Search Results (14)

Fiber-based sorbent materials are an essential part of containing oil spills, thus preventing ecological damage. Poly(styrene-b-isobutylene-b-styrene) thermoplastic elastomer fibers were successfully produced by centrifugal spinning. Scanning electron microscopy revealed that the fibers were bead free and smooth-surfaced, with an average fiber diameter of 5.9 ± 2.3 μm. Contact angle measurements proved the highly hydrophobic (water contact angle of 126.8 ± 6.4°) and highly oleophilic nature of the fiber mat. The sorption and retention capacities of the fiber mat were tested for various oils and benchmarked against polypropylene as the industry standard and polystyrene, which is widely used in the literature. The oil uptake of the fiber mat showed a strong correlation with the viscosity of the oil, resulting in sorption capacities of 10.1 ± 0.8 g/g for sunflower oil, 19.9 ± 2.1 g/g for motor oil, and 23.8 ± 1.8 g/g for gear oil. Oil–water separation tests were also conducted, resulting in ~100% oil removal. The thermoplastic elastomer fiber mat outperformed the industry standard; however, the polystyrene fiber mat demonstrated the best oil sorption performance. Full article

A low-cost, handheld centrifugal microfluidic system for multiplexed visual detection based on recombinase polymerase amplification (RPA) was developed. A concise centrifugal microfluidic chip featuring four reaction units was developed to run multiplexed RPA amplification in parallel. Additionally, a significantly shrunk-size and cost-effective handheld companion device was developed, incorporating heating, optical, rotation, and sensing modules, to perform multiplexed amplification and visual detection. After one-time sample loading, the metered sample was equally distributed into four separate reactors with high-speed centrifugation. Non-contact heating was adopted for isothermal amplification. A tiny DC motor on top of the chip was used to drive steel beads inside reactors for active mixing. Another small DC motor, which was controlled by an elaborate locking strategy based on magnetic sensing, was adopted for centrifugation and positioning. Visual fluorescence detection was optimized from different sides, including material, surface properties, excitation light, and optical filters. With fluorescence intensity-based visual detection, the detection results could be directly observed through the eyes or with a smartphone. As a proof of concept, the handheld device could detect multiple targets, e.g., different genes of African swine fever virus (ASFV) with the comparable LOD (limit of detection) of 75 copies/test compared to the tube-based RPA. Full article

Cassava starch nanoparticles (SNP) were produced using the nanoprecipitation method after modification of starch granules using ultrasound (US) or heat–moisture treatment (HMT). To produce SNP, cassava starches were gelatinized (95 °C/30 min) and precipitated after cooling, using absolute ethanol. SNPs were isolated using centrifugation and lyophilized. The nanoparticles produced from native starch and starches modified using US or HMT, named NSNP, USNP and HSNP, respectively, were characterized in terms of their main physical or functional properties. The SNP showed cluster plate formats, which were smooth for particles produced from native starch (NSNP) and rough for particles from starch modified with US (USNP) or HMT (HSNP), with smaller size ranges presented by HSNP (~63–674 nm) than by USNP (~123–1300 nm) or NSNP (~25–1450 nm). SNP had low surface charge values and a V-type crystalline structure. FTIR and thermal analyses confirmed the reduction of crystallinity. The SNP produced after physical pretreatments (US, HMT) showed an improvement in lipophilicity, with their oil absorption capacity in decreasing order being HSNP > USNP > NSNP, which was confirmed by the significant increase in contact angles from ~68.4° (NSNP) to ~76° (USNP; HSNP). A concentration of SNP higher than 4% may be required to produce stability with 20% oil content. The emulsions produced with HSNP showed stability during the storage (7 days at 20 °C), whereas the emulsions prepared with NSNP exhibited phase separation after preparation. The results suggested that dual physical modifications could be used for the production of starch nanoparticles as stabilizers for Pickering emulsions with stable characteristics. Full article

Fibrous structures are promising candidates for oil–water separation applications. In this study, we have produced poly(styrene-b-isobutylene-b-styrene) thermoplastic elastomeric fibers with the centrifugal spinning fiber production method. The optimal fiber production conditions were achieved when using a 25% w/w solution concentration in an 80/20 tetrahydrofuran/toluene (w/w) solvent system at 8000 rpm rotational speed. The produced fibers were bead-free and smooth-surfaced with a diameter of 3.68 µm. The produced fibers were highly hydrophobic and oleophilic, suggested by a water contact angle of 129° and the instantaneous absorption of the oil droplet. The oil absorption study showed fast absorption kinetics with 94% relative oil uptake after 1 min and a maximum of 16.5 g sunflower oil/g fiber. The results suggest that polyisobutylene-based thermoplastic elastomers could be promising alternatives in oil absorption applications. Full article

Leukocyte–platelet-rich fibrin (L-PRF) contains growth factors that stimulate bone regeneration. This study evaluated the bone repair in a tibia rat model around two implant surfaces in combination or not with L-PRF by assessing microtomographic and histomorphometric parameters. A total of 48 female rats were used in the study, in which 24 received implants with two types of surface treatments (dual acid etched—DAE or nanohydroxyapatite—nanoHA), and the other 24 received the same mini implants with L-PRF, which was collected by cardiac puncture, centrifugated, and inserted in the bone bed. The animals were euthanized 7 and 30 days after implant placement, and the retrieved samples were prepared for microtomographic and histomorphometric (bone-to-implant contact—BIC; and Bone Area Fraction Occupancy—BAFO) analyses. The adhesion of the nanoHA surface onto the implant surface was investigated by insertion and removal in simulated bone medium (Sawbones). The adhesion evaluation revealed that the loss of nanoHA after this procedure (as measured with SEM) from the implant surface was less than 1%. Overall, the nanoHA surface presented more bone in contact and in proximity to the implant, a higher bone surface/tissue volume fraction, a higher number of bone trabeculae, as well as trabecular separation relative to the DAE surface. Such results were more evident when the nanoHA surface was combined with L-PRF and after 30 days in vivo. The nanoHA surface presented higher BAFO when compared to DAE, with or without association with L-PRF. Therefore, implants with a nanoHA surface potentially benefit from the association to L-PRF. Full article

Due to the importance of process intensification, modeling of Annular Centrifugal Contactors (ACCs) is becoming of increasing interest. By the current state of scientific knowledge, universal modeling without high computing power of these complex apparatuses is not possible to a satisfactory degree. In this article, a one-dimensional model to describe the mass transfer during a physical extraction process in an ACC is presented. The model is based on solely geometrical data and operating conditions of the ACC, as well as physical properties of the components. Regarding the selection of physical properties, only physical properties that are easily accessible were used. With this model, mass transfer calculations are possible and therefore, the output concentrations can be predicted. Simulations of an ACC based on the model were done by creating and running a python code. Validation of the model was conducted by varying and comparing operating conditions in both the simulation and the experiments. Validation was completed successfully for a representative system of components and showed good agreement over a range of rotational frequencies and temperatures. Full article

Due to the complexity of the working conditions and the diversity of application scenarios, the normal operation of a fan, whether volute tongue, volute shell surface, or blade, often encounters some unavoidable problems, such as flow separation, wear, vibration, etc.; the aerodynamic noise caused by these problems has a significant impact on the normal operation of the fan. However, despite the use of aerodynamic acoustics to design low-noise fans or the use of sound absorption, sound insulation, and sound dissipation as the main traditional noise control techniques, they are in a state of technical bottleneck. Thus, the search for more efficient methods of noise reduction is looking toward the field of bionics. For this purpose, this paper first analyzes the mechanism of fan noise in the volute tongue and blades, and then, this paper reviews the noise control mechanism and improvement research using the bionic structures in the volute tongue structure, the contact surface of the volute shell, and the leading and trailing edges of the blade in the centrifugal fan. Finally, the current challenges and prospects of bionic structures for aerodynamic noise control of centrifugal fans are discussed. Full article

This paper presents a detailed review of the streaming electrification phenomena of different insulating fluids for power transformers. The comparison of different techniques used to assess the charging tendency of fluids is discussed depending on the flow type (planar or centrifugal), volume of oil, and interface material. The charge separation between the insulating fluid and metallic/pressboard interfaces is explained in terms of the electrical double layer formation involving a fixed layer and diffuse layer. Based on the experimental results, the streaming electrification is observed to be a function of various factors such as speed, temperature, electric field, and surface roughness. Depending on the molecular structure of insulating liquids that come into contact with solid insulation at the interface, the streaming current can increase; hence, a suitable additive (benzotriazole, fullerene, Irgamet 39) is selected based on the type of fluid and charge polarity. The degradation of the insulating liquid upon ageing, which increases the streaming current and reclamation of such aged fluids using adsorbents (Fuller’s earth, activated carbon, bentonite, and alumina), is a possible method to suppress the static current through improving its dielectric properties. The nanofluids show a higher streaming current compared to base fluid with no change observed even after the reclamation process. The energization process using alternating current (AC) and direct current (DC) impacts the streaming phenomenon depending on its magnitude and polarity. The diffusion of sulfur compounds in the insulating liquid is another major hazard to transformers because the sulfide ions affect the physio-chemical reaction at the interface material, which is responsible for the formation of streaming current. Full article

The objective of this study is to develop data-driven predictive models for peak rotation and factor of safety for tipping-over failure of rocking shallow foundations during earthquake loading using multiple nonlinear machine learning (ML) algorithms and a supervised learning technique. Centrifuge and shaking table experimental results on rocking foundations have been used for the development of k-nearest neighbors regression (KNN), support vector regression (SVR), and random forest regression (RFR) models. The input features to ML models include critical contact area ratio of foundation; slenderness ratio and rocking coefficient of rocking system; peak ground acceleration and Arias intensity of earthquake motion; and a categorical binary feature that separates sandy soil foundations from clayey soil foundations. Based on repeated k-fold cross validation tests of models, we found that the overall average mean absolute percentage errors (MAPE) in predictions of all three nonlinear ML models varied between 0.46 and 0.60, outperforming a baseline multivariate linear regression ML model with corresponding MAPE of 0.68 to 0.75. The input feature importance analysis reveals that the peak rotation and tipping-over stability of rocking foundations are more sensitive to ground motion demand parameters than to rocking foundation capacity parameters or type of soil. Full article

The surface of a centrifugal microfluidic immunoassay system chip such as polymethyl methacrylate (PMMA) is often hydrophobic, which leads to problems such as poor liquid transfer efficiency and easy-to-block siphon channels, leading to bad fluid control. Therefore, surface hydrophilic modification for such chips is necessary to improve the rapidity and sensitivity of the system. Chemical modification is commonly used, but there is little research on the hydrophilic effect of different concentrations of hydrophilic reagents. According to function requirements for different microchannels of the chip (some only need to ensure the liquid can flow into the next chamber, and some also need to ensure the function of “closing the door” during immunoassay incubation), we explored the best combination of hydrophilic reagent and concentration through experiments. Firstly, three hydrophilic reagents were used for modification. Secondly, the hydrophilic effects of different reagents and concentrations were explored by contact angle test, the influence of different modification methods on liquid transfer efficiency was characterized by residual liquid calculation in the chamber. Finally, the effect of different hydrophilic reagents on absorbance was also tested. By experimental results and comprehensively considering the stability of the modification effect and the function requirements, Tween-20 (2.0% v/v) was chosen as the modifying reagents of the first siphon valve and the second siphon valve, and TritonX-100 (2.0% v/v) was chosen for the third siphon valve, which effectively reduces the contact angle and improves the liquid transfer efficiency, leading to further improvement of the rapidity and sensitivity of the centrifugal microfluidic immunoassay system by efficient siphoning and high plasma separation efficiency (99%). Full article

The detection and analysis of circulating tumor cells (CTCs) plays a crucial role in clinical practice. However, the heterogeneity and rarity of CTCs make their capture and separation from peripheral blood very difficult while maintaining their structural integrity and viability. We previously demonstrated the effectiveness of the Magnetic Cell Centrifuge Platform (MCCP), which combined the magnetic-labeling cell separation mechanism with the size-based method. In this paper, a comparison of the effectiveness of different microsieve pore geometries toward MCCP is demonstrated to improve the yield of the target cell capture. Firstly, models of a trapped cell with rectangular and circular pore geometries are presented to compare the contact force using finite element numerical simulations. The device performance is then evaluated with both constant pressure and constant flow rate experimental conditions. In addition, the efficient isolation of magnetically labeled Hela cells with red fluorescent proteins (target cells) from Hela cells with green fluorescent protein (background cells) is validated. The experimental results show that the circular sieves yield 97% purity of the target cells from the sample with a throughput of up to 2 μL/s and 66-fold sample enrichment. This finding will pave the way for the design of a higher efficient MCCP systems. Full article

An efficient and environment-friendly microextraction method, namely, β-cyclodextrin assisted liquid–liquid microextraction, based on solidification of the floating organic droplets method coupled with HPLC is investigated for the sensitive determination of trace neonicotinoid pesticide residues. In this method, β-cyclodextrin is used as a disperser solvent, while 1-octanol is selected as an extraction solvent. β-cyclodextrins was found to decrease interfacial tension and increase the contact area between the organic and water phases with the help of centrifugation. A cloudy solution was rapidly formed and then centrifuged to complete phase separation. Various key parameters influencing extraction efficiency were systematically investigated and optimized; they include salt addition, concentration of β-cyclodextrin, and volume of extraction solvent (1-octanol). Under optimum conditions, good linearity was obtained with coefficient for determination (R²) greater than 0.99. A low limit of detection, high enrichment factor, and good recovery (83 – 132) were achieved. This proves that the proposed method can be applied to determine trace neonicotinoid pesticide residues in natural surface water samples. Full article

Recording of Stribeck-like curves is a common way to study the effect of laser-patterned surfaces on the frictional efficiency. However, solely relying on the coefficient of friction when identifying the lubrication regime and the underlying working principles can be misleading. Consequently, a ball-on-disc tribometer was combined with an electrical resistivity circuit to simultaneously measure Stribeck-like curves and solid-solid contact ratios for polished and laser-patterned samples. Line-like surface patterns with different periodicities were produced by direct laser interference patterning on steel substrates (AISI304). The reference shows a Stribeck-like behavior well correlating with the contact ratios. The behavior deviates for high sliding velocities (high contact ratios) due to a loss of lubricant induced by centrifugal forces pulling the lubricant out of the contact zone. In contrast, the solid–solid contact ratio of the laser-patterned samples is around 80% for all sliding velocities. Those values can be explained by higher contact pressures and the structural depth induced by the surface topography which make a full separation of the surfaces unlikely. Despite those high values for the contact ratio, laser-patterning significantly reduces friction, which can be traced back to a reduced real contact area and the ability to store oil in the contact zone. Full article

We report a new flow control method for centrifugal microfluidic systems; CO₂ is released from on-board stored baking powder upon contact with an ancillary liquid. The elevated pressure generated drives the sample into a dead-end pneumatic chamber sealed by a dissolvable film (DF). This liquid incursion wets and dissolves the DF, thus opening the valve. The activation pressure of the DF valve can be tuned by the geometry of the channel upstream of the DF membrane. Through pneumatic coupling with properly dimensioned disc architecture, we established serial cascading of valves, even at a constant spin rate. Similarly, we demonstrate sequential actuation of valves by dividing the disc into a number of distinct pneumatic chambers (separated by DF membranes). Opening these DFs, typically through arrival of a liquid to that location on a disc, permits pressurization of these chambers. This barrier-based scheme provides robust and strictly ordered valve actuation, which is demonstrated by the automation of a multi-step/multi-reagent DNA-based hybridization assay. Full article