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14 pages, 2875 KB  
Article
Computational Identification of Genetic Background of Infertility and Calculating Inbreeding Coefficient in Dromedary Camel Herds
by Fahad A. Alshanbari and Abdulrahman Aloraini
Genes 2025, 16(10), 1238; https://doi.org/10.3390/genes16101238 (registering DOI) - 19 Oct 2025
Abstract
Background: Inbreeding is a major genetic problem that reduces fertility and causes genetic disorders. Some breeders of dromedary camels use the same bull for many years due to its excellent characteristics, leading to mating with offspring and subsequent generations, resulting in increased homozygosity [...] Read more.
Background: Inbreeding is a major genetic problem that reduces fertility and causes genetic disorders. Some breeders of dromedary camels use the same bull for many years due to its excellent characteristics, leading to mating with offspring and subsequent generations, resulting in increased homozygosity and genetic disorders. We hypothesize that inbreeding is associated with infertility in dromedary camels with normal and uninfected reproductive tracts. Methods: We genotyped 96 samples from seven camel breeds using the Illumina 55K SNP BeadChip, including five confirmed infertile individuals. Inbreeding coefficients (F) were calculated using PLINK based on heterozygosity and runs of homozygosity. Genome-wide association analysis using logistic regression was performed to identify potential genomic regions associated with infertility. Results: All five infertile camels showed significantly higher F values (>0.15) compared to 91 fertile individuals (<0.10, p < 0.001). The genome-wide association analysis failed to identify specific genomic regions linked to infertility, likely due to limited statistical power (n = 5 cases) and the polygenic nature of fertility traits. Population structure analysis revealed genetic differentiation related to coat color, with two significant SNPs on chromosome 3 near SLC30A5 (p < 107). Conclusions: Our results demonstrate that elevated inbreeding is strongly associated with infertility in dromedary camels. Future studies should employ larger sample sizes (≥50 infertile individuals) or whole-genome sequencing (35× coverage) to identify specific genomic regions. Implementation of breeding strategies avoiding related matings (F < 0.10) is recommended to maintain reproductive performance in camel herds. Full article
(This article belongs to the Section Animal Genetics and Genomics)
26 pages, 12747 KB  
Article
The Response of Alpine Permafrost to Decadal Human Disturbance in the Context of Climate Warming
by Shuping Zhang, Ji Chen, Lijun Huo, Xinyang Li, Chengying Wu, Hucai Zhang and Qi Feng
Remote Sens. 2025, 17(20), 3482; https://doi.org/10.3390/rs17203482 (registering DOI) - 19 Oct 2025
Abstract
Alpine permafrost plays a vital role in regional hydrology and ecology. Alpine permafrost is highly sensitive to climate change and human disturbance. The Muri area, which is located in the headwaters of the Datong River, northeast of the Tibetan Plateau, has undergone decadal [...] Read more.
Alpine permafrost plays a vital role in regional hydrology and ecology. Alpine permafrost is highly sensitive to climate change and human disturbance. The Muri area, which is located in the headwaters of the Datong River, northeast of the Tibetan Plateau, has undergone decadal mining, and the permafrost stability there has attracted substantial concerns. In order to decipher how and to what extent the permafrost in the Muri area has responded to the decadal mining in the context of climate change, daily MODIS land surface temperatures (LSTs) acquired during 2000–2024 were downscaled to 30 m × 30 m. The active layer thickness (ALT)–ground thaw index (DDT) coefficient was derived from in situ ALT measurements. An annual ALT of 30 m × 30 m spatial resolution was subsequently estimated from the downscaled LST for the Muri area using the Stefan equation. Validation of the LST and ALT showed that the root of mean squared error (RMSE) and the mean absolute error (MAE) of the downscaled LST were 3.64 °C and −0.1 °C, respectively. The RMSE and MAE of the ALT estimated in this study were 0.5 m and −0.25 m, respectively. Spatiotemporal analysis of the downscaled LST and ALT found that (1) during 2000–2024, the downscaled LST and estimated ALT delineated the spatial extent and time of human disturbance to permafrost in the Muri area; (2) human disturbance (i.e., mining and replantation) caused ALT increase without significant spatial expansion; and (3) the semi-arid climate, rough terrain, thin root zone and gappy vertical structure beneath were the major controlling factors of ALT variations. ALT, estimated in this study with a high resolution and accuracy, filled the data gaps of this kind for the Muri area. The ALT variations depicted in this study provide references for understanding alpine permafrost evolution in other areas that have been subject to human disturbance and climate change. Full article
19 pages, 20423 KB  
Article
Earthquake-Triggered Tsunami Hazard Assessment in the Santorini–Amorgos Tectonic Zone: Insights from Deterministic Scenario Modeling
by Dimitrios-Vasileios Batzakis, Dimitris Sakellariou, Efthimios Karymbalis, Loukas-Moysis Misthos, Gerasimos Voulgaris, Konstantinos Tsanakas, Emmanuel Vassilakis and Kalliopi Sapountzaki
J. Mar. Sci. Eng. 2025, 13(10), 2005; https://doi.org/10.3390/jmse13102005 (registering DOI) - 19 Oct 2025
Abstract
In the early months of 2025, a significant seismic activity was recorded in the area between Santorini and Amorgos, raising concerns about the potential occurrence of a major earthquake and a possible tsunami. The objective of this study is to assess the earthquake-triggered [...] Read more.
In the early months of 2025, a significant seismic activity was recorded in the area between Santorini and Amorgos, raising concerns about the potential occurrence of a major earthquake and a possible tsunami. The objective of this study is to assess the earthquake-triggered tsunami hazard in the Santorini-Amorgos Tectonic Zone (SATZ) by simulating tsunami processes using the MOST (Method of Splitting Tsunami) numerical model, implemented through the ComMIT (Community Model Interface for Tsunamis). High-resolution bathymetry and topography were employed to model tsunami generation, propagation, and onshore inundation. A total of 60 simulations were conducted using a deterministic approach based on worst-case scenarios. The analysis considered six major active faults with two kinematic types, pure normal and oblique-slip, and assessed tsunami impact on five selected coastal study areas. The simulations results showed potential maximum run-up values of 4.1 m in Gialos (Ios), 2.7 m in Kamari (Santorini), 2.4 m in Perissa (Santorini), 1.5 m in Katapola (Amorgos), and 2.3 m in Chora (Astypalaea), in some cases affecting residential zones. Inundation flows also impacted the main ports of Gialos, Katapola, and Chora, highlighting the exposure of critical infrastructure. Although earthquake-triggered tsunamis represent a potential hazard in the SATZ, the results indicated that it is unlikely to cause a widespread disaster in the study areas. Full article
(This article belongs to the Special Issue Storm Tide and Wave Simulations and Assessment, 3rd Edition)
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22 pages, 6783 KB  
Article
Parsing Glomerular and Tubular Structure Variability in High-Throughput Kidney Organoid Culture
by Kristiina Uusi-Rauva, Anniina Pirttiniemi, Antti Hassinen, Ras Trokovic, Sanna Lehtonen, Jukka Kallijärvi, Markku Lehto, Vineta Fellman and Per-Henrik Groop
Methods Protoc. 2025, 8(5), 125; https://doi.org/10.3390/mps8050125 (registering DOI) - 19 Oct 2025
Abstract
High variability in stem cell research is a well-known limiting phenomenon, with technical variation across experiments and laboratories often surpassing variation caused by genotypic effects of induced pluripotent stem cell (iPSC) lines. Evaluation of kidney organoid protocols and culture conditions across laboratories remains [...] Read more.
High variability in stem cell research is a well-known limiting phenomenon, with technical variation across experiments and laboratories often surpassing variation caused by genotypic effects of induced pluripotent stem cell (iPSC) lines. Evaluation of kidney organoid protocols and culture conditions across laboratories remains scarce in the literature. We used the original air-medium interface protocol to evaluate kidney organoid success rate and reproducibility with several human iPSC lines, including a novel patient-derived GRACILE syndrome iPSC line. Organoid morphology was assessed with light microscopy and immunofluorescence-stained maturing glomerular and tubular structures. The protocol was further adapted to four microplate-based high-throughput approaches utilizing spheroid culture steps. Quantitative high-content screening analysis of the nephrin-positive podocytes and ECAD-positive tubular cells revealed that the choice of approach and culture conditions were significantly associated with structure development. The culture approach, iPSC line, experimental replication, and initial cell number explained 35–77% of the variability in the logit-transformed proportion of nephrin and ECAD-positive area, when fitted into multiple linear models. Our study highlights the benefits of high-throughput culture and multivariate techniques to better distinguish sources of technical and biological variation in morphological analysis of organoids. Our microplate-based high-throughput approach is easily adaptable for other laboratories to combat organoid size variability. Full article
(This article belongs to the Section Omics and High Throughput)
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24 pages, 6262 KB  
Article
Selective Removal of Arsenic and Antimony by Alkaline Leaching with Sodium Sulfide: Remediation of Metalloids-Contaminated Concentrates from Zimapán, Hidalgo, Mexico
by Gabriel Cisneros, Julio C. Juárez, Iván A. Reyes, Martín Reyes, Gustavo Urbano, Jesús I. Martínez, Aislinn M. Teja and Mizraim U. Flores
Processes 2025, 13(10), 3347; https://doi.org/10.3390/pr13103347 (registering DOI) - 19 Oct 2025
Abstract
Selective alkaline leaching was evaluated to remove arsenic (As) and antimony (Sb) from a polymetallic copper concentrate from Zimapán, Mexico, where these metalloids cause environmental risk and smelter penalties. Batch tests used sodium sulfide (Na2S) in alkaline media, varying reagent concentrations [...] Read more.
Selective alkaline leaching was evaluated to remove arsenic (As) and antimony (Sb) from a polymetallic copper concentrate from Zimapán, Mexico, where these metalloids cause environmental risk and smelter penalties. Batch tests used sodium sulfide (Na2S) in alkaline media, varying reagent concentrations and temperature; kinetic modeling identified the rate-controlling step, and X-ray diffraction (XRD) plus scanning electron microscopy/energy-dispersive spectroscopy (SEM–EDS) assessed phase changes. The kinetic analysis indicated chemical control with a higher reaction order for Na2S than for NaOH. A quadratic regression described the process and identified Na2S concentration and temperature as the dominant factors. Maximum extractions reached 91.9% for As and 72.1% for Sb while limiting dissolution of value-bearing sulfides, as supported by XRD and SEM–EDS. Environmental indices (Igeo, EF) classified As and Sb as highly contaminating and geochemically enriched in the feed, underscoring the need for selective removal. Overall, alkaline leaching with Na2S provides a technically feasible and environmentally favorable route to remediate metalloids and upgrade polymetallic concentrates. Full article
16 pages, 3732 KB  
Article
Comprehensive Transcriptomic Analysis of the Molecular Mechanisms Conferring Resistance to Rice Blast in the Elite Restorer Line Fuhui2165
by Shuijin Zhang, Yinyin Mao, Yonghe Hong, Feiyan Zheng, Ronghua Hu, Shihang Tu, Fantao Zhang and Peng Zhou
Int. J. Mol. Sci. 2025, 26(20), 10164; https://doi.org/10.3390/ijms262010164 (registering DOI) - 19 Oct 2025
Abstract
Rice blast, caused by Magnaporthe oryzae (M. oryzae), severely threatens global rice production with substantial yield losses, endangering food security and driving demand for resistant varieties. Fuhui2165 (FH2165), an elite restorer line with stable blast resistance, superior agronomic traits, and high [...] Read more.
Rice blast, caused by Magnaporthe oryzae (M. oryzae), severely threatens global rice production with substantial yield losses, endangering food security and driving demand for resistant varieties. Fuhui2165 (FH2165), an elite restorer line with stable blast resistance, superior agronomic traits, and high grain quality, is valuable for hybrid breeding, but its resistance mechanisms remain unclear. In this study, we investigated the rice blast resistance and underlying mechanisms in FH2165 and its parental lines (Huahangsimiao/HHSM, Minghui86/MH86, and Shuhui527/SH527) using transcriptome sequencing analysis. Phenotypic analysis revealed that FH2165 and HHSM exhibited stronger resistance compared to MH86 and SH527. Differential expression analysis identified 3886, 2513, 3390, and 4678 differentially expressed genes (DEGs) in FH2165, HHSM, MH86, and SH527, respectively. Gene Ontology (GO) enrichment analysis highlighted DEGs associated with chloroplasts, plastids, thylakoids, and related cellular components. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis identified significant enrichment in pathways such as carbon metabolism, amino acid biosynthesis, and photosynthesis. This suggested that defense strategies could involve energy reprogramming and the synthesis of secondary metabolites. Additionally, the DEGs co-expressed specifically in FH2165 and HHSM were enriched in functions related to RNA processing, GTP binding, and L-ascorbic acid binding, with purine metabolism playing a role in the regulation of energy and signaling. These findings elucidated the critical metabolic and signaling networks that underlie the blast resistance of FH2165 and offered potential targets for breeding high-yield, disease-resistant hybrid rice varieties. Full article
(This article belongs to the Special Issue Plant Stress Biology)
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12 pages, 1963 KB  
Article
Morphometry and Morphology of the Body and External Genitalia of Triatoma dimidiata (Hemiptera: Reduviidae) Morphotypes
by Karla Y. Acosta-Viana, Carlos M. Baak-Baak, Julio C. Tzuc-Dzul, Isabel Y. Chel-Muñoz, José I. Chan-Pérez, Wilbert A. Chi-Chim, Julian E. Garcia-Rejon, Frida Álvarez-León, Irving May-Concha, Angélica Pech-May and Nohemi Cigarroa-Toledo
Taxonomy 2025, 5(4), 61; https://doi.org/10.3390/taxonomy5040061 (registering DOI) - 19 Oct 2025
Abstract
In Yucatán state, Mexico, Triatoma dimidiata (Latreille, 1811) is the primary vector of Trypanosoma cruzi, the parasite that causes Chagas disease. The vector population presents diverse forms and colorations. Therefore, this study was designed to determine the morphotypes of T. dimidiata based [...] Read more.
In Yucatán state, Mexico, Triatoma dimidiata (Latreille, 1811) is the primary vector of Trypanosoma cruzi, the parasite that causes Chagas disease. The vector population presents diverse forms and colorations. Therefore, this study was designed to determine the morphotypes of T. dimidiata based on the taxonomy of the body and external genitalia. Between March 2023 and April 2025, 902 triatomines from 15 municipalities were examined. Three main morphotypes were characterized (I to III). Morphotype II was the most abundant (62.86%) and most distributed in the study area (12 of 15 municipalities), with a notable presence in forests and caves. Morphotypes I and III were found primarily outside houses and in chicken coops. Within the characterized specimens of T. dimidiata sensu lato, morphotype II displays more prominent morphological and structural characteristics. They are smaller compared to morphotypes I and III. In morphotype II, the spiracles are covered by a black spot that extends from the connexival plate to the urosternites. Males had short and robust parameres. The median process of the pygophore is long and slender compared to morphotypes I and III. The female tergite VIII has six sides. The taxonomy should be complemented by a study of the life cycle of each morphotype and analysis of its genome. Full article
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23 pages, 17232 KB  
Article
From Mechanical Instability to Virtual Precision: Digital Twin Validation for Next-Generation MEMS-Based Eye-Tracking Systems
by Mateusz Pomianek, Marek Piszczek, Paweł Stawarz and Aleksandra Kucharczyk-Drab
Sensors 2025, 25(20), 6460; https://doi.org/10.3390/s25206460 (registering DOI) - 18 Oct 2025
Abstract
The development of high-performance MEMS-based eye trackers, crucial for next-generation medical diagnostics and human–computer interfaces, is often hampered by the mechanical instability and time-consuming recalibration of physical prototypes. To address this bottleneck, we present the development and rigorous validation of a high-fidelity digital [...] Read more.
The development of high-performance MEMS-based eye trackers, crucial for next-generation medical diagnostics and human–computer interfaces, is often hampered by the mechanical instability and time-consuming recalibration of physical prototypes. To address this bottleneck, we present the development and rigorous validation of a high-fidelity digital twin (DT) designed to accelerate the design–test–refine cycle. We conducted a comparative study of a physical MEMS scanning system and its corresponding digital twin using a USAF 1951 test target under both static and dynamic conditions. Our analysis reveals that the DT accurately replicates the physical system’s behavior, showing a geometric discrepancy of <30 µm and a matching feature shift (1 µm error) caused by tracking dynamics. Crucially, the DT effectively removes mechanical vibration artifacts, enabling the precise analysis of system parameters in a controlled virtual environment. The validated model was then used to develop a pupil detection algorithm that achieved an accuracy of 1.80 arc minutes, a result that surpasses the performance of a widely used commercial system in our comparative tests. This work establishes a validated methodology for using digital twins in the rapid prototyping and optimization of complex optical systems, paving the way for faster development of critical healthcare technologies. Full article
(This article belongs to the Section Sensors and Robotics)
22 pages, 2310 KB  
Article
Molecular Epidemiology of Hepatitis E Virus in Hungary (2018–2025): Emergence of Rare Subtypes and First Detection of HEV-4 in Central Europe
by Ágnes Dencs, Andrea Hettmann, Levente Zsichla, Viktor Müller, Anett Dömötör, Ágnes Barna-Lázár, Erzsébet Barcsay and Mária Takács
Viruses 2025, 17(10), 1389; https://doi.org/10.3390/v17101389 (registering DOI) - 18 Oct 2025
Abstract
Hepatitis E virus (HEV) is an emerging cause of viral hepatitis in Europe, with increasing recognition in immunocompromised patients. While genotype 3 (HEV-3) is most prevalent in the region, molecular epidemiology data from Hungary have been limited. HEV strains from 118 RNA-positive patients [...] Read more.
Hepatitis E virus (HEV) is an emerging cause of viral hepatitis in Europe, with increasing recognition in immunocompromised patients. While genotype 3 (HEV-3) is most prevalent in the region, molecular epidemiology data from Hungary have been limited. HEV strains from 118 RNA-positive patients diagnosed between 2018 and 2025 were genotyped. Next-generation sequencing yielded near-complete HEV genomes for 76 samples. HEV-3 was dominant (98.3%). Subtype 3a was the most common (34.7%), followed by 3c, 3f, and 3e. Rare subtypes (3g, 3h, 3i, 3m, 3ra) and HEV-4b were detected for the first time in Hungary. Among immunocompromised patients, 41.6% developed chronic infection. Ribavirin resistance-associated mutations G1634R and V1479I were frequently detected. In silico analysis of potential multiple infections indicated the presence of at least two HEV strains of distinct origin in six patients. Our surveillance revealed extensive genetic diversity of HEV in Hungary. The detection of rare HEV-3 subtypes and the first documented occurrence of HEV-4b in the country highlight likely viral introductions linked to the increasing international trade. Ongoing surveillance is essential in protecting high-risk groups and limiting HEV transmission in a globalized food system. Full article
(This article belongs to the Section Human Virology and Viral Diseases)
16 pages, 2135 KB  
Article
Biodegradable PVA–Alginate Bio-Based Polymers Incorporating Cardanol-Based Polyols for Antibacterial Applications
by Da Hae Lee, Hee Ju Ahn, Jaekyoung Lee and Hee Chul Woo
Polymers 2025, 17(20), 2792; https://doi.org/10.3390/polym17202792 (registering DOI) - 18 Oct 2025
Abstract
The extensive use of petroleum-based plastics has caused serious environmental concerns; thus, the need for biodegradable alternatives is essential. Here, we present eco-friendly bio-based polymers prepared by crosslinking poly(vinyl alcohol) (PVA) and alginate (ALG) with glutaraldehyde, while incorporating cardanol-derived polyols (PCD) to add [...] Read more.
The extensive use of petroleum-based plastics has caused serious environmental concerns; thus, the need for biodegradable alternatives is essential. Here, we present eco-friendly bio-based polymers prepared by crosslinking poly(vinyl alcohol) (PVA) and alginate (ALG) with glutaraldehyde, while incorporating cardanol-derived polyols (PCD) to add antibacterial functionality. The synthesized bio-based polymers were characterized by FT-IR, XRD, and TGA. FT-IR confirmed sufficient crosslinking between PVA and ALG, whereas XRD revealed a minor decrease in crystallinity. Thermogravimetric analysis showed enhanced thermal stability with increasing ALG contents, as the residual mass increased from 8 wt% (PVA only) to 19–31% (PVA:ALG = 80:20–60:40). Swelling behavior was strongly governed by ALG, with higher ratios promoting water uptake up to 130%, whereas PCD reduced swelling due to increased hydrophobicity. Antibacterial assays indicated complete inactivation of Escherichia coli and Staphylococcus aureus within 10–60 min depending on the polymer composition. These results demonstrate that tuning the PVA:ALG ratio and PCD content allows precise control of physicochemical properties. Overall, the developed PVA–ALG/PCD bio-based polymers represent a versatile and sustainable platform for eco-friendly packaging, biomedical, and water treatment applications. Full article
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10 pages, 457 KB  
Article
Prevalence and Risk Factors of Musculoskeletal Pain Among Kuwaiti Pilgrims During Hajj 2024
by Tahra Aleid, Nowall Al-Sayegh, Sultan E. Alsalahi and Abdulaziz Alhenaidi
Int. J. Environ. Res. Public Health 2025, 22(10), 1585; https://doi.org/10.3390/ijerph22101585 (registering DOI) - 18 Oct 2025
Abstract
Background: Musculoskeletal pain (MSP) is one of the leading causes of disability worldwide and is frequently reported during the Muslim Hajj Pilgrimage; however, its prevalence and associated risk factors among Kuwaiti pilgrims have not been studied thus far. Methods: This is a retrospective [...] Read more.
Background: Musculoskeletal pain (MSP) is one of the leading causes of disability worldwide and is frequently reported during the Muslim Hajj Pilgrimage; however, its prevalence and associated risk factors among Kuwaiti pilgrims have not been studied thus far. Methods: This is a retrospective cross-sectional study of Kuwaiti pilgrims conducted during the year 2024. Pilgrims were contacted by phone before and after Hajj to answer a survey regarding MSP during their pilgrimage. Risk ratios were computed using binomial generalised linear models with a log link. Results: A total of 557 participants (Mean BMI 28.0 ± 8.0 kg/m2), comprising 340 women (61%) and 217 men (39%), participated in the study. Most were between 33 and 45 years of age (n = 173, 31%), with 24% of the sample (n = 136) reporting MSP. Our regression analysis revealed that female gender (aRR 1.49, 95% CI 1.08–2.06), short sleep duration (<6 h; aRR 1.37, 95% CI 1.02–1.84), and smoking (aRR 0.66, 95% CI 0.46–0.95) were significantly associated with MSP, while participants who did not report hypertension were also less likely to report MSP (aRR 0.64, 95% CI 0.46–0.89). Conclusions: This study, the first to focus on Kuwaiti pilgrims in this regard, showed that their reported prevalence of MSP during Hajj was lower than reported previously in studies of other nationalities. Several factors that increased the risk of MSP included smoking, hypertension, poor sleep, and female gender. The results of this study emphasise the necessity of both conducting a screening programme before Hajj and raising awareness of the factors that increase the prevalence of MSP, subsequently reducing the readiness of pilgrims. Full article
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20 pages, 1181 KB  
Article
The Potential Public Health Impact of the mRNA-Based Respiratory Syncytial Virus Vaccine, mRNA-1345, Under Extended Vaccination Campaigns Among Older Adults in the United Kingdom: A Modelling Study
by Mariia Dronova, Anna Tytuła, Zuzanna Janusz, Parinaz Ghaswalla, Stuart Carroll, Orsolya Balogh and Keya Joshi
Vaccines 2025, 13(10), 1065; https://doi.org/10.3390/vaccines13101065 (registering DOI) - 18 Oct 2025
Abstract
Background/Objectives: Respiratory syncytial virus (RSV) is a leading cause of severe respiratory disease in older adults. Despite growing recognition of RSV as a public health concern, vaccination options remain limited. This study assessed the potential long-term public health impact of extended mRNA-1345 [...] Read more.
Background/Objectives: Respiratory syncytial virus (RSV) is a leading cause of severe respiratory disease in older adults. Despite growing recognition of RSV as a public health concern, vaccination options remain limited. This study assessed the potential long-term public health impact of extended mRNA-1345 RSV vaccination campaigns. Methods: A dynamic transmission model, stratified by age, was developed to evaluate the epidemiological and clinical impact of RSV vaccination in the UK over a 20-year time horizon. Eight vaccination strategies were assessed: two reflecting the JCVI recommendation for the 2024–2025 season and its recent extension, and six extended strategies considering broader eligible age groups, higher coverage, and/or revaccination every 2 or 3 years. Two exploratory analyses and extensive model validation versus reported data were also conducted. Results: Strategies combining broader age eligibility (≥60 years), higher coverage (80%), and 2-year revaccination achieved the greatest impact, preventing 310,000 hospitalisations over 20 years in the total UK population. Exploratory analyses showed that the expected public health impact might exceed the estimates presented in this analysis, if an alternative vaccine efficacy profile or the projected demographic shift would be confirmed. Conclusions: Extended RSV vaccination strategies including broader age eligibility and routine revaccination could offer substantial public health benefits in the UK. Targeting adults aged ≥60 years is expected to be particularly efficient in achieving a sustainable reduction in RSV burden. These findings could provide valuable support for national policy discussions on optimising RSV vaccination strategies in older adults, particularly regarding target age groups, revaccination schedules, and long-term programme planning. Full article
(This article belongs to the Special Issue Respiratory Syncytial Virus (RSV) Vaccine)
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37 pages, 6657 KB  
Article
A Hybrid in Silico Approach for Identifying Dual VEGFR/RAS Inhibitors as Potential Anticancer and Anti-Angiogenic Agents
by Alessia Bono, Gabriele La Monica, Federica Alamia, Dennis Tocco, Antonino Lauria and Annamaria Martorana
Pharmaceuticals 2025, 18(10), 1579; https://doi.org/10.3390/ph18101579 (registering DOI) - 18 Oct 2025
Abstract
Background: Angiogenesis, the physiological process by which new blood vessels originate from pre-existing ones, can be triggered by tumor cells to promote the growth, survival, and progression of cancer. Malignant tumors require a constant blood supply to meet their needs for oxygen [...] Read more.
Background: Angiogenesis, the physiological process by which new blood vessels originate from pre-existing ones, can be triggered by tumor cells to promote the growth, survival, and progression of cancer. Malignant tumors require a constant blood supply to meet their needs for oxygen and nutrients, making angiogenesis a key process in tumor development. Its pathologic role is caused by the dysregulation of signaling pathways, particularly those involving VEGFR-2, a key mediator of angiogenesis, and the K-RAS G12C mutant, a promoter of VEGF expression. Given their critical involvement in tumor progression, these targets represent promising candidates for new cancer therapies. Methods and Results: In this study, we applied an in silico hybrid and hierarchical virtual screening approach to identify potential dual VEGFR-2/K-RAS G12C inhibitors with anticancer and antiangiogenic properties. To this end, we screened the National Cancer Institute (NCI) database through ADME filtering tools. The refined dataset was then submitted to the ligand-based Biotarget Predictor Tool (BPT) in a multitarget mode. Subsequently, structure-based analysis, including molecular docking studies on VEGFR and K-RAS G12C, was performed to investigate the interactions of the most promising small molecules with both targets. Conclusions: Finally, the molecular dynamics simulations suggested compound 737734 as a promising small molecule with high stability in complex with both VEGFR-2 and K-RAS G12C, highlighting its potential as a dual-target inhibitor for cancer therapy. Full article
(This article belongs to the Special Issue Application of Computer Simulation in Drug Design)
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14 pages, 9534 KB  
Article
Failure Analysis of Gear on Rail Transit
by An-Xia Pan, Chao Wen, Haoyu Wang, Ping Tao, Xuedong Liu, Yi Gong and Zhen-Guo Yang
Materials 2025, 18(20), 4773; https://doi.org/10.3390/ma18204773 (registering DOI) - 18 Oct 2025
Abstract
The gear transmission system is a safety-critical component in rail transit, typically designed for a service life exceeding 20 years. Failure analysis of such systems remains a key focus for railway engineers. This study systematically investigates four representative cases of premature gear failure [...] Read more.
The gear transmission system is a safety-critical component in rail transit, typically designed for a service life exceeding 20 years. Failure analysis of such systems remains a key focus for railway engineers. This study systematically investigates four representative cases of premature gear failure in high-speed trains using a standardized analytical procedure that includes visual inspection, chemical analysis, metallographic examination, scanning electron microscopy, and hardness testing. The results identify four primary root causes: subsurface slag inclusions in raw materials, inadequate heat treatment leading to a non-martensitic layer (∼60 μm) at the tooth root, grinding-induced temper burns (crescent-shaped "black spots") accompanied by a hardness drop of ∼100–150 HV, and insufficient lubrication. The interdependencies between these factors and failure mechanisms, e.g., fatigue cracking, spalling, and thermal scuffing, are analyzed. This work provides an evidence-based framework for improving gear reliability and proposes targeted countermeasures, such as ultrasonic inclusion screening and real-time grinding temperature control, to extend operational lifespans. Full article
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Article
Development of an Electrochemical Sensor Based on Molecularly Imprinted Polymer Using Functionalized Gold Nanoparticles for Caffeine Quantification
by Sergio Espinoza-Torres, Astrid Choquehuanca-Azaña, Marcos Rufino, Eleilton da Silva and Lucio Angnes
Biosensors 2025, 15(10), 704; https://doi.org/10.3390/bios15100704 (registering DOI) - 18 Oct 2025
Abstract
Caffeine is a natural alkaloid consumed primarily for its stimulant and metabolic effects. Some everyday products, such as coffee, tea, soft drinks, sports supplements, and even pain relievers, contain caffeine. However, excessive caffeine consumption, greater than 400 mg per day, can cause adverse [...] Read more.
Caffeine is a natural alkaloid consumed primarily for its stimulant and metabolic effects. Some everyday products, such as coffee, tea, soft drinks, sports supplements, and even pain relievers, contain caffeine. However, excessive caffeine consumption, greater than 400 mg per day, can cause adverse effects. Therefore, this work presents an electrochemical sensor based on a molecularly imprinted polymer (MIP) electropolymerized on gold nanoparticles functionalized with p-aminothiophenol (AuNPs-pATP) for caffeine quantification. AuNPs-pATP synthesized show a spherical morphology with an average diameter of 2.54 nm. Stages of MIP formation were monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) using a potassium ferrocyanide redox probe, where the following were observed: (i) an increase in conductivity upon modification of the GCE with AuNPs-pATP, (ii) the blocking of active sites during the electropolymerization step, and (iii) the release of specific cavities upon template removal, revealing consistent differences between the MIP and the control polymer (NIP). SEM images revealed three-dimensional spherical cavities on MIP surface, while the NIP showed a more compact rough surface. Caffeine quantification was performed using square wave voltammetry (SWV) with LOD of 0.195 µmol L−1 and LOQ of 0.592 µmol L−1. Interference studies indicated high selectivity and a high density of caffeine-specific binding sites in the MIP. Additionally, MIP sensor demonstrated reusability, good reproducibility, and stability, as well as promising results for analysis in soft drink and sports supplement samples. Full article
(This article belongs to the Special Issue Recent Advances in Molecularly Imprinted-Polymer-Based Biosensors)
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