Search Results (5,845)

The inherent complexity and heterogeneity of tumors pose substantial challenges for the development of effective oncology therapeutics. Organoids, three-dimensional (3D) in vitro models, have become essential tools for predicting therapeutic responses and advancing precision oncology, with established correlations to clinical outcomes in patient-derived models. These systems have transformed preclinical drug screening by bridging the gap between conventional two-dimensional (2D) cultures and in vivo models, preserving tumor histopathology, cellular heterogeneity, and patient-specific molecular profiles. Despite their potential, limitations in tumor organoid biology, including inter-batch variability and microenvironmental simplification, can undermine their reliability and scalability in large-scale drug screening. To overcome these challenges, the integration of advanced technologies such as artificial intelligence (AI), automated biomanufacturing, multi-omics analytics, and vascularization strategies has been explored. This review highlights the “Organoid plus and minus” framework, which combines technological augmentation with culture system refinement to improve screening accuracy, throughput, and physiological relevance. We are convinced that the future of drug development hinges on the convergence of these multidisciplinary technologies with standardized biobanking and co-clinical validation frameworks. This integration will position organoids as a cornerstone for personalized drug discovery and therapeutic optimization, ultimately advancing the development of efficacy in oncology. Full article

The convergence of Industrial Internet of Things (IIoT) and digital twin technology offers new paradigms for process automation and control. This paper presents an integrated IIoT and digital twin framework for intelligent control of a gas–liquid separation unit with interacting flow, pressure, and differential pressure loops. A comprehensive dynamic model of the three-loop separator process is developed, linearized, and validated. Classical stability analyses using the Routh–Hurwitz criterion and Nyquist plots are employed to ensure stability of the control system. Decentralized multi-loop proportional–integral–derivative (PID) controllers are designed and optimized using the Integral Absolute Error (IAE) performance index. A digital twin of the separator is implemented to run in parallel with the physical process, synchronized via a Kalman filter to real-time sensor data for state estimation and anomaly detection. The digital twin also incorporates structured singular value ($\mu$) analysis to assess robust stability under model uncertainties. The system architecture is realized with low-cost hardware (Arduino Mega 2560, MicroMotion Coriolis flowmeter, pneumatic control valves, DAC104S085 digital-to-analog converter, and ENC28J60 Ethernet module) and software tools (Proteus VSM 8.4 for simulation, VB.Net 2022 version based human–machine interface, and ML.Net 2022 version for predictive analytics). Experimental results demonstrate improved control performance with reduced overshoot and faster settling times, confirming the effectiveness of the IIoT–digital twin integration in handling loop interactions and disturbances. The discussion includes a comparative analysis with conventional control and outlines how advanced strategies such as model predictive control (MPC) can further augment the proposed approach. This work provides a practical pathway for applying IIoT and digital twins to industrial process control, with implications for enhanced autonomy, reliability, and efficiency in oil and gas operations. Full article

Industrial microorganisms have a wide range of applications in biomanufacturing, energy production, environmental protectionpharmaceutical development, etc. Synthetic biology has revolutionised the field of industrial microorganisms by designing, constructing and optimising biological systems. The aim of this study is to discuss the key technologies of synthetic biology in industrial microorganisms and their application prospects. Gene editing technology, one of the core tools of synthetic biology, enables researchers to precisely modify microbial genomes to optimise their metabolic pathways or introduce new functions. Metabolic engineering, as an important direction for the application of synthetic biology in industrial microorganisms, enables the efficient synthesis of target products by optimising and reconstructing the metabolic pathways of microorganisms. The development of high-throughput screening and automated platforms has enabled large-scale gene editing and metabolic engineering experiments. The application of synthetic genomics promises to develop microbes with highly customised functions. However, there are still many challenges in this field, and future research still requires interdisciplinary collaboration to drive the application of synthetic biology in industrial microorganisms to new heights. Full article

State Departments of Transportation (DOTs) face challenges with traditional bridge inspections that are time-consuming, inconsistent, and paper-based. This study focused on an existing research gap regarding automated methods that streamline the bridge inspection process, prioritize maintenance effectively, and allocate resources efficiently. Thus, this paper introduces a digitalized bridge inspection framework by integrating Building Information Modeling (BIM) and Business Intelligence (BI) to enable near-real-time monitoring and digital documentation. This study adopts a Design Science Research (DSR) methodology, a recognized paradigm for developing and evaluating the innovative SmartBridge to address pressing bridge inspection problems. The method involved designing an Autodesk Revit-based plugin for data synchronization, element-specific comments, and interactive dashboards, demonstrated through an illustrative 3D bridge model. An illustrative example of the digitalized bridge inspection with the proposed framework is provided. The results show that SmartBridge streamlines data collection, reduces manual documentation, and enhances decision-making compared to conventional methods. This paper contributes to this body of knowledge by combining BIM and BI for digital visualization and predictive analytics in bridge inspections. The proposed framework has high potential for hybridizing digital technologies into bridge infrastructure engineering and management to assist transportation agencies in establishing a safer and efficient bridge inspection approach. Full article

Background: Recent advances in antibody discovery technologies, especially progress in de novo synthesis through machine learning, have imposed a significant production challenge for the generation of a large diversity of antibodies against nearly any target of interest. There is a demand for the rapid production of dozens of purified antibodies in 10-milligram quantities sufficient for functional screening and molecular assessment studies. Objectives: To meet this requirement, a semi-automated production methodology and workflow was developed to bridge the miniaturized high-throughput screenings (HTSs) and the conventional custom-scale workflow by taking advantage of four new technology applications. Methods: First, it exploited a novel, simple, high-titer transient expression system, “CHO4Tx^®”, which could achieve high yields in the range of 200 mg/L and above, across a variety of antibody constructs, including challenging targets. The consistently high yields from this transient CHO platform enabled the delivery of ~20 mg of crude material per 100 mL scale flask production with a throughput capacity of nineteen constructs in a single run. Secondly, we established a magnetic ProA bead in-culture antibody-capturing process, which significantly shortened the production timeline by eliminating the steps of cell centrifugation, filtration, and medium column loading. Third, we utilized the GenScript AmMag™ SA Plus semi-automation, which could handle magnetic ProA bead elution for 12 constructs within less than 1 h. Lastly, we transformed the AKTA Pure^TM system into an automated buffer exchange purification system with a capacity of processing 19 samples in a single run. Results and Conclusions: This new production platform was proven to be robust and could be applied for the routine production of antibodies of sufficient quality and quantity in support of cell-based assays and biophysical characterization. Full article

As rail transit systems advance toward greater automation and intelligence, cloud computing technology, with its remarkable scalability and robust data processing capabilities, has been steadily expanding its footprint in this domain. However, the adoption of cloud computing also introduces new safety challenges for train control systems. Traditional safety computers in train control systems rely on heterogeneous redundancy with symmetry to enhance safety. Nevertheless, the software in cloud computing environments, even if heterogeneous, may share the same source code, thereby triggering the risk of common-cause failures in the software. To address these issues, this study proposes a multi-level monitoring architecture system tailored to the characteristics of cloud-based safety computing platforms. This architecture innovatively integrates the three-level monitoring architecture pattern from the automotive field, the secure channel pattern, and the distributed safety mechanism architecture. It monitors the levels of common-cause software failures that cannot be eliminated through heterogeneity. The introduction of multi-level active monitoring for risk control has reduced the impact of common-cause software failures on system security. By constructing a formal security model, quantitative evaluations are conducted separately on the single-channel L2 and L3, the dual-channel L4 without degradation or monitoring, and the dual-channel L4 monitoring architecture with complete functions. This verifies the effectiveness of the proposed monitoring architecture in reducing the risk of common-cause software failures in the virtualization layer. This study provides a robust theoretical foundation and technical support for the security-oriented design and development of the next-generation intelligent rail transit systems. Full article

Decentralized energy trading has been designed as a scalable substitute for traditional electricity markets. While blockchain technology facilitates efficient transparency and automation for peer-to-peer energy trading, the majority of current proposals lack real-time intelligence and adaptability concerning pricing strategies. This paper presents an innovative machine learning-driven solar energy trading platform on the Ethereum blockchain that uniquely integrates Bayesian-optimized XGBoost models with dynamic pricing mechanisms inherently incorporated within smart contracts. The principal innovation resides in the real-time amalgamation of meteorological data via Chainlink oracles with machine learning-enhanced price optimization, thereby establishing an adaptive system that autonomously responds to fluctuations in supply and demand. In contrast to existing static pricing methodologies, our framework introduces a multi-faceted dynamic pricing model that encompasses peak-hour adjustments, prediction confidence weighting, and weather-influenced corrections. The system dynamically establishes energy prices predicated on real-time supply–demand forecasts through the implementation of role-based access control, cryptographic hash functions, and ongoing integration of meteorological and machine learning data. Utilizing real-world meteorological data from La Trobe University’s UNISOLAR dataset, the Bayesian-optimized XGBoost model attains a remarkable prediction accuracy of 97.45% while facilitating low-latency price updates at 30 min intervals. The proposed system delivers robust transaction validation, secure offer creation, and scalable dynamic pricing through the seamless amalgamation of off-chain machine learning inference with on-chain smart contract execution, thereby providing a validated platform for trustless, real-time, and intelligent decentralized energy markets that effectively address the disparity between theoretical blockchain energy trading and practical implementation needs. Full article

Wireless Sensor Networks (WSNs) have emerged as a foundational technology for monitoring and data collection in diverse domains such as environmental sensing, smart agriculture, and industrial automation. Precise node localization plays a vital role in WSNs, enabling effective data interpretation, reliable routing, and spatial context awareness. The challenge intensifies in range-free settings, where a lack of direct distance data demands efficient indirect estimation methods, particularly in large-scale, energy-constrained deployments. This work proposes a hybrid localization framework that integrates the distance vector-hop (DV-Hop) range-free localization algorithm with the Fire Hawk Optimizer (FHO), a nature-inspired metaheuristic method inspired by the predatory behavior of fire hawks. The proposed FHODV-Hop method enhances location estimation accuracy while maintaining low computational overhead by inserting the FHO into the third stage of the DV-Hop algorithm. Extensive simulations are conducted on multiple topologies, including random, circular, square-grid, and S-shaped, under various network parameters such as node densities, anchor rates, population sizes, and communication ranges. The results show that the proposed FHODV-Hop model achieves competitive performance in Average Localization Error (ALE), localization ratio, convergence behavior, computational, and runtime efficiency. Specifically, FHODV-Hop reduces the ALE by up to 35% in random deployments, 25% in circular networks, and nearly 45% in structured square-grid layouts compared to the classical DV-Hop. Even under highly irregular S-shaped conditions, the algorithm achieves around 20% improvement. Furthermore, convergence speed is accelerated by approximately 25%, and computational time is reduced by nearly 18%, demonstrating its scalability and practical applicability. Therefore, these results demonstrate that the proposed model offers a promising balance between accuracy and practicality for real-world WSN deployments. Full article

Background: Healthcare 5.0 represents a shift toward intelligent, human-centric care systems. Intensive care units generate vast amounts of data that require real-time decisions, but current decision support systems lack comprehensive frameworks for safe integration of artificial intelligence. Objective: We developed and validated the Learn–Predict–Monitor–Detect–Correct (LPMDC) framework as a methodology for systematic artificial intelligence integration across the critical care workflow. The framework improves predictive analytics, continuous patient monitoring, intelligent alerting, and therapeutic decision support while maintaining essential human clinical oversight. Methods: Framework development employed systematic theoretical modeling integrating Healthcare 5.0 principles, comprehensive literature synthesis covering 2020–2024, clinical workflow analysis across 15 international ICU sites, technology assessment of mature and emerging AI applications, and multi-round expert validation by 24 intensive care physicians and medical informaticists. Each LPMDC phase was designed with specific integration requirements, performance metrics, and safety protocols. Results: LPMDC implementation and aggregated evidence from prior studies demonstrated significant clinical improvements: 30% mortality reduction, 18% ICU length-of-stay decrease (7.5 to 6.1 days), 45% clinician cognitive load reduction, and 85% sepsis bundle compliance improvement. Machine learning algorithms achieved an 80% sensitivity for sepsis prediction three hours before clinical onset, with false-positive rates below 15%. Additional applications demonstrated effectiveness in predicting respiratory failure, preventing cardiovascular crises, and automating ventilator management. Digital twins technology enabled personalized treatment simulations, while the integration of the Internet of Medical Things provided comprehensive patient and environmental surveillance. Implementation challenges were systematically addressed through phased deployment strategies, staff training programs, and regulatory compliance frameworks. Conclusions: The Healthcare 5.0-enabled LPMDC framework provides the first comprehensive theoretical foundation for systematic AI integration in critical care while preserving human oversight and clinical safety. The cyclical five-phase architecture enables processing beyond traditional cognitive limits through continuous feedback loops and system optimization. Clinical validation demonstrates measurable improvements in patient outcomes, operational efficiency, and clinician satisfaction. Future developments incorporating quantum computing, federated learning, and explainable AI technologies offer additional advancement opportunities for next-generation critical care systems. Full article

This paper presents the design, development, and preliminary validation of a haptic virtual reality simulator for Shielded Metal Arc Welding (SMAW) at the Pontificia Universidad Católica de Valparaíso, Chile, aimed at enhancing psychomotor training for mechanical engineering students in line with Industry 4.0 demands. The system integrates Unity 3D, a commercial haptic device, and a custom 3D-printed electrode holder replicating the welding booth, enabling interaction through visual, auditory, and tactile feedback. Thirty students with minimal welding experience and seven experts participated in usability and realism assessments. The results showed that 80% of students perceived motor skill improvement, 60% rated realism as adequate, and 90% preferred hybrid training (simulator + workshop). The prototype was practically implemented at the mechanical engineering school, requiring only a mid-range workstation, the Touch haptic device, and the developed software, demonstrating feasibility in real academic settings. The findings indicate potential to build confidence, support motor coordination, and provide a safe, resource-efficient training environment, while experts emphasized the need for automated feedback and improved haptic fidelity. The modular architecture allows scalability, extension to other welding processes, and adaptation for inclusive education. This prototype demonstrates how locally developed immersive technologies can modernize technical education while promoting sustainability, accessibility, and skill readiness. Full article

Healthcare 5.0 represents the next evolution in intelligent and interconnected healthcare systems, leveraging emerging technologies such as Artificial Intelligence (AI) and the Internet of Medical Things (IoMT) to enhance patient care and automation. While Intrusion Detection Systems (IDSs) are a critical component for securing these environments, the current literature lacks a systematic analysis that jointly evaluates the effectiveness of AI models, the suitability of datasets, and the role of Explainable Artificial Intelligence (XAI) in the Healthcare 5.0 landscape. To fill this gap, this survey provides a comprehensive review of IDSs for Healthcare 5.0, analyzing state-of-the-art approaches and available datasets. Furthermore, a practical case study is presented, demonstrating that the fusion of network and biomedical features significantly improves threat detection, with physiological signals proving crucial for identifying complex attacks like spoofing. The primary contribution is therefore an integrated analysis that bridges the gap between cybersecurity theory and clinical practice, offering a guide for researchers and practitioners aiming to develop more secure, transparent, and patient-centric systems. Full article

The integration of AI-driven optimization into Electronic Design Automation (EDA) enables smarter and more adaptive circuit design, where symmetry and asymmetry play key roles in balancing performance, robustness, and manufacturability. This work presents a model-driven optimization methodology for sizing low-phase-noise LC voltage-controlled oscillators (VCOs) at 5 GHz, targeting Wi-Fi, 5G, and automotive radar applications. The approach uses the EKV transistor model for analytical CMOS device characterization and applies a diverse set of metaheuristic algorithms for both single-objective (phase noise minimization) and multi-objective (joint phase noise and power) optimization. A central focus is on how symmetry—embedded in the complementary cross-coupled LC-VCO topology—and asymmetry—introduced by parasitics, mismatch, and layout constraints—affect optimization outcomes. The methodology implicitly captures these effects during simulation-based optimization, enabling design-space exploration that is both symmetry-aware and robust to unavoidable asymmetries. Implemented in CMOS 180 nm technology, the approach delivers designs with improved phase noise and power efficiency while ensuring manufacturability. Yield analysis confirms that integrating symmetry considerations into metaheuristic-based optimization enhances performance predictability and resilience to process variations, offering a scalable, AI-aligned solution for high-performance analog circuit design within EDA workflows. Full article

With the increasing integration of mobile technologies into manufacturing automation environments, the effective visualisation of data on small-screen devices has emerged as an important consideration. This study investigates the usability and readability of common visualisation types (bar charts, line charts, and tables) on mobile devices, comparing different interface designs and interaction methods. Using a within-subject experimental design with 11 participants, we evaluated two primary approaches for handling large visualisations on mobile screens: segmented (cutting) displays versus continuous (dragging) displays. Results indicate that segmented displays generally improve task completion time and reduce mental workload for bar charts and tables. In contrast, line charts exhibit more complex patterns that depend on the size of the data. These findings provide practical guidelines for designing responsive data visualisations optimised for mobile interfaces. Full article

Population growth drives increasing energy demands, agricultural production, and organic waste generation. The organic waste contributes to greenhouse gas emissions and increasing landfill burdens, highlighting the need for novel closed-loop technologies that integrate water, energy, and food resources. Within the context of the Water–energy–food Nexus (WEF), wastewater can be recycled for food production and food waste can be converted into clean energy, both contributing to environmental impact reduction and resource sustainability. A novel household-scale, closed-loop WEF system was designed, installed and operated to manage organic waste while retrieving water for irrigation, nutrients for plant growth, and biogas for energy generation. The system included a biodigester for energy production, a sand filter system to regulate nutrient levels in the effluent, and a hydroponic setup for growing food crops using the nutrient-rich effluent. These components are operated with a daily batch feeder coupled with automated sensors to monitor effluent flow from the biodigester, sand filter system, and the feeder to the hydroponic system. This novel system was operated continuously for two months using typical household waste composition. Controlled experimental tests were conducted weekly to measure the nutrient content of the effluent at four locations and to analyze the composition of biogas. Gas chromatography was used to analyze biogas composition, while test strips and In-Situ Aqua Troll Multi-Parameter Water Quality Sonde were employed for water quality measurements during the experimental study. Experimental results showed that the system consistently produced biogas with 76.7% (±5.2%) methane, while effluent analysis confirmed its potential as a nutrient source with average concentrations of phosphate (20 mg/L), nitrate (26 mg/L), and nitrite (5 mg/L). These nutrient values indicate suitability for hydroponic crop growth and reduced reliance on synthetic fertilizers. This novel system represents a significant step toward integrating waste management, energy production, and food cultivation at the source, in this case, the household. Full article

Background/Objectives: Neuropsychological assessments are valuable tools for evaluating the cognitive performance of older adults. Limitations associated with these in-person paper-and-pencil tests have inspired efforts to develop digital assessments, which would expand access to cognitive screening. Digital tests, however, often lack validity relative to gold-standard paper-and-pencil versions that have been robustly validated. Speech-to-text (STT) technology has the potential to improve the validity of digital tests through its ability to capture verbal responses, yet the effect of its performance on standardized scores used for cognitive characterization is unknown. Methods: The present study evaluated the accuracy of Apple’s STT engine relative to ground-truth transcriptions (RQ1), as well as the effect of the engine’s transcription errors on resulting standardized scores (RQ2). Our study analyzed data from 223 older adults who completed a digital assessment on an iPad that used STT to transcribe and score task responses. These automated transcriptions were then compared against ground-truth transcriptions that were human-corrected via external recordings. Results: Results showed differences between STT and ground-truth transcriptions (RQ1). Nevertheless, these differences were not large enough to practically affect standardized measures of cognitive performance (RQ2). Conclusions: Our results demonstrate the practical utility of Apple’s STT engine for digital neuropsychological assessment and cognitive characterization. These findings support the possibility that speech-to-text, with its ability to capture and process verbal responses, will be a viable tool for increasing the validity of digital neuropsychological assessments. Full article