Search Results (19)

The use of alternatives for petroleum-based products is becoming more and more important, especially considering the new and constantly changing geopolitical context, where excessive energy dependence is not desirable. Thus, biodiesel could play an important role in contributing to the implementation of biorefineries, which represent desirable goals in terms of sustainability, green chemistry and the circular economy. However, one challenge related to biodiesel based on vegetable oils is its low oxidative stability, which can alter the properties of these products during storage. To avoid this problem, interesting antioxidants, such as propyl gallate (PG), could be added to biodiesel to allow it to keep its main properties during oxidation. Additionally, monitoring PG content during oxidation is interesting, and the use of voltammetry could be suitable for this purpose. The aim of this work was to assess the effectiveness of PG during cardoon biodiesel oxidation, while monitoring the process through cyclic voltammetry (CV). As a result, it was proven that PG was highly effective, increasing the length of oxidative stability to more than 10 h at low concentrations (600 mg·L⁻¹) and retaining its main properties (viscosity and acidity) during oxidation. Regarding CV, this technique was successfully optimized to determine PG concentration in cardoon biodiesel during oxidation. Full article

The study reports the enzymatic synthesis of bio-based oligoesters and chemo-enzymatic processes for obtaining epoxidized bioplasticizers and biolubricants starting from cardoon seed oil. All of the molecules had M_W below 1000 g mol⁻¹ and were analyzed in terms of marine biodegradation. The data shed light on the effects of the chemical structure, chemical bond lability, thermal behavior, and water solubility on biodegradation. Moreover, the analysis of the biodegradation of the building blocks that constituted the different bio-based products allowed us to distinguish between different chemical and physicochemical factors. These hints are of major importance for the rational eco-design of new benign bio-based products. Overall, the high lability of ester bonds was confirmed, along with the negligible effect of the presence of epoxy rings on triglyceride structures. The biodegradation data clearly indicated that the monomers/building blocks undergo a much slower process of abiotic or biotic transformations, potentially leading to accumulation. Therefore, the simple analysis of the erosion, hydrolysis, or visual/chemical disappearance of the chemical products or plastic is not sufficient, but ecotoxicity studies on the effects of such small molecules are of major importance. The use of natural feedstocks, such as vegetable seed oils and their derivatives, allows the minimization of these risks, because microorganisms have evolved enzymes and metabolic pathways for processing such natural molecules. Full article

Given the current scenario of increasing environmental problems associated with the need for rapid energy transition, this article aimed to investigate the implementation of Cynara cardunculus L. (cardoon), a plant with high environmental performance, as a source of energy resources. This study presented thLife Cycle Assessment of two energy production chains—for the polygeneration of power, heat, and cooling; and to produce biodiesel—fed with cardoon seeds, in addition to comparing these chains with the use of the traditional Italian grids (power and gas) and with the production of diesel based on palm, soybean, and rapeseed. Approximately 49 t of seeds were cultivated and processed, yielding 8.5 t of oil. The system boundaries encompass three main macro-phases, cardoon production, oil extraction, and, depending on the application, energy generation (polygeneration) or transesterification (biodiesel). The models were developed using the software SimaPro V9.3.0.2, and the inventory was based on the database ecoinvent V3.8. The Life Cycle Impact Assessment was performed using the ReCiPe V1.06 method at the midpoint (H) and endpoint (H/A) levels. Crude cardoon oil showed a global warming of 20–55% lower than other vegetable oils (palm, soybean, and rapeseed). In the case of biodiesel production, cardoon-based biodiesel presented a reduction in the impact burden by 12–57% compared to biodiesel based on palm, soybean, and rapeseed. With the use of oil in Polygeneration, a subtle increase in the impact burden was observed, with 13% more impact than the use of Italian power and gas grids. Full article

Cynara cardunculus (Asteraceae family) is a perennial plant native to Mediterranean regions. This plant represents a source of high-value compounds, such as polyphenols and fatty acids that have several industrial applications. However, in vitro plant cell cultures can represent a valid alternative to in-field cultivation and facilitate the extraction of metabolites of commercial interest. Generally, sucrose is the main sugar used for plant cell cultures, but other carbon sources can be considered. Here, we investigated the potential use of alternative organic carbon sources, such as galactose, maltose, glucose, glycerol, fructose, lactose, and starch, for the cultivation of C. cardunculus cells. Moreover, cardoon cells were collected, and an extraction of polyphenols and oils was performed to study the effects of different carbon sources on the production of bioactive molecules. This study provided evidence that cardoon cell growth can be supported by carbon sources other than sucrose. However, the carbon source inducing optimum growth, did not necessarily induce the highest production of high-value compounds. Full article

The lipogenic ability of the yeast Solicoccozyma terricola DBVPG 5870 grown on hydrolyzed lignocellulose obtained from cardoon stalks was evaluated. Data on cell biomass, lipid production, and fatty acid profiles of triacylglycerols obtained in batch and fed-batch experiments were carried out at the laboratory scale in a 5L fermenter, and at two different temperatures (20 and 25 °C) were reported. The higher production of total intracellular lipids (13.81 g/L) was found in the fed-batch experiments carried out at 20 °C. S. terricola exhibited the ability to produce high amounts of triacylglycerol (TAGs) with a characteristic fatty acids profile close to that of palm oil. The TAGs obtained from S. terricola grown on pre-treated lignocellulose could be proposed as a supplementary source of oleochemicals. Indeed, due to the rising prices of fossil fuels and because of the environmental-related issues linked to their employment, the use of TAGs produced by S. terricola grown on lignocellulose could represent a promising option as a supplementary oleochemical, especially for biodiesel production. Full article

Soybean meal is the most important protein source in beef cattle feeding. The research of alternative protein sources to replace soy use, avoiding negative effects on in vivo performance and on the product’s quality, is an important issue. In this context, cardoon represents a non-OGM resilient crop that can be cultivated in marginal lands for extracting its seed oil (utilized for biodiesel and biodegradable bioplastic production) and whose and the residual meal from its seed oil (utilized for biodiesel and biodegradable bioplastic production) could be a suitable by-product for animal feeding, due to its fairly high protein content. The aim of this study was to evaluate the feasibility of using cardoon meal as an innovative protein source during the Limousine bulls’ fattening period. Thirty-two bulls were divided into two groups and fed with a diet containing soybean meal (SG) or partially replacing soybean meal with cardoon meal as a protein source (CG), respectively. The feeding trial lasted about 11 months. Growth performances and meat physical–chemical traits were evaluated. No statistical differences in feed efficiency, average daily gain, or in the main meat quality indicators, as well as in fatty acid profiles were found among the groups. Therefore, cardoon meal could be considered as an alternative to soybean meal in fattening Limousine bulls in order to enhance the sustainability of the farming system. Full article

Cynara cardunculus L. is a plant of the Mediterranean basin, known since antiquity as a food and for its therapeutic properties. The needs of the 21st century for the utilization of agricultural waste has led to the study of the seed oil of a Greek cultivar of Cynaracardunculus (GCCC) as potential nutritional oil, as large amounts of cardoon seeds are discarded. The sterol and fatty acid profile of cold-pressed seed oil was examined by gas chromatography-mass spectrometry GC-MS and compared with that of solvent extraction. Total phenolic content was determined and compared with well-known and widely appreciated edible vegetable oils; while, additionally, the total lignan content and nutritional value of cold-pressed oil revealed it as a potential dietary candidate. Furthermore, the seedcake (residue of cold-pressed oil extraction) has been studied exerting it as a good source of phenolics. Both GCCC oil and seedcake were tested for their antioxidant and enzyme inhibitory activities exhibiting higher activity compared to the sesame, flaxseed and extra virgin olive oils. According to the results, Cynara seed oil was shown to be a rich source of ω-6/-9 fatty acids and phenolics, highlighting, indicating that it could be a promising health-promoting vegetable oil, while the seedcake was revealed as a rich source of bioactive compounds. Full article

Hydrolysis and butanolysis of lignocellulosic biomass are efficient routes to produce two valuable bio-based platform chemicals, levulinic acid and n-butyl levulinate, which find increasing applications in the field of biofuels and for the synthesis of intermediates for chemical and pharmaceutical industries, food additives, surfactants, solvents and polymers. In this research, the acid-catalyzed hydrolysis of the waste residue of Cynara cardunculus L. (cardoon), remaining after seed removal for oil exploitation, was investigated. The cardoon residue was employed as-received and after a steam-explosion treatment which causes an enrichment in cellulose. The effects of the main reaction parameters, such as catalyst type and loading, reaction time, temperature and heating methodology, on the hydrolysis process were assessed. Levulinic acid molar yields up to about 50 mol % with levulinic acid concentrations of 62.1 g/L were reached. Moreover, the one-pot butanolysis of the steam-exploded cardoon with the bio-alcohol n-butanol was investigated, demonstrating the direct production of n-butyl levulinate with good yield, up to 42.5 mol %. These results demonstrate that such residual biomass represent a promising feedstock for the sustainable production of levulinic acid and n-butyl levulinate, opening the way to the complete exploitation of this crop. Full article

Today one of the most interesting ways to produce biodiesel is based on the use of oleaginous microorganisms, which can accumulate microbial oil with a composition similar to vegetable oils. In this paper, we present a thermo-chemical numerical model of the yeast biodiesel production process, considering cardoon stalks as raw material. The simulation is performed subdividing the process into the following sections: steam explosion pre-treatment, enzymatic hydrolysis, lipid production, lipid extraction, and alkali-catalyzed transesterification. Numerical results show that 406.4 t of biodiesel can be produced starting from 10,000 t of lignocellulosic biomass. An economic analysis indicates a biodiesel production cost of 12.8 USD/kg, thus suggesting the need to increase the capacity plant and the lipid yield to make the project economically attractive. In this regard, a sensitivity analysis is also performed considering an ideal lipid yield of 22% and 100,000 t of lignocellulosic biomass. The biodiesel production costs related to these new scenarios are 7.88 and 5.91 USD/kg, respectively. The large capacity plant combined with a great lipid yield in the fermentation stage shows a biodiesel production cost of 3.63 USD/kg making the product competitive on the current market of biofuels by microbial oil. Full article

The focus of this study is to analyze the energy flows related to cardoon oil processing based on the estimated electricity consumption. It was carried out in a prototype oilseed extraction plant located in Umbria Region, Italy. This installation consists of the following processes: seed weight-storage, transportation, sifting, milling, filtering, and oil storage. Approximately 49,000 kg dry basis (DB) of cardoon seeds (Cynara cardunculus) were processed. Cardoon seeds were chosen as a feedstock due to their sustainability characteristics. Before processing, samples of raw material were analyzed in terms of moisture, oil content, impurity, density, and lower heating value. The input–output flows were measured in terms of weight and energy consumption. Its data were used to perform the mass-energy balance and estimate the efficiency of processes. About 8505 kg of filtered oil was obtained from raw material. The efficiency of the process chain was 84.42% concerning the oil content in the seeds. For the same period, the plant required a total of 17,556 MJ of electric energy from the grid for its operation. 321,507 MJ were obtained from oil and 688,875 MJ from oilcake. About 5.48% of the energy produced by oil extraction was virtually used in the process. Full article

Cultivated cardoon (Cynara cardunculus var. altilis) has long been used as a food and medicine remedy and nowadays is considered a functional food. Its leaf bioactive compounds are mostly represented by chlorogenic acids and coumaroyl derivatives, known for their nutritional value and bioactivity. Having antioxidant and hepatoprotective properties, these molecules are used for medicinal purposes. Apart from the phenolic compounds in green tissues, cultivated cardoon is also used for the seed oil, having a composition suitable for the human diet, but also valuable as feedstock for the production of biofuel and biodegradable bioplastics. Given the wide spectrum of valuable cardoon molecules and their numerous industrial applications, a detailed characterization of different organs and tissues for their metabolic profiles as well as an extensive transcriptional analysis of associated key biosynthetic genes were performed to provide a deeper insight into metabolites biosynthesis and accumulation sites. This study aimed to provide a comprehensive analysis of the phenylpropanoids profile through UHPLC-Q-Orbitrap HRMS analysis, of fatty acids content through GC-MS analysis, along with quantitative transcriptional analyses by qRT-PCR of hydroxycinnamoyl-quinate transferase (HQT), stearic acid desaturase (SAD), and fatty acid desaturase (FAD) genes in seeds, hypocotyls, cotyledons and leaves of the cardoon genotypes “Spagnolo”, “Bianco Avorio”, and “Gigante”. Both oil yield and total phenols accumulation in all the tissues and organs indicated higher production in “Bianco Avorio” and “Spagnolo” than in “Gigante”. Antioxidant activity evaluation by DPPH, ABTS, and FRAP assays mirrored total phenols content. Overall, this study provides a detailed analysis of tissue composition of cardoon, enabling to elucidate value-added product accumulation and distribution during plant development and hence contributing to better address and optimize the sustainable use of this natural resource. Besides, our metabolic and transcriptional screening could be useful to guide the selection of superior genotypes. Full article

Cynara cardunculus is a perennial plant that adapts well to Mediterranean climate conditions. The possibility of cultivating C. cardunculus with low or zero input and in low productivity or marginal lands makes it one of the most promising crops for bioenergy production in the Mediterranean areas. The objective of the research was to study the effects of two marginal and contrasting southern Italian environments (plain, 42 m a.s.l. and hilly area, 419 m a.s.l.) during a three-year period on biomass, seed, energy yield, and oil composition of two genotypes of C. cardunculus (cultivated cardoon and wild cardoon). When compared to the plain, plants that were grown in the hills gave higher biomass yield (10.9 vs. 9.7 t DM ha⁻¹ year⁻¹), higher seed yield (0.46 vs. 0.44 t DM ha⁻¹ year⁻¹) and, consequently, higher total energy yield (190 vs. 172 GJ ha⁻¹ year⁻¹), attributable to higher average annual rainfall (680 vs. 565 mm year⁻¹). The season, although only evaluated for three years (short cycle), showed a different effect based on the genotype, highlighting a greater over-time production constancy of wild cardoon (though less yielding) as compared to cultivated cardoon. Oil yield and composition were only slightly affected by environment and genotype. Overall, the results of this research suggest using C. cardunculus in marginal hilly areas, where, in addition to the production of bioenergy, it may represent a good chance to fight erosion and improve soil fertility, without competing with food crops. Full article

The sustainable production of renewable energy is a key topic on the European community’s agenda in the next decades. The use of residuals from agriculture could not be enough to meet the growing demand for energy, and the contribution of vegetable oil to biodiesel production may be important. Moreover, vegetable oil can surrogate petroleum products in many cases, as in cosmetics, biopolymers, or lubricants production. However, the cultivation of oil crops for the mere production of industrial oil would arise concerns on competition for land use between food and non-food crops. Additionally, the economic sustainability is not always guaranteed, since the mechanical harvesting, in some cases, is still far from acceptable. Therefore, it is difficult to plan the future strategy on bioproducts production from oil crops if the actual feasibility to harvest the seeds is still almost unknown. With the present review, the authors aim to provide a comprehensive overview on the state of the art of mechanical harvesting in seven herbaceous oil crops, namely: sunflower (Heliantus annuus L.), canola (Brassica napus L.), cardoon (Cynara cardunculus L.), camelina (Camelina sativa (L.) Crantz), safflower (Carthamus tinctorius L.), crambe (Crambe abyssinica R. E. Fr.), and castor bean (Ricinus communis L.). The review underlines that the mechanical harvesting of sunflower, canola and cardoon seeds is performed relying on specific devices that perform effectively with a minimum seed loss. Crambe and safflower seeds can be harvested through a combine harvester equipped with a header for cereals. On the other hand, camelina and castor crops still lack the reliable implementation on combine harvesters. Some attempts have been performed to harvest camelina and castor while using a cereal header and a maize header, respectively, but the actual effectiveness of both strategies is still unknown. Full article

The Food and Agriculture Organization’s previsions show that by 2050 the world’s population will reach 9.6 billion people, and the request for a high value protein source will increase as well. Poultry can guarantee high value protein for humans, even in the poorest regions of the world. Hence, efficient poultry production is needed, matching with sustainable development. The residual meal from cardoon seed oil (used for biodiesel and biodegradable bioplastic production) is suitable for animal feeding due to its protein content. The aim of this preliminary study was to test for a possible use of cardoon meal as a protein source in a poultry diet during the finishing period. Forty-five Kabir chickens were divided into three groups and fed three diets in which soybean meal (control) was partially (16%) or completely replaced with cardoon meal as a protein source (treated groups). In vivo performances, animal welfare, dressing out and meat color were evaluated. No statistical differences in feed efficiency, dressing out, nor in meat quality were found among groups. Moreover, birds that were fed cardoon meal showed lower perivisceral fat. Therefore, cardoon meal could be considered as an alternative for soybean meal in the finishing period in poultry feeding. Full article

The use of non-edible vegetable oils to produce oleochemicals has been attracting more attention in recent years. Cardoon seed oil, derived from the Cynara C. plant, growing in marginal and contaminated lands, represents a non-edible alternative to soybean oil to obtain plasticizers through epoxidation reaction. The use of hydrogen peroxide as oxidant and in the presence of a heterogeneous catalyst allows overcoming the limits of epoxidation with peracids. γ-alumina has been shown to have an active catalyst epoxidation reaction with hydrogen peroxide, mainly using acetonitrile as solvent. However, the use of acetonitrile as solvent is widely debated due to its hazardous character and health issues. For these reasons, the influence of solvent on the reaction was studied in this work to find a more environmentally friendly and stable solvent. The study showed that the epoxidation reaction takes place also in the absence of solvent although with lower selectivity. The type of solvent influences both the epoxidation and decomposition reactions of hydrogen peroxide. γ-valerolactone was found to be the most promising solvent for cardoon oil epoxidation reaction. This finding represents a noteworthy novelty in the field of epoxidation of vegetable oils with hydrogen peroxide, opening the way to greener and cleaner process. Finally, an optimization study showed that the most effective molar ratio between hydrogen peroxide and double bonds for better selectivity was 4.5 and the need to use the highest possible initial concentration of hydrogen peroxide (approximately 60 wt. %). Full article