Search Results (11)

Rice is a staple food for over half of the world’s population, and it is grown in over 100 countries. Rice blast disease can cause 10% to 30% crop loss, enough to feed 60 million people. Breeding for resistance can help farmers avoid costly fungicides. This study assessed the relationship between rice blast disease and zinc or anthocyanin content in biofortified rice. Susceptibility to foliar and panicle blast was assessed in a rice panel which differed on grain zinc content and pigmentation. A rice panel (n = 23) was challenged with inoculum of two isolates of Magnaporthe oryzae in a screenhouse-based assay. The zinc content with foliar blast severity was analyzed in the leaves and grain of a subset of non-inoculated rice plants. The effect of foliar zinc supplementation on seedlings was assessed by varying levels of zinc fertilizer solution on four blast susceptible cultivars at 14 days after planting (DAP), followed by inoculation with the blast pathogen at 21 DAP. Foliar blast severity was scored on a 0–9 scale at 7 days after inoculation. The rice panel was scored for anthocyanin content, and the data were correlated with foliar blast severity. The panel was grown in the field, and panicle blast, grain yield and yield-related agronomic traits were measured. Significant differences were observed in foliar blast severity among the rice genotypes, with IRBLK-KA and IR96248-16-2-3-3-B having mean scores greater than 4, as well as BASMATI 370 (a popular aromatic variety), while the rest of the genotypes were resistant. Supplementation with foliar zinc led to a significant decrease in susceptibility. A positive correlation was observed between foliar and panicle blast. The Zn in the leaves was negatively correlated with foliar blast severity, and had a marginally positive correlation with panicle blast. There was no relationship between foliar blast severity and anthocyanin content. Grain yield had a negative correlation with panicle blast, but no correlation was observed between Zn in the grain and grain yield. This study shows that Zn biofortification in the grain may not enhance resistance to foliar and panicle blast. Furthermore, the zinc-biofortified genotypes were not agronomically superior to the contemporary rice varieties. There is a need to apply genomic selection to combine promising alleles into adapted rice genetic backgrounds. Full article

Drought poses a significant threat to global food security, particularly impacting rice cultivation during the germination stage. In this study, a soil-based system that utilizes soil moisture content was used to simulate optimal and stress conditions to assess the effect of the specific seed priming protocols on germination. Eleven rice varieties, representative of indica and japonica subspecies, grown in different ecosystems and having diverse nutrient contents, were treated with water or solutions of either poly-gamma-glutamic acid (γ-PGA) or denatured γ-PGA. Collected data regarding germinability and stress indices revealed different drought sensitivity between japonica and indica subspecies and genotype-specific responses to priming. Particularly, γ-PGA improved germination of highly susceptible indica varieties whereas water soaking was more effective for the moderately sensitive japonica varieties. Integrative analyses evidenced differences between biofortified and non-biofortified rice under γ-PGA treatment, suggesting a possible correlation between γ-PGA efficacy and Zn/Fe seed content. These findings underline that priming strategies should be tailored based on genotype and therefore this factor should be always taken under consideration for future works. The current study provides relevant information for optimizing seed priming techniques to sustain the development of drought-resilient crops as a sustainable strategy to address agricultural resilience and safeguard food security amidst environmental challenges. Full article

Remote sensing data are powerful tools that contribute to sustainability and efficiency in crop management. Rice (Oryza sativa L.) is widely recognized as one of the most important crops in terms of economic and social impact. The aim of this study was to evaluate the efficiency of the use of Unmanned Aerial Vehicles (UAVs) in providing valuable information regarding plant health and status with respect to two rice varieties (Ariete and Ceres) submitted to a biofortification workflow with two types of selenium (sodium selenate and sodium selenite). In this context, through the use of synchronized UAVs, the state of the culture was further assessed. As well, digital elevation models, water lines, slope classes/infiltration suitability, and the Normalized Difference Vegetation Index (NDVI) were considered. Additionally, leaf gas exchange measurements were conducted during the biofortification process and Se content in rice was quantified. The NDVI index ranged from 0.76 to 0.80, with no significant differences regarding control. The water drainage pattern following the artificial pattern created by grooves between plots was observed. Furthermore, selenite application up to 100 g Se.ha⁻¹ did not exhibit toxicity effects on the biofortified plants and presented grain enrichment of 16.09 µg g⁻¹ (Ariete) and 15.46 µg g⁻¹ (Ceres). In conclusion, precision agriculture techniques and the utilization of data from leaf gas exchanges allow for efficient monitoring of experimental field conditions and are highly useful tools in decision-making. Full article

Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted using 85 diverse rice accessions from the Democratic Republic of Congo (DRC) to identify genomic regions associated with grain Fe and Zn content. The Fe content ranged from 0.95 to 8.68 mg/100 g on a dry weight basis (dwb) while Zn content ranged from 0.87 to 3.8 mg/100 g (dwb). Using MLM and FarmCPU models, we found 10 significant SNPs out of which one SNP on chromosome 11 was associated with the variation in Fe content and one SNP on chromosome 4 was associated with the Zn content, and both were commonly detected by the two models. Candidate genes belonging to transcription regulator activities, including the bZIP family genes and MYB family genes, as well as transporter activities involved in Fe and Zn homeostasis were identified in the vicinity of the SNP markers and selected. The identified SNP markers hold promise for marker-assisted selection in rice breeding programs aimed at enhancing Fe and Zn content in rice. This study provides valuable insights into the genetic factors controlling Fe and Zn uptake and their transport and accumulation in rice, offering opportunities for developing biofortified rice varieties to combat malnutrition among rice consumers. Full article

Globally, micronutrient (iron and zinc) enriched rice has been a sustainable and cost-effective solution to overcome malnutrition or hidden hunger. Understanding the genetic basis and identifying the genomic regions for grain zinc (Zn) across diverse genetic backgrounds is an important step to develop biofortified rice varieties. In this case, an RIL population (306 RILs) obtained from a cross between the high-yielding rice variety MTU1010 and the high-zinc rice variety Ranbir Basmati was utilized to pinpoint the genomic region(s) and QTL(s) responsible for grain zinc (Zn) content. A total of 2746 SNP markers spanning a genetic distance of 2445 cM were employed for quantitative trait loci (QTL) analysis, which resulted in the identification of 47 QTLs for mineral (Zn and Fe) and agronomic traits with 3.5–36.0% phenotypic variance explained (PVE) over the seasons. On Chr02, consistent QTLs for grain Zn polished (qZnPR.2.1) and Zn brown (qZnBR.2.2) were identified. On Chr09, two additional reliable QTLs for grain Zn brown (qZnBR.9.1 and qZnBR.9.2) were identified. The major-effect QTLs identified in this study were associated with few key genes related to Zn and Fe transporter activity. The genomic regions, candidate genes, and molecular markers associated with these major QTLs will be useful for genomic-assisted breeding for developing Zn-biofortified varieties. Full article

The demonstrations on improved rice production technology were organized in the Wanaparthy, Nagarkurnool, Yadadri Bhuvanagiri, and Rangareddy districts of Telangana state of India. These demonstrations were organized under the Scheduled Caste Sub Plan of the Indian Institute of Rice Research during the wet season of 2021. The biofortified varieties assume great significance to achieve nutrition security. Hence, the zinc biofortified rice variety, DRR Dhan 48 was demonstrated on the 142 farmer fields and the economic impact of these demonstrations was assessed with the ‘difference in difference’ approach. The results revealed that the production of rice under demonstration plots was more profitable with a higher benefit-to-cost ratio (B:C; 1.9) compared to that of the control plots (1.4). The independent two-sample t-test revealed that the productivity at the control plots was not statistically different from that of the demonstrations plots (p = 0.112) before the project was implemented, however, with the intervention in the form of the demonstration of improved rice production technology, the productivity differed significantly (p = 0.000) for the control and the demonstration plots for the intervention year. The results of the difference in differences estimator revealed that there was a positive impact of demonstrations on the yield of the beneficiaries. The mean productivity of demonstration plots and control plots were 5.52 t/ha and 4.5 t/ha, respectively. The farmers had an additional yield advantage of 22.6% over the control plots. The results indicated that the adoption of an improved package of practices would enable harnessing higher productivity levels and bridging the yield gaps in similar agroecosystems. Also, the results suggest the practical significance of the popularization of biofortified rice varieties for food and nutritional security. Full article

Remote sensed data already have an important role in crop management. In fact, NDVI (normalized difference vegetation index) has been use for staple crop management and monitorization since the 1980s, namely, in rice, wheat and maize. Accordingly, this study aimed to monitor, through precision agriculture, the development of a highly produced and consumed rice genotype in Portugal (Ariete variety), submitted to a selenium biofortification workflow. Rice biofortification was promoted during the production cycle, and assessed after two foliar applications with selenium (sprayed with 50 and 100 g Se·ha⁻¹ of sodium selenite). In this context, NDVI showed a high and identical value between control and biofortified plants, which indicated that the culture displayed a higher vigor and was in a healthy state of development despite foliar applications. Analyzes were further carried out for monitor the mobilization of photoassimilates, showing that plants did not demonstrate any negative impact on net photosynthesis and there was even a slight rise in the treatments. Additionally, to characterize the soil of the paddy rice field, some parameters were also analyzed, namely, organic matter, humidity, pH and electrical conductivity, being found that the parameters ranged between from 1.085–1.575%, 12.05–17.45%, 5.70–6.20, respectively, whereas the average conductivity was 223.4 µS cm⁻¹. Concerning to soil color, and considering the parameters L, a* and b* of the CIELab scale, significantly higher values in samples without humidity and without humidity and organic matter were found. In spite of the differences found, it is concluded that biofortification process did not affect any physiological parameters (net photosynthesis–Pn, stomatal conductance to water vapor—gs, transpiration rates—E and instantaneous water use efficiency—iWUE) in rice plants. Full article

Increasing demands for food and resources are challenging existing markets, driving a need to continually investigate and establish crop varieties with improved yields and health benefits. By the later part of the century, current estimates indicate that a >50% increase in the yield of most of the important food crops including wheat, rice and barley will be needed to maintain food supplies and improve nutritional quality to tackle what has become known as ‘hidden hunger’. Improving the nutritional quality of crops has become a target for providing the micronutrients required in remote communities where dietary variation is often limited. A number of methods to achieve this have been investigated over recent years, from improving photosynthesis through genetic engineering, to breeding new higher yielding varieties. Recent research has shown that growing plants under elevated [CO₂] can lead to an increase in Vitamin C due to changes in gene expression, demonstrating one potential route for plant biofortification. In this review, we discuss the current research being undertaken to improve photosynthesis and biofortify key crops to secure future food supplies and the potential links between improved photosynthesis and nutritional quality. Full article

One out of three humans suffer from micronutrient deficiencies called “hidden hunger”. Underprivileged people, including preschool children and women, suffer most from deficiency diseases and other health-related issues. Rice (Oryza sativa), a staple food, is their source of nutrients, contributing up to 70% of daily calories for more than half of the world’s population. Solving “hidden hunger” through rice biofortification would be a sustainable approach for those people who mainly consume rice and have limited access to diversified food. White milled rice grains lose essential nutrients through polishing. Therefore, seed-specific higher accumulation of essential nutrients is a necessity. Through the method of biofortification (via genetic engineering/molecular breeding), significant increases in iron and zinc with other essential minerals and provitamin-A (β-carotene) was achieved in rice grain. Many indica and japonica rice cultivars have been biofortified worldwide, being popularly known as ‘high iron rice’, ‘low phytate rice’, ‘high zinc rice’, and ‘high carotenoid rice’ (golden rice) varieties. Market availability of such varieties could reduce “hidden hunger”, and a large population of the world could be cured from iron deficiency anemia (IDA), zinc deficiency, and vitamin-A deficiency (VAD). In this review, different approaches of rice biofortification with their outcomes have been elaborated and discussed. Future strategies of nutrition improvement using genome editing (CRISPR/Cas9) and the need of policy support have been highlighted. Full article

This paper represents a series of in vitro iron (Fe) bioavailability experiments, Fe content analysis and polyphenolic profile of the first generation of Fe biofortified beans (Phaseolus vulgaris) selected for human trials in Rwanda and released to farmers of that region. The objective of the present study was to demonstrate how the Caco-2 cell bioassay for Fe bioavailability can be utilized to assess the nutritional quality of Fe in such varieties and how they may interact with diets and meal plans of experimental studies. Furthermore, experiments were also conducted to directly compare this in vitro approach with specific human absorption studies of these Fe biofortified beans. The results show that other foods consumed with beans, such as rice, can negatively affect Fe bioavailability whereas potato may enhance the Fe absorption when consumed with beans. The results also suggest that the extrinsic labelling approach to measuring human Fe absorption can be flawed and thus provide misleading information. Overall, the results provide evidence that the Caco-2 cell bioassay represents an effective approach to evaluate the nutritional quality of Fe-biofortified beans, both separate from and within a targeted diet or meal plan. Full article

Great progress has been made over the past decade with respect to the application of biotechnology to generate nutritionally improved food crops. Biofortified staple crops such as rice, maize and wheat harboring essential micronutrients to benefit the world’s poor are under development as well as new varieties of crops which have the ability to combat chronic disease. This review discusses the improvement of the nutritional status of crops to make a positive impact on global human health. Several examples of nutritionally enhanced crops which have been developed using biotechnological approaches will be discussed. These range from biofortified crops to crops with novel abilities to fight disease. The review concludes with a discussion of hurdles faced with respect to public perception, as well as directions of future research and development for nutritionally enhanced food crops. Full article