Search Results (2,590)

Clinoptilolite is a zeolite with a microporous structure that enables ion exchange, molecular sieving, and adsorption, conferring detoxifying, antioxidant, and anti-inflammatory properties. These properties have applications in food, medicine, catalysis, and environmental remediation. This study evaluated the safety of the lab-made Clinoptilolite as a potential food ingredient through a 90-day repeated-dose toxicity study in male and female Sprague Dawley rats. The test substance was administered via oral gavage at doses of 0, 5, 10, and 15 mg/kg bw/day, followed by a 28-day recovery period. In addition, genotoxicity was assessed using the Ames test, in vitro chromosomal aberration assay, and an in vivo micronucleus test. All studies were conducted in accordance with OECD and FDA guidelines. Results showed no adverse systemic, genotoxic, or irreversible effects at any dose, with minor clinical variations being incidental and reversible. Genotoxicity tests confirmed no mutagenic or clastogenic potential. Overall, the lab-made Clinoptilolite evaluated in this investigation was well tolerated, non-toxic, and showed no evidence of treatment-related toxicity at the doses tested. These findings provide supportive evidence for its consideration toward a Generally Recognized as Safe (GRAS) determination. Full article

Background: We are developing cytotoxic immunoconjugates (CICs) to eliminate HIV-infected cells. We investigated the efficacy and kinetics of killing by different forms of CICs targeted by the same monoclonal antibody (mAb), an immunotoxin (IT), antibody-drug conjugate (ADC), and radioimmunoconjugate (RIC). Methods: We compared in vitro effects of CICs made by conjugating anti-gp41 mAb 7B2 to deglycosylated ricin A chain (7B2-dgA), the anthracycline derivative PNU-159682 (7B2-PNU), or the α-emitting isotope actinium-225 (7B2-²²⁵Ac). Kinetic analyses of cell growth were performed measuring electrical impedance every 15 min over a 7-day period using cells stably expressing the HIV envelope and Env-negative parent cells. Results: 7B2-dgA and 7B2-²²⁵Ac were more potent and acted more rapidly to kill cells than 7B2-PNU. Both the 7B2-PNU and 7B2-²²⁵Ac induced bystander-cell killing, whereas the IT did not and consequently allowed the outgrowth of Env-negative cells. Low dose or brief exposure to 7B2-PNU resulted in an increased rate of cell growth. Conclusions: An IT, ADC, and RIC showed substantial differences in the degree of specific toxicity, kinetics, and mechanisms of killing. The results of this side-by-side comparison have implications for the development of CICs to treat HIV, as well as other conditions. Full article

This research examines the impact of positive crude oil revenue shocks on Russia’s macroeconomic policy and economic development, analyzes the effects of macroeconomic policy on the economy, and compares these effects across two subsamples (2005–2013 and 2015–2019). The study proves that the full 2005–2019 model fails to capture the transmission responses of policy and macroeconomic variables after the significant structural shift in the post-2014 period, while subsample models each provide a better fit and more accurate results. Our empirical research provides the following insights: First, after 2014, fiscal expansion shifted from an anti-inflationary tool to an inflationary driver as well as a depreciating force on the national currency. Second, after 2014 the monetary policy’s tight stance became explicitly anti-inflationary compared with its direct opposite effects before 2014. Third, after 2014, the central bank’s more dominant inflation-targeting regime tightened the constraints on fiscal policy. Fourth, the Russian Federation’s economic dependence on oil diminished after 2014. Finally, macroeconomic policy (government expenditure and key interest rate) shifted from procyclical to countercyclical in response to oil revenue shocks after 2014. Full article

Background: Leptospirosis is an important zoonosis that can present as a self-limited influenza-like illness or progress to severe, including life-threatening multiorgan dysfunction. We report the epidemiology, clinical profile, and correlates of severity among adults hospitalized patients with leptospirosis diagnosed in central Romania over a period of 17 years. Methods: We conducted a retrospective, single-center cohort study of adults admitted between 1 January 2008 and 1 December 2025 with laboratory-confirmed leptospirosis. Confirmation was based on positive anti-Leptospira IgM serology, with repeat testing when the initial result was equivocal and confirmation with a microscopic agglutination test. We extracted demographic, exposure, clinical, laboratory, treatment, and outcome data from medical records. The modified Faine score was also calculated using admission data. Results: Sixty-four patients were included in this analysis, of which 53 (82.8%) were male patients. Admissions peaked in 2023–2025 (34/64, 53.1%) and in the August–September months. Reported exposures were predominantly peri-domestic (46.9%), followed by rural/animal-related occupations (20.3%) and freshwater contact (17.2%). Severe disease occurred in 26/64 (40.6%), was more frequent in men (p = 0.021), and was more common pre-pandemic than during/after the pandemic (p < 0.001). Severe cases were associated with oliguria/anuria, hematuria, and jaundice, alongside higher urea/creatinine and bilirubin, lower hemoglobin and lymphocyte percentages, and a longer hospitalization period. One in-hospital death occurred (1.6%). Serogroup identification was available for 10 patients (15.6%) (pre-pandemic only). The mean modified Faine score was 27.5 ± 6.0. Conclusions: In this temperate-region cohort study, hospitalized leptospirosis showed a marked male predominance, a late-summer peak, and a substantial burden of severe disease. Early renal and hepatobiliary manifestations with concordant laboratory abnormalities may support timely risk stratification and escalation of care, while expanded molecular diagnostics and systematic typing are needed to clarify temporal trends and guide prevention. Full article

Heat waves (HWs), characterized by periods of unusually high temperature, would inevitably affect the soil microenvironment and then soil respiration (Rs), which is considered to be the most active part of the global carbon cycle. An in situ spring and summer HWs simulation experiment combined with a locally common human disturbance (mowing) was conducted to separate Rs into autotrophic respiration (Ra) and heterotrophic respiration (Rh) on a natural Eurasian meadow steppe in 2018 and 2019. HWs significantly affected grassland Rs, Rh and Ra (p < 0.01) and also interacted with mowing, but the effect of spring HW and summer HW were different. During the summer HWs, daily Rs of the non-mowed plots increased by 1.07 μmol m⁻² s⁻¹ (11.71%) and increased in the mowed plots by 2.15 μmol m⁻² s⁻¹ (23.81%). During the spring HWs, daily Rs of the non-mowed plots decreased by 0.13 μmol m⁻² s⁻¹ (2.36%) and decreased by 0.52 μmol m⁻² s⁻¹ (9.02%) in the mowed plots. Rs, Rh and Ra were inhibited by spring HWs, but promoted by summer HWs. Our results indicated that the occurring time change in HWs would cause widely divergent influences on the ecosystem, and mowing would decrease the anti-interference ability of the ecosystem, which acted as an enhancement on both the positive and negative effects of HWs. These findings have important implications for accurate model prediction and carbon budget assessment. Full article

The aim of this study was to improve surgical caponisation and identify stress factors associated with different pain management methods and post-operative periods. A total of 72 Prelux-G cockerels were caponised at six weeks of age. Eight experimental groups were established based on caponisation status and pain management. Four groups were caponised using different pain management protocols (none, anaesthesia only, analgesia only, or both), while four non-caponised groups served as controls, including one group that received anaesthesia and analgesia without surgery. The remaining three groups served as non-caponised controls with varying levels of handling. The researchers monitored body weight, feed intake, respiratory and heart rate, vocalisations, response to pain, and depth of anaesthesia. The results confirmed that caponisation is painful, as reflected in increased physiological responses and vocalisations. The use of non-steroidal anti-inflammatory drugs alone did not provide effective pain relief. Additionally, the surgical procedure had no significant effect on weight gain or feed intake during the seven days following surgery. Stress levels, measured by blood serum corticosterone concentration, also increased during the procedure. The study concludes that anaesthesia significantly reduces pain and facilitates surgery, which is essential for improving animal welfare. Full article

Background/Objectives: Growing concerns about antibiotic-associated adverse events, including Clostridioides difficile infection, prompted implementation of an antibiotic stewardship program (ASP) in South Korea in November 2024. One year post-implementation, we evaluated changes in antibiotic consumption and C. difficile infection incidence. Methods: This study was conducted at Chung-Ang University Gwangmyeong Hospital, South Korea. Segmented regression and interrupted time series analyses were performed using weekly data on antibiotic use (days of therapy [DOT] per 1000 patient-days) and C. difficile infection or colonization (cases per 1000 patient-days) over 157 weeks (November 2022–October 2025). Weeks 1–105 defined the pre-ASP period, and weeks 106–157 the post-ASP period. A 4-week lag between antibiotic use and subsequent C. difficile infection was hypothesized. Results: Before ASP, weekly total antibiotic use increased (β₁ = 1.14, 95% CI, 0.76 to 1.51, p < 0.001). After ASP, the slope decreased significantly (β₃ = −1.50, 95% CI −2.62 to −0.39, p = 0.009), consistent across anti-pseudomonal penicillins and cephalosporins and fluoroquinolones. Pre-ASP C. difficile incidence increased (α₁ = 0.01, 95% CI, 0.01 to 0.02, p < 0.001); the upward trend attenuated post-ASP, though slope change was not significant (α₃ = −0.01, 95% CI, −0.03 to 0.004, p = 0.13). An increase of 1 DOT per 1000 patient-days was associated with a 0.005-case increase in C. difficile infection incidence after 4 weeks. Conclusions: The observed effects of proactive ASP strategies underscore the importance of maintaining stewardship in clinical practice. Further studies are warranted to assess the sustainability of these findings and evaluate additional factors influencing C. difficile infection incidence. Full article

Background: In recent years, the sociopolitical landscape in the United States has shifted due to an increase in state-level legislation regarding LGBTQ+ rights, a trend that has been particularly pronounced in the Commonwealth of Kentucky. While the mental health impacts of enacted laws are increasingly documented, a critical gap remains in understanding the psychological toll of the legislative debates themselves—the prolonged periods of public discourse surrounding the restriction of rights. Methods: Utilizing data from the 2025 Queer Kentucky Survey (N = 817), this exploratory study examined the association between LGBTQ+ community belongingness and acute suicidality attributed specifically to anti-LGBTQ+ legislative debates. Data were derived from a non-probability snowball sample. Binary logistic regression models that adjusted for age, race, gender identity, education, and income were utilized. Results: Prevalence of debate-attributed suicidality was alarmingly high: 59.7% of the sample attributed increased suicidal thoughts, and 44.1% attributed a suicide attempt, specifically to the legislative debates. LGBTQ+ belongingness was a robust protective correlate, associated with significantly lower odds of both suicidal thoughts (OR = 0.61, p < 0.001) and attempts (OR = 0.41, p < 0.001). Analyses further revealed divergent risk for suicidality across demographic characteristics. Conclusions: Findings are consistent with the interpretation that legislative debates may function as distinct structural stressors associated with suicidal thoughts and suicide attempts. While community belongingness may offer a critical buffer, the elevated risks among Transgender and Black, Indigenous, and People of Color (BIPOC) populations highlight the need for intersectional, structural interventions beyond individual resilience. Full article

This work explores the qualitative dynamics of the modified unstable time-fractional nonlinear Schrödinger equation (mUNLSE), a model applicable to nonlinear wave propagation in plasma and optical fiber media. By transforming the governing equation into a planar conservative Hamiltonian system, a detailed bifurcation study is carried out, and the associated equilibrium points are classified using Lagrange’s theorem and phase-plane analysis. A family of exact wave solutions is then constructed in terms of both trigonometric and Jacobi elliptic functions, with solitary, kink/anti-kink, periodic, and super-periodic profiles emerging under suitable parameter regimes and linked directly to the type of the phase plane orbits. The validity of the solutions is discussed through the degeneracy property which is equivalent to the transmission between the phase orbits. The influence of the fractional derivative order on amplitude, localization, and dispersion is illustrated through graphical simulations, exploring the memory impacts in the wave evolution. In addition, an externally periodic force is allowed to act on the mUNLSE model, which is reduced to a perturbed non-autonomous dynamical system. The response to periodic driving is examined, showing transitions from periodic motion to quasi-periodic and chaotic regimes, which are further confirmed by Lyapunov exponent calculations. These findings deepen the theoretical understanding of fractional Schrödinger-type models and offer new insight into complex nonlinear wave phenomena in plasma physics and optical fiber systems. Full article

Background: Gestational diabetes mellitus (GDM) is associated with metabolic disturbance and subclinical cardiovascular changes during pregnancy and after birth. Optimal glycaemic control remains challenging for many patients despite existing management strategies. Ursodeoxycholic acid (UDCA) has shown potential metabolic effects, including enhanced insulin sensitivity and anti-inflammatory effects. Previously, we demonstrated that UDCA improves glycaemic control in women achieving higher circulating UDCA concentrations; however, its effect on maternal cardiac function remains unknown. The objective was to evaluate whether treatment with UDCA compared with placebo is associated with differences in maternal cardiac function in pregnancies complicated by GDM. Methods: In this randomized, placebo-controlled trial, 113 women with GDM were recruited, with 56 allocated to UDCA and 57 to placebo (IMIB-GU-2019-02, registration date: 17 June 2020; first participant enrolled: 3 March 2021). After measurement of maternal blood UDCA levels, 43 participants in the treatment group with levels ≥ 0.5 μmol/L were included in a per-protocol analysis. Participants had cardiac assessments at baseline, in the late third trimester (36 weeks) and postpartum. Detailed left ventricular systolic and diastolic functional indices were assessed using conventional pulse and tissue Doppler indices as well as strain imaging. Right ventricular systolic function was also assessed. Results: Baseline maternal characteristics and cardiac functional indices were comparable between the UDCA and placebo groups. In the third trimester, women treated with UDCA showed more negative left atrial strain during atrial contraction (LASct_AC) compared with placebo (p = 0.016), while no significant between-group differences were observed in conventional left ventricular systolic or diastolic parameters. In the postpartum period, UDCA treatment was associated with higher left atrial reservoir function, reflected by increased LASr_ED (p = 0.041) and LASr_AC (p = 0.036), as well as more negative left atrial conduit strain at end-diastole (LAScd_ED; p = 0.043). No consistent differences were observed in left ventricular systolic function, haemodynamic indices, or right ventricular functional parameters between the two groups. Conclusions: These findings are associated with small and time-dependent differences in reducing atrial dysfunction and improving cardiac efficiency during late pregnancy and postpartum. However, given the lack of long-term follow-up, further research is needed to determine the long-term cardiovascular relevance of UDCA in this population. Full article

Low social status leads to chronic social stress that predicts risk for physical and mental illness, especially when it starts early in life. To examine the longitudinal effects of low social status on the immune system, this study assessed the effects of low social status on developmental secretory patterns of pro- and anti-inflammatory markers under baseline conditions, as well as in response to an immune challenge (lipopolysaccharide (LPS)-induced activation of pro- and anti-inflammatory cytokines) in a translational rhesus monkey model of lifelong social subordination stress. Baseline blood samples were collected in 27 socially housed female rhesus monkeys (13 dominants, DOM, and 14 subordinates, SUB) during infancy (6 months), the juvenile pre-pubertal period (16 months), and adulthood (9–10 years) to examine the longitudinal effects of social status on inflammatory markers in unstimulated versus LPS-stimulated conditions mimicking exposure to bacterial infection. Basal levels of the stress hormone cortisol in blood were measured to examine associations between inflammation and activity of the hypothalamic–pituitary–adrenal (HPA) axis throughout the life span. Basal peripheral levels of inflammatory markers (e.g., IL-6) increased across development in both SUB and DOM animals with no significant differences. Basal cortisol levels were significantly higher in infancy as compared to adulthood, but no significant effects of social rank were detected. However, in adulthood, SUB animals showed a cytokine-specific immune response to ex vivo LPS stimulation with significantly higher secretions of IL-1β, IL-2, and IL-10 compared to DOM animals, whereas IL-8 response to LPS was lower in SUB animals than in DOMs. This cytokine-specific response to an immune challenge that mimics bacterial infection could reflect dysregulated immune cells that may have short-term adaptation, but at the cost of longer-term risks for low-grade chronic inflammation and accelerated immune aging for socially subordinate female macaques. Full article

Women exhibit a higher prevalence of depression, anxiety, stress-related disorders, and autoimmune conditions compared to men, yet the biological mechanisms underlying this sex difference remain incompletely understood. Growing evidence identifies neuroinflammation as a central mediator of psychiatric vulnerability in women, shaped by interactions between sex hormones, immune activation, and neural circuit regulation. Throughout the female lifespan, fluctuations in estrogen and progesterone, such as those occurring during puberty, the menstrual cycle, pregnancy, postpartum, and perimenopause, modulate microglial activity, cytokine release, and neuroimmune signaling. These hormonal transitions create windows of heightened sensitivity in key brain regions involved in affect regulation, including the amygdala, hippocampus, and prefrontal cortex. Parallel variations in systemic inflammation, mitochondrial function, and hypothalamic–pituitary–adrenal (HPA) axis responsivity amplify stress reactivity and autonomic imbalance, contributing to increased risk for mood and anxiety disorders in women. Emerging data also highlight sex-specific interactions between the immune system and monoaminergic neurotransmission, gut–brain pathways, endothelial function, and neuroplasticity. This review synthesizes current neuroscientific evidence on the sex-dependent neuroinflammatory mechanisms that bridge hormonal dynamics, brain function, and psychiatric outcomes in women. We identify critical periods of vulnerability, summarize converging molecular pathways, and discuss novel therapeutic targets including anti-inflammatory strategies, estrogen-modulating treatments, lifestyle interventions, and biomarkers for personalized psychiatry. Understanding neuroinflammation as a sex-specific process offers a transformative perspective for improving diagnosis, prevention, and treatment of psychiatric disorders in women. Full article

Wireless Sensor Networks (WSNs) operate under strict energy constraints and are therefore highly vulnerable to radio interference, particularly jamming attacks that directly affect communication availability and network lifetime. Although jamming and anti-jamming mechanisms have been extensively studied, energy is frequently treated as a secondary metric, and analyses are often conducted in partial isolation from system assumptions, protocol behavior, and deployment context. This fragmentation limits the interpretability and comparability of reported results. This article presents a systematic literature review (SLR) covering the period from 2004 to 2024, with a specific focus on energy-aware jamming and mitigation strategies in IEEE 802.15.4-based WSNs. To ensure transparency and reproducibility, the literature selection and refinement process is formalized through a mathematical search-and-filtering model. From an initial corpus of 482 publications retrieved from Scopus, 62 peer-reviewed studies were selected and analyzed across multiple dimensions, including jamming modality, affected protocol layers, energy consumption patterns, evaluation assumptions, and deployment scenarios. The review reveals consistent energy trends among constant, random, and reactive jamming strategies, as well as significant variability in the energy overhead introduced by defensive mechanisms at the physical (PHY), Medium Access Control (MAC), and network layers. It further identifies persistent methodological challenges, such as heterogeneous energy metrics, incomplete characterization of jamming intensity, and the limited use of real-hardware testbeds. To address these gaps, the paper introduces an energy-centric taxonomy that explicitly accounts for attacker–defender energy asymmetry, cross-layer interactions, and recurring experimental assumptions, and proposes a minimal set of standardized energy-related performance metrics suitable for IEEE 802.15.4 environments. By synthesizing energy behaviors, trade-offs, and application-specific implications, this review provides a structured foundation for the design and evaluation of resilient, energy-proportional WSNs operating under availability-oriented adversarial interference. Full article

Background and Objectives: Hysteroscopy has become the “gold standard” in assessing uterine cavity abnormalities, and currently it can be performed in an “office setting”. Although office hysteroscopy has a better level of comfort than operative hysteroscopy, pain is a common concern. Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used for pre-procedure analgesia, but they may cause gastrointestinal side effects. Paracetamol offers to be a safer alternative, but its efficacy in this setting is limited. This study aimed to compare the efficacy and safety of oral paracetamol with oral ketoprofen for pain management during office hysteroscopy. Materials and Methods: Double-blind, parallel-group, randomized controlled trial conducted at a single hysteroscopy center in Jakarta, Indonesia, over a 2-year period. Sixty women undergoing office hysteroscopy were randomized (1:1) to receive paracetamol 1000 mg orally or ketoprofen 100 mg orally 1 h before the procedure. Results: All participants completed the trial and were included in the analysis. The median visual analog score (VAS) during the procedure was 2 (range 0–8) in the paracetamol group versus 3 (range 0–6) in the ketoprofen group (p = 0.266). Median cramping scores 30 min post-procedure in the paracetamol group were 0 (range 0–5) vs. 0 (range 0–4) in the ketoprofen group, respectively (p = 0.499). Side effects occurred in 3 participants (10%) in the ketoprofen group and none of the paracetamol group. Comfort scores were high in both groups (median 9/10). No vagal reflexes were observed. Conclusions: Oral 1000 mg paracetamol was as effective as oral 100 mg ketoprofen for pain management during and after office hysteroscopy, with fewer side effects. Paracetamol may be a safe and cost-effective alternative for pre-procedure analgesia in office hysteroscopy. Full article

Diminished ovarian reserve (DOR) is a major cause of female infertility with limited treatment options, and lifestyle interventions such as supervised, structured exercise therapy may support ovarian function. In this pilot study, we evaluated the effect of a supervised, physiotherapy-based exercise program combined with antioxidant supplementation on ovarian reserve markers and spontaneous pregnancy rates in 24 infertile women aged 20–42 years, with body mass index (BMI) 18.5–30 kg/m², regular menstruation, anti-Müllerian hormone (AMH) < 1.1 ng/mL, and antral follicle count ≥3 measured on days 2–4 of the cycle. Participants were randomized into two groups of 12: Both groups received standardized oral therapy, while the intervention group additionally participated in a three-month supervised, structured exercise therapy programme. Analysis of covariance was used to adjust for baseline differences in AMH and BMI, as groups differed significantly in BMI at baseline. At post-treatment assessment, AMH levels were significantly higher in the intervention group, whereas FSH, LH, estradiol, prolactin, and TSH levels did not change significantly. Spontaneous pregnancies were recorded both during the intervention period and throughout a follow-up period of up to six months. Spontaneous pregnancy occurred in 7 out of 12 participants in the intervention group versus 1 out of 12 in the control group, resulting in four and one live births, respectively. These findings suggest that combining supervised, structured exercise therapy with antioxidant supplementation may enhance ovarian reserve and improve the likelihood of spontaneous pregnancy in women with diminished ovarian reserve. Full article