Design and assessment activities associated with a biopharmaceutical process are performed at different levels of detail, based on the stage of development that the product is in. Preliminary “back-of-the envelope” assessments are performed early in...
In Mexico, cheese whey (CW) is commonly treated as a dairy wastewater despite its high lactose and nutrient content. This study evaluated cheese whey (CW) and ultrafiltered cheese whey (UF-CW) as low-cost substrates for the cultivation of the probiot...
Bioprocess simulation is a powerful tool for leveraging circular economy principles in the analysis of large-scale bioprocesses, enhancing decision-making for efficient and sustainable production. By simulating different process scenarios, researcher...
The goal of this research was to evaluate the impact of different bioprocessing techniques on improved bioaccessibility of phenolics from spelt seeds. Despite the negative influence of gastrointestinal digestion, fermentation of germinated seeds sign...
A current concern is the increase in greenhouse gas emissions, mainly CO2, with anthropogenic sources being the main contributors. Microalgae have greater capacity than terrestrial plants to capture CO2, with this being an attraction for using them a...
Knowledge of the mathematical models of the fermentation processes is indispensable for their simulation and optimization and for the design and synthesis of the applicable control systems. The paper focuses on determining a dynamic mathematical mode...
Microalgae have a high capacity to capture CO2. Additionally, biomass contains lipids that can be used to produce biofuels, biolubricants, and other compounds of commercial interest. This study analyzed various scenarios for microalgae lipid producti...
The purpose of this work was to develop a problem-solving approach and a simulation tool that is useful for the specification of wastewater treatment process equipment design parameters. The proposition of using an artificial neural network (ANN) num...
The design and implementation of a simulator, as a real-time application, for a complex process from the biological treatment stage of a wastewater treatment plant (WWTP), is addressed. More precisely, this emulator was achieved as a software tool th...
The dawn of the new millennium saw a trend towards the dedicated use of microfluidic devices for process intensification in biotechnology. As the last decade went by, it became evident that this pattern was not a short-lived fad, since the deliverabl...
Large-scale bioprocesses are increasing globally to cater to the larger market demands for biological products. As fermenter volumes increase, the efficiency of mixing decreases, and environmental gradients become more pronounced compared to smaller...
Volatile fatty acid (VFA) rich streams from fermentation of organic residuals and wastewater are suitable feedstocks for mixed microbial culture (MMC) Polyhydroxyalkanoate (PHA) production. However, many such streams have low total VFA concentration...
Integrated or holistic process models may serve as the engine of a digital asset in a multistep-process digital twin. Concatenated individual-unit operation models are effective at propagating errors over an entire process, but are nonetheless limite...
Enhancing the biochemical supply chain towards sustainable development requires more efforts to boost technology innovation at early design phases and avoid delays in industrial biotechnology growth. Such a transformation requires a comprehensive ste...
Vaccines are integral to human life to protect them from life-threatening diseases. However, conventional vaccines often suffer limitations like inefficiency, safety concerns, unavailability for non-culturable microbes, and genetic variability among...
Regenerative therapies for the treatment of peripheral nerve and spinal cord injuries can require hundreds of millions of autologous cells. Current treatments involve the harvest of Schwann cells (SCs) from nerves; however, this is an invasive proced...
Industrial-sized fermenters differ from the laboratory environment in which bioprocess development initially took place. One of the issues that can lead to reduced productivity on a large scale or even early termination of the process is the presence...
As the world recognizes the need for more sustainable practices, greater effort has been put into improving the economic viability of bioprocesses. Fields like metabolic engineering explore strategies to improve the production of bioproducts of indus...
Lignocellulose is an abundant and renewable resource that holds great promise for sustainable bioprocessing. However, unpretreated lignocellulose is recalcitrant to direct utilization by most microbes. Current methods to overcome this barrier include...
This manuscript provides a comprehensive review of the enzymatic hydrolysis of lignocellulosic biomass, emphasizing how chemical composition, structural features, inhibitory compounds, and process configurations collectively influence the conversion...
The aim of the study was to determine whether the partial replacement of wheat flour with bioprocessed spelt flour contributes to a higher bioaccessibility of the antioxidants in bread. The results showed that the type and amount of bioprocessed spel...
An innovative and integrated scheme that encompasses two well-established waste treatment technologies, the aerobic biological degradation of organic matter bioprocess via membranes and anaerobic digestion, was demonstrated as a zero-waste approach t...
Background: Flux analyses, such as Metabolic Flux Analysis (MFA), Flux Balance Analysis (FBA), Flux Variability Analysis (FVA) or similar methods, can provide insights into the cellular metabolism, especially in combination with experimental data. Th...
Studying cell metabolism serves a plethora of objectives such as the enhancement of bioprocess performance, and advancement in the understanding of cell biology, of drug target discovery, and in metabolic therapy. Remarkable successes in these fields...
Maximizing the value of each available data point in bioprocess development is essential in order to reduce the time-to-market, lower the number of expensive wet-lab experiments, and maximize process understanding. Advanced in silico methods are incr...
A parallel-stirred tank bioreactor system on a 10 mL-scale automated with a liquid handling station introduces significant benefits in bioprocess analysis and design regarding preserving time, cost, and workload, thereby enabling quick generation of...
This work describes the evaluation of the solid-state fermentation (SSF) bioprocess utilizing brewery spent grain (BSG) and apple pomace (AP) as carbon sources and matrices for microorganism growth to produce xylanase, pectinase, and cellulase. The p...
SuperPro Designer® is a process simulator software used for analyzing the techno-economic feasibility of large-scale bioprocesses. Its predetermined built-in features allow for easy implementation by non-experts, but a lack of examples limits its...
Productivity improvements of mammalian cell culture in the production of recombinant proteins have been made by optimizing cell lines, media, and process operation. This led to enhanced titers and process robustness without increasing the cost of the...
Due to the increasing demand for greater safety and ease of scale bioprocessing, fault detection and diagnosis (FDD) is becoming an effective method to avoid breakdowns and disasters. Therefore, this work focuses on developing a dedicated observer-ba...
In recent years, advancements in microfluidic and sensor technologies have led to the development of new methods for monitoring cell growth both in macro- and micro-systems. In this paper, a microfluidic (MF) platform with a microbioreactor and integ...
Amoxicillin belongs to the β-lactam family of antibiotics, a class of highly consumed pharmaceutical products used for the treatment of respiratory and urinary tract infections, and is listed as a World Health Organisation (WHO) “Essential...
Uncertainty is a common feature of biological systems, and model-free extremum-seeking control has proved a relevant approach to avoid the typical problems related to model-based optimization, e.g., time- and resource-consuming derivation and identif...
Overflow metabolism is a phenomenon extended in nature, ranging from microbial to cancer cells. Accumulation of overflow metabolites pose a challenge for large-scale bioprocesses. Yet, the causes of overflow metabolism are not fully clarified. In thi...
Large-scale bioprocesses often suffer from spatial heterogeneities, which impact microbial performance and often lead to phenotypic population heterogeneity. To better understand these effects at the single-cell level, this study applied, for the fir...
Black soldier flies have been studied as an alternative animal feed. On the other hand, they could be used to yield an abundance of fatty acids. Their omnivorous diet and low space requirements allow for the mass breeding of black soldier flies, usin...
Efficient charge transport has been observed in iodine-doped, iodide-based room-temperature ionic liquids, yielding high ionic conductivity. To elucidate preferred mechanistic pathways for the iodide (
Anaerobic digestion processes offer the possibility for wastewater treatment while obtaining a benefit through the obtained biogas. This paper aims to continue the effort to adopt data-driven control methods in the case of anaerobic digestion process...
Glycoproteins represent the largest group of the growing number of biologically-derived medicines. The associated glycan structures and their distribution are known to have a large impact on pharmacokinetics. A modelling framework was developed to pr...
Implementing a personalised feeding strategy for each individual batch of a bioprocess could significantly reduce the unnecessary costs of overfeeding the cells. This paper uses lactate measurements during the cell culture process as an indication of...
The present work aimed to design a separation process for 2-phenylethanol (2-PEA) produced by whey fermentation and to evaluate its economic potential. The separation sequence consisted of a liquid–liquid extraction column followed by two disti...
In this research, a general numerical setting has been developed by finite volume approaching for the Eulerian–Eulerian method under OpenFOAM to provide an efficient reference for industrial bubbly flows having various geometrical characteristi...
Free-floating electrochemical sensors are promising for in situ bioprocess monitoring with the advantages of movability, a lowered risk of contamination, and a simplified structure of the bioreactor. Although floating sensors were developed for the m...
One of the key guidelines in the European waste management policy is the diversion of waste from landfills, preventing harmful effects on human health and the environment and ensuring that economically valuable waste materials are efficiently recycle...
With the development of agricultural biorefineries and bioprocessing operations, understanding the economic efficiencies and environmental impacts for these have gradually become popular for the deployment of these industrial processes. The corn-base...
Parenteral products appear to be sensitive to process conditions in bioprocessing steps, such as interfacial stress and shear stress. The combination of these elements is widely believed and proven to influence product stability, but the defined role...
Due to the ever-growing pressure on our planet’s natural resources to supply energy, the production of bioethanol by fermentation of lignocellulosic biomass is increasingly important in courses related to engineering and energy. Moreover, recen...
This review highlights the growing relevance of ion-exchange nanofibrous membranes (IEX-NFMs) in membrane chromatography (MC) for protein purification, emphasising their structural advantages such as high porosity, tunable surface functionality, and...
During the two years that have passed since the first volume of “Advances in Polyhydroxyalkanoate (PHA) production” was published, the progress in PHA-related research was indeed tremendous, calling for the next, highly bioprocess- and bi...
Industrial applications of xylanases in high-temperature settings are limited by enzyme instability. This study evaluated glycerol and phenolic compounds as modulators of the catalytic and structural properties of a recombinant Myceliophthora heterot...
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