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13 pages, 2115 KB  
Article
Changed Trends in Utilization and Substitution Pattern of Non-National Immunization Program Vaccines in Central China, 2011–2024
by Lei Wang, Hao Li, Ling Zhang and Dan Li
Vaccines 2026, 14(1), 16; https://doi.org/10.3390/vaccines14010016 - 23 Dec 2025
Viewed by 191
Abstract
Objective: To explore the problems with non-National Immunization Program vaccinations in Hubei Province and to provide the basis for follow-up vaccination and management. Methods: Vaccination data on non-NIP/NIP vaccine doses were extracted from the Hubei Provincial Immunization Planning Information Management System. Descriptive epidemiological [...] Read more.
Objective: To explore the problems with non-National Immunization Program vaccinations in Hubei Province and to provide the basis for follow-up vaccination and management. Methods: Vaccination data on non-NIP/NIP vaccine doses were extracted from the Hubei Provincial Immunization Planning Information Management System. Descriptive epidemiological analyses were conducted to examine dose administration, vaccine-type composition, regional distribution, and substitution patterns. The trend χ2 test was used to assess temporal significance. Multistage regression analysis was performed using Joinpoint software. Results: From 2011 to 2024, a total of 91,009,259 doses (annual average: 6,500,661) with 35 types of non-NIP vaccines were administered in Hubei Province, China. The top five vaccines by doses administered were influenza vaccine, rabies vaccine, Hemophilus influenzae type b conjugate vaccine, varicella attenuated live vaccine, and enterovirus 71 inactivated vaccine. Before 2024 (2011–2023), vaccine utilization showed a long-term upward trend: per 10,000, population usage rose from 657.07 (2011) to a peak of 2393.21 (2023) (Increase: 264.22%, χ2 = 138.62, p < 0.05) (AAPC = 10.92%, p < 0.05) and non-NIP’s share of total vaccines increased from 25.52% (2011) to 65.95% (2023), (Increase: 154.33%, χ2 = 89.47, p < 0.05) (AAPC = 8.74%, p < 0.05). A notable reversal occurred in 2024. Non-NIP doses dropped from 13,971,544 (2023) to 10,238,861 (2024) with population usage falling from 2393.21 (2023) to 1755.03 (2024) (decrease: 26.66%) per 10,000, with the top three declines being in inactivated polio vaccine (IPV) (decrease: 49.53%), influenza vaccine (decrease: 44.21%), and oral rotavirus attenuated live vaccine (decrease: 43.50%). The total number of substitutive non-National Immunization Program (non-NIP) vaccine doses administered reached 16,618,755, with an overall substitution rate of 10.10%. This rate showed a steady upward trend from 5.57% in 2011 to 24.74% in 2023 (trend χ2 = 15.11, p < 0.05), yet it increased to 28.03% in 2024. Conclusions: Non-NIP vaccines and NIP-substitute use grew steadily for over a decade, then contracted sharply in 2024. Decision-makers should investigate the sudden dip, differentiate discretionary from replacement demand, and reallocate funds to sustain equity and prevent further erosion of coverage. Full article
(This article belongs to the Section Epidemiology and Vaccination)
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18 pages, 605 KB  
Article
Digestion in and Performance of Intensively Reared Beef Cattle Fed Diets with a Majority of Maize or Barley, Either Ground or Dry-Rolled
by Abdelmuhsen Al Alami, Antonio Gimeno, Sofía Schauf, Carlos Castrillo and Antonio de Vega
Ruminants 2026, 6(1), 1; https://doi.org/10.3390/ruminants6010001 - 22 Dec 2025
Viewed by 115
Abstract
Barley is more extensively and more rapidly fermentable than maize, thus it is supposed to increase digestive disorders in ruminants. However, the effect of cereal type on animal performance and digestion may vary with processing degree. In the present experiment, the effect of [...] Read more.
Barley is more extensively and more rapidly fermentable than maize, thus it is supposed to increase digestive disorders in ruminants. However, the effect of cereal type on animal performance and digestion may vary with processing degree. In the present experiment, the effect of dry-rolling or grinding barley and maize, as the main cereals in a concentrate containing a high proportion of starch with different rates of fermentation, on intensively reared beef cattle performance, diet digestibility, and feed intake amount and pattern, was studied. Thirty-six 3-month-old male calves were allocated to one of four diets consisting of barley straw (BS) and a concentrate with 60% cereals (barley and maize in proportions 75:25 or 25:75) presented dry-rolled or ground through a 3.5 mm sieve. The experimental period was divided into two phases of 10 weeks each: from start to 277 ± 3.6 kg live weight (LW; Growing), and from 289 ± 3.8 kg LW to slaughter (399 ± 4.6 kg; Finishing). For the Growing phase, there were no differences (p > 0.10) between the majority cereal in the concentrates, nor between their processing methods, in the daily intake of concentrate and BS, and in the animals’ final LW. With respect to Finishing, the interaction between cereal type and processing was significant (p < 0.05) for concentrate daily intake. As a result, animals consuming ground barley ate less concentrate than those fed rolled barley, whereas there were no differences between processing methods for animals fed maize-based diets. Animals consuming ground-barley concentrates consumed significantly more straw than those fed on dry-rolled-barley concentrates (p < 0.05 for Growing and p < 0.01 for Finishing) during the first four hours after feeding. No such differences appeared in animals consuming maize-based concentrates. Starch digestibility was higher in animals fed ground cereals vs. dry-rolled cereals during the Growing phase (p = 0.048), whereas NDF digestibility was also higher (p = 0.008) in animals fed ground cereals during the Finishing phase. The faeces from animals fed on rolled-maize concentrates showed a higher concentration of purine bases than the faeces of animals fed on rolled-barley concentrates (p = 0.016), although there were no differences for the ground cereals. Overall, the results reported indicated that replacing maize with barley in diets for feedlot beef cattle did not affect average daily gain, intake of straw or concentrate, or feed conversion ratios (total or considering just the concentrate); hence the inclusion of either cereal in greater proportions should be based on their market price and on the final cost of the compound feed (which may include different ingredients). The processing method of the cereals (grinding or dry-rolling) also had no influence on the above-mentioned variables, so the selection of the method should be based on their relative cost, exclusively in terms of feed efficiency. Full article
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28 pages, 4040 KB  
Article
Research on Rail Damage Detection Based on Improved DETR Algorithm
by Sanxiu Wu, Mengquan Wu, Fengtao Lin, Yang Yang and Rongkai Tan
Appl. Sci. 2025, 15(24), 13223; https://doi.org/10.3390/app152413223 - 17 Dec 2025
Viewed by 150
Abstract
In rail damage detection, the scale variation of small targets leads to inaccurate extraction of damage morphology and size features, thereby affecting the reliable identification of damage types. The DETR algorithm has been optimized and improved. Firstly, we introduce the convolution–attention fusion module [...] Read more.
In rail damage detection, the scale variation of small targets leads to inaccurate extraction of damage morphology and size features, thereby affecting the reliable identification of damage types. The DETR algorithm has been optimized and improved. Firstly, we introduce the convolution–attention fusion module (CAFMAttention) after the two side convolutional layers of the original algorithm; then, we replace the nn.Upsample-based upsampling layer with the Dysample upsampler. Finally, we replace the Conv modules in the two down-sampled convolutional layers with Dual-Conv modules. The results of the comparative experiments show that the recall rate of the improved DETR model in this paper is 0.698, which is 12.2% higher than that of the original DETR model. The accuracy is 0.815, which is 2.3% higher than that of the original DETR model. The average precision (Map@0.5) is 0.741. Compared with the original DETR model, it has been improved by 8.7%. The F1 score is 0.75, which is 8.7% higher than the original DETR model. The frame per second (FPS) transfer rate is 64.94, which is 2.6% higher than that of the original DETR model. The proposed DETR algorithm can detect rail damage under complex working conditions well, with high accuracy and robustness, and better meet the requirements of practical actual rail detection. Full article
(This article belongs to the Section Transportation and Future Mobility)
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24 pages, 2467 KB  
Article
Assessment of Decarbonization Scenarios for the Portuguese Road Sector
by João Salvador, Gonçalo O. Duarte and Patrícia C. Baptista
Energies 2025, 18(24), 6587; https://doi.org/10.3390/en18246587 - 17 Dec 2025
Viewed by 125
Abstract
This study presents a scenario-based modeling framework to evaluate potential decarbonization pathways for Portugal’s road transport sector. The model simulates the evolution of a light-duty vehicle (LDV) fleet under varying degrees of electrification and biofuel integration, accounting for energy consumption, CO2 emissions [...] Read more.
This study presents a scenario-based modeling framework to evaluate potential decarbonization pathways for Portugal’s road transport sector. The model simulates the evolution of a light-duty vehicle (LDV) fleet under varying degrees of electrification and biofuel integration, accounting for energy consumption, CO2 emissions and market shares of alternative propulsion technologies. Coupled with projected energy mix trajectories, the framework estimates final energy demand and well-to-wheel (WTW) emissions for each scenario, benchmarking outcomes against national and European climate targets. A key structural limitation identified is the long vehicle survival rate—averaging 14 years—which constrains fleet renewal and delays the transition to full electrification. Diesel-powered light commercial vehicles exhibit even slower replacement dynamics, rendering the Portuguese targets of full electrification by 2030 highly improbable without targeted scrappage and incentive programs. Scenario analysis indicates that even with accelerated electric vehicle (EV) uptake, battery electric vehicles (BEVs) would comprise only 12% of the fleet by 2030 and 77% by 2050. Electrification scenario raises electricity demand fortyfold by 2050, stressing generation and infrastructure. Scenarios that consider diversification of energy sources reduce this strain but require triple electricity for large-scale green hydrogen and synthetic fuel production. Full article
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14 pages, 2582 KB  
Article
Seafood Object Detection Method Based on Improved YOLOv5s
by Nan Zhu, Zhaohua Liu, Zhongxun Wang and Zheng Xie
Sensors 2025, 25(24), 7546; https://doi.org/10.3390/s25247546 - 12 Dec 2025
Viewed by 293
Abstract
To address the issues of false positives and missed detections commonly observed in traditional underwater seafood object detection algorithms, this paper proposes an improved detection method based on YOLOv5s. Specifically, we introduce a Spatial–Channel Synergistic Attention (SCSA) module after the Fast Spatial Pyramid [...] Read more.
To address the issues of false positives and missed detections commonly observed in traditional underwater seafood object detection algorithms, this paper proposes an improved detection method based on YOLOv5s. Specifically, we introduce a Spatial–Channel Synergistic Attention (SCSA) module after the Fast Spatial Pyramid Pooling layer in the backbone network. This module adopts a synergistic mechanism where the channel attention guides spatial localization, and the spatial attention feeds back to optimize channel weights, dynamically enhancing the unique features of aquatic targets (such as sea cucumber folds) while suppressing seawater background interference. In addition, we replace some C3 modules in YOLOv5s with our designed three-scale convolution dual-path variable-kernel module based on Pinwheel-shaped Convolution (C3k2-PSConv). This module strengthens the model’s ability to capture multi-dimensional features of aquatic targets, especially in the feature extraction of small-sized and occluded targets, reducing the false detection rate while ensuring the model’s lightweight property. The enhanced model is evaluated on the URPC dataset, which contains real-world underwater imagery of echinus, starfish, holothurian, and scallop. The experimental results show that compared with the baseline model YOLOv5s, while maintaining real-time inference speed, the proposed method in this paper increases the mean average precision (mAP) by 2.3% and reduces the number of parameters by approximately 2.4%, significantly improving the model’s operational efficiency. Full article
(This article belongs to the Section Sensing and Imaging)
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13 pages, 681 KB  
Systematic Review
Hydroponic Forage in Ruminant Nutrition: A Systematic Review of Nutritional Value, Performance Outcomes, and Sustainability
by Alessandro Vastolo and Monica Isabella Cutrignelli
Animals 2025, 15(24), 3544; https://doi.org/10.3390/ani15243544 - 9 Dec 2025
Viewed by 489
Abstract
This systematic review evaluated 28 peer-reviewed studies on the use of hydroponic forage in ruminant diets, following PRISMA 2020 guidelines. Hydroponic barley sprouts contain on average 14.8 ± 2.1% CP, 3.6 ± 0.4% EE, 12.9 ± 1.7% NDF, 7.8 ± 1.2% ADF, and [...] Read more.
This systematic review evaluated 28 peer-reviewed studies on the use of hydroponic forage in ruminant diets, following PRISMA 2020 guidelines. Hydroponic barley sprouts contain on average 14.8 ± 2.1% CP, 3.6 ± 0.4% EE, 12.9 ± 1.7% NDF, 7.8 ± 1.2% ADF, and 10.5 ± 2.8% DM (mean ± SD; n = 21 studies), and mineral content, though limited by high moisture and low dry matter yield. Among the included studies, 61% focused on barley, confirming its suitability for hydroponic cultivation. In dairy cattle, hydroponic forage improved milk fat content and oleic acid (C18:1), linoleic acid (C18:2), and α-linolenic acid (C18:3) and reduced saturated fatty acids without compromising yield. In buffaloes, inclusion enhanced cheese quality and reduced energy footprint, though costs were higher. For small ruminants and growing animals, moderate inclusion (5–25% dry matter) improved intake, digestibility, and growth, while excessive replacement reduced feed intake or digestibility, likely due to rumen microbiota shifts. Additionally, hydroponic feeding reduced methane emissions in lambs, highlighting its environmental potential. Overall, hydroponic forage can serve as a sustainable complement to conventional feed resources, promoting resource efficiency and animal performance when properly integrated into balanced diets. Further studies should define optimal inclusion rates and evaluate economic and environmental trade-offs under different production systems. Full article
(This article belongs to the Section Animal Nutrition)
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18 pages, 1560 KB  
Article
Transmission Line Bird Species Detection and Identification Based on Double Data Enhancement and Improvement of YOLOv8s
by Tao Xue, Dingyue Cheng, Tao Chen, Rui Zhao, Zhenhao Wang and Chong Wang
Appl. Sci. 2025, 15(24), 12953; https://doi.org/10.3390/app152412953 - 9 Dec 2025
Viewed by 167
Abstract
To address the challenge of bird species detection on transmission lines, this paper proposes a detection method based on dual data enhancement and an improved YOLOv8s model. The method aims to improve the accuracy of identifying small- and medium-sized targets in bird detection [...] Read more.
To address the challenge of bird species detection on transmission lines, this paper proposes a detection method based on dual data enhancement and an improved YOLOv8s model. The method aims to improve the accuracy of identifying small- and medium-sized targets in bird detection scenes on transmission lines, while also accounting for the impact of changing weather conditions. To address these issues, a dual data enhancement strategy is introduced. The model’s generalization ability in outdoor environments is enhanced by simulating various weather conditions, including sunny, cloudy, and foggy days, as well as halo effects. Additionally, an improved Mosaic augmentation technique is proposed, which incorporates target density calculation and adaptive scale stitching. Within the improved YOLOv8s architecture, the CBAM attention mechanism is embedded in the Backbone network, and BiFPN replaces the original Neck module to facilitate bidirectional feature extraction and fusion. Experimental results demonstrate that the proposed method achieves high detection accuracy for all bird species, with an average precision rate of 94.2%, a recall rate of 89.7%, and an mAP@50 of 94.2%. The model also maintains high inference speed, demonstrating potential for real-time detection requirements. Ablation and comparative experiments validate the effectiveness of the proposed model, confirming its suitability for edge deployment and its potential as an effective solution for bird species detection and identification on transmission lines. Full article
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18 pages, 2965 KB  
Article
Optimizing the Transformer Iron Core Cutting Stock Problem Using a Discrete Artificial Bee Colony Algorithm
by Qiang Luo, Zuogan Tang and Chunrong Pan
Machines 2025, 13(12), 1106; https://doi.org/10.3390/machines13121106 - 28 Nov 2025
Viewed by 311
Abstract
In the manufacturing of iron core for high-power transformers, a cutting stock problem arises where large-width silicon steel coils must be cut into narrower coils, known as strips. Typically, the required length of each strip far exceeds that of a single coil. Therefore, [...] Read more.
In the manufacturing of iron core for high-power transformers, a cutting stock problem arises where large-width silicon steel coils must be cut into narrower coils, known as strips. Typically, the required length of each strip far exceeds that of a single coil. Therefore, the problem necessitates additional consideration of how to split the strips and arrange them on the large coils, with the goal of minimizing the total number of strips. In this paper, we propose a discrete artificial bee colony algorithm to address this problem. The algorithm replaces the stochastic roulette wheel with biased selection in the onlooker bee phase and introduces partially mapped crossover in both the onlooker and scout bee phases. These enhancements facilitate more effective utilization of information from high-quality solutions, thereby improving the algorithm’s stability and its capacity to obtain higher-quality results. Experimental results show that compared to existing methods reported in the literature, the proposed approach reduces the total number of strips by an average of over 3.9% and 7.6% for Set 2 and Set 3, respectively, while also exhibiting a faster convergence rate than other competitive algorithms. Full article
(This article belongs to the Section Advanced Manufacturing)
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11 pages, 1626 KB  
Article
A Question of Dose? Ultra-Low Dose Chest CT on Photon-Counting CT in People with Cystic Fibrosis
by Marcel Opitz, Matthias Welsner, Halil I. Tazeoglu, Florian Stehling, Sivagurunathan Sutharsan, Dirk Westhölter, Erik Büscher, Christian Taube, Nika Guberina, Denise Bos, Marcel Drews, Daniel Rosok, Sebastian Zensen, Johannes Haubold, Lale Umutlu, Michael Forsting and Marko Frings
Tomography 2025, 11(12), 134; https://doi.org/10.3390/tomography11120134 - 27 Nov 2025
Viewed by 442
Abstract
Objective: Chest computed tomography (CT) is a key component of the diagnostic assessment of people with cystic fibrosis (PwCF) and is increasingly replacing chest radiography. Due to improvements in life expectancy, radiation exposure has become a growing concern in PwCF. Photon-counting CT (PCCT) [...] Read more.
Objective: Chest computed tomography (CT) is a key component of the diagnostic assessment of people with cystic fibrosis (PwCF) and is increasingly replacing chest radiography. Due to improvements in life expectancy, radiation exposure has become a growing concern in PwCF. Photon-counting CT (PCCT) has the potential to reduce the risk of radiation-induced malignancies while maintaining diagnostic accuracy. This study aimed to compare the radiation dose and image quality of low-dose high-resolution (LD-HR) and ultra-low-dose high-resolution (ULD-HR) CT protocols using PCCT in PwCF. Methods: This retrospective study included 72 PwCF, with 36 undergoing a LD-HR chest CT protocol and 36 receiving an ULD-HR protocol on a PCCT. The radiation dose and image quality were assessed by comparing the effective dose and signal-to-noise ratio (SNR). Three blinded radiologists evaluated the overall image quality, sharpness, noise, and assessability of the bronchi, bronchial wall thickening, and bronchiolitis using a five-point Likert scale. Results: The ULD-HR PCCT protocol reduced radiation exposure by approximately 65% compared with the LD-HR PCCT protocol (median effective dose: 0.19 vs. 0.55 mSv, p < 0.001). While LD-HR images were consistently rated higher than ULD-HR images (p < 0.001), both protocols maintained diagnostic significance (median image quality rating of “4-good”). The average SNR of the lung parenchyma was significantly lower with ULD-HR PCCT compared to LD-HR PCCT (p < 0.001). Conclusions: ULD-HR PCCT significantly reduced radiation exposure while maintaining good diagnostic image quality in PwCF. The effective dose of ULD-HR PCCT is only twice that of a two-plane chest X-ray, making it a viable low-radiation alternative for routine imaging in PwCF. Full article
(This article belongs to the Special Issue Medical Image Analysis in CT Imaging)
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12 pages, 1380 KB  
Article
Unsupervised Clustering of 41,728 Emergency Department Visits: Insights into Patient Profiles and KTAS Reliability
by Jongsun Kim, EunChul Jang, SoonChan Kwon and MyoungJe Song
Healthcare 2025, 13(23), 3073; https://doi.org/10.3390/healthcare13233073 - 26 Nov 2025
Viewed by 318
Abstract
Introduction: In the emergency room, it is essential to quickly and accurately classify the patients’ various severities. However, existing five-stage classification systems, such as the Korean Emergency Patient Classification Tool (KTAS), do not sufficiently reflect the physiological and clinical heterogeneity of all patients, [...] Read more.
Introduction: In the emergency room, it is essential to quickly and accurately classify the patients’ various severities. However, existing five-stage classification systems, such as the Korean Emergency Patient Classification Tool (KTAS), do not sufficiently reflect the physiological and clinical heterogeneity of all patients, so there is a possibility of under-classification in some age groups or specific symptom groups. Methods: A retrospective cross-sectional study was conducted using KTAS and the physiological and clinical data of 41,728 patients who visited the emergency room of a university hospital in Incheon in 2022. K-prototypes unsupervised cluster analysis incorporating demographic, physiological, and clinical variables was applied, and the number of clusters was determined as the optimal value through the Silhouette, Dunn, and Davies–Bouldin indicators. Dimension reduction was performed by UMAP, and differences between clusters were compared by t-test, Mann–Whitney U, and chi-square test. Results: Two different clusters were identified. Cluster 0 was a stable patient group with a mean age of 58 years and an average arterial pressure of 104 mmHg. On the other hand, Cluster 1 was a young but physiologically unstable patient group with an average age of 46 years and an average arterial pressure of 90 mmHg. There were significant differences in age, MAP, heart rate, respiratory rate, body temperature, and pain scores between clusters (p < 0.001), and a moderate association was observed between KTAS classification and clusters (Cramer’s V = 0.208). Discussion: This study suggested the possibility of early identification of high-risk groups in the emergency room and efficient resource allocation by identifying potential patient heterogeneity that KTAS cannot detect through unsupervised learning. This approach can be used as a basis for precision triage and patient-centered emergency medical policy establishment by supplementing rather than replacing the existing classification system. Full article
(This article belongs to the Special Issue New Tools and Technologies in Emergency Medicine and Critical Care)
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18 pages, 2805 KB  
Article
An Improved YOLOv11 Recognition Algorithm for Heavy-Duty Trucks on Highways
by Junkai Guo and Mingjiang Zhang
Electronics 2025, 14(23), 4621; https://doi.org/10.3390/electronics14234621 - 25 Nov 2025
Viewed by 295
Abstract
This paper presents an enhanced YOLOv11-based algorithm for highway freight truck tarpaulin recognition to enhance real-time performance and accuracy in identifying truck axle types and tarpaulin materials. The proposed methodology incorporates four key innovations. First, the lightweight Spatial and Channel Reconstruction Convolution (SCConv) [...] Read more.
This paper presents an enhanced YOLOv11-based algorithm for highway freight truck tarpaulin recognition to enhance real-time performance and accuracy in identifying truck axle types and tarpaulin materials. The proposed methodology incorporates four key innovations. First, the lightweight Spatial and Channel Reconstruction Convolution (SCConv) module is introduced to replace standard convolutional layers in the YOLOv11 backbone feature extraction network, which enables maintaining strong feature extraction capabilities while reducing model parameters and computational complexity. Second, a Channel-Spatial Multi-scale Attention Module (CSMAM) is integrated with the C3k2 module of the YOLOv11 feature fusion network, thereby strengthening the network’s capacity to learn both truck body features and tarpaulin coverage characteristics. Third, a novel Dual-Enhanced Channel Detection Head (DEC-Head) detector is designed to improve recognition performance under ambiguous conditions and reduce parameter quality. Finally, the SIoU loss function is adopted to replace the conventional bounding box loss function, substantially improving prediction box accuracy. Comprehensive experimental results demonstrate that compared to the baseline YOLOv11 algorithm, our proposed method achieves an approximate 4.4% increase in precision, 5.2% improvement in recall rate, and 7.2% higher mean Average Precision (mAP), while also achieving a significant improvement in inference speed (Frames Per Second, FPS), establishing superior recognition performance for truck tarpaulin detection tasks. Full article
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14 pages, 1761 KB  
Article
In Vitro Propagation Protocol for Porlieria chilensis: Efficient Ex Vitro Rooting and Acclimatization
by Francesca Guerra, Loreto Badilla, Ricardo Cautín and Mónica Castro
Horticulturae 2025, 11(11), 1410; https://doi.org/10.3390/horticulturae11111410 - 20 Nov 2025
Cited by 1 | Viewed by 670
Abstract
Porlieria chilensis Johnst. (guayacán), an endemic Chilean species native to the sclerophyllous forests, is experiencing a significant population decline. Typically growing as a shrub or small tree on sunny slopes and rocky soils, its populations have been severely impacted by overexploitation and habitat [...] Read more.
Porlieria chilensis Johnst. (guayacán), an endemic Chilean species native to the sclerophyllous forests, is experiencing a significant population decline. Typically growing as a shrub or small tree on sunny slopes and rocky soils, its populations have been severely impacted by overexploitation and habitat degradation, leading to its classification as Vulnerable (VU). Mature, well-developed individuals have become increasingly rare, now mostly replaced by shrubby regrowth. This study presents the first reported in vitro propagation method for P. chilensis, aimed at establishing a protocol for its mass propagation. A 92.0% survival rate was achieved for nodal segments cultured on Murashige and Skoog (MS) medium after surface disinfection with 1.5% sodium hypochlorite for 15 min. Shoot elongation was successfully stimulated by supplementing the medium with 8.88 μM of 6-benzylaminopurine (BAP), resulting in an average shoot length of 6.9 cm. For ex vitro rooting, plants were transferred to 200 mL containers filled with a 2:1 (v/v) peat/perlite substrate. High rooting rates were obtained (87.5% and 93.1%) when treated with 1968 μM and 1476 μM of indole-3-butyric acid (IBA), respectively. Moreover, 89.8% of the plants survived acclimatization under controlled conditions. These results highlight the strong potential of in vitro propagation as an effective strategy for the conservation and restoration of P. chilensis populations. Full article
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18 pages, 2000 KB  
Article
Perioperative Patient Blood Management in Primary Knee and Hip Arthroplasty—Nonsense or Necessity?
by Johannes Neugebauer, Clemens Strassegger, David Putzer, Melanie Schindler, Adriana Palacio-Giraldo, Markus Neubauer, Gianpaolo Leone, Herbert Koinig and Dietmar Dammerer
J. Clin. Med. 2025, 14(22), 8237; https://doi.org/10.3390/jcm14228237 - 20 Nov 2025
Viewed by 449
Abstract
Background/Objectives: The perioperative management of a patient’s blood values involves the optimization of anemia management in the pre-, intra-, and postoperative periods to avoid blood transfusions. The purpose of this study was to determine the prevalence of pre- and postoperative anemia in patients [...] Read more.
Background/Objectives: The perioperative management of a patient’s blood values involves the optimization of anemia management in the pre-, intra-, and postoperative periods to avoid blood transfusions. The purpose of this study was to determine the prevalence of pre- and postoperative anemia in patients undergoing elective knee and hip arthroplasty, and explore the change in hemoglobin levels in transfused and non-transfused patients over the course of their hospital stay. Methods: In this retrospective study, data on anemia-influencing factors were collected and examined using multiple regression analysis (OLS model) to determine if they are associated with the average hemoglobin change in 801 patients who underwent a primary knee or hip arthroplasty. The study group was screened for the prevalence of preoperative anemia, and the incidence of postoperative anemia was examined. Results: A total of 801 patients were retrospectively investigated. The length of stay for the two different procedures was significantly different (p < 0.001). There was a markedly higher transfusion rate in the cases requiring longer-than-usual hospital stays. In total, 37 patients (5%) received perioperative erythrocyte transfusions. Total hip replacement (THR) was associated with significantly more erythrocyte transfusions (8%) in comparison with total knee replacement (TKR) (2%) (p < 0.001). Conclusions: Satisfactory results for the prevalence and incidence of anemia in the context of primary knee or hip arthroplasties were obtained in this study. The collected values indicate the need for better implementation and awareness of the importance of adequate patient blood management. Full article
(This article belongs to the Section Orthopedics)
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21 pages, 2863 KB  
Article
Evaluation of Functional Marine Protein Hydrolysates as Fish Meal Replacements in Low-Fish-Meal Diets: Effects on Growth Performance, Feed Utilization, and Health Status of Asian Seabass (Lates calcarifer)
by Dachawat Poonnual, Siriporn Tola and Bundit Yuangsoi
Animals 2025, 15(22), 3285; https://doi.org/10.3390/ani15223285 - 13 Nov 2025
Viewed by 714
Abstract
An eight-week study was conducted to evaluate the effects of dietary marine protein hydrolysates as fish meal replacements in low-fish-meal diets on the growth performance, feed utilization, and health status of Asian seabass (Lates calcarifer). The high-fish-meal (HFM) diet contained 25% [...] Read more.
An eight-week study was conducted to evaluate the effects of dietary marine protein hydrolysates as fish meal replacements in low-fish-meal diets on the growth performance, feed utilization, and health status of Asian seabass (Lates calcarifer). The high-fish-meal (HFM) diet contained 25% fish meal, while the low-fish-meal (LFM) diet replaced 60% of the fish meal with soybean meal. Three experimental diets were formulated by supplementing the LFM diet with 5% tuna hydrolysate (TH), 2% shrimp hydrolysate (SH), and 5% salmon silage (SS), each replacing an equivalent amount of fish meal. These diets were designated as LFM + TH, LFM + SH, and LFM + SS, respectively. The results showed that the LFM + TH diet significantly improved the percentage of weight gain, average daily growth, specific growth rate, protein efficiency ratio, and feed conversion ratio compared to the LFM diet (p < 0.05), without negatively affecting feed intake or metabolic markers. Histological analysis revealed improved villus length and goblet cell count in the intestine, indicating better nutrient absorption (p < 0.05). However, no significant differences were observed in hematological and immunological parameters, blood plasma metabolic markers, or carcass proximate composition (p > 0.05). Furthermore, the LFM + TH diet exhibited superior survival rates under ammonia stress, highlighting its potential to enhance stress tolerance. These findings suggest that marine protein hydrolysates, particularly 5%TH, can serve as a sustainable and efficient alternative to fish meal protein in diets with up to 60% in soybean meal compensation, promoting better growth and survival in Asian seabass. Full article
(This article belongs to the Section Aquatic Animals)
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14 pages, 2355 KB  
Article
Evaluation of Extreme Sea Level Flooding Risk to Buildings in Samoa
by Ryan Paulik, Shaun Williams, Josephina Chan-Ting, Cyprien Bosserelle, Antonio Espejo, Moritz Wandres, Katie Pogi, Sujina Vaimagalo, Rose Pearson, Judith Giblin, Luisa Hosse, James Battersby, Juliana Ungaro, Herve Damlamian and Orisi Naivalurua
J. Mar. Sci. Eng. 2025, 13(11), 2143; https://doi.org/10.3390/jmse13112143 - 12 Nov 2025
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Abstract
This study presents an economic risk evaluation of buildings in Samoa exposed to extreme sea level (ESL)-driven episodic flooding and permanent inundation from relative sea level (RSL) rise. A spatiotemporal risk analysis framework was applied at the building object level to calculate monetary [...] Read more.
This study presents an economic risk evaluation of buildings in Samoa exposed to extreme sea level (ESL)-driven episodic flooding and permanent inundation from relative sea level (RSL) rise. A spatiotemporal risk analysis framework was applied at the building object level to calculate monetary loss, expressed as the exceedance probability loss (EPL) and average annual loss (AAL). Economic risk was enumerated at national and district levels between the period 2020 and 2140 based on RSL projections for medium confidence Shared Socioeconomic Pathways (SSPs). Over this century, national AAL for buildings from ESL flooding in 2020 is expected to double by 2100 (USD 47–51 million). Under high emissions scenarios SSP3-7.0 and SSP5-8.5, AAL rates decelerate after 2100 as permanent inundation loss increases. District level risk variability is evident. For example, Tuamasaga on Upolu Island accounted for 44% of national 100-year annual recurrence interval losses, while AAL for Aiga-i-le-Tai and Va’a-o-Fonoti over this century reaches 8% of total district building replacement values. Our model approach has potential future applications to evaluate spatiotemporal risk distribution for a broader range of socioeconomic impacts that may occur beyond directly affected flood inundation areas. Full article
(This article belongs to the Section Coastal Engineering)
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