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22 pages, 1699 KB  
Article
The Quantitative Classification, Ordination and Diversity Characteristics of Plant Communities in Southwestern Tibet
by Xingle Qu, Han Wang and Daqing Luo
Diversity 2026, 18(6), 343; https://doi.org/10.3390/d18060343 - 5 Jun 2026
Viewed by 627
Abstract
To explore the distribution patterns of plant communities in southwestern Xizang and their relationships to environmental factors, this study focused on providing a theoretical basis for the conservation of biodiversity and ecological restoration of plant communities in the study area. Based on survey [...] Read more.
To explore the distribution patterns of plant communities in southwestern Xizang and their relationships to environmental factors, this study focused on providing a theoretical basis for the conservation of biodiversity and ecological restoration of plant communities in the study area. Based on survey data from 87 sample plots in southwestern Xizang, in this study, two-way indicator species analysis (TWINSPAN) and canonical correspondence analysis (CCA) were employed for quantitative classification and ordination purposes, respectively. Additionally, the diversity of the classified community types obtained was analyzed, along with the factors influencing them. The results indicated that: a total of 295 species of vascular plants belonging to 171 genera and 61 families were recorded in the 87 sample plots; according to TWINSPAN classification, the plants in southwestern Xizang were divided into 17 associations, with the vegetation types being dominated by tussock-forming grass alpine steppes and tussock-forming Kobresia alpine meadows; CCA ordination revealed that the annual average temperature, annual precipitation, and altitude exhibited significant explanatory power; both the α- and β-diversity indices of the coniferous forest community type were the highest, indicating notable community stability; and annual average temperature and annual precipitation significantly affected plant diversity, while the altitude was negatively correlated with the above diversity indices. In summary, the temperature and precipitation were the main environmental factors influencing the composition and distribution of plant communities in southwestern Xizang. The research results could provide a theoretical basis for further investigation and conservation of plant diversity as well as ecological restoration in southwestern Xizang. Full article
(This article belongs to the Section Plant Diversity)
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26 pages, 4605 KB  
Article
Vegetation–Atmosphere Water Deficit as the Primary Control on Alpine Steppe and Forest Coverage: An Empirical Assessment from the Altay Mountains, Northwestern China
by Qiao Xu, Yan Xu, Dong Cui, Tao Lin, Zhiguo Miao, Yincheng Gong, Aishajiang Aili and Fabiola Bakayisire
Biology 2026, 15(11), 879; https://doi.org/10.3390/biology15110879 - 2 Jun 2026
Viewed by 326
Abstract
Mountain vegetation in dryland regions is highly sensitive to climatic variability, particularly changes in water availability and atmospheric demand. This study assessed the relationships between vegetation coverage and climatic factors in the Chinese Altay Mountains from 2000 to 2024 using MODIS NDVI data, [...] Read more.
Mountain vegetation in dryland regions is highly sensitive to climatic variability, particularly changes in water availability and atmospheric demand. This study assessed the relationships between vegetation coverage and climatic factors in the Chinese Altay Mountains from 2000 to 2024 using MODIS NDVI data, meteorological observations, drought indices, and extreme climate indicators. Pixel-based correlation analysis and directional interaction classification were used to evaluate the spatial consistency and divergence between vegetation dynamics and climate variability. The results showed that water availability was the dominant factor controlling vegetation cover. Annual precipitation, SPEI, and precipitation-related extreme indices were generally positively associated with vegetation coverage, whereas warmth-related indices such as GSL, WSDI, and TX90 were mostly negatively associated with vegetation coverage. Temperature showed a spatially variable effect, with warming tending to suppress vegetation in water-limited low- and middle-elevation areas but potentially benefiting vegetation in cold-limited high-elevation zones. SPEI showed a more consistent relationship with vegetation coverage than TVDI, indicating that cumulative climatic water balance better captured regional vegetation drought responses than surface dryness alone. These findings highlight the importance of vegetation–atmosphere water deficit in regulating mountain vegetation dynamics and provide a scientific basis for ecological conservation and water resource management in the Altay Mountains. Full article
(This article belongs to the Section Ecology)
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18 pages, 2853 KB  
Article
Environmental Heterogeneity Drives Distinct Spatial Distribution Patterns of Microbial Co-Occurring Species Across Different Grassland Types
by Wenjing Liu, Kai Xue, Biao Zhang, Shutong Zhou, Weiwei Cao, Kui Wang, Yanbin Hao, Xiaoyong Cui and Yanfen Wang
Microorganisms 2026, 14(1), 156; https://doi.org/10.3390/microorganisms14010156 - 10 Jan 2026
Cited by 2 | Viewed by 855
Abstract
Grasslands, as dominant terrestrial ecosystems, significantly influence soil microbial communities through alterations in soil properties. However, their effects on spatial patterns of soil microbial communities are still under-investigated. To address this, we quantified taxa–area (TAR) and node–area (NAR) relationships for prokaryotic and fungal [...] Read more.
Grasslands, as dominant terrestrial ecosystems, significantly influence soil microbial communities through alterations in soil properties. However, their effects on spatial patterns of soil microbial communities are still under-investigated. To address this, we quantified taxa–area (TAR) and node–area (NAR) relationships for prokaryotic and fungal communities across temperate steppe (TS), alpine steppe (AS), and alpine meadow (AM). Our findings indicated that the spatial turnover of both prokaryotic and fungal communities were higher in alpine steppe and alpine meadow than in temperate steppe, mirroring the gradient of soil environmental heterogeneity. Notably, overall species richness increased logarithmically with sampling area in all grasslands; in striking contrast, co-occurring richness exhibited an increasing and then decreasing trend in AS and AM, but declined monotonically in TS, indicating that microbial interaction networks collapse once a critical spatial threshold is exceeded regulated by ecosystem type and environmental heterogeneity. In growing season, the stochastic dominance in prokaryotic assembly (Normalized stochasticity ratio = 0.71–0.89) and deterministic dominance in fungal assembly (Normalized stochasticity ratio = 0.23–0.37) can be explained by their differences in niche breadth and migration rate. These scale-dependent biogeographic patterns demonstrate that grassland type impacts distinct interactions and spatial patterns of microbial communities. These findings provide novel insights into a comprehensive understanding of how grassland type mediates soil microbial community. Full article
(This article belongs to the Section Environmental Microbiology)
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26 pages, 18192 KB  
Article
Combining In Situ and Remote-Sensing Data to Assess the Spatial Pattern and Changes of Major Grassland Types in Xinjiang, China, Under Climate Change Scenarios
by Jin Zhao, Kaihui Li, Qianying Shao, Jie Bai, Yanming Gong and Yanyan Liu
Remote Sens. 2026, 18(1), 152; https://doi.org/10.3390/rs18010152 - 3 Jan 2026
Cited by 1 | Viewed by 829
Abstract
Examining the long-term spatiotemporal distribution of grassland types and their transitions is crucial for better understanding regional and global changes. Most research in this field has examined the spatial distribution, temporal dynamics of grasslands, and their causes as a unified entity. This study [...] Read more.
Examining the long-term spatiotemporal distribution of grassland types and their transitions is crucial for better understanding regional and global changes. Most research in this field has examined the spatial distribution, temporal dynamics of grasslands, and their causes as a unified entity. This study predicted the distribution of nine major grassland types in Xinjiang under three climate change scenarios from 2041 to 2100 based on 1980s grassland maps, field data in 2023, and 28 factors. The total area of the nine grassland types showed a decreasing trend from 2041 to 2100. The lowland meadow (LM), temperate meadow steppe (TMS), temperate steppe desert (TSD), temperate desert steppe (TDS), and mountain meadow (MM) expanded, while significant declines occurred in alpine meadow (AM), alpine steppe (AS), temperate desert (TD), and temperate steppe (TS). Among cumulative contribution rate of the 28 factors examined in this study, NDVI, vegetation type, slope, elevation, soil_symbol, soil_ph, Bio1, Bio5, Bio8, Bio9, Bio10, Bio12, Bio13, Bio15, and Bio18 played important roles in most grassland types. LM, TD, and AS grassland were found to be more sensitive to E (environment), while AM, TDS, and TSD were more influenced by T (temperature). The distributions of MM and TMS are significantly influenced by the combined effects of all three categories of factors. For TS, the impacts of both temperature and environmental factors are substantial. These findings provided a robust foundation for conservation planning and the sustainable management of grassland ecosystems in temperate and alpine regions. Full article
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20 pages, 21151 KB  
Article
Fusing Enhanced Flux Measurements and Multi-Source Satellite Observations to Improve GPP Estimation for the Qinghai–Tibet Plateau Based on AutoML Techniques
by Mengyao Zhao, Ying Yang, Guoyong Weng, Wei He, Hua Yang, Ngoc Tu Nguyen, Jianqiong Wang, Shuai Liu, Jiayi Chen, Xinhui Lei, Teng Ma, Ziyi Huang and Peipei Xu
Remote Sens. 2026, 18(1), 130; https://doi.org/10.3390/rs18010130 - 30 Dec 2025
Cited by 1 | Viewed by 948
Abstract
The Qinghai–Tibet Plateau (QTP) plays a crucial role in the terrestrial carbon cycle, but the gross primary productivity (GPP) estimates for the region remain highly uncertain due to limited flux observations and modeling challenges. Here, we integrated 65.2 site years of eddy covariance [...] Read more.
The Qinghai–Tibet Plateau (QTP) plays a crucial role in the terrestrial carbon cycle, but the gross primary productivity (GPP) estimates for the region remain highly uncertain due to limited flux observations and modeling challenges. Here, we integrated 65.2 site years of eddy covariance data from 19 flux sites with multi-source remote sensing observations to develop a data driven GPP model for the QTP. Eleven machine learning algorithms from two automated machine learning (AutoML) platforms, H2O AutoML and FLAML, were evaluated to construct an ensemble model named AutoML. The model showed strong performance at site-level across alpine meadow, steppe, wetland, and shrub ecosystems, achieving R2 up to 0.95 and RMSE as low as 0.42 g C m−2 d−1. By validating extracted site-level GPP values from the upscaling GPP datasets against with flux observations, AutoML-GPP demonstrates overall superior or equivalent performance over global GPP products (FLUXCOM X-base, GOSIF, and FluxSat). Regional upscaling estimated a mean annual total GPP of 374.20 Tg C yr−1 from 2002 to 2018, with a slight upward trend of 0.08 Tg C yr−1. Spatially, higher GPP occurred mainly in the eastern QTP, with anomalies linked to climate extremes in 2008, 2010, and 2015. AutoML-GPP effectively captures climate-induced interannual anomalies in the QTP’s GPP, coinciding with GOSIF-GPP and FluxSat GPP, and outperforming the recent released well-known global upscaling flux dataset FLUXCOM X-base. This study provides improved GPP estimation for the QTP, offering new insights into carbon cycling and climate–vegetation interactions. Full article
(This article belongs to the Section Ecological Remote Sensing)
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24 pages, 5699 KB  
Article
Alpine Grassland Growth and Its Ecological Responses to Environmental Impacts: Insights from a Comprehensive Growth Index and SHAP-Based Analysis
by Yanying Li, Yongmei Liu, Xiaoyu Li, Junjuan Yan, Yuxin Du, Ying Meng and Jianhong Liu
Plants 2026, 15(1), 93; https://doi.org/10.3390/plants15010093 - 27 Dec 2025
Viewed by 1360
Abstract
The alpine grassland is one of the most representative ecosystems on the Qinghai–Tibet Plateau, Growth monitoring is fundamental for the alpine grassland maintenance and husbandry sustainability. In this study, by the integration of regression model, principal component analysis, and SHAP-enhanced machine learning, a [...] Read more.
The alpine grassland is one of the most representative ecosystems on the Qinghai–Tibet Plateau, Growth monitoring is fundamental for the alpine grassland maintenance and husbandry sustainability. In this study, by the integration of regression model, principal component analysis, and SHAP-enhanced machine learning, a comprehensive growth index (CGI) was proposed for the accurate and quick assessment of alpine grassland growth in Qinghai Province, located in the eastern Qinghai–Tibet Plateau. The temporal and spatial growth behaviors of the main grassland types over 2001–2023 were then determined and the differences in key driving factors and their responses explored. The results indicated that the CGI composed of KNDVI, EVI, MSAVI, GNDVI and CVI characterized the typical ecological and physical parameters related to grassland growth, proved to be optimal and efficient in long-term growth monitoring. Alpine grassland growth fluctuated but gradually increased from 2001 to 2023, but individual types exhibited different trends. In particular, the two main types of alpine meadow and alpine steppe displayed the weakest increasing trend in growth, with the good-growth and continuous-increasing area proportions of 26.01% and 18.03%, 70.45% and 74.72%, respectively. Soil total nitrogen was the most critical common factor and significantly increased the growth across all five grassland types, then followed by grazing intensity and precipitation, which exhibits diverse effects on the individual types. The result implies the significant heterogeneity in the key driviers which affect the alpine grassland growth over large scale. Full article
(This article belongs to the Section Plant Ecology)
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21 pages, 3816 KB  
Article
Discrepant Pathway in Regulating ET Under Change in Community Composition of Alpine Grassland in the Source Region of the Yellow River
by Shuntian Guan, Longyue Zhang, Yunqi Xiong, Congjia Li, Zhenzhen Zheng, Shibo Huang, Ronghai Hu, Xiaoming Kang, Jianqin Du, Kai Xue, Xiaoyong Cui, Yanfen Wang and Yanbin Hao
Remote Sens. 2025, 17(24), 4046; https://doi.org/10.3390/rs17244046 - 17 Dec 2025
Viewed by 602
Abstract
Understanding evapotranspiration (ET) dynamics under community composition transitions in grasslands is crucial for interpreting alpine ecosystem responses to climate change. We investigated variations in ET and its components during the growing season across five alpine grassland transition types in the Source Region of [...] Read more.
Understanding evapotranspiration (ET) dynamics under community composition transitions in grasslands is crucial for interpreting alpine ecosystem responses to climate change. We investigated variations in ET and its components during the growing season across five alpine grassland transition types in the Source Region of the Yellow River (SRYR) from 1986 to 2018, integrating climatic, vegetation, and soil factors. Under warming and wetting conditions, ET increased significantly by 1.17 mm yr−1, accounting for 79.39% of annual precipitation, while soil moisture declined slightly. A pronounced temperature–precipitation decoupling emerged between alpine meadow-origin (AM-origin) and alpine steppe-origin (AS-origin) transitions, indicating differential hydrological responses driven by community composition. Vegetation growth increased across all transitions, yet its regulation of ET components varied by transition type. Transpiration dominated ET increases, contributing over 80% in AM-origin and 100% in AS-origin transitions. Soil evaporation exhibited contrasting trends: decreasing in AS-origin transitions due to enhanced soil insulation from vegetation growth, but increasing in AM-origin transitions, thereby reducing soil moisture. Interannual ET growth rates and seasonal fluctuations were greater in AM-origin than in AS-origin transitions. A critical turning point in ET trends, caused by changes in precipitation, revealed the divergent hydrological trajectories among the transitions. In AM-origin transitions, temperature primarily drove ET increases, causing soil drying (strongest in AM to TS), whereas in AS-origin transitions, precipitation dominated, resulting in soil wetting (more pronounced in AS to AM). These findings demonstrate that the directionality of compositional transitions governs hydrological responses more strongly than absolute vegetation states. Full article
(This article belongs to the Section Ecological Remote Sensing)
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17 pages, 7577 KB  
Article
Increasing Variability in Precipitation Impacts Alpine Rangeland Grazing Across Tibet
by Cheng Duan, Jin Huang, Yang Yu, Xuejie Mou, Huixia Chai and Xiahui Wang
Sustainability 2025, 17(24), 11159; https://doi.org/10.3390/su172411159 - 12 Dec 2025
Cited by 1 | Viewed by 671
Abstract
Increasing precipitation variability has been tightly coupled to livestock grazing through direct impacts on vegetation productivity in rangeland ecosystems. However, where and to what extent such impacts occur has not been quantified systematically at the regional scale; thus, adaptive grazing management is plagued [...] Read more.
Increasing precipitation variability has been tightly coupled to livestock grazing through direct impacts on vegetation productivity in rangeland ecosystems. However, where and to what extent such impacts occur has not been quantified systematically at the regional scale; thus, adaptive grazing management is plagued by this knowledge gap. Hence, using 20 years of precipitation, rangeland productivity, and livestock density data across Tibet, we assessed long-term precipitation variability impacts on alpine rangeland grazing, specifically highlighting variations in intra- and inter-annual precipitation variability. We showed that the precipitation concentration index (PCI) and coefficient of variation in precipitation (CVP) both increased significantly across Tibet over the past two decades, especially in the western region. On the contrary, grazing intensity (GI) in most rangeland areas markedly declined over the same period. Moreover, we found that GI is highly responsive to PCI and CVP for the alpine steppe, but interestingly, only PCI is significantly associated with GI for the alpine meadow. Furthermore, the Granger causality test indicates an extremely significant causality between GI and PCI, further highlighting that PCI was a remarkable determinant of rangeland grazing over the last two decades. Notably, we statistically identified rangelands with higher precipitation variability that experienced intensive livestock grazing, specifically, GI responded positively to CVP and PCI. In conclusion, our findings provide novel support for the increasing precipitation variability impacts on rangeland grazing over time across Tibet, especially the intra-annual variation. Thus, we advocate the implementation of adaptive grazing management, such as excluding and minimizing grazing for the alpine rangeland ecosystem under higher precipitation variability. Full article
(This article belongs to the Special Issue Sustainable Management: Plant, Biodiversity and Ecosystem)
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19 pages, 8957 KB  
Article
Mean Annual Temperature, Soil Organic Matter and Phyllospheric Bacterial Diversity Shape Biomass of Dominant Species Along a Degradation Gradient in Alpine Steppes: A Case Study from the Qinghai–Tibet Plateau
by Kaifu Zheng, Xin Jin, Jingjing Li and Guangxin Lu
Microorganisms 2025, 13(12), 2787; https://doi.org/10.3390/microorganisms13122787 - 7 Dec 2025
Viewed by 652
Abstract
The structure and function of alpine steppes are maintained largely by dominant species, which in turn determine the productivity and stability of plant communities. Nutrient acquisition and stress regulation may, to some extent, be mediated by phyllospheric microbiota at the interface of plants [...] Read more.
The structure and function of alpine steppes are maintained largely by dominant species, which in turn determine the productivity and stability of plant communities. Nutrient acquisition and stress regulation may, to some extent, be mediated by phyllospheric microbiota at the interface of plants with the atmosphere, and phyllospheric microbes are capable of amplifying and transmitting vegetation responses to degradation. Previous research has mainly addressed climate, soil, vegetation and soil microbiota or has assessed phyllosphere communities as a whole, thereby overlooking the specific responses of phyllospheric bacteria associated with the vegetation-dominant species Stipa purpurea along gradients of vegetation degradation in alpine steppes. In this study, we characterised vegetation degradation at the community level (from non-degraded to severely degraded grasslands) and quantified associated changes in the dominant species Stipa purpurea (cover, height and aboveground biomass) and its phyllospheric bacterial communities, in order to elucidate response patterns within the coupled system of host plants, phyllosphere microbiota, climate (mean annual temperature and precipitation) and soil physicochemical properties. Compared with non-degraded (ND) grasslands, degraded sites had a 22.6% lower mean annual temperature (MAT) and reductions in total nitrogen, nitrate nitrogen, organic matter (OM) and soil quality index (SQI) of 49.4%, 55.6%, 46.8% and 47.6%, respectively. Plant community cover and the aboveground biomass of dominant species declined significantly with increasing degradation. Along the vegetation-degradation gradient from non-degraded to severely degraded alpine steppes, microbial source-tracking analysis of the phyllosphere of the dominant species Stipa purpurea revealed a sharp decline in the contribution of phyllospheric bacterial sources. Estimated contributions from non-degraded sites to lightly, moderately and severely degraded sites were 95.68%, 62.21% and 6.89%, respectively, whereas contributions from lightly to moderately degraded and from moderately to severely degraded sites were 34.89% and 16.47%, respectively. Bacterial richness increased significantly, and β diversity diverged under severe degradation (PERMANOVA, F = 5.48, p < 0.01). From light to moderate degradation, biomass and relative cover of the dominant species decreased significantly, while the phyllosphere bacterial community appeared more strongly influenced by the host than by environmental deterioration; the community microbial turnover index (CMTB) and microbial resistance potential increased slightly but non-significantly (p > 0.05). Under severe degradation, worsening soil conditions and hydrothermal regimes exerted a stronger influence than the host, and CMTB and microbial resistance potential decreased by 6.5% and 34.1%, respectively (p < 0.05). Random-forest analysis indicated that climate, soil, phyllosphere diversity and microbial resistance jointly accounted for 42.1% of the variation in constructive-species biomass (R2 = 0.42, p < 0.01), with the remaining variation likely driven by unmeasured biotic and abiotic factors. Soil contributed the most (21.73%), followed by phyllosphere diversity (9.87%) and climate (8.62%), whereas microbial resistance had a minor effect (1.86%). Specifically, soil organic matter (OM) was positively correlated with biomass, whereas richness, beta diversity and MAT were negatively correlated (p < 0.05). Taken together, our results suggest that under ongoing warming on the Qinghai–Tibet Plateau, management of alpine steppes should prioritise grasslands in the early stages of degradation. In these systems, higher soil organic matter is associated with greater phyllospheric microbial resistance potential and increased biomass of Stipa purpurea, which may help stabilise this dominant species and slow further vegetation degradation. Full article
(This article belongs to the Section Environmental Microbiology)
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27 pages, 6095 KB  
Article
Cumulative and Lagged Drought Effects Shape Start and End of Season on the Mongolian Plateau
by Yilin Liu, Yu Wang, Maolin Li, Qi Shi, Xinyu Yang, Bowen Chi, Ji Long, Qiang Yu, Buyanbaatar Avirmed, Orgilbold Myangan, Ganbold Bayanmunkh and Dambadarjaa Naranbat
Forests 2025, 16(12), 1814; https://doi.org/10.3390/f16121814 - 4 Dec 2025
Cited by 1 | Viewed by 985
Abstract
Dryland phenology is tightly constrained by water availability, yet the temporal depth of drought influence remains poorly resolved at regional scales. We analyzed the start and end of season across the Mongolian Plateau using 500 m MODIS kNDVI for 2001–2020 and a phenology-anchored [...] Read more.
Dryland phenology is tightly constrained by water availability, yet the temporal depth of drought influence remains poorly resolved at regional scales. We analyzed the start and end of season across the Mongolian Plateau using 500 m MODIS kNDVI for 2001–2020 and a phenology-anchored framework that linked multi-timescale SPEI directly to the month of each phenological event. By varying accumulation windows and testing month-wise lags up to twelve months, we mapped pixel-level optimal timescales and sensitivities. Phenology exhibits a clear north–south gradient with weak long-term shifts relative to large interannual variability. Drought acts through two pathways. Multi-month winter–spring moisture deficits delay spring green-up, with the strongest SOS sensitivity to antecedent drought about six to nine months prior. Summer–autumn dryness advances dormancy, and EOS is governed mainly by near-term moisture over the previous one to two months. Responses differ among ecoregions, with deserts and desert steppes the most sensitive and forests and alpine meadows less responsive. These asymmetric timescales imply that prolonged deficits can postpone spring emergence into the following year, whereas short deficits truncate the current season, offsetting warming-driven extensions of growing-season length. Incorporating phenology-anchored, multi-timescale drought indicators can improve model forecasts of dryland carbon–water dynamics and inform monitoring and adaptation in the most water-limited ecoregions. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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24 pages, 8222 KB  
Article
Soil Phosphorus Fraction Characteristics in Different Alpine Grassland Types of the Qinghai–Tibet Plateau
by Xueting Li, Zhan Liu, Cuilan Li, Ning Zong, Nianpeng He, Zhiyuan Cao and Jinjing Zhang
Agronomy 2025, 15(12), 2689; https://doi.org/10.3390/agronomy15122689 - 22 Nov 2025
Viewed by 1078
Abstract
The alpine grassland ecosystem of the Tibetan Plateau is a vital base for animal husbandry and a key ecological security barrier in China. Phosphorus (P), an essential nutrient, is among the primary factors limiting grassland productivity. However, the spatial distribution of soil P [...] Read more.
The alpine grassland ecosystem of the Tibetan Plateau is a vital base for animal husbandry and a key ecological security barrier in China. Phosphorus (P), an essential nutrient, is among the primary factors limiting grassland productivity. However, the spatial distribution of soil P fractions across alpine grasslands on the Tibetan Plateau and their environmental drivers remain unclear, limiting our understanding of P cycling and grassland productivity. This study examined the composition and distribution of soil P in three representative alpine grasslands (meadow, steppe, and desert) using a combination of chemical fractionation and 31P nuclear magnetic resonance (NMR) spectroscopy. The results revealed pronounced spatial heterogeneity, with total soil P content varying by approximately 2.4-fold among the grassland types. Alpine meadows had the highest total P (0.73 g kg−1) and available P (4.02 mg kg−1) concentrations, with the latter being nearly twice that of alpine steppes and deserts. Alpine meadows were characterized by a predominance of labile and moderately labile organic P (e.g., NaOH-Po) and a diverse array of phosphate monoesters and diesters, whereas alpine deserts were dominated by stable, calcium-bound inorganic P (HCl-Pi). Temperature, precipitation, pH, and phosphatase activity were identified as key factors regulating the distribution and transformation of P fractions. The distinct P fractions and availability uncovered in this study are essential for predicting grassland ecosystem responses to environmental change and guiding sustainable pasture management on the Tibetan Plateau. Full article
(This article belongs to the Section Grassland and Pasture Science)
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18 pages, 21941 KB  
Article
Phenological Shifts of Vegetation in Seasonally Frozen Ground and Permafrost Zones of the Qinghai–Tibet Plateau
by Tianyang Fan, Xinyan Zhong, Chong Wang, Lingyun Zhou and Zhinan Zhou
Remote Sens. 2025, 17(19), 3391; https://doi.org/10.3390/rs17193391 - 9 Oct 2025
Cited by 2 | Viewed by 1350
Abstract
Vegetation phenology serves as a crucial indicator reflecting vegetation responses to the growth environment and climate change. Existing studies have demonstrated that in permafrost regions, the impact of frozen soil changes on vegetation phenology is more direct and pronounced compared to climate factors. [...] Read more.
Vegetation phenology serves as a crucial indicator reflecting vegetation responses to the growth environment and climate change. Existing studies have demonstrated that in permafrost regions, the impact of frozen soil changes on vegetation phenology is more direct and pronounced compared to climate factors. Amid the slowdown of global warming in the 21st century, permafrost dynamics continued to drive uncertain variations in vegetation phenological stages across the Qinghai–Tibet Plateau (QTP). Using MODIS Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) data during 2001–2024, this study derived vegetation phenological parameters and analyzed their spatiotemporal patterns on the QTP. The results indicate that overall, the start of growing season (SOS) was advanced, the end of growing season (EOS) was delayed, and the length of growing season (LOG) was extended throughout the study period. Additionally, divergent phenological trends were observed across three distinct phases, and regarding frozen soil types, vegetation phenology in permafrost and seasonally frozen ground regions exhibited distinct characteristics. From 2001 to 2024, both permafrost and seasonally frozen ground regions showed an advanced SOS and prolonged LOG, but significant differences were observed in EOS dynamics. For vegetation types, alpine meadow displayed advanced SOS and EOS, alongside an extended LOG. The alpine steppe exhibited advanced SOS and delayed EOS with an extended LOG. Alpine desert displayed SOS advancement and EOS delay, alongside LOG extension. These findings revealed variations in vegetation phenological changes under different frozen soil types and highlighted divergent responses of distinct frozen soil types to climate change. They suggested that the influence of frozen soil types should be considered when investigating vegetation phenological dynamics at the regional scale. Full article
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18 pages, 7245 KB  
Article
Nitrogen Enrichment Reshapes Contrasting Microbial Networks in Northern Tibetan Alpine Meadow vs. Steppe
by Xueying Chen, Peili Shi, Jialuo Yu, Ge Hou, Ning Zong and Huixin Hei
Plants 2025, 14(17), 2803; https://doi.org/10.3390/plants14172803 - 7 Sep 2025
Cited by 1 | Viewed by 1524
Abstract
Increased Nitrogen (N) input exerts significant impact on the functional integrity of terrestrial ecosystems, with alpine grasslands being particularly susceptible. Soil microbes are intricately intertwined with nearly all facets of essential biogeochemical cycle, underscoring their pivotal role in ecosystem processes. To elucidate how [...] Read more.
Increased Nitrogen (N) input exerts significant impact on the functional integrity of terrestrial ecosystems, with alpine grasslands being particularly susceptible. Soil microbes are intricately intertwined with nearly all facets of essential biogeochemical cycle, underscoring their pivotal role in ecosystem processes. To elucidate how N enrichment modulates soil microbes and their diversity, 11-year N addition experiments were conducted in a semi-humid alpine meadow (AM) and an arid alpine steppe (AS) on the Northern Tibetan Plateau. We measured soil properties, aboveground net primary productivity (ANPP), plant diversity, microbial composition and diversity, as well as microbial co-occurrence networks. The results revealed that N additions profoundly reshaped microbial co-occurrence in alpine grasslands, albeit via divergent mechanisms in different ecosystems. In AM, N enrichment destabilized microbial networks mainly through reduced bacterial diversity linked to plant diversity loss. Conversely, in the harsher AS, N addition fostered closer microbial interactions, forming a more stable co-occurrence network despite lower plant richness, predominantly attributed to increased soil nutrient availability. Our results highlight the significance of co-occurrence networks as a key component of microbial biodiversity and emphasize the imperative of deciphering microbial interaction mechanisms to unravel soil functional dynamics under global nitrogen enrichment. Full article
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17 pages, 2157 KB  
Article
Effects of Fertilization and Reseeding on Above- and Belowground Biodiversity in Degraded Alpine Steppe
by Xiaochun Ning, Shouxing Wang, Dongzhi Huangqing, Yanbin Kang, Yafei Zhang, Mingming Shi, Liusheng Yang and Mingxin Yang
Diversity 2025, 17(9), 617; https://doi.org/10.3390/d17090617 - 2 Sep 2025
Cited by 3 | Viewed by 1705
Abstract
The ecological restoration of degraded alpine steppe is a critical component of ecological conservation efforts on the Qinghai–Tibetan Plateau. In this study, we investigated the effects of fertilization, reseeding, and combined fertilization with reseeding restoration measures on the vegetation community, soil properties and [...] Read more.
The ecological restoration of degraded alpine steppe is a critical component of ecological conservation efforts on the Qinghai–Tibetan Plateau. In this study, we investigated the effects of fertilization, reseeding, and combined fertilization with reseeding restoration measures on the vegetation community, soil properties and microbial community diversity in degraded alpine steppe through field vegetation surveys, and soil microbial high-throughput sequencing at an experimental site of fertilized and reseeded grassland restoration located in the Yellow River Source area. The results demonstrated the following: (1) both reseeding and combined fertilization with reseeding restoration measures significantly affected grassland vegetation community structure and diversity; (2) fertilization and combined fertilization with reseeding restoration measures significantly affected soil pH and total phosphorus (TP) content; (3) while fertilization and combined fertilization with reseeding restoration measures markedly altered microbial community structure, reseeding alone significantly affected microbial diversity. Co-occurrence network analysis revealed that soil microbial communities were significantly influenced by fertilization restoration measures; redundancy analysis (RDA) showed that microbial communities under fertilization and combined fertilization with reseeding restoration measures were primarily governed by soil TP, whereas those in control and reseeding plots were strongly associated with soil pH and organic carbon (SOC). This study explored effective restoration measures suitable for degenerating alpine steppe in the Yellow River Source area, aiming to provide a scientific basis and technical support for the ecological protection and restoration of the Three-River Headwaters. Full article
(This article belongs to the Special Issue Ecology and Restoration of Grassland—2nd Edition)
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Article
Seed Size-Number Trade-Off Exists in Graminoids but Not in Forbs or Legumes: A Study from 11 Common Species in Alpine Steppe Communities
by Xiaolong Zhou, Ronghua Duan, Jian Long and Haiyan Bu
Plants 2025, 14(17), 2730; https://doi.org/10.3390/plants14172730 - 2 Sep 2025
Cited by 1 | Viewed by 2147
Abstract
Seed size and number are two important components of plant reproductive traits. Previous theoretical studies have suggested that resource limitations lead to a strong trade-off between seed size and seed number. However, empirical evidence from natural communities remains scarce. In this study, the [...] Read more.
Seed size and number are two important components of plant reproductive traits. Previous theoretical studies have suggested that resource limitations lead to a strong trade-off between seed size and seed number. However, empirical evidence from natural communities remains scarce. In this study, the relationship between seed size and seed number was tested at the community level and in three functional groups—graminoids, forbs, and legumes—in a natural alpine steppe community in the Tianshan Mountains. The role of limiting resources in reproduction and in determining trade-off patterns was also examined by treating the reproductive biomass and allocation of each species as a resource pool for producing seeds. Our results showed a significant negative relationship between seed size and seed number at the community level, which indicated that a trade-off between seed size and number existed and that the species that produced large seeds produced fewer seeds and vice versa. This trade-off was detected for the graminoid group but not for the forb or legume group, so the trade-off at the community level was determined primarily by graminoid species. Moreover, the graminoid group had lower reproductive biomass and allocation than the forb and legume groups, indicating that the graminoid species were more strictly limited by reproductive resources. Our study provides evidence of a seed size-number trade-off in a natural alpine steppe community, especially among graminoid species, and the important role of reproductive resources in determining the trade-off. Full article
(This article belongs to the Section Plant Ecology)
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