Search Results (589)

Background: Malignant pleural mesothelioma (MPM) is an aggressive asbestos-associated cancer in which inflammatory pathways may contribute to tumor behavior. NF-κB signaling is a central mediator of inflammation and cell survival, but the relative contributions of its associated enzymes, IKKα- and IKKβ, in MPM models remain incompletely understood. Methods: We characterized the involvement of IKKα- and IKKβ in tumor formation using several in vitro approaches and the intrapleural or subcutaneous delivery of three murine MPM cell models (AB2, AE17 and KPM1). The experimental approaches included shRNA silencing of Chuk and Ikbkb—the genes encoding the activity of IKKα- and IKKβ—and tumor growth, apoptosis and colony formation measurements. Exploratory pathway and transcriptional analyses were carried out following RNA-sequencing of our murine cell lines and a publicly available human gene expression dataset was also analyzed to provide complementary evidence. Results: Activation of the IKKα- and IKKβ was confirmed in the AB2, AE17 and KPM1 cell lines. Silencing both Chuk and Ikbkb decreased target and cross-target transcripts and metabolic growth. Importantly, Chuk silencing was associated with increased early apoptotic staining in AE17 and KPM1 cells, increased late apoptotic staining in AE17 cells and reduced colony formation in all cell models. Furthermore, Chuk-associated effects on tumor burden and activity were more notable than Ikbkb-associated effects in both intrapleural and subcutaneous tumor formation parameters. Finally, while canonical NF-κB gene-set clustering in both human and murine tumor samples identified specific and shared pathways and transcriptional profiles supporting the in vitro murine assay findings, the analyses remained exploratory and did not specifically establish target dependency. Conclusions: Our data support the involvement of IKK/NF-κB-associated signaling in cell survival, clonogenicity and tumor growth in several MPM murine cell models and identify Chuk-related signaling as a promising target in future studies. Full article

Drinking water is essential for humans; its quality determines the health and proper functioning of the body. In order to obtain drinking water in accordance with the legislation in force, the performance of the technology applied for water treatment and the infrastructure for transport and distribution to the consumer are equally important. Particular importance to this last aspect was given by the introduction of European Directive 2020/2184. Infrastructure can significantly influence water quality due to the fact that there are periods when water stagnates in the pipe and electro-corrosion processes, and salt deposits or degradation/corrosion of the pipes can occur, or periods when water circulates under pressure, when deposits but also particles from the pipes are mechanically detached and transported to the consumer. In this article, we aim to present the behavior of asbestos-cement pipes, AC, special steel for pipes OLT 37, galvanized steel, OLT 37.1, and high-density polyethylene, HDPE, in contact with drinking water. The study was conducted in Calarasi city, Calarasi county, Romania, where some of the old pipes were replaced. A comparative study of new pipes of the same type with pipes in use for more than 20 years, up to 46 years, was conducted, and at the same time the changes that the pipes (which have not yet been replaced) have on the quality of drinking water for street consumers were also analyzed. Following the analyses performed, it can be stated that the water quality is within the maximum permissible limits, but the micropollutants that appear can accumulate in the body (such as AC microfibers, microplastics, metallic zinc, zinc ions, iron ions, manganese, aluminum) with effects that can be evident after a long period of consumption through bioaccumulation. Full article

Mesothelioma is a rare and aggressive malignancy arising from mesothelial cells. Despite recent advances in systemic therapies, overall survival remains limited, underscoring the need for a deeper mechanistic understanding of disease pathogenesis and novel therapeutic strategies. In mesothelioma, chronic tissue injury induced by asbestos fibers leads to sustained activation of innate immune pathways and chronic inflammation that actively promote tumorigenesis. The release of Damage-Associated Molecular Patterns (DAMPs)—endogenous molecules that signal cellular stress and damage—contributes to establishing a self-sustaining inflammatory circuit within the pleural microenvironment that promotes tumor initiation and progression and immune evasion. Among DAMPs, High-Mobility Group Box 1 (HMGB1) has emerged as a key regulator of mesothelioma pathogenesis. Several studies demonstrated that mesothelial cells actively secrete HMGB1 in response to asbestos exposure, driving macrophage recruitment, cytokine production, and chronic inflammation. Beyond HMGB1, additional DAMPs—including IL-33, extracellular ATP, cell-free nucleic acids, heat shock proteins, and calreticulin—contribute to inflammasome activation, stromal remodeling, and immune dysregulation. Recent evidence suggests that DAMP signaling in mesothelioma is dysregulated, resulting in chronic inflammation coupled with ineffective antitumor immunity. This review provides a comprehensive synthesis of DAMP biology in mesothelioma, highlighting the emerging therapeutic opportunities targeting DAMP-associated pathways. Full article

Long utility records support renewal screening when failures, durations and exposure use consistent definitions. An audit covered 4885 water-supply failures in a major city in south-eastern Poland (2004–2025); 4859 had a valid repair duration and 4832 had a valid service-interruption status/duration. Events and annual exposure used the same diameter-based functional classification. Material was identified for 4824 events; 4806 events formed 14 material–function groups. The core CRITIC–TOPSIS model combined exposure-normalised failure rate, mean repair time and mean interruption time; 2000 event-level bootstrap replicates quantified sampling uncertainty. Network length increased by about 84.4%, while failure rate decreased (Spearman ρ = −0.911, p < 0.001). Mean repair and interruption times were 7.96 and 2.43 h; 64.2% of repairs equalled 8 h. Galvanised-steel service connections ranked first (CC = 0.819; P(rank 1) = 0.800; P(top 3) = 1.000), followed by steel and asbestos cement distribution pipes. Product-containing structures and the strict >8, >10 and >12 h threshold variants selected asbestos cement distribution pipes, whereas the inclusive ≥8 h and positive-only SIT variants retained galvanised-steel service connections. The result is a group-screening signal, not a renewal prescription; hydraulic consequence, customers affected, condition and cost remain necessary for segment decisions. Beyond the case study, the workflow provides a transferable, low-data screening approach for directing limited maintenance and renewal resources while accounting for water-service accessibility; numerical priorities should be recalculated using local exposure, operational conditions and decision thresholds. Full article

Demolition is recognised as a strategic construction process enabling the transition between an asset and a building cycle. Rather than removing a structure, it requires a design and management approach that preserves safety, material quality, traceability, and environmental performance. This paper develops a demolition-management framework linking pre-demolition assessment, method selection, hazardous-material control, waste classification, logistics, safety measures, environmental mitigation, and documentation-based traceability. The framework is applied to the partial demolition of a precast reinforced-concrete storage building within an industrial compound in Treviso, Italy. The project involved time constraints, limited working space, adjacent structures, and asbestos–cement components. The case study illustrates a phased process including asbestos abatement, perimeter slab cutting, crane-assisted dismantling, controlled top-down mechanical demolition, dust suppression, and coordinated temporary storage and waste transport documentation. Results show that safety and circular-economy objectives are mutually reinforcing when demolition is planned as an integrated and auditable end-of-life construction-management process, focused on removing, separating, classifying, and routing materials through pathways. In constrained industrial settings, sequencing, staging areas, specialised subcontracting, and documentation readiness are decisive for reducing interference, maintaining operational continuity, and supporting material recovery. The study proposes a workflow, while acknowledging the limitations of a single-case design and the absence of multi-project quantitative data. Full article

(1) Background: Indonesia is one of the world’s largest consumers of asbestos, with widespread use of asbestos-cement roofing. Java Island, characterized by high population density and seismic vulnerability, presents a complex risk landscape for asbestos exposure. This study aimed to develop a district-level asbestos exposure risk map for Java by integrating asbestos roof prevalence, population density, and seismic hazard. (2) Methods: A descriptive, semi-quantitative approach was applied using secondary data from national statistics and geological hazard portals. Three variables—asbestos roof prevalence, population density, and seismic hazard—were categorized into five ordinal levels (very low to very high) and combined using GIS-based risk matrices to generate composite exposure risk maps. Sensitivity and statistical analyses were conducted to assess the robustness and internal consistency of the risk classification. (3) Results: Of 119 districts/municipalities, 43 (36%) were classified as very high risk and 45 (38%) as high risk under the primary GIS-based risk matrix classification. Major metropolitan corridors, including Greater Jakarta and Bandung, showed elevated risk where substantial asbestos use coincided with very high population density, whereas several southern and inland districts gained priority because of higher seismic hazard. Sensitivity analyses indicated that the classification was generally robust to moderate changes in weighting and threshold boundaries, although the predefined population density thresholds provided limited discrimination within Java. (4) Conclusions: Asbestos exposure risk in Java is both widespread and heterogeneous, shaped by the intersection of infrastructure, demographics, and seismic vulnerability. The map offers a practical tool for prioritizing asbestos control, public awareness campaigns, health surveillance, and disaster risk reduction measures in Indonesia. Full article

Background: While live-cell therapies face significant translational hurdles, cell-free secretomes derived from fetal progenitor cells offer a unique, scalable approach natively programmed for scarless tissue repair. Methods: This GLP-compliant study evaluated the therapeutic efficacy spectrum of an off-the-shelf, clinical-grade, intratracheal lyophilized FE002-Lu lung fibroblast conditioned medium (LFCM) across five controlled Wistar rat models of induced lung injury: bleomycin (5 mg/kg), asbestos (100 µg/rat), hyperoxia (100% oxygen exposure), silica (30 mg/rat), and lipopolysaccharide (LPS, 4 mg/kg). Following lung injury induction and symptom onset, symptomatic rats were randomized to receive intratracheal LFCM (low, mid, or high dose) or a vehicle control every 4 days for 28 days. Results: The intervention demonstrated an exceptional safety profile, maintaining 100% survival with no severe procedural toxicity across all cohorts. Across all study arms, LFCM effectively attenuated pulmonary inflammation, reducing pro-inflammatory markers (IL-1β, IL-6, TNF-α, and CINC-1) in bronchoalveolar lavage fluid. Concurrently, high LFCM doses consistently elevated the anti-inflammatory cytokine IL-10. Within the lung tissue, mid- and high doses significantly reduced key pro-fibrotic drivers, including TGF-β1, TIMP-1, WISP-1, and hydroxyproline. Treatments consistently decreased α-SMA expression and pro-fibrotic gene mRNA levels, mitigating pulmonary myofibroblast activation. This correlated with reduced Ashcroft scores and collagen deposition, thereby preserving lung architecture. Notably, the FE002-Lu LFCM treatment exerted a biphasic regulation of extracellular matrix turnover: it elevated Cathepsin-D and MMP-12 in the bleomycin arm to actively clear newly deposited fibrotic debris, while reducing these markers in the hyperoxia and LPS arms to prevent acute collateral degradation of the native lung matrix. In both particulate models (asbestos and silica), the LFCM mid-dose established an optimal therapeutic threshold, avoiding the localized secretome saturation and pro-fibrotic exacerbation occasionally observed at higher doses. Conclusions: Lyophilized FE002-Lu LFCM acts as a potent, pleiotropic biologic that effectively resolves acute pulmonary inflammation and arrests progressive fibrotic remodeling across multiple distinct in vivo models. By overcoming the cold-chain and delivery limitations inherent to pulmonary live-cell therapies, this stable, cell-free secretome represents a highly scalable, “off-the-shelf” candidate poised for non-invasive, aerosolized clinical translation. Full article

This article examines the Carbon Border Adjustment Mechanism (CBAM) as a regulatory instrument of the European Union, situated at the intersection of international trade law, customs law and environmental protection. The primary objective of the study is to assess the legal nature of the CBAM, evaluate its compatibility with World Trade Organisation (WTO) law and analyse its implications for sustainable global trade governance and climate policy. Particular attention is paid to Articles I, III and XX of the GATT, as well as relevant WTO case law, including the cases of United States—Gasoline, United States—Shrimp, European Communities—Asbestos and Brazil—Retreaded Tyres. The study further examines the procedural transformation of customs authorities, particularly in relation to the verification of emissions, the burden of proof and administrative enforcement. The article also assesses the environmental effectiveness of the CBAM in preventing carbon leakage and in enforcing higher environmental standards in third countries, addressing the legal and economic issues of discrimination, proportionality, administrative complexity and potential impact on developing economies. The research is based on doctrinal legal analysis, comparative legal methodology and a review of relevant international and European legal sources, including WTO case law and Regulation (EU) 2023/956. The authors demonstrate that the CBAM represents a hybrid regulatory mechanism combining elements of environmental taxation, regulatory charges and trade policy instruments implemented through customs procedures. The originality of the article lies in its integrated and comprehensive analysis of the CBAM from the perspective of customs law, international trade law and environmental regulation with a focus on sustainability. In contrast to existing studies focusing primarily on trade or climate aspects, the article highlights the evolving regulatory role of customs authorities in the framework of European Union climate governance. The findings suggest that the CBAM is generally compatible with WTO law, provided that it is implemented in a transparent, proportionate, flexible and non-discriminatory manner. The authors conclude that the CBAM can become an important model for the future development of sustainable international economic regulation and climate-related trade governance. Full article

Asbestos contamination in drinking water remains an underexplored environmental issue despite the widespread historical use of asbestos-cement materials in water infrastructure and buildings worldwide. This study evaluated the occurrence and removal of asbestos fibers throughout the drinking water treatment chain in Cartagena de Indias, Colombia, from source contamination to household filtration. Synthetic asbestos-contaminated water prepared from asbestos-cement roofing materials was treated using coagulation–flocculation with aluminum sulfate, a pilot-scale conventional treatment train, membrane filtration, and household point-of-use filtration systems. Asbestos quantification and mineral identification were performed using transmission electron microscopy (TEM), with results reported as million fibers per liter (MFL). Coagulation–flocculation achieved the highest removal efficiencies, reaching up to 99.8% at aluminum sulfate dosages between 35 and 45 mg/L. The pilot-scale treatment system substantially reduced asbestos concentrations, with granular filtration representing the principal removal barrier. Membrane filtration exhibited moderate and highly variable performance, with an average removal efficiency of 44.7%, whereas household filters showed inconsistent behavior and occasional concentration peaks associated with possible breakthrough or remobilization processes. The results demonstrate that asbestos removal is strongly governed by the treatment mechanism, with particle destabilization and aggregation outperforming technologies relying exclusively on physical retention. These findings support the progressive replacement of asbestos-containing water infrastructure as the primary long-term preventive strategy, complemented by targeted monitoring and optimized asbestos-removal treatment where fiber contamination is detected or a risk of release from legacy materials exists. Full article

Background/Objectives: Sex-based differences in asbestos exposure and tumor biology in pleural mesothelioma (PM) remain incompletely characterized. As an expert tertiary center, we sought to systematically characterize asbestos exposure by sex and assess biomarker prognostic relevance in surgically treated pleural mesothelioma. Methods: We conducted a retrospective, single-center cohort encompassing all patients who underwent surgical intervention for PM at our institution. Demographic characteristics, asbestos exposure history, tumor biomarker profiles, and survival outcomes were extracted from this database and supplemented by review of the electronic medical record. Sex differences in asbestos exposure and biomarker expression were assessed using Fisher’s exact test on an available-case basis, while logistic regression was used to identify predictors of documented asbestos exposure. Overall survival was estimated using Kaplan–Meier analysis; independent associations between biomarkers and mortality were determined via multivariable Cox proportional hazards models adjusted for age, sex, and histological subtype. Results: Between 2015 and 2024, 106 patients (75% males) underwent surgical intervention for PM, with a median follow-up of 4.4 years. The mean age at surgery was 67.5 years (Standard Deviation [SD], 11). The cohort predominantly consisted of patients with epithelioid histology (n = 86, 81%), followed by biphasic (n = 16, 15%), and sarcomatoid (n = 4, 4%) subtypes. Thirty (28.3%) were alive at last follow-up. Median overall survival was 15.6 months (95% Confidence Interval [CI], 13.8–25.4 months), and 5-year survival was 17.8%. Of 92 patients with available exposure data, 66 (72%) had documented exposure histories. Although most of these exposures were occupational (46, 78%), a striking disparity was observed between occupational exposures in males vs. females (44 [86%] vs. 2 [25%]; n = 8 classifiable females; p < 0.001). Multivariable analysis demonstrated that male sex was associated with increased odds of asbestos exposure (Adjusted Odds Ratio [OR_adj], 5.45; 95% Confidence Interval [CI], 1.90–16.35). PD-L1, Ki-67, or BAP1 expression were not associated with sex, age, or asbestos exposure. High Ki-67 (>15%) and PD-L1 (>15%) were associated with a four-fold and three-fold increased risk of mortality (Hazard Ratio_adj [HR_adj], 4.33 [95% CI, 1.85–10.13] and HR_adj, 2.80 [95% CI, 1.39–5.64], respectively), whereas BAP1 loss was not (HR_adj, 0.80; 95% CI, 0.45–1.44). Conclusions: Our findings underscore the potential value of sex-sensitive screening protocols that more comprehensively assess non-occupational asbestos exposure pathways and offer additional evidence regarding the prognostic relevance of PD-L1, Ki-67, and BAP1 in surgical patients. Full article

To improve the seismic performance and post-earthquake recoverability of low- and mid-rise steel frames, this study investigates the seismic performance and layout strategy of a self-centering friction damper (SCFD) through experimental and numerical studies. The SCFD combines the superelastic restoring capability of shape memory alloy (SMA) bars with the energy dissipation provided by non-asbestos organic (NAO) friction materials. Monotonic and cyclic tests were conducted to characterize the mechanical behavior of Ni–50.8 at. % Ti SMA bars and the hysteretic performance of the SCFD, based on which a numerical model of the damper was established and validated. An uncontrolled frame and four controlled frames employing diagonal, chevron, improved lower toggle-brace, and improved upper toggle-brace layouts were comparatively investigated to evaluate the effects of brace configuration, installation position, and damper quantity on seismic performance. The proposed damper exhibited an equivalent damping ratio ranging from 24% to 32%. When the SMA strain exceeded 6%, the residual deformation of the damper increased significantly, indicating that excessive SMA deformation should be avoided in practical design. Among the investigated configurations, the improved upper toggle-brace layout, combined with additional dampers installed at the first story, showed the best overall performance. Compared with the uncontrolled multi-story structure, the residual inter-story drift ratio was reduced by 76.7–93.5%, while the maximum acceleration reduction reached 28.9%. However, local acceleration amplification was observed in some cases because of the increased structural stiffness. These findings provide practical guidance for the layout design and engineering application of self-centering friction dampers in low- and mid-rise steel frames. Full article

Asbestos exposure remains an important cause of lung cancer and mesothelioma worldwide, and the long latency of asbestos-related diseases means that health and economic consequences persist even after asbestos bans. In Colombia, the economic burden attributable to historical asbestos exposure has not been comprehensively quantified. This study estimated the economic burden attributable to asbestos exposure in Colombia in 2022 using a prevalence-based cost-of-illness framework from a societal perspective. Direct medical costs were derived from a national lung cancer cost study and updated to 2022 values. Epidemiological inputs were obtained from the Global Burden of Disease (GBD) database. Mesothelioma was considered predominantly attributable to asbestos exposure, while lung cancer was adjusted using population-attributable fractions and an alternative 1:3 mesothelioma-to-lung-cancer ratio scenario. Indirect costs were estimated using the human capital approach, and disability-adjusted life year (DALY) losses were monetized using alternative willingness-to-pay thresholds. Colombia was estimated to have 150 prevalent mesothelioma cases and between 389 and 450 asbestos-attributable lung cancer cases in 2022. Direct costs ranged from USD 6.5 to 10.7 million annually, increasing to USD 16.7–23.1 million when productivity losses were included. Expanded societal valuation scenarios ranged from USD 71 million to more than USD 180 million annually. Asbestos-related malignancies continue to impose a substantial economic burden in Colombia and support the need for strengthened surveillance, long-term management of installed asbestos, and policies aimed at reducing future asbestos-related disease burden. Full article

Background/Objective: This study aimed to develop and validate an automated framework for detecting pleural plaques (PPs) at the patient level using a single chest CT scan. Methods: A database of chest CT scans with corresponding individual binary annotations for PP presence was first established through expert visual assessment, based on consensus among a board of specialized radiologists. This dataset served to train and validate a patient-level classification framework for automated PP presence detection. This framework leverages a pre-trained PP segmentation network, thus obviating the need for retraining large-scale deep learning models. This segmentation backbone is supplemented by a lightweight classification module that integrates the network’s outputs to infer the presence or absence of PPs at patient level. Furthermore, a patch-wise processing strategy was employed to optimize computational efficiency and training time while retaining critical local contextual information. Results: The framework was evaluated on a cohort of 1241 retired workers with documented occupational asbestos exposure, using 10-fold cross-validation (30% training, 10% validation, 60% testing). It achieved a balanced accuracy of 97.0%, sensitivity of 96.6%, and specificity of 97.4%, demonstrating performance slightly superior to that of two expert radiologists (balanced accuracy: 90.9%/91.4%, sensitivity: 88.0%/88.8%, specificity: 93.9%/94.1% for Expert1/Expert2) and substantially superior to that of a radiologist without specialized expertise in asbestos-related manifestations (balanced accuracy: 80.0%, sensitivity: 75.1%, specificity: 84.8%). Conclusion: The proposed framework demonstrates strong potential for automated patient-level identification of pleural plaques from a single CT scan. Our approach could be particularly useful in screening settings that involve compensation claims related to occupational diseases. Full article

The development of sustainable, high-performance friction composites is critical for the automotive industry, given the environmental and health concerns associated with conventional brake pad materials such as asbestos and copper. This study investigates the effect of incorporating micro- and nano-sized Al₂O₃, SiC, and carbon nanotubes (CNTs) into a novel, eco-friendly, asbestos-free, and copper-free brake pad formulation. Six composite samples were fabricated via a cold-pressing and hot-molding process: five formulations containing a single, size-controlled micro-/nano-sized reinforcement (Al₂O₃, SiC, and CNTs), and one reference formulation (CBP# Reference) containing an unrefined, commercial-grade combination of Al₂O₃ and SiC in place of the size-controlled additive. All formulations were rigorously characterized for their physical, mechanical, and tribological properties. The nano-Al₂O₃ formulation exhibited the highest density (2.197 g/cm³) and compressive strength (249.7 MPa), while the micro-SiC formulation achieved superior wear resistance, recording the lowest weight loss (0.0053 g) and the highest hardness (90 HV). The nano-SiC formulation offered the most balanced overall performance, combining high hardness (86.2 HV) with the highest average friction force (33.65 N) and the most stable friction-time response among all samples. The CNT-reinforced formulation produced the highest maximum friction force (42.07 N) but showed only moderate improvement in density, hardness, and compressive strength relative to the ceramic-reinforced samples. Compared with the CBP# reference, all five developed formulations exhibited higher hardness and coefficient of friction alongside lower weight loss, confirming their potential as durable, sustainable alternatives for automotive brake friction applications. Full article