed inputs to trigger bugs. Directed grey-box fuzzing has also been widely used in the verification of [...] Read more.
In recent years, fuzzing has become a powerful tool for security researchers to uncover security vulnerabilities. It is used to discover software vulnerabilities by continuously generating malformed inputs to trigger bugs. Directed grey-box fuzzing has also been widely used in the verification of patch testing and in vulnerability reproduction. For directed grey-box fuzzing, the core problem is to make test cases reach the target and trigger vulnerabilities faster. Selecting seeds that are closer to the target site to be mutated first is an effective method. For this purpose, the DGF calculates the distance between the execution path and the target site by a specific algorithm. However, as time elapses in the execution process, the seeds covering a larger amount of basic blocks may be overlooked due to their long distances. At the same time, directed fuzzing often ignores the impact of coverage on test efficiency, resulting in a local optimum problem without accumulating enough valuable test cases. In this paper, we analyze and discuss these problems and propose SwitchFuzz, a fuzzer that can switch short-term goals during execution. SwitchFuzz keeps shortening the distance of test cases to reach the target point when it performs well and prioritizes reaching the target point. When positive feedback is not achieved over a period of time, SwitchFuzz tries to explore more possibilities. We compared the efficiency of SwitchFuzz with that of AFLGO in setting single target and multiple targets for crash recurrence in our experiments, respectively. The results show that SwitchFuzz produces a significant improvement over AFLGO in both the speed and the probability of triggering a specified crash. SwitchFuzz can discover more edges than AFLGO in the same amount of time and can generate seeds with smaller distances. Full article
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20 pages, 5176 KiB  
Article
Driver Behavioral Classification on Curves Based on the Relationship between Speed, Trajectories, and Eye Movements: A Driving Simulator Study
by Maria Emilia Schio Rondora, Ali Pirdavani and Ana Paula C. Larocca
Sustainability 2022, 14(10), 6241; https://doi.org/10.3390/su14106241 - 20 May 2022
Cited by 13 | Viewed by 3209
Abstract
Horizontal curves of rural highways are prone to a considerably high number of fatalities because an erroneous perception can lead to unsafe driving. This generally occurs when a driver fails to notice the highway geometry or changes in the driving environment, particularly curved [...] Read more.
Horizontal curves of rural highways are prone to a considerably high number of fatalities because an erroneous perception can lead to unsafe driving. This generally occurs when a driver fails to notice the highway geometry or changes in the driving environment, particularly curved segments. This study aimed to understand the geometric characteristics of curved segments, such as radius and approach tangents, on the driving performance towards minimizing vehicle crashes. Speed profiles and lateral position, the most common indicators of successful negotiation in curves, and eye movements were recorded during an experiment conducted in a fixed-base driving simulator equipped with an eye-tracking system with a road infrastructure (a three-lane highway) and its surroundings. A driving simulator can faithfully reproduce any situation and enable sustainable research because it is a high-tech and cost-effective tool allowing repeatability in a laboratory. The experiment was conducted with 28 drivers who covered approximately 500 test kilometers with 90 horizontal curves comprising nine different combinations of radii and approach tangent lengths. The drivers’ behavior on each curve was classified as ideal, normal, intermediate, cutting, or correcting according to their trajectories and speed changes for analyses of the performance parameters and their correlation conducted by factorial ANOVA and Pearson chi-square tests. The cross-tabulation results indicated that the safest behavior significantly increased when the curve radius increased, and the performance measures of curve radii were greatly affected. However, the driving behavior was not affected by the approach tangent length. The results revealed segments of the road that require a driver’s closer attention for essential vehicle control, critical information, and vehicle control in different parts of the task. Full article
(This article belongs to the Collection Emerging Technologies and Sustainable Road Safety)
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15 pages, 4245 KiB  
Article
NOG-Derived Peptides Can Restore Neuritogenesis on a CRASH Syndrome Cell Model
by Matteo Gasparotto, Yuriko Suemi Hernandez Gomez, Daniele Peterle, Alessandro Grinzato, Federica Zen, Giulia Pontarollo, Laura Acquasaliente, Giorgia Scapin, Elisabetta Bergantino, Vincenzo De Filippis and Francesco Filippini
Biomedicines 2022, 10(1), 102; https://doi.org/10.3390/biomedicines10010102 - 4 Jan 2022
Cited by 3 | Viewed by 2907
Abstract
Homo- and heterophilic binding mediated by the immunoglobulin (Ig)-like repeats of cell adhesion molecules play a pivotal role in cell-cell and cell-extracellular matrix interactions. L1CAM is crucial to neuronal differentiation, in both mature and developing nervous systems, and several studies suggest that its [...] Read more.
Homo- and heterophilic binding mediated by the immunoglobulin (Ig)-like repeats of cell adhesion molecules play a pivotal role in cell-cell and cell-extracellular matrix interactions. L1CAM is crucial to neuronal differentiation, in both mature and developing nervous systems, and several studies suggest that its functional interactions are mainly mediated by Ig2–Ig2 binding. X-linked mutations in the human L1CAM gene are summarized as L1 diseases, including the most diagnosed CRASH neurodevelopmental syndrome. In silico simulations provided a molecular rationale for CRASH phenotypes resulting from mutations I179S and R184Q in the homophilic binding region of Ig2. A synthetic peptide reproducing such region could both mimic the neuritogenic capacity of L1CAM and rescue neuritogenesis in a cellular model of the CRASH syndrome, where the full L1CAM ectodomain proved ineffective. Presented functional evidence opens the route to the use of L1CAM-derived peptides as biotechnological and therapeutic tools. Full article
(This article belongs to the Section Neurobiology and Clinical Neuroscience)
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20 pages, 11979 KiB  
Article
Oil Fumes, Flight Safety, and the NTSB
by Judith Anderson and Dieter Scholz
Aerospace 2021, 8(12), 389; https://doi.org/10.3390/aerospace8120389 - 10 Dec 2021
Cited by 3 | Viewed by 9237
Abstract
During its investigations into a series of ten aircraft crashes from 1979 to 1981, US National Transportation Safety Board (NTSB) officials were presented with a hypothesis that “several” of the crashes could have been caused by pilot impairment from breathing oil fumes inflight. [...] Read more.
During its investigations into a series of ten aircraft crashes from 1979 to 1981, US National Transportation Safety Board (NTSB) officials were presented with a hypothesis that “several” of the crashes could have been caused by pilot impairment from breathing oil fumes inflight. The NTSB and their industry partners ultimately dismissed the hypothesis. The authors reviewed the crash reports, the mechanics of the relevant engine oil seals, and some engine bleed air data to consider whether the dismissal was justified. Four of the nine aircraft crash reports include details which are consistent with pilot impairment caused by breathing oil fumes. None of the tests of ground-based bleed air measurements of a subset of oil-based contaminants generated in the engine type on the crashed aircraft reproduced the inflight conditions that the accident investigators had flagged as potentially unsafe. The NTSB’s conclusion that the hypothesis of pilot incapacitation was “completely without validity” was inconsistent with the evidence. Parties with a commercial conflict of interest should not have played a role in the investigation of their products. There is enough evidence that pilots can be impaired by inhaling oil fumes to motivate more stringent design, operation, and reporting regulations to protect safety of flight. Full article
(This article belongs to the Special Issue Aircraft Design (SI-3/2021))
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20 pages, 7660 KiB  
Article
Full-Scale Train-to-Train Impact Test and Multi-Body Dynamic Simulation Analysis
by Hui Zhao, Ping Xu, Benhuai Li, Shuguang Yao, Chengxing Yang, Wei Guo and Xianliang Xiao
Machines 2021, 9(11), 297; https://doi.org/10.3390/machines9110297 - 19 Nov 2021
Cited by 17 | Viewed by 4405
Abstract
When a train crashes with another train at a high speed, it will lead to significant financial losses and societal costs. Carrying out a train-to-train crash test is of great significance to reproducing the collision response and assessing the safety performance of trains. [...] Read more.
When a train crashes with another train at a high speed, it will lead to significant financial losses and societal costs. Carrying out a train-to-train crash test is of great significance to reproducing the collision response and assessing the safety performance of trains. To ensure the testability and safety of the train collision test, it is necessary to analyze and predict the dynamic behavior of the train in the whole test process before the test. This paper presents a study of the dynamic response of the train in each test stage during the train-to-train crash test under different conditions. In this study, a 1D/3D co-simulation dynamics model of the train under various load conditions of driving, collision and braking has been established based on the MotionView dynamic simulation software. The accuracy of the numerical model is verified by comparing with a five-vehicle formations train-to-train crash test data. Sensitivities of several key influencing parameters, such as the train formation, impact velocity and the vehicle mass, are reported in detail as well. The results show that the increase in the impact velocity has an increasing effect on the movement displacement of the vehicle in each process. However, increasing the vehicle mass and train formation has almost no effect on the running displacement of the braking process of the traction train. By sorting the variables in descending order of sensitivity, it can be obtained that impact speed > train formation > vehicle mass. The polynomial response surface method (PRSM) is used to construct the fitting relationship between the parameters and the responses. Full article
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29 pages, 2724 KiB  
Article
Dynamics of the Bacterial Community Associated with Phaeodactylum tricornutum Cultures
by Fiona Wanjiku Moejes, Antonella Succurro, Ovidiu Popa, Julie Maguire and Oliver Ebenhöh
Processes 2017, 5(4), 77; https://doi.org/10.3390/pr5040077 - 7 Dec 2017
Cited by 18 | Viewed by 10216
Abstract
The pennate diatom Phaeodactylum tricornutum is a model organism able to synthesize industrially-relevant molecules. Commercial-scale cultivation currently requires large monocultures, prone to bio-contamination. However, little is known about the identity of the invading organisms. To reduce the complexity of natural systems, we systematically [...] Read more.
The pennate diatom Phaeodactylum tricornutum is a model organism able to synthesize industrially-relevant molecules. Commercial-scale cultivation currently requires large monocultures, prone to bio-contamination. However, little is known about the identity of the invading organisms. To reduce the complexity of natural systems, we systematically investigated the microbiome of non-axenic P. tricornutum cultures from a culture collection in reproducible experiments. The results revealed a dynamic bacterial community that developed differently in “complete” and “minimal” media conditions. In complete media, we observed an accelerated “culture crash”, indicating a more stable culture in minimal media. The identification of only four bacterial families as major players within the microbiome suggests specific roles depending on environmental conditions. From our results we propose a network of putative interactions between P. tricornutum and these main bacterial factions. We demonstrate that, even with rather sparse data, a mathematical model can be reconstructed that qualitatively reproduces the observed population dynamics, thus indicating that our hypotheses regarding the molecular interactions are in agreement with experimental data. Whereas the model in its current state is only qualitative, we argue that it serves as a starting point to develop quantitative and predictive mathematical models, which may guide experimental efforts to synthetically construct and monitor stable communities required for robust upscaling strategies. Full article
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