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Search Results (1,010)

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45 pages, 3600 KB  
Review
Application of Artificial Intelligence and Machine Learning in Vertical Farming: A Comprehensive Review
by Mi Young Kim, Geunwoo Park and Chang Ho Seo
Sustainability 2026, 18(16), 8261; https://doi.org/10.3390/su18168261 - 12 Aug 2026
Viewed by 127
Abstract
Vertical farming (VF) offers a smart way to grow crops in stacked layers inside controlled indoor environments. By doing so, it uses far less land and water than traditional open-field agriculture, making it a promising solution for cities with limited space and resources. [...] Read more.
Vertical farming (VF) offers a smart way to grow crops in stacked layers inside controlled indoor environments. By doing so, it uses far less land and water than traditional open-field agriculture, making it a promising solution for cities with limited space and resources. In recent years, artificial intelligence (AI), machine learning (ML), and Internet of Things (IoT) technologies have begun to transform vertical farming. These tools are moving the industry away from rigid, rule-based systems toward more flexible, data-driven operations that can adapt in real time. This paper presents a systematic review of 208 peer-reviewed studies from 2015 to 2025. It explores how AI, ML, and IoT are applied across the VF ecosystem, focusing on key areas such as computer vision for disease detection, crop growth and yield prediction, smart climate control, and precision nutrient and irrigation management. This review examines the performance of different algorithms, including Convolutional Neural Networks (CNNs), Random Forest, XGBoost, and LSTMs across hydroponic, aeroponic, and aquaponic systems. The review also covers IoT setups with multi-sensor networks, edge-cloud computing, and automated control systems. Commercial farms have shown real gains in resource efficiency and shorter supply chains. However, challenges remain: high energy use (especially from LED lighting, which makes up 40–60% of costs), expensive setup, scattered datasets, and limited real-world testing. Many high-accuracy claims (>95%) come from lab conditions and need better validation in actual farms. Overall, AI-powered vertical farming has strong potential to support resilient urban food systems. Future work should focus on lightweight edge AI models, improved data standards, explainable AI, and robust life cycle assessments to ensure the benefits outweigh the environmental and economic costs. Full article
(This article belongs to the Special Issue Precision Farming Practices for Sustainable Plant Protection)
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19 pages, 3485 KB  
Article
Estimating Oilseed Rape Canopy Water Content Using UAV Multispectral Imagery and Machine Learning: A Comparative Evaluation of Feature Selection Strategies Across Two Growing Seasons
by Hao Hu, Wanzhu Ma, Hongkui Zhou, Zhiqing Zhuo, Kangying Zhu, Dong Li, Ailian Zhou, Jiajia Liu and Shuijin Hua
Remote Sens. 2026, 18(16), 2707; https://doi.org/10.3390/rs18162707 - 12 Aug 2026
Viewed by 121
Abstract
Accurate estimation of canopy water content (OWC) is essential for precision irrigation, crop growth monitoring, and yield prediction. Unmanned aerial vehicle (UAV)-based multispectral remote sensing provides a rapid and non-destructive approach for monitoring crop water status; however, the selection of effective spectral features [...] Read more.
Accurate estimation of canopy water content (OWC) is essential for precision irrigation, crop growth monitoring, and yield prediction. Unmanned aerial vehicle (UAV)-based multispectral remote sensing provides a rapid and non-destructive approach for monitoring crop water status; however, the selection of effective spectral features and appropriate machine learning algorithms for robust OWC estimation remains insufficiently investigated, particularly across multiple growing seasons. This study evaluated the potential of UAV multispectral imagery for estimating oilseed rape canopy water content using two feature selection strategies and four representative machine learning algorithms. Field experiments were conducted during two consecutive growing seasons (2023–2024 and 2024–2025). Different sowing dates, nitrogen application rates, and planting densities were used to create a broad range of canopy water conditions. UAV multispectral images were acquired at ten representative growth stages during the reproductive period, from stem elongation to physiological maturity. Fourteen vegetation indices (VIs) were extracted from the multispectral imagery. Pearson correlation analysis and principal component analysis (PCA) were used to select informative features. These features were then used to develop multiple linear regression (MLR), partial least squares (PLS), support vector machine (SVM), and random forest (RF) models. Model performance was evaluated using each single-year dataset and the combined two-year dataset to assess robustness under different seasonal conditions. The RF model consistently achieved the highest prediction accuracy. The correlation-based RF model developed from the combined two-year dataset produced the best performance. It achieved an R2 of 0.966, an RMSE of 1.734%, and an RRMSE of 2.360% for the training dataset. For the independent testing dataset, the corresponding values were 0.901, 2.794%, and 3.830%, respectively. The PCA-based models showed similar performance and effectively reduced feature redundancy. However, they did not consistently outperform the correlation-based models. These results indicate that combining UAV multispectral imagery with appropriate feature selection and machine learning algorithms can accurately estimate oilseed rape canopy water content under field conditions. Integrating data from multiple growing seasons further improves model robustness and provides a practical basis for UAV-assisted crop water monitoring and precision agricultural management. Full article
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27 pages, 3030 KB  
Article
Durum Wheat Yield Prediction: A Machine Learning Framework Integrating Sentinel-2 Imagery and Meteorological Data
by Maria Bebie and Aris Kyparissis
Remote Sens. 2026, 18(16), 2694; https://doi.org/10.3390/rs18162694 - 11 Aug 2026
Viewed by 162
Abstract
Accurate crop yield prediction provides essential information for strategic decision-making and precision agriculture, yet achieving reliable forecasts across unseen growing seasons remains challenging. The main objective of this study is to develop a two-stage data assimilation framework that integrates multi-scale environmental data to [...] Read more.
Accurate crop yield prediction provides essential information for strategic decision-making and precision agriculture, yet achieving reliable forecasts across unseen growing seasons remains challenging. The main objective of this study is to develop a two-stage data assimilation framework that integrates multi-scale environmental data to improve yield predictions and overcome the spatial–temporal autocorrelation limitations of standard machine learning (ML) models. Utilizing an eight-year continuous dataset (2018–2025) of durum wheat fields in Thessaly, Greece, this study integrates high-resolution Sentinel-2 multispectral imagery with macro-scale ERA5-Land meteorological variables. Eight ML algorithms are trained to predict yield at the pixel level. To test model generalization and prevent overfitting, the framework is evaluated using both standard random splitting and leave-one-year-out (LOYO) cross-validation. Concurrently, multiple linear regression (MLR) is utilized to select the most significant meteorological predictors from monthly temperature (maximum and minimum) and precipitation data, which are then integrated into the pixel-level predictions via additive and multiplicative late-fusion assimilation. The results demonstrate that, while standard random splitting produces high explained variance (R2 > 0.90), LOYO validation shows a predictive maximum of approximately 50% explained variance. The late-fusion assimilation slightly reduces interannual offsets, from an RMSE of 966 kg ha−1 to 902 kg ha−1. However, the R2 values remain static due to informational saturation. This study concludes that, while integrating regional climate data improves absolute annual yield magnitudes, securing reliable agricultural forecasts requires the integration of localized agronomic metadata, such as soil properties and field-specific management practices. Full article
(This article belongs to the Section Remote Sensing in Agriculture and Vegetation)
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19 pages, 3350 KB  
Article
Fractal-Based Image Analysis for Multi-Stage Detection of Tomato Late Blight Using a Laboratory Image Dataset of Greenhouse-Grown Tomato Plants
by Fazliddin Makhmudov, Jamshid Khamzaev, Mirzaakbar Hudayberdiev, Baxodir Achilov, Shavkat Otamuradov, Takhir Kuchkorov, Islambek Saymanov and Alpamis Kutlimuratov
Horticulturae 2026, 12(8), 979; https://doi.org/10.3390/horticulturae12080979 - 6 Aug 2026
Viewed by 184
Abstract
This paper considers the problem of early detection of late blight (Phytophthora infestans) in tomatoes based on computer vision and machine learning methods. The main purpose of the study was to develop a representative dataset of images of tomato leaves and [...] Read more.
This paper considers the problem of early detection of late blight (Phytophthora infestans) in tomatoes based on computer vision and machine learning methods. The main purpose of the study was to develop a representative dataset of images of tomato leaves and an approach to extracting informative features for classifying the stages of disease development. A new dataset was generated using tomato plants grown under greenhouse conditions, with leaf images subsequently captured under controlled laboratory conditions, including five stages of late blight progression with variability in imaging devices, lighting conditions, and temporal disease dynamics. To improve the quality of image analysis, a preprocessing stage was applied, including conversion to grayscale, median filtering, and binarization using the Otsu method. In addition to the traditional textural features, fractal analysis was used to quantify the structural complexity of the affected leaf areas. To verify the information content of the selected features, classification experiments were conducted using Random Forest, XGBoost, and Support Vector Machine models, and the quality was evaluated using accuracy, precision, recall, and F1-score metrics. The results showed that the combination of textural and fractal features contributes to a more accurate distinction between the stages of disease. The developed dataset and the proposed approach can be used in further research on plant disease diagnosis, agricultural monitoring, and precision farming systems although it should be acknowledged that the dataset is limited to greenhouse settings, and field-scale generalizability requires further validation. Full article
(This article belongs to the Section Plant Pathology and Disease Management (PPDM))
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42 pages, 2119 KB  
Review
Bridging Magnetic Field Agriculture and UAV-Based Precision Monitoring: An Integrated Dual-Stream Evidence Synthesis and Conceptual Framework for Field-Scale Validation
by George Papadopoulos, Evgenia Georgiou, Antonia Oikonomou, Spyros Fountas and Dimitrios Bilalis
Sustainability 2026, 18(15), 7978; https://doi.org/10.3390/su18157978 - 6 Aug 2026
Viewed by 155
Abstract
Magnetic field (MF) technologies have been explored in agriculture since the 1930s, with research activity increasing markedly since 2016. However, they have not achieved mainstream adoption, partly because no MF-specific validated methodology exists for evaluating their effects under realistic field conditions. Unmanned Aerial [...] Read more.
Magnetic field (MF) technologies have been explored in agriculture since the 1930s, with research activity increasing markedly since 2016. However, they have not achieved mainstream adoption, partly because no MF-specific validated methodology exists for evaluating their effects under realistic field conditions. Unmanned Aerial Vehicle (UAV)-based multispectral sensing represents a potential pathway to address this limitation by providing spatially explicit, non-destructive estimates of key canopy physiological variables at field scale, thereby enabling, for the first time, the systematic evaluation and validation of MF treatment responses under open-field conditions. To realise this potential, however, a common evidential basis must first be established by identifying crop physiological variables that are both consistently modulated by MF treatments and reliably detectable by UAV remote sensing. This study addressed this challenge through a dual-stream evidence synthesis of 216 peer-reviewed publications, comprising 102 studies on MF treatments in agricultural crops and 114 studies on UAV-based multispectral monitoring. Evidence from both research domains was synthesised to identify physiological variables that are simultaneously responsive to MF treatments and detectable through UAV remote sensing. Five direct bridge variables were identified: chlorophyll content, nitrogen use efficiency (NUE)/nitrogen assimilation, above-ground biomass (AGB), leaf area index (LAI), and yield. Chlorophyll content emerged as the strongest bridge variable, combining consistent MF responsiveness with UAV estimation accuracies of up to R2 = 0.90. Based on these findings, a conceptual framework was developed linking MF treatments, UAV-derived vegetation indices, ground-truth measurements, and machine-learning approaches for field-scale validation. The review revealed a complete absence of integration between the two research domains within the reviewed corpus, despite their strong biological and methodological compatibility. The proposed framework is conceptual and remains to be experimentally validated; it provides the first operational pathway for evaluating MF technologies under realistic farming conditions and may support future research on sustainable and digitally enabled crop production systems. Full article
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9 pages, 208 KB  
Editorial
Perspectives and Challenges of Machine Learning for Applications in Agriculture and Vegetation Using Remote Sensing
by Christoph Jörges and Aaron Moody
Remote Sens. 2026, 18(15), 2589; https://doi.org/10.3390/rs18152589 - 5 Aug 2026
Viewed by 234
Abstract
Recent advances in Earth observation and machine learning have significantly enhanced the capacity to monitor agricultural systems and terrestrial vegetation across spatial and temporal scales. This editorial synthesizes the contributions of eleven studies published in the Special Issue ‘Machine Learning for Applications in [...] Read more.
Recent advances in Earth observation and machine learning have significantly enhanced the capacity to monitor agricultural systems and terrestrial vegetation across spatial and temporal scales. This editorial synthesizes the contributions of eleven studies published in the Special Issue ‘Machine Learning for Applications in Agriculture and Vegetation Using Remote Sensing’. These contributions highlight emerging methodological trends, as well as persistent challenges in remote sensing for agriculture and vegetation measurement and monitoring, and reflect the growing dominance of deep learning in high-resolution mapping and segmentation. An increasing importance of multi-sensor data fusion, integrating multi- and hyperspectral satellites, UAV, and environmental data, is found. Deep learning is emerging as an effective approach for retrieving key biophysical parameters such as biomass, crop height, and yield. The collected studies also emphasize the critical role of sensor characteristics and scale, particularly the trade-offs between spectral, spatial, and temporal resolution in vegetation analysis. Despite notable progress, several limitations remain. Model transferability across regions and sensors is still constrained and multi-source data integration often lacks standardized frameworks. Empirical approaches still dominate the retrieval of biophysical variables, limiting robustness and physical interpretability. The contributions also reveal a persistent gap between high-resolution, small-scale analyses and their scalability to regional or global applications. Therefore, this editorial argues for a transition towards hybrid modeling approaches that combine physical knowledge with data-driven machine learning methods, the adoption of formal data assimilation frameworks for multi-source integration, and the development of scalable and uncertainty-aware workflows. The broader scientific context of the contributions is given by providing a critical perspective on the current state of the field and outlining the key research directions necessary to advance remote sensing in agriculture and ecosystem monitoring. Full article
53 pages, 715 KB  
Systematic Review
Change Detection in Remote Sensing Imagery: A Systematic Review of Statistical, Machine Learning, and Deep Learning Methods
by Mohammad Jabbarizadegan and Piero Fraternali
Remote Sens. 2026, 18(15), 2573; https://doi.org/10.3390/rs18152573 - 4 Aug 2026
Viewed by 729
Abstract
Change detection (CD) is a fundamental remote sensing task that identifies surface modifications from multi-temporal imagery of the same area, with applications in urban monitoring, agriculture, forest disturbance mapping, disaster assessment, and land cover analysis. The task is complicated by radiometric and atmospheric [...] Read more.
Change detection (CD) is a fundamental remote sensing task that identifies surface modifications from multi-temporal imagery of the same area, with applications in urban monitoring, agriculture, forest disturbance mapping, disaster assessment, and land cover analysis. The task is complicated by radiometric and atmospheric variability, co-registration errors, seasonal cycles, and sensor heterogeneity. Deep learning has progressively superseded traditional and classical machine learning approaches through hierarchical feature extraction and end-to-end optimization. Following the PRISMA 2020 guidelines, this systematic review examines 144 primary studies identified through a structured Scopus search complemented by the authors’ prior research and citation searching, spanning three paradigms: traditional approaches (algebraic operators, transformations, probabilistic frameworks), classical machine learning (support vector machines, random forests, object-based analysis), and deep learning architectures (fully convolutional, Siamese, attention-based, Transformer, state space, diffusion-based, and weakly supervised models). We provide background on problem formulation, benchmark datasets, and evaluation metrics, alongside a taxonomy organized by paradigm and supervision mode. A quantitative comparison on dominant benchmarks reveals the strengths and limitations of current methods. Open challenges include the absence of a universal benchmark protocol, the research-to-deployment gap, and the need for label-efficient learning. This review serves as a structured reference and outlines promising directions for the field. Full article
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19 pages, 1501 KB  
Article
Deciphering Soil Hydro-Physical Controls on Microplastic Fate Using Explainable Machine Learning
by Kübra Polat, Hikmet Günal, Murat Birol, Miraç Kılıç and Mesut Budak
Land 2026, 15(8), 1399; https://doi.org/10.3390/land15081399 - 3 Aug 2026
Viewed by 210
Abstract
Understanding the environmental fate of microplastics (MPs) in agricultural soils remains a major challenge, particularly under field conditions where soil structure and hydraulic processes jointly regulate particle transport and retention. This study investigated whether hydro-physical soil functioning can explain the distribution and accumulation [...] Read more.
Understanding the environmental fate of microplastics (MPs) in agricultural soils remains a major challenge, particularly under field conditions where soil structure and hydraulic processes jointly regulate particle transport and retention. This study investigated whether hydro-physical soil functioning can explain the distribution and accumulation of MPs in pistachio orchard soils from a semi-arid region of southeastern Türkiye. A total of 42 soil samples were analyzed for MP abundance, size distribution, and morphology, together with key hydro-physical properties including texture, porosity, bulk density, aggregate stability, organic matter content, and soil water retention characteristics. To identify the dominant controls on MP occurrence, explainable machine learning approaches combining Random Forest (RF), Gradient Boosting Decision Trees (GBDT), and SHAP (SHapley Additive exPlanations) analysis were employed. Microplastic abundance differed among management systems. Former landfill or construction sites represented the largest proportion of the total recorded microplastic abundance (40.9%), followed by conventionally managed (25.2%), manure-amended (24.5%), and sewage-sludge-amended orchards (9.4%). Median microplastic abundances were 1433, 667, 4633, and 633 particles kg−1 soil, respectively. Fine-sized MPs constituted the dominant particle fraction and exhibited strong associations with pore-system characteristics, indicating that pore-size compatibility governs their retention and mobility within the soil matrix. Morphology-specific analyses further revealed contrasting relationships between soil hydro-physical properties and individual MP forms, suggesting distinct retention pathways for granules, films, fragments, and fibers. Explainable AI analysis identified organic matter, silt content, bulk density, and water retention characteristics as the most influential predictors of MP occurrence. Among the tested models, RF demonstrated superior predictive robustness and generalization capacity. The findings demonstrate that hydro-physical soil functioning plays a central role in determining microplastic fate in agricultural soils and highlight the value of interpretable machine learning frameworks for uncovering the mechanisms underlying contaminant retention and redistribution. Integrating soil structural indicators with explainable artificial intelligence offers a promising pathway for improving microplastic risk assessment in agroecosystems. Full article
(This article belongs to the Special Issue Feature Papers for “Land, Soil and Water” Section, 2nd Edition)
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33 pages, 1922 KB  
Systematic Review
Smart Urban Agriculture in Transition: A Systematic Review of ICT Integration, Applications, and Challenges
by Ruhang Wei, Dan Wu, Wulijiang Mulati, Qifeng Hou and Shengxi Xin
Agriculture 2026, 16(15), 1668; https://doi.org/10.3390/agriculture16151668 - 3 Aug 2026
Viewed by 384
Abstract
This paper systematically reviews the emerging field of smart urban agriculture, defined as the integration of information and communication technologies (ICTs) into food production practices within and around built-up urban areas. Although urban agriculture and digital agriculture have each generated substantial scholarship, their [...] Read more.
This paper systematically reviews the emerging field of smart urban agriculture, defined as the integration of information and communication technologies (ICTs) into food production practices within and around built-up urban areas. Although urban agriculture and digital agriculture have each generated substantial scholarship, their intersection remains conceptually fragmented and empirically uneven. Following the PRISMA 2020 guidelines, this study reviews 143 English-language articles indexed in Web of Science and Scopus between 2016 and 2026, combining bibliometric mapping with structured thematic coding. The analysis shows that smart urban agriculture has expanded rapidly since 2021, but remains geographically concentrated and disciplinarily dispersed. Current research is organized mainly around IoT and sensor networks, machine learning, soilless cultivation, vertical farming, plant factories, and controlled-environment agriculture. ICT applications are most mature in enclosed, data-rich, and technically controllable systems, where they support monitoring, prediction, automation, and resource optimization. By contrast, community-based, open-space, and governance-oriented forms of urban agriculture remain underexplored. By systematically linking ICT families with different urban agriculture production settings, this review clarifies the field’s emerging knowledge structure and demonstrates that technological development remains uneven across agricultural forms and socio-institutional contexts. Full article
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18 pages, 9848 KB  
Article
Real-Time Environmental Monitoring System for Open-Field Avocado Crops
by Mirna Castro-Bello, Mario Alberto Duque-Peralta, Cornelio Morales-Morales, Lizbeth Gómez Muñoz, Vitervo López Caballero, Daniel Angeles-Herrera, Sergio Ricardo Zagal-Barrera and Diego Esteban Gutiérrez-Valencia
AgriEngineering 2026, 8(8), 321; https://doi.org/10.3390/agriengineering8080321 - 1 Aug 2026
Viewed by 263
Abstract
The effects of climate change have altered precipitation patterns, causing extreme weather events that impact crop yields and increase the demand for agricultural production, which drives the need to implement emerging technologies. This research presents the design, construction, and validation of a real-time [...] Read more.
The effects of climate change have altered precipitation patterns, causing extreme weather events that impact crop yields and increase the demand for agricultural production, which drives the need to implement emerging technologies. This research presents the design, construction, and validation of a real-time monitoring system for environmental parameters in open-field avocado orchards. A four-phase methodology was employed: (1) Establishment of required parameters and construction of the IoT architecture; (2) Design of the geometry and final elaboration of the system: modeling in SolidWorks, circuit diagrams in Fritzing, and system assembly; (3) Development of a mobile application: Android Studio and development of the autoencoder model with machine learning; and (4) Validation: Evaluation of the wireless link; system implementation and deployment of the mobile application. The results obtained include a system with a transmitter node equipped with sensors and a receiver, both incorporating ESP32 and nRF24L01+PA+LNA modules, with a wireless transmission range of 2300 m. A total of 2016 data records were stored on microSD and in the cloud, which can be queried, visualized, and analyzed through the mobile application via Bluetooth or Wi-Fi; the system also detects outlier data that can be used for decision-making in the agricultural sector. Full article
(This article belongs to the Topic Digital Agriculture, Smart Farming and Crop Monitoring)
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21 pages, 3435 KB  
Review
Genomic Selection Integrated with High-Throughput Phenotyping and Speed Breeding for Smart and Greener Rice (Oryza sativa) Improvement
by Ha Duc Chu, Trung Quoc Nguyen, Loc Van Nguyen, Nguyen Nguyen Chuong, Quyen Thi Ha, Nguyen Thi Phuong Thao, Touhidur Rahman Anik, Saad Sulieman, Weiqiang Li and Lam-Son Phan Tran
Genes 2026, 17(8), 900; https://doi.org/10.3390/genes17080900 - 30 Jul 2026
Viewed by 367
Abstract
Background: Rice breeding requires faster development of high-yielding, climate-resilient, resource-efficient, and high-quality cultivars for production systems exposed to environmental variability and increasing input constraints. Genomic selection offers an opportunity to predict breeding value before extensive field evaluation, although its effectiveness depends on [...] Read more.
Background: Rice breeding requires faster development of high-yielding, climate-resilient, resource-efficient, and high-quality cultivars for production systems exposed to environmental variability and increasing input constraints. Genomic selection offers an opportunity to predict breeding value before extensive field evaluation, although its effectiveness depends on the integration of genomic, phenotypic, and environmental information. Methods: This narrative review critically examines recent advances in genomic selection for rice and its integration with high-throughput genotyping, high-throughput phenotyping, machine learning, multi-environment prediction, and speed breeding. Results: Genome-wide marker data can support early ranking of breeding materials for grain yield, grain quality, disease resistance, drought tolerance, salinity tolerance, and nutrient-use efficiency. Prediction performance is influenced by trait architecture, marker density, training-population size, genetic relatedness between training and candidate populations, phenotypic data quality, and genotype-by-environment interaction. Red-green-blue, multispectral, hyperspectral, thermal, and light detection and ranging platforms can generate temporal traits associated with plant architecture, biomass, water status, nutrient status, and stress responses, which may improve prediction under suitable population and validation designs. Speed-breeding systems shorten generation intervals and facilitate rapid advancement, recurrent selection, and recycling of superior parental lines. Conclusions: Integrated breeding pipelines that combine genomic prediction, high-throughput phenotyping, environmental data, and speed breeding can improve selection efficiency and shorten rice improvement cycles. Wider adoption will require affordable technology platforms, standardized data systems, multi-environment validation, breeder capacity development, and collaborative data-sharing frameworks for smart and greener agriculture. Full article
(This article belongs to the Special Issue Genomics for Smart and Greener Agriculture)
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47 pages, 3348 KB  
Review
Engineering Plant-Associated Soil Microbiomes for Sustainable and Climate-Resilient Agriculture: Mechanisms, Technologies, and Applications
by Amankeldi K. Sadanov, Gul Baimakhanova, Baiken B. Baimakhanova, Saltanat Orazymbet, Irina Ratnikova, Irina Smirnova, Nurgul Mamytova, Raikhan Sydykbekova, Bekzhan D. Kossalbayev, Gulzat S. Aitkaliyeva and Ayaz M. Belkozhayev
Microorganisms 2026, 14(8), 1648; https://doi.org/10.3390/microorganisms14081648 - 28 Jul 2026
Viewed by 312
Abstract
Soil microbiomes are essential for nutrient cycling, plant health, stress resilience, and sustainable agriculture. Recent advances in high-throughput sequencing, multi-omics technologies, systems biology, and artificial intelligence (AI) have transformed our understanding of plant–microbiome interactions and enabled the development of innovative microbiome engineering strategies. [...] Read more.
Soil microbiomes are essential for nutrient cycling, plant health, stress resilience, and sustainable agriculture. Recent advances in high-throughput sequencing, multi-omics technologies, systems biology, and artificial intelligence (AI) have transformed our understanding of plant–microbiome interactions and enabled the development of innovative microbiome engineering strategies. This review provides a comprehensive overview of the mechanisms governing plant-associated soil microbiome assembly, microbial community functions, plant–microbe communication, and microbiome-mediated stress resistance in agricultural ecosystems. Current approaches to plant-associated soil microbiome manipulation and engineering, including microbial inoculants, synthetic microbial communities (SynComs), microbiome transplantation, rhizosphere steering, and synthetic biology-based interventions, are critically examined. The review further discusses the growing role of metagenomics, metabolomics, metatranscriptomics, machine learning (ML), and precision agriculture technologies in improving microbiome characterization, prediction, and management. Particular attention is given to the application of microbiome-based solutions for sustainable crop production, nutrient management, biological control, climate-smart agriculture, and ecosystem restoration. Despite significant progress, challenges related to field-scale variability, colonization stability, biosafety, regulatory frameworks, and data integration continue to limit large-scale implementation. Future advances in precision microbiome engineering are expected to combine ecological principles, multi-omics technologies, AI, and synthetic biology to develop predictive and resilient microbiome-based solutions for sustainable and climate-resilient agriculture. Full article
(This article belongs to the Special Issue Insect–Plant–Microbe Interactions and Sustainable Agriculture)
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44 pages, 13789 KB  
Review
Integrated Drought Resilience in Foxtail Millet: From Molecular Regulation and Multi-Omics to Climate-Resilient Breeding
by Gan Liu, Shaohua Li, Qi He, Chirui Zhang, Jun Zhang and Zhong Tang
Water 2026, 18(15), 1823; https://doi.org/10.3390/w18151823 - 27 Jul 2026
Viewed by 417
Abstract
Climate change and the increasing frequency of extreme temperatures pose severe threats to global agricultural productivity, making the breeding of water-efficient crops a critical imperative. Originating from arid regions, foxtail millet serves as an ideal C4 model crop for elucidating plant adaptations to [...] Read more.
Climate change and the increasing frequency of extreme temperatures pose severe threats to global agricultural productivity, making the breeding of water-efficient crops a critical imperative. Originating from arid regions, foxtail millet serves as an ideal C4 model crop for elucidating plant adaptations to water deficits. Unlike previous reviews that often isolate genomic features from physiological responses, this review constructs an explicit conceptual framework integrating cross-scale defense mechanisms—mechanistically linking molecular signal transduction and post-transcriptional regulation to cellular homeostasis and field-scale yield stability. We first detail the developmental stage-specific physiological penalties of water stress and dissect proactive water-conservation strategies, including stomatal anatomical optimization, root-carbon reallocation, and dynamic rhizosphere remodeling. At the genetic level, we highlight the application of dynamic quantitative trait loci (QTL) mapping, which transcends the static limitations of conventional QTLs by capturing the spatiotemporal evolution of drought-tolerance traits across distinct developmental nodes. To bridge the gap between intrinsic genetic potential and field application, we spotlight the emerging integration of machine learning-assisted breeding and genomic prediction for the efficient evaluation of superior germplasms. Across this framework, several persistent gaps emerge: most drought-responsive genes identified in foxtail millet remain at the level of expression association without functional validation; dynamic QTL analysis remains underutilized relative to its capacity to resolve reproductive-stage drought tolerance; and ML-based genomic prediction, though demonstrated in this species, has not been integrated into operational breeding. Closing these gaps will require connecting high-throughput field phenotyping to genomic selection and deploying functionally validated editing targets in genetic backgrounds relevant to dryland production. Full article
(This article belongs to the Special Issue Resilient Water Management in Arid and Semi-Arid Agroecosystems)
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41 pages, 7468 KB  
Article
A Comparative Analysis of Dynamic Time Warping and Machine Learning Models for Crop Classification: Case Study of Limarí River Basin, Chile
by Aldo A. Tapia and Andrew Bennett
Earth 2026, 7(4), 122; https://doi.org/10.3390/earth7040122 - 24 Jul 2026
Viewed by 571
Abstract
Crop monitoring is an important aspect of agricultural management, as it provides insights into cultivated area, crop health, growth patterns, and yields potential. Mapping cultivated areas and identifying crop types was historically conducted through field surveys and manual mapping, which are time-consuming and [...] Read more.
Crop monitoring is an important aspect of agricultural management, as it provides insights into cultivated area, crop health, growth patterns, and yields potential. Mapping cultivated areas and identifying crop types was historically conducted through field surveys and manual mapping, which are time-consuming and labor-intensive. Remote sensing classification has transformed large-scale land cover mapping, including crop identification. This work aims to: (1) compare the performance of Dynamic Time Warping (DTW) and two machine learning families (artificial neural networks and decision trees) for crop classification using Sentinel-2 data; (2) assess whether reflectance data, spectral indices, or both yield better classification results; and (3) evaluate the effect of hyperparameters on model performance. Among the DTW variants evaluated, dynamic time warping without a time constraint performed the best, with an overall accuracy of 0.921 using the combination of both reflectance and spectral indices. Most machine learning methods outperformed DTW. Although the convolutional neural network reached the highest single accuracy (0.948), the transformer was selected as the best model overall (accuracy of 0.944), as it combined a comparable accuracy with the lowest sensitivity to hyperparameter variations, making it a reliable option when testing machine learning architectures applied to crop mapping. This work also provides insights for model architecture development based on an exhaustive hyperparameter search for the machine learning models. Full article
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26 pages, 2033 KB  
Article
A Spatiotemporal Multimodal Transformer for Pre-Harvest Apple and Pear Quality Prediction
by Zhengjie Fu, Huaren Shen, Yan Shi, Yiheng Zhang, Luyao Xiao, Ying Li and Min Dong
Agronomy 2026, 16(15), 1399; https://doi.org/10.3390/agronomy16151399 - 23 Jul 2026
Viewed by 358
Abstract
This study addresses the problem of pre-harvest fruit commodity grade prediction in intelligent orchards. To overcome the limitations of conventional post-harvest grading systems, including delayed quality evaluation, the limited representation capability of single-modality approaches, and the difficulty of modeling heterogeneous agricultural information collected [...] Read more.
This study addresses the problem of pre-harvest fruit commodity grade prediction in intelligent orchards. To overcome the limitations of conventional post-harvest grading systems, including delayed quality evaluation, the limited representation capability of single-modality approaches, and the difficulty of modeling heterogeneous agricultural information collected throughout the growing season, this study proposes STF-Former, a spatiotemporal multimodal Transformer framework for pre-harvest fruit quality prediction. Rather than relying solely on static visual observations, the proposed framework formulates fruit quality prediction as a multimodal spatiotemporal learning problem by jointly exploiting phenotypic evolution, environmental dynamics, and field management information across different growth stages. Through stage-aware temporal modeling and cross-modal representation learning, STF-Former captures the dynamic interactions between fruit developmental processes and environmental water–fertilizer conditions, providing a unified prediction framework for both commodity grades and key quality indicators. This design improves the interpretability of quality formation and establishes a methodological framework for integrating heterogeneous agricultural data in precision orchard management. Experimental results demonstrate that the proposed method achieves significant advantages in both classification and regression tasks. In the commodity grade classification task, STF-Former achieves an Accuracy of 0.887, a Precision of 0.875, a Recall of 0.861, a Macro-F1 score of 0.868, and an AUC of 0.924, substantially outperforming traditional machine-learning methods (Random Forest and XGBoost) as well as mainstream unimodal deep-learning models. In the regression task for key quality indicators, superior performance is consistently achieved across multiple agronomic metrics, where the mean absolute error (MAE) is 3.41 mm for fruit diameter, 11.85 g for single fruit weight, 0.057 for coloration index, 0.81 °Brix for soluble solid content, and 2.68 N for firmness, with an overall R2 reaching 0.846. These results validate the effectiveness and robustness of multimodal spatiotemporal learning for accurate pre-harvest fruit quality prediction. The proposed framework provides a practical technical solution for intelligent orchard management, harvest planning, and data-driven precision agriculture, while offering a scalable paradigm for integrating multimodal sensing and temporal learning in smart agricultural systems. Full article
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