Search Results (618)

White Striping (WS) is a macroscopic defect of the pectoralis major muscle, characterized by distinct white striations that impair meat acceptability and commercial value. It is a phenotype with polygenic inheritance, controlled by several QTLs and genes associated with muscle repair and metabolism. Beyond genetic factors, phenotypic manifestation is strongly modulated by the environment. This review integrates research on genetic predispositions and modulating factors to provide a holistic overview of WS in broilers. The defect predominantly affects heavier birds with high breast yield and elevated ultimate breast pH. LRSAM1 gene, on chromosome GGA17, is identified as a putative candidate gene as its expression co-localizes with the phenotypic QTL. Chromosome GGA5 has recently been identified as the primary genomic region of interest hosting a cluster of specific markers. Research on dietary strategies has extensively explored the manipulation of feed formulations, especially of amino acids. While results for some nutrients like methionine remain conflicting, restricting lysine during the growth phase could be an effective dietary intervention for reducing WS severity. Management offers the most practical short-term solutions, whereas selective breeding enables meaningful and permanent progress across generations, given the moderate heritability of many quality-related traits. Effective mitigation requires an integrated approach combining welfare, environmental control, and precision feeding throughout the production cycle, while acknowledging trade-offs with productivity. To meet evolving consumer expectations, the industry must embrace practices that are simultaneously scientifically rigorous, ethically responsible, and environmentally sustainable. Full article

Wuhua Yellow Chicken (WYC) is a Guangdong heritage breed known for its characteristic “three yellow” phenotype and distinctive meat flavour. Despite its commercial importance, data on muscle flavour chemistry remain scarce. In this study, 180 one-day-old chicks (90 cocks, 90 hens, 18 replicates of 5 chickens per sex) were raised to 20 weeks under cage conditions, after which slaughter traits, meat physicochemical indices, proximate composition, amino acid and fatty acid profiles, and volatile compounds were measured. Cocks were heavier and had higher eviscerated yields and leg muscle percentages, whereas hens accumulated more abdominal fat (6.47–0.46%, p < 0.01). Shear force was greater in cock breast muscle (2.86–2.13 kg·f, p < 0.01), indicating firmer texture. Cock breast muscle contained more crude protein (26.89%) and less crude fat. Amino acid totals were identical between sexes (21.10 g/100 g), with all six essential amino acids surpassing FAO/WHO reference values; lysine scored highest (168%). Unsaturated fatty acid proportions were 63.33% (cocks) and 66.64% (hens), with PUFA/SFA ratios of 61.95% and 53.60%, respectively. Gas chromatography-mass spectrometry identified 10 volatile compounds in cocks and 14 in hens; aldehydes dominated in both, with hexanal alone accounting for over 50%. Hen muscle contained a richer volatile profile, including additional ketone and ester compounds. These data collectively confirm that WYC is nutritionally dense, organoleptically appealing, and well-suited for further breed promotion. Full article

Body weight variation within a breed may be associated with meat flavor in chickens, but its relationship with flavor-related precursor composition across developmental stages remains unclear. Here, integrated lipidomics and metabolomics were applied to compare breast muscle from Beijing-You chickens sampled from the same cohort at 90, 110, 130, and 150 d in a stage-wise design. At each stage, higher-body-weight (HBW) and lower-body-weight (LBW) groups were independently defined from the upper and lower tails of the body weight distribution at that age. A total of 440, 259, 161, and 324 differential lipids, as well as 491, 257, 291, and 402 differential metabolites, were identified at the four stages, respectively. However, only 23 lipids and 3 metabolites were shared across all stages, indicating that metabolic differences between the HBW and LBW groups varied markedly across developmental stages. Differential lipids were mainly distributed among phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine, while glycerophospholipid metabolism was consistently identified in both lipidomic and metabolomic analyses. Notably, a key transition was observed between 110 and 130 d, during which the predominant direction of PUFA-like differential lipids shifted from HBW to LBW predominance. Representative differential metabolites included N-acetyl-L-methionine, N-methyl-L-glutamic acid, and γ-glutamyl-5-hydroxytryptophan, suggesting alterations in amino acid- and peptide-related metabolism. Overall, these findings provide insight into stage-dependent variation in flavor-related precursor composition within a breed across developmental stages. However, their direct contribution to flavor remains to be validated. Full article

Local chicken breeds are increasingly being reconsidered as a means to produce distinctive meat in non-conventional systems while also supporting the conservation of endangered genetic resources. This study compared Banat Naked Neck (BNN) and Svrljig hen (SH), two Serbian indigenous breeds, reared under identical pasture-based conditions and slaughtered at 12 or 14 weeks. Carcass traits, including linear measurements and carcass composition, were evaluated in 40 males (10 per breed per age), while breast and thigh-with-drumstick meat quality (proximate composition and fatty acid profile) was analysed in 80 samples (10 per tissue per group). Data were analysed using two-way ANOVA, and multivariate patterns were explored using PCA and residual Spearman correlation analysis. BNN and SH showed similar slaughter weights, whereas slaughter at 14 weeks increased carcass conformation measures and conformation indices (p < 0.05). Breed differences were most evident in carcass part distribution and tissue partitioning within cuts: BNN had a higher breast proportion and breast meat yield, whereas SH meat was leaner and thigh with drumstick meat showed higher Σn − 3 and a more favourable Σn − 6/Σn − 3 ratio. PCA indicated clearer breed separation in thigh meat than in breast meat, consistent with the univariate lipid results, and residual correlations highlighted expected allocation trade-offs among carcass and cut components. Overall, slaughter at 14 weeks improved carcass value, and both breeds offer complementary traits for market-oriented conservation through use. Full article

Estimating dietary requirements is resource-intensive and often excludes common commercial practices, such as the use of feed additives (FA). The objective of this study was to evaluate whether a factorial–mechanistic growth model-derived feeding program would improve performance and economic efficiency compared to the 1994 NRC recommendations for commercial turkey toms. This applied study used 5248 Nicholas Select toms (day 0 to 140) in a 2 × 2 factorial design comparing two nutrition programs (growth MODEL versus 1994 NRC recommendations) with or without feed additives (FA). Hatch-mate toms were placed across two research farms, with eight replicate pens per treatment at each farm. Growth and efficiency measurements were collected throughout the trial, and carcass yield was measured at 140 days. Data were analyzed using mixed linear models with nutritional model, FA, phase, location, and all interactions as fixed effects. The main effect of the nutritional model was different for body weight, feed intake, feed conversion ratio, and lysine to gain (p < 0.030), with the MODEL program demonstrating favorable outcomes except for body weight through the first 42 days, which was the only variable impacted by FA (p = 0.014). Nutritional program by FA interactions affected breast meat yield, feed conversion ratio, and lysine conversion, with FA improving outcomes for toms fed the MODEL feeding program but not the NRC feeding program. These findings support the use of factorial growth models over traditional NRC feeding programs. Full article

Canola meal, with environmentally friendly attributes, lower cost, and previous studies, is an interesting proteic source to partially replace the soybean meal for poultry nutrition. For that, in this work we aimed to investigate canola meal as a partial replacement of soybean meal in finishing poultry diets (21 to 49 days) on the productive performance, also including the impact on the quality, nutritional attributes and antioxidative status of valuable cuts of meat. Ninety-six 21-day-old chickens were assigned to four experimental diets (24/diet), with increasing doses of canola meal (CM 0, 2.5, 5 and 10%). Daily consumption, weekly live weight and post mortem carcass weight and yield were determined. At 24 h post mortem, pH, color (CIE L, a*, b*) and drip loss were measured in the breast, drumstick and thigh cuts. Fatty acid composition and health lipid indexes were also determined in the fresh cuts. The oxidative status of lipids and proteins, polyphenol and flavonoids content in fresh and in stored (7 days-display at 4–6 °C) in vacuum packaged cuts were determined. Including CM, up to 10%, the feed intake and growth of birds was not affected (p = 0.74 and p = 0.87 respectively). In meat, CM significantly decreased the drip loss (p < 0.05), the pH in breast and thigh (p = 0.01 and p = 0.05 respectively), a lower L and b in thigh and increased PUFAs in more oxidative cuts, with a strong interaction between dose and muscle type. There was no effect on lipid oxidation while carbonyls decrease at a 2.5% dose in fresh and stored cuts but there is an increase with higher ones. Flavonoids raise the maximum deposition in meat at 5% CM. In conclusion, CM can be included in finishing poultry diets, but high doses must to be adequately managed if performance and quality of meat criteria are considered together. Full article

Black-boned chicken is a native and valuable breed that is very important in the meat products of China. However, the molecular mechanisms underlying differences in muscle flavor between sexes remain unclear. In this study, 360-day-old male (BM, n = 6) and female (BF, n = 6) Xichuan black-boned chickens were used to screen differential lipids and differential flavor compounds in breast muscle tissue by lipidomics and flavoromics. This was followed by multivariate statistical analysis, functional enrichment and correlation network analysis of the differential lipids and flavor compounds obtained. Lipidomics identified 419 differential lipids associated with BM vs. BF, which were mainly enriched in glycerolipid metabolism and metabolic pathways. Flavoromics analysis identified 61 differential flavor compounds, and enrichment analysis showed that the terpenoid backbone biosynthesis pathway may be correlated with chicken muscle flavor formation. Correlation analysis revealed that triglyceride-type lipid molecules were closely related to the flavor compound 3-ethyl-2-methylheptane. These findings provide novel insights into the sex-related differences in the meat quality of Xichuan black-boned chickens, offering important data for their recognition and evaluation. Full article

Plant polysaccharides, such as Astragalus polysaccharide (APS) and Glycyrrhiza polysaccharide (GPS), hold potential as feed additives, yet their individual and synergistic effects on squab meat quality remain unclear. In this study, 192 healthy, 15-day-old, early-weaned Silver King squabs were assigned to one of four dietary treatments for 28 days: a control group (CK), an APS group, a GPS group, and a combined APS + GPS group (AG). Slaughter traits, organ indices, liver antioxidant capacity, and meat quality were evaluated across the four groups. Results indicated that supplementation with APS, GPS, and AG enhanced several slaughter traits compared to CK, including live weight, carcass weight, full-eviscerated weight, half-eviscerated weight, and leg muscle weight. GPS and AG supplementation improved color parameters in both breast and leg muscles, with AG showing the most favorable tenderness-related outcomes. Additionally, AG supplementation enhanced liver antioxidant capacity, as evidenced by increased total antioxidant capacity and superoxide dismutase activity. Given AG’s superior overall performance, the ileal metabolomics analysis focused on comparing CK and AG. Metabolomics data revealed clear group separation and significant changes in amino acid-related pathways. In summary, while APS and GPS individually improved certain traits, their combined supplementation yielded the most favorable results, likely through enhanced antioxidant capacity and altered ileal amino acid metabolism. Full article

The present work was designed to investigate the impacts of tartary buckwheat flavonoids (TBF) on the growth performance and physiological metabolism of Liangshan Yanying chickens. A total of 144 healthy 4-week-old Liangshan Yanying chickens of uniform body weight were randomly divided into four groups. Each group consisted of six replicates with six chickens per replicate. The control group was fed a basal diet, whereas the treatment groups received the same basal formulation supplemented with TBF at concentrations of 20, 40, and 60 mg/kg, respectively. The entire feeding trial lasted for 10 consecutive weeks. Growth performance, serum parameters, bone quality, slaughter traits, and hepatic lipid metabolism were determined and statistically analyzed. Results showed that dietary TBF supplementation had no significant impact on the overall growth performance (p > 0.05); however, final body weight and average daily weight gain displayed a positive linear trend in response to increasing TBF levels (0.05 < p < 0.1). For serum parameters, TBF supplementation significantly increased total antioxidant capacity (p < 0.05) and decreased malondialdehyde content (p < 0.05) in a linear manner. Specifically, compared with the control group, the 60 mg/kg TBF group increased T-AOC by approximately 64.6% and reduced MDA by approximately 67.9%, demonstrating a robust antioxidant effect. A linear increase in high-density lipoprotein cholesterol levels approaching significance (p = 0.055) was also observed. A significant quadratic regulatory effect of TBF was observed on serum glucose concentrations (p < 0.05), whereby the 60 mg/kg TBF dose reduced serum glucose by 15.6% relative to the control (p < 0.05), reflecting a robust hypoglycemic effect. Regarding bone quality, supplementation with 20 and 60 mg/kg TBF significantly elevated tibial phosphorus content relative to the 0 mg/kg TBF group (p < 0.05). Regression analysis showed that increasing dietary TBF levels linearly elevated tibial calcium content (p < 0.05) and resulted in linear increasing trends in tibial ash content, tibial phosphorus content, femur ash content, and femur calcium content (0.05 < p < 0.1). Concerning slaughter performance, dietary TBF inclusion resulted in a significant linear rise in breast muscle percentage (p < 0.05). At the molecular level, TBF upregulated the mRNA expression of hepatic AMPKα1 and CPT1, while concurrently downregulating the expression of FAS and ACC (p < 0.05). Collectively, dietary TBF supplementation in Liangshan Yanying chickens effectively improved antioxidant capacity, promoted tibial calcium and phosphorus deposition, regulated hepatic fatty acid oxidation and synthesis via AMPK-related genes, and enhanced lean meat deposition, with no adverse effects on growth performance under the experimental conditions. Full article

Consumer preference for clean-label products is driving interest in natural antioxidants and antimicrobials that can replace synthetic preservatives. Ziziphus mauritiana (sidr), a resilient desert tree native to the arid Gulf region, has being tested as a multifunctional bio-preservative. This study evaluated the extraction yield, total phenolic content (TPC), total flavonoid content (TFC), antioxidant and antimicrobial activities, and poultry meat-preserving potential of Z. mauritiana. Methanol and ethanol produced the highest extract recoveries, with bark exhibiting the maximum extraction yield of up to 10.7 mg/100 g. Fruits demonstrated the highest total phenolic content (TPC) of around 175 mg gallic acid equivalents per gram (GAE/g) and total flavonoid content (TFC) of around 7.4 mg catechin equivalents per gram (CE/g), followed by leaves and bark. The antioxidant activity was significantly correlated with the concentration of phenolic compounds in the fruit extracts, which exhibited DPPH inhibition exceeding 60% in the majority of instances. The RP-HPLC investigation revealed a diverse polyphenolic profile, predominantly featuring gallic acid (up to 8.77 mg/g in leaves), catechin (6.30 mg/g in fruits), catechol, and caffeic acid. Leaf extracts showed 24 mm inhibitory zones against E. coli and Y. enterocolitica, while bark and fruit were not very effective. Adding ethanolic leaf extract (0–1%) to chicken breast meat reduced microbial degradation during chilled storage at 4 °C. At day 15, total aerobic counts reached only 5.34 log CFU/g with 1% extract compared with 8.53 log CFU/g in the control. Similar suppression was found for yeasts and molds, while challenge tests showed >3-log reductions in C. jejuni and Salmonella senftenberg. Sensory evaluation confirmed no detrimental effects on color, odor, flavor, or texture. Overall, Z. mauritiana was a valuable, renewable source of phenolic antioxidants and antimicrobial agents and showed strong promise as a natural preservative capable of improving the safety and shelf life of poultry meat in clean-label applications. Full article

Chickens are a primary source of protein in the human diet, with demand increasing annually. However, research on genes that promote chicken meat development remains relatively limited. Therefore, in this study, breast muscle samples (pectoralis major, n = 10 per group) from chickens at five developmental stages (D1, D35, D70, D105, and D140) were selected to investigate genetic-level changes and identify additional genes influencing chicken meat development. Differential expression analysis between adjacent stages (|FC| > 1.5, p < 0.05) revealed 42 differentially expressed genes (DEGs) shared across four comparisons, primarily enriched in muscle development pathways such as focal adhesion, the regulation of the actin cytoskeleton, and Wnt signaling. Weighted gene coexpression network analysis (WGCNA) revealed that four modules were significantly correlated with body weight and breast muscle weight phenotypes. By integrating the hub genes of the four modules and the DEGs, we identified key genes, including MEGF10, MYOM2, TM4SF1, HNMT, NR4A3, and Wnt5a. Furthermore, we conducted a comparative analysis of key gene expression trends across commercial broilers (CBs) and Beijing You (BJY) chickens. MYOM2 and Wnt5a exhibited distinct expression patterns during the early developmental stage of Xianju (XJ) chickens, suggesting that these genes may be critical factors distinguishing XJ chicken breast muscle development from that of other breeds. In all three chicken breeds analyzed, the expression levels of HNMT and MEGF10 gradually decreased with increasing age, indicating that their functions are universal in poultry muscle development. In summary, our findings revealed key regulatory genes that influence breast muscle development, offering candidate targets for marker-assisted selection in poultry breeding programs. Full article

Xichuan black-boned chicken is a premium Chinese local breed in Xichuan County, Henan Province, China. However, the flavor characteristics of Xichuan black-boned chicken meat have not been systematically studied. Lipidomics and flavoromics approaches were used to analyze DLMs (differential lipid molecules) and DFCs (differential flavor compounds) in breast muscle (BM, n = 6) and leg muscle (LM, n = 6) of black-boned chicken, to reveal molecular mechanisms affecting meat quality in chicken. Lipidomics analysis reveals that 354 differential lipids are the differential abundance between the two groups, of which 33 are up-regulated and 321 are down-regulated in the BM group. These differential lipids were mostly enriched in glycerolipid metabolism, glycerophospholipid metabolism, and metabolic pathways. Flavoromics results demonstrate that there are 70 differential flavors between the two groups. Of these flavors, 59 are down-regulated and 11 are up-regulated in the BM group. These differential flavor compounds are mainly enriched in insect hormone biosynthesis and terpenoid backbone biosynthesis. Integrated lipidomics and flavoromics analysis shows that TG-type lipids and dodecanenitrile flavors may be the major related pairs. These findings not only enhance the understanding of the mechanism of chicken meat flavor formation but also provide novel perspectives for the improvement of meat quality. Full article

Dietary metabolizable energy (ME) and crude protein (CP) are key determinants of production efficiency in geese; however, their combined effects during the rapid growth phase are not well defined. A total of 240 male goslings were assigned to four treatments in a 2 × 2 factorial arrangement, with six replicates per treatment and 10 birds per replicate. We used a 2 × 2 factorial design to evaluate two ME levels (11.20 vs. 11.65 MJ/kg) and two CP levels (16% vs. 14%) in goslings from 35 to 63 days of age. Growth performance, carcass traits, meat quality, serum biochemical indices, and instrumental taste attributes were measured. Increasing ME increased body weight at day 63 and average daily gain (p < 0.05), whereas average daily feed intake and feed-to-gain ratio were not affected. Most carcass traits were unchanged; however, leg muscle percentage differed between ME levels (p < 0.01) and was higher in the 11.20 MJ/kg group. Meat color responses were muscle- and time-dependent: breast b* at 45 min postmortem was affected by ME and CP (p < 0.001), and leg color traits at 45 min exhibited significant ME × CP interactions (p < 0.05). Postmortem pH, water-holding capacity, and shear force were largely unaffected by dietary treatments. Serum glucose showed a significant ME × CP interaction (p = 0.001), and triglyceride concentration was influenced by both ME and CP (p < 0.01), with lower values observed at higher ME and lower CP. Instrumental taste attributes did not differ among treatments (p > 0.05). In conclusion, modest changes in dietary ME and CP modulated growth and selected carcass, color, and metabolic traits without compromising key technological meat-quality parameters. These results indicate that, during 35–63 days of age, the higher-ME diet (11.65 MJ/kg) combined with a moderate CP reduction to 14% can be considered a feasible formulation option under the conditions of this study. Full article

The present study evaluated the effects of dietary inclusion of Hermetia illucens (HI) and Tenebrio molitor (TM) larvae meals at 2% and 4% on growth performance, carcass yield, meat quality, blood parameters, and intestinal morphometry in broiler chickens. A total of 1750 one-day-old male Ross 308 broilers were assigned to five dietary treatments: a Control diet, or diets containing 2% or 4% of HI meal (HI2, HI4) or TM meal (TM2, TM4). Growth performance, feed intake (FI), feed conversation ratio (FCR), and mortality were unaffected by dietary treatments. Breast yield increased significantly in insect-fed groups (29.2–29.9%) compared with Control (27.6%). Birds fed HI4 exhibited lower breast pH (5.77 vs. 5.89) and increased cooking loss (29.2% vs. 27.3%), suggesting reduced WHC within acceptable ranges. Thigh meat showed dose-dependent lipid accumulation in insect-fed birds. Serum total cholesterol increased in TM-fed birds with elevated HDL-cholesterol, while LDL-cholesterol remained unaffected. TM meal specifically induced shorter ileal length compared with Control and HI-fed groups. Overall, both full-fat insect meals can be safely incorporated at low inclusion levels without adverse effects on broiler growth, health, or carcass quality. Full article

Meat quality characteristics are important economic traits of ducks. To identify the molecular bases of these traits, we performed an integrated multi-omics analysis (metabolomics, transcriptomics, and miRNAomics) that compared the breast muscle of 300-day-old Liancheng white duck (LD), which is a lean-type breed prized for its soup flavor, and traditional meat duck Cherry Valley duck (CD), which is a fast-growing fat-type breed used for roasting. The results show that LD had higher levels of amino and bile acids, while CD had higher levels of carbohydrates. Integration analysis revealed key breed-specific molecular signatures. In LD, upregulation of the amino acid transporters SLC7A6 and SLC6A9 related to amino acid transport was consistent with elevated intramuscular amino acids. For carbohydrate metabolism, SOCS3—a well-established negative regulator of glucose uptake in mammalian skeletal muscle—was significantly upregulated in LD, consistent with their lower intramuscular carbohydrate levels. SLC6A9 and SOCS3 were predicted to be negatively regulated by oan-miR-1386. In LD, upregulation of the bile acid biosynthesis gene CH25H paralleled the higher bile acid content, suggesting complex, tissue-specific regulation of these pathways. This integrated analysis provides a resource for candidate genes, miRNAs, and metabolic pathways underlying breed-specific meat quality traits in ducks. The findings generate testable hypotheses for future functional studies and offer potential molecular targets for breeding strategies aimed at improving poultry meat quality. Full article