Search Results (14,841)

Background/Objectives: Wide Awake Local Anesthesia No Tourniquet (WALANT) and ultrasound-guided peripheral nerve blocks (PNBs) are increasingly used alternatives to general anesthesia for hand and forearm surgery. While WALANT is commonly perceived as a time-efficient and resource-sparing technique, comparative data regarding patient satisfaction, perioperative pain, and time-related outcomes remain inconsistent. This study aimed to compare WALANT and ultrasound-guided supraclavicular peripheral nerve block techniques with respect to patient satisfaction, perioperative pain, time-related parameters, and surgeon-related outcomes in elective hand and forearm extremity surgery. Methods: This prospective comparative observational study included 80 adult patients undergoing elective hand or forearm surgery. Patients received either WALANT or ultrasound-guided supraclavicular brachial plexus block according to patient preference. The primary outcome was overall patient satisfaction assessed within 24 h postoperatively. Secondary outcomes included block performance time, waiting time, total anesthesia-related time, intraoperative and postoperative pain scores, additional sedation requirements, postoperative numbness, willingness to choose the same anesthetic technique again, safety outcomes and surgeon satisfaction. Results: Overall patient satisfaction was significantly higher in the peripheral nerve block group compared with the WALANT group (median [IQR]: 90 [85–100] vs. 80 [70–90], p < 0.0001). Intraoperative and postoperative pain scores were also significantly lower in the peripheral nerve block group. Although block performance time was longer with the peripheral nerve block, waiting time and total anesthesia-related time were significantly shorter compared with WALANT. Surgeon satisfaction and the need for additional intraoperative sedation did not differ significantly between groups. Conclusions: In elective hand and forearm surgery, ultrasound-guided supraclavicular peripheral nerve block was associated with higher patient satisfaction, lower pain scores, and shorter total anesthesia-related time compared with WALANT. Surgical satisfaction scores were similar with both anesthetic techniques. Considering the heterogeneity of clinical settings and procedural requirements, as well as cost and resource utilization considerations, anesthetic technique selection should be individualized. Full article

Coffee cherry pulp is an abundant by-product of coffee processing and contains bioactive compounds with potential value in animal nutrition. This study evaluated fermented coffee cherry pulp (CCF) as a feed additive in Thai native chickens. Five hundred day-old chicks were randomly allocated to five treatments: basal diet (CON), antibiotic growth promoter (AGP), and basal diets supplemented with 0.5, 1.0, or 2.0 g/kg CCF for 12 weeks. Supplementation with 1.0 g/kg CCF significantly increased final body weight and average daily gain and significantly improved feed conversion ratio compared with the basal diet (p < 0.05). Serum triglyceride levels were significantly reduced in the CCF1.0 and CCF2.0 groups (p < 0.05), while liver and kidney function indicators were not affected. Cooking loss was significantly lower in CCF-supplemented groups, and thigh muscle crude protein content was significantly higher at 0.5 and 1.0 g/kg (p < 0.05). Alpha diversity of cecal microbiota was not significantly altered, but directional shifts in community composition were observed, including enrichment of short-chain fatty acid-associated genera. Functional prediction indicated differences in carbohydrate and amino acid metabolic pathways. These results support fermented coffee cherry pulp as a potential alternative to antibiotic growth promoters in native chicken production. Full article

Background/Objectives: The increasing prevalence of antimicrobial-resistant bacteria highlights the need for improved methodologies to evaluate antimicrobial activity beyond conventional minimum inhibitory concentration testing. While resazurin-based assays are widely used for minimum inhibitory concentration determination due to their simplicity and sensitivity, minimum bactericidal concentration assessment still relies on labor-intensive colony-forming unit counting. The objective of this study was to develop and validate a resazurin-based microwell assay capable of determining both the minimum inhibitory concentration and the minimum bactericidal concentration without routine plate counting, thereby simplifying bactericidal evaluation. Methods: A two-step resazurin-based fluorescence assay was designed and performed in microplates. After determining the minimum inhibitory concentration using resazurin as a metabolic indicator, well-showing inhibited bacterial growths were subjected to a regrowth phase by transferring aliquots into fresh antimicrobial-free medium containing resazurin. This additional step allowed discrimination between reversible metabolic inhibition and irreversible bacterial death. The method was evaluated using ciprofloxacin and chloramphenicol against four bacterial species: Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa. Minimum bactericidal concentration values obtained using this assay were compared with those obtained through conventional colony counting on agar plates. Results: Minimum bactericidal concentration values obtained using the two-step fluorescence assay were fully concordant with the conventional colony-forming unit counting method for all tested antibiotics and bacterial species. Conclusions: The proposed two-step resazurin-based microwell assay represents a rapid, reliable, and less labor-intensive alternative for the determination of both the minimum inhibitory concentration and the minimum bactericidal concentration, with potential applications in clinical and industrial microbiology laboratories. Full article

Algae extracts have emerged as a sustainable and eco-friendly alternative to synthetic dyes and functional additives in the textile industry, particularly for dyeing and functionalizing of cotton fabrics. Herein, two types of water-soluble algae extracts from Arthrospira platensis and Porphyridium cruentum were characterized in terms of thermal, structural, and functional properties and used as dye and/or functional agents. Cotton samples were pre-treated with chitosan and alum mordants and compared with commercially treated cationic cotton. The algae extracts were applied through the exhaust method, with variations in temperature, pH, liquor ratio, temperature rise gradient, and extract percentages. The resulting colours, assessed through CIE L*a*b* coordinates and K/S values using UV–Vis spectroscopy, displayed green and pink coloration, with commercial cationic cotton exhibiting more intense colours. Colour fastness measurements were also performed on functionalized cotton fabrics. The water-based algae extracts and functionalized samples were additionally characterized for functional features, displaying an antioxidant activity exceeding 60% (68.13 ± 3.60 and 60.76 ± 1.18, for A. platensis and P. cruentum, respectively). This work highlights their dual role in providing both aesthetic dyeing and functional enhancement of cotton. By using renewable marine resources and eco-friendly water-based processes, this approach supports the development of greener, more sustainable textile technologies. Full article

In response to the alarming rise in antimicrobial resistance, bacteriophages have re-emerged as a promising alternative to conventional antibiotic therapy. The main objective of this paper was to characterize a newly isolated phage (vB_SEC_3) in the context of its suitability for phage therapy against MDR E. coli, which is considered a priority pathogen. The phage was characterized at the morphological, genomic, and biological levels relevant to phage therapy. TEM analyses revealed a non-enveloped icosahedral capsid lacking tail structure. Phylogenetic and tANI analyses placed the phage within the α3 phages (genus Alphatrevirus) of the less-studied family Microviridae and revealed <95% similarity to its closest relatives, suggesting vB_SEC_3 is a putative novel species within this genus. The genome (6085 bp, GC content 45.3%) displayed the conserved organization typical for these phages, including overlapping genes. No known genes associated with lysogeny, antibiotic resistance, or virulence were detected. Briefly, vB_SEC_3 was able to effectively lyse two MDR strains of E. coli (S1 and B5, EOP 0.735 and 0.961, respectively). Tolerance to a wide pH range (4–10.5) and to temperatures up to 80 °C was established. Six-month storage of the crude lysate at 4 °C resulted in a slight decrease (<0.16 log₁₀ PFU/mL) in phage titer. This study provides additional insights into the biology and diversity of Microviridae phages and offers a basis for future investigations into their potential relevance in the context of combating MDR bacteria. Full article

Osteosarcoma is a highly aggressive canine bone tumor characterized by early metastasis and resistance to chemotherapy. Vasculogenic mimicry (VM), the ability of tumor cells to form microvascular channels independent of endothelial cells, can contribute to tumor progression and poor prognosis. In this in vitro study, we evaluated the effects of celecoxib, a selective cyclooxygenase-2 (COX-2) inhibitor, on the canine osteosarcoma cell line D17. Celecoxib treatment significantly inhibited cell proliferation in a dose- and time-dependent manner, induced S-phase cell cycle arrest, and promoted apoptosis. Furthermore, celecoxib effectively disrupted VM formation on Matrigel. Transcriptome analysis revealed that celecoxib downregulated genes associated with angiogenesis and the COX pathway, notably PTGS2. Consistent with this, celecoxib treatment reduced the secretion of prostaglandin E2 (PGE2) in a dose-dependent manner. Crucially, the addition of exogenous PGE2 restored VM formation in celecoxib-treated cells, confirming that celecoxib-mediated VM suppression is dependent on the reduction of PGE2 levels. These findings establish the COX-2/PGE2 signaling axis as a key regulator of VM in D17 canine osteosarcoma cells and that celecoxib warrants further preclinical evaluation as a strategy to target both tumor growth and alternative vascularization. Full article

Neutrophil extracellular traps (NETs) critically influence thrombosis by promoting platelet aggregation, fibrin formation, and thrombus stabilisation. However, primary human neutrophils present experimental limitations, including short lifespan ex vivo and ethical concerns. In this article, we discuss the available data on PLB-985 cells, a neutrophil-like model with potential to replace human neutrophils in research. Additionally, we present novel structural comparisons showing that both PLB-985- and human neutrophil-derived NETs significantly increased fibrin fibre thickness compared to thrombin-only controls, with similar fibre morphology across conditions. Notably, we also see spherical particles resembling microvesicles within PLB-985-derived NETs, suggesting potential additional procoagulant effects via microvesicle-associated tissue factor level in these cells. New and existing data presented in this article suggest that differentiated PLB-985 cells are able to effectively replicate key structural and functional aspects of human neutrophil NETs. These observations support the use of PLB-985 cells as an ethical, reproducible, and practical alternative for in vitro studies of NETs. Further characterisation is required to determine differences between human neutrophils and neutrophil-like models in macrovesicle formation and implication in NET-related thrombosis research. Full article

Non-Intrusive Load Monitoring (NILM) enables appliance-level classification from aggregate electrical measurements and supports efficient energy management in smart buildings. However, the accuracy of existing NILM methods is often limited by the inability of conventional feature extraction techniques to capture nonlinear steady-state behavior. This study proposes a novel feature extraction framework for appliance classification, which integrates phase-space reconstruction (PSR) with 2-D Fourier series to derive geometry-based descriptors of appliance current waveforms. Unlike traditional signal-processing methods, the proposed approach utilizes the nonlinear geometric structure revealed by PSR and encodes it through Fourier descriptors, offering a discriminative, low-dimensional feature space suitable for classification using supervised machine learning algorithms. The method is evaluated on the high-resolution controlled single-appliance recordings from the COOLL dataset using the K-Nearest Neighbor (KNN) classifier. Extension to aggregated multi-appliance NILM scenarios would require additional stages such as event detection and load separation. Sensitivity analysis demonstrates that classification performance depends strongly on the choice of time delay and harmonic order, with optimal settings yielding an accuracy of up to 99.52% using KNN. The results confirm that larger time delays and a small number of harmonics effectively capture appliance-specific signatures. The findings highlight the effectiveness of PSR–Fourier-based geometric features as a robust alternative to conventional NILM feature extraction strategies. Full article

In modern production, Wire Arc Additive Manufacturing (WAAM) is becoming an essential technology for manufacturing complex components. However, the complexity of planning such processes constrains their widespread use in production cycles. Using various numerical simulation approaches allows for the investigation of resulting geometries with respect to process parameters, reducing the need for experiment-based process planning. Similar to various subtractive processes, there is increased interest in integrating simulation approaches into digital twin applications for planning and optimization of WAAM processes. This requires dynamic geometry mapping and simulation time comparable to the process duration. In this paper, a numerical simulation employing a Dexel-based geometry representation and a model for single-bead geometry parameter prediction is investigated as a vital alternative to Finite Element Method (FEM)-based simulations. The focus lies on the accuracy of the simulated components with respect to the simulation settings, the time needed for it to complete, and the degree of compliance between the simulated and produced multi-layer structures. Using optimized simulation settings achieves an accuracy loss of under 7% due to geometry discretization, with a simulation time that is approximately 37% faster than the process duration. The simulated components closely correspond to the experimental ones in terms of width and height, with a volumetric similarity ranging from 63.3% to 88.8%. Full article

Background: Given global population growth and aging, it is imperative to prioritize early eye disease detection and treatment. However, as patient volume increases, providers are facing a shortage of workforce capacity, particularly in areas where eye doctors are already scarce, making it important to consider alternative innovative solutions that could help increase eye screening capabilities. This study compared virtual reality (VR) platform of vision screening exams that are used to evaluate ocular health, such as 24-2 perimetry, Ishihara tiles, and the Amsler grid, against their in-clinic counterparts. Methods: A total of 86 subjects were recruited from Mount Sinai’s ophthalmology clinic (New York, USA) for a comparison trial that was internally controlled across healthy eyes and those with glaucoma and retinal diseases. VR and in-office tests were administered to the patients during their clinical visit, including 24-2 perimetry, Ishihara tiles, and the Amsler grid in a randomized order, and the results were compared for each test. Results: Perimetry results from Humphrey Visual Field Analyzer (HVFA) and VR suprathreshold testing demonstrated a good sensitivity both overall (80% OD, 84% OS) and across control (86% OD, 89% OS), glaucoma (69% OD, 78% OS), and retinal disease (76% OD, 80% OS) groups. A Garway-Heath anatomical map showed an overall 70–80% agreement. Ishihara plate tests did not show a significant difference between the two testing modalities (p = 0.12; Mann–Whitney U test), which remained true across all groups. Amsler grid testing differences were also non-significant within each subgroup (p = 0.81; Mann–Whitney U test). Patient time required to complete VR exams was significantly improved (p < 0.0001; Welch’s t-test) compared to the clinical standard tests. Conclusions: All VR-based exams tested in this study showed high sensitivity and percent agreement when compared to their in-office standards. Given the results of this study, VR has a promising potential in visual function screening, which, in addition to its portable design and easy use, could assist eye doctors in screening for prevalent diseases such as glaucoma and retinal conditions. Translational Relevance: VR-based vision exams that test vision fields, color vision and visual distortions provide comparable results in healthy patients, as well as those with glaucoma and retinal diseases, indicating its potential as a screening technology for different ocular pathologies. Given VR’s portable and low-profile features, it is important to consider leveraging VR to augment delivery of vision care. Full article

Hydroxyapatite (HAp) is a key bioceramic for biomedical applications, but conventional pressureless sintering (PS) requires high temperatures that can promote phase degradation. Here, we compare PS (1100 °C/180 min) and cold sintering process (CSP) (150 °C/450 MPa/30 min) for pure HAp and an HAp composite containing 4 wt.% niobium–phosphate bioglass (BG), using a 2 M H₃PO₄ transient liquid (10 wt.%). CSP increased relative density from 73.10% to 79.92% for HAp and from 68.43% to 83.54% for HAp/BG, representing up to a 22.1% gain compared with PS. One-way ANOVA confirmed a significant effect of processing route/composition on relative density (F(3,24) = 919.69, p < 0.05), and Tukey HSD indicated that all groups differed statistically. SEM revealed a markedly more consolidated and homogeneous microstructure for CSP, particularly for HAp/BG, consistent with enhanced dissolution–reprecipitation and pore filling. XRD showed that PS at 1100 °C led to partial HAp degradation with β-TCP formation, whereas CSP preserved the HAp phase with broader peaks, smaller crystallite size, and higher specific surface area. These results demonstrate CSP as an efficient low-temperature alternative for densifying HAp-based bioceramics, with BG addition further improving consolidation. Full article

The shipping sector is responsible for a considerable share of global CO₂ emissions and is under pressure to reduce emissions and adopt carbon-neutral fuels. Among the proposed alternatives, methanol produced from green hydrogen and biogenic CO₂ represents a promising option. However, the feasibility of its production is significantly influenced by the composition and variability of the bio-CO₂ feedstock, which can negatively impact the complete value chain. To address these challenges, sampling campaigns were carried out at actual bio-CO₂-emitting sites, namely biogas and biomass combustion facilities, to characterize the impurity profiles and determine the appropriate conditioning requirements. A novel membrane gas absorption system with a Diethanolamine solution was deployed directly in the field to capture, as well as purify to a certain extent, the CO₂ stream. The system demonstrated high efficiency in removing most impurities, achieving high CO₂ capture rates and impurity reduction close to 90%. However, residual chlorine species were detected in the CO₂ streams from biogas plants, suggesting the need for additional conditioning to meet the purity specifications required for methanol synthesis. Given that the feedstock composition and upstream process conditions could significantly affect the final output and present considerable variations, the implementation of additional cleaning measures is recommended before synthesis. Full article

In engineering practice, additively manufactured (AM) metal and metal alloy structural components, which often contain geometric discontinuities to fulfil functional requirements, are subjected to cyclic service loads. Among the possible loading configurations, far-field Mode I loading is frequently considered as a nominal reference condition. Within this context, a methodology for the fatigue assessment of notched AM Scalmalloy components subjected to Mode I far-field loading is proposed, combining the Strain Energy Density (SED) approach with a multiaxial critical plane-based fatigue criterion. The fatigue assessment is carried out at a verification point whose position is defined as a function of the characteristic length of the SED control volume for Mode I loading, determined through two alternative procedures, and of the surface roughness of the component. The proposed methodology is validated against experimental fatigue data available in the literature for AM Scalmalloy specimens featuring a circumferential semi-circular notch and subjected to Mode I far-field cyclic loading, which induces a locally multiaxial stress state at the notch root, given that the formulation does not rely on material-specific assumptions and could in principle be extended to other notched AM metal and metal alloy components. Full article

Dual-fuel combustion is often proposed for diesel engines as a means to partially replace conventional diesel with cleaner and/or more sustainable alternatives, such as those derived from green hydrogen. However, the low reactivity of these fuels (i.e., methane, hydrogen, and ammonia) often leads to prolonged ignition delay (ID) and combustion instability. This challenge could potentially be overcome using nanomaterials, which are additives that could improve reactivity and compensate for autoignition deficiencies. Thus, this study evaluates the effect of carbon nanotubes (CNTs) dispersed in diesel fuel on the autoignition process under dual-fuel operation. CNTs were dispersed at a concentration of 100 mg/L and stabilized with surfactant sodium dodecylbenzene sulfonate (SDBS). The resulting nanofuels were then tested in a constant volume combustion chamber (CVCC) using methane, hydrogen, and ammonia as secondary fuels across various energy substitution ratios and temperatures (535 °C, 590 °C and 650 °C). The results show that the impact of CNTs on ID is negligible, especially at high temperatures. At the lowest tested temperature (535 °C) and 40% methane substitution ratio, only slight reductions in ID were obtained. Nevertheless, this effect is less significant at higher temperatures (590 °C and 650 °C). Regarding pressure gradient, the addition of CNTs and SDBS generally induced a decrease in pressure-peak of up to 15%. This trend is attributed to the trapping of fuel droplets within the CNT structures, which creates a physical barrier that delays vaporization. Results confirm that autoignition, which is expected to be the main phenomenon influenced by CNT addition, is not enhanced. Full article

Background: Attention deficit hyperactivity disorder (ADHD) is a neurodevelopmental condition with increasing prevalence of adult diagnoses in Denmark. ADHD is characterized by inattention, hyperactivity, and impulsiveness. Conventional treatment is primarily pharmacological. Many adults with ADHD face challenges in maintaining structure in daily life and have an increased risk of developing stress, anxiety, and depression. Winter bathing is gaining popularity and is associated with improvements in mood, sleep quality, and the ability to handle stress. Objectives: The aim of this study was to explore how winter bathing was experienced to affect the mental well-being of five adults diagnosed with ADHD. Methods: A qualitative research design was used, with participant observation and semi-structured interviews with five participants in April 2025. Additional data were obtained through interviews with a psychologist and an ADHD mentor. Empirical data were thematically analysed, followed by theoretical analysis. Results: Six themes were revealed: Mental calmness: peace of mind and relief from racing thoughts; Bodily awareness and connection to the body; Joy: sustained positivity; Nature: essential for motivation and general mind–body calmness; Coping with and managing everyday life better; and Winter bathing as a supplement or alternative to medication for anxiety, depression and ADHD. Conclusions: Five adults with ADHD experienced winter bathing as a meaningful and motivating non-pharmacological intervention that strengthened their mental well-being and quality of life. Winter bathing promoted general mind–body calmness, sustained joy, bodily connection, and mental coping, and was used as a supplement or alternative to medication. Nature emerged as a key motivational factor enhancing presence and well-being. Full article