Search Results (90)

Gastrointestinal (GI) parasites not only significantly impact goat health and productivity but can also affect human health due to the zoonotic potential of some species. This study investigates the prevalence of internal parasites within the tropical monsoon ecosystem of southern Thailand, focusing on both phenotypic and molecular characteristics of the parasites and identifying associated risk factors in caprine farming systems. A total of 276 meat goats from Nakhon Si Thammarat province were examined, indicating an overall GI parasite prevalence of 88.8% (245/276), with strongyles and Eimeria spp. identified as the dominant parasites. In addition, mixed parasitic infections were observed in 72.2% of cases, whereas single infections comprised 27.8%. Strongyle-positive fecal samples were cultured and genetically sequenced, revealing the presence of Haemonchus contortus, Trichostrongylus colubriformis, and Oesophagostomum asperum. For associated risk factors, gender and grazing with other herds significantly impacted overall GI parasitic infections, while the gender, breed, and packed cell volume (PCV) affected the strongyle infection. A correlation analysis revealed a substantial relationship between strongyle egg per gram (EPG) counts and clinical parameters, indicating that monitoring animals with low body condition scores (BCS) and high Faffa Malan Chart (FAMACHA) scores could be an effective strategy for controlling strongyle infections. These findings highlight the importance of continued research and effective farm management practices to address strongyle infections in meat goats, improving their health and agricultural productivity in tropical regions. Moreover, the detection of four zoonotic parasites (Giardia spp., H. contortus, T. colubriformis, and Fasciola spp.) indicates the necessity for the routine surveillance and monitoring of zoonotic parasites in goats to mitigate potential human health risks. Full article

Coccidian parasites possess complex life cycles involving asexual proliferation followed by sexual development, producing oocysts that are transmitted from host to host through feces, guaranteeing disease transmission. Eimeria necatrix is a highly pathogenic coccidian causing high mortality in birds. This study examined ultrastructural changes occurring during the third merogony, microgametogenesis, and macrogametogenesis of E. necatrix. The third-generation meront contained eight merozoites, each with coccidian-specific features like conoid, rhoptries, micronemes, and dense granules. Microgametes had a nucleus, mitochondrion, two flagella, and a basal apparatus. Macrogametes surrounded by two membranes (M1 and M2), contained organelles like WFB1, WFB2, endoplasmic reticulum, mitochondria, and tubular structures. Oocyst wall formation began with M2 separating from M1 and forming a loose veil around the organism. The WFB1 fused together to form the outer layer of the oocyst wall between M1 and M2, while M4 formed beneath M1. The WFB2 fused with the M4 to discharge its contents external to M4, which fused together to form the inner layer of the oocyst wall. Immunogold electron microscopy co-localization result showed that EnGAM22 localized to WFB1 and the outer wall, while EnGAM59 localized to WFB2 and the inner wall, suggesting they are key structural components of the oocyst wall. Full article

This retrospective study investigated the clinical presentation, diagnostic findings, and treatment outcomes in 419 backyard (pet) chickens admitted to the Service for Avian and Reptiles at the University of Veterinary Medicine Vienna from 1 May 2009 to 30 April 2019. The median age of the chickens was 1.5 years, with 83.8% being female. The majority (80.0%) presented with individual clinical issues, while 20.0% were diagnosed with flock diseases. The most common admission causes included unspecific clinical signs (57.3%), respiratory tract issues (18.4%), and locomotor problems (16.2%). Diagnostic imaging modalities such as ultrasonography, radiography, and CT scans revealed pathologies, including ascites, salpingitis, and skeletal system abnormalities. Parasitological examinations found a high rate of coccidia, Capillaria, and Ascaridia, while microbiological analysis identified Escherichia coli and Pasteurella multocida as common bacterial pathogens. The most prevalent diseases were upper respiratory tract infections (13.8%), egg peritonitis (9.9%), and soft tissue trauma (9.4%). Treatment outcomes showed that 67.5% survived to discharge, while 32.2% died or were euthanized. Surgical interventions were performed in 25.3% of cases, with the most common surgeries being salpingohysterectomy and wound debridement. This study highlights the wide range of health issues faced by pet chickens and emphasizes the importance of accurate diagnosis and targeted treatment in avian veterinary care. It also underscores the role of various diagnostic tools, such as imaging, pathogen detection, and histopathology, in addressing the health challenges of backyard chickens. Full article

Parasitic protozoa such as Lankesterella and Isospora are common in avian hosts, particularly in passerines. Despite their high prevalence, the diversity of these parasites within avian populations remains poorly understood. This study aimed to assess the diversity of Lankesterella and Isospora in passerine birds, using the SSU rRNA gene to characterize and compare the genetic variation in both parasites across multiple avian host species. For Isospora, the extraintestinal blood stages and oocysts from feces were compared. Minimum spanning networks were constructed to visualize haplogroups in relation to host specificity and to reveal the identity of various developmental stages. A total of 122 sequences from eight passerine species were used to generate a haplotype network for Lankesterella, and a total of 103 sequences (64 from blood and 39 from feces) was used for Isospora. We detected a total of 36 haplotypes for Lankesterella and 33 haplotypes for Isospora. In Lankesterella, we confirmed that the sedge warbler has its own specific lineages, whereas other warbler species share lineages belonging to three haplogroups; blue, great, marsh and willow tits have their own unique groups of lineages. Isospora is less host-specific than Lankesterella in avian hosts; nevertheless, Isospora sequences from blood and feces were identical in their respective hosts. Our findings provide insights into the diversity and host specificity of blood coccidians; moreover, we molecularly characterized the developmental stages of Isospora. Full article

The aim of the study was to determine the extent and intensity of gastrointestinal parasite infections in alpacas (Vicugna pacos) in Poland, considering factors such as region, gender, age, and season. The research was conducted on 29 herds located throughout Poland. Faecal samples from 512 alpacas were collected in autumn 2023 and spring 2024. Examination was performed using the direct flotation and McMaster methods. The average prevalence of gastrointestinal parasitic infections was 74.4%; 68.8% of alpacas were infected with nematodes and 34.8% with protists of the genus Eimeria. The most commonly detected parasites were Nematodirus spp. (38.5%) and Eimeria punoensis (18.2%). The analysis revealed significant differences in the intensity of gastrointestinal parasitic infections depending on region, age, gender, and season, suggesting the need to tailor deworming programs to the specific requirements of different groups of alpacas. Furthermore, regional differences in the prevalence of Strongyloides sp. and Strongylida infections were observed, indicating a significant impact of local environmental conditions and husbandry practices on the risk of infection. Seasonal variations in the intensity of Eimeria infections, particularly in autumn, were also noted. Parasitic infections in alpacas in Poland are common and generally asymptomatic. These results highlight the need for the implementation of a comprehensive preventive program for controlling parasitic infection, including regular diagnostics, pasture management optimization, maintenance of hygiene, and selective use of antiparasitic drugs in alpacas. Full article

The phylum Apicomplexa includes endoparasites of fish worldwide, which cause parasitic infections that can adversely affect productivity in aquaculture. They are considered bioindicators of water pollution. Piscine apicomplexan parasites can be divided into two major groups: the intracellular blood parasites (Adeleorina) and the coccidians (Eimeriorina), which can infect the gastrointestinal tract and several organs. This work aims to compile, as completely as possible and for the first time, the available information concerning the species of coccidia (Apicomplexa: Conoidasida), which has been reported from freshwater fish. A comprehensive bibliographic search was performed using all available databases and fields, including Scopus, PubMed, and Google Scholar. In the freshwater fish found, there were 173 described species. This review demonstrates that freshwater fish’s eimeriid coccidia are better studied than adeleid coccidia. Studies of coccidian freshwater fish fauna indicate a high infection with Eimeria and Goussia species. The wealthiest coccidia fauna were found in the Cypriniformes, Perciformes, Siluriformes and Cichliformes fishes. Full article

The effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) and coccidia vaccine challenge (CVC, Coccivac B-52™) on broilers fed a corn–SBM (CS) and a wheat–CS (WCS)-based diet was examined in this study. On day 14, 448 Cobb by-product breeder male broiler chickens were assigned to treatments (factorial arrangement) in a completely randomized design, with each treatment replicated seven times. Treatment effect was evaluated within each diet type as a 2 (enzyme levels) x 2 (CVC, 0 or 20X) factorial arrangement of treatments 7 and 14 days post-CVC. The 7-day (days 14–21) post-CVC, BWG, and feed efficiency (birds on the CS-based diet) were lower (p < 0.05), while birds on enzyme-supplemented diets had higher (p < 0.05) BWG compared to birds on diets without enzyme supplementation. Between days 21 and 28, an interaction between CVC and exogenous enzyme resulted in higher (p < 0.05) BWG compared with the challenged birds fed diets without enzyme supplementation. For birds fed WCS-based diets, CVC influenced (p < 0.05) BWG and feed efficiency (decreased days 14–21 and increased days 21–28), while CVC birds had higher BWG and feed efficiency 14 days post-CVC. Apparent ileal digestibility of dry matter, energy, and DE were lower (p < 0.05) in CVC broilers fed either the CS- or WCS-based diets (7 and 14 days post-CVC). Interaction between CVC and exogenous enzyme supplementation indicated that CVC, irrespective of exogenous enzyme supplementation with the WCS-based diet, decreased (p < 0.05) Ca utilization (7 days post-CVC) but increased (p < 0.05) Ca utilization compared to CVC birds without enzyme supplementation 14 days post-challenge. Seven days post-CVC, irrespective of the diet type, CVC resulted in lower (p < 0.05) duodenal VH and VH:CD and higher (p < 0.05) CD. Enzyme supplementation influenced (p < 0.05) duodenal CD (increased) and VH:CD (decreased) in birds fed the WCS-based diet. Results from this study showed that complete recovery from CVC was influenced by diet type, with CVC birds fed WCS-based diet having higher BWG and feed efficiency compared to the unchallenged birds. Full article

Background: Two high primary-immunization doses of a wild-type E. tenella strain were assessed in healthy pullets (5K versus 10K sporulated oocysts/bird) to understand the effects of coccidia infection. Methods: Acquired immunity was evaluated following primary immunization and two booster doses with the homologous strain. Total oocyst shedding, clinical signs, and viability of every bird/group after each immunization/booster were recorded. Indirect ELISA measured the time course of humoral responses from each immunization group against sporozoite and second-generation merozoite of E. tenella. Antigen pattern recognition on these two asexual zoite stages of E. tenella was analyzed using Western blotting with antibodies from each immunization program. Afterwards, antigen recognition of specific life-cycle stages was performed using individual pullet serums from the best immunization program. Results: A primary-immunization dose of 1 × 10⁴ oocysts/bird reduced the oocyst output; however, all pullets exhibited severe clinical signs and low specific antibodies titers, with decreased polypeptide recognition on both E. tenella asexual zoite stages. In contrast, immunization with 5 × 10³ oocysts/bird yielded the best outcomes regarding increased oocyst collection and early development of sterilizing immunity. After the first booster dosage, this group’s antisera revealed a strong pattern of specific antigen recognition on the two assayed E. tenella life-cycle stages. Conclusions: The E. tenella-specific antibodies from the 5 × 10³ oocysts/bird immunization program can aid in passive immunization trials and further research to identify B-cell immunoprotective antigens, which could help in the development of a genetically modified anticoccidial vaccine. Full article

Intestinal disease is becoming increasingly prevalent in poultry production; however, the effect of BEC in laying hens challenged with C. perfringens and coccidia is limited. This study aimed to investigate the effects of dietary supplementation with BEC on intestinal and ovarian health in laying hens challenged with C. perfringens and coccidia. A total of 80 Lohmann gray hens (35 weeks) were randomly assigned to two dietary groups supplemented with BEC (0 or 1000 mg/kg). Each group contained 40 replicates, with one bird each (one hen per cage). During the sixth week of the trial, half of the laying hens in each group (n = 20) were administered 40 mL C. perfringens (2.5 × 10¹⁰ CFU/mL) and 0.15 mL coccidia (55,000 sporangia/mL), while the other half (n = 20) were administered 40 mL phosphate-buffered saline (PBS). The results indicated that those challenged with C. perfringens and coccidia had severely damaged jejunal and ovarian histopathological morphology, increased oxidative damage, decreased cecal acetic acid and butyric acid content (p < 0.05), and resulted in lower gut microbial richness and diversity. The diet of 1000 mg/kg BEC reduced the jejunal and ovarian pathological damage and oxidative damage, increased short-chain fatty acids (SCFAs) content, and enhanced gut microbial richness and diversity (p < 0.05) in laying hens challenged with C. perfringens and coccidia. Furthermore, the positive effects of BEC on intestinal health were associated with changes in gut microbial composition and structure. In summary, dietary supplementation with BEC has the potential to reduce the severity of intestinal and ovarian damage caused by challenges posed by C. perfringens and coccidia through the modulation of gut microbiota. Full article

Eimeria christenseni is considered among the most pathogenic Eimeria species in goats. The aim of this study was to isolate an E. christenseni strain and to assess its infectivity, pathogenicity, and ability to develop a protective immune response. After previous collection of E. christenseni-positive faeces, purification of oocysts, and amplification in donor animals, an experimental infection was carried out. A total of 19 kids were divided into three groups: primary-infected and challenged, challenge control, and uninfected control. Infections were performed orally with 2 × 10⁵ sporulated oocysts per animal. Oocyst shedding, clinical signs, and production parameters, in addition to haematological and histopathological features, were monitored. The results showed that the Gran Canaria (GC) E. christenseni strain had similar morphological and biological characteristics to those previously described, but no significant clinical signs were observed despite the high oocyst counts here recorded. The novel strain isolated would therefore be of low pathogenicity but still able to develop significant immunoprotective responses upon challenge infections. Its biological similarities to highly pathogenic species such as Eimeria ninakohlyakimovae and Eimeria arloingi might enable comparative studies aimed at developing alternative strategies for drug treatments, including Eimeria species (strain)-specific vaccination strategies for the efficient control of goat coccidiosis. Full article

Swine coccidiosis is a widespread disease caused by species of the apicomplexan parasites Eimeria and Cystoisospora. Coccidiosis is a common cause of diarrhea in suckling piglets worldwide that directly reduces piglets’ immunity and increases the risk of infection with other enteropathogens, leading to increased clinical infection and mortality and consequent economic losses in the global pig industry. We searched the available literature to date, including English and Chinese articles, using six electronic bibliographic databases, including PubMed, ScienceDirect, Web of Science, CNKI, VIP Chinese Journal Database, and Wanfang Data. A standard approach for conducting scoping reviews was used to identify studies on the species and distribution of genus Sus coccidia worldwide. A quality assessment was done for each study reviewed and relevant information reported in the identified studies was collated, categorized, and summarized. A total of 149 publications and references were eligible for the final review. The distribution of 18 species of genus Sus coccidia recorded in 63 countries was collated. These included 15 Eimeria and 3 Cystoisospora species. C. suis was found in 48 countries, E. debliecki in 45 countries, E. scabra in 33 countries, E. polita in 31 countries, E. suis in 28 countries, E. perminuta in 26 countries, E. porci in 24 countries, E. neodebliecki and E.spinosa in 21 countries each, E.guevarai in 5 countries, C. almataensis in 4 countries, E. betica in 2 countries, and E. almataensis, E. ibrahimovae, E. residualis, E. szechuanensis, E. yanglingensis, and C. sundarbanensis were each found in only 1 country. Each species was listed according to its scientific name, host name, finding location, and geographical distribution. This review reflects the distribution and infection of genus Sus coccidia worldwide and provides more complete basic information to aid our understanding of the species and geographical distribution of coccidia in the genus Sus. Full article

The research aimed to investigate the perinatal pathology of Alpine ibex (Capra ibex) through the study of four young subjects (at the age of 3 to 4 months) found dead in Valle d’Aosta, a region of northwestern Italy. The carcasses were submitted to necropsy followed by an examination of ecto- and endoparasites (ECP and ENP); samples from the gross lesions (in summary, cutaneous papilloma and crusts, ocular discharge, lobular haemorrhagic areas in the lungs, catarrhal–haemorrhagic enterocolitis) were analysed by bacteriological, histopathological, and biomolecular methods to define the etiological agent. The subjects, with various co-infection patterns, were affected by contagious ecthyma virus (ORFV) (agent of a highly diffusive pustular dermatitis transmissible to small ruminants and humans), Enteropathogenic Escherichia coli (EPEC) (major etiological agent of infantile diarrhoea especially in developing countries), Mycoplasma conjunctivae (MC) (cause of an ocular infection common to goats and sheep), various ECP (ticks and keds) and ENP (lung and intestinal nematodes, and coccidia). This study emphasises the potential role of the Alpine ibex in the transmission of infectious diseases to other animals such as to humans and, secondly, the need to apply diversified analytical approaches, with the commitment of various specialistic skills, in order to define, in detail, the various and frequently overlapping causes that led a free-ranging animal to the death. Full article

The order Passeriformes is the richest and most abundant group of birds, but despite numerous parasites being identified in wild birds, this order has received limited focus. This study analyzed 17 passerines admitted to the Grupo de Rehabilitación de la Fauna Autóctona y su Hábitat (GREFA), a wildlife rehabilitation center in Spain, during October to December 2022. Necropsies were conducted to determine the presence of parasites, and intestinal contents were analyzed using fecal smear, flotation and sedimentation techniques and the McMaster method. Sixteen passerines (94.1%) were positive for parasites. Identified species included Monojoubertia microhylla and the genera Ornithonyssus sp., Diplotriaena spp., Serratospiculum sp., Porrocaecum sp., Capillaria spp., Syngamus sp., Strongyloides sp. and Brachylecithum sp., besides cestodes and coccidia. The comparative analysis of parasitological diagnostic techniques showed that the Willis flotation technique was effective for detecting coccidia. However, to obtain more accurate results for other parasites, it is important to complement this technique with direct examination or sedimentation techniques. Among the 12 passerines positive for coccidia, oocyst counts per gram of intestinal contents ranged from 100 to 30,450, with a median of 7350. This study provides valuable insights into the parasitic fauna of Passeriformes, serving as a cornerstone for future research and enhancing our understanding of these avian species. Full article

Coccidiosis is a protozoan disease that causes diarrhea in cattle. This study examines the impact of treating pregnant cows at dry-off with melatonin on postpartum coccidia excretion in dams and their calves. The study population comprised 106 primiparous lactating dairy cows: 53 controls and 53 receiving melatonin on days 220–226 of gestation, plus 99 calves: 52 born from control and 47 from treated dams. Feces samples were collected from each dam on gestation days 220–226 and on days 10–16 and 30–36 postpartum; and from each calf on days 10–16 and 30–36 of age. Postpartum rates of high excretion of oocysts per gram (OPG) (feces counts > 5000) were significantly lower (p < 0.01) in treated than control dams. Low excretion rates of OPG (<2000) were significantly higher (p < 0.01) in the melatonin treatment than control groups in dams on days 30–36 of lactation and in calves at 10–16 and 30–36 days of life. In conclusion, melatonin treatment in lactating cows at dry-off reduced coccidia shedding in dams and their calves during the early postpartum period. Full article

Coccidiosis, caused by different species of Eimeria parasites, is an economically important disease in poultry and livestock worldwide. This study aimed to investigate the changes in the ileal microbiota and fecal metabolites in chickens after repeated infections with low-dose E. mitis. The chickens developed solid immunity against a high dose of E. mitis infection after repeated infections with low-dose E. mitis. The composition of the ileal microbiota and the metabonomics of the Eimeria-immunized group and the control group were detected using 16S rRNA sequencing and liquid chromatography–mass spectrometry (LC-MS). The relative abundance of Neisseria, Erysipelotrichaceae, Incertae sedis, Coprobacter, Capnocytophaga, Bifidobacterium, and the Ruminococcus torques group declined in the Eimeria-immunized chickens, whereas Alloprevotella, Staphylococcus, Haemophilus, and Streptococcus increased. Furthermore, 286 differential metabolites (including N-undecylbenzenesulfonic acid, 1,25-dihydroxyvitamin D3, gluconic acid, isoleucylproline, proline, and 1-kestose) and 19 significantly altered metabolic pathways (including galactose metabolism, ABC transporters, starch and sucrose metabolism, the ErbB signaling pathway, and the MAPK signaling pathway) were identified between the Eimeria-immunized group and the control group. These discoveries will help us learn more about the composition and dynamics of the gut microbiota as well as the metabolic changes in chickens infected with Eimeria spp. Full article